FvH4_2g27661

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001778
Physical Location & Seq
Forward (+)
21898371 .. 21901407
3037 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27661.t2

Sequence Viewer

Length: 1434 bp
ATGGAGGACAACACTGCTGCAAGGAACCACATCCATTCCAACCCAGCTTTTGATATCTCAGTTATGGAAGGAGGGTCCAAGTTCTTCGATGATGATGGCCGTCCCAAAAGGACTGGAAATGTTATGACTGCAAGTGCTCACATAATTACTGCCGTCATTGGTTCTGGGGTTCTGTCATTGGCTTGGGCCATTGCTCAGCTTGGCTGGGTTGTTGGTCCTGTTGTGATGGTCATGTTTTCCATAGTGACTTACTACACTTCATGTCTTCTCGTTGCCTGCTATCGTGATCCAGTCACCGGAAATAGAAACTCTACTTACTCGGCTGCTGTTAAAAACCACCTTGGTGGATTTATGTACAAAGTTCTTGGAATTGTTCAGCGTATTAATCTTTACGGAATCACCATTGGCTACACCATAACAGCATGTATAAGCATGGTTGCAATACGAAAGAATGCCTGCGTGCATAAGCATGGTGAAGGAAAACGATGTCTGATAAACAGCAATCCCTATATGATTGCTTTTGGCGTCATAGAAATTATATTATCTCAAATTCCAAATTTTGATAAAATGGCGTGGCTATCTACTGTTGCTGCAATCATGTCATTTACATACTCAGGGATCGGACTTGCCCTTGGAATTGCTAAAGTTGCAGAAATTAAAACAATCAAGGGAAGTCTTACCGGAGTAACCATTGGAGTTCAAGTGACTCAAACCGAAAAGATTTGGAGGATCTTCCAGGCACTTGGTGACATAGCTTTTGCTTACTCATTTGCTATCGTCCTCATTGAAATTGAGGACACGGTTAAATCTCCACCATCAGAAGCCAAGACAATGAAGACAGCCAGTAAAATAAGTTTATCAGTGACATCCATTTTCTACATTTTGTGTGGTTGCTTAGGTTATGCTGCTTTCGGAGATTTAGCCCCCGGAAACCTCCTGACAGGTACAGGCTTCTCTAATCCATCTTGGCTCATTGACATAGCCAATGCTGCCATAGTTATCCATCTTATTGGAGCATACCAAGTCTTCAGCCAGCCCTTTTACGCATTCGTTGAGAAGAAAGCAGCACAGCTTTACCCAGATAACCACTTCATCACAAAAGACATCGAAATCATGTCCTACAAGCTTAACCTTTTTAAAGTGGTCTGGAGGACGCTATATGTGATTTCGACAACTTTGTTATCGATGATCTTCCCGTTCTTCAATGGCGTGGTTGGATTTCTTGGGGCTTTAGGGTACTGGCCAATGACAATCTACTTCCCTGTGGCAATGTACATTGCTCAAAAACAGATACCAAAGTGGAGCACAAGATGGGTTTGCCTCCAAGTTCTATGTCTTTCTGTGCTAGTAATAGCTTTACTAGCTGCTGTTGGCTCGATTGTTGGCGTGGTGGAAAGTCTCAAAGATTACAAGCCATTCATGACCAGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

478

Amino Acids

52.1

Weight (kDa)

9.02

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 38 - 464 1.2e-108 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 281, 626, 737
AcoI YGGCCR 2 cut(s) 97, 1241
AcsI RAATTY 2 cut(s) 549, 556
AcuI CTGAAG 1 cut(s) 1012
AcyI GRCGYC 1 cut(s) 525
AdeI CACNNNGTG 1 cut(s) 746
AfaI GTAC 4 cut(s) 356, 946, 1238, 1274
AfiI CCNNNNNNNGG 1 cut(s) 296
AgsI TTSAA 3 cut(s) 701, 788, 1204
AjnI CCWGG 1 cut(s) 735
AleI CACNNNNGTG 1 cut(s) 342
AluBI AGCT 7 cut(s) 47, 199, 755, 1072, 1126, 1355, 1364
AluI AGCT 7 cut(s) 47, 199, 755, 1072, 1126, 1355, 1364
Alw21I GWGCWC 2 cut(s) 139, 1307
Alw26I GTCTC 1 cut(s) 1403
AlwI GGATC 3 cut(s) 281, 626, 737
AoxI GGCC 3 cut(s) 97, 186, 1241
ApeKI GCWGC 7 cut(s) 17, 323, 590, 905, 989, 1064, 1364
ApoI RAATTY 2 cut(s) 549, 556
ArsI GACNNNNNNTTYG 2 cut(s) 740, 772
AseI ATTAAT 1 cut(s) 384
AspS9I GGNCC 3 cut(s) 75, 186, 215
AsuC2I CCSGG 1 cut(s) 927
AsuHPI GGTGA 4 cut(s) 286, 391, 485, 758
AvaII GGWCC 2 cut(s) 75, 215
BaeI ACNNNNGTAYC 2 cut(s) 1283, 1316
BalI TGGCCA 1 cut(s) 1243
BbsI GAAGAC 3 cut(s) 257, 842, 1018
Bbv12I GWGCWC 2 cut(s) 139, 1307
BbvI GCAGC 7 cut(s) 4, 310, 577, 892, 976, 1076, 1351
BccI CCATC 6 cut(s) 89, 220, 823, 970, 1011, 1305
BceAI ACGGC 2 cut(s) 84, 137
BciT130I CCWGG 1 cut(s) 737
BcnI CCSGG 1 cut(s) 927
BcoDI GTCTC 1 cut(s) 1403
BfaI CTAG 2 cut(s) 1346, 1361
BisI GCNGC 7 cut(s) 18, 324, 591, 906, 990, 1065, 1365
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 7 cut(s) 19, 325, 592, 907, 991, 1066, 1366
Bme1390I CCNGG 2 cut(s) 737, 927
Bme18I GGWCC 2 cut(s) 75, 215
BmgT120I GGNCC 3 cut(s) 75, 186, 215
BmiI GGNNCC 2 cut(s) 26, 76
BmrFI CCNGG 2 cut(s) 737, 927
BpiI GAAGAC 3 cut(s) 257, 842, 1018
BpmI CTGGAG 1 cut(s) 1168
Bpu10I CCTNAGC 1 cut(s) 895
Bpu1102I GCTNAGC 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 927
Bsa29I ATCGAT 1 cut(s) 1184
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 3 cut(s) 340, 631, 925
BsaWI WCCGGW 2 cut(s) 296, 680
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 4 cut(s) 118, 290, 843, 1244
Bse3DI GCAATG 3 cut(s) 189, 1275, 1275
BseBI CCWGG 1 cut(s) 737
BseCI ATCGAT 1 cut(s) 1184
BseDI CCNNGG 3 cut(s) 340, 631, 925
BseGI GGATG 2 cut(s) 30, 866
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMI GCAATG 3 cut(s) 189, 1275, 1275
BseMII CTCAG 3 cut(s) 72, 209, 627
BseNI ACTGG 4 cut(s) 118, 290, 843, 1244
BseXI GCAGC 7 cut(s) 4, 310, 577, 892, 976, 1076, 1351
BseYI CCCAGC 2 cut(s) 43, 204
BshFI GGCC 3 cut(s) 99, 188, 1243
BshVI ATCGAT 1 cut(s) 1184
BsiHKAI GWGCWC 2 cut(s) 139, 1307
BsiSI CCGG 3 cut(s) 297, 681, 927
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 1403
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 2 cut(s) 457, 1046
BsnI GGCC 3 cut(s) 99, 188, 1243
Bsp1286I GDGCHC 2 cut(s) 139, 1307
Bsp1407I TGTACA 2 cut(s) 354, 1272
Bsp143I GATC 4 cut(s) 286, 618, 729, 1188
Bsp1720I GCTNAGC 1 cut(s) 195
BspANI GGCC 3 cut(s) 99, 188, 1243
BspCNI CTCAG 3 cut(s) 71, 208, 626
BspDI ATCGAT 1 cut(s) 1184
BspHI TCATGA 1 cut(s) 1419
BspLI GGNNCC 2 cut(s) 26, 76
BspPI GGATC 3 cut(s) 281, 626, 737
BsrDI GCAATG 3 cut(s) 189, 1275, 1275
BsrGI TGTACA 2 cut(s) 354, 1272
BsrI ACTGG 4 cut(s) 118, 290, 843, 1244
BssECI CCNNGG 3 cut(s) 340, 631, 925
BssMI GATC 4 cut(s) 286, 618, 729, 1188
BssNI GRCGYC 1 cut(s) 525
BssT1I CCWWGG 2 cut(s) 340, 631
Bst2UI CCWGG 1 cut(s) 737
Bst4CI ACNGT 2 cut(s) 586, 802
BstACI GRCGYC 1 cut(s) 525
BstAUI TGTACA 2 cut(s) 354, 1272
BstC8I GCNNGC 4 cut(s) 277, 457, 461, 1034
BstDEI CTNAG 4 cut(s) 58, 195, 613, 895
BstF5I GGATG 2 cut(s) 30, 866
BstKTI GATC 4 cut(s) 289, 621, 732, 1191
BstMAI GTCTC 1 cut(s) 1403
BstMBI GATC 4 cut(s) 286, 618, 729, 1188
BstMWI GCNNNNNNNGC 3 cut(s) 647, 989, 1361
BstNI CCWGG 1 cut(s) 737
BstNSI RCATGY 1 cut(s) 426
BstSCI CCNGG 2 cut(s) 735, 925
BstV1I GCAGC 7 cut(s) 4, 310, 577, 892, 976, 1076, 1351
BstV2I GAAGAC 3 cut(s) 257, 842, 1018
BstX2I RGATCY 1 cut(s) 729
BstXI CCANNNNNNTGG 3 cut(s) 344, 743, 1010
BstYI RGATCY 1 cut(s) 729
Bsu15I ATCGAT 1 cut(s) 1184
BsuRI GGCC 3 cut(s) 99, 188, 1243
BsuTUI ATCGAT 1 cut(s) 1184
BtsCI GGATG 2 cut(s) 30, 866
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 2 cut(s) 12, 867
Cac8I GCNNGC 4 cut(s) 277, 457, 461, 1034
CciI TCATGA 1 cut(s) 1419
Cfr13I GGNCC 3 cut(s) 75, 186, 215
ClaI ATCGAT 1 cut(s) 1184
CseI GACGC 2 cut(s) 514, 1162
Csp6I GTAC 4 cut(s) 355, 945, 1237, 1273
CviAII CATG 8 cut(s) 232, 261, 423, 433, 470, 598, 1114, 1420
CviQI GTAC 4 cut(s) 355, 945, 1237, 1273
DdeI CTNAG 4 cut(s) 58, 195, 613, 895
DpnI GATC 4 cut(s) 288, 620, 731, 1190
DpnII GATC 4 cut(s) 286, 618, 729, 1188
DraI TTTAAA 1 cut(s) 1138
DraIII CACNNNGTG 1 cut(s) 746
EaeI YGGCCR 2 cut(s) 97, 1241
Eco130I CCWWGG 2 cut(s) 340, 631
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 75, 215
Eco57I CTGAAG 1 cut(s) 1012
EcoRII CCWGG 1 cut(s) 735
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 2 cut(s) 340, 631
ErhI CCWWGG 2 cut(s) 340, 631
FaeI CATG 8 cut(s) 235, 264, 426, 436, 473, 601, 1117, 1423
FaqI GGGAC 1 cut(s) 87
FatI CATG 8 cut(s) 231, 260, 422, 432, 469, 597, 1113, 1419
Fnu4HI GCNGC 7 cut(s) 18, 324, 591, 906, 990, 1065, 1365
FokI GGATG 2 cut(s) 17, 853
Fsp4HI GCNGC 7 cut(s) 18, 324, 591, 906, 990, 1065, 1365
FspBI CTAG 2 cut(s) 1346, 1361
GluI GCNGC 7 cut(s) 18, 324, 591, 906, 990, 1065, 1365
GsaI CCCAGC 2 cut(s) 47, 208
GsuI CTGGAG 1 cut(s) 1168
HaeIII GGCC 3 cut(s) 99, 188, 1243
HapII CCGG 3 cut(s) 297, 681, 927
HgaI GACGC 2 cut(s) 514, 1162
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 8 cut(s) 235, 264, 426, 436, 473, 601, 1117, 1423
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 2 cut(s) 396, 706
HpaII CCGG 3 cut(s) 297, 681, 927
HphI GGTGA 4 cut(s) 286, 391, 485, 758
Hpy188I TCNGA 4 cut(s) 492, 623, 820, 914
Hpy188III TCNNGA 4 cut(s) 284, 937, 1147, 1420
HpyAV CCTTC 2 cut(s) 62, 470
HpyCH4III ACNGT 2 cut(s) 586, 802
HpyCH4V TGCA 6 cut(s) 20, 131, 440, 463, 593, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 647, 989, 1361
HpyF3I CTNAG 4 cut(s) 58, 195, 613, 895
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 8 cut(s) 235, 264, 426, 436, 473, 601, 1117, 1423
Kzo9I GATC 4 cut(s) 286, 618, 729, 1188
LmnI GCTCC 2 cut(s) 1013, 1302
Lsp1109I GCAGC 7 cut(s) 4, 310, 577, 892, 976, 1076, 1351
MaeI CTAG 2 cut(s) 1346, 1361
MaeIII GTNAC 6 cut(s) 244, 292, 685, 703, 746, 862
MalI GATC 4 cut(s) 288, 620, 731, 1190
MboI GATC 4 cut(s) 286, 618, 729, 1188
MboII GAAGA 8 cut(s) 76, 257, 724, 847, 1018, 1069, 1183, 1192
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 2 cut(s) 139, 1307
MlsI TGGCCA 1 cut(s) 1243
MluCI AATT 8 cut(s) 144, 369, 534, 549, 556, 636, 654, 789
MluNI TGGCCA 1 cut(s) 1243
MlyI GAGTC 1 cut(s) 700
MmeI TCCRAC 2 cut(s) 63, 1195
MnlI CCTC 7 cut(s) 65, 720, 787, 791, 944, 1143, 1331
Mox20I TGGCCA 1 cut(s) 1243
MscI TGGCCA 1 cut(s) 1243
MseI TTAA 6 cut(s) 330, 384, 657, 804, 1128, 1137
MslI CAYNNNNRTG 2 cut(s) 342, 468
Msp20I TGGCCA 1 cut(s) 1243
MspI CCGG 3 cut(s) 297, 681, 927
MspR9I CCNGG 2 cut(s) 737, 927
Mva1269I GAATGC 2 cut(s) 457, 1046
MvaI CCWGG 1 cut(s) 737
MwoI GCNNNNNNNGC 3 cut(s) 647, 989, 1361
NciI CCSGG 1 cut(s) 927
NdeII GATC 4 cut(s) 286, 618, 729, 1188
NlaIII CATG 8 cut(s) 235, 264, 426, 436, 473, 601, 1117, 1423
NlaIV GGNNCC 2 cut(s) 26, 76
NmeAIII GCCGAG 1 cut(s) 299
NmuCI GTSAC 5 cut(s) 244, 292, 703, 746, 862
NspI RCATGY 1 cut(s) 426
OliI CACNNNNGTG 1 cut(s) 342
PagI TCATGA 1 cut(s) 1419
PctI GAATGC 2 cut(s) 457, 1046
PfeI GAWTC 1 cut(s) 396
PkrI GCNGC 7 cut(s) 19, 325, 592, 907, 991, 1066, 1366
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
PshBI ATTAAT 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 735
PspFI CCCAGC 2 cut(s) 43, 204
PspGI CCWGG 1 cut(s) 735
PspN4I GGNNCC 2 cut(s) 26, 76
PspPI GGNCC 3 cut(s) 75, 186, 215
PsuI RGATCY 1 cut(s) 729
RsaI GTAC 4 cut(s) 356, 946, 1238, 1274
RsaNI GTAC 4 cut(s) 355, 945, 1237, 1273
RseI CAYNNNNRTG 2 cut(s) 342, 468
SaqAI TTAA 6 cut(s) 330, 384, 657, 804, 1128, 1137
SatI GCNGC 7 cut(s) 18, 324, 591, 906, 990, 1065, 1365
Sau3AI GATC 4 cut(s) 286, 618, 729, 1188
Sau96I GGNCC 3 cut(s) 75, 186, 215
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 2 cut(s) 737, 927
SduI GDGCHC 2 cut(s) 139, 1307
SinI GGWCC 2 cut(s) 75, 215
SmiMI CAYNNNNRTG 2 cut(s) 342, 468
Sse9I AATT 8 cut(s) 144, 369, 534, 549, 556, 636, 654, 789
SspMI CTAG 2 cut(s) 1346, 1361
StyD4I CCNGG 2 cut(s) 735, 925
StyI CCWWGG 2 cut(s) 340, 631
TaaI ACNGT 2 cut(s) 586, 802
TaqI TCGA 5 cut(s) 87, 1107, 1169, 1184, 1376
TasI AATT 8 cut(s) 144, 369, 534, 549, 556, 636, 654, 789
TatI WGTACW 2 cut(s) 354, 1272
TfiI GAWTC 1 cut(s) 396
Tru1I TTAA 6 cut(s) 330, 384, 657, 804, 1128, 1137
Tru9I TTAA 6 cut(s) 330, 384, 657, 804, 1128, 1137
TscAI CASTG 2 cut(s) 19, 867
TseFI GTSAC 5 cut(s) 244, 292, 703, 746, 862
TseI GCWGC 7 cut(s) 17, 323, 590, 905, 989, 1064, 1364
Tsp45I GTSAC 5 cut(s) 244, 292, 703, 746, 862
TspDTI ATGAA 4 cut(s) 249, 848, 1081, 1408
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 2 cut(s) 19, 867
VpaK11BI GGWCC 2 cut(s) 75, 215
VspI ATTAAT 1 cut(s) 384
XapI RAATTY 2 cut(s) 549, 556
XceI RCATGY 1 cut(s) 426
XspI CTAG 2 cut(s) 1346, 1361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.