MD15G1343600.v1.1

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001778
Physical Location & Seq
Reverse (-)
39958042 .. 39960249
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1343600.v1.1.491

Sequence Viewer

Length: 1542 bp
ATGAGTGAAAACACTGCCACAAGGAACCAGCTGCAGCTCCATTACAACCCAGTTTTTGATGTCTCACTCAATGTGCAAGGAGGCTCCAAGTACATTGATGACGATGGCCGTCCCAAACGAACCGGTAAATTATACTTTCTCTCCTTGAGACTGCTAAGCATGTTGAATTGTTCGTCATTTTTTTTTTCATGTGTGTTGGTTGTGGGACAAGGAACTGTTTGGACTGCAAGTTCACACATAATTACTGCTATAATTGGGTCTGGAGTTCTGTCATTGGCTTGGGCCATAGCTCAGCTTGGATGGATTGCTGGTCCTGCTGCGATGGCCATCTTCTCCTTCATCACTTACTACACTTCAACTCTTCTGGCTTCTTGCTATCGTGATCCCGTCACTGGAAAGAGAAACTATAGTTACTCCGATGCCGTTCGATCCAACCTTGGTGAATTTCTGGTCAAAATATGTGGATTTGTTCAGCAATTGAACCTTTTTGGAATTACTATTGGCTATACTGTAGCAGCATCTATAAGCATGATGGCAATAAAGAGGTCTAATTGCTTCCACAATAGTGGTGGAAAAGATCCATGTCATATCAACTACAATCCCTATATGATAGCTTTTGGAACCTCAGAAATTATATTGTCTCAAATTCCAAACTTTGATAAGTTGTCCTGGCTCTCCGTTGTTGCTGCAGTCATGTCCTTCACTTACTCAGGAATTGGACTTGCCCTTGGAATTGCTAAAGTTGCTGAAAATGAAAAAATCAATGGAAGTAGGACTGGAATAAGCCTTGGGATTGTGACTCCTGCCCAAAAGATGTGGAGGGCCTTCCAAGCACTTGGTAACATAGCTTTTGCCTACTCTTACTCAATCATCCTCATTGAAATTCAGGACACAGTTAAATCTCCACCATCTGAAACCAAGACAATGAAGAAGGCCACCCGACTTAGCTTGACAGTGACATCCATTTTCTACATTCTATGTGGCTGCATGGGCTACGCTGCTTTTGGAGACTTAGCCCCTGGAAACCTTCTCACTGACAAAGGCTTCTCTAACCCATTTTGGCTTATCGACATAGCCAACGCCGCCATTGTGATCCACCTTGTTGGTGCATATCAAGTTTTTGCCCAACCCATTTTCGCATTGGTCGAAAAAACAACTACAAAAAATTTCCCAAACAGCCAATTCGTCACGAAAGACATCAAAATCCAGATCCCAGGCTTCAGTTACTACACCCTTAACCTCTTCAGATTGGTTTGGAGGACAATTTTTGTTATTCTAACCACCGTGATCTCCATGATCTTTCCGTTCTTTAACGACATTGTTGGATTTCTTGGGGCCTTAGGATTTTGGCCACTCACAGTTTACTTCCCTGTGGAGATGTATATTGCTCAAAAGAAGATACCAATGTGGAGCACAAGATGGATGTGCCTCCAAACTCTAAGTCTTTGTGTCCTTATAATTGCCCTATCTGCTGCAGCTGGCTCAGTTACTGGTGTGATTCACAATCTCAAGGTCTACAGGCCATTCAAGACTAGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

514

Amino Acids

56.54

Weight (kDa)

9.33

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 70 - 500 1.3e-120 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1457
AccI GTMKAC 1 cut(s) 1515
AciI CCGC 1 cut(s) 1083
AclWI GGATC 5 cut(s) 377, 423, 572, 1087, 1204
AcoI YGGCCR 3 cut(s) 106, 324, 1349
AcsI RAATTY 4 cut(s) 443, 645, 882, 1165
AcuI CTGAAG 2 cut(s) 1204, 1228
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 392
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 5 cut(s) 166, 357, 481, 881, 1528
AjnI CCWGG 3 cut(s) 668, 1018, 1213
AleI CACNNNNGTG 1 cut(s) 564
AloI GAACNNNNNNTCC 2 cut(s) 214, 246
AluBI AGCT 8 cut(s) 31, 37, 290, 295, 614, 848, 948, 1478
AluI AGCT 8 cut(s) 31, 37, 290, 295, 614, 848, 948, 1478
Alw21I GWGCWC 1 cut(s) 1415
Alw26I GTCTC 4 cut(s) 67, 142, 645, 1002
AlwI GGATC 5 cut(s) 377, 423, 572, 1087, 1204
AlwNI CAGNNNCTG 1 cut(s) 1490
AoxI GGCC 8 cut(s) 106, 282, 324, 822, 933, 1335, 1349, 1520
ApeKI GCWGC 9 cut(s) 31, 34, 317, 515, 686, 984, 998, 1472, 1475
ApoI RAATTY 4 cut(s) 443, 645, 882, 1165
ArsI GACNNNNNNTTYG 2 cut(s) 1426, 1458
AsiGI ACCGGT 1 cut(s) 122
AspS9I GGNCC 4 cut(s) 282, 311, 822, 1335
AsuHPI GGTGA 1 cut(s) 452
AvaII GGWCC 1 cut(s) 311
AxyI CCTNAGG 1 cut(s) 1339
BaeI ACNNNNGTAYC 2 cut(s) 1391, 1424
BalI TGGCCA 2 cut(s) 326, 1351
Bbv12I GWGCWC 1 cut(s) 1415
BbvI GCAGC 9 cut(s) 18, 46, 304, 527, 673, 971, 985, 1459, 1487
BccI CCATC 7 cut(s) 98, 294, 316, 335, 526, 916, 1413
BceAI ACGGC 2 cut(s) 93, 407
BciT130I CCWGG 3 cut(s) 670, 1020, 1215
BcoDI GTCTC 4 cut(s) 67, 142, 645, 1002
BfaI CTAG 1 cut(s) 1533
BfmI CTRYAG 6 cut(s) 32, 406, 510, 687, 1473, 1516
BlpI GCTNAGC 2 cut(s) 155, 291
Bme1390I CCNGG 3 cut(s) 670, 1020, 1215
Bme18I GGWCC 1 cut(s) 311
BmgT120I GGNCC 4 cut(s) 282, 311, 822, 1335
BmiI GGNNCC 4 cut(s) 26, 85, 622, 1336
BmrFI CCNGG 3 cut(s) 670, 1020, 1215
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 2 cut(s) 409, 527
BmuI ACTGGG 1 cut(s) 44
BpmI CTGGAG 1 cut(s) 282
Bpu1102I GCTNAGC 2 cut(s) 155, 291
BpuEI CTTGAG 2 cut(s) 166, 1493
BsaBI GATNNNNATC 1 cut(s) 326
BsaJI CCNNGG 5 cut(s) 436, 727, 787, 1018, 1213
BsaWI WCCGGW 1 cut(s) 122
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 4 cut(s) 50, 397, 781, 1495
Bse21I CCTNAGG 1 cut(s) 1339
Bse8I GATNNNNATC 1 cut(s) 326
BseBI CCWGG 3 cut(s) 670, 1020, 1215
BseDI CCNNGG 5 cut(s) 436, 727, 787, 1018, 1213
BseGI GGATG 4 cut(s) 305, 870, 959, 1428
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMII CTCAG 4 cut(s) 305, 639, 723, 1497
BseNI ACTGG 4 cut(s) 50, 397, 781, 1495
BseXI GCAGC 9 cut(s) 18, 46, 304, 527, 673, 971, 985, 1459, 1487
BshFI GGCC 8 cut(s) 108, 284, 326, 824, 935, 1337, 1351, 1522
BshTI ACCGGT 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 1415
BsiSI CCGG 1 cut(s) 123
BslFI GGGAC 2 cut(s) 96, 219
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 4 cut(s) 67, 142, 645, 1002
BsmFI GGGAC 2 cut(s) 96, 219
BsnI GGCC 8 cut(s) 108, 284, 326, 824, 935, 1337, 1351, 1522
Bsp1286I GDGCHC 1 cut(s) 1415
Bsp143I GATC 7 cut(s) 382, 428, 577, 1092, 1209, 1287, 1296
Bsp1720I GCTNAGC 2 cut(s) 155, 291
BspACI CCGC 1 cut(s) 1083
BspANI GGCC 8 cut(s) 108, 284, 326, 824, 935, 1337, 1351, 1522
BspCNI CTCAG 4 cut(s) 304, 638, 722, 1496
BspLI GGNNCC 4 cut(s) 26, 85, 622, 1336
BspMAI CTGCAG 3 cut(s) 36, 691, 1477
BspPI GGATC 5 cut(s) 377, 423, 572, 1087, 1204
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 4 cut(s) 50, 397, 781, 1495
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 5 cut(s) 436, 727, 787, 1018, 1213
BssMI GATC 7 cut(s) 382, 428, 577, 1092, 1209, 1287, 1296
BssT1I CCWWGG 3 cut(s) 436, 727, 787
Bst2UI CCWGG 3 cut(s) 670, 1020, 1215
Bst4CI ACNGT 6 cut(s) 217, 511, 895, 955, 1285, 1360
Bst6I CTCTTC 2 cut(s) 366, 1247
BstC8I GCNNGC 1 cut(s) 1480
BstDEI CTNAG 9 cut(s) 155, 291, 625, 709, 944, 1012, 1339, 1439, 1483
BstF5I GGATG 4 cut(s) 305, 870, 959, 1428
BstKTI GATC 7 cut(s) 385, 431, 580, 1095, 1212, 1290, 1299
BstMAI GTCTC 4 cut(s) 67, 142, 645, 1002
BstMBI GATC 7 cut(s) 382, 428, 577, 1092, 1209, 1287, 1296
BstMWI GCNNNNNNNGC 7 cut(s) 314, 323, 743, 830, 990, 1082, 1469
BstNI CCWGG 3 cut(s) 670, 1020, 1215
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 3 cut(s) 668, 1018, 1213
BstSFI CTRYAG 6 cut(s) 32, 406, 510, 687, 1473, 1516
BstV1I GCAGC 9 cut(s) 18, 46, 304, 527, 673, 971, 985, 1459, 1487
BstX2I RGATCY 2 cut(s) 577, 1209
BstXI CCANNNNNNTGG 3 cut(s) 566, 836, 1103
BstYI RGATCY 2 cut(s) 577, 1209
Bsu36I CCTNAGG 1 cut(s) 1339
BsuRI GGCC 8 cut(s) 108, 284, 326, 824, 935, 1337, 1351, 1522
BtgZI GCGATG 1 cut(s) 335
BtsCI GGATG 4 cut(s) 305, 870, 959, 1428
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 4 cut(s) 12, 390, 960, 1032
Cac8I GCNNGC 1 cut(s) 1480
CaiI CAGNNNCTG 1 cut(s) 1490
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 4 cut(s) 282, 311, 822, 1335
Csp6I GTAC 1 cut(s) 91
CspAI ACCGGT 1 cut(s) 122
CspCI CAANNNNNGTGG 6 cut(s) 442, 477, 550, 585, 797, 832
CviAII CATG 7 cut(s) 160, 189, 529, 582, 694, 988, 1294
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 9 cut(s) 155, 291, 625, 709, 944, 1012, 1339, 1439, 1483
DpnI GATC 7 cut(s) 384, 430, 579, 1094, 1211, 1289, 1298
DpnII GATC 7 cut(s) 382, 428, 577, 1092, 1209, 1287, 1296
EaeI YGGCCR 3 cut(s) 106, 324, 1349
Eam1104I CTCTTC 2 cut(s) 366, 1247
EarI CTCTTC 2 cut(s) 366, 1247
Eco130I CCWWGG 3 cut(s) 436, 727, 787
Eco47I GGWCC 1 cut(s) 311
Eco57I CTGAAG 2 cut(s) 1204, 1228
Eco81I CCTNAGG 1 cut(s) 1339
EcoO109I RGGNCCY 2 cut(s) 822, 1335
EcoRII CCWGG 3 cut(s) 668, 1018, 1213
EcoT14I CCWWGG 3 cut(s) 436, 727, 787
ErhI CCWWGG 3 cut(s) 436, 727, 787
FaeI CATG 7 cut(s) 163, 192, 532, 585, 697, 991, 1297
FaqI GGGAC 2 cut(s) 96, 219
FatI CATG 7 cut(s) 159, 188, 528, 581, 693, 987, 1293
FblI GTMKAC 1 cut(s) 1515
FokI GGATG 4 cut(s) 312, 857, 946, 1435
FspBI CTAG 1 cut(s) 1533
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 8 cut(s) 108, 284, 326, 824, 935, 1337, 1351, 1522
HapII CCGG 1 cut(s) 123
Hin1II CATG 7 cut(s) 163, 192, 532, 585, 697, 991, 1297
HinfI GANTC 2 cut(s) 799, 1498
HpaII CCGG 1 cut(s) 123
HphI GGTGA 1 cut(s) 452
Hpy166II GTNNAC 3 cut(s) 233, 1363, 1516
Hpy188I TCNGA 4 cut(s) 418, 628, 913, 1247
Hpy188III TCNNGA 7 cut(s) 261, 380, 711, 887, 1189, 1207, 1528
Hpy8I GTNNAC 3 cut(s) 233, 1363, 1516
HpyAV CCTTC 5 cut(s) 346, 709, 835, 925, 1037
HpyCH4III ACNGT 6 cut(s) 217, 511, 895, 955, 1285, 1360
HpyCH4V TGCA 7 cut(s) 34, 76, 227, 689, 987, 1109, 1475
HpyF10VI GCNNNNNNNGC 7 cut(s) 314, 323, 743, 830, 990, 1082, 1469
HpyF3I CTNAG 9 cut(s) 155, 291, 625, 709, 944, 1012, 1339, 1439, 1483
Hsp92II CATG 7 cut(s) 163, 192, 532, 585, 697, 991, 1297
Kzo9I GATC 7 cut(s) 382, 428, 577, 1092, 1209, 1287, 1296
LmnI GCTCC 3 cut(s) 42, 89, 1410
Lsp1109I GCAGC 9 cut(s) 18, 46, 304, 527, 673, 971, 985, 1459, 1487
LweI GCATC 2 cut(s) 409, 527
MaeI CTAG 1 cut(s) 1533
MaeIII GTNAC 8 cut(s) 388, 410, 796, 839, 955, 1186, 1223, 1486
MalI GATC 7 cut(s) 384, 430, 579, 1094, 1211, 1289, 1298
MboI GATC 7 cut(s) 382, 428, 577, 1092, 1209, 1287, 1296
MboII GAAGA 5 cut(s) 322, 353, 940, 1234, 1408
MfeI CAATTG 1 cut(s) 476
MflI RGATCY 2 cut(s) 577, 1209
MhlI GDGCHC 1 cut(s) 1415
MlsI TGGCCA 2 cut(s) 326, 1351
MluNI TGGCCA 2 cut(s) 326, 1351
MlyI GAGTC 1 cut(s) 793
MmeI TCCRAC 2 cut(s) 456, 1303
MnlI CCTC 8 cut(s) 74, 537, 634, 813, 884, 1250, 1251, 1439
Mox20I TGGCCA 2 cut(s) 326, 1351
MscI TGGCCA 2 cut(s) 326, 1351
MseI TTAA 4 cut(s) 897, 1236, 1311, 1540
MslI CAYNNNNRTG 1 cut(s) 564
Msp20I TGGCCA 2 cut(s) 326, 1351
MspA1I CMGCKG 2 cut(s) 31, 1478
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 3 cut(s) 670, 1020, 1215
MunI CAATTG 1 cut(s) 476
MvaI CCWGG 3 cut(s) 670, 1020, 1215
MwoI GCNNNNNNNGC 7 cut(s) 314, 323, 743, 830, 990, 1082, 1469
NdeII GATC 7 cut(s) 382, 428, 577, 1092, 1209, 1287, 1296
NlaIII CATG 7 cut(s) 163, 192, 532, 585, 697, 991, 1297
NlaIV GGNNCC 4 cut(s) 26, 85, 622, 1336
NmuCI GTSAC 4 cut(s) 388, 796, 955, 1186
NspI RCATGY 1 cut(s) 163
OliI CACNNNNGTG 1 cut(s) 564
PcsI WCGNNNNNNNCGW 1 cut(s) 1143
PfeI GAWTC 1 cut(s) 1498
PinAI ACCGGT 1 cut(s) 122
PleI GAGTC 1 cut(s) 793
PpsI GAGTC 1 cut(s) 793
PsiI TTATAA 1 cut(s) 1457
Psp6I CCWGG 3 cut(s) 668, 1018, 1213
PspGI CCWGG 3 cut(s) 668, 1018, 1213
PspN4I GGNNCC 4 cut(s) 26, 85, 622, 1336
PspPI GGNCC 4 cut(s) 282, 311, 822, 1335
PstI CTGCAG 3 cut(s) 36, 691, 1477
PstNI CAGNNNCTG 1 cut(s) 1490
PsuI RGATCY 2 cut(s) 577, 1209
PvuII CAGCTG 2 cut(s) 31, 1478
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 564
SaqAI TTAA 4 cut(s) 897, 1236, 1311, 1540
Sau3AI GATC 7 cut(s) 382, 428, 577, 1092, 1209, 1287, 1296
Sau96I GGNCC 4 cut(s) 282, 311, 822, 1335
SchI GAGTC 1 cut(s) 793
ScrFI CCNGG 3 cut(s) 670, 1020, 1215
SduI GDGCHC 1 cut(s) 1415
SfaNI GCATC 2 cut(s) 409, 527
SfcI CTRYAG 6 cut(s) 32, 406, 510, 687, 1473, 1516
SinI GGWCC 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 564
SmlI CTYRAG 2 cut(s) 145, 1508
SmoI CTYRAG 2 cut(s) 145, 1508
SsiI CCGC 1 cut(s) 1083
SspMI CTAG 1 cut(s) 1533
StyD4I CCNGG 3 cut(s) 668, 1018, 1213
StyI CCWWGG 3 cut(s) 436, 727, 787
TaaI ACNGT 6 cut(s) 217, 511, 895, 955, 1285, 1360
TaqI TCGA 3 cut(s) 427, 1068, 1146
TatI WGTACW 1 cut(s) 90
TauI GCSGC 1 cut(s) 1085
TfiI GAWTC 1 cut(s) 1498
Tru1I TTAA 4 cut(s) 897, 1236, 1311, 1540
Tru9I TTAA 4 cut(s) 897, 1236, 1311, 1540
TscAI CASTG 4 cut(s) 19, 397, 960, 1039
TseFI GTSAC 4 cut(s) 388, 796, 955, 1186
TseI GCWGC 9 cut(s) 31, 34, 317, 515, 686, 984, 998, 1472, 1475
Tsp45I GTSAC 4 cut(s) 388, 796, 955, 1186
TspDTI ATGAA 4 cut(s) 177, 328, 768, 941
TspGWI ACGGA 2 cut(s) 667, 1293
TspRI CASTG 4 cut(s) 19, 397, 960, 1039
VpaK11BI GGWCC 1 cut(s) 311
XapI RAATTY 4 cut(s) 443, 645, 882, 1165
XceI RCATGY 1 cut(s) 163
XcmI CCANNNNNNNNNTGG 2 cut(s) 566, 1138
XmiI GTMKAC 1 cut(s) 1515
XspI CTAG 1 cut(s) 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.