Rroxscaffold_7G00178650

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001778
Physical Location & Seq
Forward (+)
18302233 .. 18304254
2022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00178650.1

Sequence Viewer

Length: 1359 bp
ATGGAGGACAACACTGCTTCAAGGAACCATATTCATTCCAACCCAGCTTTTGATGCCTCAGTTATGGATGGAGGCTCCAAGTTCTTTGATGATGATGGCCGTCCCAAAAGGACTGGAAATGTTATGACTGCAAGTGCTCACATAATTACTGCTGTGATTGGTTCTGGGGTTTTGTCATTGGCTTGGGCCATTGCTCAGCTTGGCTGGGTTGTTGGTCCTGTTGTGATGGTCATGTTTTCCTTAGTGACCTACTACACTTCATGTCTTCTCGTTGCCTGCTATCGTGACCCGGTCACTGGAAAAAGAAACTCTACTTACTCGGCTGCTGTTCGAAACCACCTTGGTGGACTTAAGTACAAAGTTCTTGGAATAGTTCAGCGCGTGAACCTTTTCGGAATCACCATTGGCTACACCATAACAGCATGTATAAGCATGGTTGCAATACAAAGGAATTCCTGCTTGCACAAGAGTGGTGGAGTAAAACCATGCCCGGTAAACAGCAATCCCTATATGATTGCTTTTGGCATCACAGAAATTATATTATCTCAAATTCCAAATTTCGATAAGCTGTGGTGGCTATCTACTGTTGCTGCAGTCATGTCATTTACATACTCAGGGATTGGACTTGCCCTTGGAATTGCTAAAGTTGCAGAAAGTGGAACAATGAAGGGAACTCTTACTGGAGTACGCATTGGAATTCATGTGACTCAAACCGAAAAGATATGGAGGATCTTCCAGGCACTTGGTGACATAGCTTTTGCTTACTCATTTGCTATAGTCCTCATTGAAATTGAGGACACAGTTAAATCTCCACCATCAGAAGCCAAGACAATGAAGACGGCCAGTAAAATTAGTTTAGCAGTCACATCCGTCTTCTACATTTTGTGTGGTTGCTTCGGTTATGCTGTTGTCCATCTTGTTGGAGCATTCCAAGTATTCAGCCAGCCCATTTACGCATTCGTTGAGAAAAAAGCAGCACAGCTTTTCCCAAATAGCCACTTCATCACAAAAGAAATCCAAATCCCCACCCCTTGTTTCAAGCCTTACAAGCTTAACCTTTTTAGACTGGTCTGGAGGACACTTTATGTGACTGCAACCACTGTGTTATCGATGGTCTTCCCGTTCTTCAACGGCGTGGTTGGATTTCTTGGGGCTTTGGGGTACTGGCCAATGACAATTTACTTCCCTGTGGAGATGTACATTGCTCAAAAGAAGATACCAAAGTGGAGCACAAGATGGCTTTGCCTCCAAACTCTAAGTCTTTCTGTGCTAATTATAGCTTTACTAGCAGCTGTTGGCTCGATTGCCGGTGTGATTGCAAGTCTCAAAGATTACAAGCCATTCAAGACCAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

452

Amino Acids

49.42

Weight (kDa)

9.48

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 38 - 303 8.5e-71 Transmembrane amino acid transporter protein
Aa_trans PF01490 303 - 439 4.1e-27 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 381
AclWI GGATC 1 cut(s) 737
AcoI YGGCCR 3 cut(s) 97, 840, 1166
AcsI RAATTY 4 cut(s) 451, 549, 556, 696
AdeI CACNNNGTG 1 cut(s) 746
AfaI GTAC 4 cut(s) 356, 687, 1163, 1199
AfiI CCNNNNNNNGG 1 cut(s) 296
AflII CTTAAG 1 cut(s) 350
AgsI TTSAA 5 cut(s) 21, 788, 1039, 1129, 1345
AjnI CCWGG 1 cut(s) 735
AleI CACNNNNGTG 2 cut(s) 342, 468
AluBI AGCT 9 cut(s) 47, 199, 568, 755, 982, 1051, 1280, 1292, 1353
AluI AGCT 9 cut(s) 47, 199, 568, 755, 982, 1051, 1280, 1292, 1353
Alw21I GWGCWC 2 cut(s) 139, 1232
Alw26I GTCTC 1 cut(s) 1328
AlwI GGATC 1 cut(s) 737
AoxI GGCC 4 cut(s) 97, 186, 840, 1166
ApeKI GCWGC 4 cut(s) 323, 590, 974, 1289
ApoI RAATTY 4 cut(s) 451, 549, 556, 696
ArsI GACNNNNNNTTYG 4 cut(s) 740, 772, 1243, 1275
Asp700I GAANNNNTTC 1 cut(s) 389
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 2 cut(s) 186, 215
AsuC2I CCSGG 2 cut(s) 290, 491
AsuHPI GGTGA 2 cut(s) 391, 758
AsuII TTCGAA 1 cut(s) 331
AvaII GGWCC 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 1208, 1241
BalI TGGCCA 1 cut(s) 1168
BbsI GAAGAC 4 cut(s) 257, 842, 865, 1108
Bbv12I GWGCWC 2 cut(s) 139, 1232
BbvI GCAGC 4 cut(s) 310, 577, 986, 1301
BccI CCATC 7 cut(s) 62, 89, 220, 823, 921, 1105, 1230
BceAI ACGGC 3 cut(s) 84, 855, 1147
BciT130I CCWGG 1 cut(s) 737
BcnI CCSGG 2 cut(s) 290, 491
BcoDI GTCTC 1 cut(s) 1328
BfaI CTAG 1 cut(s) 1286
BfmI CTRYAG 2 cut(s) 591, 774
BfrI CTTAAG 1 cut(s) 350
BisI GCNGC 4 cut(s) 324, 591, 975, 1290
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 4 cut(s) 325, 592, 976, 1291
Bme1390I CCNGG 3 cut(s) 290, 491, 737
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 2 cut(s) 186, 215
BmiI GGNNCC 2 cut(s) 26, 76
BmrFI CCNGG 3 cut(s) 290, 491, 737
BmsI GCATC 2 cut(s) 43, 534
BpiI GAAGAC 4 cut(s) 257, 842, 865, 1108
BpmI CTGGAG 2 cut(s) 702, 1093
Bpu1102I GCTNAGC 1 cut(s) 195
Bpu14I TTCGAA 1 cut(s) 331
BpuMI CCSGG 2 cut(s) 290, 491
Bsa29I ATCGAT 1 cut(s) 1109
BsaJI CCNNGG 2 cut(s) 340, 631
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse118I RCCGGY 1 cut(s) 1307
Bse1I ACTGG 6 cut(s) 118, 301, 685, 843, 1071, 1169
Bse3DI GCAATG 2 cut(s) 189, 1200
BseBI CCWGG 1 cut(s) 737
BseCI ATCGAT 1 cut(s) 1109
BseDI CCNNGG 2 cut(s) 340, 631
BseGI GGATG 2 cut(s) 73, 866
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMI GCAATG 2 cut(s) 189, 1200
BseMII CTCAG 3 cut(s) 72, 209, 627
BseNI ACTGG 6 cut(s) 118, 301, 685, 843, 1071, 1169
BseXI GCAGC 4 cut(s) 310, 577, 986, 1301
BseYI CCCAGC 2 cut(s) 43, 204
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 4 cut(s) 99, 188, 842, 1168
BshVI ATCGAT 1 cut(s) 1109
BsiHKAI GWGCWC 2 cut(s) 139, 1232
BsiSI CCGG 3 cut(s) 290, 491, 1308
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 1328
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 2 cut(s) 926, 956
BsnI GGCC 4 cut(s) 99, 188, 842, 1168
Bsp119I TTCGAA 1 cut(s) 331
Bsp1286I GDGCHC 2 cut(s) 139, 1232
Bsp1407I TGTACA 1 cut(s) 1197
Bsp143I GATC 1 cut(s) 729
Bsp1720I GCTNAGC 1 cut(s) 195
BspANI GGCC 4 cut(s) 99, 188, 842, 1168
BspCNI CTCAG 3 cut(s) 71, 208, 626
BspDI ATCGAT 1 cut(s) 1109
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 26, 76
BspMAI CTGCAG 1 cut(s) 595
BspPI GGATC 1 cut(s) 737
BspT104I TTCGAA 1 cut(s) 331
BspTI CTTAAG 1 cut(s) 350
BsrDI GCAATG 2 cut(s) 189, 1200
BsrFI RCCGGY 1 cut(s) 1307
BsrGI TGTACA 1 cut(s) 1197
BsrI ACTGG 6 cut(s) 118, 301, 685, 843, 1071, 1169
BssAI RCCGGY 1 cut(s) 1307
BssECI CCNNGG 2 cut(s) 340, 631
BssMI GATC 1 cut(s) 729
BssT1I CCWWGG 2 cut(s) 340, 631
Bst2UI CCWGG 1 cut(s) 737
Bst4CI ACNGT 3 cut(s) 586, 802, 1102
BstAFI CTTAAG 1 cut(s) 350
BstAUI TGTACA 1 cut(s) 1197
BstBI TTCGAA 1 cut(s) 331
BstC8I GCNNGC 3 cut(s) 277, 461, 944
BstDEI CTNAG 5 cut(s) 58, 195, 241, 613, 1256
BstF5I GGATG 2 cut(s) 73, 866
BstFNI CGCG 1 cut(s) 381
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 1 cut(s) 732
BstMAI GTCTC 1 cut(s) 1328
BstMBI GATC 1 cut(s) 729
BstMWI GCNNNNNNNGC 5 cut(s) 53, 574, 647, 1048, 1286
BstNI CCWGG 1 cut(s) 737
BstNSI RCATGY 1 cut(s) 426
BstSCI CCNGG 3 cut(s) 288, 489, 735
BstSFI CTRYAG 2 cut(s) 591, 774
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 4 cut(s) 310, 577, 986, 1301
BstV2I GAAGAC 4 cut(s) 257, 842, 865, 1108
BstX2I RGATCY 1 cut(s) 729
BstXI CCANNNNNNTGG 3 cut(s) 344, 743, 920
BstYI RGATCY 1 cut(s) 729
Bsu15I ATCGAT 1 cut(s) 1109
BsuRI GGCC 4 cut(s) 99, 188, 842, 1168
BsuTUI ATCGAT 1 cut(s) 1109
BtsCI GGATG 2 cut(s) 73, 866
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 3 cut(s) 12, 294, 1098
Cac8I GCNNGC 3 cut(s) 277, 461, 944
CfoI GCGC 1 cut(s) 381
Cfr10I RCCGGY 1 cut(s) 1307
Cfr13I GGNCC 2 cut(s) 186, 215
ClaI ATCGAT 1 cut(s) 1109
Csp6I GTAC 4 cut(s) 355, 686, 1162, 1198
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 7 cut(s) 232, 261, 423, 433, 486, 598, 701
CviQI GTAC 4 cut(s) 355, 686, 1162, 1198
DdeI CTNAG 5 cut(s) 58, 195, 241, 613, 1256
DpnI GATC 1 cut(s) 731
DpnII GATC 1 cut(s) 729
DraIII CACNNNGTG 1 cut(s) 746
EaeI YGGCCR 3 cut(s) 97, 840, 1166
Eco130I CCWWGG 2 cut(s) 340, 631
Eco47I GGWCC 1 cut(s) 215
EcoRI GAATTC 2 cut(s) 451, 696
EcoRII CCWGG 1 cut(s) 735
EcoT14I CCWWGG 2 cut(s) 340, 631
ErhI CCWWGG 2 cut(s) 340, 631
FaeI CATG 7 cut(s) 235, 264, 426, 436, 489, 601, 704
FaqI GGGAC 1 cut(s) 87
FatI CATG 7 cut(s) 231, 260, 422, 432, 485, 597, 700
Fnu4HI GCNGC 4 cut(s) 324, 591, 975, 1290
FokI GGATG 2 cut(s) 80, 853
Fsp4HI GCNGC 4 cut(s) 324, 591, 975, 1290
FspBI CTAG 1 cut(s) 1286
GlaI GCGC 1 cut(s) 380
GluI GCNGC 4 cut(s) 324, 591, 975, 1290
GsaI CCCAGC 2 cut(s) 47, 208
GsuI CTGGAG 2 cut(s) 702, 1093
HaeIII GGCC 4 cut(s) 99, 188, 842, 1168
HapII CCGG 3 cut(s) 290, 491, 1308
HhaI GCGC 1 cut(s) 381
Hin1II CATG 7 cut(s) 235, 264, 426, 436, 489, 601, 704
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HindIII AAGCTT 1 cut(s) 1049
HinfI GANTC 2 cut(s) 396, 706
HpaII CCGG 3 cut(s) 290, 491, 1308
HphI GGTGA 2 cut(s) 391, 758
Hpy166II GTNNAC 3 cut(s) 347, 385, 496
Hpy188I TCNGA 2 cut(s) 395, 820
Hpy188III TCNNGA 4 cut(s) 284, 1072, 1345, 1356
Hpy8I GTNNAC 3 cut(s) 347, 385, 496
HpyAV CCTTC 1 cut(s) 661
HpyCH4III ACNGT 3 cut(s) 586, 802, 1102
HpyCH4V TGCA 7 cut(s) 131, 440, 463, 593, 650, 1094, 1319
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 574, 647, 1048, 1286
HpyF3I CTNAG 5 cut(s) 58, 195, 241, 613, 1256
Hsp92II CATG 7 cut(s) 235, 264, 426, 436, 489, 601, 704
HspAI GCGC 1 cut(s) 379
Kzo9I GATC 1 cut(s) 729
LmnI GCTCC 3 cut(s) 80, 923, 1227
Lsp1109I GCAGC 4 cut(s) 310, 577, 986, 1301
LweI GCATC 2 cut(s) 43, 534
MaeI CTAG 1 cut(s) 1286
MaeIII GTNAC 7 cut(s) 244, 284, 292, 703, 746, 862, 1087
MalI GATC 1 cut(s) 731
MboI GATC 1 cut(s) 729
MboII GAAGA 7 cut(s) 257, 724, 847, 865, 1108, 1117, 1225
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 2 cut(s) 139, 1232
MlsI TGGCCA 1 cut(s) 1168
MluNI TGGCCA 1 cut(s) 1168
MlyI GAGTC 1 cut(s) 700
MmeI TCCRAC 3 cut(s) 63, 901, 1120
MnlI CCTC 7 cut(s) 65, 67, 720, 787, 791, 1068, 1256
Mox20I TGGCCA 1 cut(s) 1168
MroXI GAANNNNTTC 1 cut(s) 389
MscI TGGCCA 1 cut(s) 1168
MseI TTAA 3 cut(s) 351, 804, 1053
MslI CAYNNNNRTG 2 cut(s) 342, 468
Msp20I TGGCCA 1 cut(s) 1168
MspA1I CMGCKG 1 cut(s) 1292
MspCI CTTAAG 1 cut(s) 350
MspI CCGG 3 cut(s) 290, 491, 1308
MspR9I CCNGG 3 cut(s) 290, 491, 737
Mva1269I GAATGC 2 cut(s) 926, 956
MvaI CCWGG 1 cut(s) 737
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 5 cut(s) 53, 574, 647, 1048, 1286
NciI CCSGG 2 cut(s) 290, 491
NdeII GATC 1 cut(s) 729
NlaIII CATG 7 cut(s) 235, 264, 426, 436, 489, 601, 704
NlaIV GGNNCC 2 cut(s) 26, 76
NmeAIII GCCGAG 1 cut(s) 299
NmuCI GTSAC 7 cut(s) 244, 284, 292, 703, 746, 862, 1087
NspI RCATGY 1 cut(s) 426
NspV TTCGAA 1 cut(s) 331
OliI CACNNNNGTG 2 cut(s) 342, 468
PctI GAATGC 2 cut(s) 926, 956
PdmI GAANNNNTTC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 396
PflFI GACNNNGTC 1 cut(s) 290
PkrI GCNGC 4 cut(s) 325, 592, 976, 1291
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
Psp6I CCWGG 1 cut(s) 735
PspFI CCCAGC 2 cut(s) 43, 204
PspGI CCWGG 1 cut(s) 735
PspN4I GGNNCC 2 cut(s) 26, 76
PspPI GGNCC 2 cut(s) 186, 215
PstI CTGCAG 1 cut(s) 595
PsuI RGATCY 1 cut(s) 729
PsyI GACNNNGTC 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 1292
RsaI GTAC 4 cut(s) 356, 687, 1163, 1199
RsaNI GTAC 4 cut(s) 355, 686, 1162, 1198
RseI CAYNNNNRTG 2 cut(s) 342, 468
SaqAI TTAA 3 cut(s) 351, 804, 1053
SatI GCNGC 4 cut(s) 324, 591, 975, 1290
Sau3AI GATC 1 cut(s) 729
Sau96I GGNCC 2 cut(s) 186, 215
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 3 cut(s) 290, 491, 737
SduI GDGCHC 2 cut(s) 139, 1232
SfaNI GCATC 2 cut(s) 43, 534
SfcI CTRYAG 2 cut(s) 591, 774
SfuI TTCGAA 1 cut(s) 331
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 342, 468
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
SspMI CTAG 1 cut(s) 1286
StyD4I CCNGG 3 cut(s) 288, 489, 735
StyI CCWWGG 2 cut(s) 340, 631
TaaI ACNGT 3 cut(s) 586, 802, 1102
TaqI TCGA 4 cut(s) 331, 561, 1109, 1301
TatI WGTACW 2 cut(s) 354, 1197
TfiI GAWTC 1 cut(s) 396
Tru1I TTAA 3 cut(s) 351, 804, 1053
Tru9I TTAA 3 cut(s) 351, 804, 1053
TscAI CASTG 3 cut(s) 19, 301, 1105
TseFI GTSAC 7 cut(s) 244, 284, 292, 703, 746, 862, 1087
TseI GCWGC 4 cut(s) 323, 590, 974, 1289
Tsp45I GTSAC 7 cut(s) 244, 284, 292, 703, 746, 862, 1087
TspDTI ATGAA 6 cut(s) 23, 249, 680, 689, 848, 991
TspGWI ACGGA 1 cut(s) 859
TspRI CASTG 3 cut(s) 19, 301, 1105
Tth111I GACNNNGTC 1 cut(s) 290
Vha464I CTTAAG 1 cut(s) 350
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 4 cut(s) 451, 549, 556, 696
XceI RCATGY 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 389
XspI CTAG 1 cut(s) 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.