Prupe.1G491200_v2.0.a1

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001778
Physical Location & Seq
Reverse (-)
40855804 .. 40857792
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G491200.1

Sequence Viewer

Length: 1452 bp
ATGGGTGATCACACTACCACAAGGAACCTGCAGCTCCACCACAACCCAGTTTTTGATGTCTCACTCAATGTGCGAGGAGGCTCCAAGTACATTGATGACGATGGCCGTCCGAAACGAACTGGAACTGTTTGGACTTCAAGTGCTCACATAATTACTGCTGTGATTGGATCTGGGGTTCTATCCCTGGCTTGGGCCATTGCTCAGCTTGGATGGATTGCTGGTCCTGTTGTTATGGTCCTGTTCTCCTTCATCACTTACTACACTTCAACTCTTCTTGCTGCCTGCTACCGTGATCCGGTCACTGGAAAAAGAAACTATACTTATTCGGATGCTGTTCGATCCAACCTTGGTGAACTTCAGGTCAAATTCTGCGCATCTGTTCAGAACGTGGTCCTTATTGGAGTTACTATTGGCTACACTATAGCAGCAGCTATAAGCATGGTGGCAATAAAGAGGTCTAACTGCTACCACAAGAGTGGTGGAAAAAATCCATGTCATATTAACAACAATCCCTACATGATAGCTTTTGGTATCTCAGAAATTGTATTGTCTCAAATTCCAAACTTTGATAAGTTGTCCTGGCTCTCCATTGTTGCTGCAGTCATGTCCTTCACTTACTCAGGAATTGGATTAGCCCTTGGAATTGCTGAAGTTGCTGTAAATGGAAAAATCAAGGGAAATATCACTGGAATTACCATTGGCACTGTGAATCCAATTCAAAAGATGTGGAGGACCTTCCAAGCACTTGGTGACATAGCTTTTGCCTACTCTTACTCTATCATCCTCATTGAAATTCAGGACACAATTAAATCCCCACCATCTGAGTACAAGACAATGAAGAAGGCCACTCTATTTAGTTTGATAGTGACATCCATTTTCTACATTCTGTGTGGCTGCATGGGCTATGCTGCTTTTGGAGACTTGTCCCCTGGAAACCTCCTTACTGATAAAGGTTTCCACAACCCATTTTGGCTCATTGACATAGCCAATGCTGCCATAGTGATCCACCTTGTTGGTGCATACCAAGTGTTTGCACAACCCATTTTCGCACTCGTTGAGAAAACAGCTGCAGAATTTTTCCCAAATAGCCAATTTATCACAGAAGACATCAGAATCCCCATCCCTGGTTTCGGTGCCTACAACTTTAACCTCTTCAGATTTGTTTGGAGGACATTTTATGTAATCATAACCACTTTGATTTCCATGATCCTCCCATTCTTTAATGATGTGGTTGGATTTCTTGGGGCTTTGGGATATTGGCCACTCACAGTTTACTTCCCTGTGGAGATGTACATTGCTCAAAAGAAGGTACCAAAGTGGAGCACAAAATGGATTTGCCTCCAAATCCTAAGTCTTTCTGTCCTTGTAATAGCACTAGCTGCTGCAGCTGGCTCAGTTACTGGTGTGGTTCAAGACCTCAAGATCTACAAGCCCTTCAAGACCAGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

484

Amino Acids

53.02

Weight (kDa)

9.16

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 373
Acc36I ACCTGC 1 cut(s) 36
Acc65I GGTACC 1 cut(s) 1309
AccB1I GGYRCC 2 cut(s) 1133, 1309
AclWI GGATC 5 cut(s) 175, 287, 333, 997, 1201
AcoI YGGCCR 2 cut(s) 103, 1259
AcsI RAATTY 4 cut(s) 365, 555, 792, 1073
AcuI CTGAAG 3 cut(s) 341, 669, 1138
AdeI CACNNNGTG 1 cut(s) 749
AfaI GTAC 4 cut(s) 89, 827, 1292, 1311
AfiI CCNNNNNNNGG 6 cut(s) 189, 190, 295, 302, 1124, 1130
AgsI TTSAA 6 cut(s) 138, 267, 719, 791, 1412, 1438
AjnI CCWGG 4 cut(s) 183, 578, 928, 1123
AleI CACNNNNGTG 1 cut(s) 474
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
AluBI AGCT 8 cut(s) 34, 205, 431, 524, 758, 1067, 1379, 1388
AluI AGCT 8 cut(s) 34, 205, 431, 524, 758, 1067, 1379, 1388
Alw21I GWGCWC 2 cut(s) 145, 1325
Alw26I GTCTC 3 cut(s) 64, 555, 912
AlwI GGATC 5 cut(s) 175, 287, 333, 997, 1201
AlwNI CAGNNNCTG 1 cut(s) 1400
AoxI GGCC 4 cut(s) 103, 192, 843, 1259
ApoI RAATTY 4 cut(s) 365, 555, 792, 1073
ArsI GACNNNNNNTTYG 4 cut(s) 743, 775, 1336, 1368
Asp718I GGTACC 1 cut(s) 1309
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 5 cut(s) 192, 221, 235, 391, 732
AsuHPI GGTGA 3 cut(s) 17, 362, 761
AvaII GGWCC 4 cut(s) 221, 235, 391, 732
BaeI ACNNNNGTAYC 2 cut(s) 1301, 1334
BalI TGGCCA 1 cut(s) 1261
BanI GGYRCC 2 cut(s) 1133, 1309
BbsI GAAGAC 1 cut(s) 1110
Bbv12I GWGCWC 2 cut(s) 145, 1325
BccI CCATC 4 cut(s) 95, 204, 826, 1127
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 4 cut(s) 185, 580, 930, 1125
BclI TGATCA 1 cut(s) 7
BcoDI GTCTC 3 cut(s) 64, 555, 912
BfaI CTAG 1 cut(s) 1376
BfmI CTRYAG 5 cut(s) 29, 420, 597, 1068, 1383
BfuAI ACCTGC 1 cut(s) 36
BglII AGATCT 1 cut(s) 1422
BlpI GCTNAGC 1 cut(s) 201
Bme1390I CCNGG 4 cut(s) 185, 580, 930, 1125
Bme18I GGWCC 4 cut(s) 221, 235, 391, 732
BmgT120I GGNCC 5 cut(s) 192, 221, 235, 391, 732
BmiI GGNNCC 4 cut(s) 26, 82, 1135, 1311
BmrFI CCNGG 4 cut(s) 185, 580, 930, 1125
BmrI ACTGGG 1 cut(s) 41
BmsI GCATC 2 cut(s) 319, 383
BmuI ACTGGG 1 cut(s) 41
BpiI GAAGAC 1 cut(s) 1110
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 1403
BsaJI CCNNGG 5 cut(s) 183, 346, 637, 928, 1123
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 6 cut(s) 189, 190, 295, 302, 1124, 1130
Bse1I ACTGG 5 cut(s) 47, 124, 307, 691, 1405
Bse3DI GCAATG 2 cut(s) 195, 1293
BseBI CCWGG 4 cut(s) 185, 580, 930, 1125
BseDI CCNNGG 5 cut(s) 183, 346, 637, 928, 1123
BseGI GGATG 5 cut(s) 215, 334, 780, 869, 1119
BseLI CCNNNNNNNGG 6 cut(s) 189, 190, 295, 302, 1124, 1130
BseMI GCAATG 2 cut(s) 195, 1293
BseMII CTCAG 5 cut(s) 215, 549, 633, 813, 1407
BseNI ACTGG 5 cut(s) 47, 124, 307, 691, 1405
BseRI GAGGAG 1 cut(s) 90
BshFI GGCC 4 cut(s) 105, 194, 845, 1261
BshNI GGYRCC 2 cut(s) 1133, 1309
BsiHKAI GWGCWC 2 cut(s) 145, 1325
BsiSI CCGG 1 cut(s) 296
BslFI GGGAC 1 cut(s) 910
BslI CCNNNNNNNGG 6 cut(s) 189, 190, 295, 302, 1124, 1130
BsmAI GTCTC 3 cut(s) 64, 555, 912
BsmFI GGGAC 1 cut(s) 910
BsnI GGCC 4 cut(s) 105, 194, 845, 1261
Bsp1286I GDGCHC 2 cut(s) 145, 1325
Bsp1407I TGTACA 1 cut(s) 1290
Bsp143I GATC 7 cut(s) 7, 167, 292, 338, 1002, 1206, 1422
Bsp1720I GCTNAGC 1 cut(s) 201
BspANI GGCC 4 cut(s) 105, 194, 845, 1261
BspCNI CTCAG 5 cut(s) 214, 548, 632, 814, 1406
BspLI GGNNCC 4 cut(s) 26, 82, 1135, 1311
BspMAI CTGCAG 4 cut(s) 33, 601, 1072, 1387
BspMI ACCTGC 1 cut(s) 36
BspPI GGATC 5 cut(s) 175, 287, 333, 997, 1201
BspT107I GGYRCC 2 cut(s) 1133, 1309
BsrDI GCAATG 2 cut(s) 195, 1293
BsrGI TGTACA 1 cut(s) 1290
BsrI ACTGG 5 cut(s) 47, 124, 307, 691, 1405
BssECI CCNNGG 5 cut(s) 183, 346, 637, 928, 1123
BssMI GATC 7 cut(s) 7, 167, 292, 338, 1002, 1206, 1422
BssT1I CCWWGG 2 cut(s) 346, 637
Bst2UI CCWGG 4 cut(s) 185, 580, 930, 1125
Bst4CI ACNGT 4 cut(s) 127, 290, 706, 1270
Bst6I CTCTTC 2 cut(s) 276, 1157
BstAPI GCANNNNNTGC 1 cut(s) 1379
BstAUI TGTACA 1 cut(s) 1290
BstC8I GCNNGC 2 cut(s) 283, 1390
BstDEI CTNAG 6 cut(s) 201, 535, 619, 822, 1349, 1393
BstF5I GGATG 5 cut(s) 215, 334, 780, 869, 1119
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 7 cut(s) 10, 170, 295, 341, 1005, 1209, 1425
BstMAI GTCTC 3 cut(s) 64, 555, 912
BstMBI GATC 7 cut(s) 7, 167, 292, 338, 1002, 1206, 1422
BstMWI GCNNNNNNNGC 5 cut(s) 653, 900, 992, 1379, 1385
BstNI CCWGG 4 cut(s) 185, 580, 930, 1125
BstSCI CCNGG 4 cut(s) 183, 578, 928, 1123
BstSFI CTRYAG 5 cut(s) 29, 420, 597, 1068, 1383
BstV2I GAAGAC 1 cut(s) 1110
BstX2I RGATCY 2 cut(s) 167, 1422
BstXI CCANNNNNNTGG 3 cut(s) 476, 746, 1013
BstYI RGATCY 2 cut(s) 167, 1422
BsuRI GGCC 4 cut(s) 105, 194, 845, 1261
BtsCI GGATG 5 cut(s) 215, 334, 780, 869, 1119
BtsIMutI CAGTG 3 cut(s) 300, 684, 702
BveI ACCTGC 1 cut(s) 36
Cac8I GCNNGC 2 cut(s) 283, 1390
CaiI CAGNNNCTG 1 cut(s) 1400
CfoI GCGC 1 cut(s) 374
Cfr13I GGNCC 5 cut(s) 192, 221, 235, 391, 732
Csp6I GTAC 4 cut(s) 88, 826, 1291, 1310
CspCI CAANNNNNGTGG 4 cut(s) 460, 495, 707, 742
CviAII CATG 6 cut(s) 439, 492, 517, 604, 898, 1204
CviQI GTAC 4 cut(s) 88, 826, 1291, 1310
DdeI CTNAG 6 cut(s) 201, 535, 619, 822, 1349, 1393
DpnI GATC 7 cut(s) 9, 169, 294, 340, 1004, 1208, 1424
DpnII GATC 7 cut(s) 7, 167, 292, 338, 1002, 1206, 1422
DraIII CACNNNGTG 1 cut(s) 749
EaeI YGGCCR 2 cut(s) 103, 1259
Eam1104I CTCTTC 2 cut(s) 276, 1157
EarI CTCTTC 2 cut(s) 276, 1157
Eco130I CCWWGG 2 cut(s) 346, 637
Eco47I GGWCC 4 cut(s) 221, 235, 391, 732
Eco57I CTGAAG 3 cut(s) 341, 669, 1138
EcoO109I RGGNCCY 1 cut(s) 732
EcoRII CCWGG 4 cut(s) 183, 578, 928, 1123
EcoT14I CCWWGG 2 cut(s) 346, 637
ErhI CCWWGG 2 cut(s) 346, 637
FaeI CATG 6 cut(s) 442, 495, 520, 607, 901, 1207
FaqI GGGAC 1 cut(s) 910
FatI CATG 6 cut(s) 438, 491, 516, 603, 897, 1203
FbaI TGATCA 1 cut(s) 7
FokI GGATG 5 cut(s) 222, 341, 767, 856, 1106
FspBI CTAG 1 cut(s) 1376
FspI TGCGCA 1 cut(s) 373
GlaI GCGC 1 cut(s) 373
HaeIII GGCC 4 cut(s) 105, 194, 845, 1261
HapII CCGG 1 cut(s) 296
HhaI GCGC 1 cut(s) 374
Hin1II CATG 6 cut(s) 442, 495, 520, 607, 901, 1207
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HinfI GANTC 2 cut(s) 709, 1113
HpaII CCGG 1 cut(s) 296
HphI GGTGA 3 cut(s) 17, 362, 761
Hpy166II GTNNAC 2 cut(s) 353, 1273
Hpy188I TCNGA 7 cut(s) 111, 328, 384, 538, 823, 1112, 1157
Hpy188III TCNNGA 5 cut(s) 621, 797, 1412, 1420, 1438
Hpy8I GTNNAC 2 cut(s) 353, 1273
HpyAV CCTTC 6 cut(s) 256, 619, 745, 835, 1300, 1444
HpyCH4III ACNGT 4 cut(s) 127, 290, 706, 1270
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 7 cut(s) 31, 599, 897, 1019, 1034, 1070, 1385
HpyF10VI GCNNNNNNNGC 5 cut(s) 653, 900, 992, 1379, 1385
HpyF3I CTNAG 6 cut(s) 201, 535, 619, 822, 1349, 1393
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 6 cut(s) 442, 495, 520, 607, 901, 1207
HspAI GCGC 1 cut(s) 372
KpnI GGTACC 1 cut(s) 1313
Ksp22I TGATCA 1 cut(s) 7
Kzo9I GATC 7 cut(s) 7, 167, 292, 338, 1002, 1206, 1422
LmnI GCTCC 3 cut(s) 39, 86, 1320
LweI GCATC 2 cut(s) 319, 383
MaeI CTAG 1 cut(s) 1376
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 5 cut(s) 298, 403, 749, 865, 1396
MalI GATC 7 cut(s) 9, 169, 294, 340, 1004, 1208, 1424
MboI GATC 7 cut(s) 7, 167, 292, 338, 1002, 1206, 1422
MboII GAAGA 4 cut(s) 263, 850, 1115, 1144
MflI RGATCY 2 cut(s) 167, 1422
MhlI GDGCHC 2 cut(s) 145, 1325
MlsI TGGCCA 1 cut(s) 1261
MluNI TGGCCA 1 cut(s) 1261
MmeI TCCRAC 2 cut(s) 366, 1213
Mox20I TGGCCA 1 cut(s) 1261
MscI TGGCCA 1 cut(s) 1261
MseI TTAA 4 cut(s) 501, 807, 1146, 1221
MslI CAYNNNNRTG 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 1261
MspA1I CMGCKG 2 cut(s) 1067, 1388
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 4 cut(s) 185, 580, 930, 1125
MvaI CCWGG 4 cut(s) 185, 580, 930, 1125
MwoI GCNNNNNNNGC 5 cut(s) 653, 900, 992, 1379, 1385
NdeII GATC 7 cut(s) 7, 167, 292, 338, 1002, 1206, 1422
NlaIII CATG 6 cut(s) 442, 495, 520, 607, 901, 1207
NlaIV GGNNCC 4 cut(s) 26, 82, 1135, 1311
NmuCI GTSAC 3 cut(s) 298, 749, 865
NsbI TGCGCA 1 cut(s) 373
OliI CACNNNNGTG 1 cut(s) 474
PfeI GAWTC 2 cut(s) 709, 1113
PpuMI RGGWCCY 1 cut(s) 732
Psp5II RGGWCCY 1 cut(s) 732
Psp6I CCWGG 4 cut(s) 183, 578, 928, 1123
PspGI CCWGG 4 cut(s) 183, 578, 928, 1123
PspN4I GGNNCC 4 cut(s) 26, 82, 1135, 1311
PspPI GGNCC 5 cut(s) 192, 221, 235, 391, 732
PspPPI RGGWCCY 1 cut(s) 732
PstI CTGCAG 4 cut(s) 33, 601, 1072, 1387
PstNI CAGNNNCTG 1 cut(s) 1400
PsuI RGATCY 2 cut(s) 167, 1422
PvuII CAGCTG 2 cut(s) 1067, 1388
RsaI GTAC 4 cut(s) 89, 827, 1292, 1311
RsaNI GTAC 4 cut(s) 88, 826, 1291, 1310
RseI CAYNNNNRTG 1 cut(s) 474
SaqAI TTAA 4 cut(s) 501, 807, 1146, 1221
Sau3AI GATC 7 cut(s) 7, 167, 292, 338, 1002, 1206, 1422
Sau96I GGNCC 5 cut(s) 192, 221, 235, 391, 732
ScrFI CCNGG 4 cut(s) 185, 580, 930, 1125
SduI GDGCHC 2 cut(s) 145, 1325
SfaNI GCATC 2 cut(s) 319, 383
SfcI CTRYAG 5 cut(s) 29, 420, 597, 1068, 1383
SinI GGWCC 4 cut(s) 221, 235, 391, 732
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 1418
SmoI CTYRAG 1 cut(s) 1418
SspMI CTAG 1 cut(s) 1376
StyD4I CCNGG 4 cut(s) 183, 578, 928, 1123
StyI CCWWGG 2 cut(s) 346, 637
TaaI ACNGT 4 cut(s) 127, 290, 706, 1270
TaiI ACGT 1 cut(s) 390
TaqI TCGA 1 cut(s) 337
TatI WGTACW 3 cut(s) 87, 825, 1290
TfiI GAWTC 2 cut(s) 709, 1113
Tru1I TTAA 4 cut(s) 501, 807, 1146, 1221
Tru9I TTAA 4 cut(s) 501, 807, 1146, 1221
TscAI CASTG 3 cut(s) 307, 691, 709
TseFI GTSAC 3 cut(s) 298, 749, 865
Tsp45I GTSAC 3 cut(s) 298, 749, 865
TspDTI ATGAA 2 cut(s) 238, 851
TspRI CASTG 3 cut(s) 307, 691, 709
VpaK11BI GGWCC 4 cut(s) 221, 235, 391, 732
XapI RAATTY 4 cut(s) 365, 555, 792, 1073
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XspI CTAG 1 cut(s) 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.