FvH4_5g12780

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001778
Physical Location & Seq
Forward (+)
7234517 .. 7237342
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12780.t1

Sequence Viewer

Length: 1434 bp
ATGGGGGACAACACTGCTTCAAGAAACCACATCCATTCCAACCCAGCTTTTGATGCCTCAGTTATGGAAGGAGGGTTTAAGTTCTTTGATGATGATGGCCATCCCAAAAGGACTGGAAATGTTATGACTGCAAGTGCTCACATAATTACTGCTGTGATTGGTTCTGGGGTTTTGTCGCTGGCTTGGGCCATTGCTCAGCTTGGCTGGGTTGTTGGTCCTATTGTGATGGTAATGTTTTCGATAGTGACTTACTACACTTCATGTCTTCTCGTTGCCTGCTATCGTGATCCGGTCACTGGAAAAAGAAACTCTACTTACTCGGCTGCTGTTCGGAACCACCTTGGTGGATTTATGTACAAAGTTCTTGGAGTTGTTCAACGTGTCAATCTTTACGGAAGCACCATTGGCTACACCATAACAGCATGTATAAGCATGGTTGCAATACGAAGGAATGCCTGCGTGCATAAGCATGGTGAGGGAAAACCATGCCTGATAAACAGCAATCCCTATATGATTGCTTTTGGAGTCGTAGAAATTATATTATCTCAAATTCCAAATTTTGACAAGTTGTCGTGGCTATCTACTGTTGCTGCAATCATGTCATTTACATACTCAGGGATCGGACTTGCCATTGGAATTGCTAAAGTTGCAGAAACTGGAACAATCCAGGGAAGTCTTACTGGAGTAACCATTGGAGTTCAAGTGACTCGAGCCAAAAAGATATGGAGGATCTTCCAAGCACTTGGTGACATAGCTTTTGCTTACGCATTTGCTATCGTCCTCATTGAAATTGAGGACACGGTTAAATCTCCACCATCAGAAGCCAAGACAATGAAGACAGCCAGTAAAATAAGTTTATCAGTCACATCCATTTTCTACATTTTGTGTGGTTGCTTCGGTTATGCTGCTTTCGGAGATTTAGCCCCCGGAAACATCCTGACCGGTGCAGGGTTCTTTAACCCATCTTGGCTCATTGACATAGCCAATGCTGCCATAGTTATCCATCTTATTGGAGCATACCAAGTCTTCAGCCAGCCCTTTTACGCATTCGTTGAGAAAAAAGCAGCACAGCTTTTCCCTGATAACCACTTCATCACAAAAGACATCAAAATCATGTCCTACAAGCTTAACCTTTTTAGAGTGGTGTGGAGGACACTATATGTGATTTCAACCACTTTGTTATCGATGATCTTCCCGTTCTTCAATGGCGTGGTTGGATTTCTTGGGGCTTTGGGGTACTGGCCAATGTCAATCTACTTCCCTGTGGCGATGTACATTGCTCAAAAGAAGATACCAAAATGGAGCACAAGATGGATTTTCCTCCAAGTTCTATGTCTTTCTGCGCTAGTTATAGCTTTACTAGCTGCTGTTGGCTCGATTGTTGGTGTGGTGGAAAGTCTCAAAGATTACAAGCCATTCATGACCAGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

478

Amino Acids

51.99

Weight (kDa)

9.32

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 38 - 464 1.5e-109 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 281, 626, 737
AcoI YGGCCR 2 cut(s) 97, 1241
AcsI RAATTY 2 cut(s) 549, 556
AcuI CTGAAG 1 cut(s) 1012
AdeI CACNNNGTG 1 cut(s) 746
AfaI GTAC 3 cut(s) 356, 1238, 1274
AfiI CCNNNNNNNGG 2 cut(s) 296, 948
AflIII ACRYGT 1 cut(s) 379
AgeI ACCGGT 1 cut(s) 941
AgsI TTSAA 6 cut(s) 21, 377, 701, 788, 1170, 1204
AhdI GACNNNNNGTC 1 cut(s) 568
AjnI CCWGG 1 cut(s) 666
AleI CACNNNNGTG 1 cut(s) 342
AluBI AGCT 7 cut(s) 47, 199, 755, 1072, 1126, 1355, 1364
AluI AGCT 7 cut(s) 47, 199, 755, 1072, 1126, 1355, 1364
Alw21I GWGCWC 2 cut(s) 139, 1307
Alw26I GTCTC 1 cut(s) 1403
AlwI GGATC 3 cut(s) 281, 626, 737
AlwNI CAGNNNCTG 1 cut(s) 656
Ama87I CYCGRG 1 cut(s) 708
AoxI GGCC 3 cut(s) 97, 186, 1241
ApeKI GCWGC 6 cut(s) 323, 590, 905, 989, 1064, 1364
ApoI RAATTY 2 cut(s) 549, 556
ArsI GACNNNNNNTTYG 2 cut(s) 740, 772
AsiGI ACCGGT 1 cut(s) 941
AspLEI GCGC 1 cut(s) 1345
AspS9I GGNCC 2 cut(s) 186, 215
AsuC2I CCSGG 1 cut(s) 927
AsuHPI GGTGA 2 cut(s) 485, 758
AvaI CYCGRG 1 cut(s) 708
AvaII GGWCC 1 cut(s) 215
BalI TGGCCA 2 cut(s) 99, 1243
BbsI GAAGAC 3 cut(s) 257, 842, 1018
Bbv12I GWGCWC 2 cut(s) 139, 1307
BbvI GCAGC 6 cut(s) 310, 577, 892, 976, 1076, 1351
BccI CCATC 7 cut(s) 89, 108, 220, 823, 970, 1011, 1305
BciT130I CCWGG 1 cut(s) 668
BcnI CCSGG 1 cut(s) 927
BcoDI GTCTC 1 cut(s) 1403
BfaI CTAG 2 cut(s) 1346, 1361
BisI GCNGC 6 cut(s) 324, 591, 906, 990, 1065, 1365
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 6 cut(s) 325, 592, 907, 991, 1066, 1366
Bme1390I CCNGG 2 cut(s) 668, 927
Bme18I GGWCC 1 cut(s) 215
BmeRI GACNNNNNGTC 1 cut(s) 568
BmeT110I CYCGRG 1 cut(s) 708
BmgT120I GGNCC 2 cut(s) 186, 215
BmiI GGNNCC 1 cut(s) 335
BmrFI CCNGG 2 cut(s) 668, 927
BmsI GCATC 1 cut(s) 43
BpiI GAAGAC 3 cut(s) 257, 842, 1018
BpmI CTGGAG 1 cut(s) 702
Bpu1102I GCTNAGC 1 cut(s) 195
BpuMI CCSGG 1 cut(s) 927
Bsa29I ATCGAT 1 cut(s) 1184
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 340, 667, 925
BsaWI WCCGGW 2 cut(s) 289, 941
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 948
Bse118I RCCGGY 1 cut(s) 941
Bse1I ACTGG 6 cut(s) 118, 301, 661, 685, 843, 1244
Bse3DI GCAATG 2 cut(s) 189, 1275
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 1 cut(s) 668
BseCI ATCGAT 1 cut(s) 1184
BseDI CCNNGG 3 cut(s) 340, 667, 925
BseGI GGATG 4 cut(s) 30, 100, 866, 933
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 2 cut(s) 296, 948
BseMI GCAATG 2 cut(s) 189, 1275
BseMII CTCAG 3 cut(s) 72, 209, 627
BseNI ACTGG 6 cut(s) 118, 301, 661, 685, 843, 1244
BseXI GCAGC 6 cut(s) 310, 577, 892, 976, 1076, 1351
BseYI CCCAGC 2 cut(s) 43, 204
BsgI GTGCAG 1 cut(s) 966
BshFI GGCC 3 cut(s) 99, 188, 1243
BshTI ACCGGT 1 cut(s) 941
BshVI ATCGAT 1 cut(s) 1184
BsiHKAI GWGCWC 2 cut(s) 139, 1307
BsiHKCI CYCGRG 1 cut(s) 708
BsiSI CCGG 3 cut(s) 290, 927, 942
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 2 cut(s) 296, 948
BsmAI GTCTC 1 cut(s) 1403
BsmFI GGGAC 1 cut(s) 20
BsmI GAATGC 2 cut(s) 457, 1046
BsnI GGCC 3 cut(s) 99, 188, 1243
BsoBI CYCGRG 1 cut(s) 708
Bsp1286I GDGCHC 2 cut(s) 139, 1307
Bsp1407I TGTACA 2 cut(s) 354, 1272
Bsp143I GATC 4 cut(s) 286, 618, 729, 1188
Bsp1720I GCTNAGC 1 cut(s) 195
BspANI GGCC 3 cut(s) 99, 188, 1243
BspCNI CTCAG 3 cut(s) 71, 208, 626
BspDI ATCGAT 1 cut(s) 1184
BspHI TCATGA 1 cut(s) 1419
BspLI GGNNCC 1 cut(s) 335
BspPI GGATC 3 cut(s) 281, 626, 737
BsrDI GCAATG 2 cut(s) 189, 1275
BsrFI RCCGGY 1 cut(s) 941
BsrGI TGTACA 2 cut(s) 354, 1272
BsrI ACTGG 6 cut(s) 118, 301, 661, 685, 843, 1244
BssAI RCCGGY 1 cut(s) 941
BssECI CCNNGG 3 cut(s) 340, 667, 925
BssMI GATC 4 cut(s) 286, 618, 729, 1188
BssT1I CCWWGG 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 668
Bst4CI ACNGT 2 cut(s) 586, 802
BstAUI TGTACA 2 cut(s) 354, 1272
BstC8I GCNNGC 5 cut(s) 180, 277, 457, 461, 1034
BstDEI CTNAG 3 cut(s) 58, 195, 613
BstF5I GGATG 4 cut(s) 30, 100, 866, 933
BstHHI GCGC 1 cut(s) 1345
BstKTI GATC 4 cut(s) 289, 621, 732, 1191
BstMAI GTCTC 1 cut(s) 1403
BstMBI GATC 4 cut(s) 286, 618, 729, 1188
BstMWI GCNNNNNNNGC 5 cut(s) 53, 405, 647, 989, 1361
BstNI CCWGG 1 cut(s) 668
BstNSI RCATGY 1 cut(s) 426
BstSCI CCNGG 2 cut(s) 666, 925
BstV1I GCAGC 6 cut(s) 310, 577, 892, 976, 1076, 1351
BstV2I GAAGAC 3 cut(s) 257, 842, 1018
BstX2I RGATCY 1 cut(s) 729
BstXI CCANNNNNNTGG 3 cut(s) 344, 743, 1010
BstYI RGATCY 1 cut(s) 729
Bsu15I ATCGAT 1 cut(s) 1184
BsuRI GGCC 3 cut(s) 99, 188, 1243
BsuTUI ATCGAT 1 cut(s) 1184
BtgZI GCGATG 1 cut(s) 1283
BtsCI GGATG 4 cut(s) 30, 100, 866, 933
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 2 cut(s) 12, 294
Cac8I GCNNGC 5 cut(s) 180, 277, 457, 461, 1034
CaiI CAGNNNCTG 1 cut(s) 656
CciI TCATGA 1 cut(s) 1419
CfoI GCGC 1 cut(s) 1345
Cfr10I RCCGGY 1 cut(s) 941
Cfr13I GGNCC 2 cut(s) 186, 215
ClaI ATCGAT 1 cut(s) 1184
Csp6I GTAC 3 cut(s) 355, 1237, 1273
CspAI ACCGGT 1 cut(s) 941
CviAII CATG 8 cut(s) 261, 423, 433, 470, 486, 598, 1114, 1420
CviQI GTAC 3 cut(s) 355, 1237, 1273
DdeI CTNAG 3 cut(s) 58, 195, 613
DpnI GATC 4 cut(s) 288, 620, 731, 1190
DpnII GATC 4 cut(s) 286, 618, 729, 1188
DraIII CACNNNGTG 1 cut(s) 746
DriI GACNNNNNGTC 1 cut(s) 568
EaeI YGGCCR 2 cut(s) 97, 1241
Eam1105I GACNNNNNGTC 1 cut(s) 568
Eco130I CCWWGG 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 1012
Eco88I CYCGRG 1 cut(s) 708
EcoRII CCWGG 1 cut(s) 666
EcoT14I CCWWGG 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 8 cut(s) 264, 426, 436, 473, 489, 601, 1117, 1423
FaqI GGGAC 1 cut(s) 20
FatI CATG 8 cut(s) 260, 422, 432, 469, 485, 597, 1113, 1419
Fnu4HI GCNGC 6 cut(s) 324, 591, 906, 990, 1065, 1365
FokI GGATG 4 cut(s) 17, 87, 853, 920
Fsp4HI GCNGC 6 cut(s) 324, 591, 906, 990, 1065, 1365
FspBI CTAG 2 cut(s) 1346, 1361
GlaI GCGC 1 cut(s) 1344
GluI GCNGC 6 cut(s) 324, 591, 906, 990, 1065, 1365
GsaI CCCAGC 2 cut(s) 47, 208
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 3 cut(s) 99, 188, 1243
HapII CCGG 3 cut(s) 290, 927, 942
HhaI GCGC 1 cut(s) 1345
Hin1II CATG 8 cut(s) 264, 426, 436, 473, 489, 601, 1117, 1423
Hin6I GCGC 1 cut(s) 1343
HinP1I GCGC 1 cut(s) 1343
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 2 cut(s) 525, 706
HpaII CCGG 3 cut(s) 290, 927, 942
HphI GGTGA 2 cut(s) 485, 758
Hpy188I TCNGA 4 cut(s) 333, 623, 820, 914
Hpy188III TCNNGA 4 cut(s) 21, 284, 937, 1420
HpyAV CCTTC 2 cut(s) 62, 441
HpyCH4III ACNGT 2 cut(s) 586, 802
HpyCH4IV ACGT 1 cut(s) 379
HpyCH4V TGCA 6 cut(s) 131, 440, 463, 593, 650, 947
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 405, 647, 989, 1361
HpyF3I CTNAG 3 cut(s) 58, 195, 613
HpySE526I ACGT 1 cut(s) 379
Hsp92II CATG 8 cut(s) 264, 426, 436, 473, 489, 601, 1117, 1423
HspAI GCGC 1 cut(s) 1343
Kzo9I GATC 4 cut(s) 286, 618, 729, 1188
LmnI GCTCC 2 cut(s) 1013, 1302
Lsp1109I GCAGC 6 cut(s) 310, 577, 892, 976, 1076, 1351
LweI GCATC 1 cut(s) 43
MaeI CTAG 2 cut(s) 1346, 1361
MaeII ACGT 1 cut(s) 379
MaeIII GTNAC 6 cut(s) 244, 292, 685, 703, 746, 862
MalI GATC 4 cut(s) 288, 620, 731, 1190
MboI GATC 4 cut(s) 286, 618, 729, 1188
MboII GAAGA 7 cut(s) 257, 724, 847, 1018, 1183, 1192, 1300
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 2 cut(s) 139, 1307
MlsI TGGCCA 2 cut(s) 99, 1243
MluCI AATT 6 cut(s) 144, 534, 549, 556, 636, 789
MluNI TGGCCA 2 cut(s) 99, 1243
MlyI GAGTC 2 cut(s) 534, 700
MmeI TCCRAC 2 cut(s) 63, 1195
MnlI CCTC 8 cut(s) 65, 67, 469, 720, 787, 791, 1143, 1331
Mox20I TGGCCA 2 cut(s) 99, 1243
MscI TGGCCA 2 cut(s) 99, 1243
MseI TTAA 4 cut(s) 78, 804, 957, 1128
MslI CAYNNNNRTG 2 cut(s) 342, 468
Msp20I TGGCCA 2 cut(s) 99, 1243
MspI CCGG 3 cut(s) 290, 927, 942
MspR9I CCNGG 2 cut(s) 668, 927
Mva1269I GAATGC 2 cut(s) 457, 1046
MvaI CCWGG 1 cut(s) 668
MwoI GCNNNNNNNGC 5 cut(s) 53, 405, 647, 989, 1361
NciI CCSGG 1 cut(s) 927
NdeII GATC 4 cut(s) 286, 618, 729, 1188
NlaIII CATG 8 cut(s) 264, 426, 436, 473, 489, 601, 1117, 1423
NlaIV GGNNCC 1 cut(s) 335
NmeAIII GCCGAG 1 cut(s) 299
NmuCI GTSAC 5 cut(s) 244, 292, 703, 746, 862
NspI RCATGY 1 cut(s) 426
OliI CACNNNNGTG 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 708
PagI TCATGA 1 cut(s) 1419
PctI GAATGC 2 cut(s) 457, 1046
PinAI ACCGGT 1 cut(s) 941
PkrI GCNGC 6 cut(s) 325, 592, 907, 991, 1066, 1366
PleI GAGTC 2 cut(s) 533, 700
PpsI GAGTC 2 cut(s) 533, 700
Psp6I CCWGG 1 cut(s) 666
PspFI CCCAGC 2 cut(s) 43, 204
PspGI CCWGG 1 cut(s) 666
PspN4I GGNNCC 1 cut(s) 335
PspPI GGNCC 2 cut(s) 186, 215
PspXI VCTCGAGB 1 cut(s) 708
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 729
RsaI GTAC 3 cut(s) 356, 1238, 1274
RsaNI GTAC 3 cut(s) 355, 1237, 1273
RseI CAYNNNNRTG 2 cut(s) 342, 468
SaqAI TTAA 4 cut(s) 78, 804, 957, 1128
SatI GCNGC 6 cut(s) 324, 591, 906, 990, 1065, 1365
Sau3AI GATC 4 cut(s) 286, 618, 729, 1188
Sau96I GGNCC 2 cut(s) 186, 215
SchI GAGTC 2 cut(s) 534, 700
ScrFI CCNGG 2 cut(s) 668, 927
SduI GDGCHC 2 cut(s) 139, 1307
SfaNI GCATC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 708
SinI GGWCC 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 708
SmiMI CAYNNNNRTG 2 cut(s) 342, 468
SmlI CTYRAG 1 cut(s) 708
SmoI CTYRAG 1 cut(s) 708
Sse9I AATT 6 cut(s) 144, 534, 549, 556, 636, 789
SspMI CTAG 2 cut(s) 1346, 1361
StyD4I CCNGG 2 cut(s) 666, 925
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 586, 802
TaiI ACGT 1 cut(s) 382
TaqI TCGA 4 cut(s) 239, 709, 1184, 1376
TasI AATT 6 cut(s) 144, 534, 549, 556, 636, 789
TatI WGTACW 2 cut(s) 354, 1272
Tru1I TTAA 4 cut(s) 78, 804, 957, 1128
Tru9I TTAA 4 cut(s) 78, 804, 957, 1128
TscAI CASTG 2 cut(s) 19, 301
TseFI GTSAC 5 cut(s) 244, 292, 703, 746, 862
TseI GCWGC 6 cut(s) 323, 590, 905, 989, 1064, 1364
Tsp45I GTSAC 5 cut(s) 244, 292, 703, 746, 862
TspDTI ATGAA 4 cut(s) 249, 848, 1081, 1408
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 2 cut(s) 19, 301
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 2 cut(s) 549, 556
XceI RCATGY 1 cut(s) 426
XhoI CTCGAG 1 cut(s) 708
XspI CTAG 2 cut(s) 1346, 1361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.