MD08G1158200.v1.1

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001778
Physical Location & Seq
Forward (+)
17724430 .. 17726888
2459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1158200.v1.1.491

Sequence Viewer

Length: 1503 bp
ATGGGTGAAAACATTGCCACAAGGAACCAGCTGCAGTTTCACAACAGCCCAGCTTTTGATGTCTCACTCGATGTGCAAGGAGGCTCCAAAATCGTCGATGAAGATGGCCGCCTCATAAGAACCGGAACTCTCTGGACGGCAAGTTCACACATAATTACTGCTGTGATTGGGTCTGGAGTTCTGTCCTTGACTTGGGCCATAGCTCAGCTTGGATGGATTGCTGGTCCTGTTGTGATGGTCATGTTCTCCTTCATCACCTACTACACTTCAACTCTTCTTGTTTCCTGCTATCGTGATCCCGTCACTGGAAAGAGAAGCTGCACTTACTCGGATGCTGTTCGATCCCACCTTGGTAAAGTTCAGGTCAAAATGTGTGGATTTATTCAGCAATTGAACCTTTTTGGAACTACTATTGGCTATACTGTAACGGCAGCTATAAGCATGGTGGCAATAAAAAGGTCTACTTGCTTCCACAATAGTGGTGGAAAAGATCCATGCCTTACCAGCAGCAATCCCTATATGCTAGCTTTTGGAATCTCGGAAATTATATTGTCTCAAATTCCAAACTTTGATAAGTTGTCTTGGCTCTCCATTGTTGCTGCATTCATGTCCTTCACTTACTCTGGAATTGGACTAGCCCTTGGAATTGCTAAAGTTGCTGGTACACATATCTATTACTGCTATGTTGAATGGAGAAATGTTTTCTCCGAAAATGGAAAAATCAAGGGAAGCATGTCCGGAATGAGCATTGGGGTTGTGACTCCAGCCCAAAAGATGTGGAGGACCTTCCAAGCACTTGGTGACATAGCTTTTGCCTTCTCTTACTCAATCATCCTCATTGAAATTCAGGACACAGTTAAATCCCCACCATCTGAAACCAAAACAATGAAGAAGGCCACTCAACTTAGTTTGGCAGTGACATCCATTTTCTACATTCTATGCGGCTGCATGGGCTACGCTGCTTTTGGAGACTTAGCTCCGGGAAACCTCCTTACTGACAAAGGCTTATCCAGCCCATTTTGGCTCATCGACATAGCCAATGCCGCCATTGTGATCCACCTTGTTGGTGCATACCAAGTTTTCGCCCAACCCATTTTCGCATTGGTCGAAAAAACGGCAGCAAAATTTTTTCCAGACAGCCAGTTCATCACAAAAGACATCAAAATAACAATCCCGCGTTTAGGTTCCTACAACCTTAACCTCTTCAGTTTAGTTTGGAGAACATTATTTGTGATTCTAACCACTGTGATCTCCATGATCCTCCCATTCTTCAACAATGTGGTTGGATTTCTCGGGGCTTTGGGGTATTGGCCACTCACAGTTTATTTCCCCGTGGAGATGTACATTGCTCAAAAGAAGATACCAAAGTGGAGCATGAGATGGATTTGCCTCGAAATCCTCAGTTTTTGTGTCCTTGCAATCGCCTTATGTGCTGCAGGTGGTTCAGTTACTGGTGTGGTTCATGATCTCAAGGTCTACAGGCCCTTCAAAACCAGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

501

Amino Acids

54.81

Weight (kDa)

9.1

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 41 - 486 1e-111 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1427
Acc36I ACCTGC 1 cut(s) 1427
AccI GTMKAC 2 cut(s) 461, 1476
AccII CGCG 1 cut(s) 1177
AccIII TCCGGA 1 cut(s) 737
AciI CCGC 4 cut(s) 109, 942, 1044, 1175
AclWI GGATC 5 cut(s) 290, 336, 485, 1048, 1252
AcoI YGGCCR 2 cut(s) 106, 1310
AcsI RAATTY 3 cut(s) 558, 843, 1124
AcuI CTGAAG 1 cut(s) 1189
AdeI CACNNNGTG 1 cut(s) 800
AfaI GTAC 2 cut(s) 664, 1343
AfiI CCNNNNNNNGG 3 cut(s) 192, 305, 1181
AgsI TTSAA 6 cut(s) 270, 394, 689, 842, 1273, 1489
AleI CACNNNNGTG 1 cut(s) 477
AloI GAACNNNNNNTCC 2 cut(s) 127, 159
AluBI AGCT 9 cut(s) 31, 53, 203, 208, 318, 434, 527, 809, 977
AluI AGCT 9 cut(s) 31, 53, 203, 208, 318, 434, 527, 809, 977
Alw26I GTCTC 3 cut(s) 67, 558, 963
AlwI GGATC 5 cut(s) 290, 336, 485, 1048, 1252
AlwNI CAGNNNCTG 1 cut(s) 1451
Ama87I CYCGRG 1 cut(s) 1292
Aor13HI TCCGGA 1 cut(s) 737
AoxI GGCC 5 cut(s) 106, 195, 894, 1310, 1481
ApeKI GCWGC 9 cut(s) 31, 318, 431, 507, 599, 945, 959, 1118, 1433
ApoI RAATTY 3 cut(s) 558, 843, 1124
ArsI GACNNNNNNTTYG 2 cut(s) 794, 826
AspS9I GGNCC 4 cut(s) 195, 224, 783, 1482
AsuC2I CCSGG 1 cut(s) 981
AsuHPI GGTGA 3 cut(s) 17, 247, 812
AsuNHI GCTAGC 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 1292
AvaII GGWCC 2 cut(s) 224, 783
BaeI ACNNNNGTAYC 2 cut(s) 1352, 1385
BalI TGGCCA 1 cut(s) 1312
BbvI GCAGC 9 cut(s) 18, 305, 443, 519, 586, 932, 946, 1130, 1420
BccI CCATC 5 cut(s) 98, 207, 229, 877, 1374
BceAI ACGGC 3 cut(s) 153, 444, 1131
BcnI CCSGG 1 cut(s) 981
BcoDI GTCTC 3 cut(s) 67, 558, 963
BfaI CTAG 2 cut(s) 524, 635
BfmI CTRYAG 3 cut(s) 32, 1434, 1477
BfuAI ACCTGC 1 cut(s) 1427
BlpI GCTNAGC 1 cut(s) 204
Bme1390I CCNGG 1 cut(s) 981
Bme18I GGWCC 2 cut(s) 224, 783
BmeT110I CYCGRG 1 cut(s) 1292
BmgT120I GGNCC 4 cut(s) 195, 224, 783, 1482
BmiI GGNNCC 3 cut(s) 26, 85, 1186
BmrFI CCNGG 1 cut(s) 981
BmsI GCATC 1 cut(s) 322
BmtI GCTAGC 1 cut(s) 527
BpmI CTGGAG 2 cut(s) 195, 747
Bpu1102I GCTNAGC 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 1454
BpuMI CCSGG 1 cut(s) 981
BsaJI CCNNGG 3 cut(s) 349, 640, 1332
BsaWI WCCGGW 2 cut(s) 122, 737
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 305, 1181
Bse1I ACTGG 3 cut(s) 310, 1141, 1456
Bse3DI GCAATG 2 cut(s) 12, 1344
BseAI TCCGGA 1 cut(s) 737
BseDI CCNNGG 3 cut(s) 349, 640, 1332
BseGI GGATG 4 cut(s) 218, 337, 831, 920
BseLI CCNNNNNNNGG 3 cut(s) 192, 305, 1181
BseMI GCAATG 2 cut(s) 12, 1344
BseMII CTCAG 2 cut(s) 218, 1414
BseNI ACTGG 3 cut(s) 310, 1141, 1456
BseXI GCAGC 9 cut(s) 18, 305, 443, 519, 586, 932, 946, 1130, 1420
BseYI CCCAGC 1 cut(s) 49
BsgI GTGCAG 1 cut(s) 304
Bsh1236I CGCG 1 cut(s) 1177
BshFI GGCC 5 cut(s) 108, 197, 896, 1312, 1483
BsiHKCI CYCGRG 1 cut(s) 1292
BsiSI CCGG 3 cut(s) 123, 738, 980
BslI CCNNNNNNNGG 3 cut(s) 192, 305, 1181
BsmAI GTCTC 3 cut(s) 67, 558, 963
BsmI GAATGC 1 cut(s) 602
BsnI GGCC 5 cut(s) 108, 197, 896, 1312, 1483
BsoBI CYCGRG 1 cut(s) 1292
Bsp13I TCCGGA 1 cut(s) 737
Bsp1407I TGTACA 1 cut(s) 1341
Bsp143I GATC 7 cut(s) 295, 341, 490, 1053, 1248, 1257, 1465
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 4 cut(s) 109, 942, 1044, 1175
BspANI GGCC 5 cut(s) 108, 197, 896, 1312, 1483
BspCNI CTCAG 2 cut(s) 217, 1413
BspEI TCCGGA 1 cut(s) 737
BspFNI CGCG 1 cut(s) 1177
BspHI TCATGA 1 cut(s) 1462
BspLI GGNNCC 3 cut(s) 26, 85, 1186
BspMAI CTGCAG 2 cut(s) 36, 1438
BspMI ACCTGC 1 cut(s) 1427
BspOI GCTAGC 1 cut(s) 527
BspPI GGATC 5 cut(s) 290, 336, 485, 1048, 1252
BsrDI GCAATG 2 cut(s) 12, 1344
BsrGI TGTACA 1 cut(s) 1341
BsrI ACTGG 3 cut(s) 310, 1141, 1456
BssECI CCNNGG 3 cut(s) 349, 640, 1332
BssMI GATC 7 cut(s) 295, 341, 490, 1053, 1248, 1257, 1465
BssT1I CCWWGG 2 cut(s) 349, 640
Bst4CI ACNGT 4 cut(s) 424, 856, 1246, 1321
Bst6I CTCTTC 2 cut(s) 279, 1208
BstAUI TGTACA 1 cut(s) 1341
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 4 cut(s) 204, 905, 973, 1400
BstDSI CCRYGG 1 cut(s) 1332
BstF5I GGATG 4 cut(s) 218, 337, 831, 920
BstFNI CGCG 1 cut(s) 1177
BstKTI GATC 7 cut(s) 298, 344, 493, 1056, 1251, 1260, 1468
BstMAI GTCTC 3 cut(s) 67, 558, 963
BstMBI GATC 7 cut(s) 295, 341, 490, 1053, 1248, 1257, 1465
BstMWI GCNNNNNNNGC 6 cut(s) 504, 656, 951, 1011, 1043, 1430
BstNSI RCATGY 1 cut(s) 736
BstSCI CCNGG 1 cut(s) 979
BstSFI CTRYAG 3 cut(s) 32, 1434, 1477
BstUI CGCG 1 cut(s) 1177
BstV1I GCAGC 9 cut(s) 18, 305, 443, 519, 586, 932, 946, 1130, 1420
BstX2I RGATCY 1 cut(s) 490
BstXI CCANNNNNNTGG 3 cut(s) 479, 797, 1064
BstYI RGATCY 1 cut(s) 490
BsuRI GGCC 5 cut(s) 108, 197, 896, 1312, 1483
BtgI CCRYGG 1 cut(s) 1332
BtsCI GGATG 4 cut(s) 218, 337, 831, 920
BtsI GCAGTG 1 cut(s) 921
BtsIMutI CAGTG 3 cut(s) 303, 921, 1242
BveI ACCTGC 1 cut(s) 1427
Cac8I GCNNGC 1 cut(s) 525
CaiI CAGNNNCTG 1 cut(s) 1451
CciI TCATGA 1 cut(s) 1462
Cfr13I GGNCC 4 cut(s) 195, 224, 783, 1482
Csp6I GTAC 2 cut(s) 663, 1342
CspCI CAANNNNNGTGG 6 cut(s) 355, 390, 463, 498, 758, 793
CviAII CATG 9 cut(s) 241, 442, 495, 607, 733, 949, 1255, 1375, 1463
CviQI GTAC 2 cut(s) 663, 1342
DdeI CTNAG 4 cut(s) 204, 905, 973, 1400
DpnI GATC 7 cut(s) 297, 343, 492, 1055, 1250, 1259, 1467
DpnII GATC 7 cut(s) 295, 341, 490, 1053, 1248, 1257, 1465
DraIII CACNNNGTG 1 cut(s) 800
EaeI YGGCCR 2 cut(s) 106, 1310
Eam1104I CTCTTC 2 cut(s) 279, 1208
EarI CTCTTC 2 cut(s) 279, 1208
Eco130I CCWWGG 2 cut(s) 349, 640
Eco47I GGWCC 2 cut(s) 224, 783
Eco57I CTGAAG 1 cut(s) 1189
Eco88I CYCGRG 1 cut(s) 1292
EcoO109I RGGNCCY 2 cut(s) 783, 1482
EcoT14I CCWWGG 2 cut(s) 349, 640
ErhI CCWWGG 2 cut(s) 349, 640
FaeI CATG 9 cut(s) 244, 445, 498, 610, 736, 952, 1258, 1378, 1466
FalI AAGNNNNNCTT 4 cut(s) 307, 339, 448, 480
FatI CATG 9 cut(s) 240, 441, 494, 606, 732, 948, 1254, 1374, 1462
FauI CCCGC 1 cut(s) 1182
FblI GTMKAC 2 cut(s) 461, 1476
FokI GGATG 4 cut(s) 225, 344, 818, 907
FspBI CTAG 2 cut(s) 524, 635
GsaI CCCAGC 1 cut(s) 53
GsuI CTGGAG 2 cut(s) 195, 747
HaeIII GGCC 5 cut(s) 108, 197, 896, 1312, 1483
HapII CCGG 3 cut(s) 123, 738, 980
Hin1II CATG 9 cut(s) 244, 445, 498, 610, 736, 952, 1258, 1378, 1466
HinfI GANTC 3 cut(s) 534, 760, 1234
HpaII CCGG 3 cut(s) 123, 738, 980
HphI GGTGA 3 cut(s) 17, 247, 812
Hpy166II GTNNAC 4 cut(s) 146, 462, 665, 1477
Hpy188I TCNGA 4 cut(s) 331, 541, 709, 874
Hpy188III TCNNGA 8 cut(s) 133, 174, 293, 624, 738, 848, 1133, 1463
Hpy8I GTNNAC 4 cut(s) 146, 462, 665, 1477
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 6 cut(s) 259, 622, 796, 826, 886, 1495
HpyCH4III ACNGT 4 cut(s) 424, 856, 1246, 1321
HpyCH4V TGCA 8 cut(s) 34, 76, 321, 602, 948, 1070, 1418, 1436
HpyF10VI GCNNNNNNNGC 6 cut(s) 504, 656, 951, 1011, 1043, 1430
HpyF3I CTNAG 4 cut(s) 204, 905, 973, 1400
Hsp92II CATG 9 cut(s) 244, 445, 498, 610, 736, 952, 1258, 1378, 1466
Kpn2I TCCGGA 1 cut(s) 737
Kzo9I GATC 7 cut(s) 295, 341, 490, 1053, 1248, 1257, 1465
LmnI GCTCC 3 cut(s) 89, 982, 1371
Lsp1109I GCAGC 9 cut(s) 18, 305, 443, 519, 586, 932, 946, 1130, 1420
LweI GCATC 1 cut(s) 322
MaeI CTAG 2 cut(s) 524, 635
MaeIII GTNAC 6 cut(s) 301, 424, 757, 800, 916, 1447
MalI GATC 7 cut(s) 297, 343, 492, 1055, 1250, 1259, 1467
MboI GATC 7 cut(s) 295, 341, 490, 1053, 1248, 1257, 1465
MboII GAAGA 6 cut(s) 113, 266, 901, 1195, 1261, 1369
MfeI CAATTG 1 cut(s) 389
MflI RGATCY 1 cut(s) 490
MlsI TGGCCA 1 cut(s) 1312
MluCI AATT 9 cut(s) 153, 389, 543, 558, 627, 645, 843, 1124, 1498
MluNI TGGCCA 1 cut(s) 1312
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 1 cut(s) 1264
MnlI CCTC 9 cut(s) 74, 122, 774, 845, 998, 1211, 1271, 1400, 1409
Mox20I TGGCCA 1 cut(s) 1312
MroI TCCGGA 1 cut(s) 737
MscI TGGCCA 1 cut(s) 1312
MseI TTAA 3 cut(s) 858, 1197, 1501
MslI CAYNNNNRTG 1 cut(s) 477
Msp20I TGGCCA 1 cut(s) 1312
MspA1I CMGCKG 1 cut(s) 31
MspI CCGG 3 cut(s) 123, 738, 980
MspR9I CCNGG 1 cut(s) 981
MunI CAATTG 1 cut(s) 389
Mva1269I GAATGC 1 cut(s) 602
MvnI CGCG 1 cut(s) 1177
MwoI GCNNNNNNNGC 6 cut(s) 504, 656, 951, 1011, 1043, 1430
NciI CCSGG 1 cut(s) 981
NdeII GATC 7 cut(s) 295, 341, 490, 1053, 1248, 1257, 1465
NheI GCTAGC 1 cut(s) 523
NlaIII CATG 9 cut(s) 244, 445, 498, 610, 736, 952, 1258, 1378, 1466
NlaIV GGNNCC 3 cut(s) 26, 85, 1186
NmuCI GTSAC 4 cut(s) 301, 757, 800, 916
NspI RCATGY 1 cut(s) 736
OliI CACNNNNGTG 1 cut(s) 477
PagI TCATGA 1 cut(s) 1462
PaqCI CACCTGC 1 cut(s) 1427
PcsI WCGNNNNNNNCGW 1 cut(s) 1104
PctI GAATGC 1 cut(s) 602
PfeI GAWTC 2 cut(s) 534, 1234
PfoI TCCNGGA 1 cut(s) 979
PleI GAGTC 1 cut(s) 754
PpsI GAGTC 1 cut(s) 754
PpuMI RGGWCCY 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 783
PspFI CCCAGC 1 cut(s) 49
PspN4I GGNNCC 3 cut(s) 26, 85, 1186
PspPI GGNCC 4 cut(s) 195, 224, 783, 1482
PspPPI RGGWCCY 1 cut(s) 783
PstI CTGCAG 2 cut(s) 36, 1438
PstNI CAGNNNCTG 1 cut(s) 1451
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 31
RsaI GTAC 2 cut(s) 664, 1343
RsaNI GTAC 2 cut(s) 663, 1342
RseI CAYNNNNRTG 1 cut(s) 477
SaqAI TTAA 3 cut(s) 858, 1197, 1501
Sau3AI GATC 7 cut(s) 295, 341, 490, 1053, 1248, 1257, 1465
Sau96I GGNCC 4 cut(s) 195, 224, 783, 1482
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 1 cut(s) 981
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 3 cut(s) 32, 1434, 1477
SinI GGWCC 2 cut(s) 224, 783
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 1469
SmoI CTYRAG 1 cut(s) 1469
Sse9I AATT 9 cut(s) 153, 389, 543, 558, 627, 645, 843, 1124, 1498
SsiI CCGC 4 cut(s) 109, 942, 1044, 1175
SspMI CTAG 2 cut(s) 524, 635
StyD4I CCNGG 1 cut(s) 979
StyI CCWWGG 2 cut(s) 349, 640
TaaI ACNGT 4 cut(s) 424, 856, 1246, 1321
TaqI TCGA 6 cut(s) 69, 96, 340, 1029, 1107, 1392
TasI AATT 9 cut(s) 153, 389, 543, 558, 627, 645, 843, 1124, 1498
TatI WGTACW 1 cut(s) 1341
TauI GCSGC 3 cut(s) 111, 945, 1046
TfiI GAWTC 2 cut(s) 534, 1234
Tru1I TTAA 3 cut(s) 858, 1197, 1501
Tru9I TTAA 3 cut(s) 858, 1197, 1501
TscAI CASTG 3 cut(s) 310, 921, 1249
TseFI GTSAC 4 cut(s) 301, 757, 800, 916
TseI GCWGC 9 cut(s) 31, 318, 431, 507, 599, 945, 959, 1118, 1433
Tsp45I GTSAC 4 cut(s) 301, 757, 800, 916
TspDTI ATGAA 6 cut(s) 114, 241, 595, 902, 1135, 1451
TspRI CASTG 3 cut(s) 310, 921, 1249
VpaK11BI GGWCC 2 cut(s) 224, 783
XapI RAATTY 3 cut(s) 558, 843, 1124
XceI RCATGY 1 cut(s) 736
XcmI CCANNNNNNNNNTGG 2 cut(s) 479, 1099
XmiI GTMKAC 2 cut(s) 461, 1476
XspI CTAG 2 cut(s) 524, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.