FvH4_2g27821

DNA topoisomerase 2-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003647
Physical Location & Seq
Reverse (-)
21979519 .. 21987017
7499 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27821.t2

Sequence Viewer

Length: 2934 bp
ATGCTCCAAACGCCGCCGTTTAAGGGCGCCAACGTCTTCATGTCGCGGAAGCTCGTCCCGCCGGAGATTTTCGACGCGCTTCACGACGCGCTCAAGCTCAACGGCGCCAACGTCGTCCTCTGCTGCGACCCCTCCCGCACCGGCCCCGACGACTTCCACGTCATCGCTTCCTCCGATCACGAGAAGTTTGAGGATCTCAGAGCCAAGGGCTGCCAACTGCTCGGTCCTCAATGCGTGTTTTCTTGCGCGAACGAGCACAGGTCGCTGCCTAAACAAGGCTTTACTTGCTGCCTTGCATTGGATGGTGTCAATGTACTTGCCTCTGGTTTTGAGGCAGATGAAAAGATTAAGATAAGGAACTTAATAACGTCAATGGGGGGAGTTTTACATACTAAAACATCTTTGGATGTTAGCTTTGTCATTGCAAAGGATGTTTTAGCTACGAAATACAAGTGGGCTTTATATACATTGAAGAAGCCCGTCCTTACTATAAATTGGTTATATCAATGCTGCAAAGAACATCGCATTGTTCCTCAAGAATCCTTTAGGGTTCTTCCGTTTTCTGGCTTGATGATCTCCGTAACCAGAATTCCAGCAGATGAACGGAAGAAGATTGAAAAACTTGTCATCGAAAATGGTGGACAGTATTCTGGTGAACTTACAAAGAAGTGCACACATTTGATTTGTGATGCTCCAGAAGGTGACAAGTACAAGGTTGCACAAAAGTGGGGTTATATTTATATTGTAACTCGGAAATGGTTTGATCAATCGATTGCTAGAAGAGCTTGTCTAAATGAGGAGTCCTTTCCAGTTCAAGGTGATTCTTTATCTTCAAAGAAGATTGTAAAGGGTTGCTTTGCTTCACAAAATAGCCAAAGCAATCGTAGTGGGTTCTTGCAAGCTGCACCGCCATCGGTGGTTGCAGATTCAAATTTTACAGCTTCTACTTGTGGTGGGATTGTAGATTCTGATTTAGAAGCTTCTGTTTCACAAAGCATGCTTCCAAAATCTTCTTGTGCCCCTGTATTTTGTAAAGAGGGGGATAGCAAAGTCCCTCCCTTGGAATCAAAATATGAGGTGACCCTTGAAGATTGTGTTGCTGATGATTCTGAGTCTGAAGAAAATGACCTGTACTTGTCAGAATGTAGAATATCACTTGTTGGTTTTGAAGCTTCTGAAATGCGTCGATTGGTTAATATTATTCGCAGAGGTGGGGGCACCCGTTATATGTCATTCAATGATAGGTTGACACACATAGTAGTTGGAACTCCTTCTGAAATTGAAAAGAAGGAGGTACGGGGATTTGCAGCTTGTGGTGTCATTCATGTGGTTAGAGCCACCTGGCTTGAAGATTGTGATCGCCAGAAGAAAGAGATACCTGTTCTCCAGCAGCATAGTGCCTGTGATATACTTCTTCCTAAAGGAGCACTGACTGGTACAAGTGGTATGATTCAAGGGCAAATTTCTACTATTCATCAGAGGATGCCTTCTGATCAGTTATGTGGGAATAGGAGTGTGGCAACTGGAATGGATTTATTGGAGAAAAATAGAAAAGAGGTATCAGAAATAAATAGGGACGATGACAGGTCCGTGAAAGCAGCAGTTAGATCATTCGACAAGAACAGACTACCTGTCATAAAGGATAAAAGTAAGGTCCAGTTACATGACAAAACAAGGGGCCAACAGAAGATACATCTGGAATCAAGTGTTCAAAATGGCAAACCTTCTTCTGTATTTAAAGGGAAACTTTTCCGCTTTTCAGATTCCTTTCCTGAAGACAGGAGAGGTGAAATCATTGAATGGGTAAATCAAGGGGGAGGATTGACAATTGATGCTCATCTAAAGCAGAAGGCTCACTTCACTATTGAATGTCATGGTGTGATACCCAGATCTGTGGACTCTACTCATACTACATATGTATCAAGTCACTGGATCCGATCATGTTTGGAGGATGGATACTTGCTGGATGTTAGTAATCACATTCTTTACACTCCTCTTCCCTGCCGGACTCCGTTGCCTGGATTCGAACACTTTCGGTTTTCTGTTTCACAATACGAAGAGAAAGATAGACTGTTACTACGAAATTTGTGTTTTACACTGGGAGCTAAATTTGTGGAAAAACTGAATAAGAAGGTTACCCATTTGTTATGCAAGTTTACCAGTGGAAGTAAGTATGAGGCTGCTTGTAAATGGGGAACACATCGAGTTACCTGTGACTGGATATATGAGTGCGTGAAGCAGAATAAAGTCATCGCTCTCGACCAATATTATCCTAAAGAAGTTACTGCTGAAGATCAAGAGGCAGGGTTGTGTACTACAAGTCAGTTTCCTACACAAGATGTTCAAATGATATCTGTGGGCAATCCATCTGAGTGTCCAAGTCAATCACAAGATCGGAGCTTCTCACCCTTTGAAGCCATTGGTCGTAGAAATGAGAATATCAGGGAAGAAGCTAAAGAATCAAGTTGCACTAAGAAGGCAAGGATTTTTGAAGATGTTGAACAAAGCGGTCTTCTTTCTTCAGGGATAAGTATTAGCAATCCTCCAAATACGACTGGAAACCAGAAAGCGAACACTTTTGGTGAAGTATCCCAAGCTGTTCCTGATGTAGCTTCTGCTATTGAGGACTTGTTGGAGCAGACAACCAAGATACATGACCAGAGTTCATCAGAGAGTATTTTTGCATCGGATTGTGCAATGCTTGGTCACGACCCTCCGCGTCCTCTCTCCCTGAGTGGACTATCGAAACACTGGTTAAACAGCGGCGGGAAAAAGGATGACACTAACGATCCGTCTGGAAAGCAAACTGCTGGATTGTATGATCAATTTAGCGAAACACAGACCGATTCTCAGGTTATTGGATATGAGGAAGATTTGTCAGGCCGGCAAATGCTTATAGACAGTTTTCGGACGCGAAGTAGCAGTAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000785 GO:0001932 GO:0001934 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009893 GO:0009987 GO:0010556 GO:0010562 GO:0010604 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022402 GO:0022607 GO:0030174 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031570 GO:0032268 GO:0032270 GO:0033036 GO:0033135 GO:0033138 GO:0033260 GO:0033365 GO:0033554 GO:0033674 GO:0034502 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0035561 GO:0035563 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044786 GO:0045786 GO:0045859 GO:0045860 GO:0045937 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051098 GO:0051099 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051641 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070727 GO:0071163 GO:0071165 GO:0071168 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 PTCB-BRCT

Protein Analysis

978

Amino Acids

108.69

Weight (kDa)

6.8

Isoelectric Point (pI)

52.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 184 - 258 1.1e-13 BRCA1 C Terminus (BRCT) domain
BRCT_2 PF16589 187 - 269 6.5e-06 BRCT domain
PTCB-BRCT PF12738 190 - 253 1.3e-22 twin BRCT domain
BRCT PF00533 612 - 682 3.8e-07 BRCA1 C Terminus (BRCT) domain
BRCT_2 PF16589 612 - 693 2.5e-06 BRCT domain
PTCB-BRCT PF12738 617 - 677 3.3e-18 twin BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccB1I GGYRCC 3 cut(s) 26, 104, 1217
AccII CGCG 6 cut(s) 46, 77, 89, 248, 2719, 2914
AclWI GGATC 4 cut(s) 201, 1927, 1940, 2783
AcsI RAATTY 5 cut(s) 588, 931, 1461, 2083, 2108
AcuI CTGAAG 4 cut(s) 1137, 1794, 2310, 2505
AcyI GRCGYC 2 cut(s) 27, 105
AfaI GTAC 6 cut(s) 315, 710, 1133, 1296, 1438, 2314
AfiI CCNNNNNNNGG 8 cut(s) 23, 275, 298, 563, 815, 1060, 2018, 2217
AhdI GACNNNNNGTC 1 cut(s) 1631
AjiI CACGTC 1 cut(s) 160
AjnI CCWGG 2 cut(s) 1340, 2017
AleI CACNNNNGTG 1 cut(s) 724
Alw21I GWGCWC 3 cut(s) 258, 674, 1429
Alw44I GTGCAC 1 cut(s) 670
AlwI GGATC 4 cut(s) 201, 1927, 1940, 2783
AoxI GGCC 3 cut(s) 142, 1678, 2881
ApaLI GTGCAC 1 cut(s) 670
ApoI RAATTY 5 cut(s) 588, 931, 1461, 2083, 2108
Asp700I GAANNNNTTC 3 cut(s) 1270, 1748, 2031
AspLEI GCGC 5 cut(s) 29, 79, 91, 107, 248
AspS9I GGNCC 5 cut(s) 143, 224, 1587, 1654, 1678
AsuHPI GGTGA 7 cut(s) 665, 713, 830, 1090, 1799, 2397, 2594
AsuII TTCGAA 1 cut(s) 2025
AvaII GGWCC 3 cut(s) 224, 1587, 1654
BaeGI GKGCMC 3 cut(s) 674, 1021, 1220
BaeI ACNNNNGTAYC 2 cut(s) 1902, 1935
BamHI GGATCC 1 cut(s) 1932
BanI GGYRCC 3 cut(s) 26, 104, 1217
BauI CACGAG 1 cut(s) 179
BbsI GAAGAC 3 cut(s) 28, 1782, 2504
Bbv12I GWGCWC 3 cut(s) 258, 674, 1429
BccI CCATC 4 cut(s) 296, 919, 1946, 2374
BceAI ACGGC 1 cut(s) 118
BcgI CGANNNNNNTGC 2 cut(s) 894, 928
BciT130I CCWGG 2 cut(s) 1342, 2019
BciVI GTATCC 2 cut(s) 1949, 2599
BclI TGATCA 3 cut(s) 763, 1492, 2821
BfaI CTAG 1 cut(s) 777
BfoI RGCGCY 2 cut(s) 30, 108
BfuI GTATCC 2 cut(s) 1949, 2599
BglII AGATCT 1 cut(s) 1889
Bme1390I CCNGG 2 cut(s) 1342, 2019
Bme18I GGWCC 3 cut(s) 224, 1587, 1654
BmeRI GACNNNNNGTC 1 cut(s) 1631
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 5 cut(s) 143, 224, 1587, 1654, 1678
BmiI GGNNCC 6 cut(s) 28, 106, 145, 1219, 1679, 1934
BmrFI CCNGG 2 cut(s) 1342, 2019
BmrI ACTGGG 1 cut(s) 2108
BmsI GCATC 4 cut(s) 679, 1473, 1822, 2693
BmuI ACTGGG 1 cut(s) 2108
BpiI GAAGAC 3 cut(s) 28, 1782, 2504
BpmI CTGGAG 2 cut(s) 678, 1370
Bpu14I TTCGAA 1 cut(s) 2025
BpuEI CTTGAG 2 cut(s) 77, 519
Bsa29I ATCGAT 1 cut(s) 770
BsaBI GATNNNNATC 2 cut(s) 1356, 1836
BsaHI GRCGYC 2 cut(s) 27, 105
BsaJI CCNNGG 2 cut(s) 204, 1059
BsaXI ACNNNNNCTCC 2 cut(s) 1368, 1398
Bsc4I CCNNNNNNNGG 8 cut(s) 23, 275, 298, 563, 815, 1060, 2018, 2217
Bse118I RCCGGY 2 cut(s) 140, 2883
Bse3DI GCAATG 2 cut(s) 420, 2703
Bse8I GATNNNNATC 2 cut(s) 1356, 1836
BseBI CCWGG 2 cut(s) 1342, 2019
BseCI ATCGAT 1 cut(s) 770
BseDI CCNNGG 2 cut(s) 204, 1059
BseGI GGATG 7 cut(s) 307, 412, 436, 1488, 1957, 1972, 2782
BseJI GATNNNNATC 2 cut(s) 1356, 1836
BseLI CCNNNNNNNGG 8 cut(s) 23, 275, 298, 563, 815, 1060, 2018, 2217
BseMI GCAATG 2 cut(s) 420, 2703
BseMII CTCAG 5 cut(s) 211, 1101, 2361, 2723, 2864
BseRI GAGGAG 2 cut(s) 812, 1983
BseSI GKGCMC 3 cut(s) 674, 1021, 1220
BsgI GTGCAG 1 cut(s) 888
Bsh1236I CGCG 6 cut(s) 46, 77, 89, 248, 2719, 2914
BshFI GGCC 3 cut(s) 144, 1680, 2883
BshNI GGYRCC 3 cut(s) 26, 104, 1217
BshVI ATCGAT 1 cut(s) 770
BsiHKAI GWGCWC 3 cut(s) 258, 674, 1429
BsiSI CCGG 4 cut(s) 62, 141, 2005, 2884
BslFI GGGAC 3 cut(s) 41, 1037, 1589
BslI CCNNNNNNNGG 8 cut(s) 23, 275, 298, 563, 815, 1060, 2018, 2217
BsmFI GGGAC 3 cut(s) 41, 1037, 1589
BsnI GGCC 3 cut(s) 144, 1680, 2883
Bsp119I TTCGAA 1 cut(s) 2025
Bsp1286I GDGCHC 5 cut(s) 258, 674, 1021, 1220, 1429
BspANI GGCC 3 cut(s) 144, 1680, 2883
BspCNI CTCAG 5 cut(s) 210, 1102, 2362, 2724, 2863
BspDI ATCGAT 1 cut(s) 770
BspFNI CGCG 6 cut(s) 46, 77, 89, 248, 2719, 2914
BspLI GGNNCC 6 cut(s) 28, 106, 145, 1219, 1679, 1934
BspPI GGATC 4 cut(s) 201, 1927, 1940, 2783
BspQI GCTCTTC 1 cut(s) 775
BspT104I TTCGAA 1 cut(s) 2025
BspT107I GGYRCC 3 cut(s) 26, 104, 1217
BsrDI GCAATG 2 cut(s) 420, 2703
BsrFI RCCGGY 2 cut(s) 140, 2883
BssAI RCCGGY 2 cut(s) 140, 2883
BssECI CCNNGG 2 cut(s) 204, 1059
BssNI GRCGYC 2 cut(s) 27, 105
BssSI CACGAG 1 cut(s) 179
BssT1I CCWWGG 2 cut(s) 204, 1059
Bst2BI CACGAG 1 cut(s) 179
Bst2UI CCWGG 2 cut(s) 1342, 2019
Bst4CI ACNGT 3 cut(s) 645, 2073, 2903
Bst6I CTCTTC 3 cut(s) 775, 2001, 2052
BstACI GRCGYC 2 cut(s) 27, 105
BstBI TTCGAA 1 cut(s) 2025
BstC8I GCNNGC 3 cut(s) 900, 998, 2885
BstDEI CTNAG 6 cut(s) 197, 1110, 2370, 2472, 2732, 2850
BstEII GGTNACC 2 cut(s) 1078, 2134
BstENI CCTNNNNNAGG 1 cut(s) 273
BstF5I GGATG 7 cut(s) 307, 412, 436, 1488, 1957, 1972, 2782
BstFNI CGCG 6 cut(s) 46, 77, 89, 248, 2719, 2914
BstH2I RGCGCY 2 cut(s) 30, 108
BstHHI GCGC 5 cut(s) 29, 79, 91, 107, 248
BstMWI GCNNNNNNNGC 5 cut(s) 10, 58, 262, 285, 782
BstNI CCWGG 2 cut(s) 1342, 2019
BstNSI RCATGY 1 cut(s) 1000
BstPI GGTNACC 2 cut(s) 1078, 2134
BstSCI CCNGG 2 cut(s) 1340, 2017
BstSLI GKGCMC 3 cut(s) 674, 1021, 1220
BstUI CGCG 6 cut(s) 46, 77, 89, 248, 2719, 2914
BstV2I GAAGAC 3 cut(s) 28, 1782, 2504
BstX2I RGATCY 3 cut(s) 193, 1889, 1932
BstXI CCANNNNNNTGG 1 cut(s) 1894
BstYI RGATCY 3 cut(s) 193, 1889, 1932
Bsu15I ATCGAT 1 cut(s) 770
BsuI GTATCC 2 cut(s) 1949, 2599
BsuRI GGCC 3 cut(s) 144, 1680, 2883
BsuTUI ATCGAT 1 cut(s) 770
BtgZI GCGATG 3 cut(s) 148, 506, 2236
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 7 cut(s) 307, 412, 436, 1488, 1957, 1972, 2782
BtsIMutI CAGTG 5 cut(s) 1427, 1927, 2096, 2167, 2749
Cac8I GCNNGC 3 cut(s) 900, 998, 2885
CfoI GCGC 5 cut(s) 29, 79, 91, 107, 248
Cfr10I RCCGGY 2 cut(s) 140, 2883
Cfr13I GGNCC 5 cut(s) 143, 224, 1587, 1654, 1678
ClaI ATCGAT 1 cut(s) 770
CseI GACGC 5 cut(s) 83, 95, 1172, 2708, 2920
Csp6I GTAC 6 cut(s) 314, 709, 1132, 1295, 1437, 2313
CspCI CAANNNNNGTGG 4 cut(s) 868, 903, 1327, 1362
CviAII CATG 7 cut(s) 40, 997, 1325, 1664, 1874, 1941, 2654
CviQI GTAC 6 cut(s) 314, 709, 1132, 1295, 1437, 2313
DdeI CTNAG 6 cut(s) 197, 1110, 2370, 2472, 2732, 2850
DinI GGCGCC 2 cut(s) 28, 106
DraI TTTAAA 1 cut(s) 1738
DrdI GACNNNNNNGTC 1 cut(s) 158
DriI GACNNNNNGTC 1 cut(s) 1631
DseDI GACNNNNNNGTC 1 cut(s) 158
Eam1104I CTCTTC 3 cut(s) 775, 2001, 2052
Eam1105I GACNNNNNGTC 1 cut(s) 1631
EarI CTCTTC 3 cut(s) 775, 2001, 2052
Eco130I CCWWGG 2 cut(s) 204, 1059
Eco32I GATATC 1 cut(s) 2352
Eco47I GGWCC 3 cut(s) 224, 1587, 1654
Eco57I CTGAAG 4 cut(s) 1137, 1794, 2310, 2505
Eco91I GGTNACC 2 cut(s) 1078, 2134
EcoNI CCTNNNNNAGG 1 cut(s) 273
EcoO65I GGTNACC 2 cut(s) 1078, 2134
EcoRI GAATTC 1 cut(s) 588
EcoRII CCWGG 2 cut(s) 1340, 2017
EcoRV GATATC 1 cut(s) 2352
EcoT14I CCWWGG 2 cut(s) 204, 1059
EgeI GGCGCC 2 cut(s) 28, 106
EheI GGCGCC 2 cut(s) 28, 106
ErhI CCWWGG 2 cut(s) 204, 1059
FaeI CATG 7 cut(s) 43, 1000, 1328, 1667, 1877, 1944, 2657
FalI AAGNNNNNCTT 6 cut(s) 839, 871, 1731, 1763, 1841, 1873
FaqI GGGAC 3 cut(s) 41, 1037, 1589
FatI CATG 7 cut(s) 39, 996, 1324, 1663, 1873, 1940, 2653
FauI CCCGC 3 cut(s) 66, 143, 2759
FauNDI CATATG 1 cut(s) 1915
FbaI TGATCA 3 cut(s) 763, 1492, 2821
FokI GGATG 7 cut(s) 314, 419, 443, 1495, 1964, 1979, 2789
FspBI CTAG 1 cut(s) 777
GlaI GCGC 5 cut(s) 28, 78, 90, 106, 247
GsuI CTGGAG 2 cut(s) 678, 1370
HaeII RGCGCY 2 cut(s) 30, 108
HaeIII GGCC 3 cut(s) 144, 1680, 2883
HapII CCGG 4 cut(s) 62, 141, 2005, 2884
HgaI GACGC 5 cut(s) 83, 95, 1172, 2708, 2920
HhaI GCGC 5 cut(s) 29, 79, 91, 107, 248
Hin1I GRCGYC 2 cut(s) 27, 105
Hin1II CATG 7 cut(s) 43, 1000, 1328, 1667, 1877, 1944, 2657
Hin6I GCGC 5 cut(s) 27, 77, 89, 105, 246
HinP1I GCGC 5 cut(s) 27, 77, 89, 105, 246
HincII GTYRAC 1 cut(s) 1248
HindII GTYRAC 1 cut(s) 1248
HindIII AAGCTT 2 cut(s) 978, 1170
HpaII CCGG 4 cut(s) 62, 141, 2005, 2884
HphI GGTGA 7 cut(s) 665, 713, 830, 1090, 1799, 2397, 2594
Hpy166II GTNNAC 8 cut(s) 641, 656, 672, 1248, 1897, 2157, 2313, 2738
Hpy8I GTNNAC 8 cut(s) 641, 656, 672, 1248, 1897, 2157, 2313, 2738
Hpy99I CGWCG 5 cut(s) 77, 89, 116, 152, 1188
HpyAV CCTTC 8 cut(s) 692, 1281, 1282, 1497, 1734, 1843, 2125, 2470
HpyCH4III ACNGT 3 cut(s) 645, 2073, 2903
HpyCH4IV ACGT 4 cut(s) 33, 111, 159, 368
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 58, 262, 285, 782
HpyF3I CTNAG 6 cut(s) 197, 1110, 2370, 2472, 2732, 2850
HpySE526I ACGT 4 cut(s) 33, 111, 159, 368
Hsp92I GRCGYC 2 cut(s) 27, 105
Hsp92II CATG 7 cut(s) 43, 1000, 1328, 1667, 1877, 1944, 2657
HspAI GCGC 5 cut(s) 27, 77, 89, 105, 246
KasI GGCGCC 2 cut(s) 26, 104
KroI GCCGGC 1 cut(s) 2883
KroNI GCCGGC 1 cut(s) 2885
Ksp22I TGATCA 3 cut(s) 763, 1492, 2821
LguI GCTCTTC 1 cut(s) 775
LmnI GCTCC 6 cut(s) 9, 697, 1424, 2102, 2397, 2635
LweI GCATC 4 cut(s) 679, 1473, 1822, 2693
MaeI CTAG 1 cut(s) 777
MaeII ACGT 4 cut(s) 33, 111, 159, 368
MfeI CAATTG 1 cut(s) 1827
MflI RGATCY 3 cut(s) 193, 1889, 1932
MhlI GDGCHC 5 cut(s) 258, 674, 1021, 1220, 1429
MluCI AATT 9 cut(s) 493, 588, 931, 1278, 1461, 1827, 2083, 2108, 2825
Mly113I GGCGCC 2 cut(s) 27, 105
MlyI GAGTC 4 cut(s) 809, 1121, 1892, 2002
MmeI TCCRAC 2 cut(s) 1243, 2613
MroNI GCCGGC 1 cut(s) 2883
MroXI GAANNNNTTC 3 cut(s) 1270, 1748, 2031
MseI TTAA 6 cut(s) 21, 348, 362, 1194, 1737, 2756
MslI CAYNNNNRTG 3 cut(s) 724, 1325, 2371
MspA1I CMGCKG 1 cut(s) 2763
MspI CCGG 4 cut(s) 62, 141, 2005, 2884
MspR9I CCNGG 2 cut(s) 1342, 2019
MunI CAATTG 1 cut(s) 1827
MvaI CCWGG 2 cut(s) 1342, 2019
MvnI CGCG 6 cut(s) 46, 77, 89, 248, 2719, 2914
MwoI GCNNNNNNNGC 5 cut(s) 10, 58, 262, 285, 782
NaeI GCCGGC 1 cut(s) 2885
NarI GGCGCC 2 cut(s) 27, 105
NdeI CATATG 1 cut(s) 1915
NgoMIV GCCGGC 1 cut(s) 2883
NlaIII CATG 7 cut(s) 43, 1000, 1328, 1667, 1877, 1944, 2657
NlaIV GGNNCC 6 cut(s) 28, 106, 145, 1219, 1679, 1934
NmuCI GTSAC 5 cut(s) 701, 1078, 1925, 2213, 2705
NspI RCATGY 1 cut(s) 1000
NspV TTCGAA 1 cut(s) 2025
OliI CACNNNNGTG 1 cut(s) 724
PaeI GCATGC 1 cut(s) 1000
PciSI GCTCTTC 1 cut(s) 775
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 108, 156, 171
PdiI GCCGGC 1 cut(s) 2885
PdmI GAANNNNTTC 3 cut(s) 1270, 1748, 2031
PleI GAGTC 4 cut(s) 808, 1120, 1892, 2002
PluTI GGCGCC 2 cut(s) 30, 108
PpsI GAGTC 4 cut(s) 808, 1120, 1892, 2002
Psp6I CCWGG 2 cut(s) 1340, 2017
PspEI GGTNACC 2 cut(s) 1078, 2134
PspGI CCWGG 2 cut(s) 1340, 2017
PspN4I GGNNCC 6 cut(s) 28, 106, 145, 1219, 1679, 1934
PspPI GGNCC 5 cut(s) 143, 224, 1587, 1654, 1678
PsuI RGATCY 3 cut(s) 193, 1889, 1932
RsaI GTAC 6 cut(s) 315, 710, 1133, 1296, 1438, 2314
RsaNI GTAC 6 cut(s) 314, 709, 1132, 1295, 1437, 2313
RseI CAYNNNNRTG 3 cut(s) 724, 1325, 2371
SapI GCTCTTC 1 cut(s) 775
SaqAI TTAA 6 cut(s) 21, 348, 362, 1194, 1737, 2756
Sau96I GGNCC 5 cut(s) 143, 224, 1587, 1654, 1678
SchI GAGTC 4 cut(s) 809, 1121, 1892, 2002
ScrFI CCNGG 2 cut(s) 1342, 2019
SduI GDGCHC 5 cut(s) 258, 674, 1021, 1220, 1429
SfaNI GCATC 4 cut(s) 679, 1473, 1822, 2693
SfoI GGCGCC 2 cut(s) 28, 106
SfuI TTCGAA 1 cut(s) 2025
SinI GGWCC 3 cut(s) 224, 1587, 1654
SmiMI CAYNNNNRTG 3 cut(s) 724, 1325, 2371
SmlI CTYRAG 2 cut(s) 92, 534
SmoI CTYRAG 2 cut(s) 92, 534
SphI GCATGC 1 cut(s) 1000
Sse9I AATT 9 cut(s) 493, 588, 931, 1278, 1461, 1827, 2083, 2108, 2825
SspDI GGCGCC 2 cut(s) 26, 104
SspI AATATT 2 cut(s) 1198, 2267
SspMI CTAG 1 cut(s) 777
StyD4I CCNGG 2 cut(s) 1340, 2017
StyI CCWWGG 2 cut(s) 204, 1059
TaaI ACNGT 3 cut(s) 645, 2073, 2903
TaiI ACGT 4 cut(s) 36, 114, 162, 371
TaqI TCGA 9 cut(s) 72, 630, 770, 1186, 1614, 2025, 2203, 2259, 2744
TaqII GACCGA 2 cut(s) 212, 2858
TasI AATT 9 cut(s) 493, 588, 931, 1278, 1461, 1827, 2083, 2108, 2825
TatI WGTACW 4 cut(s) 313, 708, 1131, 2312
TauI GCSGC 2 cut(s) 16, 2766
Tru1I TTAA 6 cut(s) 21, 348, 362, 1194, 1737, 2756
Tru9I TTAA 6 cut(s) 21, 348, 362, 1194, 1737, 2756
TscAI CASTG 5 cut(s) 1434, 1934, 2103, 2167, 2756
TseFI GTSAC 5 cut(s) 701, 1078, 1925, 2213, 2705
Tsp45I GTSAC 5 cut(s) 701, 1078, 1925, 2213, 2705
TspDTI ATGAA 6 cut(s) 28, 354, 615, 1313, 1463, 2655
TspGWI ACGGA 6 cut(s) 546, 568, 619, 1579, 2001, 2781
TspRI CASTG 5 cut(s) 1434, 1934, 2103, 2167, 2756
VneI GTGCAC 1 cut(s) 670
VpaK11BI GGWCC 3 cut(s) 224, 1587, 1654
XagI CCTNNNNNAGG 1 cut(s) 273
XapI RAATTY 5 cut(s) 588, 931, 1461, 2083, 2108
XceI RCATGY 1 cut(s) 1000
XmnI GAANNNNTTC 3 cut(s) 1270, 1748, 2031
XspI CTAG 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.