Rw6G032870

DNA topoisomerase 2-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003647
Physical Location & Seq
Reverse (-)
57108812 .. 57115793
6982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032870.1

Sequence Viewer

Length: 2913 bp
ATGTCGCGGAAGCTGGTCCCGCCGGAGATTTTCGACGCTCTTCACGACGCTCTCAAGCTCAACGGCGCCAATGTCGTCCTCTGCTCCGACCCCTCGCGCACCGGCCCCGACGACTACCACGTCATCGCTTCCTCCGATCACGAAAAGTTTGAGGATCTCAGAGCCAAGGGTTGCCAACTGCTAGGTCCTCAATGCGTGTTTTCTTGTGCGAACGAGCATAGGTCGCTGCCTAAACAAAAGTTTACTTGTTGCCTGGCATTGGATGGTGTCAATGTACTTGCCTCTGGTTTTGAGGCAGATGAAAAGGTTAAGATAAAGAACTTAGTAACATCAATGGGGGGAGTTTTACATACTAAAACATCTTTGGATGTTAGCTTTGTCATTGCGAAGAATGTCTTGGCTGCAAAATACAAGTGGGCTGTAAATACATTGAAGAAGCCAGTTCTTACTATTAATTGGTTATATCAATGCTGCAATGAACATCGCATTGTTCCTCAAGAATCCTTTAGGGTTCTTCCTGTTTCTGGTTTGACGATCTGTGTTACCAGAATTCCATCAGATGAACGGAAGAAGATTGAAAATCTTATCACAGAAAATGGTGGACATTATTCTGCTGAACTTACAAGGAAGTGCACACATTTGATTTGTGATATATCCTTTTATGAAGGTGATAAATACAAGGTTGCACGAATGTGGGGTCATATTTATATTGTAACTCGGAAATGGTTTGATCAATCGATTGCTAGAAGAGCTTGTCTCAGTGAAGAGTCCTTTCCAGTTCAAGGTGGTTCTCTATCTTCAAAGAAGATTGTAAAGGGCTGCTTTGCTTCACAAAATAGCCAAAGCAACTGTAGTGGGAAGTTGCAAGCTGTACCACCATCAGTGGTTGCAGATTCAAATTTTACAGCTGCTACTTGTGGTGGGATTGTGGATTCTGATCTAGAAGCTACTGTCTCACAAACCATGCTTCCAACATTTTCTTGTGCCCCTATATCTTGTAAAGAGGGGGATATCAAAGTCCCTCCCTTGGAATCAAAATGTGAGGTGACCCTTGAAGATTGTGTTGCTGATGATTCTCAGTCCGAAGACAATGACCTGTACTTGTCAGAATGTAAAATATCACTTGTTGGCTTCGAAGCTTCTGGAATGCGTAGATTGGTTAATATTATTCGCAGAGGTGGGGGCTCCCGTTATATGTCATTCAATGACAGGTTGACACACATAGTAGTTGGAACTCCTTCTGAAATTGAAAAGAAGGAGGTAAGGGGATTTGCAGCTTTAGGTGTCATTCATGTGGTTAGAGCCACCTGGCTTGAAGATTGTGATCGTGAGAAGAAAGAGATACCAGTTCTCCAGAGGCACAGTGCCTGTGATATACTTCTCCCTAAAGGAGCACTGACAGGTACGAGTGGTATGATTCAAGGGAAAATTTCTACTATTCCTCAGAGGATGCCTTCTGATCAGTTATGTGGTAATAGGAGTATGGCAACTGGAATAGATTCATTGGAGAAAAATAGAGAGGAGGTATCAGAAATAAATTGGAACGATGACATGTCTGTGAAAGAAGCAGTTGGATCATTCGATAAGAATAAACTTCCTGTCATAAAGAATAAAAGTAAGATCCAGTTACATGACAAAACAAGGGGTCAACAGAAGATACAACATGAATCAGGTGTTCAAAATGGCAAACCATCTTCTGTATTTAAGGGGAAATTTTTCCGCTTTTCAAATTCCTTTCCTGAAGACAGGAGAGGTGAAATTATTGAATGGGTAAATCAAGGGGGAGGATTGGTAATTGATGCTCATTTTAAACAGAAGGCTCACTTCACTATTGAGTGTCATGGTGTGATACCCAGATCTGTGGACACTACTCGAACTACATATGTATCAAGTCACTGGATCCGGTCATGTTTGGAGGATGGATGCTTGCTGGATGTTAGTAATCACATTCTCTATACTCCTCTTCCCTGCCGCACTCCGTTGCCTGGATTTGAAAGCTTTCGGTTTTCTGTTTCACAATACGAAGAGAAAGATAGACTGTTACTAAGAAATCTGTGTTTTACACTGGGAGCTAAATTTGTGGAGAAACTGAATAAGAAGGTTACCCATTTGTTATGCAAGTTTACCAGTGGAAGTAAGTATGAGGCTGCTTGTAAATGGGGAATACACCGAATTACCTGTGACTGGTTATATGAGTGCGTGAAGCAGAATAAAGTCATTGCTCTTGAACAATTTTATCCTAAAGAAGTTACTGCTGAAGATCGAGAGGCAGGGTTATGCACTACAAGTCAGTATCCTACACAAGATTTTCGAATGATATCTGTAGGCAATCCATCTCAATGTCCAAGTCAATCACAAGATCGGAGCTTCTCACCACCTCAAGCCATTGGTCGTAGAAATGACAATATCAGGGAAGAAGCCAAAGCATCAAGTTGCAATAAAAAGGCAAGGGTTTTGGAAGATGTTGACCAAAGCGGTCTTCTTTCTTCAGGGATAAGTTCAAGTATTCCTTCCTATACAACTGGAGACAAGAAAGTGAACACCTTTGGGGAAGTATCCCAAGTTGTTCCTGATGTAGCTTCTGCTATTGAGGACTTGTTGGAGCAGACAACTAAGATTCATGATCAGAATTCACCAGAGAGTATTTTTGCATCGGATTGTGAATTGCTTGGTCAAGACCTGCCGGGTTCTCACTCCGTGGGTGGACTATCGAGACACTGGTTAAAGAGTGCTGGGAAAAAAGATGACATCAACAATCCTCCTGGAAAACAAACTGCTGGCTTGTATGATCAGTTTAGTGAAACACAGACCGATTCTCAGGTTGTTGGATATGAGGAAGATTTGTCAGGCCGGCAAATGCTTATAGACAGAGTTCGAACGCGAAGTAGTAGTAGGACTGCTTCAAGTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000785 GO:0001932 GO:0001934 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009893 GO:0009987 GO:0010556 GO:0010562 GO:0010604 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022402 GO:0022607 GO:0030174 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031570 GO:0032268 GO:0032270 GO:0033036 GO:0033135 GO:0033138 GO:0033260 GO:0033365 GO:0033554 GO:0033674 GO:0034502 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0035561 GO:0035563 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044786 GO:0045786 GO:0045859 GO:0045860 GO:0045937 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051098 GO:0051099 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051641 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070727 GO:0071163 GO:0071165 GO:0071168 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 PTCB-BRCT

Protein Analysis

970

Amino Acids

107.5

Weight (kDa)

7.62

Isoelectric Point (pI)

51.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTCB-BRCT PF12738 92 - 153 3.3e-06 twin BRCT domain
BRCT PF00533 173 - 247 2.4e-11 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 177 - 242 1.6e-20 twin BRCT domain
BRCT PF00533 664 - 734 8.9e-08 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 669 - 729 3.7e-18 twin BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 2688
AccB1I GGYRCC 1 cut(s) 65
AccII CGCG 3 cut(s) 7, 97, 2881
AciI CCGC 5 cut(s) 7, 20, 1720, 1972, 2475
AclWI GGATC 5 cut(s) 162, 1582, 1615, 1894, 1907
AcsI RAATTY 7 cut(s) 549, 898, 1428, 1712, 1729, 2075, 2629
AcuI CTGAAG 3 cut(s) 1761, 2277, 2472
AcyI GRCGYC 1 cut(s) 66
AdeI CACNNNGTG 1 cut(s) 2698
AfaI GTAC 4 cut(s) 276, 873, 1100, 1405
AfiI CCNNNNNNNGG 6 cut(s) 259, 524, 782, 1027, 1985, 2184
AflIII ACRYGT 1 cut(s) 1551
AjiI CACGTC 1 cut(s) 121
AjnI CCWGG 4 cut(s) 252, 1307, 1984, 2761
AjuI GAANNNNNNNTTGG 4 cut(s) 380, 412, 2463, 2495
AleI CACNNNNGTG 1 cut(s) 691
Alw21I GWGCWC 2 cut(s) 635, 1396
Alw26I GTCTC 4 cut(s) 761, 958, 2520, 2707
Alw44I GTGCAC 1 cut(s) 631
AlwI GGATC 5 cut(s) 162, 1582, 1615, 1894, 1907
AlwNI CAGNNNCTG 1 cut(s) 1368
AoxI GGCC 2 cut(s) 103, 2848
ApaLI GTGCAC 1 cut(s) 631
ApeKI GCWGC 7 cut(s) 226, 401, 471, 819, 908, 1274, 2147
ApoI RAATTY 7 cut(s) 549, 898, 1428, 1712, 1729, 2075, 2629
ArsI GACNNNNNNTTYG 2 cut(s) 682, 714
AseI ATTAAT 1 cut(s) 453
Asp700I GAANNNNTTC 4 cut(s) 1237, 1498, 1715, 1998
AspLEI GCGC 2 cut(s) 68, 99
AspS9I GGNCC 3 cut(s) 16, 104, 185
AsuC2I CCSGG 1 cut(s) 2685
AsuHPI GGTGA 5 cut(s) 680, 1057, 1766, 2364, 2625
AsuII TTCGAA 3 cut(s) 1134, 2311, 2875
AvaII GGWCC 2 cut(s) 16, 185
BaeGI GKGCMC 2 cut(s) 635, 988
BaeI ACNNNNGTAYC 2 cut(s) 1869, 1902
BamHI GGATCC 1 cut(s) 1899
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 1187
BarI GAAGNNNNNNTAC 1 cut(s) 2884
BbsI GAAGAC 3 cut(s) 1092, 1749, 2471
Bbv12I GWGCWC 2 cut(s) 635, 1396
BbvI GCAGC 7 cut(s) 213, 388, 458, 806, 895, 1286, 2134
BccI CCATC 6 cut(s) 257, 562, 886, 1699, 1913, 2341
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 4 cut(s) 254, 1309, 1986, 2763
BciVI GTATCC 2 cut(s) 2304, 2566
BclI TGATCA 4 cut(s) 730, 1459, 2623, 2788
BcnI CCSGG 1 cut(s) 2685
BcoDI GTCTC 4 cut(s) 761, 958, 2520, 2707
BfaI CTAG 4 cut(s) 182, 744, 941, 2911
BfmI CTRYAG 2 cut(s) 850, 2322
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 2688
BfuI GTATCC 2 cut(s) 2304, 2566
BglII AGATCT 1 cut(s) 1856
BisI GCNGC 8 cut(s) 227, 402, 472, 820, 909, 1275, 1972, 2148
BlsI GCNGC 8 cut(s) 228, 403, 473, 821, 910, 1276, 1973, 2149
Bme1390I CCNGG 5 cut(s) 254, 1309, 1986, 2685, 2763
Bme18I GGWCC 2 cut(s) 16, 185
BmgBI CACGTC 1 cut(s) 121
BmgT120I GGNCC 3 cut(s) 16, 104, 185
BmiI GGNNCC 5 cut(s) 18, 67, 106, 1186, 1901
BmrFI CCNGG 5 cut(s) 254, 1309, 1986, 2685, 2763
BmrI ACTGGG 1 cut(s) 2075
BmsI GCATC 5 cut(s) 1440, 1789, 1913, 2435, 2660
BmuI ACTGGG 1 cut(s) 2075
BpiI GAAGAC 3 cut(s) 1092, 1749, 2471
BplI GAGNNNNNCTC 2 cut(s) 741, 773
BpmI CTGGAG 2 cut(s) 1337, 2544
Bpu14I TTCGAA 3 cut(s) 1134, 2311, 2875
BpuEI CTTGAG 3 cut(s) 38, 480, 2364
BpuMI CCSGG 1 cut(s) 2685
Bsa29I ATCGAT 1 cut(s) 737
BsaBI GATNNNNATC 2 cut(s) 936, 1323
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 165, 1026, 2697
BsaWI WCCGGW 1 cut(s) 1902
BsaXI ACNNNNNCTCC 4 cut(s) 1335, 1365, 1776, 1806
Bsc4I CCNNNNNNNGG 6 cut(s) 259, 524, 782, 1027, 1985, 2184
Bse118I RCCGGY 2 cut(s) 101, 2850
Bse3DI GCAATG 3 cut(s) 381, 481, 2217
Bse8I GATNNNNATC 2 cut(s) 936, 1323
BseBI CCWGG 4 cut(s) 254, 1309, 1986, 2763
BseCI ATCGAT 1 cut(s) 737
BseDI CCNNGG 3 cut(s) 165, 1026, 2697
BseGI GGATG 6 cut(s) 268, 373, 1455, 1924, 1928, 1939
BseJI GATNNNNATC 2 cut(s) 936, 1323
BseLI CCNNNNNNNGG 6 cut(s) 259, 524, 782, 1027, 1985, 2184
BseMI GCAATG 3 cut(s) 381, 481, 2217
BseMII CTCAG 5 cut(s) 172, 772, 1091, 1457, 2831
BseRI GAGGAG 2 cut(s) 1535, 1950
BseSI GKGCMC 2 cut(s) 635, 988
BseXI GCAGC 7 cut(s) 213, 388, 458, 806, 895, 1286, 2134
BseYI CCCAGC 1 cut(s) 2732
Bsh1236I CGCG 3 cut(s) 7, 97, 2881
BshFI GGCC 2 cut(s) 105, 2850
BshNI GGYRCC 1 cut(s) 65
BshVI ATCGAT 1 cut(s) 737
BsiHKAI GWGCWC 2 cut(s) 635, 1396
BsiSI CCGG 5 cut(s) 23, 102, 1903, 2684, 2851
BslFI GGGAC 2 cut(s) 2, 1004
BslI CCNNNNNNNGG 6 cut(s) 259, 524, 782, 1027, 1985, 2184
BsmAI GTCTC 4 cut(s) 761, 958, 2520, 2707
BsmFI GGGAC 2 cut(s) 2, 1004
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 2 cut(s) 105, 2850
Bsp119I TTCGAA 3 cut(s) 1134, 2311, 2875
Bsp1286I GDGCHC 4 cut(s) 635, 988, 1187, 1396
BspACI CCGC 5 cut(s) 7, 20, 1720, 1972, 2475
BspANI GGCC 2 cut(s) 105, 2850
BspCNI CTCAG 5 cut(s) 171, 771, 1090, 1456, 2830
BspDI ATCGAT 1 cut(s) 737
BspFNI CGCG 3 cut(s) 7, 97, 2881
BspHI TCATGA 1 cut(s) 2620
BspLI GGNNCC 5 cut(s) 18, 67, 106, 1186, 1901
BspMI ACCTGC 1 cut(s) 2688
BspPI GGATC 5 cut(s) 162, 1582, 1615, 1894, 1907
BspQI GCTCTTC 2 cut(s) 45, 742
BspT104I TTCGAA 3 cut(s) 1134, 2311, 2875
BspT107I GGYRCC 1 cut(s) 65
BsrDI GCAATG 3 cut(s) 381, 481, 2217
BsrFI RCCGGY 2 cut(s) 101, 2850
BssAI RCCGGY 2 cut(s) 101, 2850
BssECI CCNNGG 3 cut(s) 165, 1026, 2697
BssNI GRCGYC 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 165, 1026
Bst2UI CCWGG 4 cut(s) 254, 1309, 1986, 2763
Bst4CI ACNGT 4 cut(s) 851, 952, 1364, 2040
Bst6I CTCTTC 5 cut(s) 45, 742, 759, 1968, 2019
BstACI GRCGYC 1 cut(s) 66
BstBI TTCGAA 3 cut(s) 1134, 2311, 2875
BstC8I GCNNGC 4 cut(s) 867, 1928, 2779, 2852
BstDEI CTNAG 8 cut(s) 158, 322, 758, 1077, 1443, 2045, 2613, 2817
BstDSI CCRYGG 1 cut(s) 2697
BstEII GGTNACC 2 cut(s) 1045, 2101
BstF5I GGATG 6 cut(s) 268, 373, 1455, 1924, 1928, 1939
BstFNI CGCG 3 cut(s) 7, 97, 2881
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 99
BstMAI GTCTC 4 cut(s) 761, 958, 2520, 2707
BstMWI GCNNNNNNNGC 3 cut(s) 19, 223, 749
BstNI CCWGG 4 cut(s) 254, 1309, 1986, 2763
BstNSI RCATGY 1 cut(s) 1555
BstPI GGTNACC 2 cut(s) 1045, 2101
BstSCI CCNGG 5 cut(s) 252, 1307, 1984, 2683, 2761
BstSFI CTRYAG 2 cut(s) 850, 2322
BstSLI GKGCMC 2 cut(s) 635, 988
BstUI CGCG 3 cut(s) 7, 97, 2881
BstV1I GCAGC 7 cut(s) 213, 388, 458, 806, 895, 1286, 2134
BstV2I GAAGAC 3 cut(s) 1092, 1749, 2471
BstX2I RGATCY 4 cut(s) 154, 1620, 1856, 1899
BstXI CCANNNNNNTGG 1 cut(s) 1861
BstYI RGATCY 4 cut(s) 154, 1620, 1856, 1899
Bsu15I ATCGAT 1 cut(s) 737
BsuI GTATCC 2 cut(s) 2304, 2566
BsuRI GGCC 2 cut(s) 105, 2850
BsuTUI ATCGAT 1 cut(s) 737
BtgI CCRYGG 1 cut(s) 2697
BtgZI GCGATG 2 cut(s) 109, 467
BtrI CACGTC 1 cut(s) 121
BtsCI GGATG 6 cut(s) 268, 373, 1455, 1924, 1928, 1939
BtsIMutI CAGTG 8 cut(s) 766, 888, 1369, 1394, 1894, 2063, 2134, 2716
BveI ACCTGC 1 cut(s) 2688
Cac8I GCNNGC 4 cut(s) 867, 1928, 2779, 2852
CaiI CAGNNNCTG 1 cut(s) 1368
CciI TCATGA 1 cut(s) 2620
CfoI GCGC 2 cut(s) 68, 99
Cfr10I RCCGGY 2 cut(s) 101, 2850
Cfr13I GGNCC 3 cut(s) 16, 104, 185
ClaI ATCGAT 1 cut(s) 737
CseI GACGC 2 cut(s) 44, 56
Csp6I GTAC 4 cut(s) 275, 872, 1099, 1404
CspCI CAANNNNNGTGG 4 cut(s) 835, 870, 1294, 1329
CviAII CATG 8 cut(s) 964, 1292, 1552, 1631, 1664, 1841, 1908, 2621
CviQI GTAC 4 cut(s) 275, 872, 1099, 1404
DdeI CTNAG 8 cut(s) 158, 322, 758, 1077, 1443, 2045, 2613, 2817
DinI GGCGCC 1 cut(s) 67
DraI TTTAAA 1 cut(s) 1810
DraIII CACNNNGTG 1 cut(s) 2698
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1104I CTCTTC 5 cut(s) 45, 742, 759, 1968, 2019
EarI CTCTTC 5 cut(s) 45, 742, 759, 1968, 2019
Eco130I CCWWGG 2 cut(s) 165, 1026
Eco24I GRGCYC 1 cut(s) 1187
Eco32I GATATC 2 cut(s) 1012, 2319
Eco47I GGWCC 2 cut(s) 16, 185
Eco57I CTGAAG 3 cut(s) 1761, 2277, 2472
Eco91I GGTNACC 2 cut(s) 1045, 2101
EcoO109I RGGNCCY 1 cut(s) 185
EcoO65I GGTNACC 2 cut(s) 1045, 2101
EcoRI GAATTC 2 cut(s) 549, 2629
EcoRII CCWGG 4 cut(s) 252, 1307, 1984, 2761
EcoRV GATATC 2 cut(s) 1012, 2319
EcoT14I CCWWGG 2 cut(s) 165, 1026
EcoT38I GRGCYC 1 cut(s) 1187
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 165, 1026
FaeI CATG 8 cut(s) 967, 1295, 1555, 1634, 1667, 1844, 1911, 2624
FalI AAGNNNNNCTT 8 cut(s) 380, 412, 806, 838, 1808, 1840, 2494, 2526
FaqI GGGAC 2 cut(s) 2, 1004
FatI CATG 8 cut(s) 963, 1291, 1551, 1630, 1663, 1840, 1907, 2620
FauI CCCGC 1 cut(s) 27
FauNDI CATATG 1 cut(s) 1882
FbaI TGATCA 4 cut(s) 730, 1459, 2623, 2788
Fnu4HI GCNGC 8 cut(s) 227, 402, 472, 820, 909, 1275, 1972, 2148
FokI GGATG 6 cut(s) 275, 380, 1462, 1931, 1935, 1946
FriOI GRGCYC 1 cut(s) 1187
Fsp4HI GCNGC 8 cut(s) 227, 402, 472, 820, 909, 1275, 1972, 2148
FspBI CTAG 4 cut(s) 182, 744, 941, 2911
GlaI GCGC 2 cut(s) 67, 98
GluI GCNGC 8 cut(s) 227, 402, 472, 820, 909, 1275, 1972, 2148
GsaI CCCAGC 1 cut(s) 2736
GsuI CTGGAG 2 cut(s) 1337, 2544
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 2 cut(s) 105, 2850
HapII CCGG 5 cut(s) 23, 102, 1903, 2684, 2851
HgaI GACGC 2 cut(s) 44, 56
HhaI GCGC 2 cut(s) 68, 99
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 8 cut(s) 967, 1295, 1555, 1634, 1667, 1844, 1911, 2624
Hin6I GCGC 2 cut(s) 66, 97
HinP1I GCGC 2 cut(s) 66, 97
HincII GTYRAC 3 cut(s) 1215, 1649, 2467
HindII GTYRAC 3 cut(s) 1215, 1649, 2467
HindIII AAGCTT 2 cut(s) 1137, 1996
HpaII CCGG 5 cut(s) 23, 102, 1903, 2684, 2851
HphI GGTGA 5 cut(s) 680, 1057, 1766, 2364, 2625
Hpy99I CGWCG 3 cut(s) 38, 50, 113
HpyAV CCTTC 7 cut(s) 659, 1248, 1249, 1464, 1810, 2092, 2520
HpyCH4III ACNGT 4 cut(s) 851, 952, 1364, 2040
HpyCH4IV ACGT 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 223, 749
HpyF3I CTNAG 8 cut(s) 158, 322, 758, 1077, 1443, 2045, 2613, 2817
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 8 cut(s) 967, 1295, 1555, 1634, 1667, 1844, 1911, 2624
HspAI GCGC 2 cut(s) 66, 97
KasI GGCGCC 1 cut(s) 65
KroI GCCGGC 1 cut(s) 2850
KroNI GCCGGC 1 cut(s) 2852
Ksp22I TGATCA 4 cut(s) 730, 1459, 2623, 2788
LguI GCTCTTC 2 cut(s) 45, 742
LmnI GCTCC 6 cut(s) 89, 1190, 1391, 2069, 2364, 2602
Lsp1109I GCAGC 7 cut(s) 213, 388, 458, 806, 895, 1286, 2134
LweI GCATC 5 cut(s) 1440, 1789, 1913, 2435, 2660
MaeI CTAG 4 cut(s) 182, 744, 941, 2911
MaeII ACGT 1 cut(s) 120
MflI RGATCY 4 cut(s) 154, 1620, 1856, 1899
MhlI GDGCHC 4 cut(s) 635, 988, 1187, 1396
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 1 cut(s) 776
MmeI TCCRAC 6 cut(s) 111, 995, 1210, 1552, 2580, 2806
MroNI GCCGGC 1 cut(s) 2850
MroXI GAANNNNTTC 4 cut(s) 1237, 1498, 1715, 1998
MseI TTAA 6 cut(s) 309, 453, 1161, 1704, 1809, 2723
MslI CAYNNNNRTG 4 cut(s) 691, 1292, 1556, 2338
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 5 cut(s) 23, 102, 1903, 2684, 2851
MspR9I CCNGG 5 cut(s) 254, 1309, 1986, 2685, 2763
Mva1269I GAATGC 1 cut(s) 1152
MvaI CCWGG 4 cut(s) 254, 1309, 1986, 2763
MvnI CGCG 3 cut(s) 7, 97, 2881
MwoI GCNNNNNNNGC 3 cut(s) 19, 223, 749
NaeI GCCGGC 1 cut(s) 2852
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 2685
NdeI CATATG 1 cut(s) 1882
NgoMIV GCCGGC 1 cut(s) 2850
NlaIII CATG 8 cut(s) 967, 1295, 1555, 1634, 1667, 1844, 1911, 2624
NlaIV GGNNCC 5 cut(s) 18, 67, 106, 1186, 1901
NmuCI GTSAC 3 cut(s) 1045, 1892, 2180
NspI RCATGY 1 cut(s) 1555
NspV TTCGAA 3 cut(s) 1134, 2311, 2875
OliI CACNNNNGTG 1 cut(s) 691
PagI TCATGA 1 cut(s) 2620
PciI ACATGT 1 cut(s) 1551
PciSI GCTCTTC 2 cut(s) 45, 742
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 117, 132
PctI GAATGC 1 cut(s) 1152
PdiI GCCGGC 1 cut(s) 2852
PdmI GAANNNNTTC 4 cut(s) 1237, 1498, 1715, 1998
PfoI TCCNGGA 1 cut(s) 2761
PkrI GCNGC 8 cut(s) 228, 403, 473, 821, 910, 1276, 1973, 2149
PleI GAGTC 1 cut(s) 775
PluTI GGCGCC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 775
PpuMI RGGWCCY 1 cut(s) 185
PscI ACATGT 1 cut(s) 1551
PshBI ATTAAT 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 4 cut(s) 252, 1307, 1984, 2761
PspEI GGTNACC 2 cut(s) 1045, 2101
PspFI CCCAGC 1 cut(s) 2732
PspGI CCWGG 4 cut(s) 252, 1307, 1984, 2761
PspN4I GGNNCC 5 cut(s) 18, 67, 106, 1186, 1901
PspPI GGNCC 3 cut(s) 16, 104, 185
PspPPI RGGWCCY 1 cut(s) 185
PstNI CAGNNNCTG 1 cut(s) 1368
PsuI RGATCY 4 cut(s) 154, 1620, 1856, 1899
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 4 cut(s) 276, 873, 1100, 1405
RsaNI GTAC 4 cut(s) 275, 872, 1099, 1404
RseI CAYNNNNRTG 4 cut(s) 691, 1292, 1556, 2338
SapI GCTCTTC 2 cut(s) 45, 742
SaqAI TTAA 6 cut(s) 309, 453, 1161, 1704, 1809, 2723
SatI GCNGC 8 cut(s) 227, 402, 472, 820, 909, 1275, 1972, 2148
Sau96I GGNCC 3 cut(s) 16, 104, 185
SchI GAGTC 1 cut(s) 776
ScrFI CCNGG 5 cut(s) 254, 1309, 1986, 2685, 2763
SduI GDGCHC 4 cut(s) 635, 988, 1187, 1396
SfaNI GCATC 5 cut(s) 1440, 1789, 1913, 2435, 2660
SfcI CTRYAG 2 cut(s) 850, 2322
SfoI GGCGCC 1 cut(s) 67
SfuI TTCGAA 3 cut(s) 1134, 2311, 2875
SinI GGWCC 2 cut(s) 16, 185
SmiMI CAYNNNNRTG 4 cut(s) 691, 1292, 1556, 2338
SmlI CTYRAG 3 cut(s) 53, 495, 2379
SmoI CTYRAG 3 cut(s) 53, 495, 2379
SsiI CCGC 5 cut(s) 7, 20, 1720, 1972, 2475
SspDI GGCGCC 1 cut(s) 65
SspI AATATT 1 cut(s) 1165
SspMI CTAG 4 cut(s) 182, 744, 941, 2911
StyD4I CCNGG 5 cut(s) 252, 1307, 1984, 2683, 2761
StyI CCWWGG 2 cut(s) 165, 1026
TaaI ACNGT 4 cut(s) 851, 952, 1364, 2040
TaiI ACGT 1 cut(s) 123
TaqI TCGA 9 cut(s) 33, 737, 1134, 1581, 1873, 2263, 2311, 2711, 2875
TaqII GACCGA 1 cut(s) 2825
TatI WGTACW 2 cut(s) 274, 1098
TauI GCSGC 1 cut(s) 1974
Tru1I TTAA 6 cut(s) 309, 453, 1161, 1704, 1809, 2723
Tru9I TTAA 6 cut(s) 309, 453, 1161, 1704, 1809, 2723
TscAI CASTG 8 cut(s) 766, 888, 1369, 1401, 1901, 2070, 2134, 2723
TseFI GTSAC 3 cut(s) 1045, 1892, 2180
TseI GCWGC 7 cut(s) 226, 401, 471, 819, 908, 1274, 2147
Tsp45I GTSAC 3 cut(s) 1045, 1892, 2180
TspDTI ATGAA 8 cut(s) 315, 492, 576, 678, 1280, 1491, 1680, 2609
TspGWI ACGGA 3 cut(s) 580, 1968, 2686
TspRI CASTG 8 cut(s) 766, 888, 1369, 1401, 1901, 2070, 2134, 2723
VneI GTGCAC 1 cut(s) 631
VpaK11BI GGWCC 2 cut(s) 16, 185
VspI ATTAAT 1 cut(s) 453
XapI RAATTY 7 cut(s) 549, 898, 1428, 1712, 1729, 2075, 2629
XbaI TCTAGA 1 cut(s) 940
XceI RCATGY 1 cut(s) 1555
XmnI GAANNNNTTC 4 cut(s) 1237, 1498, 1715, 1998
XspI CTAG 4 cut(s) 182, 744, 941, 2911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.