Rh7DG451300

DNA topoisomerase 2-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0003647
Physical Location & Seq
Reverse (-)
64167605 .. 64171407
3803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG451300.1

Sequence Viewer

Length: 666 bp
ATGGCTGGACATTCGGCGCCGGTAAGGGTAATACTTTATTTTCCACCGCCACAACCCAAGGTGAGAGCAAACCTGGAGTTCATCCCCAAAGCCGTCCGGTACCGGTTTCAAGACTCAAGCTACTGCAATCGGGAAACCCCCAAATCTCTTCCTTCTCTGTCTCCATTTCAATGTAGAGGTCCTCAATGCGTGTTTTCTTGTGCGAACGAGCATAGGTCGCTGCCTAAACAAAAGTTTACTTGTTGCCTAGCATTGGATGGTGTCAATGTACTTGCCTCTGGTTTTGAGGCAGATGAAAAGGTTAAGATAAAGAACTTAGTAACATCAATGGGGGGAGTTTTACATACTAAAACATCTTTGGATGTTAGCTTTGTCATTGTGAAGAATGTCTTGGCTGCAAAATACGAGTGGGCTGTAAATACATTGAAGAAGCCAGTTCTTACTATTAATTGGTTATATCAATGCTGCAATGAACATCGCATTGTTCCTCAAGAATCCTTTAGGGTTCTTCCTGTTTATGGTTTGACGATCTGTGTTACCAGAATTCCATCAGATGAACGGAAGAAGATTGAAAATCTTATCACAGAAAATGGTGGACATTATTCTGCTGAACTTACAAGGAAGTGCACACATTTGATTTGTGATATATCCTTTTATGGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000785 GO:0001932 GO:0001934 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009893 GO:0009987 GO:0010556 GO:0010562 GO:0010604 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022402 GO:0022607 GO:0030174 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031570 GO:0032268 GO:0032270 GO:0033036 GO:0033135 GO:0033138 GO:0033260 GO:0033365 GO:0033554 GO:0033674 GO:0034502 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0035561 GO:0035563 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044786 GO:0045786 GO:0045859 GO:0045860 GO:0045937 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051098 GO:0051099 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051641 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070727 GO:0071163 GO:0071165 GO:0071168 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 PTCB-BRCT

Protein Analysis

221

Amino Acids

24.87

Weight (kDa)

9.12

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTCB-BRCT PF12738 90 - 151 6.8e-07 twin BRCT domain
BRCT PF00533 171 - 215 3.6e-09 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 175 - 215 1.1e-13 twin BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 2 cut(s) 16, 99
AciI CCGC 1 cut(s) 47
AcsI RAATTY 1 cut(s) 543
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 2 cut(s) 101, 270
AfiI CCNNNNNNNGG 2 cut(s) 253, 518
AgeI ACCGGT 1 cut(s) 102
AgsI TTSAA 4 cut(s) 110, 170, 427, 572
AjnI CCWGG 1 cut(s) 72
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 2 cut(s) 120, 369
AluI AGCT 2 cut(s) 120, 369
Alw21I GWGCWC 1 cut(s) 629
Alw26I GTCTC 1 cut(s) 165
Alw44I GTGCAC 1 cut(s) 625
ApaLI GTGCAC 1 cut(s) 625
ApeKI GCWGC 3 cut(s) 220, 395, 465
ApoI RAATTY 1 cut(s) 543
AseI ATTAAT 1 cut(s) 447
AsiGI ACCGGT 1 cut(s) 102
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 73
AvaII GGWCC 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 629
BanI GGYRCC 2 cut(s) 16, 99
Bbv12I GWGCWC 1 cut(s) 629
BbvI GCAGC 3 cut(s) 207, 382, 452
BccI CCATC 2 cut(s) 251, 556
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 1 cut(s) 248
BfoI RGCGCY 1 cut(s) 20
BisI GCNGC 3 cut(s) 221, 396, 466
BlsI GCNGC 3 cut(s) 222, 397, 467
Bme1390I CCNGG 1 cut(s) 74
Bme18I GGWCC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 179
BmiI GGNNCC 2 cut(s) 18, 101
BmrFI CCNGG 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 95
BpuEI CTTGAG 2 cut(s) 100, 474
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 2 cut(s) 96, 102
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 518
Bse118I RCCGGY 2 cut(s) 19, 102
Bse1I ACTGG 1 cut(s) 434
Bse3DI GCAATG 1 cut(s) 475
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 3 cut(s) 81, 262, 367
BseLI CCNNNNNNNGG 2 cut(s) 253, 518
BseMI GCAATG 1 cut(s) 475
BseNI ACTGG 1 cut(s) 434
BseSI GKGCMC 1 cut(s) 629
BseXI GCAGC 3 cut(s) 207, 382, 452
BshNI GGYRCC 2 cut(s) 16, 99
BshTI ACCGGT 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 629
BsiSI CCGG 3 cut(s) 20, 97, 103
BslI CCNNNNNNNGG 2 cut(s) 253, 518
BsmAI GTCTC 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 629
Bsp143I GATC 1 cut(s) 528
BspACI CCGC 1 cut(s) 47
BspLI GGNNCC 2 cut(s) 18, 101
BspT107I GGYRCC 2 cut(s) 16, 99
BsrDI GCAATG 1 cut(s) 475
BsrFI RCCGGY 2 cut(s) 19, 102
BsrI ACTGG 1 cut(s) 434
BssAI RCCGGY 2 cut(s) 19, 102
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 1 cut(s) 528
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 74
Bst6I CTCTTC 1 cut(s) 153
BstACI GRCGYC 1 cut(s) 17
BstDEI CTNAG 1 cut(s) 316
BstF5I GGATG 3 cut(s) 81, 262, 367
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 1 cut(s) 531
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 1 cut(s) 528
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNI CCWGG 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 72
BstSLI GKGCMC 1 cut(s) 629
BstV1I GCAGC 3 cut(s) 207, 382, 452
BtgZI GCGATG 1 cut(s) 461
BtsCI GGATG 3 cut(s) 81, 262, 367
CfoI GCGC 1 cut(s) 19
Cfr10I RCCGGY 2 cut(s) 19, 102
Cfr13I GGNCC 1 cut(s) 179
Csp6I GTAC 2 cut(s) 100, 269
CspAI ACCGGT 1 cut(s) 102
CviJI RGCY 7 cut(s) 5, 92, 120, 369, 395, 413, 433
CviKI_1 RGCY 7 cut(s) 5, 92, 120, 369, 395, 413, 433
CviQI GTAC 2 cut(s) 100, 269
DdeI CTNAG 1 cut(s) 316
DinI GGCGCC 1 cut(s) 18
DpnI GATC 1 cut(s) 530
DpnII GATC 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 72
EcoT14I CCWWGG 1 cut(s) 57
EgeI GGCGCC 1 cut(s) 18
EheI GGCGCC 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 57
FaiI YATR 6 cut(s) 213, 345, 457, 519, 647, 657
FalI AAGNNNNNCTT 2 cut(s) 374, 406
Fnu4HI GCNGC 3 cut(s) 221, 396, 466
FokI GGATG 3 cut(s) 68, 269, 374
Fsp4HI GCNGC 3 cut(s) 221, 396, 466
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 18
GluI GCNGC 3 cut(s) 221, 396, 466
GsuI CTGGAG 1 cut(s) 95
HaeII RGCGCY 1 cut(s) 20
HapII CCGG 3 cut(s) 20, 97, 103
HhaI GCGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 17
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 2 cut(s) 113, 494
HpaII CCGG 3 cut(s) 20, 97, 103
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 3 cut(s) 237, 596, 627
Hpy188I TCNGA 1 cut(s) 553
Hpy188III TCNNGA 3 cut(s) 110, 131, 491
Hpy8I GTNNAC 3 cut(s) 237, 596, 627
HpyAV CCTTC 1 cut(s) 162
HpyCH4V TGCA 4 cut(s) 126, 398, 468, 627
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 1 cut(s) 316
Hsp92I GRCGYC 1 cut(s) 17
HspAI GCGC 1 cut(s) 17
KasI GGCGCC 1 cut(s) 16
KpnI GGTACC 1 cut(s) 103
Kzo9I GATC 1 cut(s) 528
LpnPI CCDG 9 cut(s) 33, 59, 86, 110, 116, 264, 447, 525, 553
Lsp1109I GCAGC 3 cut(s) 207, 382, 452
MaeI CTAG 1 cut(s) 248
MaeIII GTNAC 2 cut(s) 319, 535
MalI GATC 1 cut(s) 530
MboI GATC 1 cut(s) 528
MboII GAAGA 6 cut(s) 140, 394, 439, 500, 574, 577
MhlI GDGCHC 1 cut(s) 629
MluCI AATT 2 cut(s) 448, 543
Mly113I GGCGCC 1 cut(s) 17
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 5 cut(s) 170, 192, 280, 286, 498
MseI TTAA 3 cut(s) 303, 447, 664
MslI CAYNNNNRTG 1 cut(s) 169
MspI CCGG 3 cut(s) 20, 97, 103
MspR9I CCNGG 1 cut(s) 74
MvaI CCWGG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 217
NarI GGCGCC 1 cut(s) 17
NdeII GATC 1 cut(s) 528
NlaIV GGNNCC 2 cut(s) 18, 101
PfeI GAWTC 1 cut(s) 494
PinAI ACCGGT 1 cut(s) 102
PkrI GCNGC 3 cut(s) 222, 397, 467
PleI GAGTC 1 cut(s) 107
PluTI GGCGCC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 107
PpuMI RGGWCCY 1 cut(s) 179
PshBI ATTAAT 1 cut(s) 447
Psp5II RGGWCCY 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 72
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 2 cut(s) 18, 101
PspPI GGNCC 1 cut(s) 179
PspPPI RGGWCCY 1 cut(s) 179
RsaI GTAC 2 cut(s) 101, 270
RsaNI GTAC 2 cut(s) 100, 269
RseI CAYNNNNRTG 1 cut(s) 169
SaqAI TTAA 3 cut(s) 303, 447, 664
SatI GCNGC 3 cut(s) 221, 396, 466
Sau3AI GATC 1 cut(s) 528
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 74
SduI GDGCHC 1 cut(s) 629
SetI ASST 7 cut(s) 63, 75, 122, 181, 218, 303, 371
SfoI GGCGCC 1 cut(s) 18
SinI GGWCC 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 169
SmlI CTYRAG 2 cut(s) 115, 489
SmoI CTYRAG 2 cut(s) 115, 489
Sse9I AATT 2 cut(s) 448, 543
SsiI CCGC 1 cut(s) 47
SspDI GGCGCC 1 cut(s) 16
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 1 cut(s) 72
StyI CCWWGG 1 cut(s) 57
TasI AATT 2 cut(s) 448, 543
TatI WGTACW 1 cut(s) 268
TfiI GAWTC 1 cut(s) 494
Tru1I TTAA 3 cut(s) 303, 447, 664
Tru9I TTAA 3 cut(s) 303, 447, 664
TseI GCWGC 3 cut(s) 220, 395, 465
TspDTI ATGAA 4 cut(s) 70, 309, 486, 570
TspGWI ACGGA 1 cut(s) 574
VneI GTGCAC 1 cut(s) 625
VpaK11BI GGWCC 1 cut(s) 179
VspI ATTAAT 1 cut(s) 447
XapI RAATTY 1 cut(s) 543
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.