Rorug06G0264300

DNA topoisomerase 2-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003647
Physical Location & Seq
Reverse (-)
41021257 .. 41021867
611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0264300.1

Sequence Viewer

Length: 507 bp
ATGTTATACTTTGATGGTCTGATTATTCTGTGGGTAGTGGTTCCAGTGACGTCGGATGAGCAAGGCAGAAATAGCAGTGATTGTTTTTATACTGATAAACAACCGGCAGCTGCAGCTCCTCCTCGAAGTTCCCGATGGACTCCAACCCCGGAGCAGATAATGCATCTGGAAGAGCTGTATCGATCTGGGATCAAGACACCGACGGCCCAACAAATCCGACAGGTGACTGCACGGCTTCAAAACTATGGGAGGGTTGAAGGCAAGAATGTATTCTACTGGTTTCAGAACCACCGAGCCAGGGAGAGGCAGAAGACACGCCGTGAACTCATGTCCATGTACCAGTCTGACTCTAAACAACAACAACAACAACAAACTTTGACTAACAACACAATTAGCAGTCTCAGGGATAAAGAATCTGCAGGAGGTAATAGGGGGATTAGGGCTCAACACTTGGTGAGGCCACCTCCTTTAGGTCACCTAAGGCTCAATAACTGTATACCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000785 GO:0001932 GO:0001934 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009893 GO:0009987 GO:0010556 GO:0010562 GO:0010604 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022402 GO:0022607 GO:0030174 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031570 GO:0032268 GO:0032270 GO:0033036 GO:0033135 GO:0033138 GO:0033260 GO:0033365 GO:0033554 GO:0033674 GO:0034502 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0035561 GO:0035563 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044786 GO:0045786 GO:0045859 GO:0045860 GO:0045937 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051098 GO:0051099 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051641 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070727 GO:0071163 GO:0071165 GO:0071168 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 PTCB-BRCT

Protein Analysis

168

Amino Acids

19.49

Weight (kDa)

9.94

Isoelectric Point (pI)

67.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 42 - 103 3.1e-14 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 53
AccI GTMKAC 1 cut(s) 496
AclWI GGATC 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 50
AdeI CACNNNGTG 2 cut(s) 320, 454
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 3 cut(s) 298, 303, 470
AgsI TTSAA 2 cut(s) 239, 257
AjnI CCWGG 1 cut(s) 296
AluBI AGCT 3 cut(s) 110, 116, 175
AluI AGCT 3 cut(s) 110, 116, 175
Alw26I GTCTC 1 cut(s) 404
AlwI GGATC 1 cut(s) 197
AoxI GGCC 2 cut(s) 204, 458
ApeKI GCWGC 3 cut(s) 107, 110, 113
Asp700I GAANNNNTTC 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 3 cut(s) 235, 466, 467
AxyI CCTNAGG 1 cut(s) 479
BanII GRGCYC 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 317
BbvI GCAGC 3 cut(s) 97, 119, 125
BccI CCATC 2 cut(s) 8, 129
BceAI ACGGC 3 cut(s) 219, 248, 303
BciT130I CCWGG 1 cut(s) 298
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 1 cut(s) 404
BfmI CTRYAG 2 cut(s) 111, 417
BisI GCNGC 3 cut(s) 108, 111, 114
BlsI GCNGC 3 cut(s) 109, 112, 115
Bme1390I CCNGG 2 cut(s) 149, 298
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 2 cut(s) 149, 298
BmsI GCATC 1 cut(s) 172
BpiI GAAGAC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 448, 480
BpuMI CCSGG 1 cut(s) 149
Bsa29I ATCGAT 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 147, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 303, 470
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 3 cut(s) 44, 281, 340
Bse21I CCTNAGG 1 cut(s) 479
BseBI CCWGG 1 cut(s) 298
BseCI ATCGAT 1 cut(s) 181
BseDI CCNNGG 2 cut(s) 147, 297
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 3 cut(s) 298, 303, 470
BseMII CTCAG 1 cut(s) 415
BseNI ACTGG 3 cut(s) 44, 281, 340
BseRI GAGGAG 2 cut(s) 108, 111
BseXI GCAGC 3 cut(s) 97, 119, 125
BsgI GTGCAG 1 cut(s) 213
BshFI GGCC 2 cut(s) 206, 460
BshVI ATCGAT 1 cut(s) 181
BsiSI CCGG 2 cut(s) 104, 149
BslI CCNNNNNNNGG 3 cut(s) 298, 303, 470
BsmAI GTCTC 1 cut(s) 404
BsnI GGCC 2 cut(s) 206, 460
Bsp1286I GDGCHC 1 cut(s) 445
Bsp143I GATC 2 cut(s) 182, 189
BspANI GGCC 2 cut(s) 206, 460
BspCNI CTCAG 1 cut(s) 414
BspDI ATCGAT 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 42
BspMAI CTGCAG 2 cut(s) 115, 421
BspPI GGATC 1 cut(s) 197
BspQI GCTCTTC 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 3 cut(s) 44, 281, 340
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 2 cut(s) 147, 297
BssMI GATC 2 cut(s) 182, 189
BssNAI GTATAC 1 cut(s) 497
BssNI GRCGYC 1 cut(s) 50
Bst1107I GTATAC 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 1 cut(s) 494
Bst6I CTCTTC 1 cut(s) 165
BstACI GRCGYC 1 cut(s) 50
BstAPI GCANNNNNTGC 1 cut(s) 160
BstDEI CTNAG 2 cut(s) 401, 479
BstEII GGTNACC 1 cut(s) 473
BstENI CCTNNNNNAGG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 61
BstKTI GATC 2 cut(s) 185, 192
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 2 cut(s) 182, 189
BstMWI GCNNNNNNNGC 3 cut(s) 72, 113, 160
BstNI CCWGG 1 cut(s) 298
BstPI GGTNACC 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 147, 296
BstSFI CTRYAG 2 cut(s) 111, 417
BstV1I GCAGC 3 cut(s) 97, 119, 125
BstV2I GAAGAC 1 cut(s) 317
BstZ17I GTATAC 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 181
Bsu36I CCTNAGG 1 cut(s) 479
BsuRI GGCC 2 cut(s) 206, 460
BsuTUI ATCGAT 1 cut(s) 181
BtsCI GGATG 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 2 cut(s) 51, 82
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 1 cut(s) 205
ClaI ATCGAT 1 cut(s) 181
Csp6I GTAC 1 cut(s) 337
CviAII CATG 2 cut(s) 328, 334
CviJI RGCY 9 cut(s) 110, 116, 175, 206, 235, 296, 443, 460, 484
CviKI_1 RGCY 9 cut(s) 110, 116, 175, 206, 235, 296, 443, 460, 484
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 2 cut(s) 401, 479
DpnI GATC 2 cut(s) 184, 191
DpnII GATC 2 cut(s) 182, 189
DraIII CACNNNGTG 2 cut(s) 320, 454
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 445
Eco81I CCTNAGG 1 cut(s) 479
Eco91I GGTNACC 1 cut(s) 473
EcoNI CCTNNNNNAGG 1 cut(s) 468
EcoO65I GGTNACC 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 296
EcoT22I ATGCAT 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 445
FaeI CATG 2 cut(s) 331, 337
FaiI YATR 7 cut(s) 7, 90, 246, 329, 335, 497, 502
FatI CATG 2 cut(s) 327, 333
FblI GTMKAC 1 cut(s) 496
Fnu4HI GCNGC 3 cut(s) 108, 111, 114
FokI GGATG 1 cut(s) 68
FriOI GRGCYC 1 cut(s) 445
Fsp4HI GCNGC 3 cut(s) 108, 111, 114
GluI GCNGC 3 cut(s) 108, 111, 114
HaeIII GGCC 2 cut(s) 206, 460
HapII CCGG 2 cut(s) 104, 149
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 2 cut(s) 331, 337
HinfI GANTC 3 cut(s) 139, 347, 413
HpaII CCGG 2 cut(s) 104, 149
HphI GGTGA 3 cut(s) 235, 466, 467
Hpy166II GTNNAC 2 cut(s) 323, 497
Hpy188I TCNGA 5 cut(s) 21, 55, 218, 285, 346
Hpy188III TCNNGA 3 cut(s) 132, 167, 193
Hpy8I GTNNAC 2 cut(s) 323, 497
Hpy99I CGWCG 2 cut(s) 55, 205
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 1 cut(s) 494
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 4 cut(s) 113, 163, 230, 419
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 113, 160
HpyF3I CTNAG 2 cut(s) 401, 479
HpySE526I ACGT 1 cut(s) 50
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 2 cut(s) 331, 337
Kzo9I GATC 2 cut(s) 182, 189
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 2 cut(s) 121, 151
Lsp1109I GCAGC 3 cut(s) 97, 119, 125
LweI GCATC 1 cut(s) 172
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 3 cut(s) 46, 223, 473
MalI GATC 2 cut(s) 184, 191
MboI GATC 2 cut(s) 182, 189
MboII GAAGA 2 cut(s) 182, 322
MhlI GDGCHC 1 cut(s) 445
MluCI AATT 1 cut(s) 390
MlyI GAGTC 2 cut(s) 133, 341
MmeI TCCRAC 3 cut(s) 33, 167, 241
MnlI CCTC 7 cut(s) 129, 132, 243, 297, 416, 450, 474
Mph1103I ATGCAT 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 269
MslI CAYNNNNRTG 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 2 cut(s) 104, 149
MspR9I CCNGG 2 cut(s) 149, 298
MvaI CCWGG 1 cut(s) 298
MwoI GCNNNNNNNGC 3 cut(s) 72, 113, 160
NciI CCSGG 1 cut(s) 149
NdeII GATC 2 cut(s) 182, 189
NlaIII CATG 2 cut(s) 331, 337
NlaIV GGNNCC 1 cut(s) 42
NmuCI GTSAC 3 cut(s) 46, 223, 473
NsiI ATGCAT 1 cut(s) 165
PciSI GCTCTTC 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 130
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 1 cut(s) 413
PkrI GCNGC 3 cut(s) 109, 112, 115
PleI GAGTC 2 cut(s) 133, 341
PpsI GAGTC 2 cut(s) 133, 341
Psp6I CCWGG 1 cut(s) 296
PspEI GGTNACC 1 cut(s) 473
PspGI CCWGG 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 1 cut(s) 205
PstI CTGCAG 2 cut(s) 115, 421
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 165
SatI GCNGC 3 cut(s) 108, 111, 114
Sau3AI GATC 2 cut(s) 182, 189
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 2 cut(s) 133, 341
ScrFI CCNGG 2 cut(s) 149, 298
SduI GDGCHC 1 cut(s) 445
SetI ASST 9 cut(s) 53, 112, 118, 177, 225, 427, 466, 475, 480
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 2 cut(s) 111, 417
SmiMI CAYNNNNRTG 1 cut(s) 332
Sse9I AATT 1 cut(s) 390
StyD4I CCNGG 2 cut(s) 147, 296
TaaI ACNGT 1 cut(s) 494
TaiI ACGT 1 cut(s) 53
TaqI TCGA 2 cut(s) 124, 181
TasI AATT 1 cut(s) 390
TfiI GAWTC 1 cut(s) 413
TscAI CASTG 2 cut(s) 51, 82
TseFI GTSAC 3 cut(s) 46, 223, 473
TseI GCWGC 3 cut(s) 107, 110, 113
Tsp45I GTSAC 3 cut(s) 46, 223, 473
TspRI CASTG 2 cut(s) 51, 82
XagI CCTNNNNNAGG 1 cut(s) 468
XmiI GTMKAC 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 269
ZraI GACGTC 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.