MD08G1159000.v1.1

DNA topoisomerase 2-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003647
Physical Location & Seq
Reverse (-)
17989075 .. 17994822
5748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1159000.v1.1.491

Sequence Viewer

Length: 2373 bp
ATGCATTATTTTTGCATGCATTGTTGCTTTTTCTTTCGCTTCCTTGATGTGAAAACAATTAGGTTGCATGAACACTTTGTTTTGACTGTTATTTTTCAATCTCTGTTCGGGTTAGTGGTCAGTTATTTGCGTGACAAATACAAGGTTGCACGAAGATGGGGCCACATTCAGATTGTAACTCAAACATGGTTTGATCAATCTATTTCTAAAAGAGCTTGTCTTCATGAGGAGTCCTATCCTGTTCAAGGTGGTTCTATATCTTCAAATAAAAGTGTAAGGGGTTGCTTTACTTTGCAGAATAGCCAAAGCAGTGCTAGTGGGAACTTGCAATTTGTGCCACCATCGGTGGTTGCAGATTCAAACTTGACAGCTGCCCCTTGTTCTGGAACTGTGGATTCTGATTTAGAAGCTACTGTCTCACAAAAGATGACCACTATATTTTCTTATGCTCCTTTTGTTGTGAAAAATGAGGATAGTAAGGCACCTACCTTTGATCCCTTGGAGTCCAAAAGTGAAGTGAACCTTGATGGTTGTGTTGCCGATGATTCTCAGTCTGAAGGTGATGACCTGTACTTGTCAGAATGTAGAATATCTCTTATTGGCTTTGAAGCTTCCGAATTGCGTAGACTGGTTAATATGGTTCGCAGAGGTGGGGGCTCCCGATATATGTCGTTCAATGATAAATTGACGCACATAGTAGTTGGTACTCCTTCAGAAATCGAAAAGAAAGAGGTAAGGGGTCTTGCAGCTTTAGGGGTCATTCATGTTGTTAGAACTGCCTGGCTTGAAGATTGTGATCGTGAGAAAAAAGAGATCCCTGTTCTCCAAAGGCACATCGCCCACGATCTACTTCTTCCTGAAGGAGCACTAACAAGTATGACTAACAAGGTTAAAGGTGGAATCTTGATGGCTCATCTAAGCATGCCTGCTGATCAATTACAAAGGAACACAAGTGCTGCAACTGGAATGGGAATGTTGGAGAAGAACAGAGAAGAGAAACCAGAAATTAACATGAAAGGTGACGGATCCATGGAAGCAGCTGTTGGACTATCCAAACGAAGTAAGCTAACTGTCCTAAATGGCAAAAGTAAGGTTCAATTAAATGATAAAACAAATGGTCTACAGAAGGTGCAACATGATTTCAATGTTCAGAACGGCAAGCCATCATCTGTCTTTAAGGGGAGAATATTTTGCTTTTCAAATTCCTTTCCTGAAGATAAGAGAGGTCAAATCATTGAATGGGTAATTCAAGGGGGAGGAGAGTTAGTTGATGGCCATATAAAACAGAAAGTGCACTTCATTATTGAATGTCATGGTGTGAGACCTATATCCCTGGATGCTACTGAAAGTACATATGTATCAAGTCACTGGATCAGGTCGTGTTTGGAGGACGGATGCTTGGTGGATGTTACTAGTCACATTCTATATGCTCCACTTCCCTGCCTGATTCCTTTGCCTGGATATGAAAACTTCCGGTTTTGTGTTTCTCAATATAATGAGAAAGATAGAGTTTTACTAAGAAATTTATGTTATACACTTGGAGCTAAATTGGTGGAAAAGTTTACGAGGAAGGTTACCCATTTGCTGTGCAAGTTTACCAATGGACCAAAGTATGAGGCTGCTCTTAAATATGGTATACATACAATTACGTCCGACTGGATATATGAATGTGTGAAGCAGAATAAAGTCGTTGCTCTTGACCAATTTTGTCCAAAAGAAGTCACTGCTGAAGATCGGGAGGCAGGGCTGTGCACCACAAGTCAGTATCCTACACAAGCTTTTCAAACAATATTTGGAGGCAATTCGTCTGAGTGTCCAAGTCAGTTACAAGACCCGAGAAACTCATCTGCTGAATTTATTGGCAATAGAAATGACAGCCTCAGAGAAGAGGCCAAAGAATCAAGCTTTTCCAATAAGAAGGCAAGGGTTTCAAAAGTAGACTCTTCTGGGGTGCATTTAAGTATTCCTGTCTGTACCACTGGAGTCAGCAAGAAAGTTCCAGGTGAAGTTTCTTCAGTTGTTCCTGATGTAGCTTCTGCTATTGAGGACTTACTGGAGCAGACAAGTAAGATTCATGATCAGCAATCGCCAGGGAGAAGTTTGTGCGATGGCAATATTTTTTCACCGGATTGTTCAATTCTTCGTGAAGAGCGCTCGGATTCTCACTCCGTCATTGGATTATCGAGATCTAACTGGTTAAACAGTGCTGGGAAAAAAGATGACATGCACAATCCTTCCGGAGAACAGAAGGCTGGCTTTTACGATGGTTTTAGCGAAACACAGACCGAGTCTCAGGTTGTTGGTTACGAGGAAGATTTGTCAGGCCGGCAAATGCTTATAGATAGAGTACGAACCCGAAGCAGCCTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000785 GO:0001932 GO:0001934 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009893 GO:0009987 GO:0010556 GO:0010562 GO:0010604 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022402 GO:0022607 GO:0030174 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031570 GO:0032268 GO:0032270 GO:0033036 GO:0033135 GO:0033138 GO:0033260 GO:0033365 GO:0033554 GO:0033674 GO:0034502 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0035561 GO:0035563 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044786 GO:0045786 GO:0045859 GO:0045860 GO:0045937 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051098 GO:0051099 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051641 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070727 GO:0071163 GO:0071165 GO:0071168 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 PTCB-BRCT

Protein Analysis

791

Amino Acids

87.78

Weight (kDa)

6.56

Isoelectric Point (pI)

58.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT_2 PF16589 391 - 471 4.8e-07 BRCT domain
BRCT PF00533 489 - 558 2.3e-11 BRCA1 C Terminus (BRCT) domain
BRCT_2 PF16589 489 - 568 5.9e-07 BRCT domain
PTCB-BRCT PF12738 492 - 553 2.7e-17 twin BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 481
AccI GTMKAC 4 cut(s) 625, 1120, 1636, 1938
AccIII TCCGGA 1 cut(s) 2237
AclWI GGATC 5 cut(s) 488, 808, 1020, 1033, 1379
AcoI YGGCCR 1 cut(s) 1273
AcsI RAATTY 3 cut(s) 1201, 1522, 1853
AcuI CTGAAG 6 cut(s) 576, 696, 879, 1233, 1749, 1998
AfaI GTAC 5 cut(s) 572, 706, 1351, 1975, 2349
AfeI AGCGCT 1 cut(s) 2153
AfiI CCNNNNNNNGG 3 cut(s) 245, 383, 1457
AhlI ACTAGT 1 cut(s) 1412
AjnI CCWGG 5 cut(s) 779, 1332, 1456, 1999, 2089
AjuI GAANNNNNNNTTGG 2 cut(s) 1026, 1058
Alw21I GWGCWC 3 cut(s) 868, 1296, 1754
Alw26I GTCTC 3 cut(s) 421, 1315, 2295
Alw44I GTGCAC 2 cut(s) 1292, 1750
AlwI GGATC 5 cut(s) 488, 808, 1020, 1033, 1379
Ama87I CYCGRG 1 cut(s) 1834
Aor13HI TCCGGA 1 cut(s) 2237
Aor51HI AGCGCT 1 cut(s) 2153
AoxI GGCC 4 cut(s) 160, 1273, 1890, 2323
ApaLI GTGCAC 2 cut(s) 1292, 1750
ApeKI GCWGC 6 cut(s) 371, 746, 956, 1037, 1619, 2361
ApoI RAATTY 3 cut(s) 1201, 1522, 1853
AspLEI GCGC 1 cut(s) 2154
AspS9I GGNCC 2 cut(s) 160, 1604
AsuHPI GGTGA 4 cut(s) 572, 1031, 2015, 2115
AvaI CYCGRG 1 cut(s) 1834
AvaII GGWCC 1 cut(s) 1604
BaeGI GKGCMC 2 cut(s) 1296, 1754
BaeI ACNNNNGTAYC 2 cut(s) 1341, 1374
BalI TGGCCA 1 cut(s) 1275
BamHI GGATCC 1 cut(s) 1025
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 1 cut(s) 659
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 3 cut(s) 868, 1296, 1754
BbvI GCAGC 5 cut(s) 358, 758, 943, 1049, 1606
BccI CCATC 8 cut(s) 150, 349, 521, 901, 1171, 1265, 2102, 2258
BceAI ACGGC 1 cut(s) 1171
BciT130I CCWGG 5 cut(s) 781, 1334, 1458, 2001, 2091
BciVI GTATCC 1 cut(s) 1776
BclI TGATCA 3 cut(s) 193, 931, 2077
BcoDI GTCTC 3 cut(s) 421, 1315, 2295
BcuI ACTAGT 1 cut(s) 1412
BfaI CTAG 2 cut(s) 315, 1413
BfmI CTRYAG 1 cut(s) 1121
BfoI RGCGCY 1 cut(s) 2155
BfuI GTATCC 1 cut(s) 1776
BglII AGATCT 1 cut(s) 2186
BisI GCNGC 6 cut(s) 372, 747, 957, 1038, 1620, 2362
BlsI GCNGC 6 cut(s) 373, 748, 958, 1039, 1621, 2363
Bme1390I CCNGG 5 cut(s) 781, 1334, 1458, 2001, 2091
Bme18I GGWCC 1 cut(s) 1604
BmeT110I CYCGRG 1 cut(s) 1834
BmgT120I GGNCC 2 cut(s) 160, 1604
BmiI GGNNCC 4 cut(s) 161, 483, 658, 1027
BmrFI CCNGG 5 cut(s) 781, 1334, 1458, 2001, 2091
BmsI GCATC 2 cut(s) 1327, 1385
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 2 cut(s) 2001, 2075
BsaBI GATNNNNATC 1 cut(s) 795
BsaI GGTCTC 1 cut(s) 1315
BsaJI CCNNGG 4 cut(s) 498, 1029, 1332, 2090
BsaWI WCCGGW 3 cut(s) 1473, 2125, 2237
BsaXI ACNNNNNCTCC 2 cut(s) 1251, 1281
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 383, 1457
Bse118I RCCGGY 1 cut(s) 2325
Bse1I ACTGG 7 cut(s) 633, 967, 1373, 1661, 1984, 2058, 2198
Bse8I GATNNNNATC 1 cut(s) 795
BseAI TCCGGA 1 cut(s) 2237
BseBI CCWGG 5 cut(s) 781, 1334, 1458, 2001, 2091
BseDI CCNNGG 4 cut(s) 498, 1029, 1332, 2090
BseGI GGATG 3 cut(s) 1342, 1400, 1411
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 3 cut(s) 245, 383, 1457
BseMII CTCAG 4 cut(s) 563, 1800, 1894, 2306
BseNI ACTGG 7 cut(s) 633, 967, 1373, 1661, 1984, 2058, 2198
BseRI GAGGAG 2 cut(s) 242, 1272
BseSI GKGCMC 2 cut(s) 1296, 1754
BseXI GCAGC 5 cut(s) 358, 758, 943, 1049, 1606
BseYI CCCAGC 1 cut(s) 2207
BshFI GGCC 4 cut(s) 162, 1275, 1892, 2325
BshNI GGYRCC 1 cut(s) 481
BsiHKAI GWGCWC 3 cut(s) 868, 1296, 1754
BsiHKCI CYCGRG 1 cut(s) 1834
BsiSI CCGG 4 cut(s) 1474, 2126, 2238, 2326
BslI CCNNNNNNNGG 3 cut(s) 245, 383, 1457
BsmAI GTCTC 3 cut(s) 421, 1315, 2295
BsnI GGCC 4 cut(s) 162, 1275, 1892, 2325
Bso31I GGTCTC 1 cut(s) 1315
BsoBI CYCGRG 1 cut(s) 1834
Bsp1286I GDGCHC 4 cut(s) 659, 868, 1296, 1754
Bsp13I TCCGGA 1 cut(s) 2237
Bsp19I CCATGG 1 cut(s) 1029
BspANI GGCC 4 cut(s) 162, 1275, 1892, 2325
BspCNI CTCAG 4 cut(s) 562, 1801, 1893, 2305
BspEI TCCGGA 1 cut(s) 2237
BspHI TCATGA 2 cut(s) 223, 2074
BspLI GGNNCC 4 cut(s) 161, 483, 658, 1027
BspPI GGATC 5 cut(s) 488, 808, 1020, 1033, 1379
BspQI GCTCTTC 1 cut(s) 2142
BspT107I GGYRCC 1 cut(s) 481
BspTNI GGTCTC 1 cut(s) 1315
BsrFI RCCGGY 1 cut(s) 2325
BsrI ACTGG 7 cut(s) 633, 967, 1373, 1661, 1984, 2058, 2198
BssAI RCCGGY 1 cut(s) 2325
BssECI CCNNGG 4 cut(s) 498, 1029, 1332, 2090
BssNAI GTATAC 1 cut(s) 1637
BssT1I CCWWGG 2 cut(s) 498, 1029
Bst1107I GTATAC 1 cut(s) 1637
Bst2UI CCWGG 5 cut(s) 781, 1334, 1458, 2001, 2091
Bst4CI ACNGT 5 cut(s) 88, 391, 415, 1072, 2204
Bst6I CTCTTC 4 cut(s) 987, 1881, 1948, 2142
BstAPI GCANNNNNTGC 1 cut(s) 334
BstC8I GCNNGC 6 cut(s) 17, 923, 927, 1160, 2254, 2327
BstDEI CTNAG 6 cut(s) 549, 917, 1517, 1809, 1880, 2292
BstDSI CCRYGG 1 cut(s) 1029
BstEII GGTNACC 1 cut(s) 1573
BstENI CCTNNNNNAGG 1 cut(s) 243
BstF5I GGATG 3 cut(s) 1342, 1400, 1411
BstH2I RGCGCY 1 cut(s) 2155
BstHHI GCGC 1 cut(s) 2154
BstMAI GTCTC 3 cut(s) 421, 1315, 2295
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstNI CCWGG 5 cut(s) 781, 1334, 1458, 2001, 2091
BstNSI RCATGY 3 cut(s) 19, 925, 2227
BstPI GGTNACC 1 cut(s) 1573
BstSCI CCNGG 5 cut(s) 779, 1332, 1456, 1999, 2089
BstSFI CTRYAG 1 cut(s) 1121
BstSLI GKGCMC 2 cut(s) 1296, 1754
BstV1I GCAGC 5 cut(s) 358, 758, 943, 1049, 1606
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 3 cut(s) 813, 1025, 2186
BstYI RGATCY 3 cut(s) 813, 1025, 2186
BstZ17I GTATAC 1 cut(s) 1637
BsuI GTATCC 1 cut(s) 1776
BsuRI GGCC 4 cut(s) 162, 1275, 1892, 2325
BtgI CCRYGG 1 cut(s) 1029
BtgZI GCGATG 2 cut(s) 820, 2121
BtsCI GGATG 3 cut(s) 1342, 1400, 1411
BtsI GCAGTG 2 cut(s) 316, 1722
BtsIMutI CAGTG 5 cut(s) 316, 1366, 1722, 1977, 2209
Cac8I GCNNGC 6 cut(s) 17, 923, 927, 1160, 2254, 2327
CciI TCATGA 2 cut(s) 223, 2074
CfoI GCGC 1 cut(s) 2154
Cfr10I RCCGGY 1 cut(s) 2325
Cfr13I GGNCC 2 cut(s) 160, 1604
CseI GACGC 1 cut(s) 697
Csp6I GTAC 5 cut(s) 571, 705, 1350, 1974, 2348
CviQI GTAC 5 cut(s) 571, 705, 1350, 1974, 2348
DdeI CTNAG 6 cut(s) 549, 917, 1517, 1809, 1880, 2292
EaeI YGGCCR 1 cut(s) 1273
Eam1104I CTCTTC 4 cut(s) 987, 1881, 1948, 2142
EarI CTCTTC 4 cut(s) 987, 1881, 1948, 2142
Eco130I CCWWGG 2 cut(s) 498, 1029
Eco24I GRGCYC 1 cut(s) 659
Eco31I GGTCTC 1 cut(s) 1315
Eco47I GGWCC 1 cut(s) 1604
Eco47III AGCGCT 1 cut(s) 2153
Eco57I CTGAAG 6 cut(s) 576, 696, 879, 1233, 1749, 1998
Eco88I CYCGRG 1 cut(s) 1834
Eco91I GGTNACC 1 cut(s) 1573
EcoNI CCTNNNNNAGG 1 cut(s) 243
EcoO65I GGTNACC 1 cut(s) 1573
EcoRII CCWGG 5 cut(s) 779, 1332, 1456, 1999, 2089
EcoT14I CCWWGG 2 cut(s) 498, 1029
EcoT22I ATGCAT 2 cut(s) 6, 21
EcoT38I GRGCYC 1 cut(s) 659
ErhI CCWWGG 2 cut(s) 498, 1029
FalI AAGNNNNNCTT 4 cut(s) 507, 539, 2240, 2272
FauNDI CATATG 1 cut(s) 1354
FbaI TGATCA 3 cut(s) 193, 931, 2077
FblI GTMKAC 4 cut(s) 625, 1120, 1636, 1938
Fnu4HI GCNGC 6 cut(s) 372, 747, 957, 1038, 1620, 2362
FokI GGATG 3 cut(s) 1349, 1407, 1418
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 6 cut(s) 372, 747, 957, 1038, 1620, 2362
FspBI CTAG 2 cut(s) 315, 1413
GlaI GCGC 1 cut(s) 2153
GluI GCNGC 6 cut(s) 372, 747, 957, 1038, 1620, 2362
GsaI CCCAGC 1 cut(s) 2211
GsuI CTGGAG 2 cut(s) 2001, 2075
HaeII RGCGCY 1 cut(s) 2155
HaeIII GGCC 4 cut(s) 162, 1275, 1892, 2325
HapII CCGG 4 cut(s) 1474, 2126, 2238, 2326
HgaI GACGC 1 cut(s) 697
HhaI GCGC 1 cut(s) 2154
Hin6I GCGC 1 cut(s) 2152
HinP1I GCGC 1 cut(s) 2152
HindIII AAGCTT 3 cut(s) 609, 1776, 1903
HpaII CCGG 4 cut(s) 1474, 2126, 2238, 2326
HphI GGTGA 4 cut(s) 572, 1031, 2015, 2115
Hpy166II GTNNAC 9 cut(s) 520, 626, 1121, 1294, 1563, 1596, 1637, 1752, 1939
Hpy8I GTNNAC 9 cut(s) 520, 626, 1121, 1294, 1563, 1596, 1637, 1752, 1939
HpyAV CCTTC 8 cut(s) 551, 720, 854, 1120, 1564, 1912, 2242, 2244
HpyCH4III ACNGT 5 cut(s) 88, 391, 415, 1072, 2204
HpyCH4IV ACGT 1 cut(s) 1649
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
HpyF3I CTNAG 6 cut(s) 549, 917, 1517, 1809, 1880, 2292
HpySE526I ACGT 1 cut(s) 1649
HspAI GCGC 1 cut(s) 2152
Kpn2I TCCGGA 1 cut(s) 2237
KroI GCCGGC 1 cut(s) 2325
KroNI GCCGGC 1 cut(s) 2327
Ksp22I TGATCA 3 cut(s) 193, 931, 2077
LguI GCTCTTC 1 cut(s) 2142
LmnI GCTCC 6 cut(s) 454, 662, 863, 1435, 1541, 2056
Lsp1109I GCAGC 5 cut(s) 358, 758, 943, 1049, 1606
LweI GCATC 2 cut(s) 1327, 1385
MaeI CTAG 2 cut(s) 315, 1413
MaeII ACGT 1 cut(s) 1649
MflI RGATCY 3 cut(s) 813, 1025, 2186
MhlI GDGCHC 4 cut(s) 659, 868, 1296, 1754
MlsI TGGCCA 1 cut(s) 1275
MluNI TGGCCA 1 cut(s) 1275
MlyI GAGTC 5 cut(s) 239, 512, 1934, 1992, 2297
MmeI TCCRAC 3 cut(s) 957, 1024, 1677
Mox20I TGGCCA 1 cut(s) 1275
Mph1103I ATGCAT 2 cut(s) 6, 21
MroI TCCGGA 1 cut(s) 2237
MroNI GCCGGC 1 cut(s) 2325
MscI TGGCCA 1 cut(s) 1275
MseI TTAA 8 cut(s) 633, 891, 1008, 1100, 1176, 1626, 1958, 2198
MslI CAYNNNNRTG 1 cut(s) 154
Msp20I TGGCCA 1 cut(s) 1275
MspA1I CMGCKG 2 cut(s) 371, 1040
MspI CCGG 4 cut(s) 1474, 2126, 2238, 2326
MspR9I CCNGG 5 cut(s) 781, 1334, 1458, 2001, 2091
MvaI CCWGG 5 cut(s) 781, 1334, 1458, 2001, 2091
MwoI GCNNNNNNNGC 1 cut(s) 334
NaeI GCCGGC 1 cut(s) 2327
NcoI CCATGG 1 cut(s) 1029
NdeI CATATG 1 cut(s) 1354
NgoMIV GCCGGC 1 cut(s) 2325
NlaIV GGNNCC 4 cut(s) 161, 483, 658, 1027
NmuCI GTSAC 5 cut(s) 131, 1019, 1364, 1415, 1720
NsiI ATGCAT 2 cut(s) 6, 21
NspI RCATGY 3 cut(s) 19, 925, 2227
PaeI GCATGC 2 cut(s) 19, 925
PagI TCATGA 2 cut(s) 223, 2074
PciSI GCTCTTC 1 cut(s) 2142
PdiI GCCGGC 1 cut(s) 2327
PfeI GAWTC 8 cut(s) 356, 395, 545, 900, 1447, 1898, 2071, 2159
PflFI GACNNNGTC 1 cut(s) 2287
PkrI GCNGC 6 cut(s) 373, 748, 958, 1039, 1621, 2363
PleI GAGTC 5 cut(s) 238, 511, 1934, 1991, 2296
PpsI GAGTC 5 cut(s) 238, 511, 1934, 1991, 2296
Psp6I CCWGG 5 cut(s) 779, 1332, 1456, 1999, 2089
PspEI GGTNACC 1 cut(s) 1573
PspFI CCCAGC 1 cut(s) 2207
PspGI CCWGG 5 cut(s) 779, 1332, 1456, 1999, 2089
PspN4I GGNNCC 4 cut(s) 161, 483, 658, 1027
PspPI GGNCC 2 cut(s) 160, 1604
PsuI RGATCY 3 cut(s) 813, 1025, 2186
PsyI GACNNNGTC 1 cut(s) 2287
PvuII CAGCTG 2 cut(s) 371, 1040
RsaI GTAC 5 cut(s) 572, 706, 1351, 1975, 2349
RsaNI GTAC 5 cut(s) 571, 705, 1350, 1974, 2348
RseI CAYNNNNRTG 1 cut(s) 154
SapI GCTCTTC 1 cut(s) 2142
SaqAI TTAA 8 cut(s) 633, 891, 1008, 1100, 1176, 1626, 1958, 2198
SatI GCNGC 6 cut(s) 372, 747, 957, 1038, 1620, 2362
Sau96I GGNCC 2 cut(s) 160, 1604
SchI GAGTC 5 cut(s) 239, 512, 1934, 1992, 2297
ScrFI CCNGG 5 cut(s) 781, 1334, 1458, 2001, 2091
SduI GDGCHC 4 cut(s) 659, 868, 1296, 1754
SfaNI GCATC 2 cut(s) 1327, 1385
SfcI CTRYAG 1 cut(s) 1121
SinI GGWCC 1 cut(s) 1604
SmiMI CAYNNNNRTG 1 cut(s) 154
SpeI ACTAGT 1 cut(s) 1412
SphI GCATGC 2 cut(s) 19, 925
SspI AATATT 3 cut(s) 1188, 1791, 2116
SspMI CTAG 2 cut(s) 315, 1413
StyD4I CCNGG 5 cut(s) 779, 1332, 1456, 1999, 2089
StyI CCWWGG 2 cut(s) 498, 1029
TaaI ACNGT 5 cut(s) 88, 391, 415, 1072, 2204
TaiI ACGT 1 cut(s) 1652
TaqI TCGA 2 cut(s) 720, 2183
TaqII GACCGA 1 cut(s) 2300
TatI WGTACW 2 cut(s) 570, 1349
TfiI GAWTC 8 cut(s) 356, 395, 545, 900, 1447, 1898, 2071, 2159
Tru1I TTAA 8 cut(s) 633, 891, 1008, 1100, 1176, 1626, 1958, 2198
Tru9I TTAA 8 cut(s) 633, 891, 1008, 1100, 1176, 1626, 1958, 2198
TscAI CASTG 5 cut(s) 316, 1373, 1729, 1984, 2209
TseFI GTSAC 5 cut(s) 131, 1019, 1364, 1415, 1720
TseI GCWGC 6 cut(s) 371, 746, 956, 1037, 1619, 2361
Tsp45I GTSAC 5 cut(s) 131, 1019, 1364, 1415, 1720
TspDTI ATGAA 8 cut(s) 84, 212, 752, 1028, 1288, 1479, 1680, 2063
TspGWI ACGGA 3 cut(s) 1038, 1407, 2158
TspRI CASTG 5 cut(s) 316, 1373, 1729, 1984, 2209
Tth111I GACNNNGTC 1 cut(s) 2287
VneI GTGCAC 2 cut(s) 1292, 1750
VpaK11BI GGWCC 1 cut(s) 1604
XagI CCTNNNNNAGG 1 cut(s) 243
XapI RAATTY 3 cut(s) 1201, 1522, 1853
XceI RCATGY 3 cut(s) 19, 925, 2227
XmiI GTMKAC 4 cut(s) 625, 1120, 1636, 1938
XspI CTAG 2 cut(s) 315, 1413
Zsp2I ATGCAT 2 cut(s) 6, 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.