pycom348g00060

DNA topoisomerase 2-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000348
OG0003647
Physical Location & Seq
Forward (+)
60497 .. 63049
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom348g00060.2

Sequence Viewer

Length: 990 bp
ATGGAATTCCTCGACGGATGCTTGGTGGATGTTACTAGTCACATTCTATATGCTCCACTTCCCTGCCTGATTCCTTTGCCTGGATATGAAAACTTTCGGTTTTGTGTTTCTCAATATAATGAGAAAGATAGAGTTTTACTAAGAAATTTATGTTATACACTTGGAGCTAAATTTGTGGAAAAGTTTACGAGGAAGGTTACCCATTTGCTATGCAAGTTTACCAATGGACCAAAGTATGAGGCTGCTCTTAAATATGGTATACACACAATTACGTCCGACTGGATATATGAATGTGTGAAGCAGAATAAAGTCGTTGCTCTTGACCAATTTTGTCCAAAAGAAGTCACTGCTGAAGATCAAGAGGCAGGGCTGTGCACCACGAGTCAGTATCCTACACAAGCTGTTCAAACAATATTTGGCGGCAATTCATCTGAGTGTCCAAGTCAGTTACAAGACCTGAGAAACTCATCAGCTGAATTTATTGGCAATAGAAATGACAGCCTCAGAGAAGAGGCCAAAGAATCAAGCTTTTCCAATAAGAAGGCAAGGGTTTCAAAAGTTGACTCTTCTGGGGTGCATTTAAGTATTCCTGTCTGTACCACTGGAGTCAGCAAGAAAGTTCCGGGTGAAGTTTCTTCAGTTGTTCCTGATGTAGCTTCTGCTATTGAGGACTTACTGGAGCAGACAATCATGATCGAGCAATCGCCAGGGAGGAGTTTGTGTGATAGCAATATTTTTTCACCGGATTGTTCAACTCTTCGTGAAGAGCGCTCAGATTCTCACTCCGTCATTGGATTATCGAGATCTAACTGGTTAAACAGTGCTGGGAAAAAAGATGACATCCACAATCCTTCCGGAGAACAAAAGGCTGGCTTTTACGATGGTTTTAGCGAAACACAGACCGAGTCTCAGGTTGTTGGATACGAGGAAGATTTGTCAGGCCGGCAAATGCTTATAGACAGAGTACGAACCCGAAGCAGCCTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000785 GO:0001932 GO:0001934 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009893 GO:0009987 GO:0010556 GO:0010562 GO:0010604 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022402 GO:0022607 GO:0030174 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031570 GO:0032268 GO:0032270 GO:0033036 GO:0033135 GO:0033138 GO:0033260 GO:0033365 GO:0033554 GO:0033674 GO:0034502 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0035561 GO:0035563 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044786 GO:0045786 GO:0045859 GO:0045860 GO:0045937 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051098 GO:0051099 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051641 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070727 GO:0071163 GO:0071165 GO:0071168 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 PTCB-BRCT

Protein Analysis

330

Amino Acids

36.49

Weight (kDa)

5.31

Isoelectric Point (pI)

56.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 259
AccIII TCCGGA 1 cut(s) 854
AciI CCGC 1 cut(s) 420
AcsI RAATTY 4 cut(s) 5, 145, 170, 476
AcuI CTGAAG 2 cut(s) 372, 621
AfaI GTAC 2 cut(s) 598, 966
AfeI AGCGCT 1 cut(s) 770
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 3 cut(s) 407, 555, 753
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 2 cut(s) 79, 706
AluBI AGCT 5 cut(s) 167, 401, 473, 528, 656
AluI AGCT 5 cut(s) 167, 401, 473, 528, 656
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 1 cut(s) 912
Alw44I GTGCAC 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 854
Aor51HI AGCGCT 1 cut(s) 770
AoxI GGCC 2 cut(s) 513, 940
ApaLI GTGCAC 1 cut(s) 373
ApeKI GCWGC 2 cut(s) 242, 978
ApoI RAATTY 4 cut(s) 5, 145, 170, 476
Asp700I GAANNNNTTC 1 cut(s) 93
AspLEI GCGC 1 cut(s) 771
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 1 cut(s) 624
AsuHPI GGTGA 2 cut(s) 638, 732
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 377
BauI CACGAG 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 1 cut(s) 229
BccI CCATC 1 cut(s) 875
BciT130I CCWGG 2 cut(s) 81, 708
BciVI GTATCC 2 cut(s) 399, 914
BcnI CCSGG 1 cut(s) 624
BcoDI GTCTC 1 cut(s) 912
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 1 cut(s) 36
BfoI RGCGCY 1 cut(s) 772
BfuI GTATCC 2 cut(s) 399, 914
BglII AGATCT 1 cut(s) 803
BisI GCNGC 3 cut(s) 243, 421, 979
BlsI GCNGC 3 cut(s) 244, 422, 980
Bme1390I CCNGG 3 cut(s) 81, 624, 708
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 227
BmrFI CCNGG 3 cut(s) 81, 624, 708
BmsI GCATC 1 cut(s) 8
BpmI CTGGAG 2 cut(s) 624, 698
BpuMI CCSGG 1 cut(s) 624
BsaJI CCNNGG 1 cut(s) 707
BsaWI WCCGGW 2 cut(s) 742, 854
BsaXI ACNNNNNCTCC 2 cut(s) 706, 736
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse118I RCCGGY 1 cut(s) 942
Bse1I ACTGG 4 cut(s) 284, 607, 681, 815
BseAI TCCGGA 1 cut(s) 854
BseBI CCWGG 2 cut(s) 81, 708
BseDI CCNNGG 1 cut(s) 707
BseGI GGATG 3 cut(s) 23, 34, 840
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMII CTCAG 5 cut(s) 423, 449, 517, 786, 923
BseNI ACTGG 4 cut(s) 284, 607, 681, 815
BseRI GAGGAG 1 cut(s) 727
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 1 cut(s) 229
BseYI CCCAGC 1 cut(s) 824
BshFI GGCC 2 cut(s) 515, 942
BsiHKAI GWGCWC 1 cut(s) 377
BsiSI CCGG 4 cut(s) 623, 743, 855, 943
BslI CCNNNNNNNGG 1 cut(s) 80
BsmAI GTCTC 1 cut(s) 912
BsnI GGCC 2 cut(s) 515, 942
Bsp1286I GDGCHC 1 cut(s) 377
Bsp13I TCCGGA 1 cut(s) 854
Bsp143I GATC 3 cut(s) 355, 693, 803
BspACI CCGC 1 cut(s) 420
BspANI GGCC 2 cut(s) 515, 942
BspCNI CTCAG 5 cut(s) 424, 450, 516, 785, 922
BspEI TCCGGA 1 cut(s) 854
BspHI TCATGA 1 cut(s) 690
BspQI GCTCTTC 1 cut(s) 759
BsrFI RCCGGY 1 cut(s) 942
BsrI ACTGG 4 cut(s) 284, 607, 681, 815
BssAI RCCGGY 1 cut(s) 942
BssECI CCNNGG 1 cut(s) 707
BssMI GATC 3 cut(s) 355, 693, 803
BssNAI GTATAC 1 cut(s) 260
BssSI CACGAG 1 cut(s) 379
Bst1107I GTATAC 1 cut(s) 260
Bst2BI CACGAG 1 cut(s) 379
Bst2UI CCWGG 2 cut(s) 81, 708
Bst4CI ACNGT 1 cut(s) 821
Bst6I CTCTTC 4 cut(s) 504, 571, 759, 762
BstC8I GCNNGC 2 cut(s) 871, 944
BstDEI CTNAG 6 cut(s) 140, 432, 458, 503, 772, 909
BstEII GGTNACC 1 cut(s) 196
BstF5I GGATG 3 cut(s) 23, 34, 840
BstH2I RGCGCY 1 cut(s) 772
BstHHI GCGC 1 cut(s) 771
BstKTI GATC 3 cut(s) 358, 696, 806
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 3 cut(s) 355, 693, 803
BstNI CCWGG 2 cut(s) 81, 708
BstPI GGTNACC 1 cut(s) 196
BstSCI CCNGG 3 cut(s) 79, 622, 706
BstSLI GKGCMC 1 cut(s) 377
BstV1I GCAGC 1 cut(s) 229
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
BstZ17I GTATAC 1 cut(s) 260
BsuI GTATCC 2 cut(s) 399, 914
BsuRI GGCC 2 cut(s) 515, 942
BtsCI GGATG 3 cut(s) 23, 34, 840
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 3 cut(s) 345, 600, 826
Cac8I GCNNGC 2 cut(s) 871, 944
CciI TCATGA 1 cut(s) 690
CfoI GCGC 1 cut(s) 771
Cfr10I RCCGGY 1 cut(s) 942
Cfr13I GGNCC 1 cut(s) 227
Csp6I GTAC 2 cut(s) 597, 965
CviAII CATG 1 cut(s) 691
CviQI GTAC 2 cut(s) 597, 965
DdeI CTNAG 6 cut(s) 140, 432, 458, 503, 772, 909
DpnI GATC 3 cut(s) 357, 695, 805
DpnII GATC 3 cut(s) 355, 693, 803
Eam1104I CTCTTC 4 cut(s) 504, 571, 759, 762
EarI CTCTTC 4 cut(s) 504, 571, 759, 762
Eco47I GGWCC 1 cut(s) 227
Eco47III AGCGCT 1 cut(s) 770
Eco57I CTGAAG 2 cut(s) 372, 621
Eco91I GGTNACC 1 cut(s) 196
EcoO65I GGTNACC 1 cut(s) 196
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 2 cut(s) 79, 706
FaeI CATG 1 cut(s) 694
FalI AAGNNNNNCTT 2 cut(s) 857, 889
FatI CATG 1 cut(s) 690
FblI GTMKAC 1 cut(s) 259
Fnu4HI GCNGC 3 cut(s) 243, 421, 979
FokI GGATG 3 cut(s) 30, 41, 827
Fsp4HI GCNGC 3 cut(s) 243, 421, 979
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 770
GluI GCNGC 3 cut(s) 243, 421, 979
GsaI CCCAGC 1 cut(s) 828
GsuI CTGGAG 2 cut(s) 624, 698
HaeII RGCGCY 1 cut(s) 772
HaeIII GGCC 2 cut(s) 515, 942
HapII CCGG 4 cut(s) 623, 743, 855, 943
HhaI GCGC 1 cut(s) 771
Hin1II CATG 1 cut(s) 694
Hin6I GCGC 1 cut(s) 769
HinP1I GCGC 1 cut(s) 769
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 526
HinfI GANTC 7 cut(s) 70, 382, 521, 563, 606, 776, 905
HpaII CCGG 4 cut(s) 623, 743, 855, 943
HphI GGTGA 2 cut(s) 638, 732
Hpy166II GTNNAC 5 cut(s) 186, 219, 260, 375, 562
Hpy188I TCNGA 4 cut(s) 277, 433, 506, 775
Hpy188III TCNNGA 8 cut(s) 320, 359, 647, 691, 761, 801, 855, 987
Hpy8I GTNNAC 5 cut(s) 186, 219, 260, 375, 562
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 3 cut(s) 187, 535, 861
HpyCH4III ACNGT 1 cut(s) 821
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 3 cut(s) 213, 375, 577
HpyF3I CTNAG 6 cut(s) 140, 432, 458, 503, 772, 909
HpySE526I ACGT 1 cut(s) 272
Hsp92II CATG 1 cut(s) 694
HspAI GCGC 1 cut(s) 769
Kpn2I TCCGGA 1 cut(s) 854
KroI GCCGGC 1 cut(s) 942
KroNI GCCGGC 1 cut(s) 944
Kzo9I GATC 3 cut(s) 355, 693, 803
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 3 cut(s) 58, 164, 679
Lsp1109I GCAGC 1 cut(s) 229
LweI GCATC 1 cut(s) 8
MaeI CTAG 1 cut(s) 36
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 5 cut(s) 31, 38, 196, 343, 447
MalI GATC 3 cut(s) 357, 695, 805
MboI GATC 3 cut(s) 355, 693, 803
MboII GAAGA 7 cut(s) 365, 521, 558, 627, 749, 776, 941
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 7 cut(s) 5, 145, 170, 267, 326, 424, 476
MlyI GAGTC 4 cut(s) 391, 557, 615, 914
MmeI TCCRAC 2 cut(s) 300, 898
MnlI CCTC 9 cut(s) 20, 183, 232, 355, 505, 512, 661, 705, 919
MroI TCCGGA 1 cut(s) 854
MroNI GCCGGC 1 cut(s) 942
MroXI GAANNNNTTC 1 cut(s) 93
MseI TTAA 3 cut(s) 249, 581, 815
MslI CAYNNNNRTG 1 cut(s) 433
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 4 cut(s) 623, 743, 855, 943
MspR9I CCNGG 3 cut(s) 81, 624, 708
MvaI CCWGG 2 cut(s) 81, 708
NaeI GCCGGC 1 cut(s) 944
NciI CCSGG 1 cut(s) 624
NdeII GATC 3 cut(s) 355, 693, 803
NgoMIV GCCGGC 1 cut(s) 942
NlaIII CATG 1 cut(s) 694
NmuCI GTSAC 2 cut(s) 38, 343
PagI TCATGA 1 cut(s) 690
PciSI GCTCTTC 1 cut(s) 759
PdiI GCCGGC 1 cut(s) 944
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 70, 521, 776
PflFI GACNNNGTC 1 cut(s) 904
PkrI GCNGC 3 cut(s) 244, 422, 980
PleI GAGTC 4 cut(s) 390, 557, 614, 913
PpsI GAGTC 4 cut(s) 390, 557, 614, 913
Psp6I CCWGG 2 cut(s) 79, 706
PspEI GGTNACC 1 cut(s) 196
PspFI CCCAGC 1 cut(s) 824
PspGI CCWGG 2 cut(s) 79, 706
PspPI GGNCC 1 cut(s) 227
PsuI RGATCY 1 cut(s) 803
PsyI GACNNNGTC 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 2 cut(s) 598, 966
RsaNI GTAC 2 cut(s) 597, 965
RseI CAYNNNNRTG 1 cut(s) 433
SapI GCTCTTC 1 cut(s) 759
SaqAI TTAA 3 cut(s) 249, 581, 815
SatI GCNGC 3 cut(s) 243, 421, 979
Sau3AI GATC 3 cut(s) 355, 693, 803
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 4 cut(s) 391, 557, 615, 914
ScrFI CCNGG 3 cut(s) 81, 624, 708
SduI GDGCHC 1 cut(s) 377
SetI ASST 9 cut(s) 169, 198, 275, 403, 459, 475, 530, 658, 915
SfaNI GCATC 1 cut(s) 8
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 433
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 7 cut(s) 5, 145, 170, 267, 326, 424, 476
SsiI CCGC 1 cut(s) 420
SspI AATATT 2 cut(s) 414, 733
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 3 cut(s) 79, 622, 706
TaaI ACNGT 1 cut(s) 821
TaiI ACGT 1 cut(s) 275
TaqI TCGA 3 cut(s) 12, 696, 800
TaqII GACCGA 1 cut(s) 917
TasI AATT 7 cut(s) 5, 145, 170, 267, 326, 424, 476
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 3 cut(s) 70, 521, 776
Tru1I TTAA 3 cut(s) 249, 581, 815
Tru9I TTAA 3 cut(s) 249, 581, 815
TscAI CASTG 3 cut(s) 352, 607, 826
TseFI GTSAC 2 cut(s) 38, 343
TseI GCWGC 2 cut(s) 242, 978
Tsp45I GTSAC 2 cut(s) 38, 343
TspDTI ATGAA 3 cut(s) 102, 303, 417
TspGWI ACGGA 2 cut(s) 30, 775
TspRI CASTG 3 cut(s) 352, 607, 826
Tth111I GACNNNGTC 1 cut(s) 904
VneI GTGCAC 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 4 cut(s) 5, 145, 170, 476
XmiI GTMKAC 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.