RchiOBHm_Chr6g0296691

DNA topoisomerase 2-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003647
Physical Location & Seq
Reverse (-)
58438413 .. 58440575
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26631

Sequence Viewer

Length: 636 bp
ATGCTCCAAACGCCGCCGTTTAAGGGCGCCAATGTCTTCATGTCGCGGAAGCTGGTCCCGCCGGAGATTTTCGATGCGCTTCACGACGCTCTCAAGCTCAACGGCGCCAATGTCGTCCTCTGCTCCGACCCCTCGCGCACCGGCCCCGACGACTACCACGTCATCGCTTCCTCCGATCACGAAAAGTTTGAGGATCTCAGAGCCAAGGGTTGCCAACTGCTAGGTCCTCAATGCGTGTTTTCTTGTGCAAACGAGCATAGGTCGCTGCCTAAACAAAAGTTTACTTGTTGCTTAGCATTGGATGGTGTCAATGTACTTGCCTCTGGTTTTGAGGCAGATGAAAAGGTTAAGATAAAGAACTTAGTAACATCAATGGGGGGAGTTTTACATACTAAAACATCTTTGGATGTTAGCTTTGTCATTGCGAAGAATGTCTTGGCTGCAAAATACAAGGCAAGTAACTATACTTGGGCTGTAAATACATTGAAGAAGCCAGTTCTTACTATTAATTGGTTATATCAATGCTGCAATGAACATCGCATTGTTCCTCAAGAATCCTTTAGGGTTCTTCCTGTTTCTGGTTTGACGATCTGTGTTACCAGAATTCCATCAGGTTTATTTACTGTTCTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000785 GO:0001932 GO:0001934 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006275 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009893 GO:0009987 GO:0010556 GO:0010562 GO:0010604 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022402 GO:0022607 GO:0030174 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031570 GO:0032268 GO:0032270 GO:0033036 GO:0033135 GO:0033138 GO:0033260 GO:0033365 GO:0033554 GO:0033674 GO:0034502 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0035561 GO:0035563 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044786 GO:0045786 GO:0045859 GO:0045860 GO:0045937 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051098 GO:0051099 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051641 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070727 GO:0071163 GO:0071165 GO:0071168 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 PTCB-BRCT

Protein Analysis

211

Amino Acids

23.21

Weight (kDa)

8.71

Isoelectric Point (pI)

27.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTCB-BRCT PF12738 105 - 152 8e-06 twin BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccB1I GGYRCC 2 cut(s) 26, 104
AccII CGCG 2 cut(s) 46, 136
AciI CCGC 3 cut(s) 14, 46, 59
AclWI GGATC 1 cut(s) 201
AcsI RAATTY 1 cut(s) 603
AcyI GRCGYC 2 cut(s) 27, 105
AfaI GTAC 1 cut(s) 315
AfiI CCNNNNNNNGG 2 cut(s) 23, 578
AgsI TTSAA 1 cut(s) 487
AjiI CACGTC 1 cut(s) 160
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AluBI AGCT 3 cut(s) 52, 97, 414
AluI AGCT 3 cut(s) 52, 97, 414
AlwI GGATC 1 cut(s) 201
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 3 cut(s) 265, 440, 525
ApoI RAATTY 1 cut(s) 603
AseI ATTAAT 1 cut(s) 507
AspLEI GCGC 4 cut(s) 29, 79, 107, 138
AspS9I GGNCC 3 cut(s) 55, 143, 224
AvaII GGWCC 2 cut(s) 55, 224
BanI GGYRCC 2 cut(s) 26, 104
BbsI GAAGAC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 252, 427, 512
BccI CCATC 2 cut(s) 296, 616
BceAI ACGGC 1 cut(s) 118
BfaI CTAG 1 cut(s) 221
BfoI RGCGCY 2 cut(s) 30, 108
BisI GCNGC 4 cut(s) 14, 266, 441, 526
BlpI GCTNAGC 1 cut(s) 292
BlsI GCNGC 4 cut(s) 15, 267, 442, 527
Bme18I GGWCC 2 cut(s) 55, 224
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 3 cut(s) 55, 143, 224
BmiI GGNNCC 4 cut(s) 28, 57, 106, 145
BmsI GCATC 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 28
Bpu1102I GCTNAGC 1 cut(s) 292
BpuEI CTTGAG 2 cut(s) 77, 534
BsaHI GRCGYC 2 cut(s) 27, 105
BsaJI CCNNGG 1 cut(s) 204
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 578
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 494
Bse3DI GCAATG 2 cut(s) 420, 535
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 2 cut(s) 307, 412
BseLI CCNNNNNNNGG 2 cut(s) 23, 578
BseMI GCAATG 2 cut(s) 420, 535
BseMII CTCAG 1 cut(s) 211
BseNI ACTGG 1 cut(s) 494
BseXI GCAGC 3 cut(s) 252, 427, 512
Bsh1236I CGCG 2 cut(s) 46, 136
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 2 cut(s) 26, 104
BsiSI CCGG 2 cut(s) 62, 141
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 2 cut(s) 23, 578
BsmFI GGGAC 1 cut(s) 41
BsnI GGCC 1 cut(s) 144
Bsp143I GATC 3 cut(s) 175, 193, 588
Bsp1720I GCTNAGC 1 cut(s) 292
BspACI CCGC 3 cut(s) 14, 46, 59
BspANI GGCC 1 cut(s) 144
BspCNI CTCAG 1 cut(s) 210
BspFNI CGCG 2 cut(s) 46, 136
BspLI GGNNCC 4 cut(s) 28, 57, 106, 145
BspPI GGATC 1 cut(s) 201
BspT107I GGYRCC 2 cut(s) 26, 104
BsrDI GCAATG 2 cut(s) 420, 535
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 3 cut(s) 175, 193, 588
BssNI GRCGYC 2 cut(s) 27, 105
BssT1I CCWWGG 1 cut(s) 204
Bst4CI ACNGT 1 cut(s) 625
BstACI GRCGYC 2 cut(s) 27, 105
BstDEI CTNAG 3 cut(s) 197, 292, 361
BstF5I GGATG 2 cut(s) 307, 412
BstFNI CGCG 2 cut(s) 46, 136
BstH2I RGCGCY 2 cut(s) 30, 108
BstHHI GCGC 4 cut(s) 29, 79, 107, 138
BstKTI GATC 3 cut(s) 178, 196, 591
BstMBI GATC 3 cut(s) 175, 193, 588
BstMWI GCNNNNNNNGC 3 cut(s) 10, 58, 262
BstUI CGCG 2 cut(s) 46, 136
BstV1I GCAGC 3 cut(s) 252, 427, 512
BstV2I GAAGAC 1 cut(s) 28
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 1 cut(s) 144
BtgZI GCGATG 2 cut(s) 148, 521
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 2 cut(s) 307, 412
CfoI GCGC 4 cut(s) 29, 79, 107, 138
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 55, 143, 224
CseI GACGC 1 cut(s) 95
Csp6I GTAC 1 cut(s) 314
CviAII CATG 1 cut(s) 40
CviJI RGCY 8 cut(s) 52, 97, 144, 203, 414, 440, 473, 493
CviKI_1 RGCY 8 cut(s) 52, 97, 144, 203, 414, 440, 473, 493
CviQI GTAC 1 cut(s) 314
DdeI CTNAG 3 cut(s) 197, 292, 361
DinI GGCGCC 2 cut(s) 28, 106
DpnI GATC 3 cut(s) 177, 195, 590
DpnII GATC 3 cut(s) 175, 193, 588
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 55, 224
EcoO109I RGGNCCY 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 204
EgeI GGCGCC 2 cut(s) 28, 106
EheI GGCGCC 2 cut(s) 28, 106
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 1 cut(s) 43
FaiI YATR 6 cut(s) 41, 258, 390, 465, 517, 634
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 1 cut(s) 41
FatI CATG 1 cut(s) 39
FauI CCCGC 1 cut(s) 66
Fnu4HI GCNGC 4 cut(s) 14, 266, 441, 526
FokI GGATG 2 cut(s) 314, 419
Fsp4HI GCNGC 4 cut(s) 14, 266, 441, 526
FspBI CTAG 1 cut(s) 221
GlaI GCGC 4 cut(s) 28, 78, 106, 137
GluI GCNGC 4 cut(s) 14, 266, 441, 526
HaeII RGCGCY 2 cut(s) 30, 108
HaeIII GGCC 1 cut(s) 144
HapII CCGG 2 cut(s) 62, 141
HgaI GACGC 1 cut(s) 95
HhaI GCGC 4 cut(s) 29, 79, 107, 138
Hin1I GRCGYC 2 cut(s) 27, 105
Hin1II CATG 1 cut(s) 43
Hin6I GCGC 4 cut(s) 27, 77, 105, 136
HinP1I GCGC 4 cut(s) 27, 77, 105, 136
HinfI GANTC 1 cut(s) 554
HpaII CCGG 2 cut(s) 62, 141
Hpy166II GTNNAC 1 cut(s) 282
Hpy188I TCNGA 3 cut(s) 127, 175, 200
Hpy188III TCNNGA 3 cut(s) 83, 179, 551
Hpy8I GTNNAC 1 cut(s) 282
Hpy99I CGWCG 2 cut(s) 89, 152
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 3 cut(s) 248, 443, 528
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 58, 262
HpyF3I CTNAG 3 cut(s) 197, 292, 361
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 2 cut(s) 27, 105
Hsp92II CATG 1 cut(s) 43
HspAI GCGC 4 cut(s) 27, 77, 105, 136
KasI GGCGCC 2 cut(s) 26, 104
Kzo9I GATC 3 cut(s) 175, 193, 588
LmnI GCTCC 2 cut(s) 9, 128
LpnPI CCDG 9 cut(s) 38, 75, 154, 309, 507, 564, 585, 597, 613
Lsp1109I GCAGC 3 cut(s) 252, 427, 512
LweI GCATC 1 cut(s) 64
MaeI CTAG 1 cut(s) 221
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 3 cut(s) 364, 458, 595
MalI GATC 3 cut(s) 177, 195, 590
MboI GATC 3 cut(s) 175, 193, 588
MboII GAAGA 5 cut(s) 28, 439, 499, 560, 620
MflI RGATCY 1 cut(s) 193
MluCI AATT 2 cut(s) 508, 603
Mly113I GGCGCC 2 cut(s) 27, 105
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 8 cut(s) 128, 142, 181, 184, 237, 325, 331, 558
MseI TTAA 3 cut(s) 21, 348, 507
MspI CCGG 2 cut(s) 62, 141
MvnI CGCG 2 cut(s) 46, 136
MwoI GCNNNNNNNGC 3 cut(s) 10, 58, 262
NarI GGCGCC 2 cut(s) 27, 105
NdeII GATC 3 cut(s) 175, 193, 588
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 4 cut(s) 28, 57, 106, 145
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 171
PfeI GAWTC 1 cut(s) 554
PkrI GCNGC 4 cut(s) 15, 267, 442, 527
PluTI GGCGCC 2 cut(s) 30, 108
PpuMI RGGWCCY 1 cut(s) 224
PshBI ATTAAT 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 224
PspN4I GGNNCC 4 cut(s) 28, 57, 106, 145
PspPI GGNCC 3 cut(s) 55, 143, 224
PspPPI RGGWCCY 1 cut(s) 224
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 1 cut(s) 315
RsaNI GTAC 1 cut(s) 314
SaqAI TTAA 3 cut(s) 21, 348, 507
SatI GCNGC 4 cut(s) 14, 266, 441, 526
Sau3AI GATC 3 cut(s) 175, 193, 588
Sau96I GGNCC 3 cut(s) 55, 143, 224
SetI ASST 8 cut(s) 54, 99, 162, 226, 263, 348, 416, 616
SfaNI GCATC 1 cut(s) 64
SfoI GGCGCC 2 cut(s) 28, 106
SinI GGWCC 2 cut(s) 55, 224
SmlI CTYRAG 2 cut(s) 92, 549
SmoI CTYRAG 2 cut(s) 92, 549
Sse9I AATT 2 cut(s) 508, 603
SsiI CCGC 3 cut(s) 14, 46, 59
SspDI GGCGCC 2 cut(s) 26, 104
SspMI CTAG 1 cut(s) 221
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 1 cut(s) 625
TaiI ACGT 1 cut(s) 162
TaqI TCGA 1 cut(s) 72
TasI AATT 2 cut(s) 508, 603
TatI WGTACW 1 cut(s) 313
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 3 cut(s) 21, 348, 507
Tru9I TTAA 3 cut(s) 21, 348, 507
TseI GCWGC 3 cut(s) 265, 440, 525
TspDTI ATGAA 3 cut(s) 28, 354, 546
VpaK11BI GGWCC 2 cut(s) 55, 224
VspI ATTAAT 1 cut(s) 507
XapI RAATTY 1 cut(s) 603
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.