FvH4_2g33540

F-box-like WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003410
Physical Location & Seq
Reverse (-)
25127888 .. 25131598
3711 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33540.t4

Sequence Viewer

Length: 1641 bp
ATGATCTCCCTCAACTCCACCGAATTGAACTACCTCGTCTTTCGTTACCTCCACGAATCAGGCTTCACTCATTCCGCTTATGCTTTCGGATTCGAGGCCGGAGTCAACAAGAGCACCGTGGATGGTAATCTAGTTCCGCCGGGCGCACTAGTAACTTTTCTACAGAAAGGCCTTCAGTTTCTCGAGCTTGAGGCGGACTTAGGCAATAGTAGTGATACGGATACGGAGGATGACTTTTCGTTTCTGCAACCGTTGGATTTTATCACAAAAGATGCGTATCAGTTACAGAAAATTGTTAAGGAAAAGAAGAATAGGATGAACGCTGCTAACAATGAGGCCAGGCAGGAAAGAGAAAGGGAAGTAGAGGTGGAGAGAAAGGAAGAGGTCCAAGGCGATAAGGCGGAGAAGGATAGGGAACAGGAAATTGGTAAAGAGAAGGAGGAGTCGAAGCAGGATCACATACAGATGGAGAAACTGGAGCCTGAAGAAAATGGAGCTGGTGGAGGGGTGGAGCCAATGGAGACCTCCCCAAGCTCAACATCCGCGCCTAGTGAAATTCCTACTAGTGATGTCATAGTTCTAGAAGGCCATACCTCAGAGGTTTTTGCTTGTGCGTGGAGTCCATCAGGTTCGCTTCTTGCATCTGGTTCTGGAGATTCAACAGCTCGAATTTGGACCATTGCCGGTGGAAGTTGTAGATCTAGCATGCAAAATGAACCTGTACATGTTTCTGTGTTAAAGCATTTCAAAGGGAGAGCCAATGAGAAAAGCAAGGATGTGACTACGCTTGATTGGAATGATGATGGAACGTTGCTAGCAACAGGTTCCTATGATGGCCAAGCAAGAATATGGGGAAGAGACGGAGAGTTAAGGACTACTTTGATCAGACATAAAGGGCCAATCTTTTCTCTGAAGTGGAACAAAGAAGGCGATTATCTTCTGAGTGGAAGTGTGGATAGAACTGCAATTGTGTGGGAGTTAAAGACTGGCGAATGGAAACAACAATTTGAATTTCATTCAGCTCCAACTCTGGATGTTGATTGGCGAGACAATACTTCTTTTGCAACATGCTCTACTGATGGTACAATACATGTCTGCAAGGTCGGAGAGAACCAACCAATCAAAACTTTCTTAGGCCATCAGGGTGAAGTAAATGCTATTAAGTGGGATCCGACTGGCTCATTATTGGCTTCTTGCTCTGATGATCATACAGCAAAGATATGGAGTATGAAGCAAGACAACTATTTGCATGATTTGAAGGAACATGTTAAGGAGATATATACAATCCGGTGGAGTCCTACAGGACCTGGTTCACATAATCCAAATCAACAATTAGTGATAGCAAGCGCTTCCTTTGATTCACACGTAAAACTCTGGGATGTGGAACGAGGAACACTTATCTACAACTTATATGGCCACAGGGATCCGGTATACTCTGTTGCATTCAGCCCTAATGGTGAGTACTTGGCTAGTGGATCTATGGACAAATGCATGCACATATGGTCTGTGAAGGAAGGCAAACTAGTAAAAACATACACCGGAAATGGGGGAATATTTGAAGTTTGTTGGAACAAAGATGGCGATAAGATAGCTGCTTGTTTTGCTAACAATAATGTATGTATTGTGGATTTCCGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0001067 GO:0002021 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006508 GO:0006511 GO:0006629 GO:0006807 GO:0006996 GO:0007275 GO:0008013 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009888 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010647 GO:0015630 GO:0016042 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0017053 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0023051 GO:0023056 GO:0030111 GO:0030154 GO:0030163 GO:0030177 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031667 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0035264 GO:0035601 GO:0036211 GO:0040007 GO:0042393 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044212 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0045444 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0047485 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050872 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051603 GO:0060255 GO:0060612 GO:0060613 GO:0060828 GO:0061448 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090207 GO:0090263 GO:0097159 GO:0098732 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140110 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04310 map04013 map04310
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

547

Amino Acids

60.83

Weight (kDa)

5.15

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 8 - 32 1.7e-06 LisH
WD40_WDHD1_1st PF24817 188 - 240 1e-05 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 189 - 286 6.2e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 191 - 284 3e-15 WDR5 beta-propeller domain
WD40 PF00400 191 - 225 4.1e-10 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 191 - 238 2.1e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 192 - 236 3.1e-06 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 193 - 286 1.9e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 195 - 329 4.7e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 196 - 237 3.2e-07 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 252 - 351 2.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 254 - 365 1.2e-20 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 254 - 346 2.2e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 254 - 332 3.8e-09 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 256 - 470 8.3e-08 WDR36/Utp21 first beta-propeller
Beta-prop_Aladin PF25460 256 - 331 2.7e-06 Aladin seven-bladed propeller
WD40 PF00400 257 - 284 3.3e-07 WD domain, G-beta repeat
EIF3I PF24805 257 - 360 1.3e-09 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 257 - 435 3e-12 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 257 - 339 9.4e-11 WDHD1 first WD40 domain
WDR55 PF24796 259 - 416 1.2e-11 WDR55
WD40_MABP1-WDR62_2nd PF24782 260 - 432 8.9e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd PF25172 260 - 367 6.1e-09 WDR3 second beta-propeller domain
WD40 PF00400 289 - 325 1.3e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 292 - 460 8.8e-13 TEP-1 second beta-propeller
Beta-prop_WDR35_TULP_N PF24797 292 - 447 6.5e-06 WDR35/TULP4 N-terminal
Beta-prop_CAF1B_HIR1 PF24105 294 - 372 2.9e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 294 - 412 7e-11 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 297 - 378 2.6e-11 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 297 - 470 1.3e-26 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 311 - 416 5.5e-11 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 344 - 536 2.3e-22 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 345 - 532 2.2e-24 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 349 - 474 3.3e-22 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 350 - 433 1.1e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 350 - 474 3.6e-26 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 352 - 536 7.3e-07 MABP1/WDR62 second WD40 domain
ANAPC4_WD40 PF12894 355 - 432 7.5e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40 PF00400 372 - 409 4.1e-11 WD domain, G-beta repeat
EIF3I PF24805 377 - 470 2e-07 EIF3I
Beta-prop_WDR3_2nd PF25172 377 - 495 6.8e-07 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 379 - 467 7.6e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 380 - 472 1.8e-16 THOC3 beta-propeller domain
Beta-prop_THOC3 PF25174 447 - 536 6.2e-13 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 474 - 535 5.6e-09 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1431
AccII CGCG 1 cut(s) 545
AciI CCGC 5 cut(s) 75, 137, 194, 401, 543
AclI AACGTT 1 cut(s) 809
AclWI GGATC 6 cut(s) 462, 1163, 1176, 1418, 1431, 1483
AcoI YGGCCR 2 cut(s) 835, 1414
AcsI RAATTY 3 cut(s) 555, 669, 1010
AcuI CTGAAG 3 cut(s) 158, 504, 932
AfaI GTAC 3 cut(s) 723, 1084, 1463
AfeI AGCGCT 1 cut(s) 1348
AfiI CCNNNNNNNGG 1 cut(s) 1545
AflIII ACRYGT 4 cut(s) 724, 1090, 1264, 1363
AgsI TTSAA 6 cut(s) 28, 660, 748, 1010, 1258, 1559
AhlI ACTAGT 3 cut(s) 148, 563, 1522
AjnI CCWGG 2 cut(s) 338, 1306
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
AluBI AGCT 6 cut(s) 187, 497, 534, 665, 1022, 1592
AluI AGCT 6 cut(s) 187, 497, 534, 665, 1022, 1592
Alw21I GWGCWC 1 cut(s) 116
Alw26I GTCTC 3 cut(s) 515, 852, 1041
AlwI GGATC 6 cut(s) 462, 1163, 1176, 1418, 1431, 1483
AlwNI CAGNNNCTG 1 cut(s) 1307
Ama87I CYCGRG 1 cut(s) 182
Aor51HI AGCGCT 1 cut(s) 1348
AoxI GGCC 8 cut(s) 96, 169, 336, 586, 835, 896, 1135, 1414
ApeKI GCWGC 2 cut(s) 323, 1592
ApoI RAATTY 3 cut(s) 555, 669, 1010
AspLEI GCGC 3 cut(s) 146, 547, 1349
AspS9I GGNCC 4 cut(s) 385, 675, 896, 1304
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 2 cut(s) 1157, 1469
AsuNHI GCTAGC 1 cut(s) 814
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 3 cut(s) 385, 675, 1304
BalI TGGCCA 2 cut(s) 837, 1416
BamHI GGATCC 2 cut(s) 1168, 1423
Bbv12I GWGCWC 1 cut(s) 116
BbvI GCAGC 2 cut(s) 310, 1579
BccI CCATC 8 cut(s) 116, 460, 631, 797, 827, 1073, 1146, 1571
BciT130I CCWGG 2 cut(s) 340, 1308
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 2 cut(s) 882, 1204
BcnI CCSGG 1 cut(s) 141
BcoDI GTCTC 3 cut(s) 515, 852, 1041
BcuI ACTAGT 3 cut(s) 148, 563, 1522
BfaI CTAG 9 cut(s) 131, 149, 549, 564, 581, 702, 815, 1470, 1523
BfmI CTRYAG 2 cut(s) 161, 1299
BfoI RGCGCY 1 cut(s) 1350
BfuI GTATCC 1 cut(s) 214
BglII AGATCT 1 cut(s) 698
BisI GCNGC 2 cut(s) 324, 1593
BlsI GCNGC 2 cut(s) 325, 1594
BmcAI AGTACT 1 cut(s) 1463
Bme1390I CCNGG 3 cut(s) 141, 340, 1308
Bme18I GGWCC 3 cut(s) 385, 675, 1304
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 4 cut(s) 385, 675, 896, 1304
BmiI GGNNCC 5 cut(s) 480, 513, 826, 1170, 1425
BmrFI CCNGG 3 cut(s) 141, 340, 1308
BmsI GCATC 2 cut(s) 262, 650
BmtI GCTAGC 1 cut(s) 818
BpmI CTGGAG 2 cut(s) 497, 672
BpuEI CTTGAG 1 cut(s) 209
BpuMI CCSGG 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 1366
BsaBI GATNNNNATC 2 cut(s) 126, 276
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 2 cut(s) 117, 388
BsaWI WCCGGW 3 cut(s) 1287, 1426, 1538
Bsc4I CCNNNNNNNGG 1 cut(s) 1545
Bse118I RCCGGY 1 cut(s) 683
Bse1I ACTGG 3 cut(s) 480, 991, 1180
Bse3DI GCAATG 1 cut(s) 678
Bse8I GATNNNNATC 2 cut(s) 126, 276
BseBI CCWGG 2 cut(s) 340, 1308
BseDI CCNNGG 2 cut(s) 117, 388
BseGI GGATG 7 cut(s) 127, 235, 321, 539, 781, 1039, 1384
BseJI GATNNNNATC 2 cut(s) 126, 276
BseLI CCNNNNNNNGG 1 cut(s) 1545
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 2 cut(s) 609, 932
BseNI ACTGG 3 cut(s) 480, 991, 1180
BseRI GAGGAG 1 cut(s) 455
BseXI GCAGC 2 cut(s) 310, 1579
Bsh1236I CGCG 1 cut(s) 545
BshFI GGCC 8 cut(s) 98, 171, 338, 588, 837, 898, 1137, 1416
BsiHKAI GWGCWC 1 cut(s) 116
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 6 cut(s) 99, 140, 684, 1288, 1427, 1539
BslI CCNNNNNNNGG 1 cut(s) 1545
BsmAI GTCTC 3 cut(s) 515, 852, 1041
BsmBI CGTCTC 1 cut(s) 852
BsmI GAATGC 1 cut(s) 1442
BsnI GGCC 8 cut(s) 98, 171, 338, 588, 837, 898, 1137, 1416
Bso31I GGTCTC 1 cut(s) 515
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 116
Bsp1407I TGTACA 1 cut(s) 721
Bsp143I GATC 8 cut(s) 3, 454, 698, 882, 1168, 1204, 1423, 1475
BspACI CCGC 5 cut(s) 75, 137, 194, 401, 543
BspANI GGCC 8 cut(s) 98, 171, 338, 588, 837, 898, 1137, 1416
BspCNI CTCAG 2 cut(s) 608, 933
BspFNI CGCG 1 cut(s) 545
BspLI GGNNCC 5 cut(s) 480, 513, 826, 1170, 1425
BspOI GCTAGC 1 cut(s) 818
BspPI GGATC 6 cut(s) 462, 1163, 1176, 1418, 1431, 1483
BspTNI GGTCTC 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 683
BsrGI TGTACA 1 cut(s) 721
BsrI ACTGG 3 cut(s) 480, 991, 1180
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 2 cut(s) 117, 388
BssMI GATC 8 cut(s) 3, 454, 698, 882, 1168, 1204, 1423, 1475
BssNAI GTATAC 1 cut(s) 1432
BssT1I CCWWGG 1 cut(s) 388
Bst1107I GTATAC 1 cut(s) 1432
Bst2UI CCWGG 2 cut(s) 340, 1308
Bst4CI ACNGT 2 cut(s) 118, 252
Bst6I CTCTTC 2 cut(s) 375, 850
BstAUI TGTACA 1 cut(s) 721
BstBAI YACGTR 1 cut(s) 1366
BstC8I GCNNGC 4 cut(s) 707, 816, 1345, 1493
BstDEI CTNAG 4 cut(s) 199, 595, 941, 1132
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 7 cut(s) 127, 235, 321, 539, 781, 1039, 1384
BstFNI CGCG 1 cut(s) 545
BstH2I RGCGCY 1 cut(s) 1350
BstHHI GCGC 3 cut(s) 146, 547, 1349
BstKTI GATC 8 cut(s) 6, 457, 701, 885, 1171, 1207, 1426, 1478
BstMAI GTCTC 3 cut(s) 515, 852, 1041
BstMBI GATC 8 cut(s) 3, 454, 698, 882, 1168, 1204, 1423, 1475
BstMWI GCNNNNNNNGC 1 cut(s) 1601
BstNI CCWGG 2 cut(s) 340, 1308
BstNSI RCATGY 6 cut(s) 709, 728, 1071, 1094, 1268, 1495
BstSCI CCNGG 3 cut(s) 139, 338, 1306
BstSFI CTRYAG 2 cut(s) 161, 1299
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 2 cut(s) 310, 1579
BstX2I RGATCY 4 cut(s) 698, 1168, 1423, 1475
BstYI RGATCY 4 cut(s) 698, 1168, 1423, 1475
BstZ17I GTATAC 1 cut(s) 1432
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 8 cut(s) 98, 171, 338, 588, 837, 898, 1137, 1416
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 7 cut(s) 127, 235, 321, 539, 781, 1039, 1384
Cac8I GCNNGC 4 cut(s) 707, 816, 1345, 1493
CaiI CAGNNNCTG 1 cut(s) 1307
CfoI GCGC 3 cut(s) 146, 547, 1349
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 4 cut(s) 385, 675, 896, 1304
CsiI ACCWGGT 1 cut(s) 1306
Csp6I GTAC 3 cut(s) 722, 1083, 1462
CviAII CATG 7 cut(s) 706, 725, 1068, 1091, 1250, 1265, 1492
CviQI GTAC 3 cut(s) 722, 1083, 1462
DdeI CTNAG 4 cut(s) 199, 595, 941, 1132
DpnI GATC 8 cut(s) 5, 456, 700, 884, 1170, 1206, 1425, 1477
DpnII GATC 8 cut(s) 3, 454, 698, 882, 1168, 1204, 1423, 1475
EaeI YGGCCR 2 cut(s) 835, 1414
Eam1104I CTCTTC 2 cut(s) 375, 850
EarI CTCTTC 2 cut(s) 375, 850
EciI GGCGGA 3 cut(s) 126, 209, 416
Eco130I CCWWGG 1 cut(s) 388
Eco147I AGGCCT 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 3 cut(s) 385, 675, 1304
Eco47III AGCGCT 1 cut(s) 1348
Eco57I CTGAAG 3 cut(s) 158, 504, 932
Eco88I CYCGRG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 1304
EcoRII CCWGG 2 cut(s) 338, 1306
EcoT14I CCWWGG 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 1493
ErhI CCWWGG 1 cut(s) 388
Esp3I CGTCTC 1 cut(s) 852
FaeI CATG 7 cut(s) 709, 728, 1071, 1094, 1253, 1268, 1495
FalI AAGNNNNNCTT 2 cut(s) 862, 894
FatI CATG 7 cut(s) 705, 724, 1067, 1090, 1249, 1264, 1491
FauNDI CATATG 1 cut(s) 1499
FbaI TGATCA 2 cut(s) 882, 1204
FblI GTMKAC 1 cut(s) 1431
Fnu4HI GCNGC 2 cut(s) 324, 1593
FokI GGATG 7 cut(s) 134, 242, 328, 526, 788, 1046, 1391
Fsp4HI GCNGC 2 cut(s) 324, 1593
FspBI CTAG 9 cut(s) 131, 149, 549, 564, 581, 702, 815, 1470, 1523
GlaI GCGC 3 cut(s) 145, 546, 1348
GluI GCNGC 2 cut(s) 324, 1593
GsuI CTGGAG 2 cut(s) 497, 672
HaeII RGCGCY 1 cut(s) 1350
HaeIII GGCC 8 cut(s) 98, 171, 338, 588, 837, 898, 1137, 1416
HapII CCGG 6 cut(s) 99, 140, 684, 1288, 1427, 1539
HhaI GCGC 3 cut(s) 146, 547, 1349
Hin1II CATG 7 cut(s) 709, 728, 1071, 1094, 1253, 1268, 1495
Hin6I GCGC 3 cut(s) 144, 545, 1347
HinP1I GCGC 3 cut(s) 144, 545, 1347
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 8 cut(s) 56, 90, 102, 443, 619, 656, 1294, 1358
HpaII CCGG 6 cut(s) 99, 140, 684, 1288, 1427, 1539
HphI GGTGA 2 cut(s) 1157, 1469
Hpy166II GTNNAC 3 cut(s) 106, 1313, 1432
Hpy188I TCNGA 9 cut(s) 89, 598, 887, 912, 942, 1106, 1173, 1201, 1634
Hpy188III TCNNGA 4 cut(s) 182, 581, 651, 1031
Hpy8I GTNNAC 3 cut(s) 106, 1313, 1432
HpyAV CCTTC 8 cut(s) 182, 400, 430, 578, 920, 1252, 1504, 1508
HpyCH4III ACNGT 2 cut(s) 118, 252
HpyCH4IV ACGT 2 cut(s) 809, 1365
HpyF10VI GCNNNNNNNGC 1 cut(s) 1601
HpyF3I CTNAG 4 cut(s) 199, 595, 941, 1132
HpySE526I ACGT 2 cut(s) 809, 1365
Hsp92II CATG 7 cut(s) 709, 728, 1071, 1094, 1253, 1268, 1495
HspAI GCGC 3 cut(s) 144, 545, 1347
Ksp22I TGATCA 2 cut(s) 882, 1204
Kzo9I GATC 8 cut(s) 3, 454, 698, 882, 1168, 1204, 1423, 1475
LmnI GCTCC 4 cut(s) 478, 494, 511, 1027
Lsp1109I GCAGC 2 cut(s) 310, 1579
LweI GCATC 2 cut(s) 262, 650
MabI ACCWGGT 1 cut(s) 1306
MaeI CTAG 9 cut(s) 131, 149, 549, 564, 581, 702, 815, 1470, 1523
MaeII ACGT 2 cut(s) 809, 1365
MaeIII GTNAC 4 cut(s) 44, 151, 282, 778
MalI GATC 8 cut(s) 5, 456, 700, 884, 1170, 1206, 1425, 1477
MboI GATC 8 cut(s) 3, 454, 698, 882, 1168, 1204, 1423, 1475
MboII GAAGA 5 cut(s) 319, 392, 497, 867, 929
MfeI CAATTG 1 cut(s) 966
MflI RGATCY 4 cut(s) 698, 1168, 1423, 1475
MhlI GDGCHC 1 cut(s) 116
MlsI TGGCCA 2 cut(s) 837, 1416
MluCI AATT 9 cut(s) 23, 291, 423, 555, 669, 966, 1004, 1010, 1331
MluNI TGGCCA 2 cut(s) 837, 1416
MlyI GAGTC 4 cut(s) 111, 452, 628, 1303
MmeI TCCRAC 5 cut(s) 234, 1049, 1084, 1196, 1547
Mox20I TGGCCA 2 cut(s) 837, 1416
Mph1103I ATGCAT 1 cut(s) 1493
MscI TGGCCA 2 cut(s) 837, 1416
MseI TTAA 6 cut(s) 297, 737, 869, 980, 1161, 1269
MslI CAYNNNNRTG 2 cut(s) 464, 1143
Msp20I TGGCCA 2 cut(s) 837, 1416
MspI CCGG 6 cut(s) 99, 140, 684, 1288, 1427, 1539
MspR9I CCNGG 3 cut(s) 141, 340, 1308
MunI CAATTG 1 cut(s) 966
Mva1269I GAATGC 1 cut(s) 1442
MvaI CCWGG 2 cut(s) 340, 1308
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 1 cut(s) 1601
NciI CCSGG 1 cut(s) 141
NdeI CATATG 1 cut(s) 1499
NdeII GATC 8 cut(s) 3, 454, 698, 882, 1168, 1204, 1423, 1475
NheI GCTAGC 1 cut(s) 814
NlaIII CATG 7 cut(s) 709, 728, 1071, 1094, 1253, 1268, 1495
NlaIV GGNNCC 5 cut(s) 480, 513, 826, 1170, 1425
NmuCI GTSAC 1 cut(s) 778
NsiI ATGCAT 1 cut(s) 1493
NspI RCATGY 6 cut(s) 709, 728, 1071, 1094, 1268, 1495
PaeI GCATGC 2 cut(s) 709, 1495
PaeR7I CTCGAG 1 cut(s) 182
PceI AGGCCT 1 cut(s) 171
PciI ACATGT 3 cut(s) 724, 1090, 1264
PctI GAATGC 1 cut(s) 1442
PfeI GAWTC 4 cut(s) 56, 90, 656, 1358
PkrI GCNGC 2 cut(s) 325, 1594
PleI GAGTC 4 cut(s) 110, 451, 627, 1302
PpsI GAGTC 4 cut(s) 110, 451, 627, 1302
Ppu21I YACGTR 1 cut(s) 1366
PpuMI RGGWCCY 1 cut(s) 1304
PscI ACATGT 3 cut(s) 724, 1090, 1264
Psp1406I AACGTT 1 cut(s) 809
Psp5II RGGWCCY 1 cut(s) 1304
Psp6I CCWGG 2 cut(s) 338, 1306
PspGI CCWGG 2 cut(s) 338, 1306
PspN4I GGNNCC 5 cut(s) 480, 513, 826, 1170, 1425
PspPI GGNCC 4 cut(s) 385, 675, 896, 1304
PspPPI RGGWCCY 1 cut(s) 1304
PstNI CAGNNNCTG 1 cut(s) 1307
PsuI RGATCY 4 cut(s) 698, 1168, 1423, 1475
RsaI GTAC 3 cut(s) 723, 1084, 1463
RsaNI GTAC 3 cut(s) 722, 1083, 1462
RseI CAYNNNNRTG 2 cut(s) 464, 1143
SaqAI TTAA 6 cut(s) 297, 737, 869, 980, 1161, 1269
SatI GCNGC 2 cut(s) 324, 1593
Sau3AI GATC 8 cut(s) 3, 454, 698, 882, 1168, 1204, 1423, 1475
Sau96I GGNCC 4 cut(s) 385, 675, 896, 1304
ScaI AGTACT 1 cut(s) 1463
SchI GAGTC 4 cut(s) 111, 452, 628, 1303
ScrFI CCNGG 3 cut(s) 141, 340, 1308
SduI GDGCHC 1 cut(s) 116
SexAI ACCWGGT 1 cut(s) 1306
SfaNI GCATC 2 cut(s) 262, 650
SfcI CTRYAG 2 cut(s) 161, 1299
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 3 cut(s) 385, 675, 1304
SlaI CTCGAG 1 cut(s) 182
SmiMI CAYNNNNRTG 2 cut(s) 464, 1143
SmlI CTYRAG 2 cut(s) 182, 188
SmoI CTYRAG 2 cut(s) 182, 188
SpeI ACTAGT 3 cut(s) 148, 563, 1522
SphI GCATGC 2 cut(s) 709, 1495
Sse9I AATT 9 cut(s) 23, 291, 423, 555, 669, 966, 1004, 1010, 1331
SseBI AGGCCT 1 cut(s) 171
SsiI CCGC 5 cut(s) 75, 137, 194, 401, 543
SspI AATATT 1 cut(s) 1554
SspMI CTAG 9 cut(s) 131, 149, 549, 564, 581, 702, 815, 1470, 1523
StuI AGGCCT 1 cut(s) 171
StyD4I CCNGG 3 cut(s) 139, 338, 1306
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 118, 252
TaiI ACGT 2 cut(s) 812, 1368
TaqI TCGA 4 cut(s) 93, 183, 446, 667
TasI AATT 9 cut(s) 23, 291, 423, 555, 669, 966, 1004, 1010, 1331
TatI WGTACW 2 cut(s) 721, 1461
TfiI GAWTC 4 cut(s) 56, 90, 656, 1358
Tru1I TTAA 6 cut(s) 297, 737, 869, 980, 1161, 1269
Tru9I TTAA 6 cut(s) 297, 737, 869, 980, 1161, 1269
TseFI GTSAC 1 cut(s) 778
TseI GCWGC 2 cut(s) 323, 1592
Tsp45I GTSAC 1 cut(s) 778
TspDTI ATGAA 4 cut(s) 332, 729, 1004, 1244
TspGWI ACGGA 3 cut(s) 233, 239, 876
VpaK11BI GGWCC 3 cut(s) 385, 675, 1304
XapI RAATTY 3 cut(s) 555, 669, 1010
XbaI TCTAGA 1 cut(s) 580
XceI RCATGY 6 cut(s) 709, 728, 1071, 1094, 1268, 1495
XhoI CTCGAG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 1431
XspI CTAG 9 cut(s) 131, 149, 549, 564, 581, 702, 815, 1470, 1523
ZrmI AGTACT 1 cut(s) 1463
Zsp2I ATGCAT 1 cut(s) 1493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.