Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003410

Physical Location & Seq

Genomic Coordinates Forward (+)

26832964 .. 26841075

Gene Structure 8112 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1290400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGACCTCCATCACGTCCGTCGAGTTGAATTTCCTAGTCTTTCGTTACCTTCAGGAATCAGGCTTCACACACTCAGCTTTTGCTTTAGGATATGAGGCCGGTATCAACAAATGCACAATAGATGGAAATTTGGTTCCACCTGGTGCCCTCATTACATTTATACAAAAAGGACTTCAGTACTTGGAGATGGAAGCTAACTTGAGTAATAGTGATGCAGATTTGGATGAAGATTTCTCATTTTTACAACCTTTAGATCTTATCACGAAGGATGTAAATCAACTAAGGCAAATGATTACAGAGAAAAGGAAGAATTCACAGATGGACAAAGACAAAGATAAAGAGCACGAGTCTTATCAAAAGAAAGATAAAGATAAGGAGCTTGAAAAAGAACATGACGGAGAACGTGCACGAGTAAGAGAGAAAGAAAGAAAAGAAAGGGAGAAAGATTTCGAAAAGGATAGAGAGAGGATAGAAAAGAATAAGGAGCGAGAAAAGCAGCGTGAGGACCGCAATGACAGAGATGTAGTTAGAGATCAGGAAGATAAAGTTAATGTCAGACATGAAGAAAATGGAGCTTCTGGAGAACCAATGGATATTCCTCTTACAGATGTTGAAATCCCTAGTTCTGATGTGACAATTTTGGAGGGGCATACTAATGAGGTATGTGCTTGTGCATGGAGTCCAGCAGGTTCCCTTCTTGCATCAGGGTCTGGAGATTCGACAGCTCGTATTTGGACCATTGCTGATGGAAGCTCCAGATTTAGTTCACAAAATGGTTCCTCAAATGTGTTGGTGCTGAAGCATGTTAAGGGTAGAACAAATGAAAAGAGTAAAGATGTGACGACGCTTGACTGGAATGGAGAGGGAACAATGCTTGCAACCGGGTCGTATGATGGGCAAGCAAGAATTTGGAGTACAAAGGGTGAATTAATTAGTACTTTGAGCAAACATAAGGGACCTATATTCTCTCTAAAGTGGAACAAAAAGGGAGATTATCTTCTTACAGGAAGCTGCGATAAAACTGCAATTGTGTGGGACGTAAGGGCAGAGGAACCGAAACAACAATTTGAATTTCATTCAGGTCCAACTCTTGATGTTGACTGGCGCAACAATGTCTCCTTTGCAATGAGCTCAACAGACAATATGATATATGTCTGCAAGATTGGGGAAAACCACCCTGTTAAAACTTTTGCGGGACATCAGGGAGAAGTTAATTGTGTTAAATGGGATCCAACTGGTTCATTGTTGGCTTCATGTTCTGACGATAGCACTGCAAAGATATGGAGCATGAAGCAGGAAAAATATGTTCATGATTTAAGAGAGCATTCTAAGGAGATATACACTATCAGATGGAGTCCCACTGGACCAGGAACAAACAACCCTAACCAACAGTTAGTCCTAGCTAGTGCATCATTTGACTCCTTTGTGAAGCTATGGGATGTGGAACAAGGAAAGCTTCTCTGCAGCTTGGATGGACACAGGGAACCTGTTTATTCTGTTGCTTTTAGCCCAAATGGTGAGTATTTGGCCAGTGGATCTCTTGATAAATCCATGCATATCTGGTCACTGAAGGAACGCAAGATTGTGAAAACGTACACTGGAAGTGGCGGTATATTTGAAGTCTGCTGGAACAAGGAAGGCGACAAACTTGCTGCATGTTTTGCCAACAACACAGTGTGCGTCTTGGATTTTAGAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000151 GO:0003674 GO:0003712 GO:0003714 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006950 GO:0006996 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031461 GO:0031974 GO:0031981 GO:0032991 GO:0035601 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0080090 GO:0098732 GO:0140110 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04013 ko04310 map04013 map04310

Pfam Domains

Protein Families

LisH WD40

Protein Analysis

567

Amino Acids

63.85

Weight (kDa)

5.49

Isoelectric Point (pI)

30.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH	PF08513	8 - 33	9.7e-08	LisH
WD40_WDHD1_1st	PF24817	192 - 252	3.8e-06	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	199 - 258	3.6e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	210 - 251	3.1e-08	WDR5 beta-propeller domain
WD40	PF00400	211 - 245	6.9e-10	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	211 - 255	1e-05	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	212 - 319	1.1e-09	CAF1B/HIR1 beta-propeller domain
Beta-prop_EML	PF23409	213 - 382	7.6e-11	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3	PF25174	217 - 253	1.6e-06	THOC3 beta-propeller domain
Beta-prop_IFT140_1st	PF23383	268 - 347	3.1e-06	IFT140 first beta-propeller
Beta-prop_EML_2	PF23414	268 - 352	6.2e-08	Echinoderm microtubule-associated protein second beta-propeller
ANAPC4_WD40	PF12894	273 - 328	7.5e-06	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR5	PF25175	273 - 370	2.8e-17	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	273 - 367	7.9e-16	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	273 - 353	8.1e-11	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	274 - 522	3.1e-12	WDR36/Utp21 first beta-propeller
Beta-prop_THOC3	PF25174	277 - 513	2.1e-47	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	277 - 434	2.4e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	277 - 455	3.4e-12	WDR36/Utp21 second beta-propeller domain
EIF3I	PF24805	277 - 382	1e-10	EIF3I
NBCH_WD40	PF20426	277 - 440	6.7e-07	Neurobeachin beta propeller domain
WD40_WDHD1_1st	PF24817	277 - 358	2.6e-11	WDHD1 first WD40 domain
WD40	PF00400	277 - 305	5.9e-06	WD domain, G-beta repeat
WDR55	PF24796	278 - 445	2.3e-10	WDR55
Beta-prop_WDR3_2nd	PF25172	280 - 384	7.4e-06	WDR3 second beta-propeller domain
WD40	PF00400	308 - 346	4e-08	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	311 - 490	3.7e-27	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	312 - 480	3.4e-12	TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	313 - 391	1.2e-06	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	323 - 561	1.3e-38	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	334 - 453	9e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	334 - 517	7.6e-10	MABP1/WDR62 second WD40 domain
Beta-prop_WDR19_1st	PF23389	367 - 524	4.8e-06	WDR19 first beta-propeller
Beta-prop_WDR3_1st	PF25173	370 - 523	2.1e-33	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	373 - 503	5.2e-29	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	374 - 559	7.5e-29	WDHD1 first WD40 domain
ANAPC4_WD40	PF12894	375 - 452	3.7e-08	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR3_2nd	PF25172	392 - 523	1.8e-14	WDR3 second beta-propeller domain
WD40	PF00400	393 - 429	5.4e-12	WD domain, G-beta repeat
Beta-prop_EML	PF23409	397 - 531	2e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	397 - 532	1.2e-17	CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin	PF25460	403 - 455	2.2e-06	Aladin seven-bladed propeller
WDR90_beta-prop_4th	PF23342	404 - 516	6.6e-09	WDR90, 4th beta-propeller
WD40_MABP1-WDR62_1st	PF24780	410 - 543	6.3e-07	MABP1/WDR62 first WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	410 - 527	5.9e-08	WDR90/POC16, second beta-propeller
Beta-prop_EML_2	PF23414	460 - 563	4e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_IFT122_1st	PF23381	463 - 556	1.3e-11	IFT122 first beta-propeller
Beta-prop_WDR3_1st	PF25173	463 - 560	1e-16	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	466 - 565	4.7e-20	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	466 - 564	1.3e-10	CDC20/Fizzy WD40 domain
EIF3I	PF24805	466 - 555	6.7e-08	EIF3I
Beta-prop_TEP1_2nd	PF25047	466 - 526	8.3e-07	TEP-1 second beta-propeller
WD40_Gbeta	PF25391	469 - 565	1.9e-09	G protein beta WD-40 repeat protein
WD40	PF00400	484 - 522	2.8e-11	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	490 - 564	6.8e-13	WDR5 beta-propeller domain
Beta-prop_Aladin	PF25460	494 - 561	3.5e-07	Aladin seven-bladed propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67320
fragaria_vesca	FvH4_1g16940 FvH4_2g33540 FvH4_2g33540 FvH4_2g33540 FvH4_2g33540 FvH4_2g33540
malus_domestica	MD02G1179800.v1.1 MD15G1075300.v1.1 MD15G1290400.v1.1
prunus_persica	Prupe.1G428200_v2.0.a1 Prupe.1G428200_v2.0.a1 Prupe.6G226500_v2.0.a1 Prupe.6G226500_v2.0.a1
pyrus_communis	pycom02g14570 pycom15g07030 pycom15g25410
rosa_chinensis	RchiOBHm_Chr2g0107021 RchiOBHm_Chr6g0308951
rosa_laevigata	RLG00000010601 RLG00000017550
rosa_multiflora	Rmu_sc0007106.1_g000002 Rmu_sc0030997.1_g000001
rosa_roxburghii	Rroxscaffold_2G00136680 Rroxscaffold_7G00159780
rosa_rugosa	Rorug02G0140000
rosa_samantha	Rh2AG190800 Rh2BG202300 Rh2CG195500 Rh2DG197800 Rh6AG481200 Rh6BG490300 Rh6CG495600 Rh6DG481500
rosa_wichuraiana	Rw2G015040 Rw6G041920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	677
AccB1I	GGYRCC	1 cut(s)	143
AciI	CCGC	3 cut(s)	508, 1193, 1608
AclWI	GGATC	3 cut(s)	1223, 1236, 1543
AcoI	YGGCCR	1 cut(s)	1527
AcsI	RAATTY	5 cut(s)	28, 127, 310, 906, 1070
AcuI	CTGAAG	4 cut(s)	35, 158, 818, 1589
AdeI	CACNNNGTG	2 cut(s)	143, 1677
AfaI	GTAC	4 cut(s)	179, 916, 937, 1595
AgsI	TTSAA	5 cut(s)	28, 383, 614, 1070, 1619
AjiI	CACGTC	1 cut(s)	15
AjnI	CCWGG	2 cut(s)	139, 1366
AloI	GAACNNNNNNTCC	2 cut(s)	117, 149
Alw21I	GWGCWC	3 cut(s)	345, 409, 1133
Alw26I	GTCTC	1 cut(s)	1120
Alw44I	GTGCAC	1 cut(s)	405
AlwI	GGATC	3 cut(s)	1223, 1236, 1543
AlwNI	CAGNNNCTG	1 cut(s)	710
AoxI	GGCC	2 cut(s)	96, 1527
ApaLI	GTGCAC	1 cut(s)	405
ApeKI	GCWGC	4 cut(s)	496, 1011, 1464, 1652
ApoI	RAATTY	5 cut(s)	28, 127, 310, 906, 1070
AseI	ATTAAT	1 cut(s)	929
Asp700I	GAANNNNTTC	1 cut(s)	446
AspLEI	GCGC	1 cut(s)	1107
AspS9I	GGNCC	5 cut(s)	505, 735, 956, 1082, 1364
AsuC2I	CCSGG	1 cut(s)	883
AsuHPI	GGTGA	2 cut(s)	935, 1529
AsuII	TTCGAA	1 cut(s)	450
AvaII	GGWCC	5 cut(s)	505, 735, 956, 1082, 1364
BaeGI	GKGCMC	2 cut(s)	148, 409
BalI	TGGCCA	1 cut(s)	1529
BamHI	GGATCC	1 cut(s)	1228
BanI	GGYRCC	1 cut(s)	143
BanII	GRGCYC	1 cut(s)	1133
BauI	CACGAG	2 cut(s)	344, 408
Bbv12I	GWGCWC	3 cut(s)	345, 409, 1133
BbvI	GCAGC	4 cut(s)	508, 998, 1476, 1639
BccI	CCATC	8 cut(s)	17, 116, 181, 313, 740, 887, 1344, 1466
BcgI	CGANNNNNNTGC	8 cut(s)	867, 901, 1004, 1038, 1253, 1287, 1631, 1665
BciT130I	CCWGG	2 cut(s)	141, 1368
BcnI	CCSGG	1 cut(s)	883
BcoDI	GTCTC	1 cut(s)	1120
BfaI	CTAG	4 cut(s)	35, 621, 1400, 1404
BfmI	CTRYAG	1 cut(s)	1462
BfuAI	ACCTGC	1 cut(s)	677
BglII	AGATCT	1 cut(s)	253
BisI	GCNGC	4 cut(s)	497, 1012, 1465, 1653
BlsI	GCNGC	4 cut(s)	498, 1013, 1466, 1654
BmcAI	AGTACT	2 cut(s)	179, 937
Bme1390I	CCNGG	3 cut(s)	141, 883, 1368
Bme18I	GGWCC	5 cut(s)	505, 735, 956, 1082, 1364
BmgBI	CACGTC	1 cut(s)	15
BmgT120I	GGNCC	5 cut(s)	505, 735, 956, 1082, 1364
BmiI	GGNNCC	8 cut(s)	135, 145, 691, 778, 957, 1053, 1230, 1485
BmrFI	CCNGG	3 cut(s)	141, 883, 1368
BmsI	GCATC	3 cut(s)	202, 710, 1418
BpmI	CTGGAG	3 cut(s)	600, 732, 739
Bpu14I	TTCGAA	1 cut(s)	450
BpuEI	CTTGAG	1 cut(s)	220
BpuMI	CCSGG	1 cut(s)	883
BsaBI	GATNNNNATC	1 cut(s)	273
BsaXI	ACNNNNNCTCC	2 cut(s)	1100, 1130
Bse118I	RCCGGY	1 cut(s)	98
Bse1I	ACTGG	6 cut(s)	857, 1106, 1240, 1366, 1530, 1603
Bse3DI	GCAATG	3 cut(s)	517, 738, 1131
Bse8I	GATNNNNATC	1 cut(s)	273
BseBI	CCWGG	2 cut(s)	141, 1368
BseGI	GGATG	4 cut(s)	229, 274, 1444, 1477
BseJI	GATNNNNATC	1 cut(s)	273
BseMI	GCAATG	3 cut(s)	517, 738, 1131
BseMII	CTCAG	1 cut(s)	87
BseNI	ACTGG	6 cut(s)	857, 1106, 1240, 1366, 1530, 1603
BseSI	GKGCMC	2 cut(s)	148, 409
BseXI	GCAGC	4 cut(s)	508, 998, 1476, 1639
BshFI	GGCC	2 cut(s)	98, 1529
BshNI	GGYRCC	1 cut(s)	143
BsiHKAI	GWGCWC	3 cut(s)	345, 409, 1133
BsiSI	CCGG	2 cut(s)	99, 882
BslFI	GGGAC	4 cut(s)	969, 1049, 1209, 1341
BsmAI	GTCTC	1 cut(s)	1120
BsmFI	GGGAC	4 cut(s)	969, 1049, 1209, 1341
BsmI	GAATGC	1 cut(s)	1324
BsnI	GGCC	2 cut(s)	98, 1529
Bsp119I	TTCGAA	1 cut(s)	450
Bsp1286I	GDGCHC	4 cut(s)	148, 345, 409, 1133
Bsp143I	GATC	4 cut(s)	253, 532, 1228, 1535
BspACI	CCGC	3 cut(s)	508, 1193, 1608
BspANI	GGCC	2 cut(s)	98, 1529
BspCNI	CTCAG	1 cut(s)	86
BspHI	TCATGA	1 cut(s)	1309
BspLI	GGNNCC	8 cut(s)	135, 145, 691, 778, 957, 1053, 1230, 1485
BspMAI	CTGCAG	1 cut(s)	1466
BspMI	ACCTGC	1 cut(s)	677
BspPI	GGATC	3 cut(s)	1223, 1236, 1543
BspT104I	TTCGAA	1 cut(s)	450
BspT107I	GGYRCC	1 cut(s)	143
BsrDI	GCAATG	3 cut(s)	517, 738, 1131
BsrFI	RCCGGY	1 cut(s)	98
BsrI	ACTGG	6 cut(s)	857, 1106, 1240, 1366, 1530, 1603
BssAI	RCCGGY	1 cut(s)	98
BssMI	GATC	4 cut(s)	253, 532, 1228, 1535
BssSI	CACGAG	2 cut(s)	344, 408
Bst2BI	CACGAG	2 cut(s)	344, 408
Bst2UI	CCWGG	2 cut(s)	141, 1368
Bst4CI	ACNGT	2 cut(s)	1392, 1675
BstAPI	GCANNNNNTGC	1 cut(s)	1661
BstBI	TTCGAA	1 cut(s)	450
BstC8I	GCNNGC	2 cut(s)	876, 900
BstDEI	CTNAG	3 cut(s)	73, 281, 1329
BstF5I	GGATG	4 cut(s)	229, 274, 1444, 1477
BstHHI	GCGC	1 cut(s)	1107
BstKTI	GATC	4 cut(s)	256, 535, 1231, 1538
BstMAI	GTCTC	1 cut(s)	1120
BstMBI	GATC	4 cut(s)	253, 532, 1228, 1535
BstMWI	GCNNNNNNNGC	2 cut(s)	493, 1661
BstNI	CCWGG	2 cut(s)	141, 1368
BstNSI	RCATGY	2 cut(s)	806, 1659
BstSCI	CCNGG	3 cut(s)	139, 881, 1366
BstSFI	CTRYAG	1 cut(s)	1462
BstSLI	GKGCMC	2 cut(s)	148, 409
BstV1I	GCAGC	4 cut(s)	508, 998, 1476, 1639
BstX2I	RGATCY	3 cut(s)	253, 1228, 1535
BstYI	RGATCY	3 cut(s)	253, 1228, 1535
BsuRI	GGCC	2 cut(s)	98, 1529
BtrI	CACGTC	1 cut(s)	15
BtsCI	GGATG	4 cut(s)	229, 274, 1444, 1477
BtsI	GCAGTG	1 cut(s)	1269
BtsIMutI	CAGTG	6 cut(s)	1269, 1359, 1537, 1565, 1596, 1680
BveI	ACCTGC	1 cut(s)	677
Cac8I	GCNNGC	2 cut(s)	876, 900
CaiI	CAGNNNCTG	1 cut(s)	710
CciI	TCATGA	1 cut(s)	1309
CfoI	GCGC	1 cut(s)	1107
Cfr10I	RCCGGY	1 cut(s)	98
Cfr13I	GGNCC	5 cut(s)	505, 735, 956, 1082, 1364
CseI	GACGC	2 cut(s)	853, 1669
CsiI	ACCWGGT	1 cut(s)	139
Csp6I	GTAC	4 cut(s)	178, 915, 936, 1594
CviAII	CATG	9 cut(s)	392, 560, 675, 803, 1254, 1288, 1310, 1552, 1656
CviQI	GTAC	4 cut(s)	178, 915, 936, 1594
DdeI	CTNAG	3 cut(s)	73, 281, 1329
DpnI	GATC	4 cut(s)	255, 534, 1230, 1537
DpnII	GATC	4 cut(s)	253, 532, 1228, 1535
DraIII	CACNNNGTG	2 cut(s)	143, 1677
EaeI	YGGCCR	1 cut(s)	1527
Ecl136II	GAGCTC	1 cut(s)	1131
Eco24I	GRGCYC	1 cut(s)	1133
Eco47I	GGWCC	5 cut(s)	505, 735, 956, 1082, 1364
Eco53kI	GAGCTC	1 cut(s)	1131
Eco57I	CTGAAG	4 cut(s)	35, 158, 818, 1589
EcoICRI	GAGCTC	1 cut(s)	1131
EcoO109I	RGGNCCY	1 cut(s)	956
EcoRI	GAATTC	1 cut(s)	310
EcoRII	CCWGG	2 cut(s)	139, 1366
EcoT22I	ATGCAT	1 cut(s)	1557
EcoT38I	GRGCYC	1 cut(s)	1133
FaeI	CATG	9 cut(s)	395, 563, 678, 806, 1257, 1291, 1313, 1555, 1659
FalI	AAGNNNNNCTT	2 cut(s)	1440, 1472
FaqI	GGGAC	4 cut(s)	969, 1049, 1209, 1341
FatI	CATG	9 cut(s)	391, 559, 674, 802, 1253, 1287, 1309, 1551, 1655
FauI	CCCGC	1 cut(s)	1186
Fnu4HI	GCNGC	4 cut(s)	497, 1012, 1465, 1653
FokI	GGATG	4 cut(s)	236, 281, 1451, 1484
FriOI	GRGCYC	1 cut(s)	1133
Fsp4HI	GCNGC	4 cut(s)	497, 1012, 1465, 1653
FspBI	CTAG	4 cut(s)	35, 621, 1400, 1404
GlaI	GCGC	1 cut(s)	1106
GluI	GCNGC	4 cut(s)	497, 1012, 1465, 1653
GsuI	CTGGAG	3 cut(s)	600, 732, 739
HaeIII	GGCC	2 cut(s)	98, 1529
HapII	CCGG	2 cut(s)	99, 882
HgaI	GACGC	2 cut(s)	853, 1669
HhaI	GCGC	1 cut(s)	1107
Hin1II	CATG	9 cut(s)	395, 563, 678, 806, 1257, 1291, 1313, 1555, 1659
Hin6I	GCGC	1 cut(s)	1105
HinP1I	GCGC	1 cut(s)	1105
HincII	GTYRAC	1 cut(s)	1099
HindII	GTYRAC	1 cut(s)	1099
HindIII	AAGCTT	1 cut(s)	1454
HinfI	GANTC	6 cut(s)	56, 347, 679, 716, 1354, 1418
HpaII	CCGG	2 cut(s)	99, 882
HphI	GGTGA	2 cut(s)	935, 1529
Hpy166II	GTNNAC	4 cut(s)	407, 767, 1099, 1596
Hpy188I	TCNGA	4 cut(s)	557, 628, 1261, 1349
Hpy188III	TCNNGA	9 cut(s)	53, 262, 536, 579, 711, 756, 1091, 1310, 1541
Hpy8I	GTNNAC	4 cut(s)	407, 767, 1099, 1596
Hpy99I	CGWCG	2 cut(s)	23, 847
HpyAV	CCTTC	5 cut(s)	59, 259, 704, 1564, 1631
HpyCH4III	ACNGT	2 cut(s)	1392, 1675
HpyCH4IV	ACGT	4 cut(s)	14, 403, 1038, 1592
HpyF10VI	GCNNNNNNNGC	2 cut(s)	493, 1661
HpyF3I	CTNAG	3 cut(s)	73, 281, 1329
HpySE526I	ACGT	4 cut(s)	14, 403, 1038, 1592
Hsp92II	CATG	9 cut(s)	395, 563, 678, 806, 1257, 1291, 1313, 1555, 1659
HspAI	GCGC	1 cut(s)	1105
Kzo9I	GATC	4 cut(s)	253, 532, 1228, 1535
LmnI	GCTCC	5 cut(s)	376, 484, 572, 758, 1284
Lsp1109I	GCAGC	4 cut(s)	508, 998, 1476, 1639
LweI	GCATC	3 cut(s)	202, 710, 1418
MabI	ACCWGGT	1 cut(s)	139
MaeI	CTAG	4 cut(s)	35, 621, 1400, 1404
MaeII	ACGT	4 cut(s)	14, 403, 1038, 1592
MaeIII	GTNAC	4 cut(s)	44, 631, 838, 1563
MalI	GATC	4 cut(s)	255, 534, 1230, 1537
MboI	GATC	4 cut(s)	253, 532, 1228, 1535
MboII	GAAGA	5 cut(s)	239, 319, 551, 575, 989
MfeI	CAATTG	1 cut(s)	1026
MflI	RGATCY	3 cut(s)	253, 1228, 1535
MhlI	GDGCHC	4 cut(s)	148, 345, 409, 1133
MlsI	TGGCCA	1 cut(s)	1529
MluNI	TGGCCA	1 cut(s)	1529
MlyI	GAGTC	4 cut(s)	356, 688, 1363, 1412
MmeI	TCCRAC	2 cut(s)	1109, 1256
Mox20I	TGGCCA	1 cut(s)	1529
Mph1103I	ATGCAT	1 cut(s)	1557
MroXI	GAANNNNTTC	1 cut(s)	446
MscI	TGGCCA	1 cut(s)	1529
MseI	TTAA	7 cut(s)	549, 807, 929, 1182, 1212, 1221, 1316
MslI	CAYNNNNRTG	1 cut(s)	654
Msp20I	TGGCCA	1 cut(s)	1529
MspI	CCGG	2 cut(s)	99, 882
MspR9I	CCNGG	3 cut(s)	141, 883, 1368
MunI	CAATTG	1 cut(s)	1026
Mva1269I	GAATGC	1 cut(s)	1324
MvaI	CCWGG	2 cut(s)	141, 1368
MwoI	GCNNNNNNNGC	2 cut(s)	493, 1661
NciI	CCSGG	1 cut(s)	883
NdeII	GATC	4 cut(s)	253, 532, 1228, 1535
NlaIII	CATG	9 cut(s)	395, 563, 678, 806, 1257, 1291, 1313, 1555, 1659
NlaIV	GGNNCC	8 cut(s)	135, 145, 691, 778, 957, 1053, 1230, 1485
NmuCI	GTSAC	3 cut(s)	631, 838, 1563
NsiI	ATGCAT	1 cut(s)	1557
NspI	RCATGY	2 cut(s)	806, 1659
NspV	TTCGAA	1 cut(s)	450
PagI	TCATGA	1 cut(s)	1309
PctI	GAATGC	1 cut(s)	1324
PdmI	GAANNNNTTC	1 cut(s)	446
PfeI	GAWTC	2 cut(s)	56, 716
PkrI	GCNGC	4 cut(s)	498, 1013, 1466, 1654
PleI	GAGTC	4 cut(s)	355, 687, 1362, 1412
PpsI	GAGTC	4 cut(s)	355, 687, 1362, 1412
PpuMI	RGGWCCY	1 cut(s)	956
PshBI	ATTAAT	1 cut(s)	929
Psp124BI	GAGCTC	1 cut(s)	1133
Psp5II	RGGWCCY	1 cut(s)	956
Psp6I	CCWGG	2 cut(s)	139, 1366
PspGI	CCWGG	2 cut(s)	139, 1366
PspN4I	GGNNCC	8 cut(s)	135, 145, 691, 778, 957, 1053, 1230, 1485
PspPI	GGNCC	5 cut(s)	505, 735, 956, 1082, 1364
PspPPI	RGGWCCY	1 cut(s)	956
PstI	CTGCAG	1 cut(s)	1466
PstNI	CAGNNNCTG	1 cut(s)	710
PsuI	RGATCY	3 cut(s)	253, 1228, 1535
RsaI	GTAC	4 cut(s)	179, 916, 937, 1595
RsaNI	GTAC	4 cut(s)	178, 915, 936, 1594
RseI	CAYNNNNRTG	1 cut(s)	654
SacI	GAGCTC	1 cut(s)	1133
SaqAI	TTAA	7 cut(s)	549, 807, 929, 1182, 1212, 1221, 1316
SatI	GCNGC	4 cut(s)	497, 1012, 1465, 1653
Sau3AI	GATC	4 cut(s)	253, 532, 1228, 1535
Sau96I	GGNCC	5 cut(s)	505, 735, 956, 1082, 1364
ScaI	AGTACT	2 cut(s)	179, 937
SchI	GAGTC	4 cut(s)	356, 688, 1363, 1412
ScrFI	CCNGG	3 cut(s)	141, 883, 1368
SduI	GDGCHC	4 cut(s)	148, 345, 409, 1133
SexAI	ACCWGGT	1 cut(s)	139
SfaNI	GCATC	3 cut(s)	202, 710, 1418
SfcI	CTRYAG	1 cut(s)	1462
SfuI	TTCGAA	1 cut(s)	450
SinI	GGWCC	5 cut(s)	505, 735, 956, 1082, 1364
SmiMI	CAYNNNNRTG	1 cut(s)	654
SmlI	CTYRAG	1 cut(s)	199
SmoI	CTYRAG	1 cut(s)	199
SsiI	CCGC	3 cut(s)	508, 1193, 1608
SspMI	CTAG	4 cut(s)	35, 621, 1400, 1404
SstI	GAGCTC	1 cut(s)	1133
StyD4I	CCNGG	3 cut(s)	139, 881, 1366
TaaI	ACNGT	2 cut(s)	1392, 1675
TaiI	ACGT	4 cut(s)	17, 406, 1041, 1595
TaqI	TCGA	3 cut(s)	21, 450, 719
TatI	WGTACW	3 cut(s)	177, 914, 935
TfiI	GAWTC	2 cut(s)	56, 716
Tru1I	TTAA	7 cut(s)	549, 807, 929, 1182, 1212, 1221, 1316
Tru9I	TTAA	7 cut(s)	549, 807, 929, 1182, 1212, 1221, 1316
TscAI	CASTG	6 cut(s)	1276, 1366, 1537, 1572, 1603, 1680
TseFI	GTSAC	3 cut(s)	631, 838, 1563
TseI	GCWGC	4 cut(s)	496, 1011, 1464, 1652
Tsp45I	GTSAC	3 cut(s)	631, 838, 1563
TspDTI	ATGAA	8 cut(s)	240, 576, 837, 1064, 1230, 1242, 1298, 1304
TspGWI	ACGGA	2 cut(s)	7, 411
TspRI	CASTG	6 cut(s)	1276, 1366, 1537, 1572, 1603, 1680
VneI	GTGCAC	1 cut(s)	405
VpaK11BI	GGWCC	5 cut(s)	505, 735, 956, 1082, 1364
VspI	ATTAAT	1 cut(s)	929
XapI	RAATTY	5 cut(s)	28, 127, 310, 906, 1070
XceI	RCATGY	2 cut(s)	806, 1659
XmnI	GAANNNNTTC	1 cut(s)	446
XspI	CTAG	4 cut(s)	35, 621, 1400, 1404
ZrmI	AGTACT	2 cut(s)	179, 937
Zsp2I	ATGCAT	1 cut(s)	1557

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.