MD15G1075300.v1.1

F-box-like WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003410
Physical Location & Seq
Reverse (-)
5129941 .. 5133959
4019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1075300.v1.1.491

Sequence Viewer

Length: 1683 bp
ATGCTTTCCCTCAGTTCCACCGAATTGAACTACCTGGTCTTCCGCTACCTCCACGAATCAGGTTTCACGCACTCAGCTTACGCTTTCGGATTCGAGGCGGGCATCGATAAAAGCTCGATCGACGGAAATGTAGTTCCGCCGGGCGCGCTCGTTACTTTCCTCCAGAAAGGGCTTCAGTTTCTGGAGCTGGAGGCGGACTTGAGCAGCAATAGTGATGCGGAGATGGAGGAAGACTTGTCGTTTCTGCAACCGTTGGATCTCATCACGAAAGATGCATACCAGTTGCAGAAAATGGTGAGGGAGAAGAAGGATAGGATGAACGCCGCGGCGATTGAGAGGAATGTGAGCAAGGAGGAAGGTGGAAGGGAGAATGGGAGGGAAGTAGAGGAGGAGAGGAGAGATAAGGAAAAAGAAGAAGGGGAGGATGGAGACGATAAGCTGGAGAAGGACAAAGAGCAAGAAATTGGTAAAGTTGAGGAGAAGACACACGAGGAGGATTGTAATTTGATGGAGAAGTTGGAACCTGAAGAAAATGAAACTTCTGGGGGGGTGGAACCAATGGATATCACTCCAATCTCAACATCCTTGCCTTGTGAAATTCCAAGTCGCGATTTAACAGTTCTAGAAGGCCATACCTCTGAGGTTTTTGCTTGTGCATGGAGTCCATCAGGTTCCCTTCTTGCATCTGGGTCTGGAGATTCAACGGCGCGAATTTGGACCATTGCAGATGGCAGTTGTGGATCTGGCATGCAAAATGAACCTGCAAATGTTGCAGTGTTGAAGCATTTCAAAGGTAGAGCCAATGAGAAAAGCAAGGACGTGACGACACTTGATTGGAATGGTGATGGGACGTTGCTTGCTACAGGTTCTTATGATGGACAAGCAAGAATATGGAGTAAAGATGGAGAGTTAAAGACTACATTAACCAGACATAAAGGCCCAATCTTTTCTCTGAAATGGAACAAGAAAGGTGATTATCTTTTGAGTGGCAGTGTGGATAAAACAGCAATTGTTTGGGACATAAAGAGCGGAGAATGGAAACAGCAGTTTGAATTTCATTCAGCTCCAACCCTGGATGTTGATTGGCGGAACAATATTTCTTTTGCAACTTGCTCCACCGATACTTTGATACATGTTTGCAAGGTTGGAGAGATCCAACCAATCAAAACTTTCTCAGGGCATCAGGGCGAAGTTAATGCTATTAAGTGGGATCCAACCGGGACCTTGTTGGCTTCTTGCTCTGATGATTATACCGCTAAGATATGGAGTACGAAGCAGGACAAATATTTGCATGATTTGAAGGAACATGTTAAGGAGATATATACTATACGATGGAGTCCTACAGGACCAGGCACAAATAATCCCAATCAGCAGTTAGTGCTAGCAAGTGCTTCCTTTGACTCAACTATAAAACTATGGGATGTGGAACAAGGGAGACTTCTCTACAGCTTCGATGGCCACAGGGATCCGGTATACTCTGTTGCATTCAGCCCAGATGGTGAGTACTTGGCGAGTGGATCAATGGATAAGTGCATGCACATATGGTCCGTGAAGGAAGGCAAAATTGTAAAAACATACACAGGAAATGGTGGGATATTTGAAGTTTGTTGGAACAAGGAAGGCGATAAGGTTGCGGCATGTTTTGCTAACAACATAGTATGTGTCATGGATTTCCGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0001067 GO:0002021 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006508 GO:0006511 GO:0006629 GO:0006807 GO:0006996 GO:0007275 GO:0008013 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009888 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010647 GO:0015630 GO:0016042 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0017053 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0023051 GO:0023056 GO:0030111 GO:0030154 GO:0030163 GO:0030177 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031667 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0035264 GO:0035601 GO:0036211 GO:0040007 GO:0042393 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044212 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0045444 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0047485 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050872 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051603 GO:0060255 GO:0060612 GO:0060613 GO:0060828 GO:0061448 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090207 GO:0090263 GO:0097159 GO:0098732 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140110 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04310 map04013 map04310
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

561

Amino Acids

62.31

Weight (kDa)

4.86

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 8 - 32 1.8e-06 LisH
Beta-prop_WDR5 PF25175 205 - 252 2.3e-09 WDR5 beta-propeller domain
WD40 PF00400 205 - 239 1.9e-10 WD domain, G-beta repeat
Beta-prop_EML PF23409 206 - 343 4.1e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 206 - 251 4.7e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 206 - 245 5.9e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 207 - 249 2.7e-07 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 207 - 312 6.1e-08 CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 265 - 516 7.2e-13 MABP1/WDR62 second WD40 domain
Beta-prop_IFT140_1st PF23383 266 - 342 6.2e-06 IFT140 first beta-propeller
Beta-prop_EML_2 PF23414 266 - 362 5.1e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 268 - 379 1.9e-20 WDR5 beta-propeller domain
WD40_Prp19 PF24814 268 - 347 4.8e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 268 - 382 1e-18 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 270 - 516 5.5e-11 WDR36/Utp21 first beta-propeller
Beta-prop_Aladin PF25460 270 - 343 7.3e-07 Aladin seven-bladed propeller
WD40 PF00400 271 - 299 3.9e-07 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 271 - 353 1.1e-12 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 271 - 538 1.9e-53 THOC3 beta-propeller domain
EIF3I PF24805 271 - 374 1.4e-10 EIF3I
WDR55 PF24796 272 - 431 1.1e-10 WDR55
Beta-prop_WDR3_2nd PF25172 274 - 379 3.5e-08 WDR3 second beta-propeller domain
WD40 PF00400 303 - 340 8.1e-08 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 306 - 449 2.3e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 307 - 382 1.2e-08 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 308 - 484 4.5e-27 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 317 - 547 9.7e-36 Prp19 WD40 domain
WD40_Gbeta PF25391 326 - 430 1.6e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 365 - 474 4.2e-18 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 367 - 558 8.7e-42 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 367 - 548 5.6e-27 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 367 - 494 7.1e-09 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 379 - 544 4.2e-14 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 379 - 559 4.2e-08 MABP1/WDR62 second WD40 domain
WD40 PF00400 386 - 423 5.1e-11 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 388 - 524 8.8e-16 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 391 - 525 7.8e-07 Echinoderm microtubule-associated protein first beta-propeller
WDR90_beta-prop_4th PF23342 398 - 524 3.8e-09 WDR90, 4th beta-propeller
Beta-prop_WDR41 PF25178 410 - 534 1.5e-06 WDR41
Beta-prop_CAF1B_HIR1 PF24105 433 - 525 3.5e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 454 - 557 9.1e-09 Echinoderm microtubule-associated protein second beta-propeller
NBCH_WD40 PF20426 456 - 524 1e-06 Neurobeachin beta propeller domain
Beta-prop_IFT122_1st PF23381 457 - 550 1.2e-12 IFT122 first beta-propeller
WD40_CDC20-Fz PF24807 457 - 548 1.4e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 457 - 546 1e-16 WDR3 first beta-propeller domain
WDR55 PF24796 458 - 527 1e-06 WDR55
Beta-prop_TEP1_2nd PF25047 459 - 520 1e-07 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 460 - 552 4.6e-10 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 460 - 549 1.7e-10 EIF3I
Beta-prop_WDR90_POC16_2nd PF23393 460 - 546 6.1e-08 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 463 - 559 7.7e-12 G protein beta WD-40 repeat protein
WD40 PF00400 478 - 516 3.1e-12 WD domain, G-beta repeat
Beta-prop_Aladin PF25460 489 - 558 1.5e-07 Aladin seven-bladed propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 769
AccBSI CCGCTC 1 cut(s) 1029
AccI GTMKAC 1 cut(s) 1473
AccII CGCG 4 cut(s) 146, 326, 609, 709
AclWI GGATC 8 cut(s) 264, 748, 1147, 1205, 1218, 1460, 1473, 1525
AcoI YGGCCR 1 cut(s) 1456
AcsI RAATTY 3 cut(s) 597, 711, 1052
AcuI CTGAAG 2 cut(s) 158, 546
AfaI GTAC 2 cut(s) 1270, 1505
AflIII ACRYGT 2 cut(s) 1132, 1306
AgsI TTSAA 7 cut(s) 28, 702, 781, 790, 1052, 1300, 1601
AjiI CACGTC 1 cut(s) 820
AjnI CCWGG 3 cut(s) 33, 1071, 1348
AloI GAACNNNNNNTCC 2 cut(s) 117, 149
AluBI AGCT 6 cut(s) 77, 114, 187, 439, 1064, 1449
AluI AGCT 6 cut(s) 77, 114, 187, 439, 1064, 1449
Alw26I GTCTC 2 cut(s) 423, 1429
AlwI GGATC 8 cut(s) 264, 748, 1147, 1205, 1218, 1460, 1473, 1525
AlwNI CAGNNNCTG 1 cut(s) 181
AoxI GGCC 3 cut(s) 628, 937, 1456
ApeKI GCWGC 1 cut(s) 204
ApoI RAATTY 3 cut(s) 597, 711, 1052
Asp700I GAANNNNTTC 1 cut(s) 785
AspLEI GCGC 3 cut(s) 146, 148, 709
AspS9I GGNCC 5 cut(s) 717, 938, 1221, 1346, 1545
AsuC2I CCSGG 2 cut(s) 141, 1219
AsuHPI GGTGA 4 cut(s) 307, 854, 983, 1511
AsuNHI GCTAGC 1 cut(s) 1381
AvaII GGWCC 4 cut(s) 717, 1221, 1346, 1545
BalI TGGCCA 1 cut(s) 1458
BamHI GGATCC 2 cut(s) 1210, 1465
BauI CACGAG 1 cut(s) 488
BbsI GAAGAC 3 cut(s) 31, 237, 488
BbvI GCAGC 1 cut(s) 216
BceAI ACGGC 1 cut(s) 720
BcgI CGANNNNNNTGC 4 cut(s) 1178, 1212, 1613, 1647
BciT130I CCWGG 3 cut(s) 35, 1073, 1350
BcnI CCSGG 2 cut(s) 141, 1219
BcoDI GTCTC 2 cut(s) 423, 1429
BfaI CTAG 2 cut(s) 623, 1382
BfmI CTRYAG 3 cut(s) 861, 1341, 1444
BfuAI ACCTGC 1 cut(s) 769
BisI GCNGC 4 cut(s) 205, 324, 327, 1635
BlsI GCNGC 4 cut(s) 206, 325, 328, 1636
BmcAI AGTACT 1 cut(s) 1505
Bme1390I CCNGG 5 cut(s) 35, 141, 1073, 1219, 1350
Bme18I GGWCC 4 cut(s) 717, 1221, 1346, 1545
BmgBI CACGTC 1 cut(s) 820
BmgT120I GGNCC 5 cut(s) 717, 938, 1221, 1346, 1545
BmiI GGNNCC 6 cut(s) 522, 555, 673, 1212, 1222, 1467
BmrFI CCNGG 5 cut(s) 35, 141, 1073, 1219, 1350
BmsI GCATC 5 cut(s) 111, 205, 262, 692, 1189
BmtI GCTAGC 1 cut(s) 1385
BpiI GAAGAC 3 cut(s) 31, 237, 488
BpmI CTGGAG 5 cut(s) 146, 203, 209, 461, 714
BpuEI CTTGAG 1 cut(s) 220
BpuMI CCSGG 2 cut(s) 141, 1219
Bsa29I ATCGAT 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 324, 1071
BsaWI WCCGGW 1 cut(s) 1468
Bse1I ACTGG 1 cut(s) 280
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 3 cut(s) 35, 1073, 1350
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 324, 1071
BseGI GGATG 5 cut(s) 321, 430, 581, 1081, 1426
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 4 cut(s) 25, 87, 630, 1188
BseNI ACTGG 1 cut(s) 280
BsePI GCGCGC 1 cut(s) 144
BseRI GAGGAG 5 cut(s) 401, 404, 409, 491, 506
BseXI GCAGC 1 cut(s) 216
Bsh1236I CGCG 4 cut(s) 146, 326, 609, 709
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 630, 939, 1458
BshVI ATCGAT 1 cut(s) 105
BsiEI CGRYCG 1 cut(s) 120
BsiSI CCGG 3 cut(s) 140, 1218, 1469
BslFI GGGAC 3 cut(s) 862, 1031, 1234
BsmAI GTCTC 2 cut(s) 423, 1429
BsmBI CGTCTC 1 cut(s) 423
BsmFI GGGAC 3 cut(s) 862, 1031, 1234
BsmI GAATGC 1 cut(s) 1484
BsnI GGCC 3 cut(s) 630, 939, 1458
Bsp143I GATC 7 cut(s) 117, 256, 740, 1152, 1210, 1465, 1517
Bsp68I TCGCGA 1 cut(s) 609
BspANI GGCC 3 cut(s) 630, 939, 1458
BspCNI CTCAG 4 cut(s) 24, 86, 631, 1187
BspDI ATCGAT 1 cut(s) 105
BspFNI CGCG 4 cut(s) 146, 326, 609, 709
BspLI GGNNCC 6 cut(s) 522, 555, 673, 1212, 1222, 1467
BspMI ACCTGC 1 cut(s) 769
BspOI GCTAGC 1 cut(s) 1385
BspPI GGATC 8 cut(s) 264, 748, 1147, 1205, 1218, 1460, 1473, 1525
BsrBI CCGCTC 1 cut(s) 1029
BsrDI GCAATG 1 cut(s) 720
BsrI ACTGG 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 324, 1071
BssHII GCGCGC 1 cut(s) 144
BssMI GATC 7 cut(s) 117, 256, 740, 1152, 1210, 1465, 1517
BssNAI GTATAC 1 cut(s) 1474
BssSI CACGAG 1 cut(s) 488
Bst1107I GTATAC 1 cut(s) 1474
Bst2BI CACGAG 1 cut(s) 488
Bst2UI CCWGG 3 cut(s) 35, 1073, 1350
Bst4CI ACNGT 2 cut(s) 252, 619
BstAPI GCANNNNNTGC 3 cut(s) 770, 1378, 1643
BstC8I GCNNGC 6 cut(s) 100, 146, 749, 858, 1383, 1535
BstDEI CTNAG 5 cut(s) 11, 73, 639, 1174, 1257
BstDSI CCRYGG 1 cut(s) 324
BstF5I GGATG 5 cut(s) 321, 430, 581, 1081, 1426
BstFNI CGCG 4 cut(s) 146, 326, 609, 709
BstHHI GCGC 3 cut(s) 146, 148, 709
BstKTI GATC 7 cut(s) 120, 259, 743, 1155, 1213, 1468, 1520
BstMAI GTCTC 2 cut(s) 423, 1429
BstMBI GATC 7 cut(s) 117, 256, 740, 1152, 1210, 1465, 1517
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 5 cut(s) 145, 770, 1378, 1455, 1643
BstNI CCWGG 3 cut(s) 35, 1073, 1350
BstNSI RCATGY 5 cut(s) 751, 1136, 1310, 1537, 1641
BstSCI CCNGG 5 cut(s) 33, 139, 1071, 1217, 1348
BstSFI CTRYAG 3 cut(s) 861, 1341, 1444
BstUI CGCG 4 cut(s) 146, 326, 609, 709
BstV1I GCAGC 1 cut(s) 216
BstV2I GAAGAC 3 cut(s) 31, 237, 488
BstX2I RGATCY 5 cut(s) 256, 740, 1152, 1210, 1465
BstYI RGATCY 5 cut(s) 256, 740, 1152, 1210, 1465
BstZ17I GTATAC 1 cut(s) 1474
Bsu15I ATCGAT 1 cut(s) 105
BsuRI GGCC 3 cut(s) 630, 939, 1458
BsuTUI ATCGAT 1 cut(s) 105
BtgI CCRYGG 1 cut(s) 324
BtrI CACGTC 1 cut(s) 820
BtsCI GGATG 5 cut(s) 321, 430, 581, 1081, 1426
BtsI GCAGTG 2 cut(s) 780, 997
BtsIMutI CAGTG 2 cut(s) 780, 997
BtuMI TCGCGA 1 cut(s) 609
BveI ACCTGC 1 cut(s) 769
Cac8I GCNNGC 6 cut(s) 100, 146, 749, 858, 1383, 1535
CaiI CAGNNNCTG 1 cut(s) 181
CfoI GCGC 3 cut(s) 146, 148, 709
Cfr13I GGNCC 5 cut(s) 717, 938, 1221, 1346, 1545
Cfr42I CCGCGG 1 cut(s) 327
ClaI ATCGAT 1 cut(s) 105
CsiI ACCWGGT 1 cut(s) 33
Csp6I GTAC 2 cut(s) 1269, 1504
CviAII CATG 8 cut(s) 657, 748, 1133, 1292, 1307, 1534, 1638, 1666
CviQI GTAC 2 cut(s) 1269, 1504
DdeI CTNAG 5 cut(s) 11, 73, 639, 1174, 1257
DpnI GATC 7 cut(s) 119, 258, 742, 1154, 1212, 1467, 1519
DpnII GATC 7 cut(s) 117, 256, 740, 1152, 1210, 1465, 1517
EaeI YGGCCR 1 cut(s) 1456
EciI GGCGGA 3 cut(s) 126, 209, 1102
Eco32I GATATC 1 cut(s) 565
Eco47I GGWCC 4 cut(s) 717, 1221, 1346, 1545
Eco57I CTGAAG 2 cut(s) 158, 546
EcoO109I RGGNCCY 1 cut(s) 1221
EcoRII CCWGG 3 cut(s) 33, 1071, 1348
EcoRV GATATC 1 cut(s) 565
EcoT22I ATGCAT 1 cut(s) 277
Esp3I CGTCTC 1 cut(s) 423
FaeI CATG 8 cut(s) 660, 751, 1136, 1295, 1310, 1537, 1641, 1669
FalI AAGNNNNNCTT 2 cut(s) 1422, 1454
FaqI GGGAC 3 cut(s) 862, 1031, 1234
FatI CATG 8 cut(s) 656, 747, 1132, 1291, 1306, 1533, 1637, 1665
FauI CCCGC 1 cut(s) 91
FauNDI CATATG 1 cut(s) 1541
FblI GTMKAC 1 cut(s) 1473
Fnu4HI GCNGC 4 cut(s) 205, 324, 327, 1635
FokI GGATG 5 cut(s) 328, 437, 568, 1088, 1433
Fsp4HI GCNGC 4 cut(s) 205, 324, 327, 1635
FspBI CTAG 2 cut(s) 623, 1382
GlaI GCGC 3 cut(s) 145, 147, 708
GluI GCNGC 4 cut(s) 205, 324, 327, 1635
GsuI CTGGAG 5 cut(s) 146, 203, 209, 461, 714
HaeIII GGCC 3 cut(s) 630, 939, 1458
HapII CCGG 3 cut(s) 140, 1218, 1469
HhaI GCGC 3 cut(s) 146, 148, 709
Hin1II CATG 8 cut(s) 660, 751, 1136, 1295, 1310, 1537, 1641, 1669
Hin6I GCGC 3 cut(s) 144, 146, 707
HinP1I GCGC 3 cut(s) 144, 146, 707
HinfI GANTC 6 cut(s) 56, 90, 661, 698, 1336, 1400
HpaII CCGG 3 cut(s) 140, 1218, 1469
HphI GGTGA 4 cut(s) 307, 854, 983, 1511
Hpy166II GTNNAC 1 cut(s) 1474
Hpy188I TCNGA 5 cut(s) 89, 640, 954, 1243, 1676
Hpy188III TCNNGA 6 cut(s) 163, 182, 265, 608, 623, 693
Hpy8I GTNNAC 1 cut(s) 1474
Hpy99I CGWCG 1 cut(s) 125
HpyCH4III ACNGT 2 cut(s) 252, 619
HpyCH4IV ACGT 2 cut(s) 819, 851
HpyF10VI GCNNNNNNNGC 5 cut(s) 145, 770, 1378, 1455, 1643
HpyF3I CTNAG 5 cut(s) 11, 73, 639, 1174, 1257
HpySE526I ACGT 2 cut(s) 819, 851
Hsp92II CATG 8 cut(s) 660, 751, 1136, 1295, 1310, 1537, 1641, 1669
HspAI GCGC 3 cut(s) 144, 146, 707
KspI CCGCGG 1 cut(s) 327
Kzo9I GATC 7 cut(s) 117, 256, 740, 1152, 1210, 1465, 1517
LmnI GCTCC 3 cut(s) 184, 1069, 1118
Lsp1109I GCAGC 1 cut(s) 216
LweI GCATC 5 cut(s) 111, 205, 262, 692, 1189
MabI ACCWGGT 1 cut(s) 33
MaeI CTAG 2 cut(s) 623, 1382
MaeII ACGT 2 cut(s) 819, 851
MaeIII GTNAC 2 cut(s) 151, 820
MalI GATC 7 cut(s) 119, 258, 742, 1154, 1212, 1467, 1519
MbiI CCGCTC 1 cut(s) 1029
MboI GATC 7 cut(s) 117, 256, 740, 1152, 1210, 1465, 1517
MboII GAAGA 6 cut(s) 31, 242, 316, 425, 493, 539
MfeI CAATTG 1 cut(s) 1008
MflI RGATCY 5 cut(s) 256, 740, 1152, 1210, 1465
MlsI TGGCCA 1 cut(s) 1458
MluCI AATT 8 cut(s) 23, 462, 502, 597, 711, 1008, 1052, 1563
MluNI TGGCCA 1 cut(s) 1458
MlyI GAGTC 3 cut(s) 670, 1345, 1394
MmeI TCCRAC 7 cut(s) 234, 498, 1091, 1126, 1180, 1238, 1589
Mox20I TGGCCA 1 cut(s) 1458
Mph1103I ATGCAT 1 cut(s) 277
MroXI GAANNNNTTC 1 cut(s) 785
MscI TGGCCA 1 cut(s) 1458
MseI TTAA 6 cut(s) 614, 911, 923, 1194, 1203, 1311
Msp20I TGGCCA 1 cut(s) 1458
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 3 cut(s) 140, 1218, 1469
MspR9I CCNGG 5 cut(s) 35, 141, 1073, 1219, 1350
MunI CAATTG 1 cut(s) 1008
Mva1269I GAATGC 1 cut(s) 1484
MvaI CCWGG 3 cut(s) 35, 1073, 1350
MvnI CGCG 4 cut(s) 146, 326, 609, 709
MwoI GCNNNNNNNGC 5 cut(s) 145, 770, 1378, 1455, 1643
NciI CCSGG 2 cut(s) 141, 1219
NdeI CATATG 1 cut(s) 1541
NdeII GATC 7 cut(s) 117, 256, 740, 1152, 1210, 1465, 1517
NheI GCTAGC 1 cut(s) 1381
NlaIII CATG 8 cut(s) 660, 751, 1136, 1295, 1310, 1537, 1641, 1669
NlaIV GGNNCC 6 cut(s) 522, 555, 673, 1212, 1222, 1467
NmuCI GTSAC 1 cut(s) 820
NruI TCGCGA 1 cut(s) 609
NsiI ATGCAT 1 cut(s) 277
NspI RCATGY 5 cut(s) 751, 1136, 1310, 1537, 1641
PaeI GCATGC 2 cut(s) 751, 1537
PauI GCGCGC 1 cut(s) 144
PciI ACATGT 2 cut(s) 1132, 1306
PctI GAATGC 1 cut(s) 1484
PdmI GAANNNNTTC 1 cut(s) 785
PfeI GAWTC 3 cut(s) 56, 90, 698
PkrI GCNGC 4 cut(s) 206, 325, 328, 1636
Ple19I CGATCG 1 cut(s) 120
PleI GAGTC 3 cut(s) 669, 1344, 1394
PpsI GAGTC 3 cut(s) 669, 1344, 1394
PpuMI RGGWCCY 1 cut(s) 1221
PscI ACATGT 2 cut(s) 1132, 1306
Psp5II RGGWCCY 1 cut(s) 1221
Psp6I CCWGG 3 cut(s) 33, 1071, 1348
PspGI CCWGG 3 cut(s) 33, 1071, 1348
PspN4I GGNNCC 6 cut(s) 522, 555, 673, 1212, 1222, 1467
PspPI GGNCC 5 cut(s) 717, 938, 1221, 1346, 1545
PspPPI RGGWCCY 1 cut(s) 1221
PstNI CAGNNNCTG 1 cut(s) 181
PsuI RGATCY 5 cut(s) 256, 740, 1152, 1210, 1465
PteI GCGCGC 1 cut(s) 144
PvuI CGATCG 1 cut(s) 120
RruI TCGCGA 1 cut(s) 609
RsaI GTAC 2 cut(s) 1270, 1505
RsaNI GTAC 2 cut(s) 1269, 1504
SacII CCGCGG 1 cut(s) 327
SaqAI TTAA 6 cut(s) 614, 911, 923, 1194, 1203, 1311
SatI GCNGC 4 cut(s) 205, 324, 327, 1635
Sau3AI GATC 7 cut(s) 117, 256, 740, 1152, 1210, 1465, 1517
Sau96I GGNCC 5 cut(s) 717, 938, 1221, 1346, 1545
ScaI AGTACT 1 cut(s) 1505
SchI GAGTC 3 cut(s) 670, 1345, 1394
ScrFI CCNGG 5 cut(s) 35, 141, 1073, 1219, 1350
SexAI ACCWGGT 1 cut(s) 33
SfaNI GCATC 5 cut(s) 111, 205, 262, 692, 1189
SfcI CTRYAG 3 cut(s) 861, 1341, 1444
Sfr303I CCGCGG 1 cut(s) 327
SgrBI CCGCGG 1 cut(s) 327
SinI GGWCC 4 cut(s) 717, 1221, 1346, 1545
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
SphI GCATGC 2 cut(s) 751, 1537
Sse9I AATT 8 cut(s) 23, 462, 502, 597, 711, 1008, 1052, 1563
SspI AATATT 2 cut(s) 1096, 1286
SspMI CTAG 2 cut(s) 623, 1382
StyD4I CCNGG 5 cut(s) 33, 139, 1071, 1217, 1348
TaaI ACNGT 2 cut(s) 252, 619
TaiI ACGT 2 cut(s) 822, 854
TaqI TCGA 5 cut(s) 93, 105, 116, 120, 1452
TasI AATT 8 cut(s) 23, 462, 502, 597, 711, 1008, 1052, 1563
TatI WGTACW 1 cut(s) 1503
TauI GCSGC 3 cut(s) 326, 329, 1637
TfiI GAWTC 3 cut(s) 56, 90, 698
Tru1I TTAA 6 cut(s) 614, 911, 923, 1194, 1203, 1311
Tru9I TTAA 6 cut(s) 614, 911, 923, 1194, 1203, 1311
TscAI CASTG 2 cut(s) 780, 997
TseFI GTSAC 1 cut(s) 820
TseI GCWGC 1 cut(s) 204
Tsp45I GTSAC 1 cut(s) 820
TspDTI ATGAA 4 cut(s) 332, 549, 771, 1046
TspGWI ACGGA 2 cut(s) 138, 1537
TspRI CASTG 2 cut(s) 780, 997
VpaK11BI GGWCC 4 cut(s) 717, 1221, 1346, 1545
XapI RAATTY 3 cut(s) 597, 711, 1052
XbaI TCTAGA 1 cut(s) 622
XceI RCATGY 5 cut(s) 751, 1136, 1310, 1537, 1641
XmiI GTMKAC 1 cut(s) 1473
XmnI GAANNNNTTC 1 cut(s) 785
XspI CTAG 2 cut(s) 623, 1382
ZrmI AGTACT 1 cut(s) 1505
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.