Rh2CG195500

F-box-like WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003410
Physical Location & Seq
Forward (+)
18654207 .. 18663765
9559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG195500.1

Sequence Viewer

Length: 1242 bp
ATGACCTCCATCACCTCCGTCGAGCTCAATTTCCTCGTCTTCCGTTATCTTCAGGAATCAGGCTTCACACACTCGGCTTTTGCTCTAGGATATGAGGCCGGTATTAACAAATGCACTATAGATGGAAATTTGATTCCACCTGGTGCTCTCATTACATTTGTTCAAAAAGGGCTTCAGTACTTGGAGATGGAAGCCAACTTGAGTAATAGTGATGCAGAGGTTGATGAGGATTTCTCTCTTTTACAACCTTTAGATCTCATCCAAAATGATGTTAATCAACTACGCCAATTGATAAAAGATAGAAAGAAAAATTTGCAGAAGGATAGAGATAAGGACAAAGACCTTGAGAAAGAACATGAAGGAGAGCGTACACGAGTAAGAGAGAAGGAAAGACTAGAAAGGGAGAAGGAATTTGAAAAGAGCAGAGAGAGGGTAGAAAAGAATAAGGAGCTAGAAAAGAATAAGGAGGTAGAAAAGAACAAGGAGCAAGAAAAGCATCATGAGGACCATAACGACAGAGGTAGGGCTACAGAACAGGAAGAAAAAGTCAATGTCAAACATGAAGAAAATGGAGCTTCTGCAGAACCAATGGATATCTCGACAGCTTCAACATCTCAGGCATGTGAAATCCCTAGTTCTGATGTGACCATTCTAGAAGGCCATACATCTGAGGTCTGTGCTTGTGCTTGGAGTCCAACTGGATCTCTTCTTGCATCTGGGTCTGGAGATTCTACAGCTCGCATTTGGCCAATTCCAGATGGAAGCTCTAGATCTGGTTTACAAAATGGTGCATTAAATGTGTTGGTGCTAAAGCATGTTAAGGGTAGAACAAATGAAAAGAGTAAAGATGTCACGACTCTTGATTGGAATGGAGAGGGAACACTGCTTGCAACTGGCTCATATGATGGGCAGGCAAGAATTTGGAGTACAAATGGTGAACTAAGGAGTACTTTGAACAAACATAAAGGTCCCATATTCTCTCTAAAGTGGAACAAGAAGGGAGATTATCTGCTTACGGGAAGTTGTGATAAAACTGCAATTGTGTGGGATGTGAAGGCAGAGGAAGTGAAGCAACAATTTGAGTTTCATTCAGGTCCAACACTTGATGTTGACTGGCGCAACAATATGCAATTTGCAATGAGCTCCACAGACAATATGATATATGTTTGCAAGATTGGGGAAAACCGCCCTATTAAAACTTTTGCAGGACATCAGGTAAGCTTATACATTCAGCCTCTTTAG

Protein Analysis

413

Amino Acids

46.57

Weight (kDa)

5.47

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 8 - 33 6.6e-08 LisH
WD40_CDC20-Fz PF24807 213 - 252 7e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 214 - 256 1.5e-07 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 214 - 323 1.4e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 215 - 249 1.5e-10 WD domain, G-beta repeat
Beta-prop_EML PF23409 218 - 388 1.9e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 221 - 253 2.2e-06 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 272 - 353 3e-06 IFT140 first beta-propeller
WD40_Prp19 PF24814 274 - 339 2.9e-06 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 277 - 406 7.8e-21 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 277 - 406 6.9e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 278 - 402 9e-20 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 278 - 405 6.8e-18 CDC20/Fizzy WD40 domain
Beta-prop_WDR19_1st PF23389 280 - 376 4e-06 WDR19 first beta-propeller
WD40 PF00400 281 - 309 2.8e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 281 - 387 2.5e-09 Echinoderm microtubule-associated protein second beta-propeller
EIF3I PF24805 281 - 387 2.6e-12 EIF3I
WD40_WDHD1_1st PF24817 281 - 362 1.3e-11 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 281 - 400 6.1e-18 THOC3 beta-propeller domain
WDR55 PF24796 282 - 387 2.2e-06 WDR55
Beta-prop_WDR3_2nd PF25172 284 - 389 9.3e-08 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 295 - 407 2.6e-06 TEP-1 second beta-propeller
WD40 PF00400 313 - 350 7.8e-08 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 317 - 399 8.6e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 321 - 388 5.3e-06 Aladin seven-bladed propeller
WD40_Prp19 PF24814 327 - 405 3.3e-07 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 1186
AclWI GGATC 1 cut(s) 709
AcoI YGGCCR 1 cut(s) 746
AcsI RAATTY 4 cut(s) 127, 310, 410, 918
AcuI CTGAAG 2 cut(s) 35, 158
AdeI CACNNNGTG 1 cut(s) 143
AfaI GTAC 4 cut(s) 179, 370, 928, 949
AgsI TTSAA 4 cut(s) 164, 416, 609, 955
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 8 cut(s) 25, 451, 575, 605, 737, 765, 1143, 1221
AluI AGCT 8 cut(s) 25, 451, 575, 605, 737, 765, 1143, 1221
Alw21I GWGCWC 3 cut(s) 27, 148, 1145
AlwI GGATC 1 cut(s) 709
AoxI GGCC 3 cut(s) 96, 658, 746
ApoI RAATTY 4 cut(s) 127, 310, 410, 918
AspLEI GCGC 1 cut(s) 1119
AspS9I GGNCC 3 cut(s) 505, 968, 1094
AsuHPI GGTGA 2 cut(s) 4, 947
AvaII GGWCC 3 cut(s) 505, 968, 1094
BalI TGGCCA 1 cut(s) 748
BanII GRGCYC 2 cut(s) 27, 1145
BauI CACGAG 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 3 cut(s) 27, 148, 1145
BccI CCATC 5 cut(s) 17, 116, 181, 752, 899
BciT130I CCWGG 1 cut(s) 141
BfaI CTAG 6 cut(s) 86, 395, 452, 633, 653, 768
BfmI CTRYAG 4 cut(s) 117, 528, 579, 732
BglII AGATCT 2 cut(s) 253, 770
BmcAI AGTACT 2 cut(s) 179, 949
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 3 cut(s) 505, 968, 1094
BmgT120I GGNCC 3 cut(s) 505, 968, 1094
BmiI GGNNCC 1 cut(s) 970
BmrFI CCNGG 1 cut(s) 141
BmsI GCATC 3 cut(s) 202, 505, 722
BpiI GAAGAC 1 cut(s) 31
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpmI CTGGAG 1 cut(s) 744
BpuEI CTTGAG 2 cut(s) 220, 365
BsaBI GATNNNNATC 1 cut(s) 273
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 3 cut(s) 703, 898, 1118
Bse3DI GCAATG 1 cut(s) 1143
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 1 cut(s) 141
BseGI GGATG 2 cut(s) 258, 1054
BseJI GATNNNNATC 1 cut(s) 273
BseMI GCAATG 1 cut(s) 1143
BseMII CTCAG 2 cut(s) 629, 660
BseNI ACTGG 3 cut(s) 703, 898, 1118
BshFI GGCC 3 cut(s) 98, 660, 748
BsiHKAI GWGCWC 3 cut(s) 27, 148, 1145
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 954
BsmFI GGGAC 1 cut(s) 954
BsnI GGCC 3 cut(s) 98, 660, 748
Bsp1286I GDGCHC 3 cut(s) 27, 148, 1145
Bsp143I GATC 3 cut(s) 253, 701, 770
BspACI CCGC 1 cut(s) 1186
BspANI GGCC 3 cut(s) 98, 660, 748
BspCNI CTCAG 2 cut(s) 628, 661
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 1 cut(s) 970
BspMAI CTGCAG 1 cut(s) 583
BspPI GGATC 1 cut(s) 709
BsrDI GCAATG 1 cut(s) 1143
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 3 cut(s) 703, 898, 1118
BssAI RCCGGY 1 cut(s) 98
BssMI GATC 3 cut(s) 253, 701, 770
BssSI CACGAG 1 cut(s) 372
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 141
Bst6I CTCTTC 1 cut(s) 711
BstC8I GCNNGC 3 cut(s) 739, 888, 912
BstDEI CTNAG 3 cut(s) 615, 669, 941
BstF5I GGATG 2 cut(s) 258, 1054
BstHHI GCGC 1 cut(s) 1119
BstKTI GATC 3 cut(s) 256, 704, 773
BstMBI GATC 3 cut(s) 253, 701, 770
BstMWI GCNNNNNNNGC 1 cut(s) 493
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 2 cut(s) 624, 818
BstSCI CCNGG 1 cut(s) 139
BstSFI CTRYAG 4 cut(s) 117, 528, 579, 732
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 3 cut(s) 253, 701, 770
BstYI RGATCY 3 cut(s) 253, 701, 770
BsuRI GGCC 3 cut(s) 98, 660, 748
BtsCI GGATG 2 cut(s) 258, 1054
BtsI GCAGTG 1 cut(s) 881
BtsIMutI CAGTG 1 cut(s) 881
Cac8I GCNNGC 3 cut(s) 739, 888, 912
CciI TCATGA 1 cut(s) 499
CfoI GCGC 1 cut(s) 1119
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 505, 968, 1094
CsiI ACCWGGT 1 cut(s) 139
Csp6I GTAC 4 cut(s) 178, 369, 927, 948
CviAII CATG 5 cut(s) 356, 500, 560, 621, 815
CviQI GTAC 4 cut(s) 178, 369, 927, 948
DdeI CTNAG 3 cut(s) 615, 669, 941
DpnI GATC 3 cut(s) 255, 703, 772
DpnII GATC 3 cut(s) 253, 701, 770
DraIII CACNNNGTG 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 746
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
Ecl136II GAGCTC 2 cut(s) 25, 1143
Eco24I GRGCYC 2 cut(s) 27, 1145
Eco32I GATATC 1 cut(s) 595
Eco47I GGWCC 3 cut(s) 505, 968, 1094
Eco53kI GAGCTC 2 cut(s) 25, 1143
Eco57I CTGAAG 2 cut(s) 35, 158
EcoICRI GAGCTC 2 cut(s) 25, 1143
EcoO109I RGGNCCY 1 cut(s) 968
EcoRII CCWGG 1 cut(s) 139
EcoRV GATATC 1 cut(s) 595
EcoT38I GRGCYC 2 cut(s) 27, 1145
FaeI CATG 5 cut(s) 359, 503, 563, 624, 818
FalI AAGNNNNNCTT 2 cut(s) 934, 966
FaqI GGGAC 1 cut(s) 954
FatI CATG 5 cut(s) 355, 499, 559, 620, 814
FauNDI CATATG 1 cut(s) 901
FokI GGATG 2 cut(s) 245, 1061
FriOI GRGCYC 2 cut(s) 27, 1145
FspBI CTAG 6 cut(s) 86, 395, 452, 633, 653, 768
GlaI GCGC 1 cut(s) 1118
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 3 cut(s) 98, 660, 748
HapII CCGG 1 cut(s) 99
HhaI GCGC 1 cut(s) 1119
Hin1II CATG 5 cut(s) 359, 503, 563, 624, 818
Hin6I GCGC 1 cut(s) 1117
HinP1I GCGC 1 cut(s) 1117
HincII GTYRAC 1 cut(s) 1111
HindII GTYRAC 1 cut(s) 1111
HindIII AAGCTT 1 cut(s) 1219
HinfI GANTC 5 cut(s) 56, 133, 691, 728, 856
HpaII CCGG 1 cut(s) 99
HphI GGTGA 2 cut(s) 4, 947
Hpy166II GTNNAC 4 cut(s) 371, 779, 938, 1111
Hpy188I TCNGA 2 cut(s) 640, 670
Hpy188III TCNNGA 9 cut(s) 53, 500, 598, 653, 723, 755, 768, 853, 860
Hpy8I GTNNAC 4 cut(s) 371, 779, 938, 1111
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 7 cut(s) 313, 353, 379, 400, 650, 991, 1048
HpyF10VI GCNNNNNNNGC 1 cut(s) 493
HpyF3I CTNAG 3 cut(s) 615, 669, 941
Hsp92II CATG 5 cut(s) 359, 503, 563, 624, 818
HspAI GCGC 1 cut(s) 1117
Kzo9I GATC 3 cut(s) 253, 701, 770
LmnI GCTCC 4 cut(s) 448, 484, 572, 1148
LweI GCATC 3 cut(s) 202, 505, 722
MabI ACCWGGT 1 cut(s) 139
MaeI CTAG 6 cut(s) 86, 395, 452, 633, 653, 768
MaeIII GTNAC 2 cut(s) 643, 850
MalI GATC 3 cut(s) 255, 703, 772
MboI GATC 3 cut(s) 253, 701, 770
MboII GAAGA 5 cut(s) 31, 41, 551, 575, 698
MfeI CAATTG 2 cut(s) 287, 1038
MflI RGATCY 3 cut(s) 253, 701, 770
MhlI GDGCHC 3 cut(s) 27, 148, 1145
MlsI TGGCCA 1 cut(s) 748
MluNI TGGCCA 1 cut(s) 748
MlyI GAGTC 2 cut(s) 700, 850
MmeI TCCRAC 2 cut(s) 719, 1121
Mox20I TGGCCA 1 cut(s) 748
MscI TGGCCA 1 cut(s) 748
MseI TTAA 5 cut(s) 105, 273, 794, 819, 1194
Msp20I TGGCCA 1 cut(s) 748
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 1 cut(s) 141
MunI CAATTG 2 cut(s) 287, 1038
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 1 cut(s) 493
NdeI CATATG 1 cut(s) 901
NdeII GATC 3 cut(s) 253, 701, 770
NlaIII CATG 5 cut(s) 359, 503, 563, 624, 818
NlaIV GGNNCC 1 cut(s) 970
NmeAIII GCCGAG 1 cut(s) 53
NmuCI GTSAC 2 cut(s) 643, 850
NspI RCATGY 2 cut(s) 624, 818
PagI TCATGA 1 cut(s) 499
PfeI GAWTC 3 cut(s) 56, 133, 728
PleI GAGTC 2 cut(s) 699, 850
PpsI GAGTC 2 cut(s) 699, 850
PpuMI RGGWCCY 1 cut(s) 968
Psp124BI GAGCTC 2 cut(s) 27, 1145
Psp5II RGGWCCY 1 cut(s) 968
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 970
PspPI GGNCC 3 cut(s) 505, 968, 1094
PspPPI RGGWCCY 1 cut(s) 968
PstI CTGCAG 1 cut(s) 583
PsuI RGATCY 3 cut(s) 253, 701, 770
RsaI GTAC 4 cut(s) 179, 370, 928, 949
RsaNI GTAC 4 cut(s) 178, 369, 927, 948
SacI GAGCTC 2 cut(s) 27, 1145
SaqAI TTAA 5 cut(s) 105, 273, 794, 819, 1194
Sau3AI GATC 3 cut(s) 253, 701, 770
Sau96I GGNCC 3 cut(s) 505, 968, 1094
ScaI AGTACT 2 cut(s) 179, 949
SchI GAGTC 2 cut(s) 700, 850
ScrFI CCNGG 1 cut(s) 141
SduI GDGCHC 3 cut(s) 27, 148, 1145
SexAI ACCWGGT 1 cut(s) 139
SfaNI GCATC 3 cut(s) 202, 505, 722
SfcI CTRYAG 4 cut(s) 117, 528, 579, 732
SinI GGWCC 3 cut(s) 505, 968, 1094
SmlI CTYRAG 2 cut(s) 199, 344
SmoI CTYRAG 2 cut(s) 199, 344
SsiI CCGC 1 cut(s) 1186
SspMI CTAG 6 cut(s) 86, 395, 452, 633, 653, 768
SstI GAGCTC 2 cut(s) 27, 1145
StyD4I CCNGG 1 cut(s) 139
TaqI TCGA 2 cut(s) 21, 599
TatI WGTACW 3 cut(s) 177, 926, 947
TfiI GAWTC 3 cut(s) 56, 133, 728
Tru1I TTAA 5 cut(s) 105, 273, 794, 819, 1194
Tru9I TTAA 5 cut(s) 105, 273, 794, 819, 1194
TscAI CASTG 1 cut(s) 888
TseFI GTSAC 2 cut(s) 643, 850
Tsp45I GTSAC 2 cut(s) 643, 850
TspDTI ATGAA 4 cut(s) 372, 576, 849, 1076
TspGWI ACGGA 2 cut(s) 7, 32
TspRI CASTG 1 cut(s) 888
VpaK11BI GGWCC 3 cut(s) 505, 968, 1094
XapI RAATTY 4 cut(s) 127, 310, 410, 918
XbaI TCTAGA 2 cut(s) 652, 767
XceI RCATGY 2 cut(s) 624, 818
XspI CTAG 6 cut(s) 86, 395, 452, 633, 653, 768
ZrmI AGTACT 2 cut(s) 179, 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.