Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0003410

Physical Location & Seq

Genomic Coordinates Forward (+)

18140783 .. 18147506

Gene Structure 6724 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017550

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGACCTCCATCACCTCCGTCGAGCTCAATTTCCTCGTCTTCCGTTATCTTCAGGAATCAGGCTTCACACACTCGGCTTTTGCTCTAGGATATGAGGCCAGTATTAACAAATGCACTATAGATGGAAATTTGATTCCACCCGGTGCTCTCATTACATTTGTTCAAAAAGGGCTTCAGTACTTGGAGATGGAAGCCAACTTGAGTAATAGTGATGCAGAGGTGGATGAGGATTTCTCTCTTTTACAACCTTTAGATCTCATCCAAAATGATGTTAATCAACTACGCCAATTGATAAAAGATAGAAAGAAAAATTTGCAGAAGGATAGAGATAAGGACAAAGACCTTGAGAAAGAACATGAAGGAGAGCGTACACGAGTAAGAGAGAAGGAAAGACTAGAAAGGGAGAAGGAATTTGAAAAGAGCAGAGAGAGGGTAGAAAAGAATAAGGAGGTAGAAAAGAACAAGGAGCAAGAAAAGCATCATGAGGACCATAACGACAGAGATAGGGCTACAGAACAGGAAGAAAAAGTCAGTGTCAAACATGAAGAAAATGGAGCTTCTGCAGAACCAATGGATATCTCGACAGCTTCAACATCTCAGGCATGTGAAATCCCTAGTTCTGATGTGACCATTCTAGAAGGCCATACATCTGAGGTCTGTGCTTGTGCTTGGAGTCCAACTGGATCTCTTCTTGCTTCTGGGTCTGGAGATTCTACAGCTCGCATTTGGCCAATTCCAGATGGAAGCTCTAGATCTGGTTTACAAAATGGTGCTTTAAATGTGTTGGTGCTAAAGCATGTTAAGGGTAGGACAAATGAAAAGAGTAAAGATGTCACGACTCTTGATTGGAATGGAGAGGGAACACTGCTTGCAACTGGCTCATATGATGGGCAGGCAAGAATTTGGAGTACAAATGGTGAACTAAGGAGTACTTTGAACAAACATAAAGGTCCCATATTCTCTCTAAAGTGGAACAAGAAGGGAGATTATCTGCTTACGGGAAGCTGTGATAAAACTGCAATTGTGTGGGATGTGAAGGCAGAGGAAGTGAAGCAACAATTTGAGTTTCATTCAGGTCCAACACTTGATGTTGACTGGCGCAACAATATGCAATTTGCAATGAGCTCCACAGACAATATGATATATGTTTGCAAGATTGGAGAAAACCGCCCTATTAAAACTTTTGCAGGACATCAGGGTGAAGTTAATTGTGTGAAATGGGATCCAACTGGCACGCTGTTGGCTTCATGTTCTGATGATATCAGTGCAAAGATATGGAGCATGAAGCAGGAAAAGTATGTTCATGATTTAAGAGAGCATTCTAAGGAGATATACACTATCAGATGGAGTCCGACAGGACCAGGAACAAACAACCCTAACCAGCAGTTGGTTCTGGCAAGTGCGTCATTTGACTCTACTGTAAAGCTATGGGATGTGGAACTAGGAAAACTTCTCTACAGCTTGAACGGACACAGGGATCCTGTTTATTCCGTCGCCTTTAGCCCAAATGGTGAGTATTTGGCCAGTGGATCTCTTGATAAATCGATGCATATCTGGTCACTAAAGGAAGGCAAGATTGTGAAAACATACACCGGCAATGGCGGTATATTTGAAGTTTGCTGGAACAAGGAAGGTGACAAAATTGCTGCATGTTTTGCCAACAATACAGTCTGTGTTTTGGATTTCAGAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000118 GO:0000151 GO:0003674 GO:0003712 GO:0003714 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006950 GO:0006996 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031461 GO:0031974 GO:0031981 GO:0032991 GO:0035601 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0080090 GO:0098732 GO:0140110 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04013 ko04310 map04013 map04310

Pfam Domains

Protein Families

LisH WD40

Protein Analysis

565

Amino Acids

63.34

Weight (kDa)

5.53

Isoelectric Point (pI)

30.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH	PF08513	8 - 33	9.7e-08	LisH
WD40_CDC20-Fz	PF24807	208 - 246	1.3e-07	CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	208 - 316	2.7e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5	PF25175	208 - 250	2.4e-07	WDR5 beta-propeller domain
WD40	PF00400	209 - 243	2.3e-10	WD domain, G-beta repeat
Beta-prop_EML	PF23409	212 - 382	3.4e-10	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3	PF25174	215 - 247	3.3e-06	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	218 - 449	4.2e-08	MABP1/WDR62 second WD40 domain
Beta-prop_EML_2	PF23414	253 - 346	2.2e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_IFT140_1st	PF23383	266 - 347	4.8e-06	IFT140 first beta-propeller
WD40_Prp19	PF24814	269 - 352	1.1e-10	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	271 - 453	2.5e-13	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	271 - 371	6.4e-17	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	272 - 520	1.4e-11	WDR36/Utp21 first beta-propeller
Beta-prop_WDR5	PF25175	272 - 368	7.1e-17	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	275 - 356	2.1e-11	WDHD1 first WD40 domain
EIF3I	PF24805	275 - 381	6.3e-12	EIF3I
Beta-prop_THOC3	PF25174	275 - 511	3.4e-46	THOC3 beta-propeller domain
WD40	PF00400	275 - 303	4.1e-06	WD domain, G-beta repeat
NBCH_WD40	PF20426	276 - 439	5.4e-06	Neurobeachin beta propeller domain
WDR55	PF24796	276 - 366	8.1e-06	WDR55
Beta-prop_WDR3_2nd	PF25172	278 - 382	1.7e-07	WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	289 - 477	4.9e-11	TEP-1 second beta-propeller
WD40	PF00400	307 - 344	1.2e-07	WD domain, G-beta repeat
WD40_Gbeta	PF25391	308 - 432	1.3e-09	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	311 - 454	2.5e-23	CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	311 - 390	2.2e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin	PF25460	315 - 382	7.7e-06	Aladin seven-bladed propeller
WD40_Prp19	PF24814	319 - 559	3e-38	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	332 - 451	1.3e-10	Echinoderm microtubule-associated protein second beta-propeller
ANAPC4_WD40	PF12894	366 - 450	7.5e-08	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR3_1st	PF25173	367 - 480	1.4e-21	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	370 - 557	3.1e-27	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	371 - 562	9.1e-40	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	373 - 556	2.4e-15	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	375 - 548	2.4e-07	WDR36/Utp21 first beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	382 - 563	5.5e-09	MABP1/WDR62 second WD40 domain
WD40	PF00400	390 - 427	3.2e-10	WD domain, G-beta repeat
Beta-prop_EML	PF23409	395 - 529	4e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	396 - 528	2.6e-16	CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin	PF25460	401 - 453	8.1e-06	Aladin seven-bladed propeller
WDR90_beta-prop_4th	PF23342	402 - 526	7.7e-10	WDR90, 4th beta-propeller
WD40_MABP1-WDR62_1st	PF24780	408 - 529	3.4e-06	MABP1/WDR62 first WD40 domain
Beta-prop_WDR41	PF25178	413 - 537	4.9e-06	WDR41
WD40	PF00400	432 - 478	5e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	436 - 530	1.9e-10	CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2	PF23414	458 - 561	2.5e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_IFT122_1st	PF23381	461 - 554	2.9e-12	IFT122 first beta-propeller
Beta-prop_WDR3_1st	PF25173	461 - 557	3.2e-17	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	461 - 558	4.4e-11	CDC20/Fizzy WD40 domain
WDR55	PF24796	462 - 531	3e-06	WDR55
Beta-prop_TEP1_2nd	PF25047	463 - 524	6.9e-08	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	464 - 513	1.2e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd	PF23393	464 - 551	3.2e-06	WDR90/POC16, second beta-propeller
Beta-prop_THOC3	PF25174	464 - 563	2.9e-21	THOC3 beta-propeller domain
EIF3I	PF24805	464 - 553	3e-09	EIF3I
WD40_Gbeta	PF25391	467 - 563	3.3e-11	G protein beta WD-40 repeat protein
WD40	PF00400	482 - 520	5.8e-12	WD domain, G-beta repeat
Beta-prop_Aladin	PF25460	490 - 559	9.2e-07	Aladin seven-bladed propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67320
fragaria_vesca	FvH4_1g16940 FvH4_2g33540 FvH4_2g33540 FvH4_2g33540 FvH4_2g33540 FvH4_2g33540
malus_domestica	MD02G1179800.v1.1 MD15G1075300.v1.1 MD15G1290400.v1.1
prunus_persica	Prupe.1G428200_v2.0.a1 Prupe.1G428200_v2.0.a1 Prupe.6G226500_v2.0.a1 Prupe.6G226500_v2.0.a1
pyrus_communis	pycom02g14570 pycom15g07030 pycom15g25410
rosa_chinensis	RchiOBHm_Chr2g0107021 RchiOBHm_Chr6g0308951
rosa_laevigata	RLG00000010601 RLG00000017550
rosa_multiflora	Rmu_sc0007106.1_g000002 Rmu_sc0030997.1_g000001
rosa_roxburghii	Rroxscaffold_2G00136680 Rroxscaffold_7G00159780
rosa_rugosa	Rorug02G0140000
rosa_samantha	Rh2AG190800 Rh2BG202300 Rh2CG195500 Rh2DG197800 Rh6AG481200 Rh6BG490300 Rh6CG495600 Rh6DG481500
rosa_wichuraiana	Rw2G015040 Rw6G041920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1387
AciI	CCGC	2 cut(s)	1168, 1602
AclWI	GGATC	6 cut(s)	691, 1217, 1230, 1472, 1485, 1537
AcoI	YGGCCR	2 cut(s)	728, 1521
AcsI	RAATTY	4 cut(s)	127, 310, 410, 900
AcuI	CTGAAG	2 cut(s)	35, 158
AdeI	CACNNNGTG	1 cut(s)	143
AfaI	GTAC	4 cut(s)	179, 370, 910, 931
AfiI	CCNNNNNNNGG	1 cut(s)	1387
AgsI	TTSAA	6 cut(s)	164, 416, 591, 937, 1465, 1613
AjnI	CCWGG	1 cut(s)	1360
AluBI	AGCT	9 cut(s)	25, 557, 587, 719, 747, 1005, 1125, 1426, 1461
AluI	AGCT	9 cut(s)	25, 557, 587, 719, 747, 1005, 1125, 1426, 1461
Alw21I	GWGCWC	3 cut(s)	27, 148, 1127
AlwI	GGATC	6 cut(s)	691, 1217, 1230, 1472, 1485, 1537
AoxI	GGCC	4 cut(s)	96, 640, 728, 1521
ApeKI	GCWGC	1 cut(s)	1646
ApoI	RAATTY	4 cut(s)	127, 310, 410, 900
AspLEI	GCGC	1 cut(s)	1101
AspS9I	GGNCC	4 cut(s)	487, 950, 1076, 1358
AsuC2I	CCSGG	1 cut(s)	141
AsuHPI	GGTGA	5 cut(s)	4, 929, 1211, 1523, 1646
AvaII	GGWCC	4 cut(s)	487, 950, 1076, 1358
BalI	TGGCCA	2 cut(s)	730, 1523
BamHI	GGATCC	2 cut(s)	1222, 1477
BanII	GRGCYC	2 cut(s)	27, 1127
BauI	CACGAG	1 cut(s)	372
BbsI	GAAGAC	1 cut(s)	31
Bbv12I	GWGCWC	3 cut(s)	27, 148, 1127
BbvI	GCAGC	1 cut(s)	1633
BccI	CCATC	6 cut(s)	17, 116, 181, 734, 881, 1338
BciT130I	CCWGG	1 cut(s)	1362
BcnI	CCSGG	1 cut(s)	141
BfaI	CTAG	6 cut(s)	86, 395, 615, 635, 750, 1442
BfmI	CTRYAG	5 cut(s)	117, 510, 561, 714, 1456
BglII	AGATCT	2 cut(s)	253, 752
BisI	GCNGC	1 cut(s)	1647
BlsI	GCNGC	1 cut(s)	1648
BmcAI	AGTACT	2 cut(s)	179, 931
Bme1390I	CCNGG	2 cut(s)	141, 1362
Bme18I	GGWCC	4 cut(s)	487, 950, 1076, 1358
BmgT120I	GGNCC	4 cut(s)	487, 950, 1076, 1358
BmiI	GGNNCC	3 cut(s)	952, 1224, 1479
BmrFI	CCNGG	2 cut(s)	141, 1362
BmsI	GCATC	3 cut(s)	202, 487, 1536
BpiI	GAAGAC	1 cut(s)	31
BplI	GAGNNNNNCTC	2 cut(s)	218, 250
BpmI	CTGGAG	1 cut(s)	726
BpuEI	CTTGAG	2 cut(s)	220, 365
BpuMI	CCSGG	1 cut(s)	141
Bsa29I	ATCGAT	1 cut(s)	1544
BsaBI	GATNNNNATC	2 cut(s)	273, 1550
Bsc4I	CCNNNNNNNGG	1 cut(s)	1387
Bse118I	RCCGGY	1 cut(s)	1592
Bse1I	ACTGG	6 cut(s)	99, 685, 880, 1100, 1234, 1524
Bse3DI	GCAATG	2 cut(s)	1125, 1603
Bse8I	GATNNNNATC	2 cut(s)	273, 1550
BseBI	CCWGG	1 cut(s)	1362
BseCI	ATCGAT	1 cut(s)	1544
BseGI	GGATG	4 cut(s)	229, 258, 1036, 1438
BseJI	GATNNNNATC	2 cut(s)	273, 1550
BseLI	CCNNNNNNNGG	1 cut(s)	1387
BseMI	GCAATG	2 cut(s)	1125, 1603
BseMII	CTCAG	2 cut(s)	611, 642
BseNI	ACTGG	6 cut(s)	99, 685, 880, 1100, 1234, 1524
BseXI	GCAGC	1 cut(s)	1633
BshFI	GGCC	4 cut(s)	98, 642, 730, 1523
BshVI	ATCGAT	1 cut(s)	1544
BsiHKAI	GWGCWC	3 cut(s)	27, 148, 1127
BsiSI	CCGG	2 cut(s)	141, 1593
BslFI	GGGAC	1 cut(s)	936
BslI	CCNNNNNNNGG	1 cut(s)	1387
BsmFI	GGGAC	1 cut(s)	936
BsmI	GAATGC	1 cut(s)	1318
BsnI	GGCC	4 cut(s)	98, 642, 730, 1523
Bsp1286I	GDGCHC	3 cut(s)	27, 148, 1127
Bsp143I	GATC	6 cut(s)	253, 683, 752, 1222, 1477, 1529
BspACI	CCGC	2 cut(s)	1168, 1602
BspANI	GGCC	4 cut(s)	98, 642, 730, 1523
BspCNI	CTCAG	2 cut(s)	610, 643
BspDI	ATCGAT	1 cut(s)	1544
BspHI	TCATGA	2 cut(s)	481, 1303
BspLI	GGNNCC	3 cut(s)	952, 1224, 1479
BspMAI	CTGCAG	1 cut(s)	565
BspPI	GGATC	6 cut(s)	691, 1217, 1230, 1472, 1485, 1537
BsrDI	GCAATG	2 cut(s)	1125, 1603
BsrFI	RCCGGY	1 cut(s)	1592
BsrI	ACTGG	6 cut(s)	99, 685, 880, 1100, 1234, 1524
BssAI	RCCGGY	1 cut(s)	1592
BssMI	GATC	6 cut(s)	253, 683, 752, 1222, 1477, 1529
BssSI	CACGAG	1 cut(s)	372
Bst2BI	CACGAG	1 cut(s)	372
Bst2UI	CCWGG	1 cut(s)	1362
Bst4CI	ACNGT	2 cut(s)	1420, 1669
Bst6I	CTCTTC	1 cut(s)	693
BstAPI	GCANNNNNTGC	1 cut(s)	1655
BstC8I	GCNNGC	4 cut(s)	721, 870, 894, 1235
BstDEI	CTNAG	4 cut(s)	597, 651, 923, 1323
BstF5I	GGATG	4 cut(s)	229, 258, 1036, 1438
BstHHI	GCGC	1 cut(s)	1101
BstKTI	GATC	6 cut(s)	256, 686, 755, 1225, 1480, 1532
BstMBI	GATC	6 cut(s)	253, 683, 752, 1222, 1477, 1529
BstMWI	GCNNNNNNNGC	2 cut(s)	475, 1655
BstNI	CCWGG	1 cut(s)	1362
BstNSI	RCATGY	3 cut(s)	606, 800, 1653
BstSCI	CCNGG	2 cut(s)	139, 1360
BstSFI	CTRYAG	5 cut(s)	117, 510, 561, 714, 1456
BstV1I	GCAGC	1 cut(s)	1633
BstV2I	GAAGAC	1 cut(s)	31
BstX2I	RGATCY	6 cut(s)	253, 683, 752, 1222, 1477, 1529
BstYI	RGATCY	6 cut(s)	253, 683, 752, 1222, 1477, 1529
Bsu15I	ATCGAT	1 cut(s)	1544
BsuRI	GGCC	4 cut(s)	98, 642, 730, 1523
BsuTUI	ATCGAT	1 cut(s)	1544
BtsCI	GGATG	4 cut(s)	229, 258, 1036, 1438
BtsI	GCAGTG	1 cut(s)	863
BtsIMutI	CAGTG	4 cut(s)	538, 863, 1270, 1531
Cac8I	GCNNGC	4 cut(s)	721, 870, 894, 1235
CciI	TCATGA	2 cut(s)	481, 1303
CfoI	GCGC	1 cut(s)	1101
Cfr10I	RCCGGY	1 cut(s)	1592
Cfr13I	GGNCC	4 cut(s)	487, 950, 1076, 1358
ClaI	ATCGAT	1 cut(s)	1544
CseI	GACGC	1 cut(s)	1392
Csp6I	GTAC	4 cut(s)	178, 369, 909, 930
CviAII	CATG	9 cut(s)	356, 482, 542, 603, 797, 1248, 1282, 1304, 1650
CviQI	GTAC	4 cut(s)	178, 369, 909, 930
DdeI	CTNAG	4 cut(s)	597, 651, 923, 1323
DpnI	GATC	6 cut(s)	255, 685, 754, 1224, 1479, 1531
DpnII	GATC	6 cut(s)	253, 683, 752, 1222, 1477, 1529
DraI	TTTAAA	1 cut(s)	777
DraIII	CACNNNGTG	1 cut(s)	143
EaeI	YGGCCR	2 cut(s)	728, 1521
Eam1104I	CTCTTC	1 cut(s)	693
EarI	CTCTTC	1 cut(s)	693
Ecl136II	GAGCTC	2 cut(s)	25, 1125
Eco24I	GRGCYC	2 cut(s)	27, 1127
Eco32I	GATATC	2 cut(s)	577, 1261
Eco47I	GGWCC	4 cut(s)	487, 950, 1076, 1358
Eco53kI	GAGCTC	2 cut(s)	25, 1125
Eco57I	CTGAAG	2 cut(s)	35, 158
EcoICRI	GAGCTC	2 cut(s)	25, 1125
EcoO109I	RGGNCCY	1 cut(s)	950
EcoRII	CCWGG	1 cut(s)	1360
EcoRV	GATATC	2 cut(s)	577, 1261
EcoT22I	ATGCAT	1 cut(s)	1551
EcoT38I	GRGCYC	2 cut(s)	27, 1127
FaeI	CATG	9 cut(s)	359, 485, 545, 606, 800, 1251, 1285, 1307, 1653
FalI	AAGNNNNNCTT	2 cut(s)	916, 948
FaqI	GGGAC	1 cut(s)	936
FatI	CATG	9 cut(s)	355, 481, 541, 602, 796, 1247, 1281, 1303, 1649
FauNDI	CATATG	1 cut(s)	883
Fnu4HI	GCNGC	1 cut(s)	1647
FokI	GGATG	4 cut(s)	236, 245, 1043, 1445
FriOI	GRGCYC	2 cut(s)	27, 1127
Fsp4HI	GCNGC	1 cut(s)	1647
FspBI	CTAG	6 cut(s)	86, 395, 615, 635, 750, 1442
GlaI	GCGC	1 cut(s)	1100
GluI	GCNGC	1 cut(s)	1647
GsuI	CTGGAG	1 cut(s)	726
HaeIII	GGCC	4 cut(s)	98, 642, 730, 1523
HapII	CCGG	2 cut(s)	141, 1593
HgaI	GACGC	1 cut(s)	1392
HhaI	GCGC	1 cut(s)	1101
Hin1II	CATG	9 cut(s)	359, 485, 545, 606, 800, 1251, 1285, 1307, 1653
Hin6I	GCGC	1 cut(s)	1099
HinP1I	GCGC	1 cut(s)	1099
HincII	GTYRAC	1 cut(s)	1093
HindII	GTYRAC	1 cut(s)	1093
HinfI	GANTC	7 cut(s)	56, 133, 673, 710, 838, 1348, 1412
HpaII	CCGG	2 cut(s)	141, 1593
HphI	GGTGA	5 cut(s)	4, 929, 1211, 1523, 1646
Hpy166II	GTNNAC	4 cut(s)	371, 761, 920, 1093
Hpy188I	TCNGA	6 cut(s)	622, 652, 1255, 1343, 1353, 1688
Hpy8I	GTNNAC	4 cut(s)	371, 761, 920, 1093
Hpy99I	CGWCG	2 cut(s)	23, 1496
HpyAV	CCTTC	9 cut(s)	313, 353, 379, 400, 632, 973, 1030, 1562, 1625
HpyCH4III	ACNGT	2 cut(s)	1420, 1669
HpyF10VI	GCNNNNNNNGC	2 cut(s)	475, 1655
HpyF3I	CTNAG	4 cut(s)	597, 651, 923, 1323
Hsp92II	CATG	9 cut(s)	359, 485, 545, 606, 800, 1251, 1285, 1307, 1653
HspAI	GCGC	1 cut(s)	1099
Kzo9I	GATC	6 cut(s)	253, 683, 752, 1222, 1477, 1529
LmnI	GCTCC	4 cut(s)	466, 554, 1130, 1278
Lsp1109I	GCAGC	1 cut(s)	1633
LweI	GCATC	3 cut(s)	202, 487, 1536
MaeI	CTAG	6 cut(s)	86, 395, 615, 635, 750, 1442
MaeIII	GTNAC	4 cut(s)	625, 832, 1557, 1634
MalI	GATC	6 cut(s)	255, 685, 754, 1224, 1479, 1531
MboI	GATC	6 cut(s)	253, 683, 752, 1222, 1477, 1529
MboII	GAAGA	5 cut(s)	31, 41, 533, 557, 680
MfeI	CAATTG	2 cut(s)	287, 1020
MflI	RGATCY	6 cut(s)	253, 683, 752, 1222, 1477, 1529
MhlI	GDGCHC	3 cut(s)	27, 148, 1127
MlsI	TGGCCA	2 cut(s)	730, 1523
MluNI	TGGCCA	2 cut(s)	730, 1523
MlyI	GAGTC	4 cut(s)	682, 832, 1357, 1406
MmeI	TCCRAC	4 cut(s)	701, 1103, 1250, 1376
Mox20I	TGGCCA	2 cut(s)	730, 1523
Mph1103I	ATGCAT	1 cut(s)	1551
MscI	TGGCCA	2 cut(s)	730, 1523
MseI	TTAA	7 cut(s)	105, 273, 776, 801, 1176, 1206, 1310
MslI	CAYNNNNRTG	1 cut(s)	1197
Msp20I	TGGCCA	2 cut(s)	730, 1523
MspI	CCGG	2 cut(s)	141, 1593
MspR9I	CCNGG	2 cut(s)	141, 1362
MunI	CAATTG	2 cut(s)	287, 1020
Mva1269I	GAATGC	1 cut(s)	1318
MvaI	CCWGG	1 cut(s)	1362
MwoI	GCNNNNNNNGC	2 cut(s)	475, 1655
NciI	CCSGG	1 cut(s)	141
NdeI	CATATG	1 cut(s)	883
NdeII	GATC	6 cut(s)	253, 683, 752, 1222, 1477, 1529
NlaIII	CATG	9 cut(s)	359, 485, 545, 606, 800, 1251, 1285, 1307, 1653
NlaIV	GGNNCC	3 cut(s)	952, 1224, 1479
NmeAIII	GCCGAG	1 cut(s)	53
NmuCI	GTSAC	4 cut(s)	625, 832, 1557, 1634
NsiI	ATGCAT	1 cut(s)	1551
NspI	RCATGY	3 cut(s)	606, 800, 1653
PagI	TCATGA	2 cut(s)	481, 1303
PctI	GAATGC	1 cut(s)	1318
PfeI	GAWTC	3 cut(s)	56, 133, 710
PflMI	CCANNNNNTGG	1 cut(s)	1387
PkrI	GCNGC	1 cut(s)	1648
PleI	GAGTC	4 cut(s)	681, 832, 1356, 1406
PpsI	GAGTC	4 cut(s)	681, 832, 1356, 1406
PpuMI	RGGWCCY	1 cut(s)	950
Psp124BI	GAGCTC	2 cut(s)	27, 1127
Psp5II	RGGWCCY	1 cut(s)	950
Psp6I	CCWGG	1 cut(s)	1360
PspGI	CCWGG	1 cut(s)	1360
PspN4I	GGNNCC	3 cut(s)	952, 1224, 1479
PspPI	GGNCC	4 cut(s)	487, 950, 1076, 1358
PspPPI	RGGWCCY	1 cut(s)	950
PstI	CTGCAG	1 cut(s)	565
PsuI	RGATCY	6 cut(s)	253, 683, 752, 1222, 1477, 1529
RsaI	GTAC	4 cut(s)	179, 370, 910, 931
RsaNI	GTAC	4 cut(s)	178, 369, 909, 930
RseI	CAYNNNNRTG	1 cut(s)	1197
SacI	GAGCTC	2 cut(s)	27, 1127
SaqAI	TTAA	7 cut(s)	105, 273, 776, 801, 1176, 1206, 1310
SatI	GCNGC	1 cut(s)	1647
Sau3AI	GATC	6 cut(s)	253, 683, 752, 1222, 1477, 1529
Sau96I	GGNCC	4 cut(s)	487, 950, 1076, 1358
ScaI	AGTACT	2 cut(s)	179, 931
SchI	GAGTC	4 cut(s)	682, 832, 1357, 1406
ScrFI	CCNGG	2 cut(s)	141, 1362
SduI	GDGCHC	3 cut(s)	27, 148, 1127
SfaNI	GCATC	3 cut(s)	202, 487, 1536
SfcI	CTRYAG	5 cut(s)	117, 510, 561, 714, 1456
SinI	GGWCC	4 cut(s)	487, 950, 1076, 1358
SmiMI	CAYNNNNRTG	1 cut(s)	1197
SmlI	CTYRAG	2 cut(s)	199, 344
SmoI	CTYRAG	2 cut(s)	199, 344
SsiI	CCGC	2 cut(s)	1168, 1602
SspMI	CTAG	6 cut(s)	86, 395, 615, 635, 750, 1442
SstI	GAGCTC	2 cut(s)	27, 1127
StyD4I	CCNGG	2 cut(s)	139, 1360
TaaI	ACNGT	2 cut(s)	1420, 1669
TaqI	TCGA	3 cut(s)	21, 581, 1544
TatI	WGTACW	3 cut(s)	177, 908, 929
TfiI	GAWTC	3 cut(s)	56, 133, 710
Tru1I	TTAA	7 cut(s)	105, 273, 776, 801, 1176, 1206, 1310
Tru9I	TTAA	7 cut(s)	105, 273, 776, 801, 1176, 1206, 1310
TscAI	CASTG	4 cut(s)	538, 870, 1270, 1531
TseFI	GTSAC	4 cut(s)	625, 832, 1557, 1634
TseI	GCWGC	1 cut(s)	1646
Tsp45I	GTSAC	4 cut(s)	625, 832, 1557, 1634
TspDTI	ATGAA	7 cut(s)	372, 558, 831, 1058, 1236, 1292, 1298
TspGWI	ACGGA	4 cut(s)	7, 32, 1480, 1482
TspRI	CASTG	4 cut(s)	538, 870, 1270, 1531
Van91I	CCANNNNNTGG	1 cut(s)	1387
VpaK11BI	GGWCC	4 cut(s)	487, 950, 1076, 1358
XapI	RAATTY	4 cut(s)	127, 310, 410, 900
XbaI	TCTAGA	2 cut(s)	634, 749
XceI	RCATGY	3 cut(s)	606, 800, 1653
XspI	CTAG	6 cut(s)	86, 395, 615, 635, 750, 1442
ZrmI	AGTACT	2 cut(s)	179, 931
Zsp2I	ATGCAT	1 cut(s)	1551

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.