Rh6CG495600

F-box-like WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0003410
Physical Location & Seq
Forward (+)
66132026 .. 66135502
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG495600.1

Sequence Viewer

Length: 1629 bp
ATGATCTCCATCAACTCCACCGAATTGAACTACCTGGTCTTCCGTTACCTCCATGAATCAGGCTTCACACATTCAGCTTATGCTTTCGGATTCGAGGCGGGGATCAACAAGAGCACGGTTGATGGTAACCTAGTTCCGCCTGGTGCACTTGTTACGTTTCTCCAGAAAGGCCTTCAGTTTCTTGAGCTGGAGGCGGACTTAGGCAATGGTAGTGATGCGGATATGGAGGATGATTTTTCGTTCCTGCAACCGTTGGATTTTATCACAAAAGATGCGTATCAATTGCAGAAAATTGTTAGGGAAAAGAAGGATAGGATGAATGCTGCTAACAATGAGTCCAAGCAGGAAAGCGAAAGGGAAGTAGAGGTGGAGAGAAAAGAAGAAGAGATTCGAGACGATAAGTCGGAGAAGGATAGGGAGCAGGAGATTGGTAAAGAGGAGGAGAAGTCGAATCAGGATCGAAGTGAGATGGAGCCTGAAGAAAATGGAACTGGTGGAGGGGTGGAGCCAATGGAGACCTCCCCGAGCTCAACGTTCGTGCCTGGTGAAATTCCTATTAGTGATGTCATAGTTCTAGAAGGGCATACCTCAGAGGTTTTTGCTTGTGCATGGAGTCCATCTGGTTCGCTTCTTGCATCTGGTTCTGGGGATTCAACAGCTCGAATTTGGACCATTGCCAGTGGAAGTTGTAGATCTAGCATTCAAAATGAACCTGCACGTGTGTTAAAGCATTTCAAAGGGAGAGCCAATGAGAAAAGCAAGGATGTGACTACACTTGATTGGAATGATGATGGAACGTTACTAGCAACAGGTTCCTATGATGGGCAAGCAAGAATATGGGGTAGAGACGGAGAGTTAATGACTACTTTGATCAGACATAAAGGGCCAATCTTTTCTCTGAAGTGGAACAAAGAAGGTGATTATCTTCTGAGTGGAAGTGTGGATAAAACTGCAATTGTGTGGGACATAAAGACTGGCGAATGGAAACAACAGTTTGAATTTCATTCAGCTCCAACTCTGGATGTTGATTGGCGAGACAATACTTCTTTTGCAACATGCTCTACTGATGGTACGATACATGTTTGCAAGGTTGGAGAGAACCAACCAATTAAAACTTTCTTAGGCCATCAGGGTGAAGTTAATGCTATTAAGTGGGATCCGACTGGCTCATTATTGGCTTCTTGCTCTGATGATCATACAGCAAAGATATGGAGTATGAAGCAAGACAAATATTTGCATGATTTGAAGGAACATGTAAAGGAGATATATACCATCCGGTGGAGTCCTACAGGACCTGGCTCACATAATCCAAATCAACAATTAGTGCTAGCAAGCGCTTCCTTTGATTCACGCATAAAACTATGGGATGTGGAACTAGGGACACTTATCTACAGCTTATATGGCCACAGGGATCCGGTATACTCTGTTGCATTCAGCCCTAATGGTGAGTACTTGGCCAGTGGATCTATGGACAAATGCATGCACATATGGTCTGTGAAGGAAGGCAGAATCGTAAAAACATACACTGGAAATGGTGGAATATTCGAAGTTTGTTGGAACAAAGACGGCGATAAGATAGCTGCTTGTTTCGCTAACAATAATGTATGTGTTGTGGACTTCCGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0001067 GO:0002021 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006508 GO:0006511 GO:0006629 GO:0006807 GO:0006996 GO:0007275 GO:0008013 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009888 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010647 GO:0015630 GO:0016042 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0017053 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0023051 GO:0023056 GO:0030111 GO:0030154 GO:0030163 GO:0030177 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031667 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0035264 GO:0035601 GO:0036211 GO:0040007 GO:0042393 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044212 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0045444 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0047485 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050872 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051603 GO:0060255 GO:0060612 GO:0060613 GO:0060828 GO:0061448 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090207 GO:0090263 GO:0097159 GO:0098732 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140110 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04013 ko04310 map04013 map04310
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

542

Amino Acids

60.46

Weight (kDa)

4.95

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH PF08513 8 - 32 1.8e-06 LisH
Beta-prop_TEP1_2nd PF25047 186 - 281 7.3e-06 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 187 - 235 6e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 190 - 236 9.5e-10 WDR5 beta-propeller domain
WD40 PF00400 190 - 223 4.4e-10 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 190 - 235 1.9e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 190 - 237 3e-06 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 191 - 283 1.9e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 192 - 235 1.6e-07 THOC3 beta-propeller domain
Beta-prop_EML PF23409 193 - 327 1.3e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 198 - 327 4.3e-10 Aladin seven-bladed propeller
WD40_MABP1-WDR62_2nd PF24782 247 - 498 3.4e-13 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 248 - 346 7.1e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 249 - 330 2.5e-10 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 250 - 373 2.4e-23 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 250 - 344 1.7e-14 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 252 - 498 2.6e-12 WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st PF24817 253 - 335 1.8e-11 WDHD1 first WD40 domain
WD40 PF00400 253 - 280 3e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 253 - 431 1.5e-30 THOC3 beta-propeller domain
EIF3I PF24805 254 - 356 3.3e-11 EIF3I
WDR55 PF24796 255 - 413 2.6e-13 WDR55
Beta-prop_WDR3_2nd PF25172 256 - 359 9.4e-09 WDR3 second beta-propeller domain
Beta-prop_WDR19_1st PF23389 259 - 356 4.6e-06 WDR19 first beta-propeller
WD40 PF00400 285 - 322 1.7e-07 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 288 - 455 5.3e-13 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 289 - 368 2.8e-09 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 290 - 465 6.6e-28 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 293 - 431 8.3e-12 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 299 - 537 7.2e-37 Prp19 WD40 domain
WD40_Gbeta PF25391 307 - 412 3.4e-12 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 341 - 536 2.8e-29 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 345 - 458 1.1e-20 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 346 - 429 6.3e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 346 - 540 8.7e-41 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 346 - 541 5e-09 MABP1/WDR62 second WD40 domain
Beta-prop_Vps41 PF23411 348 - 412 8.4e-06 Vps42 beta-propeller
ANAPC4_WD40 PF12894 351 - 428 3.4e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR3_2nd PF25172 352 - 532 2.2e-16 WDR3 second beta-propeller domain
WD40 PF00400 368 - 405 4.2e-11 WD domain, G-beta repeat
EIF3I PF24805 373 - 465 9.9e-07 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 375 - 506 3.1e-14 CAF1B/HIR1 beta-propeller domain
WDR90_beta-prop_4th PF23342 380 - 506 1.1e-08 WDR90, 4th beta-propeller
WD40_MABP1-WDR62_1st PF24780 386 - 522 4.8e-06 MABP1/WDR62 first WD40 domain
Beta-prop_SCAP PF24017 394 - 500 8.9e-06 SCAP Beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 414 - 507 4.6e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 436 - 538 7.8e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_IFT122_1st PF23381 439 - 531 7e-13 IFT122 first beta-propeller
Beta-prop_WDR3_1st PF25173 439 - 535 8.7e-17 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 440 - 538 2.3e-10 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 441 - 502 9.3e-08 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 442 - 541 5e-20 THOC3 beta-propeller domain
EIF3I PF24805 442 - 530 2.8e-08 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 443 - 491 2.9e-06 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 445 - 541 1.4e-09 G protein beta WD-40 repeat protein
WD40 PF00400 460 - 498 7.6e-12 WD domain, G-beta repeat
WD40_RFWD3 PF23419 469 - 541 4.6e-06 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_Aladin PF25460 470 - 532 2.6e-07 Aladin seven-bladed propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 721
AccB7I CCANNNNNTGG 1 cut(s) 1278
AccI GTMKAC 1 cut(s) 1419
AciI CCGC 4 cut(s) 98, 137, 194, 218
AclI AACGTT 2 cut(s) 533, 797
AclWI GGATC 7 cut(s) 110, 465, 1151, 1164, 1406, 1419, 1471
AcoI YGGCCR 2 cut(s) 1402, 1455
AcsI RAATTY 3 cut(s) 549, 663, 998
AcuI CTGAAG 3 cut(s) 158, 498, 920
AcvI CACGTG 1 cut(s) 719
AfaI GTAC 2 cut(s) 1072, 1451
AfeI AGCGCT 1 cut(s) 1336
AfiI CCNNNNNNNGG 2 cut(s) 822, 1278
AflIII ACRYGT 3 cut(s) 718, 1078, 1252
AgsI TTSAA 6 cut(s) 28, 654, 704, 736, 998, 1246
AhdI GACNNNNNGTC 1 cut(s) 400
AjnI CCWGG 4 cut(s) 33, 139, 541, 1294
AluBI AGCT 7 cut(s) 77, 187, 528, 659, 1010, 1395, 1580
AluI AGCT 7 cut(s) 77, 187, 528, 659, 1010, 1395, 1580
Alw21I GWGCWC 3 cut(s) 116, 148, 530
Alw26I GTCTC 4 cut(s) 387, 509, 840, 1029
Alw44I GTGCAC 1 cut(s) 144
AlwI GGATC 7 cut(s) 110, 465, 1151, 1164, 1406, 1419, 1471
AlwNI CAGNNNCTG 1 cut(s) 1295
Ama87I CYCGRG 1 cut(s) 523
Aor51HI AGCGCT 1 cut(s) 1336
AoxI GGCC 5 cut(s) 169, 884, 1123, 1402, 1455
ApaLI GTGCAC 1 cut(s) 144
ApeKI GCWGC 2 cut(s) 323, 1580
ApoI RAATTY 3 cut(s) 549, 663, 998
Asp700I GAANNNNTTC 1 cut(s) 387
AspLEI GCGC 1 cut(s) 1337
AspS9I GGNCC 3 cut(s) 669, 884, 1292
AsuHPI GGTGA 4 cut(s) 557, 929, 1145, 1457
AsuII TTCGAA 1 cut(s) 1545
AsuNHI GCTAGC 1 cut(s) 1327
AvaI CYCGRG 1 cut(s) 523
AvaII GGWCC 2 cut(s) 669, 1292
BaeGI GKGCMC 1 cut(s) 148
BalI TGGCCA 2 cut(s) 1404, 1457
BamHI GGATCC 2 cut(s) 1156, 1411
BanII GRGCYC 1 cut(s) 530
BbrPI CACGTG 1 cut(s) 719
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 3 cut(s) 116, 148, 530
BbvI GCAGC 2 cut(s) 310, 1567
BccI CCATC 9 cut(s) 17, 116, 463, 625, 785, 815, 1061, 1134, 1280
BceAI ACGGC 1 cut(s) 1582
BciT130I CCWGG 4 cut(s) 35, 141, 543, 1296
BclI TGATCA 2 cut(s) 870, 1192
BcoDI GTCTC 4 cut(s) 387, 509, 840, 1029
BfaI CTAG 6 cut(s) 131, 575, 696, 803, 1328, 1376
BfmI CTRYAG 2 cut(s) 1287, 1390
BfoI RGCGCY 1 cut(s) 1338
BfuAI ACCTGC 1 cut(s) 721
BglII AGATCT 1 cut(s) 692
BisI GCNGC 2 cut(s) 324, 1581
BlsI GCNGC 2 cut(s) 325, 1582
BmcAI AGTACT 1 cut(s) 1451
Bme1390I CCNGG 4 cut(s) 35, 141, 543, 1296
Bme18I GGWCC 2 cut(s) 669, 1292
BmeRI GACNNNNNGTC 1 cut(s) 400
BmeT110I CYCGRG 1 cut(s) 523
BmgT120I GGNCC 3 cut(s) 669, 884, 1292
BmiI GGNNCC 5 cut(s) 474, 507, 814, 1158, 1413
BmrFI CCNGG 4 cut(s) 35, 141, 543, 1296
BmsI GCATC 3 cut(s) 205, 262, 644
BmtI GCTAGC 1 cut(s) 1331
BpiI GAAGAC 1 cut(s) 31
BpmI CTGGAG 2 cut(s) 146, 209
Bpu14I TTCGAA 1 cut(s) 1545
BpuEI CTTGAG 1 cut(s) 203
BsaAI YACGTR 1 cut(s) 719
BsaBI GATNNNNATC 2 cut(s) 8, 276
BsaI GGTCTC 1 cut(s) 509
BsaWI WCCGGW 2 cut(s) 1275, 1414
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 2 cut(s) 822, 1278
Bse1I ACTGG 6 cut(s) 496, 678, 979, 1168, 1458, 1531
Bse3DI GCAATG 2 cut(s) 211, 672
Bse8I GATNNNNATC 2 cut(s) 8, 276
BseBI CCWGG 4 cut(s) 35, 141, 543, 1296
BseGI GGATG 6 cut(s) 235, 321, 769, 1027, 1272, 1372
BseJI GATNNNNATC 2 cut(s) 8, 276
BseLI CCNNNNNNNGG 2 cut(s) 822, 1278
BseMI GCAATG 2 cut(s) 211, 672
BseMII CTCAG 2 cut(s) 603, 920
BseNI ACTGG 6 cut(s) 496, 678, 979, 1168, 1458, 1531
BseRI GAGGAG 2 cut(s) 452, 455
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 2 cut(s) 310, 1567
BsgI GTGCAG 1 cut(s) 699
BshFI GGCC 5 cut(s) 171, 886, 1125, 1404, 1457
BsiHKAI GWGCWC 3 cut(s) 116, 148, 530
BsiHKCI CYCGRG 1 cut(s) 523
BsiSI CCGG 2 cut(s) 1276, 1415
BslFI GGGAC 2 cut(s) 977, 1393
BslI CCNNNNNNNGG 2 cut(s) 822, 1278
BsmAI GTCTC 4 cut(s) 387, 509, 840, 1029
BsmBI CGTCTC 2 cut(s) 387, 840
BsmFI GGGAC 2 cut(s) 977, 1393
BsmI GAATGC 3 cut(s) 325, 699, 1430
BsnI GGCC 5 cut(s) 171, 886, 1125, 1404, 1457
Bso31I GGTCTC 1 cut(s) 509
BsoBI CYCGRG 1 cut(s) 523
Bsp119I TTCGAA 1 cut(s) 1545
Bsp1286I GDGCHC 3 cut(s) 116, 148, 530
Bsp143I GATC 9 cut(s) 3, 102, 457, 692, 870, 1156, 1192, 1411, 1463
BspACI CCGC 4 cut(s) 98, 137, 194, 218
BspANI GGCC 5 cut(s) 171, 886, 1125, 1404, 1457
BspCNI CTCAG 2 cut(s) 602, 921
BspLI GGNNCC 5 cut(s) 474, 507, 814, 1158, 1413
BspMI ACCTGC 1 cut(s) 721
BspOI GCTAGC 1 cut(s) 1331
BspPI GGATC 7 cut(s) 110, 465, 1151, 1164, 1406, 1419, 1471
BspT104I TTCGAA 1 cut(s) 1545
BspTNI GGTCTC 1 cut(s) 509
BsrDI GCAATG 2 cut(s) 211, 672
BsrI ACTGG 6 cut(s) 496, 678, 979, 1168, 1458, 1531
BssMI GATC 9 cut(s) 3, 102, 457, 692, 870, 1156, 1192, 1411, 1463
BssNAI GTATAC 1 cut(s) 1420
Bst1107I GTATAC 1 cut(s) 1420
Bst2UI CCWGG 4 cut(s) 35, 141, 543, 1296
Bst4CI ACNGT 3 cut(s) 118, 252, 993
Bst6I CTCTTC 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 719
BstBI TTCGAA 1 cut(s) 1545
BstC8I GCNNGC 4 cut(s) 828, 1329, 1333, 1481
BstDEI CTNAG 4 cut(s) 199, 589, 929, 1120
BstEII GGTNACC 1 cut(s) 125
BstF5I GGATG 6 cut(s) 235, 321, 769, 1027, 1272, 1372
BstH2I RGCGCY 1 cut(s) 1338
BstHHI GCGC 1 cut(s) 1337
BstKTI GATC 9 cut(s) 6, 105, 460, 695, 873, 1159, 1195, 1414, 1466
BstMAI GTCTC 4 cut(s) 387, 509, 840, 1029
BstMBI GATC 9 cut(s) 3, 102, 457, 692, 870, 1156, 1192, 1411, 1463
BstMWI GCNNNNNNNGC 2 cut(s) 1401, 1589
BstNI CCWGG 4 cut(s) 35, 141, 543, 1296
BstNSI RCATGY 4 cut(s) 1059, 1082, 1256, 1483
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 4 cut(s) 33, 139, 541, 1294
BstSFI CTRYAG 2 cut(s) 1287, 1390
BstSLI GKGCMC 1 cut(s) 148
BstV1I GCAGC 2 cut(s) 310, 1567
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 4 cut(s) 692, 1156, 1411, 1463
BstYI RGATCY 4 cut(s) 692, 1156, 1411, 1463
BstZ17I GTATAC 1 cut(s) 1420
BsuRI GGCC 5 cut(s) 171, 886, 1125, 1404, 1457
BtsCI GGATG 6 cut(s) 235, 321, 769, 1027, 1272, 1372
BtsIMutI CAGTG 3 cut(s) 685, 1465, 1524
BveI ACCTGC 1 cut(s) 721
Cac8I GCNNGC 4 cut(s) 828, 1329, 1333, 1481
CaiI CAGNNNCTG 1 cut(s) 1295
CfoI GCGC 1 cut(s) 1337
Cfr13I GGNCC 3 cut(s) 669, 884, 1292
CsiI ACCWGGT 1 cut(s) 33
Csp6I GTAC 2 cut(s) 1071, 1450
CviAII CATG 7 cut(s) 53, 609, 1056, 1079, 1238, 1253, 1480
CviQI GTAC 2 cut(s) 1071, 1450
DdeI CTNAG 4 cut(s) 199, 589, 929, 1120
DpnI GATC 9 cut(s) 5, 104, 459, 694, 872, 1158, 1194, 1413, 1465
DpnII GATC 9 cut(s) 3, 102, 457, 692, 870, 1156, 1192, 1411, 1463
DriI GACNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 2 cut(s) 1402, 1455
Eam1104I CTCTTC 1 cut(s) 378
Eam1105I GACNNNNNGTC 1 cut(s) 400
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 2 cut(s) 126, 209
Ecl136II GAGCTC 1 cut(s) 528
Eco147I AGGCCT 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 530
Eco31I GGTCTC 1 cut(s) 509
Eco47I GGWCC 2 cut(s) 669, 1292
Eco47III AGCGCT 1 cut(s) 1336
Eco53kI GAGCTC 1 cut(s) 528
Eco57I CTGAAG 3 cut(s) 158, 498, 920
Eco72I CACGTG 1 cut(s) 719
Eco88I CYCGRG 1 cut(s) 523
Eco91I GGTNACC 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 528
EcoO109I RGGNCCY 1 cut(s) 1292
EcoO65I GGTNACC 1 cut(s) 125
EcoRII CCWGG 4 cut(s) 33, 139, 541, 1294
EcoT22I ATGCAT 1 cut(s) 1481
EcoT38I GRGCYC 1 cut(s) 530
Esp3I CGTCTC 2 cut(s) 387, 840
FaeI CATG 7 cut(s) 56, 612, 1059, 1082, 1241, 1256, 1483
FaqI GGGAC 2 cut(s) 977, 1393
FatI CATG 7 cut(s) 52, 608, 1055, 1078, 1237, 1252, 1479
FauI CCCGC 1 cut(s) 91
FauNDI CATATG 1 cut(s) 1487
FbaI TGATCA 2 cut(s) 870, 1192
FblI GTMKAC 1 cut(s) 1419
Fnu4HI GCNGC 2 cut(s) 324, 1581
FokI GGATG 6 cut(s) 242, 328, 776, 1034, 1259, 1379
FriOI GRGCYC 1 cut(s) 530
Fsp4HI GCNGC 2 cut(s) 324, 1581
FspBI CTAG 6 cut(s) 131, 575, 696, 803, 1328, 1376
GlaI GCGC 1 cut(s) 1336
GluI GCNGC 2 cut(s) 324, 1581
GsuI CTGGAG 2 cut(s) 146, 209
HaeII RGCGCY 1 cut(s) 1338
HaeIII GGCC 5 cut(s) 171, 886, 1125, 1404, 1457
HapII CCGG 2 cut(s) 1276, 1415
HhaI GCGC 1 cut(s) 1337
Hin1II CATG 7 cut(s) 56, 612, 1059, 1082, 1241, 1256, 1483
Hin6I GCGC 1 cut(s) 1335
HinP1I GCGC 1 cut(s) 1335
HpaII CCGG 2 cut(s) 1276, 1415
HphI GGTGA 4 cut(s) 557, 929, 1145, 1457
Hpy166II GTNNAC 3 cut(s) 146, 1420, 1615
Hpy188I TCNGA 9 cut(s) 89, 406, 592, 875, 900, 930, 1161, 1189, 1622
Hpy188III TCNNGA 6 cut(s) 163, 182, 392, 455, 575, 1019
Hpy8I GTNNAC 3 cut(s) 146, 1420, 1615
HpyAV CCTTC 8 cut(s) 182, 301, 403, 572, 908, 1240, 1492, 1496
HpyCH4III ACNGT 3 cut(s) 118, 252, 993
HpyCH4IV ACGT 4 cut(s) 155, 533, 718, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 1401, 1589
HpyF3I CTNAG 4 cut(s) 199, 589, 929, 1120
HpySE526I ACGT 4 cut(s) 155, 533, 718, 797
Hsp92II CATG 7 cut(s) 56, 612, 1059, 1082, 1241, 1256, 1483
HspAI GCGC 1 cut(s) 1335
Ksp22I TGATCA 2 cut(s) 870, 1192
Kzo9I GATC 9 cut(s) 3, 102, 457, 692, 870, 1156, 1192, 1411, 1463
LmnI GCTCC 4 cut(s) 418, 472, 505, 1015
Lsp1109I GCAGC 2 cut(s) 310, 1567
LweI GCATC 3 cut(s) 205, 262, 644
MabI ACCWGGT 1 cut(s) 33
MaeI CTAG 6 cut(s) 131, 575, 696, 803, 1328, 1376
MaeII ACGT 4 cut(s) 155, 533, 718, 797
MaeIII GTNAC 5 cut(s) 44, 125, 151, 766, 798
MalI GATC 9 cut(s) 5, 104, 459, 694, 872, 1158, 1194, 1413, 1465
MboI GATC 9 cut(s) 3, 102, 457, 692, 870, 1156, 1192, 1411, 1463
MboII GAAGA 5 cut(s) 31, 392, 395, 491, 917
MfeI CAATTG 2 cut(s) 281, 954
MflI RGATCY 4 cut(s) 692, 1156, 1411, 1463
MhlI GDGCHC 3 cut(s) 116, 148, 530
MlsI TGGCCA 2 cut(s) 1404, 1457
MluCI AATT 9 cut(s) 23, 281, 291, 549, 663, 954, 998, 1107, 1319
MluNI TGGCCA 2 cut(s) 1404, 1457
MlyI GAGTC 3 cut(s) 344, 622, 1291
MmeI TCCRAC 6 cut(s) 234, 384, 1037, 1072, 1184, 1535
Mox20I TGGCCA 2 cut(s) 1404, 1457
Mph1103I ATGCAT 1 cut(s) 1481
MroXI GAANNNNTTC 1 cut(s) 387
MscI TGGCCA 2 cut(s) 1404, 1457
MseI TTAA 5 cut(s) 725, 857, 1110, 1140, 1149
MslI CAYNNNNRTG 1 cut(s) 1131
Msp20I TGGCCA 2 cut(s) 1404, 1457
MspI CCGG 2 cut(s) 1276, 1415
MspR9I CCNGG 4 cut(s) 35, 141, 543, 1296
MunI CAATTG 2 cut(s) 281, 954
Mva1269I GAATGC 3 cut(s) 325, 699, 1430
MvaI CCWGG 4 cut(s) 35, 141, 543, 1296
MwoI GCNNNNNNNGC 2 cut(s) 1401, 1589
NdeI CATATG 1 cut(s) 1487
NdeII GATC 9 cut(s) 3, 102, 457, 692, 870, 1156, 1192, 1411, 1463
NheI GCTAGC 1 cut(s) 1327
NlaIII CATG 7 cut(s) 56, 612, 1059, 1082, 1241, 1256, 1483
NlaIV GGNNCC 5 cut(s) 474, 507, 814, 1158, 1413
NmuCI GTSAC 1 cut(s) 766
NsiI ATGCAT 1 cut(s) 1481
NspI RCATGY 4 cut(s) 1059, 1082, 1256, 1483
NspV TTCGAA 1 cut(s) 1545
PaeI GCATGC 1 cut(s) 1483
PceI AGGCCT 1 cut(s) 171
PciI ACATGT 2 cut(s) 1078, 1252
PctI GAATGC 3 cut(s) 325, 699, 1430
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 7 cut(s) 56, 90, 388, 451, 650, 1346, 1509
PflMI CCANNNNNTGG 1 cut(s) 1278
PkrI GCNGC 2 cut(s) 325, 1582
PleI GAGTC 3 cut(s) 343, 621, 1290
PmaCI CACGTG 1 cut(s) 719
PmlI CACGTG 1 cut(s) 719
PpsI GAGTC 3 cut(s) 343, 621, 1290
Ppu21I YACGTR 1 cut(s) 719
PpuMI RGGWCCY 1 cut(s) 1292
PscI ACATGT 2 cut(s) 1078, 1252
Psp124BI GAGCTC 1 cut(s) 530
Psp1406I AACGTT 2 cut(s) 533, 797
Psp5II RGGWCCY 1 cut(s) 1292
Psp6I CCWGG 4 cut(s) 33, 139, 541, 1294
PspCI CACGTG 1 cut(s) 719
PspEI GGTNACC 1 cut(s) 125
PspGI CCWGG 4 cut(s) 33, 139, 541, 1294
PspN4I GGNNCC 5 cut(s) 474, 507, 814, 1158, 1413
PspPI GGNCC 3 cut(s) 669, 884, 1292
PspPPI RGGWCCY 1 cut(s) 1292
PstNI CAGNNNCTG 1 cut(s) 1295
PsuI RGATCY 4 cut(s) 692, 1156, 1411, 1463
RsaI GTAC 2 cut(s) 1072, 1451
RsaNI GTAC 2 cut(s) 1071, 1450
RseI CAYNNNNRTG 1 cut(s) 1131
SacI GAGCTC 1 cut(s) 530
SaqAI TTAA 5 cut(s) 725, 857, 1110, 1140, 1149
SatI GCNGC 2 cut(s) 324, 1581
Sau3AI GATC 9 cut(s) 3, 102, 457, 692, 870, 1156, 1192, 1411, 1463
Sau96I GGNCC 3 cut(s) 669, 884, 1292
ScaI AGTACT 1 cut(s) 1451
SchI GAGTC 3 cut(s) 344, 622, 1291
ScrFI CCNGG 4 cut(s) 35, 141, 543, 1296
SduI GDGCHC 3 cut(s) 116, 148, 530
SexAI ACCWGGT 1 cut(s) 33
SfaNI GCATC 3 cut(s) 205, 262, 644
SfcI CTRYAG 2 cut(s) 1287, 1390
SfuI TTCGAA 1 cut(s) 1545
SinI GGWCC 2 cut(s) 669, 1292
SmiMI CAYNNNNRTG 1 cut(s) 1131
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SphI GCATGC 1 cut(s) 1483
Sse9I AATT 9 cut(s) 23, 281, 291, 549, 663, 954, 998, 1107, 1319
SseBI AGGCCT 1 cut(s) 171
SsiI CCGC 4 cut(s) 98, 137, 194, 218
SspI AATATT 2 cut(s) 1232, 1542
SspMI CTAG 6 cut(s) 131, 575, 696, 803, 1328, 1376
SstI GAGCTC 1 cut(s) 530
StuI AGGCCT 1 cut(s) 171
StyD4I CCNGG 4 cut(s) 33, 139, 541, 1294
TaaI ACNGT 3 cut(s) 118, 252, 993
TaiI ACGT 4 cut(s) 158, 536, 721, 800
TaqI TCGA 6 cut(s) 93, 391, 449, 460, 661, 1545
TasI AATT 9 cut(s) 23, 281, 291, 549, 663, 954, 998, 1107, 1319
TatI WGTACW 1 cut(s) 1449
TfiI GAWTC 7 cut(s) 56, 90, 388, 451, 650, 1346, 1509
Tru1I TTAA 5 cut(s) 725, 857, 1110, 1140, 1149
Tru9I TTAA 5 cut(s) 725, 857, 1110, 1140, 1149
TscAI CASTG 3 cut(s) 685, 1465, 1531
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 2 cut(s) 323, 1580
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 5 cut(s) 69, 332, 723, 992, 1232
TspGWI ACGGA 2 cut(s) 32, 864
TspRI CASTG 3 cut(s) 685, 1465, 1531
Van91I CCANNNNNTGG 1 cut(s) 1278
VneI GTGCAC 1 cut(s) 144
VpaK11BI GGWCC 2 cut(s) 669, 1292
XapI RAATTY 3 cut(s) 549, 663, 998
XbaI TCTAGA 1 cut(s) 574
XceI RCATGY 4 cut(s) 1059, 1082, 1256, 1483
XcmI CCANNNNNNNNNTGG 1 cut(s) 1465
XmiI GTMKAC 1 cut(s) 1419
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 6 cut(s) 131, 575, 696, 803, 1328, 1376
ZrmI AGTACT 1 cut(s) 1451
Zsp2I ATGCAT 1 cut(s) 1481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.