FvH4_3g03070

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003805
Physical Location & Seq
Reverse (-)
1635670 .. 1636316
647 bp
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UTR
Exon/CDS
Intron
FvH4_3g03070.t1

Sequence Viewer

Length: 561 bp
ATGTTCGATAATGCCCAGAAGATGTTCGATGAAATGCCTGAGAGAAACTGCGCGCGCACCGTGCTGTCCTTCAACGCGCCTTTGGGCACGTGCGTGAACTCCAAGAGGTTTGATATTGCTGAGAAGATATTTAACACGATTTTGGATTGCTTGTATGGGAATCAGCGGTTTTCGGATGCTGAGAAGATGTGGGGTGTAATGGTGGAGAAGGATGTTGTTCCGGATATTAGAAGCTATAATGCCAAGAGGAGTAAAGGAGAGGCTGAATTGGTTGATGAGTTGAGGAGTAGTGGAGTGGAACCTGATAGTGGTCGTGCTCCGGATAAATGGACTTTCGAGACGCTTGTTCCCTTTGCTTGTGAGAAGGGTGATGTGGATTATGGTTTTGAGCTCTGCAAGGATATTTTCCAAAGGCGCCGTCTTGCTAGTGCGGCAATGTTGCAGCCTGTGGTGGATGGTCTGGTTAAGGCCTCTATGATTGAGCAGGCAAAGGAACTTGTCAAACTTGGGAACACTAATTCCTACAAACGTTTTAACTTGAAGTTGCCTTCAGACGAGTAG

Protein Analysis

187

Amino Acids

21.1

Weight (kDa)

5.28

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 43 - 80 9.7e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 414
AccII CGCG 3 cut(s) 53, 55, 77
AccIII TCCGGA 2 cut(s) 220, 319
AciI CCGC 2 cut(s) 166, 431
AclI AACGTT 1 cut(s) 529
AcuI CTGAAG 1 cut(s) 534
AcvI CACGTG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 415
AfiI CCNNNNNNNGG 1 cut(s) 308
AgsI TTSAA 2 cut(s) 73, 541
AleI CACNNNNGTG 1 cut(s) 92
AloI GAACNNNNNNTCC 2 cut(s) 503, 535
AluBI AGCT 2 cut(s) 234, 391
AluI AGCT 2 cut(s) 234, 391
Alw21I GWGCWC 2 cut(s) 319, 393
Alw26I GTCTC 1 cut(s) 332
Aor13HI TCCGGA 2 cut(s) 220, 319
AoxI GGCC 1 cut(s) 468
ApeKI GCWGC 1 cut(s) 442
ArsI GACNNNNNNTTYG 2 cut(s) 403, 435
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 5 cut(s) 53, 55, 57, 79, 417
AsuHPI GGTGA 1 cut(s) 380
BaeGI GKGCMC 1 cut(s) 89
BanI GGYRCC 1 cut(s) 414
BanII GRGCYC 1 cut(s) 393
BbrPI CACGTG 1 cut(s) 90
Bbv12I GWGCWC 2 cut(s) 319, 393
BbvI GCAGC 1 cut(s) 454
BccI CCATC 1 cut(s) 449
BceAI ACGGC 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 1 cut(s) 426
BfoI RGCGCY 1 cut(s) 418
BisI GCNGC 2 cut(s) 432, 443
BlsI GCNGC 2 cut(s) 433, 444
BmiI GGNNCC 2 cut(s) 300, 416
BmsI GCATC 1 cut(s) 166
BsaAI YACGTR 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 415
BsaWI WCCGGW 2 cut(s) 220, 319
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse3DI GCAATG 1 cut(s) 441
BseAI TCCGGA 2 cut(s) 220, 319
BseGI GGATG 3 cut(s) 181, 217, 460
BseLI CCNNNNNNNGG 1 cut(s) 308
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 3 cut(s) 30, 111, 171
BsePI GCGCGC 2 cut(s) 51, 53
BseRI GAGGAG 2 cut(s) 262, 298
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 1 cut(s) 454
Bsh1236I CGCG 3 cut(s) 53, 55, 77
BshFI GGCC 1 cut(s) 470
BshNI GGYRCC 1 cut(s) 414
BsiHKAI GWGCWC 2 cut(s) 319, 393
BsiSI CCGG 2 cut(s) 221, 320
BslI CCNNNNNNNGG 1 cut(s) 308
BsmAI GTCTC 1 cut(s) 332
BsmBI CGTCTC 1 cut(s) 332
BsnI GGCC 1 cut(s) 470
Bsp1286I GDGCHC 3 cut(s) 89, 319, 393
Bsp13I TCCGGA 2 cut(s) 220, 319
BspACI CCGC 2 cut(s) 166, 431
BspANI GGCC 1 cut(s) 470
BspCNI CTCAG 3 cut(s) 31, 112, 172
BspEI TCCGGA 2 cut(s) 220, 319
BspFNI CGCG 3 cut(s) 53, 55, 77
BspLI GGNNCC 2 cut(s) 300, 416
BspT107I GGYRCC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 441
BssHII GCGCGC 2 cut(s) 51, 53
BssNI GRCGYC 1 cut(s) 415
Bst4CI ACNGT 1 cut(s) 61
BstACI GRCGYC 1 cut(s) 415
BstBAI YACGTR 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 53, 55, 486
BstDEI CTNAG 3 cut(s) 39, 120, 180
BstF5I GGATG 3 cut(s) 181, 217, 460
BstFNI CGCG 3 cut(s) 53, 55, 77
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 5 cut(s) 53, 55, 57, 79, 417
BstMAI GTCTC 1 cut(s) 332
BstMWI GCNNNNNNNGC 2 cut(s) 61, 431
BstSLI GKGCMC 1 cut(s) 89
BstUI CGCG 3 cut(s) 53, 55, 77
BstV1I GCAGC 1 cut(s) 454
BsuRI GGCC 1 cut(s) 470
BtsCI GGATG 3 cut(s) 181, 217, 460
Cac8I GCNNGC 3 cut(s) 53, 55, 486
CfoI GCGC 5 cut(s) 53, 55, 57, 79, 417
CseI GACGC 1 cut(s) 349
CviJI RGCY 5 cut(s) 234, 263, 391, 445, 470
CviKI_1 RGCY 5 cut(s) 234, 263, 391, 445, 470
DdeI CTNAG 3 cut(s) 39, 120, 180
DinI GGCGCC 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 391
Eco147I AGGCCT 1 cut(s) 470
Eco24I GRGCYC 1 cut(s) 393
Eco53kI GAGCTC 1 cut(s) 391
Eco57I CTGAAG 1 cut(s) 534
Eco72I CACGTG 1 cut(s) 90
EcoICRI GAGCTC 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 393
EgeI GGCGCC 1 cut(s) 416
EheI GGCGCC 1 cut(s) 416
Esp3I CGTCTC 1 cut(s) 332
FaiI YATR 4 cut(s) 156, 237, 381, 476
Fnu4HI GCNGC 2 cut(s) 432, 443
FokI GGATG 3 cut(s) 188, 224, 467
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 2 cut(s) 432, 443
FspBI CTAG 1 cut(s) 426
GlaI GCGC 5 cut(s) 52, 54, 56, 78, 416
GluI GCNGC 2 cut(s) 432, 443
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 1 cut(s) 470
HapII CCGG 2 cut(s) 221, 320
HgaI GACGC 1 cut(s) 349
HhaI GCGC 5 cut(s) 53, 55, 57, 79, 417
Hin1I GRCGYC 1 cut(s) 415
Hin6I GCGC 5 cut(s) 51, 53, 55, 77, 415
HinP1I GCGC 5 cut(s) 51, 53, 55, 77, 415
HinfI GANTC 1 cut(s) 160
HpaII CCGG 2 cut(s) 221, 320
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 2 cut(s) 175, 553
Hpy188III TCNNGA 3 cut(s) 221, 320, 337
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 4 cut(s) 79, 202, 358, 558
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4IV ACGT 2 cut(s) 89, 529
HpyCH4V TGCA 2 cut(s) 396, 442
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 431
HpyF3I CTNAG 3 cut(s) 39, 120, 180
HpySE526I ACGT 2 cut(s) 89, 529
Hsp92I GRCGYC 1 cut(s) 415
HspAI GCGC 5 cut(s) 51, 53, 55, 77, 415
KasI GGCGCC 1 cut(s) 414
Kpn2I TCCGGA 2 cut(s) 220, 319
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 8 cut(s) 29, 51, 234, 315, 333, 446, 459, 470
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 1 cut(s) 166
MaeI CTAG 1 cut(s) 426
MaeII ACGT 2 cut(s) 89, 529
MboII GAAGA 3 cut(s) 31, 136, 196
MhlI GDGCHC 3 cut(s) 89, 319, 393
MluCI AATT 2 cut(s) 266, 517
Mly113I GGCGCC 1 cut(s) 415
MnlI CCTC 5 cut(s) 99, 240, 253, 276, 481
MroI TCCGGA 2 cut(s) 220, 319
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 3 cut(s) 132, 465, 534
MslI CAYNNNNRTG 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 2 cut(s) 221, 320
MvnI CGCG 3 cut(s) 53, 55, 77
MwoI GCNNNNNNNGC 2 cut(s) 61, 431
NarI GGCGCC 1 cut(s) 415
NlaIV GGNNCC 2 cut(s) 300, 416
OliI CACNNNNGTG 1 cut(s) 92
PauI GCGCGC 2 cut(s) 51, 53
PceI AGGCCT 1 cut(s) 470
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 160
PkrI GCNGC 2 cut(s) 433, 444
PluTI GGCGCC 1 cut(s) 418
PmaCI CACGTG 1 cut(s) 90
PmlI CACGTG 1 cut(s) 90
Ppu21I YACGTR 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 393
Psp1406I AACGTT 1 cut(s) 529
PspCI CACGTG 1 cut(s) 90
PspN4I GGNNCC 2 cut(s) 300, 416
PteI GCGCGC 2 cut(s) 51, 53
RseI CAYNNNNRTG 1 cut(s) 92
SacI GAGCTC 1 cut(s) 393
SaqAI TTAA 3 cut(s) 132, 465, 534
SatI GCNGC 2 cut(s) 432, 443
SduI GDGCHC 3 cut(s) 89, 319, 393
SetI ASST 6 cut(s) 92, 110, 236, 304, 393, 532
SfaNI GCATC 1 cut(s) 166
SfoI GGCGCC 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 92
Sse9I AATT 2 cut(s) 266, 517
SseBI AGGCCT 1 cut(s) 470
SsiI CCGC 2 cut(s) 166, 431
SspDI GGCGCC 1 cut(s) 414
SspMI CTAG 1 cut(s) 426
SstI GAGCTC 1 cut(s) 393
StuI AGGCCT 1 cut(s) 470
TaaI ACNGT 1 cut(s) 61
TaiI ACGT 2 cut(s) 92, 532
TaqI TCGA 3 cut(s) 6, 27, 336
TasI AATT 2 cut(s) 266, 517
TauI GCSGC 1 cut(s) 434
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 3 cut(s) 132, 465, 534
Tru9I TTAA 3 cut(s) 132, 465, 534
TseI GCWGC 1 cut(s) 442
TspDTI ATGAA 1 cut(s) 45
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.