MD05G1334700.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003805
Physical Location & Seq
Forward (+)
45773230 .. 45774372
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1334700.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGTCGTTCTCTCGCCTCCTCCAGCGCGCCTTCTGCACCGCCACCCAATCCAACGCCGCCACCACCAACAGCATTGGCGTCATCTCCACCAACCTCTACAAAGAGCGCAACCTGAAACGCCTCGTCGAGAAGTTTAAGAAGTCCTCCGAACTCTACCGCTTCCGTACCAAAACCCCCATTTACGAGGACGCCGTCCGCCGTCTCGCAGCCGCCAAACGTTTCAAATGGATCGAGGAGATCCTGGAGGACCAGAAGCAGTACCCGGACTTTTCCACGGAGGGATTCGCTGTTCGACTGGTTACGCTTTACGGGAAAGCTGGCATGTTCGAAAATGCCCAGAAGGTGTTCGACGAAATGCCTGAGAAAAGCTGTGAGCGCACCATCTTGTCCTTTAATGCCCTCTTGGGTGCTTGTGTTAACTCCAAGAAATTCGACAAGGTTGCTGAGGTTTTTAGAGGGGTCTCGCAGAAGTTGTCAATTGAGCCGGATGTGGTGTCGTATAATACTGTGGTCAAGGCGTTTTGTGAAATGGGTTCGTTGGAATCGGCGGTTTCAATGCTTGTTGAGATGGAGAATAAGGGTGTTGAGCCGGATGTGATCACATTCAACACGATCATGAATGGGTTGTATGCGAATAATCAGTTTTTGGCAGCCGAAAAAATATGGAGTCGAATGGGGGAGAAGAATGTTGTGCCTGATGTTAGGAGCTACAATGCCAGGTTGCTGGGATTGGCTTCGGAGAAGAAAACTAACGAGATGGTTGAGTTATTCGAGGAAATGAGGAACAAGGAAGTGAAACCTGATGTGTTTAGCTTCAATGCCTTGATCAAGGGATTTGTTAATGAGGGGAAATTGGAAGAAGCTAAACAGTGGTACCGAGAACTGGTGAAGAGTAGTTGTGCTCTGCAGAAATGGACTTTCCAGACGCTTGTTCCCTTTGTTTGTGAGAAGGGTGACTTGGACTTTGCCTTTGAACTCGGCGAGGAAATTTTCAAAAGGCGCCTCCTTATCGATGTGGAGCTATTGCAGGTTGTGGTGGACGGCCTGGCTAAGGCTTCCAGAATCAATGAAGCAAAGAAGATTGTGAAGCTTGGGAACACAAATACTTACCGGCGTTACAATTTGCAGTTGCCTTCGGACTAA

Protein Analysis

381

Amino Acids

43.48

Weight (kDa)

8.79

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 99 - 140 7.8e-06 PPR repeat family
PPR_3 PF13812 99 - 140 4.1e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 157 - 207 3.8e-10 Pentatricopeptide repeat domain
TPR_24 PF23276 157 - 294 3.5e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 160 - 191 2.1e-09 PPR repeat
PPR_2 PF13041 162 - 209 6.8e-16 PPR repeat family
PPR PF01535 165 - 195 3.7e-06 PPR repeat
PPR_3 PF13812 186 - 239 1.7e-08 Pentatricopeptide repeat domain
PPR_long PF17177 189 - 297 3.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 197 - 245 6.4e-11 PPR repeat family
PPR_3 PF13812 221 - 281 4.1e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 232 - 281 7.4e-12 PPR repeat family
PPR_1 PF12854 263 - 290 6.5e-06 PPR repeat
PPR_2 PF13041 267 - 311 6.3e-09 PPR repeat family
PPR PF01535 270 - 300 8.1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1018
Acc65I GGTACC 1 cut(s) 873
AccB1I GGYRCC 2 cut(s) 873, 999
AccII CGCG 1 cut(s) 27
AciI CCGC 6 cut(s) 39, 57, 157, 196, 210, 548
AclI AACGTT 1 cut(s) 217
AclWI GGATC 2 cut(s) 232, 236
AcsI RAATTY 2 cut(s) 428, 987
AcyI GRCGYC 3 cut(s) 78, 189, 1000
AfaI GTAC 3 cut(s) 166, 260, 875
AfiI CCNNNNNNNGG 2 cut(s) 883, 1051
AgsI TTSAA 6 cut(s) 223, 555, 607, 817, 974, 994
AjnI CCWGG 3 cut(s) 240, 716, 1044
AjuI GAANNNNNNNTTGG 2 cut(s) 941, 973
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
AluBI AGCT 7 cut(s) 317, 369, 708, 813, 863, 1021, 1090
AluI AGCT 7 cut(s) 317, 369, 708, 813, 863, 1021, 1090
Alw21I GWGCWC 1 cut(s) 904
Alw26I GTCTC 2 cut(s) 206, 466
AlwI GGATC 2 cut(s) 232, 236
AoxI GGCC 1 cut(s) 1042
ApeKI GCWGC 2 cut(s) 206, 650
ApoI RAATTY 2 cut(s) 428, 987
ArsI GACNNNNNNTTYG 4 cut(s) 947, 953, 979, 985
Asp700I GAANNNNTTC 1 cut(s) 344
Asp718I GGTACC 1 cut(s) 873
AspLEI GCGC 5 cut(s) 27, 29, 108, 378, 1002
AspS9I GGNCC 1 cut(s) 247
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 898, 965
AsuII TTCGAA 1 cut(s) 327
AvaII GGWCC 1 cut(s) 247
BaeI ACNNNNGTAYC 2 cut(s) 857, 890
BanI GGYRCC 2 cut(s) 873, 999
Bbv12I GWGCWC 1 cut(s) 904
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 2 cut(s) 218, 662
BccI CCATC 3 cut(s) 389, 562, 751
BceAI ACGGC 3 cut(s) 176, 183, 1057
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 3 cut(s) 242, 718, 1046
BclI TGATCA 2 cut(s) 597, 825
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 2 cut(s) 206, 466
BfmI CTRYAG 1 cut(s) 905
BfoI RGCGCY 1 cut(s) 1003
BfuAI ACCTGC 1 cut(s) 1018
BisI GCNGC 4 cut(s) 57, 207, 210, 651
BlsI GCNGC 4 cut(s) 58, 208, 211, 652
Bme1390I CCNGG 4 cut(s) 242, 263, 718, 1046
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 2 cut(s) 875, 1001
BmrFI CCNGG 4 cut(s) 242, 263, 718, 1046
BpmI CTGGAG 2 cut(s) 5, 263
Bpu10I CCTNAGC 2 cut(s) 444, 1050
Bpu14I TTCGAA 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 263
Bsa29I ATCGAT 1 cut(s) 1011
BsaHI GRCGYC 3 cut(s) 78, 189, 1000
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 1 cut(s) 273
Bsc4I CCNNNNNNNGG 2 cut(s) 883, 1051
Bse118I RCCGGY 1 cut(s) 1110
Bse1I ACTGG 2 cut(s) 300, 888
BseBI CCWGG 3 cut(s) 242, 718, 1046
BseCI ATCGAT 1 cut(s) 1011
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 2 cut(s) 493, 598
BseLI CCNNNNNNNGG 2 cut(s) 883, 1051
BseMII CTCAG 2 cut(s) 351, 435
BseNI ACTGG 2 cut(s) 300, 888
BsePI GCGCGC 1 cut(s) 25
BseRI GAGGAG 2 cut(s) 8, 248
BseXI GCAGC 2 cut(s) 218, 662
BseYI CCCAGC 1 cut(s) 724
BsgI GTGCAG 1 cut(s) 19
Bsh1236I CGCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 1044
BshNI GGYRCC 2 cut(s) 873, 999
BshVI ATCGAT 1 cut(s) 1011
BsiHKAI GWGCWC 1 cut(s) 904
BsiSI CCGG 4 cut(s) 263, 485, 590, 1111
BslI CCNNNNNNNGG 2 cut(s) 883, 1051
BsmAI GTCTC 2 cut(s) 206, 466
BsmBI CGTCTC 1 cut(s) 206
BsnI GGCC 1 cut(s) 1044
Bso31I GGTCTC 1 cut(s) 466
Bsp119I TTCGAA 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 904
Bsp143I GATC 5 cut(s) 228, 237, 597, 612, 825
BspACI CCGC 6 cut(s) 39, 57, 157, 196, 210, 548
BspANI GGCC 1 cut(s) 1044
BspCNI CTCAG 2 cut(s) 352, 436
BspDI ATCGAT 1 cut(s) 1011
BspFNI CGCG 1 cut(s) 27
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 2 cut(s) 875, 1001
BspMAI CTGCAG 1 cut(s) 909
BspMI ACCTGC 1 cut(s) 1018
BspPI GGATC 2 cut(s) 232, 236
BspT104I TTCGAA 1 cut(s) 327
BspT107I GGYRCC 2 cut(s) 873, 999
BspTNI GGTCTC 1 cut(s) 466
BsrFI RCCGGY 1 cut(s) 1110
BsrI ACTGG 2 cut(s) 300, 888
BssAI RCCGGY 1 cut(s) 1110
BssECI CCNNGG 1 cut(s) 273
BssHII GCGCGC 1 cut(s) 25
BssMI GATC 5 cut(s) 228, 237, 597, 612, 825
BssNI GRCGYC 3 cut(s) 78, 189, 1000
Bst2UI CCWGG 3 cut(s) 242, 718, 1046
Bst4CI ACNGT 2 cut(s) 508, 870
Bst6I CTCTTC 1 cut(s) 884
BstACI GRCGYC 3 cut(s) 78, 189, 1000
BstBI TTCGAA 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 27, 319
BstDEI CTNAG 3 cut(s) 360, 444, 1050
BstDSI CCRYGG 1 cut(s) 273
BstENI CCTNNNNNAGG 1 cut(s) 1049
BstF5I GGATG 2 cut(s) 493, 598
BstFNI CGCG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 1003
BstHHI GCGC 5 cut(s) 27, 29, 108, 378, 1002
BstKTI GATC 5 cut(s) 231, 240, 600, 615, 828
BstMAI GTCTC 2 cut(s) 206, 466
BstMBI GATC 5 cut(s) 228, 237, 597, 612, 825
BstMWI GCNNNNNNNGC 2 cut(s) 33, 375
BstNI CCWGG 3 cut(s) 242, 718, 1046
BstNSI RCATGY 1 cut(s) 325
BstSCI CCNGG 4 cut(s) 240, 261, 716, 1044
BstSFI CTRYAG 1 cut(s) 905
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 2 cut(s) 218, 662
BstX2I RGATCY 1 cut(s) 237
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 1 cut(s) 237
Bsu15I ATCGAT 1 cut(s) 1011
BsuRI GGCC 1 cut(s) 1044
BsuTUI ATCGAT 1 cut(s) 1011
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 493, 598
BtsIMutI CAGTG 1 cut(s) 875
BveI ACCTGC 1 cut(s) 1018
Cac8I GCNNGC 2 cut(s) 27, 319
CciI TCATGA 1 cut(s) 615
CfoI GCGC 5 cut(s) 27, 29, 108, 378, 1002
Cfr10I RCCGGY 1 cut(s) 1110
Cfr13I GGNCC 1 cut(s) 247
ClaI ATCGAT 1 cut(s) 1011
CseI GACGC 3 cut(s) 67, 197, 934
Csp6I GTAC 3 cut(s) 165, 259, 874
CviAII CATG 2 cut(s) 322, 616
CviQI GTAC 3 cut(s) 165, 259, 874
DdeI CTNAG 3 cut(s) 360, 444, 1050
DinI GGCGCC 1 cut(s) 1001
DpnI GATC 5 cut(s) 230, 239, 599, 614, 827
DpnII GATC 5 cut(s) 228, 237, 597, 612, 825
Eam1104I CTCTTC 1 cut(s) 884
EarI CTCTTC 1 cut(s) 884
EciI GGCGGA 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 247
EcoNI CCTNNNNNAGG 1 cut(s) 1049
EcoRII CCWGG 3 cut(s) 240, 716, 1044
EgeI GGCGCC 1 cut(s) 1001
EheI GGCGCC 1 cut(s) 1001
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 2 cut(s) 325, 619
FaiI YATR 5 cut(s) 323, 501, 617, 630, 664
FalI AAGNNNNNCTT 2 cut(s) 941, 973
FatI CATG 2 cut(s) 321, 615
FbaI TGATCA 2 cut(s) 597, 825
Fnu4HI GCNGC 4 cut(s) 57, 207, 210, 651
FokI GGATG 2 cut(s) 500, 605
Fsp4HI GCNGC 4 cut(s) 57, 207, 210, 651
GlaI GCGC 5 cut(s) 26, 28, 107, 377, 1001
GluI GCNGC 4 cut(s) 57, 207, 210, 651
GsaI CCCAGC 1 cut(s) 728
GsuI CTGGAG 2 cut(s) 5, 263
HaeII RGCGCY 1 cut(s) 1003
HaeIII GGCC 1 cut(s) 1044
HapII CCGG 4 cut(s) 263, 485, 590, 1111
HgaI GACGC 3 cut(s) 67, 197, 934
HhaI GCGC 5 cut(s) 27, 29, 108, 378, 1002
Hin1I GRCGYC 3 cut(s) 78, 189, 1000
Hin1II CATG 2 cut(s) 325, 619
Hin6I GCGC 5 cut(s) 25, 27, 106, 376, 1000
HinP1I GCGC 5 cut(s) 25, 27, 106, 376, 1000
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 1088
HinfI GANTC 4 cut(s) 282, 542, 667, 1062
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 4 cut(s) 263, 485, 590, 1111
HphI GGTGA 2 cut(s) 898, 965
Hpy166II GTNNAC 2 cut(s) 418, 1039
Hpy188I TCNGA 3 cut(s) 148, 739, 1138
Hpy188III TCNNGA 4 cut(s) 127, 616, 922, 1059
Hpy8I GTNNAC 2 cut(s) 418, 1039
Hpy99I CGWCG 2 cut(s) 128, 353
HpyAV CCTTC 4 cut(s) 40, 334, 943, 1143
HpyCH4III ACNGT 2 cut(s) 508, 870
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 4 cut(s) 36, 907, 1027, 1126
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 375
HpyF3I CTNAG 3 cut(s) 360, 444, 1050
HpySE526I ACGT 1 cut(s) 217
Hsp92I GRCGYC 3 cut(s) 78, 189, 1000
Hsp92II CATG 2 cut(s) 325, 619
HspAI GCGC 5 cut(s) 25, 27, 106, 376, 1000
KasI GGCGCC 1 cut(s) 999
KpnI GGTACC 1 cut(s) 877
Ksp22I TGATCA 2 cut(s) 597, 825
KspAI GTTAAC 1 cut(s) 418
Kzo9I GATC 5 cut(s) 228, 237, 597, 612, 825
LmnI GCTCC 2 cut(s) 705, 1018
Lsp1109I GCAGC 2 cut(s) 218, 662
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 3 cut(s) 298, 953, 1115
MalI GATC 5 cut(s) 230, 239, 599, 614, 827
MboI GATC 5 cut(s) 228, 237, 597, 612, 825
MboII GAAGA 5 cut(s) 694, 754, 869, 901, 1090
MfeI CAATTG 1 cut(s) 477
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 1 cut(s) 904
MluCI AATT 5 cut(s) 428, 477, 851, 987, 1120
Mly113I GGCGCC 1 cut(s) 1000
MlyI GAGTC 1 cut(s) 676
MmeI TCCRAC 2 cut(s) 75, 519
MroXI GAANNNNTTC 1 cut(s) 344
MseI TTAA 4 cut(s) 135, 393, 417, 840
MslI CAYNNNNRTG 1 cut(s) 614
MspI CCGG 4 cut(s) 263, 485, 590, 1111
MspR9I CCNGG 4 cut(s) 242, 263, 718, 1046
MunI CAATTG 1 cut(s) 477
MvaI CCWGG 3 cut(s) 242, 718, 1046
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 33, 375
NarI GGCGCC 1 cut(s) 1000
NciI CCSGG 1 cut(s) 263
NdeII GATC 5 cut(s) 228, 237, 597, 612, 825
NlaIII CATG 2 cut(s) 325, 619
NlaIV GGNNCC 2 cut(s) 875, 1001
NmeAIII GCCGAG 1 cut(s) 957
NmuCI GTSAC 1 cut(s) 953
NspI RCATGY 1 cut(s) 325
NspV TTCGAA 1 cut(s) 327
PagI TCATGA 1 cut(s) 615
PauI GCGCGC 1 cut(s) 25
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 3 cut(s) 282, 542, 1062
PflFI GACNNNGTC 1 cut(s) 191
PfoI TCCNGGA 1 cut(s) 240
PkrI GCNGC 4 cut(s) 58, 208, 211, 652
PleI GAGTC 1 cut(s) 675
PluTI GGCGCC 1 cut(s) 1003
PpsI GAGTC 1 cut(s) 675
Psp1406I AACGTT 1 cut(s) 217
Psp6I CCWGG 3 cut(s) 240, 716, 1044
PspFI CCCAGC 1 cut(s) 724
PspGI CCWGG 3 cut(s) 240, 716, 1044
PspN4I GGNNCC 2 cut(s) 875, 1001
PspPI GGNCC 1 cut(s) 247
PstI CTGCAG 1 cut(s) 909
PsuI RGATCY 1 cut(s) 237
PsyI GACNNNGTC 1 cut(s) 191
PteI GCGCGC 1 cut(s) 25
RsaI GTAC 3 cut(s) 166, 260, 875
RsaNI GTAC 3 cut(s) 165, 259, 874
RseI CAYNNNNRTG 1 cut(s) 614
SaqAI TTAA 4 cut(s) 135, 393, 417, 840
SatI GCNGC 4 cut(s) 57, 207, 210, 651
Sau3AI GATC 5 cut(s) 228, 237, 597, 612, 825
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 1 cut(s) 676
ScrFI CCNGG 4 cut(s) 242, 263, 718, 1046
SduI GDGCHC 1 cut(s) 904
SfcI CTRYAG 1 cut(s) 905
SfoI GGCGCC 1 cut(s) 1001
SfuI TTCGAA 1 cut(s) 327
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 614
Sse9I AATT 5 cut(s) 428, 477, 851, 987, 1120
SsiI CCGC 6 cut(s) 39, 57, 157, 196, 210, 548
SspDI GGCGCC 1 cut(s) 999
StyD4I CCNGG 4 cut(s) 240, 261, 716, 1044
TaaI ACNGT 2 cut(s) 508, 870
TaiI ACGT 1 cut(s) 220
TaqI TCGA 9 cut(s) 126, 231, 292, 327, 348, 432, 670, 771, 1011
TasI AATT 5 cut(s) 428, 477, 851, 987, 1120
TauI GCSGC 2 cut(s) 59, 212
TfiI GAWTC 3 cut(s) 282, 542, 1062
Tru1I TTAA 4 cut(s) 135, 393, 417, 840
Tru9I TTAA 4 cut(s) 135, 393, 417, 840
TscAI CASTG 1 cut(s) 875
TseFI GTSAC 1 cut(s) 953
TseI GCWGC 2 cut(s) 206, 650
Tsp45I GTSAC 1 cut(s) 953
TspDTI ATGAA 2 cut(s) 632, 1083
TspGWI ACGGA 2 cut(s) 152, 290
TspRI CASTG 1 cut(s) 875
Tth111I GACNNNGTC 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 1049
XapI RAATTY 2 cut(s) 428, 987
XceI RCATGY 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.