RLG00000031259

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003805
Physical Location & Seq
Reverse (-)
3452952 .. 3454100
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031259

Sequence Viewer

Length: 1149 bp
ATGTCGTTTTGTCGCCTCCTCCGCCGTGCATTTGCCACCGCCGCCACCCAGCCCAAACCTACAACCGCCAAGAGCATCAGAGCCATCTCCGCCGACCTCGACAGAGAGCGCAACCTCAAACGCCTCGTCAAGAAGTTCAAAGAGTCCTCAGAGCTCTACCGCTTCCGAACCAAAACCCCCATTTACGAAGAGACCGTTCGCCGCCTCACCAACGCCAAGCGCTTCCGTGCGATCGAAGAAATTTTAGAGGACCAGAAGAAGTACCCGGATATCTCGGACGAGGGTTTCGCCGTCCGAATCCTTTCCCTTTATGGAAAGGCTGGCATGTTTGATAATGCCCAGAAGGTGTTCGACGAAATGCCTGAGAGAAACTGCGGCCGCACCGTGTTGTCTTTCAATGCCCTCCTGGGGGCCTGCGTGAACTCCAAGAAGTTTGATATGGTTGAGAAGATTTTCGGGGAGGTACCGGAGAAGTTGTCGATTGAGCCGGATTTGGTGTCGTACAATACTGTGATCAAGGCATTTTGCAAGATGGGTTCGTTTGACTCTGCGGTTTTGGTGATTGATGTGATGGAGAAGAAGGGTGTGAAGCCGGATGTAATCACGTTTAATACGATTTTGGATTGCTTGTATGGGAATAAGCGGTTTTTGGATGCTGAGAAGATATGGGGTCAAATGGTGGAGAAGAATGTCGTTCCTAATATTAGGAGCTACAATGCCAAATTGCTGGGGTTGGCTTTGGAGAAGAGGAGTGAAGAAGGGGCTCAGTTAGTTGAAGAGTTGAGGAATATTGGAGTGAAACCTGATGTGTTCACCTTCAATGCCTTGATTAAGGGCTTTGTGAATGAGGGGAACTTGGATGAAGCTAAGCGGTGGTATGGTGAAATAGAGAAGAGTAGTTGTGCTCCAGATAAATGGACTTTCGAGACGCTTGTTCCCTTTGTTTGTGAGAAGGGTGATGTTGAGTTTGCTTTTGAGCTCTGCAAGGACATTTTCCAAAGGCGCCGCCTTGTGAGTGCGGCACTGTTGCAGCCTGTGGTGGATGGACTGGTCAAGGCCTCCATGATTGAGGAGGCAAAGGAACTTGTCAAACTTGGGAACACTAATAACTACAGACGTTTTAATCTGAAGTTGACTTCAGACAAATAG

Protein Analysis

383

Amino Acids

43.59

Weight (kDa)

8.98

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 100 - 141 4.7e-06 PPR repeat family
PPR_long PF17177 110 - 200 3.5e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 115 - 177 6.6e-09 Pentatricopeptide repeat domain
PPR_3 PF13812 158 - 209 2.2e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 161 - 192 6.1e-09 PPR repeat
PPR_2 PF13041 163 - 210 4.7e-15 PPR repeat family
PPR PF01535 166 - 196 1e-06 PPR repeat
PPR_3 PF13812 187 - 240 7.5e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 198 - 245 7.2e-10 PPR repeat family
PPR_2 PF13041 233 - 282 2e-07 PPR repeat family
PPR_3 PF13812 257 - 311 2.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 265 - 291 8.5e-08 PPR repeat
PPR_2 PF13041 268 - 312 2.3e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 463
AccB1I GGYRCC 2 cut(s) 463, 1002
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 1 cut(s) 240
AcuI CTGAAG 2 cut(s) 1122, 1148
AcyI GRCGYC 1 cut(s) 1003
AfaI GTAC 3 cut(s) 263, 465, 503
AfeI AGCGCT 1 cut(s) 221
AfiI CCNNNNNNNGG 2 cut(s) 408, 409
AgsI TTSAA 4 cut(s) 139, 397, 776, 820
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 4 cut(s) 154, 711, 866, 979
AluI AGCT 4 cut(s) 154, 711, 866, 979
Alw21I GWGCWC 3 cut(s) 156, 907, 981
Alw26I GTCTC 2 cut(s) 185, 920
Aor51HI AGCGCT 1 cut(s) 221
AoxI GGCC 3 cut(s) 376, 411, 1056
ApeKI GCWGC 1 cut(s) 1030
ApoI RAATTY 1 cut(s) 240
ArsI GACNNNNNNTTYG 4 cut(s) 111, 143, 269, 301
Asp700I GAANNNNTTC 3 cut(s) 301, 347, 452
Asp718I GGTACC 1 cut(s) 463
AspLEI GCGC 3 cut(s) 111, 222, 1005
AspS9I GGNCC 2 cut(s) 250, 411
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 5 cut(s) 199, 571, 805, 893, 968
AvaII GGWCC 1 cut(s) 250
BanI GGYRCC 2 cut(s) 463, 1002
BanII GRGCYC 3 cut(s) 156, 766, 981
Bbv12I GWGCWC 3 cut(s) 156, 907, 981
BbvI GCAGC 1 cut(s) 1042
BccI CCATC 4 cut(s) 92, 526, 565, 1037
BceAI ACGGC 2 cut(s) 9, 275
BciT130I CCWGG 1 cut(s) 407
BclI TGATCA 1 cut(s) 513
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 2 cut(s) 185, 920
BfmI CTRYAG 1 cut(s) 1111
BfoI RGCGCY 2 cut(s) 223, 1006
BisI GCNGC 7 cut(s) 42, 202, 376, 379, 1006, 1020, 1031
BlpI GCTNAGC 1 cut(s) 867
BlsI GCNGC 7 cut(s) 43, 203, 377, 380, 1007, 1021, 1032
Bme1390I CCNGG 2 cut(s) 266, 407
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 250, 411
BmiI GGNNCC 3 cut(s) 412, 465, 1004
BmrFI CCNGG 2 cut(s) 266, 407
BmsI GCATC 2 cut(s) 84, 643
BpmI CTGGAG 1 cut(s) 891
Bpu1102I GCTNAGC 1 cut(s) 867
BpuMI CCSGG 1 cut(s) 266
BsaHI GRCGYC 1 cut(s) 1003
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 406
BsaWI WCCGGW 1 cut(s) 466
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 408, 409
Bse1I ACTGG 1 cut(s) 1053
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 1 cut(s) 406
BseGI GGATG 4 cut(s) 601, 658, 865, 1048
BseLI CCNNNNNNNGG 2 cut(s) 408, 409
BseMII CTCAG 4 cut(s) 162, 354, 648, 779
BseNI ACTGG 1 cut(s) 1053
BseRI GAGGAG 3 cut(s) 8, 763, 1085
BseX3I CGGCCG 1 cut(s) 376
BseXI GCAGC 1 cut(s) 1042
BseYI CCCAGC 2 cut(s) 48, 727
Bsh1285I CGRYCG 2 cut(s) 234, 379
BshFI GGCC 3 cut(s) 378, 413, 1058
BshNI GGYRCC 2 cut(s) 463, 1002
BsiEI CGRYCG 2 cut(s) 234, 379
BsiHKAI GWGCWC 3 cut(s) 156, 907, 981
BsiSI CCGG 4 cut(s) 266, 467, 488, 593
BslI CCNNNNNNNGG 2 cut(s) 408, 409
BsmAI GTCTC 2 cut(s) 185, 920
BsmBI CGTCTC 1 cut(s) 920
BsnI GGCC 3 cut(s) 378, 413, 1058
Bso31I GGTCTC 1 cut(s) 185
Bsp1286I GDGCHC 4 cut(s) 156, 766, 907, 981
Bsp143I GATC 2 cut(s) 231, 513
Bsp1720I GCTNAGC 1 cut(s) 867
BspANI GGCC 3 cut(s) 378, 413, 1058
BspCNI CTCAG 4 cut(s) 161, 355, 649, 778
BspLI GGNNCC 3 cut(s) 412, 465, 1004
BspT107I GGYRCC 2 cut(s) 463, 1002
BspTNI GGTCTC 1 cut(s) 185
BsrI ACTGG 1 cut(s) 1053
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 2 cut(s) 231, 513
BssNI GRCGYC 1 cut(s) 1003
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 4 cut(s) 196, 385, 511, 1026
Bst6I CTCTTC 4 cut(s) 183, 740, 771, 887
BstACI GRCGYC 1 cut(s) 1003
BstC8I GCNNGC 2 cut(s) 322, 415
BstDEI CTNAG 5 cut(s) 148, 363, 657, 765, 867
BstF5I GGATG 4 cut(s) 601, 658, 865, 1048
BstH2I RGCGCY 2 cut(s) 223, 1006
BstHHI GCGC 3 cut(s) 111, 222, 1005
BstKTI GATC 2 cut(s) 234, 516
BstMAI GTCTC 2 cut(s) 185, 920
BstMBI GATC 2 cut(s) 231, 513
BstMCI CGRYCG 2 cut(s) 234, 379
BstMWI GCNNNNNNNGC 3 cut(s) 21, 41, 89
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 2 cut(s) 264, 405
BstSFI CTRYAG 1 cut(s) 1111
BstV1I GCAGC 1 cut(s) 1042
BstXI CCANNNNNNTGG 2 cut(s) 727, 915
BstZI CGGCCG 1 cut(s) 376
BsuRI GGCC 3 cut(s) 378, 413, 1058
BtsCI GGATG 4 cut(s) 601, 658, 865, 1048
BtsIMutI CAGTG 1 cut(s) 1022
Cac8I GCNNGC 2 cut(s) 322, 415
CciNI GCGGCCGC 1 cut(s) 376
CfoI GCGC 3 cut(s) 111, 222, 1005
Cfr13I GGNCC 2 cut(s) 250, 411
CseI GACGC 1 cut(s) 937
Csp6I GTAC 3 cut(s) 262, 464, 502
CviAII CATG 2 cut(s) 325, 1063
CviQI GTAC 3 cut(s) 262, 464, 502
DdeI CTNAG 5 cut(s) 148, 363, 657, 765, 867
DinI GGCGCC 1 cut(s) 1004
DpnI GATC 2 cut(s) 233, 515
DpnII GATC 2 cut(s) 231, 513
EaeI YGGCCR 1 cut(s) 376
EagI CGGCCG 1 cut(s) 376
Eam1104I CTCTTC 4 cut(s) 183, 740, 771, 887
EarI CTCTTC 4 cut(s) 183, 740, 771, 887
EciI GGCGGA 2 cut(s) 11, 79
Ecl136II GAGCTC 2 cut(s) 154, 979
EclXI CGGCCG 1 cut(s) 376
Eco147I AGGCCT 1 cut(s) 1058
Eco24I GRGCYC 3 cut(s) 156, 766, 981
Eco31I GGTCTC 1 cut(s) 185
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 250
Eco47III AGCGCT 1 cut(s) 221
Eco52I CGGCCG 1 cut(s) 376
Eco53kI GAGCTC 2 cut(s) 154, 979
Eco57I CTGAAG 2 cut(s) 1122, 1148
EcoICRI GAGCTC 2 cut(s) 154, 979
EcoO109I RGGNCCY 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 405
EcoRV GATATC 1 cut(s) 271
EcoT38I GRGCYC 3 cut(s) 156, 766, 981
EgeI GGCGCC 1 cut(s) 1004
EheI GGCGCC 1 cut(s) 1004
Esp3I CGTCTC 1 cut(s) 920
FaeI CATG 2 cut(s) 328, 1066
FaiI YATR 7 cut(s) 312, 326, 440, 633, 667, 879, 1064
FatI CATG 2 cut(s) 324, 1062
FbaI TGATCA 1 cut(s) 513
Fnu4HI GCNGC 7 cut(s) 42, 202, 376, 379, 1006, 1020, 1031
FokI GGATG 4 cut(s) 608, 665, 872, 1055
FriOI GRGCYC 3 cut(s) 156, 766, 981
Fsp4HI GCNGC 7 cut(s) 42, 202, 376, 379, 1006, 1020, 1031
GlaI GCGC 3 cut(s) 110, 221, 1004
GluI GCNGC 7 cut(s) 42, 202, 376, 379, 1006, 1020, 1031
GsaI CCCAGC 2 cut(s) 52, 731
GsuI CTGGAG 1 cut(s) 891
HaeII RGCGCY 2 cut(s) 223, 1006
HaeIII GGCC 3 cut(s) 378, 413, 1058
HapII CCGG 4 cut(s) 266, 467, 488, 593
HgaI GACGC 1 cut(s) 937
HhaI GCGC 3 cut(s) 111, 222, 1005
Hin1I GRCGYC 1 cut(s) 1003
Hin1II CATG 2 cut(s) 328, 1066
Hin6I GCGC 3 cut(s) 109, 220, 1003
HinP1I GCGC 3 cut(s) 109, 220, 1003
HincII GTYRAC 1 cut(s) 1134
HindII GTYRAC 1 cut(s) 1134
HinfI GANTC 3 cut(s) 143, 297, 545
HpaII CCGG 4 cut(s) 266, 467, 488, 593
HphI GGTGA 5 cut(s) 199, 571, 805, 893, 968
Hpy166II GTNNAC 3 cut(s) 421, 813, 1134
Hpy188I TCNGA 7 cut(s) 80, 151, 167, 277, 296, 1128, 1141
Hpy188III TCNNGA 3 cut(s) 130, 908, 925
Hpy8I GTNNAC 3 cut(s) 421, 813, 1134
Hpy99I CGWCG 1 cut(s) 356
HpyAV CCTTC 5 cut(s) 337, 574, 752, 826, 946
HpyCH4III ACNGT 4 cut(s) 196, 385, 511, 1026
HpyCH4IV ACGT 2 cut(s) 605, 1117
HpyCH4V TGCA 4 cut(s) 29, 528, 984, 1030
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 41, 89
HpyF3I CTNAG 5 cut(s) 148, 363, 657, 765, 867
HpySE526I ACGT 2 cut(s) 605, 1117
Hsp92I GRCGYC 1 cut(s) 1003
Hsp92II CATG 2 cut(s) 328, 1066
HspAI GCGC 3 cut(s) 109, 220, 1003
KasI GGCGCC 1 cut(s) 1002
KpnI GGTACC 1 cut(s) 467
Ksp22I TGATCA 1 cut(s) 513
Kzo9I GATC 2 cut(s) 231, 513
LmnI GCTCC 2 cut(s) 708, 910
Lsp1109I GCAGC 1 cut(s) 1042
LweI GCATC 2 cut(s) 84, 643
MaeII ACGT 2 cut(s) 605, 1117
MalI GATC 2 cut(s) 233, 515
MboI GATC 2 cut(s) 231, 513
MhlI GDGCHC 4 cut(s) 156, 766, 907, 981
MluCI AATT 2 cut(s) 240, 722
Mly113I GGCGCC 1 cut(s) 1003
MlyI GAGTC 2 cut(s) 152, 539
MroXI GAANNNNTTC 3 cut(s) 301, 347, 452
MseI TTAA 3 cut(s) 609, 831, 1122
MspI CCGG 4 cut(s) 266, 467, 488, 593
MspR9I CCNGG 2 cut(s) 266, 407
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 3 cut(s) 21, 41, 89
NarI GGCGCC 1 cut(s) 1003
NciI CCSGG 1 cut(s) 266
NdeII GATC 2 cut(s) 231, 513
NlaIII CATG 2 cut(s) 328, 1066
NlaIV GGNNCC 3 cut(s) 412, 465, 1004
NotI GCGGCCGC 1 cut(s) 376
NspI RCATGY 1 cut(s) 328
PceI AGGCCT 1 cut(s) 1058
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 611
PdmI GAANNNNTTC 3 cut(s) 301, 347, 452
PfeI GAWTC 1 cut(s) 297
PkrI GCNGC 7 cut(s) 43, 203, 377, 380, 1007, 1021, 1032
Ple19I CGATCG 1 cut(s) 234
PleI GAGTC 2 cut(s) 151, 539
PluTI GGCGCC 1 cut(s) 1006
PpsI GAGTC 2 cut(s) 151, 539
Psp124BI GAGCTC 2 cut(s) 156, 981
Psp6I CCWGG 1 cut(s) 405
PspFI CCCAGC 2 cut(s) 48, 727
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 3 cut(s) 412, 465, 1004
PspPI GGNCC 2 cut(s) 250, 411
PvuI CGATCG 1 cut(s) 234
RsaI GTAC 3 cut(s) 263, 465, 503
RsaNI GTAC 3 cut(s) 262, 464, 502
SacI GAGCTC 2 cut(s) 156, 981
SaqAI TTAA 3 cut(s) 609, 831, 1122
SatI GCNGC 7 cut(s) 42, 202, 376, 379, 1006, 1020, 1031
Sau3AI GATC 2 cut(s) 231, 513
Sau96I GGNCC 2 cut(s) 250, 411
SchI GAGTC 2 cut(s) 152, 539
ScrFI CCNGG 2 cut(s) 266, 407
SduI GDGCHC 4 cut(s) 156, 766, 907, 981
SfaNI GCATC 2 cut(s) 84, 643
SfcI CTRYAG 1 cut(s) 1111
SfoI GGCGCC 1 cut(s) 1004
SinI GGWCC 1 cut(s) 250
Sse9I AATT 2 cut(s) 240, 722
SseBI AGGCCT 1 cut(s) 1058
SspDI GGCGCC 1 cut(s) 1002
SspI AATATT 2 cut(s) 703, 790
SstI GAGCTC 2 cut(s) 156, 981
StuI AGGCCT 1 cut(s) 1058
StyD4I CCNGG 2 cut(s) 264, 405
TaaI ACNGT 4 cut(s) 196, 385, 511, 1026
TaiI ACGT 2 cut(s) 608, 1120
TaqI TCGA 5 cut(s) 99, 234, 351, 479, 924
TasI AATT 2 cut(s) 240, 722
TauI GCSGC 6 cut(s) 44, 204, 378, 381, 1008, 1022
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 3 cut(s) 609, 831, 1122
Tru9I TTAA 3 cut(s) 609, 831, 1122
TscAI CASTG 1 cut(s) 1029
TseI GCWGC 1 cut(s) 1030
TspDTI ATGAA 1 cut(s) 876
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 1 cut(s) 1029
VpaK11BI GGWCC 1 cut(s) 250
XapI RAATTY 1 cut(s) 240
XceI RCATGY 1 cut(s) 328
XmnI GAANNNNTTC 3 cut(s) 301, 347, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.