Rh5CG044900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003805
Physical Location & Seq
Reverse (-)
3133833 .. 3135225
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG044900.1

Sequence Viewer

Length: 825 bp
ATGTTTGAGAATGCCCAGAAGGTGTTCGACGAAATGCCTGAGAGAAACTGCGGCCGCACCGTGTTGTCTTTCAATGCCCTCCTGGGGGCCTGCGTGAACTCCAAGAAGTTTGATATGGTTGAGAAGATTTTCGGGGAGGTACCGGAGAAGTTGTCGATTGAGCCTGATTTGGTGTCGTACAATACTGTGATCAAGGCATTTTGCAAAATGGGTTCGTTTGACTCTGCGGTTTCGGTGATTGATGTGATGGAGAAGAAGGGTGTGAAGCCGGATGTAATCACGTTCAATACGATTTTGGATTGCTTGTATGGGAATAAGCGGTTTTTGGATGCTGAGAAGATATGGGGTCAAATGGTGGAGAAGAATGTCGTTCCTAATATTAGGAGCTACAATGCCAAATTGCTGGGGTTTGCTTTGGAGAAGAGGAGTGAAGAAGGGGCTCAGTTAGTTGAAGAGTTGAGGAATATTGGAGTGAAACCTGATGTGTTCACCTTCAATGCCTTGATTAAGGGCTTTGTGAATGAGGGGAACTTGGGTGAAGCTAAGCGGTGGTATGTTGAAATAGAGAAGAGTAGTTGTGCTCCGGATAAATGCACTTTCGAGATGCTTGTTTCCTTTGTTTGTGAGAAGGATGATTTTGAGTTTTCTTTTGAGCTGTGCAAGGACATTTTCCAAAGGCGCCGCCTTGTGAGTGCGGCACTGTTGCAGCCTGTGGTGCATGGACTGGTCAAGGCCTCCATGATTGAGGAGGCAAAGGAACTTGTCAAACTTGGGAACACTAATAACTACAGACGTTTTAATCTGAAGCTGACTTCAGACAAATAG

Protein Analysis

274

Amino Acids

31.11

Weight (kDa)

6.1

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 4 - 91 2.5e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 50 - 101 3.9e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 53 - 84 1.2e-09 PPR repeat
PPR_2 PF13041 55 - 102 4.3e-16 PPR repeat family
TPR_24 PF23276 55 - 110 2.2e-06 Fungal tetratrico peptide repeats
PPR PF01535 58 - 88 9.3e-08 PPR repeat
PPR_3 PF13812 79 - 132 3.4e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 90 - 136 4.9e-10 PPR repeat family
PPR_2 PF13041 129 - 174 1.6e-06 PPR repeat family
PPR_3 PF13812 149 - 206 2.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 157 - 182 1.1e-06 PPR repeat
PPR_2 PF13041 160 - 208 8.1e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 139
AccB1I GGYRCC 2 cut(s) 139, 678
AccIII TCCGGA 1 cut(s) 583
AciI CCGC 7 cut(s) 51, 55, 227, 319, 547, 682, 695
AcoI YGGCCR 1 cut(s) 52
AcuI CTGAAG 2 cut(s) 798, 824
AcyI GRCGYC 1 cut(s) 679
AfaI GTAC 2 cut(s) 141, 179
AfiI CCNNNNNNNGG 2 cut(s) 84, 85
AgsI TTSAA 5 cut(s) 73, 286, 452, 496, 560
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 4 cut(s) 387, 542, 655, 808
AluI AGCT 4 cut(s) 387, 542, 655, 808
Alw21I GWGCWC 1 cut(s) 583
Aor13HI TCCGGA 1 cut(s) 583
AoxI GGCC 3 cut(s) 52, 87, 732
ApeKI GCWGC 1 cut(s) 706
Asp700I GAANNNNTTC 2 cut(s) 23, 128
Asp718I GGTACC 1 cut(s) 139
AspLEI GCGC 1 cut(s) 681
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 3 cut(s) 247, 481, 548
BanI GGYRCC 2 cut(s) 139, 678
BanII GRGCYC 1 cut(s) 442
Bbv12I GWGCWC 1 cut(s) 583
BbvI GCAGC 1 cut(s) 718
BccI CCATC 1 cut(s) 241
BciT130I CCWGG 1 cut(s) 83
BclI TGATCA 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 787
BfoI RGCGCY 1 cut(s) 682
BisI GCNGC 5 cut(s) 52, 55, 682, 696, 707
BlpI GCTNAGC 1 cut(s) 543
BlsI GCNGC 5 cut(s) 53, 56, 683, 697, 708
Bme1390I CCNGG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 3 cut(s) 88, 141, 680
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 2 cut(s) 319, 594
Bpu1102I GCTNAGC 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 679
BsaJI CCNNGG 1 cut(s) 82
BsaWI WCCGGW 2 cut(s) 142, 583
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 85
Bse1I ACTGG 1 cut(s) 729
BseAI TCCGGA 1 cut(s) 583
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 3 cut(s) 277, 334, 637
BseLI CCNNNNNNNGG 2 cut(s) 84, 85
BseMII CTCAG 3 cut(s) 30, 324, 455
BseNI ACTGG 1 cut(s) 729
BseRI GAGGAG 2 cut(s) 439, 761
BseX3I CGGCCG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 718
BseYI CCCAGC 1 cut(s) 403
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 3 cut(s) 54, 89, 734
BshNI GGYRCC 2 cut(s) 139, 678
BsiEI CGRYCG 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 583
BsiSI CCGG 3 cut(s) 143, 269, 584
BslI CCNNNNNNNGG 2 cut(s) 84, 85
BsmI GAATGC 1 cut(s) 16
BsnI GGCC 3 cut(s) 54, 89, 734
Bsp1286I GDGCHC 2 cut(s) 442, 583
Bsp13I TCCGGA 1 cut(s) 583
Bsp143I GATC 1 cut(s) 189
Bsp1720I GCTNAGC 1 cut(s) 543
BspACI CCGC 7 cut(s) 51, 55, 227, 319, 547, 682, 695
BspANI GGCC 3 cut(s) 54, 89, 734
BspCNI CTCAG 3 cut(s) 31, 325, 454
BspEI TCCGGA 1 cut(s) 583
BspLI GGNNCC 3 cut(s) 88, 141, 680
BspT107I GGYRCC 2 cut(s) 139, 678
BsrI ACTGG 1 cut(s) 729
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 1 cut(s) 189
BssNI GRCGYC 1 cut(s) 679
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 3 cut(s) 61, 187, 702
Bst6I CTCTTC 3 cut(s) 416, 447, 563
BstACI GRCGYC 1 cut(s) 679
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 4 cut(s) 39, 333, 441, 543
BstF5I GGATG 3 cut(s) 277, 334, 637
BstH2I RGCGCY 1 cut(s) 682
BstHHI GCGC 1 cut(s) 681
BstKTI GATC 1 cut(s) 192
BstMBI GATC 1 cut(s) 189
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 1 cut(s) 715
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstSFI CTRYAG 1 cut(s) 787
BstV1I GCAGC 1 cut(s) 718
BstXI CCANNNNNNTGG 1 cut(s) 403
BstZI CGGCCG 1 cut(s) 52
BsuRI GGCC 3 cut(s) 54, 89, 734
BtsCI GGATG 3 cut(s) 277, 334, 637
BtsIMutI CAGTG 1 cut(s) 698
Cac8I GCNNGC 1 cut(s) 91
CciNI GCGGCCGC 1 cut(s) 52
CfoI GCGC 1 cut(s) 681
Cfr13I GGNCC 1 cut(s) 87
Csp6I GTAC 2 cut(s) 140, 178
CviAII CATG 2 cut(s) 719, 739
CviQI GTAC 2 cut(s) 140, 178
DdeI CTNAG 4 cut(s) 39, 333, 441, 543
DinI GGCGCC 1 cut(s) 680
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
EaeI YGGCCR 1 cut(s) 52
EagI CGGCCG 1 cut(s) 52
Eam1104I CTCTTC 3 cut(s) 416, 447, 563
EarI CTCTTC 3 cut(s) 416, 447, 563
EclXI CGGCCG 1 cut(s) 52
Eco147I AGGCCT 1 cut(s) 734
Eco24I GRGCYC 1 cut(s) 442
Eco52I CGGCCG 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 798, 824
EcoO109I RGGNCCY 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 442
EgeI GGCGCC 1 cut(s) 680
EheI GGCGCC 1 cut(s) 680
FaeI CATG 2 cut(s) 722, 742
FaiI YATR 6 cut(s) 116, 309, 343, 555, 720, 740
FatI CATG 2 cut(s) 718, 738
FbaI TGATCA 1 cut(s) 189
Fnu4HI GCNGC 5 cut(s) 52, 55, 682, 696, 707
FokI GGATG 3 cut(s) 284, 341, 644
FriOI GRGCYC 1 cut(s) 442
Fsp4HI GCNGC 5 cut(s) 52, 55, 682, 696, 707
GlaI GCGC 1 cut(s) 680
GluI GCNGC 5 cut(s) 52, 55, 682, 696, 707
GsaI CCCAGC 1 cut(s) 407
HaeII RGCGCY 1 cut(s) 682
HaeIII GGCC 3 cut(s) 54, 89, 734
HapII CCGG 3 cut(s) 143, 269, 584
HhaI GCGC 1 cut(s) 681
Hin1I GRCGYC 1 cut(s) 679
Hin1II CATG 2 cut(s) 722, 742
Hin6I GCGC 1 cut(s) 679
HinP1I GCGC 1 cut(s) 679
HinfI GANTC 1 cut(s) 221
HpaII CCGG 3 cut(s) 143, 269, 584
HphI GGTGA 3 cut(s) 247, 481, 548
Hpy166II GTNNAC 2 cut(s) 97, 489
Hpy188I TCNGA 2 cut(s) 804, 817
Hpy188III TCNNGA 2 cut(s) 584, 601
Hpy8I GTNNAC 2 cut(s) 97, 489
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 5 cut(s) 13, 250, 428, 502, 622
HpyCH4III ACNGT 3 cut(s) 61, 187, 702
HpyCH4IV ACGT 2 cut(s) 281, 793
HpyCH4V TGCA 5 cut(s) 204, 594, 660, 706, 718
HpyF10VI GCNNNNNNNGC 1 cut(s) 715
HpyF3I CTNAG 4 cut(s) 39, 333, 441, 543
HpySE526I ACGT 2 cut(s) 281, 793
Hsp92I GRCGYC 1 cut(s) 679
Hsp92II CATG 2 cut(s) 722, 742
HspAI GCGC 1 cut(s) 679
KasI GGCGCC 1 cut(s) 678
Kpn2I TCCGGA 1 cut(s) 583
KpnI GGTACC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 1 cut(s) 189
LmnI GCTCC 2 cut(s) 384, 586
Lsp1109I GCAGC 1 cut(s) 718
LweI GCATC 2 cut(s) 319, 594
MaeII ACGT 2 cut(s) 281, 793
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 8 cut(s) 136, 265, 349, 373, 433, 443, 464, 580
MhlI GDGCHC 2 cut(s) 442, 583
MluCI AATT 1 cut(s) 398
Mly113I GGCGCC 1 cut(s) 679
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 8 cut(s) 89, 130, 417, 453, 517, 739, 742, 745
MroI TCCGGA 1 cut(s) 583
MroXI GAANNNNTTC 2 cut(s) 23, 128
MseI TTAA 2 cut(s) 507, 798
MspI CCGG 3 cut(s) 143, 269, 584
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 16
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 715
NarI GGCGCC 1 cut(s) 679
NdeII GATC 1 cut(s) 189
NlaIII CATG 2 cut(s) 722, 742
NlaIV GGNNCC 3 cut(s) 88, 141, 680
NotI GCGGCCGC 1 cut(s) 52
PceI AGGCCT 1 cut(s) 734
PcsI WCGNNNNNNNCGW 1 cut(s) 287
PctI GAATGC 1 cut(s) 16
PdmI GAANNNNTTC 2 cut(s) 23, 128
PkrI GCNGC 5 cut(s) 53, 56, 683, 697, 708
PleI GAGTC 1 cut(s) 215
PluTI GGCGCC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 81
PspFI CCCAGC 1 cut(s) 403
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 3 cut(s) 88, 141, 680
PspPI GGNCC 1 cut(s) 87
RsaI GTAC 2 cut(s) 141, 179
RsaNI GTAC 2 cut(s) 140, 178
SaqAI TTAA 2 cut(s) 507, 798
SatI GCNGC 5 cut(s) 52, 55, 682, 696, 707
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 2 cut(s) 442, 583
SfaNI GCATC 2 cut(s) 319, 594
SfcI CTRYAG 1 cut(s) 787
SfoI GGCGCC 1 cut(s) 680
Sse9I AATT 1 cut(s) 398
SseBI AGGCCT 1 cut(s) 734
SsiI CCGC 7 cut(s) 51, 55, 227, 319, 547, 682, 695
SspDI GGCGCC 1 cut(s) 678
SspI AATATT 2 cut(s) 379, 466
StuI AGGCCT 1 cut(s) 734
StyD4I CCNGG 1 cut(s) 81
TaaI ACNGT 3 cut(s) 61, 187, 702
TaiI ACGT 2 cut(s) 284, 796
TaqI TCGA 3 cut(s) 27, 155, 600
TasI AATT 1 cut(s) 398
TauI GCSGC 4 cut(s) 54, 57, 684, 698
Tru1I TTAA 2 cut(s) 507, 798
Tru9I TTAA 2 cut(s) 507, 798
TscAI CASTG 1 cut(s) 705
TseI GCWGC 1 cut(s) 706
TspRI CASTG 1 cut(s) 705
XmnI GAANNNNTTC 2 cut(s) 23, 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.