RLG00000031258

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003805
Physical Location & Seq
Reverse (-)
3444575 .. 3446441
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031258

Sequence Viewer

Length: 765 bp
ATGTTGTATGGTCACCTCCTTCGCCGTGCATTTTCCACCGCCCCCACCACCACCCAGCCCAAACCCACAACCGCCAAGAGCATCAGAGCCATCTCCGCCAACTTCGCCAATGTCAAGCACTTCCTTGCGATCGATGAAATCCTAGAGGACCAGAAGAAGTACCCGGATTTCTCCGACGAGGGTTTCACCGTCCGAATCATTTCCCTTTATGGAAAGGCCGGCATGTTTGATAATGCCCGAAAGGTGTACGATGAAATCCTTATAGAAACTATTTTCGGGGAGGTGTCGGAGATGTGGTCGATTGAGCCTGATTTGGTGTCATACAATATTGTGATCAAGGCATTTTGCCAGATGGATTTGTTTGACTCTGCGGTTTCGGTGATTGAGATGATGAATAAGAATGGTGTGAAGCCGGATGTAATCACGTTTAATACGATTTTGGATTACTTGTATGGGAGTAAGCGATTTTCAGATGCTGAGAAGATATGGGGTCAAATGGTGGAGAATAATGTTGTTCCTGATATTAGGAGCTACAATGCCAAGTTGCTGGGGTTGGGTTCGGAGAAGAGGAGTGAAGAAGGGGCTCAACTAGTTGAAGAGTTGAGGAGTATTGGAGTGAAACCTAATGTGTTCACCTTTAATACCTTGATTAAGAGCAAGTTCACCGGGCTCTGTTATGCCCGGCCTATGGTGGATGGTCTAGTCAAGGCCTCCATGATTGAGGAGGCAAAGGAGCTTGTCAAACTTGGGAACACTAATAATTAA

Protein Analysis

255

Amino Acids

28.57

Weight (kDa)

5.6

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 101 - 131 5.1e-07 PPR repeat
PPR_3 PF13812 101 - 148 2.7e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 103 - 148 3.8e-13 PPR repeat family
PPR PF01535 106 - 136 2.7e-06 PPR repeat
PPR_3 PF13812 127 - 180 9.7e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 138 - 180 4.3e-10 PPR repeat family
PPR_2 PF13041 173 - 218 2.7e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 39, 72, 96, 371
AfaI GTAC 2 cut(s) 161, 248
AfiI CCNNNNNNNGG 1 cut(s) 688
AgsI TTSAA 1 cut(s) 596
AhlI ACTAGT 1 cut(s) 589
AluBI AGCT 2 cut(s) 531, 736
AluI AGCT 2 cut(s) 531, 736
AlwNI CAGNNNCTG 1 cut(s) 476
AoxI GGCC 3 cut(s) 216, 683, 708
Asp700I GAANNNNTTC 1 cut(s) 199
AspS9I GGNCC 1 cut(s) 148
AsuC2I CCSGG 3 cut(s) 164, 667, 682
AsuHPI GGTGA 5 cut(s) 5, 178, 391, 625, 655
AvaII GGWCC 1 cut(s) 148
BanII GRGCYC 2 cut(s) 586, 672
BccI CCATC 3 cut(s) 98, 346, 689
BceAI ACGGC 1 cut(s) 9
BclI TGATCA 1 cut(s) 333
BcnI CCSGG 3 cut(s) 164, 667, 682
BcuI ACTAGT 1 cut(s) 589
BfaI CTAG 3 cut(s) 143, 590, 701
Bme1390I CCNGG 3 cut(s) 164, 667, 682
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 148
BmrFI CCNGG 3 cut(s) 164, 667, 682
BmsI GCATC 2 cut(s) 90, 463
BpuMI CCSGG 3 cut(s) 164, 667, 682
Bsa29I ATCGAT 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 1 cut(s) 688
Bse118I RCCGGY 1 cut(s) 218
BseCI ATCGAT 1 cut(s) 132
BseGI GGATG 2 cut(s) 421, 700
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMII CTCAG 1 cut(s) 468
BseRI GAGGAG 3 cut(s) 583, 619, 737
BseYI CCCAGC 2 cut(s) 54, 547
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 218, 685, 710
BshVI ATCGAT 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 132
BsiSI CCGG 5 cut(s) 164, 219, 413, 666, 682
BslI CCNNNNNNNGG 1 cut(s) 688
BsnI GGCC 3 cut(s) 218, 685, 710
Bsp1286I GDGCHC 2 cut(s) 586, 672
Bsp143I GATC 2 cut(s) 129, 333
BspACI CCGC 4 cut(s) 39, 72, 96, 371
BspANI GGCC 3 cut(s) 218, 685, 710
BspCNI CTCAG 1 cut(s) 469
BspDI ATCGAT 1 cut(s) 132
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssMI GATC 2 cut(s) 129, 333
Bst4CI ACNGT 1 cut(s) 190
Bst6I CTCTTC 2 cut(s) 560, 591
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 1 cut(s) 477
BstEII GGTNACC 1 cut(s) 11
BstF5I GGATG 2 cut(s) 421, 700
BstKTI GATC 2 cut(s) 132, 336
BstMBI GATC 2 cut(s) 129, 333
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 95, 104
BstNSI RCATGY 1 cut(s) 226
BstPI GGTNACC 1 cut(s) 11
BstSCI CCNGG 3 cut(s) 162, 665, 680
BstXI CCANNNNNNTGG 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 132
BsuRI GGCC 3 cut(s) 218, 685, 710
BsuTUI ATCGAT 1 cut(s) 132
BtsCI GGATG 2 cut(s) 421, 700
Cac8I GCNNGC 1 cut(s) 220
CaiI CAGNNNCTG 1 cut(s) 476
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 148
ClaI ATCGAT 1 cut(s) 132
Csp6I GTAC 2 cut(s) 160, 247
CviAII CATG 2 cut(s) 223, 715
CviQI GTAC 2 cut(s) 160, 247
DdeI CTNAG 1 cut(s) 477
DpnI GATC 2 cut(s) 131, 335
DpnII GATC 2 cut(s) 129, 333
Eam1104I CTCTTC 2 cut(s) 560, 591
EarI CTCTTC 2 cut(s) 560, 591
EciI GGCGGA 1 cut(s) 85
Eco147I AGGCCT 1 cut(s) 710
Eco24I GRGCYC 2 cut(s) 586, 672
Eco47I GGWCC 1 cut(s) 148
Eco91I GGTNACC 1 cut(s) 11
EcoO65I GGTNACC 1 cut(s) 11
EcoT38I GRGCYC 2 cut(s) 586, 672
FaeI CATG 2 cut(s) 226, 718
FatI CATG 2 cut(s) 222, 714
FbaI TGATCA 1 cut(s) 333
FokI GGATG 2 cut(s) 428, 707
FriOI GRGCYC 2 cut(s) 586, 672
FspBI CTAG 3 cut(s) 143, 590, 701
GsaI CCCAGC 2 cut(s) 58, 551
HaeIII GGCC 3 cut(s) 218, 685, 710
HapII CCGG 5 cut(s) 164, 219, 413, 666, 682
Hin1II CATG 2 cut(s) 226, 718
HinfI GANTC 2 cut(s) 195, 365
HpaII CCGG 5 cut(s) 164, 219, 413, 666, 682
HphI GGTGA 5 cut(s) 5, 178, 391, 625, 655
Hpy166II GTNNAC 3 cut(s) 247, 633, 663
Hpy188I TCNGA 6 cut(s) 86, 175, 194, 289, 472, 562
Hpy188III TCNNGA 1 cut(s) 518
Hpy8I GTNNAC 3 cut(s) 247, 633, 663
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 2 cut(s) 29, 572
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 425
HpyCH4V TGCA 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 104
HpyF3I CTNAG 1 cut(s) 477
HpySE526I ACGT 1 cut(s) 425
Hsp92II CATG 2 cut(s) 226, 718
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 333
Kzo9I GATC 2 cut(s) 129, 333
LmnI GCTCC 2 cut(s) 528, 733
LweI GCATC 2 cut(s) 90, 463
MaeI CTAG 3 cut(s) 143, 590, 701
MaeII ACGT 1 cut(s) 425
MaeIII GTNAC 1 cut(s) 11
MalI GATC 2 cut(s) 131, 335
MboI GATC 2 cut(s) 129, 333
MboII GAAGA 5 cut(s) 166, 493, 577, 587, 608
MhlI GDGCHC 2 cut(s) 586, 672
MluCI AATT 1 cut(s) 760
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 198, 267
MnlI CCTC 9 cut(s) 26, 139, 172, 274, 561, 597, 715, 718, 721
MroNI GCCGGC 1 cut(s) 218
MroXI GAANNNNTTC 1 cut(s) 199
MseI TTAA 4 cut(s) 429, 639, 651, 763
MspI CCGG 5 cut(s) 164, 219, 413, 666, 682
MspR9I CCNGG 3 cut(s) 164, 667, 682
MwoI GCNNNNNNNGC 2 cut(s) 95, 104
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 3 cut(s) 164, 667, 682
NdeII GATC 2 cut(s) 129, 333
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 2 cut(s) 226, 718
NmuCI GTSAC 1 cut(s) 11
NspI RCATGY 1 cut(s) 226
PceI AGGCCT 1 cut(s) 710
PcsI WCGNNNNNNNCGW 1 cut(s) 431
PdiI GCCGGC 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 195
Ple19I CGATCG 1 cut(s) 132
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
PspEI GGTNACC 1 cut(s) 11
PspFI CCCAGC 2 cut(s) 54, 547
PspPI GGNCC 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 476
PvuI CGATCG 1 cut(s) 132
RsaI GTAC 2 cut(s) 161, 248
RsaNI GTAC 2 cut(s) 160, 247
SaqAI TTAA 4 cut(s) 429, 639, 651, 763
Sau3AI GATC 2 cut(s) 129, 333
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 3 cut(s) 164, 667, 682
SduI GDGCHC 2 cut(s) 586, 672
SetI ASST 9 cut(s) 18, 246, 285, 428, 533, 625, 638, 647, 738
SfaNI GCATC 2 cut(s) 90, 463
SinI GGWCC 1 cut(s) 148
SpeI ACTAGT 1 cut(s) 589
Sse9I AATT 1 cut(s) 760
SseBI AGGCCT 1 cut(s) 710
SsiI CCGC 4 cut(s) 39, 72, 96, 371
SspI AATATT 1 cut(s) 328
SspMI CTAG 3 cut(s) 143, 590, 701
StuI AGGCCT 1 cut(s) 710
StyD4I CCNGG 3 cut(s) 162, 665, 680
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 1 cut(s) 428
TaqI TCGA 2 cut(s) 132, 299
TasI AATT 1 cut(s) 760
TfiI GAWTC 1 cut(s) 195
Tru1I TTAA 4 cut(s) 429, 639, 651, 763
Tru9I TTAA 4 cut(s) 429, 639, 651, 763
TseFI GTSAC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 3 cut(s) 150, 267, 407
VpaK11BI GGWCC 1 cut(s) 148
XceI RCATGY 1 cut(s) 226
XmnI GAANNNNTTC 1 cut(s) 199
XspI CTAG 3 cut(s) 143, 590, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.