pycom05g30630

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003805
Physical Location & Seq
Forward (+)
30690785 .. 30691933
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30630.1

Sequence Viewer

Length: 1149 bp
ATGTCGTTCTCTCGCGTCCTCCAGCGCGCCTTCTGCACCGCCACCCAATCCAACGCCGCCACCACCACCAACAGCATTGGCGCCATCTCCACCGACCTCTACAAAGAGCGCAACCTGAAACGCCTCGTCGAGAAGTTTAAGAAGTCCTCCGAGCTCTACCGCTTCCGTACCAAAACCCCCATTTACGAGGACACCGTCCGCCGTCTCGCAGCCGCCAAACGTTTCAAATGGATCGAGGAGATCCTGGAGGACCAGAAGCAGTACCCGGACTTTTCCACGGAGGGATTCGCTGTCCGACTGGTTACGCTTTACGGGAAAGCTGGCATGTTCGAAAATGCCGAGAAGGTGTTCGACGAAATGCCTGAGAAAAGCTGTGAGCGCACCGTCTTGTCCTTTAATGCCCTCTTGGGTGCTTGTGTTAACTCCAAGAAATTCGACAAGGTTGATGAGGTTTTTAGAGGGGTCTCGCAGAAGTTGTCAATTGAGCCGGATGTGGTGTCGTATAATACTGTGGTCAAAGCGTTTTGTGAAATGGGTTCGTTGGAATCGGCGGTTTCAATGCTTGTTGAGATGGAGAATAAGGGTGTTGAGCCGGATGTGATCACATTCAACACGATCATGAATGGGTTGTATGTGAATAATCAGTTTTTGGCAGCCGAAAAAATATGGAGTCGAATGGTGGAGAAGAATGTTGTGCCTGATGTTAGGAGCTACAATGCCAGGTTGCTGGGATTGGCTTCGGAGAAGAAAACTAACGAGGCGGTTGAGTTATTCGAGGAAATGAGGAACAAGGAAGTGAAACCTGATGTTTTTAGCTTCAATGCCTTGATCAAGGGATTTGTTAATGAGGGGAAATTGGAAGAAGCTAAACAGTGGTACCGAGAACTGGTGAAGAGTAGTTGTGCTCTGCAAAAATGGACTTTCCAGATGCTTGTTCCCTTTGTTTGCGAGAAGGGTGATTTGGACTTTGCCTTTGAACTCAGCGAGGAAATTTTCAAAAGGCGCCTCCTTGTCGATGTGGAACTATTGCAGGTTGTGGTGGACGGGCTGGCTAAGGCTTCCAGAATCAATGAAGCAAAGAAGATTGTGAAGCTTGGGAACACAAATACTTACCGGCGTTACAATTTGCAGTTGCCTTCGGAGGACTAA

Protein Analysis

383

Amino Acids

43.79

Weight (kDa)

8.04

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 100 - 141 2e-06 PPR repeat family
PPR_3 PF13812 100 - 141 2.7e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 158 - 208 2.6e-10 Pentatricopeptide repeat domain
TPR_24 PF23276 159 - 260 1.4e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 161 - 192 2.1e-09 PPR repeat
PPR_2 PF13041 163 - 210 7.3e-16 PPR repeat family
PPR PF01535 166 - 196 3.8e-06 PPR repeat
PPR_long PF17177 188 - 296 1.8e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 198 - 246 6.3e-11 PPR repeat family
PPR_3 PF13812 222 - 282 2.1e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 233 - 282 4.9e-13 PPR repeat family
PPR_1 PF12854 264 - 291 6.5e-06 PPR repeat
PPR_2 PF13041 268 - 312 1.3e-08 PPR repeat family
PPR PF01535 271 - 301 8.1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1021
Acc65I GGTACC 1 cut(s) 876
AccB1I GGYRCC 3 cut(s) 80, 876, 1002
AccII CGCG 2 cut(s) 15, 27
AciI CCGC 7 cut(s) 39, 57, 160, 199, 213, 551, 761
AclI AACGTT 1 cut(s) 220
AclWI GGATC 2 cut(s) 235, 239
AcsI RAATTY 2 cut(s) 431, 990
AcyI GRCGYC 2 cut(s) 81, 1003
AfaI GTAC 3 cut(s) 169, 263, 878
AfiI CCNNNNNNNGG 1 cut(s) 886
AgsI TTSAA 6 cut(s) 226, 558, 610, 820, 977, 997
AjnI CCWGG 2 cut(s) 243, 719
AjuI GAANNNNNNNTTGG 2 cut(s) 944, 976
AluBI AGCT 7 cut(s) 154, 320, 372, 711, 816, 866, 1093
AluI AGCT 7 cut(s) 154, 320, 372, 711, 816, 866, 1093
Alw21I GWGCWC 2 cut(s) 156, 907
Alw26I GTCTC 2 cut(s) 209, 469
AlwI GGATC 2 cut(s) 235, 239
ApeKI GCWGC 2 cut(s) 209, 653
ApoI RAATTY 2 cut(s) 431, 990
ArsI GACNNNNNNTTYG 2 cut(s) 956, 988
Asp700I GAANNNNTTC 1 cut(s) 347
Asp718I GGTACC 1 cut(s) 876
AspLEI GCGC 6 cut(s) 27, 29, 83, 111, 381, 1005
AspS9I GGNCC 1 cut(s) 250
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 2 cut(s) 901, 968
AsuII TTCGAA 1 cut(s) 330
AvaII GGWCC 1 cut(s) 250
BaeI ACNNNNGTAYC 2 cut(s) 860, 893
BanI GGYRCC 3 cut(s) 80, 876, 1002
BanII GRGCYC 1 cut(s) 156
Bbv12I GWGCWC 2 cut(s) 156, 907
BbvI GCAGC 2 cut(s) 221, 665
BccI CCATC 2 cut(s) 92, 565
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 2 cut(s) 245, 721
BclI TGATCA 2 cut(s) 600, 828
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 2 cut(s) 209, 469
BfoI RGCGCY 2 cut(s) 84, 1006
BfuAI ACCTGC 1 cut(s) 1021
BisI GCNGC 4 cut(s) 57, 210, 213, 654
BlsI GCNGC 4 cut(s) 58, 211, 214, 655
Bme1390I CCNGG 3 cut(s) 245, 266, 721
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 3 cut(s) 82, 878, 1004
BmrFI CCNGG 3 cut(s) 245, 266, 721
BmsI GCATC 1 cut(s) 918
BpmI CTGGAG 2 cut(s) 5, 266
Bpu10I CCTNAGC 1 cut(s) 1053
Bpu14I TTCGAA 1 cut(s) 330
BpuMI CCSGG 1 cut(s) 266
BsaHI GRCGYC 2 cut(s) 81, 1003
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 1 cut(s) 276
Bsc4I CCNNNNNNNGG 1 cut(s) 886
Bse118I RCCGGY 1 cut(s) 1113
Bse1I ACTGG 2 cut(s) 303, 891
BseBI CCWGG 2 cut(s) 245, 721
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 2 cut(s) 496, 601
BseLI CCNNNNNNNGG 1 cut(s) 886
BseMII CTCAG 2 cut(s) 354, 994
BseNI ACTGG 2 cut(s) 303, 891
BsePI GCGCGC 1 cut(s) 25
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 2 cut(s) 221, 665
BseYI CCCAGC 1 cut(s) 727
BsgI GTGCAG 1 cut(s) 19
Bsh1236I CGCG 2 cut(s) 15, 27
BshNI GGYRCC 3 cut(s) 80, 876, 1002
BsiHKAI GWGCWC 2 cut(s) 156, 907
BsiSI CCGG 4 cut(s) 266, 488, 593, 1114
BslI CCNNNNNNNGG 1 cut(s) 886
BsmAI GTCTC 2 cut(s) 209, 469
BsmBI CGTCTC 1 cut(s) 209
Bso31I GGTCTC 1 cut(s) 469
Bsp119I TTCGAA 1 cut(s) 330
Bsp1286I GDGCHC 2 cut(s) 156, 907
Bsp143I GATC 5 cut(s) 231, 240, 600, 615, 828
BspACI CCGC 7 cut(s) 39, 57, 160, 199, 213, 551, 761
BspCNI CTCAG 2 cut(s) 355, 993
BspFNI CGCG 2 cut(s) 15, 27
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 3 cut(s) 82, 878, 1004
BspMI ACCTGC 1 cut(s) 1021
BspPI GGATC 2 cut(s) 235, 239
BspT104I TTCGAA 1 cut(s) 330
BspT107I GGYRCC 3 cut(s) 80, 876, 1002
BspTNI GGTCTC 1 cut(s) 469
BsrFI RCCGGY 1 cut(s) 1113
BsrI ACTGG 2 cut(s) 303, 891
BssAI RCCGGY 1 cut(s) 1113
BssECI CCNNGG 1 cut(s) 276
BssHII GCGCGC 1 cut(s) 25
BssMI GATC 5 cut(s) 231, 240, 600, 615, 828
BssNI GRCGYC 2 cut(s) 81, 1003
Bst2UI CCWGG 2 cut(s) 245, 721
Bst4CI ACNGT 4 cut(s) 196, 385, 511, 873
Bst6I CTCTTC 1 cut(s) 887
BstACI GRCGYC 2 cut(s) 81, 1003
BstBI TTCGAA 1 cut(s) 330
BstC8I GCNNGC 3 cut(s) 27, 322, 1050
BstDEI CTNAG 3 cut(s) 363, 980, 1053
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 2 cut(s) 496, 601
BstFNI CGCG 2 cut(s) 15, 27
BstH2I RGCGCY 2 cut(s) 84, 1006
BstHHI GCGC 6 cut(s) 27, 29, 83, 111, 381, 1005
BstKTI GATC 5 cut(s) 234, 243, 603, 618, 831
BstMAI GTCTC 2 cut(s) 209, 469
BstMBI GATC 5 cut(s) 231, 240, 600, 615, 828
BstMWI GCNNNNNNNGC 2 cut(s) 33, 378
BstNI CCWGG 2 cut(s) 245, 721
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 3 cut(s) 243, 264, 719
BstUI CGCG 2 cut(s) 15, 27
BstV1I GCAGC 2 cut(s) 221, 665
BstX2I RGATCY 1 cut(s) 240
BstXI CCANNNNNNTGG 1 cut(s) 727
BstYI RGATCY 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 2 cut(s) 496, 601
BtsIMutI CAGTG 1 cut(s) 878
BveI ACCTGC 1 cut(s) 1021
Cac8I GCNNGC 3 cut(s) 27, 322, 1050
CciI TCATGA 1 cut(s) 618
CfoI GCGC 6 cut(s) 27, 29, 83, 111, 381, 1005
Cfr10I RCCGGY 1 cut(s) 1113
Cfr13I GGNCC 1 cut(s) 250
CseI GACGC 1 cut(s) 4
Csp6I GTAC 3 cut(s) 168, 262, 877
CviAII CATG 2 cut(s) 325, 619
CviQI GTAC 3 cut(s) 168, 262, 877
DdeI CTNAG 3 cut(s) 363, 980, 1053
DinI GGCGCC 2 cut(s) 82, 1004
DpnI GATC 5 cut(s) 233, 242, 602, 617, 830
DpnII GATC 5 cut(s) 231, 240, 600, 615, 828
Eam1104I CTCTTC 1 cut(s) 887
EarI CTCTTC 1 cut(s) 887
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 154
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 243, 719
EcoT38I GRGCYC 1 cut(s) 156
EgeI GGCGCC 2 cut(s) 82, 1004
EheI GGCGCC 2 cut(s) 82, 1004
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 2 cut(s) 328, 622
FaiI YATR 5 cut(s) 326, 504, 620, 633, 667
FatI CATG 2 cut(s) 324, 618
FbaI TGATCA 2 cut(s) 600, 828
Fnu4HI GCNGC 4 cut(s) 57, 210, 213, 654
FokI GGATG 2 cut(s) 503, 608
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 4 cut(s) 57, 210, 213, 654
GlaI GCGC 6 cut(s) 26, 28, 82, 110, 380, 1004
GluI GCNGC 4 cut(s) 57, 210, 213, 654
GsaI CCCAGC 1 cut(s) 731
GsuI CTGGAG 2 cut(s) 5, 266
HaeII RGCGCY 2 cut(s) 84, 1006
HapII CCGG 4 cut(s) 266, 488, 593, 1114
HgaI GACGC 1 cut(s) 4
HhaI GCGC 6 cut(s) 27, 29, 83, 111, 381, 1005
Hin1I GRCGYC 2 cut(s) 81, 1003
Hin1II CATG 2 cut(s) 328, 622
Hin6I GCGC 6 cut(s) 25, 27, 81, 109, 379, 1003
HinP1I GCGC 6 cut(s) 25, 27, 81, 109, 379, 1003
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 1091
HinfI GANTC 4 cut(s) 285, 545, 670, 1065
HpaI GTTAAC 1 cut(s) 421
HpaII CCGG 4 cut(s) 266, 488, 593, 1114
HphI GGTGA 2 cut(s) 901, 968
Hpy166II GTNNAC 2 cut(s) 421, 1042
Hpy188I TCNGA 4 cut(s) 151, 296, 742, 1141
Hpy188III TCNNGA 4 cut(s) 130, 619, 925, 1062
Hpy8I GTNNAC 2 cut(s) 421, 1042
Hpy99I CGWCG 2 cut(s) 131, 356
HpyAV CCTTC 4 cut(s) 40, 337, 946, 1146
HpyCH4III ACNGT 4 cut(s) 196, 385, 511, 873
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 4 cut(s) 36, 910, 1030, 1129
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 378
HpyF3I CTNAG 3 cut(s) 363, 980, 1053
HpySE526I ACGT 1 cut(s) 220
Hsp92I GRCGYC 2 cut(s) 81, 1003
Hsp92II CATG 2 cut(s) 328, 622
HspAI GCGC 6 cut(s) 25, 27, 81, 109, 379, 1003
KasI GGCGCC 2 cut(s) 80, 1002
KpnI GGTACC 1 cut(s) 880
Ksp22I TGATCA 2 cut(s) 600, 828
KspAI GTTAAC 1 cut(s) 421
Kzo9I GATC 5 cut(s) 231, 240, 600, 615, 828
LmnI GCTCC 1 cut(s) 708
Lsp1109I GCAGC 2 cut(s) 221, 665
LweI GCATC 1 cut(s) 918
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 2 cut(s) 301, 1118
MalI GATC 5 cut(s) 233, 242, 602, 617, 830
MboI GATC 5 cut(s) 231, 240, 600, 615, 828
MboII GAAGA 5 cut(s) 697, 757, 872, 904, 1093
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 1 cut(s) 240
MhlI GDGCHC 2 cut(s) 156, 907
MluCI AATT 5 cut(s) 431, 480, 854, 990, 1123
Mly113I GGCGCC 2 cut(s) 81, 1003
MlyI GAGTC 1 cut(s) 679
MmeI TCCRAC 3 cut(s) 75, 319, 522
MroXI GAANNNNTTC 1 cut(s) 347
MseI TTAA 4 cut(s) 138, 396, 420, 843
MslI CAYNNNNRTG 1 cut(s) 617
MspI CCGG 4 cut(s) 266, 488, 593, 1114
MspR9I CCNGG 3 cut(s) 245, 266, 721
MunI CAATTG 1 cut(s) 480
MvaI CCWGG 2 cut(s) 245, 721
MvnI CGCG 2 cut(s) 15, 27
MwoI GCNNNNNNNGC 2 cut(s) 33, 378
NarI GGCGCC 2 cut(s) 81, 1003
NciI CCSGG 1 cut(s) 266
NdeII GATC 5 cut(s) 231, 240, 600, 615, 828
NlaIII CATG 2 cut(s) 328, 622
NlaIV GGNNCC 3 cut(s) 82, 878, 1004
NmeAIII GCCGAG 1 cut(s) 364
NspI RCATGY 1 cut(s) 328
NspV TTCGAA 1 cut(s) 330
PagI TCATGA 1 cut(s) 618
PauI GCGCGC 1 cut(s) 25
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 336
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 3 cut(s) 285, 545, 1065
PflFI GACNNNGTC 1 cut(s) 194
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 4 cut(s) 58, 211, 214, 655
PleI GAGTC 1 cut(s) 678
PluTI GGCGCC 2 cut(s) 84, 1006
PpsI GAGTC 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 156
Psp1406I AACGTT 1 cut(s) 220
Psp6I CCWGG 2 cut(s) 243, 719
PspFI CCCAGC 1 cut(s) 727
PspGI CCWGG 2 cut(s) 243, 719
PspN4I GGNNCC 3 cut(s) 82, 878, 1004
PspPI GGNCC 1 cut(s) 250
PsuI RGATCY 1 cut(s) 240
PsyI GACNNNGTC 1 cut(s) 194
PteI GCGCGC 1 cut(s) 25
RsaI GTAC 3 cut(s) 169, 263, 878
RsaNI GTAC 3 cut(s) 168, 262, 877
RseI CAYNNNNRTG 1 cut(s) 617
SacI GAGCTC 1 cut(s) 156
SaqAI TTAA 4 cut(s) 138, 396, 420, 843
SatI GCNGC 4 cut(s) 57, 210, 213, 654
Sau3AI GATC 5 cut(s) 231, 240, 600, 615, 828
Sau96I GGNCC 1 cut(s) 250
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 3 cut(s) 245, 266, 721
SduI GDGCHC 2 cut(s) 156, 907
SfaNI GCATC 1 cut(s) 918
SfoI GGCGCC 2 cut(s) 82, 1004
SfuI TTCGAA 1 cut(s) 330
SinI GGWCC 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 617
Sse9I AATT 5 cut(s) 431, 480, 854, 990, 1123
SsiI CCGC 7 cut(s) 39, 57, 160, 199, 213, 551, 761
SspDI GGCGCC 2 cut(s) 80, 1002
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 243, 264, 719
TaaI ACNGT 4 cut(s) 196, 385, 511, 873
TaiI ACGT 1 cut(s) 223
TaqI TCGA 8 cut(s) 129, 234, 330, 351, 435, 673, 774, 1014
TasI AATT 5 cut(s) 431, 480, 854, 990, 1123
TauI GCSGC 2 cut(s) 59, 215
TfiI GAWTC 3 cut(s) 285, 545, 1065
Tru1I TTAA 4 cut(s) 138, 396, 420, 843
Tru9I TTAA 4 cut(s) 138, 396, 420, 843
TscAI CASTG 1 cut(s) 878
TseI GCWGC 2 cut(s) 209, 653
TspDTI ATGAA 2 cut(s) 635, 1086
TspGWI ACGGA 2 cut(s) 155, 293
TspRI CASTG 1 cut(s) 878
Tth111I GACNNNGTC 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 250
XapI RAATTY 2 cut(s) 431, 990
XceI RCATGY 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.