FvH4_3g04430

PAP-specific phosphatase HAL2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002929
Physical Location & Seq
Forward (+)
2542466 .. 2545728
3263 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04430.t5

Sequence Viewer

Length: 1086 bp
ATGCCTTTACATTGTTCTAGTACTATGGCTATAAAAGCCTCTCACAATCTAGGACTTGTGAAATGGAATAACAAGCACAAGTTCATAACCGACCACGTAGGGACAGTTAGCTTCTACAAAATCCCCCACCACAAAATCTCAAAACCAATTGGTTGCTTATCCAAGCTCACCCAAACCCAGTCCTTCACAGTAATGGAGGACCCAAAAAGGTCGAGTCTTTCATCCCTACAACCAACCCATGATTTGGAAATTGCAGTCAGAGCTGTGCAAATGGCTTGTTCTCTCTGCCAGAAAGTTCAGGACAGTTTGGTTTCCAAGTCCGGCGCTGTGGTCCAATCCAAAGATGACAACTCTCCAGTCACTGTTGCAGATTGGAGTGTCCAAGCAACTGTGAGCTGGATACTGTCGGAGTCTTTTGGAAGTAGCAATGTATCCATTGTAGCTGAAGAAGACATTCAAAACCTGTCCAAGGCTAAGGCAGCTGGAATGTTGGAAGCAGTAGTAAAAACTGTAAATGTTTGTCTAGCTGAAGCTCCTCGTTTTGGACTTCAAGGTCCTAAAAAGACCCTTGGGACTAAAGAGGTTCTTGAAGCCATCAGTCGGTGCAACTCAACGGGAGGCCCTACTGGGCGATTTTGGGCTCTTGACCCTGTTGATGGAACATTGGGATTTGTACGAGGGGATCAATATGCTATTGCTCTGGCTCTAATAGAGGATGGAGAAGTTGTGCTTGGAGTTCTTGGATGCCCTAATTACCCAATGCGAAGAGAATGGCTAAACTATCATCACAGGTATCATAGAATTATATCCAAATTGACCCCACCAACATCAGAATCATGGGACAAGGGCTGTGTGTTATACGCTAGAAGAGGTAGCAGTGAAGCTTGGATGCAACCACTGCTCCATGTAAATAAGAAGTTTCTGTGGCCAAACTCTGCAAAACTGGTCCAAGTGTCCTCTATCAATAATCCATCATTGGCAACCTTTTGTGAACCGGTTGAGAAGGCAAATTCAAGTCATTCCTTCACAGCAGGACTAGCTCACAGCGTTGGACTTAGGTACGAAGCAACCATTAAGAGTCTATAG

Protein Analysis

362

Amino Acids

39.47

Weight (kDa)

8.96

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inositol_P PF00459 6 - 327 3.8e-42 Inositol monophosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 552
AccB7I CCANNNNNTGG 1 cut(s) 244
AclWI GGATC 1 cut(s) 690
AcoI YGGCCR 1 cut(s) 926
AcsI RAATTY 1 cut(s) 1009
AcuI CTGAAG 2 cut(s) 465, 549
AfaI GTAC 3 cut(s) 22, 675, 1061
AfiI CCNNNNNNNGG 5 cut(s) 244, 469, 542, 600, 656
AgeI ACCGGT 1 cut(s) 994
AgsI TTSAA 4 cut(s) 458, 551, 590, 1014
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
AlwI GGATC 1 cut(s) 690
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 2 cut(s) 619, 926
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 1009
AsiGI ACCGGT 1 cut(s) 994
Asp700I GAANNNNTTC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 326
AspS9I GGNCC 5 cut(s) 199, 331, 554, 620, 946
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 4 cut(s) 199, 331, 554, 946
BalI TGGCCA 1 cut(s) 928
BanII GRGCYC 1 cut(s) 643
BbsI GAAGAC 1 cut(s) 456
BbvI GCAGC 1 cut(s) 491
BccI CCATC 4 cut(s) 602, 650, 710, 979
BciVI GTATCC 2 cut(s) 393, 442
BfaI CTAG 5 cut(s) 18, 50, 524, 864, 1037
BfmI CTRYAG 1 cut(s) 1082
BfoI RGCGCY 1 cut(s) 327
BfuI GTATCC 2 cut(s) 393, 442
BisI GCNGC 1 cut(s) 480
BlsI GCNGC 1 cut(s) 481
BmcAI AGTACT 1 cut(s) 22
Bme18I GGWCC 4 cut(s) 199, 331, 554, 946
BmgT120I GGNCC 5 cut(s) 199, 331, 554, 620, 946
BmiI GGNNCC 1 cut(s) 201
BmrI ACTGGG 2 cut(s) 172, 636
BmsI GCATC 2 cut(s) 734, 879
BmuI ACTGGG 2 cut(s) 172, 636
BpiI GAAGAC 1 cut(s) 456
BpmI CTGGAG 1 cut(s) 339
Bpu10I CCTNAGC 1 cut(s) 474
BsaAI YACGTR 1 cut(s) 97
BsaJI CCNNGG 2 cut(s) 468, 568
BsaWI WCCGGW 1 cut(s) 994
BsaXI ACNNNNNCTCC 4 cut(s) 711, 741, 885, 915
Bsc4I CCNNNNNNNGG 5 cut(s) 244, 469, 542, 600, 656
Bse118I RCCGGY 1 cut(s) 994
Bse1I ACTGG 4 cut(s) 178, 356, 631, 948
Bse3DI GCAATG 1 cut(s) 433
BseDI CCNNGG 2 cut(s) 468, 568
BseGI GGATG 4 cut(s) 221, 721, 749, 894
BseLI CCNNNNNNNGG 5 cut(s) 244, 469, 542, 600, 656
BseMI GCAATG 1 cut(s) 433
BseNI ACTGG 4 cut(s) 178, 356, 631, 948
BseRI GAGGAG 1 cut(s) 525
BseXI GCAGC 1 cut(s) 491
BshFI GGCC 2 cut(s) 621, 928
BshTI ACCGGT 1 cut(s) 994
BsiSI CCGG 2 cut(s) 321, 995
BslFI GGGAC 3 cut(s) 115, 586, 854
BslI CCNNNNNNNGG 5 cut(s) 244, 469, 542, 600, 656
BsmFI GGGAC 3 cut(s) 115, 586, 854
BsnI GGCC 2 cut(s) 621, 928
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 1 cut(s) 682
BspANI GGCC 2 cut(s) 621, 928
BspLI GGNNCC 1 cut(s) 201
BspPI GGATC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 994
BsrI ACTGG 4 cut(s) 178, 356, 631, 948
BssAI RCCGGY 1 cut(s) 994
BssECI CCNNGG 2 cut(s) 468, 568
BssMI GATC 1 cut(s) 682
BssT1I CCWWGG 2 cut(s) 468, 568
Bst4CI ACNGT 7 cut(s) 106, 190, 305, 364, 391, 405, 511
Bst6I CTCTTC 2 cut(s) 760, 862
BstAPI GCANNNNNTGC 1 cut(s) 898
BstBAI YACGTR 1 cut(s) 97
BstDEI CTNAG 2 cut(s) 474, 1055
BstENI CCTNNNNNAGG 1 cut(s) 467
BstF5I GGATG 4 cut(s) 221, 721, 749, 894
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 1 cut(s) 685
BstMBI GATC 1 cut(s) 682
BstMWI GCNNNNNNNGC 5 cut(s) 35, 260, 479, 898, 1037
BstSFI CTRYAG 1 cut(s) 1082
BstV1I GCAGC 1 cut(s) 491
BstV2I GAAGAC 1 cut(s) 456
BsuI GTATCC 2 cut(s) 393, 442
BsuRI GGCC 2 cut(s) 621, 928
BtsCI GGATG 4 cut(s) 221, 721, 749, 894
BtsI GCAGTG 2 cut(s) 883, 896
BtsIMutI CAGTG 3 cut(s) 360, 883, 896
CaiI CAGNNNCTG 1 cut(s) 362
CfoI GCGC 1 cut(s) 326
Cfr10I RCCGGY 1 cut(s) 994
Cfr13I GGNCC 5 cut(s) 199, 331, 554, 620, 946
Csp6I GTAC 3 cut(s) 21, 674, 1060
CspAI ACCGGT 1 cut(s) 994
CviAII CATG 3 cut(s) 239, 837, 905
CviQI GTAC 3 cut(s) 21, 674, 1060
DdeI CTNAG 2 cut(s) 474, 1055
DpnI GATC 1 cut(s) 684
DpnII GATC 1 cut(s) 682
DrdI GACNNNNNNGTC 1 cut(s) 552
DseDI GACNNNNNNGTC 1 cut(s) 552
EaeI YGGCCR 1 cut(s) 926
Eam1104I CTCTTC 2 cut(s) 760, 862
EarI CTCTTC 2 cut(s) 760, 862
Eco130I CCWWGG 2 cut(s) 468, 568
Eco24I GRGCYC 1 cut(s) 643
Eco47I GGWCC 4 cut(s) 199, 331, 554, 946
Eco57I CTGAAG 2 cut(s) 465, 549
EcoNI CCTNNNNNAGG 1 cut(s) 467
EcoO109I RGGNCCY 3 cut(s) 199, 554, 620
EcoT14I CCWWGG 2 cut(s) 468, 568
EcoT38I GRGCYC 1 cut(s) 643
ErhI CCWWGG 2 cut(s) 468, 568
FaeI CATG 3 cut(s) 242, 840, 908
FalI AAGNNNNNCTT 4 cut(s) 570, 602, 714, 746
FaqI GGGAC 3 cut(s) 115, 586, 854
FatI CATG 3 cut(s) 238, 836, 904
Fnu4HI GCNGC 1 cut(s) 480
FokI GGATG 4 cut(s) 208, 728, 756, 901
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 1 cut(s) 480
FspBI CTAG 5 cut(s) 18, 50, 524, 864, 1037
GlaI GCGC 1 cut(s) 325
GluI GCNGC 1 cut(s) 480
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 2 cut(s) 621, 928
HapII CCGG 2 cut(s) 321, 995
HhaI GCGC 1 cut(s) 326
Hin1II CATG 3 cut(s) 242, 840, 908
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HindIII AAGCTT 1 cut(s) 882
HinfI GANTC 4 cut(s) 214, 410, 833, 1078
HpaII CCGG 2 cut(s) 321, 995
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 1 cut(s) 992
Hpy188I TCNGA 3 cut(s) 260, 409, 832
Hpy188III TCNNGA 3 cut(s) 299, 587, 644
Hpy8I GTNNAC 1 cut(s) 992
HpyAV CCTTC 3 cut(s) 193, 997, 1033
HpyCH4III ACNGT 7 cut(s) 106, 190, 305, 364, 391, 405, 511
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 6 cut(s) 254, 268, 368, 606, 892, 938
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 260, 479, 898, 1037
HpyF3I CTNAG 2 cut(s) 474, 1055
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 3 cut(s) 242, 840, 908
HspAI GCGC 1 cut(s) 324
Kzo9I GATC 1 cut(s) 682
LmnI GCTCC 2 cut(s) 538, 906
Lsp1109I GCAGC 1 cut(s) 491
LweI GCATC 2 cut(s) 734, 879
MaeI CTAG 5 cut(s) 18, 50, 524, 864, 1037
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 358
MalI GATC 1 cut(s) 684
MboI GATC 1 cut(s) 682
MboII GAAGA 4 cut(s) 458, 461, 777, 879
MfeI CAATTG 1 cut(s) 147
MhlI GDGCHC 1 cut(s) 643
MlsI TGGCCA 1 cut(s) 928
MluCI AATT 6 cut(s) 147, 249, 751, 801, 812, 1009
MluNI TGGCCA 1 cut(s) 928
MlyI GAGTC 2 cut(s) 223, 419
MmeI TCCRAC 3 cut(s) 387, 471, 1030
MnlI CCTC 9 cut(s) 49, 190, 546, 574, 611, 671, 706, 863, 967
Mox20I TGGCCA 1 cut(s) 928
MroXI GAANNNNTTC 1 cut(s) 453
MscI TGGCCA 1 cut(s) 928
MseI TTAA 1 cut(s) 1074
MslI CAYNNNNRTG 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 928
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 2 cut(s) 321, 995
MunI CAATTG 1 cut(s) 147
MwoI GCNNNNNNNGC 5 cut(s) 35, 260, 479, 898, 1037
NdeII GATC 1 cut(s) 682
NlaIII CATG 3 cut(s) 242, 840, 908
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 833
PflMI CCANNNNNTGG 1 cut(s) 244
PinAI ACCGGT 1 cut(s) 994
PkrI GCNGC 1 cut(s) 481
PleI GAGTC 2 cut(s) 222, 418
PpsI GAGTC 2 cut(s) 222, 418
Ppu21I YACGTR 1 cut(s) 97
PpuMI RGGWCCY 2 cut(s) 199, 554
Psp5II RGGWCCY 2 cut(s) 199, 554
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 5 cut(s) 199, 331, 554, 620, 946
PspPPI RGGWCCY 2 cut(s) 199, 554
PstNI CAGNNNCTG 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 3 cut(s) 22, 675, 1061
RsaNI GTAC 3 cut(s) 21, 674, 1060
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 1 cut(s) 1074
SatI GCNGC 1 cut(s) 480
Sau3AI GATC 1 cut(s) 682
Sau96I GGNCC 5 cut(s) 199, 331, 554, 620, 946
ScaI AGTACT 1 cut(s) 22
SchI GAGTC 2 cut(s) 223, 419
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 2 cut(s) 734, 879
SfcI CTRYAG 1 cut(s) 1082
SinI GGWCC 4 cut(s) 199, 331, 554, 946
SmiMI CAYNNNNRTG 1 cut(s) 191
Sse9I AATT 6 cut(s) 147, 249, 751, 801, 812, 1009
SspMI CTAG 5 cut(s) 18, 50, 524, 864, 1037
StyI CCWWGG 2 cut(s) 468, 568
TaaI ACNGT 7 cut(s) 106, 190, 305, 364, 391, 405, 511
TaiI ACGT 1 cut(s) 99
TaqI TCGA 1 cut(s) 212
TasI AATT 6 cut(s) 147, 249, 751, 801, 812, 1009
TatI WGTACW 1 cut(s) 20
TfiI GAWTC 1 cut(s) 833
Tru1I TTAA 1 cut(s) 1074
Tru9I TTAA 1 cut(s) 1074
TscAI CASTG 3 cut(s) 367, 883, 903
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 2 cut(s) 73, 210
TspRI CASTG 3 cut(s) 367, 883, 903
Van91I CCANNNNNTGG 1 cut(s) 244
VpaK11BI GGWCC 4 cut(s) 199, 331, 554, 946
XagI CCTNNNNNAGG 1 cut(s) 467
XapI RAATTY 1 cut(s) 1009
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 5 cut(s) 18, 50, 524, 864, 1037
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.