Rh5BG051400

PAP-specific phosphatase HAL2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002929
Physical Location & Seq
Forward (+)
4090407 .. 4093582
3176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG051400.1

Sequence Viewer

Length: 1368 bp
ATGCCTTTACATTGTTCTAGTATGGCTATAAAAGCCTCTCACAATTTAGGACTTGTGAAATTGAATAACAAGCGCAAGTCCATAATCAACCACGTAGGAACAGTTAGCTTCTCTAAAACCCACCACCACAGAATCTCAAAACCCATTGGCTGCTTATCCAAGCTCAGCCAAACCCAGTCTTTCACAGTAATGGAGGACCCAAAAAGGTTGAGTCTTTCATCCCTACAATCCATGAAGCACACCCAAGAATTGGAAACTGCAGTCAGAGCTGTGCAAATGGCTTGTTCACTCTGCCAGAAAGTTCAGGAGAGTTTGATTTCCAAGTCCAGCGCTGTGGTTCAATCCAAAGGTGACAACTCTCCAGTCACTGTTGCAGATTGGAGTGTCCAAGCAACTGTGAGCTGGATACTGTCGGAGTCTTTTGGGAGTAGCAATGTGTCCATTGTAGCTGAAGAAGACATTCAAAACCTGTCCAAGGCTAAGGCAGGTGGAGTATTGGAAGCAGTAGTAAAAACTGTAAATGATTGTCTAGCTGAAGCACCTCGTTTTGGACTTCAAGGTCCGAAAAAGGCCCTTGGGACTACAGAGGTTCTTGAAGCCATCAGTCGGTGCAACTCAAGGGGAGGCCCTACTGGGAGATTTTGGGCTCTTGACCCTGTTGATGGAACATTGGGTTTTGTACGAGGGGATCAATATGCTATAGCTCTGGCTCTAATAGAGGATGGAGAAGTTGTGCTTGGAGTTCTTGGATGCCCTAATTACCCAATGAGAAAAGAATGGCTAAACTATCATCACCGGTATCATAGAATTATATCCAAATTGACTCCACCAACATCAGAATCATGGGACAAAGGCTGTGTAATATATGCTAGAAGAGGTAGTGGTGAGGCTTGGATGCAACCCTTGCTCCATGTAAACAAGAACTTTTTGTGGCCAAACTCTGCAAAACCTGTCCGAGTGTCCTCCATCGATAATCCATCATTGGCAACCTTTTGTGAACCTGTTGAGAAGGCAAATTCAAGTCATTCCTTCACAGCAGGACTAGCTCACAGTGTTGGACTTAGGAAGCAACCATTAAGAGTATATAGCATGGTGAAATATGCAGCTATAGCTCGTGGGGATGCCGAGATTTTTATGAAGTTTGCAAGGGCTGGTTACAAAGAGAAGATATGGGATCATGCAGCTGGTGTTGTCATCATACAGGAGGCTGGTGGTGTGGTTTCAGACGCCGGGGGGTGTCCACTGGACTTTTCAAAGGGTATATACTTGGAAGGTCTTGATCGAGGTGTAATAGCTAGTGCCGGAGCAAACCTACACGACAAGATCATCAGGGCTGTTGATGCTAGCTGGGACTCCTCTAGTCTGTAG

Protein Analysis

455

Amino Acids

49.31

Weight (kDa)

9.03

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inositol_P PF00459 118 - 449 1.6e-44 Inositol monophosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
AasI GACNNNNNNGTC 1 cut(s) 558
Acc36I ACCTGC 1 cut(s) 476
AccB7I CCANNNNNTGG 1 cut(s) 250
AclWI GGATC 2 cut(s) 696, 1182
AcoI YGGCCR 1 cut(s) 932
AcsI RAATTY 1 cut(s) 1015
AcuI CTGAAG 2 cut(s) 471, 555
AcyI GRCGYC 1 cut(s) 1227
AfaI GTAC 1 cut(s) 681
AfeI AGCGCT 1 cut(s) 331
AfiI CCNNNNNNNGG 5 cut(s) 250, 475, 548, 606, 662
AgeI ACCGGT 1 cut(s) 795
AgsI TTSAA 7 cut(s) 64, 341, 464, 557, 596, 1020, 1254
AjuI GAANNNNNNNTTGG 2 cut(s) 720, 752
AlwI GGATC 2 cut(s) 696, 1182
AlwNI CAGNNNCTG 1 cut(s) 368
Aor51HI AGCGCT 1 cut(s) 331
AoxI GGCC 3 cut(s) 570, 625, 932
ApeKI GCWGC 3 cut(s) 150, 1103, 1181
ApoI RAATTY 1 cut(s) 1015
AsiGI ACCGGT 1 cut(s) 795
Asp700I GAANNNNTTC 1 cut(s) 459
AspLEI GCGC 2 cut(s) 75, 332
AspS9I GGNCC 4 cut(s) 196, 560, 571, 626
AsuC2I CCSGG 1 cut(s) 1231
AsuHPI GGTGA 4 cut(s) 362, 785, 896, 1105
AsuNHI GCTAGC 1 cut(s) 1343
AvaII GGWCC 2 cut(s) 196, 560
BalI TGGCCA 1 cut(s) 934
BanII GRGCYC 1 cut(s) 649
BauI CACGAG 1 cut(s) 1113
BbsI GAAGAC 1 cut(s) 462
BbvI GCAGC 3 cut(s) 137, 1115, 1193
BccI CCATC 5 cut(s) 608, 656, 716, 974, 985
BciVI GTATCC 1 cut(s) 399
BcnI CCSGG 1 cut(s) 1231
BfaI CTAG 7 cut(s) 18, 530, 870, 1043, 1296, 1344, 1359
BfmI CTRYAG 5 cut(s) 258, 582, 699, 1107, 1364
BfoI RGCGCY 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 476
BfuI GTATCC 1 cut(s) 399
BisI GCNGC 3 cut(s) 151, 1104, 1182
BlpI GCTNAGC 1 cut(s) 164
BlsI GCNGC 3 cut(s) 152, 1105, 1183
Bme1390I CCNGG 1 cut(s) 1231
Bme18I GGWCC 2 cut(s) 196, 560
BmgT120I GGNCC 4 cut(s) 196, 560, 571, 626
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 1231
BmrI ACTGGG 2 cut(s) 169, 642
BmsI GCATC 4 cut(s) 740, 885, 1111, 1330
BmtI GCTAGC 1 cut(s) 1347
BmuI ACTGGG 2 cut(s) 169, 642
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 1 cut(s) 345
Bpu10I CCTNAGC 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 601
BpuMI CCSGG 1 cut(s) 1231
Bsa29I ATCGAT 1 cut(s) 969
BsaAI YACGTR 1 cut(s) 94
BsaHI GRCGYC 1 cut(s) 1227
BsaJI CCNNGG 3 cut(s) 474, 574, 1230
BsaWI WCCGGW 1 cut(s) 795
BsaXI ACNNNNNCTCC 4 cut(s) 717, 747, 891, 921
Bsc4I CCNNNNNNNGG 5 cut(s) 250, 475, 548, 606, 662
Bse118I RCCGGY 1 cut(s) 795
Bse1I ACTGG 4 cut(s) 175, 362, 637, 1248
Bse3DI GCAATG 1 cut(s) 439
BseCI ATCGAT 1 cut(s) 969
BseDI CCNNGG 3 cut(s) 474, 574, 1230
BseGI GGATG 5 cut(s) 218, 727, 755, 900, 1126
BseLI CCNNNNNNNGG 5 cut(s) 250, 475, 548, 606, 662
BseMI GCAATG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 178
BseNI ACTGG 4 cut(s) 175, 362, 637, 1248
BseRI GAGGAG 1 cut(s) 1345
BseXI GCAGC 3 cut(s) 137, 1115, 1193
BseYI CCCAGC 1 cut(s) 1347
BshFI GGCC 3 cut(s) 572, 627, 934
BshTI ACCGGT 1 cut(s) 795
BshVI ATCGAT 1 cut(s) 969
BsiSI CCGG 3 cut(s) 796, 1230, 1302
BslFI GGGAC 3 cut(s) 592, 860, 1364
BslI CCNNNNNNNGG 5 cut(s) 250, 475, 548, 606, 662
BsmFI GGGAC 3 cut(s) 592, 860, 1364
BsnI GGCC 3 cut(s) 572, 627, 934
Bsp1286I GDGCHC 1 cut(s) 649
Bsp143I GATC 4 cut(s) 688, 1174, 1279, 1323
Bsp1720I GCTNAGC 1 cut(s) 164
BspANI GGCC 3 cut(s) 572, 627, 934
BspCNI CTCAG 1 cut(s) 177
BspDI ATCGAT 1 cut(s) 969
BspLI GGNNCC 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 262
BspMI ACCTGC 1 cut(s) 476
BspOI GCTAGC 1 cut(s) 1347
BspPI GGATC 2 cut(s) 696, 1182
BsrDI GCAATG 1 cut(s) 439
BsrFI RCCGGY 1 cut(s) 795
BsrI ACTGG 4 cut(s) 175, 362, 637, 1248
BssAI RCCGGY 1 cut(s) 795
BssECI CCNNGG 3 cut(s) 474, 574, 1230
BssMI GATC 4 cut(s) 688, 1174, 1279, 1323
BssNI GRCGYC 1 cut(s) 1227
BssSI CACGAG 1 cut(s) 1113
BssT1I CCWWGG 2 cut(s) 474, 574
Bst2BI CACGAG 1 cut(s) 1113
Bst4CI ACNGT 7 cut(s) 103, 187, 370, 397, 411, 517, 1052
Bst6I CTCTTC 1 cut(s) 868
BstACI GRCGYC 1 cut(s) 1227
BstAPI GCANNNNNTGC 1 cut(s) 904
BstBAI YACGTR 1 cut(s) 94
BstC8I GCNNGC 1 cut(s) 1345
BstDEI CTNAG 3 cut(s) 164, 480, 1061
BstENI CCTNNNNNAGG 1 cut(s) 473
BstF5I GGATG 5 cut(s) 218, 727, 755, 900, 1126
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 2 cut(s) 75, 332
BstKTI GATC 4 cut(s) 691, 1177, 1282, 1326
BstMBI GATC 4 cut(s) 688, 1174, 1279, 1323
BstMWI GCNNNNNNNGC 6 cut(s) 32, 266, 904, 1043, 1109, 1340
BstSCI CCNGG 1 cut(s) 1229
BstSFI CTRYAG 5 cut(s) 258, 582, 699, 1107, 1364
BstV1I GCAGC 3 cut(s) 137, 1115, 1193
BstV2I GAAGAC 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 969
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 3 cut(s) 572, 627, 934
BsuTUI ATCGAT 1 cut(s) 969
BtsCI GGATG 5 cut(s) 218, 727, 755, 900, 1126
BtsIMutI CAGTG 3 cut(s) 366, 1057, 1241
BveI ACCTGC 1 cut(s) 476
Cac8I GCNNGC 1 cut(s) 1345
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 2 cut(s) 75, 332
Cfr10I RCCGGY 1 cut(s) 795
Cfr13I GGNCC 4 cut(s) 196, 560, 571, 626
ClaI ATCGAT 1 cut(s) 969
CseI GACGC 1 cut(s) 1235
Csp6I GTAC 1 cut(s) 680
CspAI ACCGGT 1 cut(s) 795
CviAII CATG 5 cut(s) 232, 843, 911, 1090, 1178
CviQI GTAC 1 cut(s) 680
DdeI CTNAG 3 cut(s) 164, 480, 1061
DpnI GATC 4 cut(s) 690, 1176, 1281, 1325
DpnII GATC 4 cut(s) 688, 1174, 1279, 1323
DrdI GACNNNNNNGTC 1 cut(s) 558
DseDI GACNNNNNNGTC 1 cut(s) 558
EaeI YGGCCR 1 cut(s) 932
Eam1104I CTCTTC 1 cut(s) 868
EarI CTCTTC 1 cut(s) 868
Eco130I CCWWGG 2 cut(s) 474, 574
Eco24I GRGCYC 1 cut(s) 649
Eco47I GGWCC 2 cut(s) 196, 560
Eco47III AGCGCT 1 cut(s) 331
Eco57I CTGAAG 2 cut(s) 471, 555
EcoNI CCTNNNNNAGG 1 cut(s) 473
EcoO109I RGGNCCY 3 cut(s) 196, 571, 626
EcoT14I CCWWGG 2 cut(s) 474, 574
EcoT38I GRGCYC 1 cut(s) 649
ErhI CCWWGG 2 cut(s) 474, 574
FaeI CATG 5 cut(s) 235, 846, 914, 1093, 1181
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 3 cut(s) 592, 860, 1364
FatI CATG 5 cut(s) 231, 842, 910, 1089, 1177
Fnu4HI GCNGC 3 cut(s) 151, 1104, 1182
FokI GGATG 5 cut(s) 205, 734, 762, 907, 1133
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 3 cut(s) 151, 1104, 1182
FspBI CTAG 7 cut(s) 18, 530, 870, 1043, 1296, 1344, 1359
GlaI GCGC 2 cut(s) 74, 331
GluI GCNGC 3 cut(s) 151, 1104, 1182
GsaI CCCAGC 1 cut(s) 1351
GsuI CTGGAG 1 cut(s) 345
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 3 cut(s) 572, 627, 934
HapII CCGG 3 cut(s) 796, 1230, 1302
HgaI GACGC 1 cut(s) 1235
HhaI GCGC 2 cut(s) 75, 332
Hin1I GRCGYC 1 cut(s) 1227
Hin1II CATG 5 cut(s) 235, 846, 914, 1093, 1181
Hin6I GCGC 2 cut(s) 73, 330
HinP1I GCGC 2 cut(s) 73, 330
HinfI GANTC 6 cut(s) 132, 211, 416, 823, 839, 1352
HpaII CCGG 3 cut(s) 796, 1230, 1302
HphI GGTGA 4 cut(s) 362, 785, 896, 1105
Hpy166II GTNNAC 4 cut(s) 287, 916, 998, 1241
Hpy188I TCNGA 6 cut(s) 266, 415, 564, 838, 956, 1225
Hpy188III TCNNGA 4 cut(s) 305, 593, 650, 1277
Hpy8I GTNNAC 4 cut(s) 287, 916, 998, 1241
HpyAV CCTTC 3 cut(s) 1003, 1039, 1265
HpyCH4III ACNGT 7 cut(s) 103, 187, 370, 397, 411, 517, 1052
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 9 cut(s) 260, 274, 374, 612, 898, 944, 1103, 1145, 1181
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 266, 904, 1043, 1109, 1340
HpyF3I CTNAG 3 cut(s) 164, 480, 1061
HpySE526I ACGT 1 cut(s) 93
Hsp92I GRCGYC 1 cut(s) 1227
Hsp92II CATG 5 cut(s) 235, 846, 914, 1093, 1181
HspAI GCGC 2 cut(s) 73, 330
Kzo9I GATC 4 cut(s) 688, 1174, 1279, 1323
LmnI GCTCC 2 cut(s) 912, 1304
Lsp1109I GCAGC 3 cut(s) 137, 1115, 1193
LweI GCATC 4 cut(s) 740, 885, 1111, 1330
MaeI CTAG 7 cut(s) 18, 530, 870, 1043, 1296, 1344, 1359
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 3 cut(s) 350, 364, 1154
MalI GATC 4 cut(s) 690, 1176, 1281, 1325
MboI GATC 4 cut(s) 688, 1174, 1279, 1323
MboII GAAGA 4 cut(s) 464, 467, 885, 1177
MhlI GDGCHC 1 cut(s) 649
MlsI TGGCCA 1 cut(s) 934
MluCI AATT 7 cut(s) 43, 59, 248, 757, 807, 818, 1015
MluNI TGGCCA 1 cut(s) 934
MlyI GAGTC 4 cut(s) 220, 425, 817, 1346
MmeI TCCRAC 2 cut(s) 393, 1036
Mox20I TGGCCA 1 cut(s) 934
MroXI GAANNNNTTC 1 cut(s) 459
MscI TGGCCA 1 cut(s) 934
MseI TTAA 1 cut(s) 1076
MslI CAYNNNNRTG 1 cut(s) 188
Msp20I TGGCCA 1 cut(s) 934
MspA1I CMGCKG 1 cut(s) 1184
MspI CCGG 3 cut(s) 796, 1230, 1302
MspR9I CCNGG 1 cut(s) 1231
MwoI GCNNNNNNNGC 6 cut(s) 32, 266, 904, 1043, 1109, 1340
NciI CCSGG 1 cut(s) 1231
NdeII GATC 4 cut(s) 688, 1174, 1279, 1323
NheI GCTAGC 1 cut(s) 1343
NlaIII CATG 5 cut(s) 235, 846, 914, 1093, 1181
NlaIV GGNNCC 1 cut(s) 198
NmeAIII GCCGAG 1 cut(s) 1150
NmuCI GTSAC 2 cut(s) 350, 364
PaqCI CACCTGC 1 cut(s) 476
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 132, 839
PflMI CCANNNNNTGG 1 cut(s) 250
PinAI ACCGGT 1 cut(s) 795
PkrI GCNGC 3 cut(s) 152, 1105, 1183
PleI GAGTC 4 cut(s) 219, 424, 817, 1346
PpsI GAGTC 4 cut(s) 219, 424, 817, 1346
Ppu21I YACGTR 1 cut(s) 94
PpuMI RGGWCCY 1 cut(s) 196
Psp5II RGGWCCY 1 cut(s) 196
PspFI CCCAGC 1 cut(s) 1347
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 4 cut(s) 196, 560, 571, 626
PspPPI RGGWCCY 1 cut(s) 196
PstI CTGCAG 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 368
PvuII CAGCTG 1 cut(s) 1184
RsaI GTAC 1 cut(s) 681
RsaNI GTAC 1 cut(s) 680
RseI CAYNNNNRTG 1 cut(s) 188
SaqAI TTAA 1 cut(s) 1076
SatI GCNGC 3 cut(s) 151, 1104, 1182
Sau3AI GATC 4 cut(s) 688, 1174, 1279, 1323
Sau96I GGNCC 4 cut(s) 196, 560, 571, 626
SchI GAGTC 4 cut(s) 220, 425, 817, 1346
ScrFI CCNGG 1 cut(s) 1231
SduI GDGCHC 1 cut(s) 649
SfaNI GCATC 4 cut(s) 740, 885, 1111, 1330
SfcI CTRYAG 5 cut(s) 258, 582, 699, 1107, 1364
SinI GGWCC 2 cut(s) 196, 560
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
Sse9I AATT 7 cut(s) 43, 59, 248, 757, 807, 818, 1015
SspMI CTAG 7 cut(s) 18, 530, 870, 1043, 1296, 1344, 1359
StyD4I CCNGG 1 cut(s) 1229
StyI CCWWGG 2 cut(s) 474, 574
TaaI ACNGT 7 cut(s) 103, 187, 370, 397, 411, 517, 1052
TaiI ACGT 1 cut(s) 96
TaqI TCGA 2 cut(s) 969, 1282
TasI AATT 7 cut(s) 43, 59, 248, 757, 807, 818, 1015
TfiI GAWTC 2 cut(s) 132, 839
Tru1I TTAA 1 cut(s) 1076
Tru9I TTAA 1 cut(s) 1076
TscAI CASTG 3 cut(s) 373, 1057, 1248
TseFI GTSAC 2 cut(s) 350, 364
TseI GCWGC 3 cut(s) 150, 1103, 1181
Tsp45I GTSAC 2 cut(s) 350, 364
TspDTI ATGAA 3 cut(s) 207, 248, 1151
TspRI CASTG 3 cut(s) 373, 1057, 1248
Van91I CCANNNNNTGG 1 cut(s) 250
VpaK11BI GGWCC 2 cut(s) 196, 560
XagI CCTNNNNNAGG 1 cut(s) 473
XapI RAATTY 1 cut(s) 1015
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 7 cut(s) 18, 530, 870, 1043, 1296, 1344, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.