pycom10g25400

PAP-specific phosphatase HAL2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002929
Physical Location & Seq
Reverse (-)
27052733 .. 27055913
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25400.1

Sequence Viewer

Length: 1326 bp
ATGGCTATAAAATTCTCGCACAATTTGGGTCTTGAAAAATGGAATGACAAGAAGTTGACAAGCAGGTACAAAACCCACCTCAACTTTTCTGGGAAATTTGGCTGCTGCTTATCCAAGTTCAGGAAAACCCAATCCTTCTCAGTAATGGAGGGCCTGAAAAGTTCGCGTCTTTTGGCTCCGGAGCCAGTGAAAAATTACTCCCAGGAATTGGATGTGGCTGTTAGAGCTGTGCAATTGGCTTGCTCTCTCTGCCAGAGAGTTCAGAACAGTTTGATTTCGAAGAACAGCGATGGGGTCCAATCCAAAGATGATAACTCTCCTGTCACTGTGGCAGATTGGAGTGTCCAAGCAACTGTGAGCTGGATACTGTCCGAGTCTTTTGGGAGCAGCAACGTGTCCATTATTGCTGAAGAAGATATTCAAAATTTGTCCATGGCAAAGGCAGGTGGAGTACTAAAAGATGTAGTGAAAACTGTAAACGAATGTCTAGCTGAAGCACCTCGTTTTGGACTTAAAAGTCCGAAAATGGCCCTTGGGACTACAGAGGTTCTTGAAGCCATCAGTCGGTGCAACTCACTTGGAGGCACGGCTGGGAGATTTTGGACACTTGACCCCGTTGATGGCACATTGGGTTTTGTCCGCGGTGATCAATATACAATAGCTCTGGCTTTAATAGAGGATGGAGAAGTTGTCCTTGGAGTTCTTGGCTGTCCCAATTACCCAATGAGAAAGGAATGGCTCAATTATCATCACCGGTATCATAGAATTATATCTAAATTGACACCACCAACGTCGGAATCTTGGGACAAAGGCTGTGTGATATATGCTAGAAGAGGCAGCGGTGAGGCTTGGATGCAACCGCTGCTCCATGTAAATAAGAACTTGGTGTGGCCAAACTCTGCAAAACCTGTCCGAGTATCCTCCATAGATAATCCAGCACTGGCAACATTTTGTGAACCTGTTGAGAAGGCAAATTCAAGTCATTCCTTCACAGCAGGGCTAGCTCATAGTGTCGGGCTTAGGAAGCAGCCACTGCGAGTATACAGCATGGTGAAGTACGCAGCCATAGCTCGTGGAGATGCCGAGATTTTTATGAAGTTTGCAAGGGCTGGCTACAAGGAGAAGATTTGGGATCATGCAGCAGGCGTTGTTATCATACAAGAGGCTGGTGGGGTTGTTACAGATGCCGGAGGGCGTCCACTGGACTTTTCGAAGGGCATATACTTGGAAGGCCTTGATCGATGTGTAGTTGCTACTGCTGGAGCCAATCTACATGACAAGATCATCAGGGCCGTTGATGCTAGCTGGGACTCCTCCAGTCTATAA

Protein Analysis

442

Amino Acids

48.21

Weight (kDa)

8.86

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inositol_P PF00459 103 - 434 2.3e-42 Inositol monophosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 434
AasI GACNNNNNNGTC 1 cut(s) 516
Acc36I ACCTGC 2 cut(s) 54, 434
AccB7I CCANNNNNTGG 1 cut(s) 208
AccI GTMKAC 1 cut(s) 1041
AccII CGCG 2 cut(s) 166, 642
AccIII TCCGGA 1 cut(s) 178
AciI CCGC 4 cut(s) 640, 642, 840, 860
AclWI GGATC 1 cut(s) 1140
AcoI YGGCCR 1 cut(s) 890
AcsI RAATTY 4 cut(s) 11, 95, 424, 973
AcuI CTGAAG 2 cut(s) 429, 513
AcyI GRCGYC 1 cut(s) 1195
AfaI GTAC 3 cut(s) 68, 453, 1058
AfiI CCNNNNNNNGG 5 cut(s) 120, 208, 506, 564, 620
AflIII ACRYGT 1 cut(s) 393
AgeI ACCGGT 1 cut(s) 753
AgsI TTSAA 4 cut(s) 35, 422, 554, 978
AjnI CCWGG 1 cut(s) 201
AjuI GAANNNNNNNTTGG 2 cut(s) 678, 710
AluBI AGCT 7 cut(s) 227, 360, 491, 662, 1004, 1070, 1305
AluI AGCT 7 cut(s) 227, 360, 491, 662, 1004, 1070, 1305
AlwI GGATC 1 cut(s) 1140
AlwNI CAGNNNCTG 1 cut(s) 1033
Aor13HI TCCGGA 1 cut(s) 178
AoxI GGCC 5 cut(s) 151, 528, 890, 1231, 1290
ApeKI GCWGC 8 cut(s) 102, 105, 387, 837, 862, 1027, 1061, 1139
ApoI RAATTY 4 cut(s) 11, 95, 424, 973
AsiGI ACCGGT 1 cut(s) 753
Asp700I GAANNNNTTC 1 cut(s) 417
AspS9I GGNCC 4 cut(s) 151, 295, 529, 1290
AsuHPI GGTGA 4 cut(s) 656, 743, 854, 1063
AsuII TTCGAA 2 cut(s) 278, 1211
AsuNHI GCTAGC 2 cut(s) 1000, 1301
AvaII GGWCC 1 cut(s) 295
BalI TGGCCA 1 cut(s) 892
BarI GAAGNNNNNNTAC 2 cut(s) 1205, 1237
BauI CACGAG 1 cut(s) 1071
BbvI GCAGC 8 cut(s) 89, 92, 399, 849, 849, 1039, 1073, 1151
BccI CCATC 4 cut(s) 284, 566, 614, 674
BceAI ACGGC 2 cut(s) 603, 1277
BciT130I CCWGG 1 cut(s) 203
BciVI GTATCC 2 cut(s) 357, 928
BclI TGATCA 1 cut(s) 646
BfaI CTAG 4 cut(s) 488, 828, 1001, 1302
BfmI CTRYAG 1 cut(s) 540
BfuAI ACCTGC 2 cut(s) 54, 434
BfuI GTATCC 2 cut(s) 357, 928
BisI GCNGC 8 cut(s) 103, 106, 388, 838, 863, 1028, 1062, 1140
BlsI GCNGC 8 cut(s) 104, 107, 389, 839, 864, 1029, 1063, 1141
BmcAI AGTACT 1 cut(s) 453
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 4 cut(s) 151, 295, 529, 1290
BmiI GGNNCC 4 cut(s) 177, 183, 296, 1264
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 4 cut(s) 843, 1069, 1174, 1288
BmtI GCTAGC 2 cut(s) 1004, 1305
BpmI CTGGAG 2 cut(s) 1281, 1300
Bpu10I CCTNAGC 1 cut(s) 1019
Bpu14I TTCGAA 2 cut(s) 278, 1211
Bsa29I ATCGAT 1 cut(s) 1240
BsaHI GRCGYC 1 cut(s) 1195
BsaJI CCNNGG 5 cut(s) 201, 432, 532, 640, 694
BsaWI WCCGGW 2 cut(s) 178, 753
BsaXI ACNNNNNCTCC 4 cut(s) 675, 705, 849, 879
Bsc4I CCNNNNNNNGG 5 cut(s) 120, 208, 506, 564, 620
Bse118I RCCGGY 1 cut(s) 753
Bse1I ACTGG 4 cut(s) 185, 945, 1206, 1317
BseAI TCCGGA 1 cut(s) 178
BseBI CCWGG 1 cut(s) 203
BseCI ATCGAT 1 cut(s) 1240
BseDI CCNNGG 5 cut(s) 201, 432, 532, 640, 694
BseGI GGATG 3 cut(s) 217, 685, 858
BseLI CCNNNNNNNGG 5 cut(s) 120, 208, 506, 564, 620
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 4 cut(s) 185, 945, 1206, 1317
BseRI GAGGAG 1 cut(s) 1303
BseXI GCAGC 8 cut(s) 89, 92, 399, 849, 849, 1039, 1073, 1151
BseYI CCCAGC 2 cut(s) 590, 1305
Bsh1236I CGCG 2 cut(s) 166, 642
BshFI GGCC 5 cut(s) 153, 530, 892, 1233, 1292
BshTI ACCGGT 1 cut(s) 753
BshVI ATCGAT 1 cut(s) 1240
BsiSI CCGG 3 cut(s) 179, 754, 1188
BslFI GGGAC 4 cut(s) 550, 696, 818, 1322
BslI CCNNNNNNNGG 5 cut(s) 120, 208, 506, 564, 620
BsmFI GGGAC 4 cut(s) 550, 696, 818, 1322
BsnI GGCC 5 cut(s) 153, 530, 892, 1233, 1292
Bsp119I TTCGAA 2 cut(s) 278, 1211
Bsp13I TCCGGA 1 cut(s) 178
Bsp143I GATC 4 cut(s) 646, 1132, 1237, 1281
Bsp19I CCATGG 1 cut(s) 432
BspACI CCGC 4 cut(s) 640, 642, 840, 860
BspANI GGCC 5 cut(s) 153, 530, 892, 1233, 1292
BspCNI CTCAG 1 cut(s) 152
BspDI ATCGAT 1 cut(s) 1240
BspEI TCCGGA 1 cut(s) 178
BspFNI CGCG 2 cut(s) 166, 642
BspLI GGNNCC 4 cut(s) 177, 183, 296, 1264
BspMI ACCTGC 2 cut(s) 54, 434
BspOI GCTAGC 2 cut(s) 1004, 1305
BspPI GGATC 1 cut(s) 1140
BspT104I TTCGAA 2 cut(s) 278, 1211
BsrFI RCCGGY 1 cut(s) 753
BsrI ACTGG 4 cut(s) 185, 945, 1206, 1317
BssAI RCCGGY 1 cut(s) 753
BssECI CCNNGG 5 cut(s) 201, 432, 532, 640, 694
BssMI GATC 4 cut(s) 646, 1132, 1237, 1281
BssNAI GTATAC 1 cut(s) 1042
BssNI GRCGYC 1 cut(s) 1195
BssSI CACGAG 1 cut(s) 1071
BssT1I CCWWGG 3 cut(s) 432, 532, 694
Bst1107I GTATAC 1 cut(s) 1042
Bst2BI CACGAG 1 cut(s) 1071
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 5 cut(s) 269, 328, 355, 369, 475
Bst6I CTCTTC 1 cut(s) 826
BstACI GRCGYC 1 cut(s) 1195
BstAPI GCANNNNNTGC 2 cut(s) 862, 1033
BstBI TTCGAA 2 cut(s) 278, 1211
BstC8I GCNNGC 5 cut(s) 241, 1002, 1111, 1144, 1303
BstDEI CTNAG 2 cut(s) 139, 1019
BstDSI CCRYGG 2 cut(s) 432, 640
BstF5I GGATG 3 cut(s) 217, 685, 858
BstFNI CGCG 2 cut(s) 166, 642
BstKTI GATC 4 cut(s) 649, 1135, 1240, 1284
BstMBI GATC 4 cut(s) 646, 1132, 1237, 1281
BstMWI GCNNNNNNNGC 8 cut(s) 224, 249, 862, 1001, 1024, 1033, 1067, 1298
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 1 cut(s) 540
BstUI CGCG 2 cut(s) 166, 642
BstV1I GCAGC 8 cut(s) 89, 92, 399, 849, 849, 1039, 1073, 1151
BstZ17I GTATAC 1 cut(s) 1042
Bsu15I ATCGAT 1 cut(s) 1240
BsuI GTATCC 2 cut(s) 357, 928
BsuRI GGCC 5 cut(s) 153, 530, 892, 1233, 1292
BsuTUI ATCGAT 1 cut(s) 1240
BtgI CCRYGG 2 cut(s) 432, 640
BtgZI GCGATG 1 cut(s) 303
BtsCI GGATG 3 cut(s) 217, 685, 858
BtsI GCAGTG 1 cut(s) 1031
BtsIMutI CAGTG 5 cut(s) 192, 324, 938, 1031, 1199
BveI ACCTGC 2 cut(s) 54, 434
Cac8I GCNNGC 5 cut(s) 241, 1002, 1111, 1144, 1303
CaiI CAGNNNCTG 1 cut(s) 1033
Cfr10I RCCGGY 1 cut(s) 753
Cfr13I GGNCC 4 cut(s) 151, 295, 529, 1290
Cfr42I CCGCGG 1 cut(s) 643
ClaI ATCGAT 1 cut(s) 1240
CseI GACGC 2 cut(s) 155, 1184
Csp6I GTAC 3 cut(s) 67, 452, 1057
CspAI ACCGGT 1 cut(s) 753
CviAII CATG 5 cut(s) 433, 869, 1048, 1136, 1274
CviQI GTAC 3 cut(s) 67, 452, 1057
DdeI CTNAG 2 cut(s) 139, 1019
DpnI GATC 4 cut(s) 648, 1134, 1239, 1283
DpnII GATC 4 cut(s) 646, 1132, 1237, 1281
DrdI GACNNNNNNGTC 1 cut(s) 516
DseDI GACNNNNNNGTC 1 cut(s) 516
EaeI YGGCCR 1 cut(s) 890
Eam1104I CTCTTC 1 cut(s) 826
EarI CTCTTC 1 cut(s) 826
Eco130I CCWWGG 3 cut(s) 432, 532, 694
Eco147I AGGCCT 1 cut(s) 1233
Eco47I GGWCC 1 cut(s) 295
Eco57I CTGAAG 2 cut(s) 429, 513
EcoO109I RGGNCCY 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 3 cut(s) 432, 532, 694
ErhI CCWWGG 3 cut(s) 432, 532, 694
FaeI CATG 5 cut(s) 436, 872, 1051, 1139, 1277
FalI AAGNNNNNCTT 2 cut(s) 678, 710
FaqI GGGAC 4 cut(s) 550, 696, 818, 1322
FatI CATG 5 cut(s) 432, 868, 1047, 1135, 1273
FbaI TGATCA 1 cut(s) 646
FblI GTMKAC 1 cut(s) 1041
Fnu4HI GCNGC 8 cut(s) 103, 106, 388, 838, 863, 1028, 1062, 1140
FokI GGATG 3 cut(s) 224, 692, 865
Fsp4HI GCNGC 8 cut(s) 103, 106, 388, 838, 863, 1028, 1062, 1140
FspBI CTAG 4 cut(s) 488, 828, 1001, 1302
GluI GCNGC 8 cut(s) 103, 106, 388, 838, 863, 1028, 1062, 1140
GsaI CCCAGC 2 cut(s) 594, 1309
GsuI CTGGAG 2 cut(s) 1281, 1300
HaeIII GGCC 5 cut(s) 153, 530, 892, 1233, 1292
HapII CCGG 3 cut(s) 179, 754, 1188
HgaI GACGC 2 cut(s) 155, 1184
Hin1I GRCGYC 1 cut(s) 1195
Hin1II CATG 5 cut(s) 436, 872, 1051, 1139, 1277
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 3 cut(s) 374, 797, 1310
HpaII CCGG 3 cut(s) 179, 754, 1188
HphI GGTGA 4 cut(s) 656, 743, 854, 1063
Hpy166II GTNNAC 5 cut(s) 57, 478, 956, 1042, 1199
Hpy188I TCNGA 5 cut(s) 264, 373, 522, 796, 914
Hpy188III TCNNGA 4 cut(s) 32, 121, 179, 551
Hpy8I GTNNAC 5 cut(s) 57, 478, 956, 1042, 1199
Hpy99I CGWCG 1 cut(s) 796
HpyAV CCTTC 5 cut(s) 145, 961, 997, 1207, 1223
HpyCH4III ACNGT 5 cut(s) 269, 328, 355, 369, 475
HpyCH4IV ACGT 2 cut(s) 393, 791
HpyCH4V TGCA 6 cut(s) 232, 570, 856, 902, 1103, 1139
HpyF10VI GCNNNNNNNGC 8 cut(s) 224, 249, 862, 1001, 1024, 1033, 1067, 1298
HpyF3I CTNAG 2 cut(s) 139, 1019
HpySE526I ACGT 2 cut(s) 393, 791
Hsp92I GRCGYC 1 cut(s) 1195
Hsp92II CATG 5 cut(s) 436, 872, 1051, 1139, 1277
Kpn2I TCCGGA 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 646
KspI CCGCGG 1 cut(s) 643
Kzo9I GATC 4 cut(s) 646, 1132, 1237, 1281
LmnI GCTCC 5 cut(s) 181, 181, 384, 870, 1262
Lsp1109I GCAGC 8 cut(s) 89, 92, 399, 849, 849, 1039, 1073, 1151
LweI GCATC 4 cut(s) 843, 1069, 1174, 1288
MaeI CTAG 4 cut(s) 488, 828, 1001, 1302
MaeII ACGT 2 cut(s) 393, 791
MaeIII GTNAC 2 cut(s) 322, 1177
MalI GATC 4 cut(s) 648, 1134, 1239, 1283
MboI GATC 4 cut(s) 646, 1132, 1237, 1281
MboII GAAGA 5 cut(s) 292, 422, 425, 843, 1135
MfeI CAATTG 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 892
MluNI TGGCCA 1 cut(s) 892
MlyI GAGTC 2 cut(s) 383, 1304
MmeI TCCRAC 1 cut(s) 774
Mox20I TGGCCA 1 cut(s) 892
MroI TCCGGA 1 cut(s) 178
MroXI GAANNNNTTC 1 cut(s) 417
MscI TGGCCA 1 cut(s) 892
MseI TTAA 2 cut(s) 513, 671
Msp20I TGGCCA 1 cut(s) 892
MspA1I CMGCKG 3 cut(s) 642, 840, 862
MspI CCGG 3 cut(s) 179, 754, 1188
MspR9I CCNGG 1 cut(s) 203
MunI CAATTG 1 cut(s) 233
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 2 cut(s) 166, 642
MwoI GCNNNNNNNGC 8 cut(s) 224, 249, 862, 1001, 1024, 1033, 1067, 1298
NcoI CCATGG 1 cut(s) 432
NdeII GATC 4 cut(s) 646, 1132, 1237, 1281
NheI GCTAGC 2 cut(s) 1000, 1301
NlaIII CATG 5 cut(s) 436, 872, 1051, 1139, 1277
NlaIV GGNNCC 4 cut(s) 177, 183, 296, 1264
NmeAIII GCCGAG 1 cut(s) 1108
NmuCI GTSAC 1 cut(s) 322
NspV TTCGAA 2 cut(s) 278, 1211
PaqCI CACCTGC 1 cut(s) 434
PceI AGGCCT 1 cut(s) 1233
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 1 cut(s) 797
PflMI CCANNNNNTGG 1 cut(s) 208
PinAI ACCGGT 1 cut(s) 753
PkrI GCNGC 8 cut(s) 104, 107, 389, 839, 864, 1029, 1063, 1141
PleI GAGTC 2 cut(s) 382, 1304
PpsI GAGTC 2 cut(s) 382, 1304
Psp6I CCWGG 1 cut(s) 201
PspFI CCCAGC 2 cut(s) 590, 1305
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 4 cut(s) 177, 183, 296, 1264
PspPI GGNCC 4 cut(s) 151, 295, 529, 1290
PstNI CAGNNNCTG 1 cut(s) 1033
RsaI GTAC 3 cut(s) 68, 453, 1058
RsaNI GTAC 3 cut(s) 67, 452, 1057
SacII CCGCGG 1 cut(s) 643
SaqAI TTAA 2 cut(s) 513, 671
SatI GCNGC 8 cut(s) 103, 106, 388, 838, 863, 1028, 1062, 1140
Sau3AI GATC 4 cut(s) 646, 1132, 1237, 1281
Sau96I GGNCC 4 cut(s) 151, 295, 529, 1290
ScaI AGTACT 1 cut(s) 453
SchI GAGTC 2 cut(s) 383, 1304
ScrFI CCNGG 1 cut(s) 203
SfaNI GCATC 4 cut(s) 843, 1069, 1174, 1288
SfcI CTRYAG 1 cut(s) 540
Sfr303I CCGCGG 1 cut(s) 643
SfuI TTCGAA 2 cut(s) 278, 1211
SgrBI CCGCGG 1 cut(s) 643
SinI GGWCC 1 cut(s) 295
SseBI AGGCCT 1 cut(s) 1233
SsiI CCGC 4 cut(s) 640, 642, 840, 860
SspMI CTAG 4 cut(s) 488, 828, 1001, 1302
StuI AGGCCT 1 cut(s) 1233
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 3 cut(s) 432, 532, 694
TaaI ACNGT 5 cut(s) 269, 328, 355, 369, 475
TaiI ACGT 2 cut(s) 396, 794
TaqI TCGA 3 cut(s) 278, 1211, 1240
TatI WGTACW 1 cut(s) 451
TfiI GAWTC 1 cut(s) 797
Tru1I TTAA 2 cut(s) 513, 671
Tru9I TTAA 2 cut(s) 513, 671
TscAI CASTG 5 cut(s) 192, 331, 945, 1038, 1206
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 8 cut(s) 102, 105, 387, 837, 862, 1027, 1061, 1139
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 1 cut(s) 1109
TspRI CASTG 5 cut(s) 192, 331, 945, 1038, 1206
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 295
XapI RAATTY 4 cut(s) 11, 95, 424, 973
XmiI GTMKAC 1 cut(s) 1041
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 4 cut(s) 488, 828, 1001, 1302
ZrmI AGTACT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.