Rroxscaffold_1G00045390

PAP-specific phosphatase HAL2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002929
Physical Location & Seq
Forward (+)
64365336 .. 64368757
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00045390.1

Sequence Viewer

Length: 1104 bp
ATGGAGGACGATAAGTACGCTAAGGAGTTGGACGTGGCCGTTAGAGTGGTGCACATGGCTTGTGCTCTGTGTCAGAGAGTTCAAGAGGGTTTGGTTTCAGCTAGCACTGACCAGGTGAAGTCCAAAGACGATGATTCCCCTGTAACAATTGCAGATTGGAGTGTCCAAGCAACTGTTAGCTGGATACTATCTGAATTCTTTGGGAGTCAAAATGTATCCATTATTGCTGAAGAAGATGTACAAACTCTCTCTAAGGCTGACTCAGTAGGTTTGCTGCAAGCTGTTGTGAATACTGTGAATGAATGCTTAGCTGGAGCACCCAAGTATGGTCTTAAAGGTCCATCAAAAGCCCTCACGACATCTCAAATTCTTGAGGCTATCAGCCGATGCAACTCAGTAGGAGGCCCCAAAGGAAGGCACTGGGTACTTGATCCTGTTGATGGAACATTAGGGTTTGTGCGTGGGGATCAATATGCTGTAGCTCTAGCCCTGATTGAGGATGGAAAAGCTGTTATTGGGGTACTAGGGTGCCCTAATTACCCAATGAAAAAGGAATTGCTCAATTATCATTATCAGTACCATGAAGCCATGTCAAAGTCGTCTCCACCTTCTCCTGATATATGGGAAAGAGGTTGTGTGATGTATGCGAGAAAAGACAGTGGGGAGGCATGGATGCAGCCACCCATCCATGGAGATAAGAAGTTTGAGTGGCCAAATTCTGCTAAACTTATTCGGGTTTCTTCCATCGATGACCCAGCACTGGCTACTAGTTGTGAACCTGTGGAGAAAGCAAATTCAAACCACTCCTTCACAGAAGAGCTTGCTCACAGCGTAGGGCTAAGCCATAGCTCGGGAGATGCTGAGCTGTTCATGAAGTTTGCAAGGACAGGGTACAAGGAGAAGATATGGGATCATGCTGCTGGTGTTGTCATTGTAGAAGAGGCTGGGGGTGTGGTCACTGATGCTGGAGGGCGGCCCCTGGATTTTTCAAAGGGACTGTACTTGGAAGGCCTTGATAGAGGAATAATTGTTTGTTCTGGGGCCACACTGCATGAGAAAATCATTGATGCTGTTTATGCCAGCTGGGACTCTTCTAATTTATGA

Protein Analysis

367

Amino Acids

39.71

Weight (kDa)

5.06

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inositol_P PF00459 45 - 359 5.3e-39 Inositol monophosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 528
AciI CCGC 1 cut(s) 973
AclWI GGATC 3 cut(s) 425, 474, 918
AcoI YGGCCR 2 cut(s) 36, 710
AcsI RAATTY 4 cut(s) 194, 366, 715, 793
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 7 cut(s) 17, 240, 426, 522, 578, 893, 1001
AfiI CCNNNNNNNGG 7 cut(s) 326, 414, 440, 496, 689, 760, 850
AgsI TTSAA 3 cut(s) 83, 798, 990
AhlI ACTAGT 1 cut(s) 767
AjiI CACGTC 1 cut(s) 34
AjnI CCWGG 2 cut(s) 111, 978
Alw21I GWGCWC 3 cut(s) 54, 67, 319
Alw26I GTCTC 1 cut(s) 606
Alw44I GTGCAC 1 cut(s) 50
AlwI GGATC 3 cut(s) 425, 474, 918
Ama87I CYCGRG 1 cut(s) 850
AoxI GGCC 6 cut(s) 36, 403, 710, 974, 1009, 1041
ApaLI GTGCAC 1 cut(s) 50
ApeKI GCWGC 3 cut(s) 274, 676, 917
ApoI RAATTY 4 cut(s) 194, 366, 715, 793
AspS9I GGNCC 4 cut(s) 338, 404, 975, 1041
AsuHPI GGTGA 1 cut(s) 127
AsuNHI GCTAGC 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 850
AvaII GGWCC 1 cut(s) 338
BaeGI GKGCMC 2 cut(s) 54, 533
BaeI ACNNNNGTAYC 2 cut(s) 512, 545
BalI TGGCCA 1 cut(s) 712
BanI GGYRCC 1 cut(s) 528
BarI GAAGNNNNNNTAC 2 cut(s) 222, 254
Bbv12I GWGCWC 3 cut(s) 54, 67, 319
BbvI GCAGC 3 cut(s) 261, 688, 904
BccI CCATC 5 cut(s) 349, 434, 494, 692, 752
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 2 cut(s) 113, 980
BciVI GTATCC 2 cut(s) 177, 226
BcoDI GTCTC 1 cut(s) 606
BcuI ACTAGT 1 cut(s) 767
BfaI CTAG 4 cut(s) 102, 485, 524, 768
BfmI CTRYAG 1 cut(s) 477
BfuI GTATCC 2 cut(s) 177, 226
BisI GCNGC 4 cut(s) 275, 677, 918, 974
BlpI GCTNAGC 3 cut(s) 307, 839, 861
BlsI GCNGC 4 cut(s) 276, 678, 919, 975
Bme1390I CCNGG 2 cut(s) 113, 980
Bme18I GGWCC 1 cut(s) 338
BmeT110I CYCGRG 1 cut(s) 850
BmgBI CACGTC 1 cut(s) 34
BmgT120I GGNCC 4 cut(s) 338, 404, 975, 1041
BmiI GGNNCC 4 cut(s) 406, 530, 977, 1042
BmrFI CCNGG 2 cut(s) 113, 980
BmrI ACTGGG 1 cut(s) 430
BmsI GCATC 5 cut(s) 377, 663, 847, 952, 1057
BmtI GCTAGC 1 cut(s) 105
BmuI ACTGGG 1 cut(s) 430
BpmI CTGGAG 2 cut(s) 333, 987
Bpu10I CCTNAGC 1 cut(s) 21
Bpu1102I GCTNAGC 3 cut(s) 307, 839, 861
BpuEI CTTGAG 1 cut(s) 392
Bsa29I ATCGAT 1 cut(s) 747
BsaJI CCNNGG 2 cut(s) 688, 978
Bsc4I CCNNNNNNNGG 7 cut(s) 326, 414, 440, 496, 689, 760, 850
Bse1I ACTGG 2 cut(s) 425, 765
BseBI CCWGG 2 cut(s) 113, 980
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 2 cut(s) 688, 978
BseGI GGATG 3 cut(s) 505, 678, 684
BseLI CCNNNNNNNGG 7 cut(s) 326, 414, 440, 496, 689, 760, 850
BseMII CTCAG 3 cut(s) 276, 408, 852
BseNI ACTGG 2 cut(s) 425, 765
BseSI GKGCMC 2 cut(s) 54, 533
BseXI GCAGC 3 cut(s) 261, 688, 904
BseYI CCCAGC 3 cut(s) 754, 944, 1083
BshFI GGCC 6 cut(s) 38, 405, 712, 976, 1011, 1043
BshNI GGYRCC 1 cut(s) 528
BshVI ATCGAT 1 cut(s) 747
BsiHKAI GWGCWC 3 cut(s) 54, 67, 319
BsiHKCI CYCGRG 1 cut(s) 850
BslFI GGGAC 2 cut(s) 1008, 1100
BslI CCNNNNNNNGG 7 cut(s) 326, 414, 440, 496, 689, 760, 850
BsmAI GTCTC 1 cut(s) 606
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 2 cut(s) 1008, 1100
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 6 cut(s) 38, 405, 712, 976, 1011, 1043
BsoBI CYCGRG 1 cut(s) 850
Bsp1286I GDGCHC 4 cut(s) 54, 67, 319, 533
Bsp1407I TGTACA 1 cut(s) 238
Bsp143I GATC 3 cut(s) 430, 466, 910
Bsp1720I GCTNAGC 3 cut(s) 307, 839, 861
Bsp19I CCATGG 1 cut(s) 688
BspACI CCGC 1 cut(s) 973
BspANI GGCC 6 cut(s) 38, 405, 712, 976, 1011, 1043
BspCNI CTCAG 3 cut(s) 275, 407, 853
BspDI ATCGAT 1 cut(s) 747
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 4 cut(s) 406, 530, 977, 1042
BspOI GCTAGC 1 cut(s) 105
BspPI GGATC 3 cut(s) 425, 474, 918
BspQI GCTCTTC 1 cut(s) 810
BspT107I GGYRCC 1 cut(s) 528
BsrGI TGTACA 1 cut(s) 238
BsrI ACTGG 2 cut(s) 425, 765
BssECI CCNNGG 2 cut(s) 688, 978
BssMI GATC 3 cut(s) 430, 466, 910
BssT1I CCWWGG 1 cut(s) 688
Bst2UI CCWGG 2 cut(s) 113, 980
Bst4CI ACNGT 4 cut(s) 175, 295, 659, 999
Bst6I CTCTTC 3 cut(s) 810, 933, 1096
BstAUI TGTACA 1 cut(s) 238
BstC8I GCNNGC 4 cut(s) 103, 279, 822, 1081
BstDEI CTNAG 7 cut(s) 21, 252, 262, 307, 394, 839, 861
BstDSI CCRYGG 1 cut(s) 688
BstENI CCTNNNNNAGG 1 cut(s) 494
BstF5I GGATG 3 cut(s) 505, 678, 684
BstKTI GATC 3 cut(s) 433, 469, 913
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 3 cut(s) 430, 466, 910
BstMWI GCNNNNNNNGC 1 cut(s) 1076
BstNI CCWGG 2 cut(s) 113, 980
BstSCI CCNGG 2 cut(s) 111, 978
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 2 cut(s) 54, 533
BstV1I GCAGC 3 cut(s) 261, 688, 904
Bsu15I ATCGAT 1 cut(s) 747
BsuI GTATCC 2 cut(s) 177, 226
BsuRI GGCC 6 cut(s) 38, 405, 712, 976, 1011, 1043
BsuTUI ATCGAT 1 cut(s) 747
BtgI CCRYGG 1 cut(s) 688
BtrI CACGTC 1 cut(s) 34
BtsCI GGATG 3 cut(s) 505, 678, 684
BtsI GCAGTG 1 cut(s) 1046
BtsIMutI CAGTG 6 cut(s) 105, 418, 664, 758, 957, 1046
Cac8I GCNNGC 4 cut(s) 103, 279, 822, 1081
CciI TCATGA 1 cut(s) 870
Cfr13I GGNCC 4 cut(s) 338, 404, 975, 1041
ClaI ATCGAT 1 cut(s) 747
CsiI ACCWGGT 1 cut(s) 111
Csp6I GTAC 7 cut(s) 16, 239, 425, 521, 577, 892, 1000
CviAII CATG 8 cut(s) 55, 581, 589, 669, 689, 871, 914, 1052
CviQI GTAC 7 cut(s) 16, 239, 425, 521, 577, 892, 1000
DdeI CTNAG 7 cut(s) 21, 252, 262, 307, 394, 839, 861
DpnI GATC 3 cut(s) 432, 468, 912
DpnII GATC 3 cut(s) 430, 466, 910
EaeI YGGCCR 2 cut(s) 36, 710
Eam1104I CTCTTC 3 cut(s) 810, 933, 1096
EarI CTCTTC 3 cut(s) 810, 933, 1096
Eco130I CCWWGG 1 cut(s) 688
Eco147I AGGCCT 1 cut(s) 1011
Eco47I GGWCC 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 249
Eco88I CYCGRG 1 cut(s) 850
EcoNI CCTNNNNNAGG 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 111, 978
EcoT14I CCWWGG 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 688
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 8 cut(s) 58, 584, 592, 672, 692, 874, 917, 1055
FaqI GGGAC 2 cut(s) 1008, 1100
FatI CATG 8 cut(s) 54, 580, 588, 668, 688, 870, 913, 1051
Fnu4HI GCNGC 4 cut(s) 275, 677, 918, 974
FokI GGATG 3 cut(s) 512, 671, 685
Fsp4HI GCNGC 4 cut(s) 275, 677, 918, 974
FspBI CTAG 4 cut(s) 102, 485, 524, 768
GluI GCNGC 4 cut(s) 275, 677, 918, 974
GsaI CCCAGC 3 cut(s) 758, 948, 1087
GsuI CTGGAG 2 cut(s) 333, 987
HaeIII GGCC 6 cut(s) 38, 405, 712, 976, 1011, 1043
Hin1II CATG 8 cut(s) 58, 584, 592, 672, 692, 874, 917, 1055
HinfI GANTC 4 cut(s) 134, 205, 260, 1088
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 2 cut(s) 52, 776
Hpy188I TCNGA 2 cut(s) 75, 193
Hpy188III TCNNGA 6 cut(s) 83, 355, 371, 614, 852, 871
Hpy8I GTNNAC 2 cut(s) 52, 776
HpyAV CCTTC 4 cut(s) 408, 618, 817, 1001
HpyCH4III ACNGT 4 cut(s) 175, 295, 659, 999
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 7 cut(s) 52, 152, 277, 390, 676, 881, 1051
HpyF10VI GCNNNNNNNGC 1 cut(s) 1076
HpyF3I CTNAG 7 cut(s) 21, 252, 262, 307, 394, 839, 861
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 8 cut(s) 58, 584, 592, 672, 692, 874, 917, 1055
Kzo9I GATC 3 cut(s) 430, 466, 910
LguI GCTCTTC 1 cut(s) 810
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 3 cut(s) 261, 688, 904
LweI GCATC 5 cut(s) 377, 663, 847, 952, 1057
MabI ACCWGGT 1 cut(s) 111
MaeI CTAG 4 cut(s) 102, 485, 524, 768
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 142, 955
MalI GATC 3 cut(s) 432, 468, 912
MboI GATC 3 cut(s) 430, 466, 910
MboII GAAGA 7 cut(s) 242, 245, 732, 827, 913, 950, 1083
MfeI CAATTG 1 cut(s) 147
MhlI GDGCHC 4 cut(s) 54, 67, 319, 533
MlsI TGGCCA 1 cut(s) 712
MluNI TGGCCA 1 cut(s) 712
MlyI GAGTC 3 cut(s) 214, 254, 1082
MmeI TCCRAC 1 cut(s) 9
Mox20I TGGCCA 1 cut(s) 712
MscI TGGCCA 1 cut(s) 712
MseI TTAA 1 cut(s) 333
Msp20I TGGCCA 1 cut(s) 712
MspA1I CMGCKG 1 cut(s) 1083
MspR9I CCNGG 2 cut(s) 113, 980
MunI CAATTG 1 cut(s) 147
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 2 cut(s) 113, 980
MwoI GCNNNNNNNGC 1 cut(s) 1076
NcoI CCATGG 1 cut(s) 688
NdeII GATC 3 cut(s) 430, 466, 910
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 8 cut(s) 58, 584, 592, 672, 692, 874, 917, 1055
NlaIV GGNNCC 4 cut(s) 406, 530, 977, 1042
NmuCI GTSAC 1 cut(s) 955
PagI TCATGA 1 cut(s) 870
PceI AGGCCT 1 cut(s) 1011
PciSI GCTCTTC 1 cut(s) 810
PctI GAATGC 1 cut(s) 308
PfeI GAWTC 1 cut(s) 134
PkrI GCNGC 4 cut(s) 276, 678, 919, 975
PleI GAGTC 3 cut(s) 213, 254, 1082
PpsI GAGTC 3 cut(s) 213, 254, 1082
Psp6I CCWGG 2 cut(s) 111, 978
PspFI CCCAGC 3 cut(s) 754, 944, 1083
PspGI CCWGG 2 cut(s) 111, 978
PspN4I GGNNCC 4 cut(s) 406, 530, 977, 1042
PspPI GGNCC 4 cut(s) 338, 404, 975, 1041
PvuII CAGCTG 1 cut(s) 1083
RsaI GTAC 7 cut(s) 17, 240, 426, 522, 578, 893, 1001
RsaNI GTAC 7 cut(s) 16, 239, 425, 521, 577, 892, 1000
SapI GCTCTTC 1 cut(s) 810
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 4 cut(s) 275, 677, 918, 974
Sau3AI GATC 3 cut(s) 430, 466, 910
Sau96I GGNCC 4 cut(s) 338, 404, 975, 1041
SchI GAGTC 3 cut(s) 214, 254, 1082
ScrFI CCNGG 2 cut(s) 113, 980
SduI GDGCHC 4 cut(s) 54, 67, 319, 533
SexAI ACCWGGT 1 cut(s) 111
SfaNI GCATC 5 cut(s) 377, 663, 847, 952, 1057
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 1 cut(s) 338
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SpeI ACTAGT 1 cut(s) 767
SseBI AGGCCT 1 cut(s) 1011
SsiI CCGC 1 cut(s) 973
SspMI CTAG 4 cut(s) 102, 485, 524, 768
StuI AGGCCT 1 cut(s) 1011
StyD4I CCNGG 2 cut(s) 111, 978
StyI CCWWGG 1 cut(s) 688
TaaI ACNGT 4 cut(s) 175, 295, 659, 999
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 747
TatI WGTACW 2 cut(s) 238, 999
TauI GCSGC 1 cut(s) 976
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 6 cut(s) 112, 425, 664, 765, 964, 1053
TseFI GTSAC 1 cut(s) 955
TseI GCWGC 3 cut(s) 274, 676, 917
Tsp45I GTSAC 1 cut(s) 955
TspDTI ATGAA 5 cut(s) 315, 560, 597, 859, 887
TspRI CASTG 6 cut(s) 112, 425, 664, 765, 964, 1053
VneI GTGCAC 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 338
XagI CCTNNNNNAGG 1 cut(s) 494
XapI RAATTY 4 cut(s) 194, 366, 715, 793
XspI CTAG 4 cut(s) 102, 485, 524, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.