Rorug04G0424700

PAP-specific phosphatase HAL2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002929
Physical Location & Seq
Forward (+)
58393401 .. 58396102
2702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0424700.1

Sequence Viewer

Length: 423 bp
ATGCCATTTTGCCTTCGTGTGACCTTGCCGGTTGAGGATCGAAGTTGCCTTGTTTCTCTGAACAATTTGAGGCCAGTTGCTTGCTTTTCCTATTCAAGTGATGTTAGGGAAAGGGATGACAGCCTTGTAATTAAGGATTTAAATCCAAGCCAGGGTAAGGCTGTGAATGGTAAAATAGCTGATCTAAGAGCTGCCATTAAATGCACCAAAGATTACAACCTCGAGTCTGAATACCCGTCCAAGACTGTTGAAATGCAAATAGTTCAGCTAGAAATGCTCAAGGAAAAATGGAGAGGTTTGGCGTCATCTCTGACAGCTTTACTCAAAGCTAGAGAAAAAGGCTTTAAGAGTATATTGGTTGAAGGGGATTCTAAACTCATCATTGATTGTGTAGATGGTGTCATTGGTGCCCTTGACAGTTGA

Protein Analysis

140

Amino Acids

15.49

Weight (kDa)

6.31

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 49 - 96 4.5e-07 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 407
AclWI GGATC 1 cut(s) 45
AcyI GRCGYC 1 cut(s) 302
AfiI CCNNNNNNNGG 2 cut(s) 152, 157
AgsI TTSAA 3 cut(s) 96, 251, 362
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 5 cut(s) 179, 191, 268, 317, 329
AluI AGCT 5 cut(s) 179, 191, 268, 317, 329
AlwI GGATC 1 cut(s) 45
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 221
BaeGI GKGCMC 1 cut(s) 412
BanI GGYRCC 1 cut(s) 407
BbvI GCAGC 1 cut(s) 178
BccI CCATC 1 cut(s) 389
BciT130I CCWGG 1 cut(s) 152
BfaI CTAG 2 cut(s) 269, 330
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
Bme1390I CCNGG 1 cut(s) 152
BmeT110I CYCGRG 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 409
BmrFI CCNGG 1 cut(s) 152
BpuEI CTTGAG 1 cut(s) 263
BsaBI GATNNNNATC 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 151
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 157
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 1 cut(s) 121
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 152, 157
BseNI ACTGG 1 cut(s) 74
BseSI GKGCMC 1 cut(s) 412
BseXI GCAGC 1 cut(s) 178
BshFI GGCC 1 cut(s) 73
BshNI GGYRCC 1 cut(s) 407
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 1 cut(s) 29
BslI CCNNNNNNNGG 2 cut(s) 152, 157
BsnI GGCC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 412
Bsp143I GATC 2 cut(s) 37, 181
BspANI GGCC 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 409
BspPI GGATC 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 407
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 2 cut(s) 37, 181
BssNI GRCGYC 1 cut(s) 302
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 2 cut(s) 247, 419
BstACI GRCGYC 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 1 cut(s) 185
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 2 cut(s) 40, 184
BstMBI GATC 2 cut(s) 37, 181
BstMWI GCNNNNNNNGC 1 cut(s) 274
BstNI CCWGG 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 412
BstV1I GCAGC 1 cut(s) 178
BsuRI GGCC 1 cut(s) 73
BtsCI GGATG 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 28
CseI GACGC 1 cut(s) 291
DdeI CTNAG 1 cut(s) 185
DpnI GATC 2 cut(s) 39, 183
DpnII GATC 2 cut(s) 37, 181
DraI TTTAAA 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 150
FaiI YATR 1 cut(s) 353
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 2 cut(s) 269, 330
GluI GCNGC 1 cut(s) 192
HaeIII GGCC 1 cut(s) 73
HapII CCGG 1 cut(s) 29
HgaI GACGC 1 cut(s) 291
Hin1I GRCGYC 1 cut(s) 302
HinfI GANTC 2 cut(s) 224, 368
HpaII CCGG 1 cut(s) 29
Hpy188I TCNGA 3 cut(s) 60, 229, 312
HpyAV CCTTC 2 cut(s) 23, 356
HpyCH4III ACNGT 2 cut(s) 247, 419
HpyCH4V TGCA 2 cut(s) 204, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 274
HpyF3I CTNAG 1 cut(s) 185
Hsp92I GRCGYC 1 cut(s) 302
Kzo9I GATC 2 cut(s) 37, 181
LpnPI CCDG 4 cut(s) 42, 87, 137, 164
Lsp1109I GCAGC 1 cut(s) 178
MaeI CTAG 2 cut(s) 269, 330
MaeIII GTNAC 1 cut(s) 19
MalI GATC 2 cut(s) 39, 183
MboI GATC 2 cut(s) 37, 181
MhlI GDGCHC 1 cut(s) 412
MluCI AATT 2 cut(s) 64, 129
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 4 cut(s) 28, 63, 230, 287
MseI TTAA 4 cut(s) 132, 140, 198, 345
MspI CCGG 1 cut(s) 29
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 274
NdeII GATC 2 cut(s) 37, 181
NlaIV GGNNCC 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 19
PaeR7I CTCGAG 1 cut(s) 221
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 409
PspXI VCTCGAGB 1 cut(s) 221
SaqAI TTAA 4 cut(s) 132, 140, 198, 345
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 2 cut(s) 37, 181
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 1 cut(s) 412
SetI ASST 8 cut(s) 26, 181, 193, 222, 270, 298, 319, 331
Sfr274I CTCGAG 1 cut(s) 221
SlaI CTCGAG 1 cut(s) 221
SmiI ATTTAAAT 1 cut(s) 141
SmlI CTYRAG 2 cut(s) 221, 278
SmoI CTYRAG 2 cut(s) 221, 278
Sse9I AATT 2 cut(s) 64, 129
SspMI CTAG 2 cut(s) 269, 330
StyD4I CCNGG 1 cut(s) 150
SwaI ATTTAAAT 1 cut(s) 141
TaaI ACNGT 2 cut(s) 247, 419
TaqI TCGA 2 cut(s) 40, 222
TasI AATT 2 cut(s) 64, 129
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 4 cut(s) 132, 140, 198, 345
Tru9I TTAA 4 cut(s) 132, 140, 198, 345
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 19
XhoI CTCGAG 1 cut(s) 221
XspI CTAG 2 cut(s) 269, 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.