FvH4_3g05760

Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004293
Physical Location & Seq
Forward (+)
3370464 .. 3372146
1683 bp
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UTR
Exon/CDS
Intron
FvH4_3g05760.t1

Sequence Viewer

Length: 537 bp
ATGGTTGCTCTCAAGTATCACCAGTACCAGGTCGTGGGTAGGGCCCTCCCCAAGGATGCTGAGGACACCCCGAAGATCTACCGTATGAAGCTTTGGCACACCAATGAGGTTCGCGCCAAATCCAAGTTCTGGTATTTCTTGAAGAAGCTGAAGAAGGTCAAGAAGAGCAATGGCCAAATGCTTGCCATCAATGAGATTTTTGAGAAGAAGCCAACTGTTGTTGAGAACTATGGTATTTGGTTGAGGTATCAAAGCAGGACAGGGTATCACAACATGTACAAGGAGTACCGTGACACCACTCTGAATGGGGCTGTGGATCAGATGTACAACGAGATGGCTTCTCGCCACAGGGTCAGGTTTCCTTGTATCCAAATTATCAAGATTGCCACTATCCCAGCTAAGCTTTGCAAGAGGGAATGTACTAAGCAATTCCACAATTCCAAGATCAAGTTTCCATTGATTACCAGGAAAGTCAGGCCACCTACCAGGAAGTTGAAGACCACGTACAAGGCATCGAGGCCCAACTTGTTTGTCTAA

Protein Analysis

179

Amino Acids

21.29

Weight (kDa)

10.24

Isoelectric Point (pI)

25.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18A PF01775 7 - 129 4.1e-24 Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 34, 129
AccII CGCG 1 cut(s) 114
AclWI GGATC 1 cut(s) 324
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 6 cut(s) 26, 278, 287, 326, 421, 506
AfiI CCNNNNNNNGG 4 cut(s) 28, 34, 52, 129
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 142, 496
AjnI CCWGG 3 cut(s) 27, 464, 485
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 4 cut(s) 91, 148, 398, 403
AluI AGCT 4 cut(s) 91, 148, 398, 403
AlwI GGATC 1 cut(s) 324
AoxI GGCC 4 cut(s) 42, 172, 476, 518
ApaI GGGCCC 1 cut(s) 46
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 3 cut(s) 42, 43, 519
AsuHPI GGTGA 1 cut(s) 11
BaeGI GKGCMC 1 cut(s) 46
BalI TGGCCA 1 cut(s) 174
BanII GRGCYC 1 cut(s) 46
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
BbsI GAAGAC 1 cut(s) 503
BbvCI CCTCAGC 1 cut(s) 60
BccI CCATC 2 cut(s) 194, 328
BciT130I CCWGG 3 cut(s) 29, 466, 487
BciVI GTATCC 1 cut(s) 377
BfuI GTATCC 1 cut(s) 377
BglII AGATCT 1 cut(s) 75
BlpI GCTNAGC 1 cut(s) 399
Bme1390I CCNGG 3 cut(s) 29, 466, 487
BmgT120I GGNCC 3 cut(s) 42, 43, 519
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 3 cut(s) 29, 466, 487
BmsI GCATC 2 cut(s) 46, 521
BpiI GAAGAC 1 cut(s) 503
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 399
BsaAI YACGTR 1 cut(s) 504
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 34, 52, 129
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 175
BseBI CCWGG 3 cut(s) 29, 466, 487
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 4 cut(s) 28, 34, 52, 129
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 22
BseSI GKGCMC 1 cut(s) 46
BseYI CCCAGC 1 cut(s) 394
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 4 cut(s) 44, 174, 478, 520
BslI CCNNNNNNNGG 4 cut(s) 28, 34, 52, 129
BsnI GGCC 4 cut(s) 44, 174, 478, 520
Bsp120I GGGCCC 1 cut(s) 42
Bsp1286I GDGCHC 1 cut(s) 46
Bsp1407I TGTACA 2 cut(s) 276, 324
Bsp143I GATC 3 cut(s) 75, 316, 444
Bsp1720I GCTNAGC 1 cut(s) 399
BspANI GGCC 4 cut(s) 44, 174, 478, 520
BspCNI CTCAG 1 cut(s) 52
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 44
BspPI GGATC 1 cut(s) 324
BspQI GCTCTTC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 175
BsrGI TGTACA 2 cut(s) 276, 324
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 3 cut(s) 75, 316, 444
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 3 cut(s) 29, 466, 487
Bst4CI ACNGT 3 cut(s) 83, 217, 290
Bst6I CTCTTC 1 cut(s) 158
BstAUI TGTACA 2 cut(s) 276, 324
BstBAI YACGTR 1 cut(s) 504
BstC8I GCNNGC 1 cut(s) 183
BstDEI CTNAG 3 cut(s) 60, 399, 423
BstENI CCTNNNNNAGG 1 cut(s) 50
BstF5I GGATG 1 cut(s) 61
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 3 cut(s) 78, 319, 447
BstMBI GATC 3 cut(s) 75, 316, 444
BstNI CCWGG 3 cut(s) 29, 466, 487
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 3 cut(s) 27, 464, 485
BstSLI GKGCMC 1 cut(s) 46
BstUI CGCG 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 4 cut(s) 44, 174, 478, 520
BtsCI GGATG 1 cut(s) 61
Cac8I GCNNGC 1 cut(s) 183
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 42, 43, 519
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 6 cut(s) 25, 277, 286, 325, 420, 505
CviAII CATG 1 cut(s) 274
CviQI GTAC 6 cut(s) 25, 277, 286, 325, 420, 505
DdeI CTNAG 3 cut(s) 60, 399, 423
DpnI GATC 3 cut(s) 77, 318, 446
DpnII GATC 3 cut(s) 75, 316, 444
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 50
EcoO109I RGGNCCY 2 cut(s) 42, 43
EcoRII CCWGG 3 cut(s) 27, 464, 485
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 1 cut(s) 277
FaiI YATR 3 cut(s) 86, 231, 275
FatI CATG 1 cut(s) 273
FokI GGATG 1 cut(s) 68
FriOI GRGCYC 1 cut(s) 46
GlaI GCGC 1 cut(s) 115
GsaI CCCAGC 1 cut(s) 398
HaeIII GGCC 4 cut(s) 44, 174, 478, 520
HhaI GCGC 1 cut(s) 116
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HindIII AAGCTT 2 cut(s) 89, 401
HphI GGTGA 1 cut(s) 11
Hpy188I TCNGA 2 cut(s) 303, 321
Hpy188III TCNNGA 3 cut(s) 139, 160, 379
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 3 cut(s) 83, 217, 290
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 1 cut(s) 408
HpyF3I CTNAG 3 cut(s) 60, 399, 423
HpySE526I ACGT 1 cut(s) 503
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 1 cut(s) 114
Kzo9I GATC 3 cut(s) 75, 316, 444
LguI GCTCTTC 1 cut(s) 158
LweI GCATC 2 cut(s) 46, 521
MabI ACCWGGT 1 cut(s) 27
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 1 cut(s) 290
MalI GATC 3 cut(s) 77, 318, 446
MboI GATC 3 cut(s) 75, 316, 444
MboII GAAGA 6 cut(s) 85, 154, 163, 175, 217, 508
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 1 cut(s) 46
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 3 cut(s) 372, 428, 436
MluNI TGGCCA 1 cut(s) 174
MnlI CCTC 6 cut(s) 55, 56, 100, 237, 405, 510
Mox20I TGGCCA 1 cut(s) 174
MscI TGGCCA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 102
Msp20I TGGCCA 1 cut(s) 174
MspR9I CCNGG 3 cut(s) 29, 466, 487
MvaI CCWGG 3 cut(s) 29, 466, 487
MvnI CGCG 1 cut(s) 114
NdeII GATC 3 cut(s) 75, 316, 444
NlaIII CATG 1 cut(s) 277
NlaIV GGNNCC 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 290
NspI RCATGY 1 cut(s) 277
PciI ACATGT 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 158
PflMI CCANNNNNTGG 2 cut(s) 34, 129
Ppu21I YACGTR 1 cut(s) 504
PscI ACATGT 1 cut(s) 273
Psp6I CCWGG 3 cut(s) 27, 464, 485
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 3 cut(s) 27, 464, 485
PspN4I GGNNCC 1 cut(s) 44
PspOMI GGGCCC 1 cut(s) 42
PspPI GGNCC 3 cut(s) 42, 43, 519
PsuI RGATCY 1 cut(s) 75
RsaI GTAC 6 cut(s) 26, 278, 287, 326, 421, 506
RsaNI GTAC 6 cut(s) 25, 277, 286, 325, 420, 505
RseI CAYNNNNRTG 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 158
Sau3AI GATC 3 cut(s) 75, 316, 444
Sau96I GGNCC 3 cut(s) 42, 43, 519
ScrFI CCNGG 3 cut(s) 29, 466, 487
SduI GDGCHC 1 cut(s) 46
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 2 cut(s) 46, 521
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 3 cut(s) 372, 428, 436
StyD4I CCNGG 3 cut(s) 27, 464, 485
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 3 cut(s) 83, 217, 290
TaiI ACGT 1 cut(s) 506
TaqI TCGA 1 cut(s) 515
TasI AATT 3 cut(s) 372, 428, 436
TatI WGTACW 3 cut(s) 276, 324, 419
TseFI GTSAC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 101
Van91I CCANNNNNTGG 2 cut(s) 34, 129
XagI CCTNNNNNAGG 1 cut(s) 50
XceI RCATGY 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.