RchiOBHm_Chr6g0245431

Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004293
Physical Location & Seq
Forward (+)
1213139 .. 1215484
2346 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21998

Sequence Viewer

Length: 522 bp
ATGGTCGCTCTCAAGTTTCACCAGTACCAGGTGGTGGGTAGGGCTCTCCCCAAGGATGCCGAGGACACCCCCAAGATCTACCGCATGAAGCTTTGGCACACCAATGATGTTCGCGCCAAGTCCAAGTTCTGGTATTTCCTGAAGAAGGTGAAGAAGAGCAATGGCCAAATTGTTGCCATCAATGAGATTTTGGAGAAGAAACCTACTGTTGTTGAGAACTACGGTATTTGGTTGAGGTATCAAAGCAGGACAGGGTATCACAACATGTACGAGGAGTACCGTGACACCACTCTGAATGGGGCTGTGGATCAGATGTGCACTGAGATGGCTTCTCGCCACAAGGTCAGGTTTCCTTGCATCCAAATCATCGAGACTGCCACGATCCCAGCTAAGCTTTGCAAGAGGGAAATTACCAAGCAATTCCACAATTCCAAGATCAATTTTCCATTGAACTCTGATGATGCATTTATGTCAGATTATGTTCGAATCCTCATTTTAGGTTCAGTCTGGCAAGGCTTCTGA

Protein Analysis

173

Amino Acids

20.37

Weight (kDa)

9.54

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18A PF01775 7 - 125 6.4e-20 Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 34, 129
AccII CGCG 1 cut(s) 114
AciI CCGC 1 cut(s) 82
AclWI GGATC 2 cut(s) 315, 376
AcoI YGGCCR 1 cut(s) 163
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 3 cut(s) 26, 269, 278
AfiI CCNNNNNNNGG 4 cut(s) 28, 34, 129, 145
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 1 cut(s) 451
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 3 cut(s) 91, 389, 394
AluI AGCT 3 cut(s) 91, 389, 394
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 1 cut(s) 365
Alw44I GTGCAC 1 cut(s) 316
AlwI GGATC 2 cut(s) 315, 376
AoxI GGCC 1 cut(s) 163
ApaLI GTGCAC 1 cut(s) 316
AspLEI GCGC 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 11, 160
AsuII TTCGAA 1 cut(s) 484
BaeGI GKGCMC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 165
BanII GRGCYC 1 cut(s) 46
Bbv12I GWGCWC 1 cut(s) 320
BccI CCATC 2 cut(s) 185, 319
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 365
BglII AGATCT 1 cut(s) 75
BlpI GCTNAGC 1 cut(s) 390
Bme1390I CCNGG 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 3 cut(s) 46, 366, 451
Bpu1102I GCTNAGC 1 cut(s) 390
Bpu14I TTCGAA 1 cut(s) 484
BsaJI CCNNGG 2 cut(s) 51, 60
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 34, 129, 145
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 166
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 51, 60
BseGI GGATG 2 cut(s) 61, 357
BseLI CCNNNNNNNGG 4 cut(s) 28, 34, 129, 145
BseMI GCAATG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 22
BseRI GAGGAG 1 cut(s) 287
BseSI GKGCMC 1 cut(s) 320
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 320
BslI CCNNNNNNNGG 4 cut(s) 28, 34, 129, 145
BsmAI GTCTC 1 cut(s) 365
BsnI GGCC 1 cut(s) 165
Bsp119I TTCGAA 1 cut(s) 484
Bsp1286I GDGCHC 2 cut(s) 46, 320
Bsp143I GATC 4 cut(s) 75, 307, 381, 435
Bsp1720I GCTNAGC 1 cut(s) 390
BspACI CCGC 1 cut(s) 82
BspANI GGCC 1 cut(s) 165
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 1 cut(s) 114
BspPI GGATC 2 cut(s) 315, 376
BspQI GCTCTTC 1 cut(s) 149
BspT104I TTCGAA 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 166
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 2 cut(s) 51, 60
BssMI GATC 4 cut(s) 75, 307, 381, 435
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 3 cut(s) 208, 224, 281
Bst6I CTCTTC 1 cut(s) 149
BstBI TTCGAA 1 cut(s) 484
BstDEI CTNAG 2 cut(s) 321, 390
BstENI CCTNNNNNAGG 1 cut(s) 143
BstF5I GGATG 2 cut(s) 61, 357
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 4 cut(s) 78, 310, 384, 438
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 4 cut(s) 75, 307, 381, 435
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 27
BstSLI GKGCMC 1 cut(s) 320
BstUI CGCG 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BsuRI GGCC 1 cut(s) 165
BtsCI GGATG 2 cut(s) 61, 357
BtsIMutI CAGTG 1 cut(s) 318
CfoI GCGC 1 cut(s) 116
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 3 cut(s) 25, 268, 277
CviAII CATG 2 cut(s) 85, 265
CviJI RGCY 8 cut(s) 44, 91, 165, 302, 329, 389, 394, 516
CviKI_1 RGCY 8 cut(s) 44, 91, 165, 302, 329, 389, 394, 516
CviQI GTAC 3 cut(s) 25, 268, 277
DdeI CTNAG 2 cut(s) 321, 390
DpnI GATC 4 cut(s) 77, 309, 383, 437
DpnII GATC 4 cut(s) 75, 307, 381, 435
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 161
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 2 cut(s) 88, 268
FaiI YATR 4 cut(s) 86, 266, 470, 480
FatI CATG 2 cut(s) 84, 264
FokI GGATG 2 cut(s) 68, 344
FriOI GRGCYC 1 cut(s) 46
GlaI GCGC 1 cut(s) 115
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 1 cut(s) 165
HhaI GCGC 1 cut(s) 116
Hin1II CATG 2 cut(s) 88, 268
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HindIII AAGCTT 2 cut(s) 89, 392
HinfI GANTC 1 cut(s) 486
HphI GGTGA 2 cut(s) 11, 160
Hpy166II GTNNAC 1 cut(s) 318
Hpy188I TCNGA 5 cut(s) 294, 312, 457, 475, 521
Hpy188III TCNNGA 2 cut(s) 139, 370
Hpy8I GTNNAC 1 cut(s) 318
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 3 cut(s) 208, 224, 281
HpyCH4V TGCA 4 cut(s) 318, 357, 399, 464
HpyF3I CTNAG 2 cut(s) 321, 390
Hsp92II CATG 2 cut(s) 88, 268
HspAI GCGC 1 cut(s) 114
Kzo9I GATC 4 cut(s) 75, 307, 381, 435
LguI GCTCTTC 1 cut(s) 149
LweI GCATC 3 cut(s) 46, 366, 451
MabI ACCWGGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 281
MalI GATC 4 cut(s) 77, 309, 383, 437
MboI GATC 4 cut(s) 75, 307, 381, 435
MboII GAAGA 4 cut(s) 154, 163, 166, 208
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 2 cut(s) 46, 320
MlsI TGGCCA 1 cut(s) 165
MluCI AATT 5 cut(s) 168, 408, 419, 427, 439
MluNI TGGCCA 1 cut(s) 165
MnlI CCTC 5 cut(s) 55, 228, 265, 396, 500
Mox20I TGGCCA 1 cut(s) 165
Mph1103I ATGCAT 1 cut(s) 466
MscI TGGCCA 1 cut(s) 165
MslI CAYNNNNRTG 2 cut(s) 102, 323
Msp20I TGGCCA 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 1 cut(s) 114
NdeII GATC 4 cut(s) 75, 307, 381, 435
NlaIII CATG 2 cut(s) 88, 268
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 1 cut(s) 268
NspV TTCGAA 1 cut(s) 484
PciI ACATGT 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 486
PflMI CCANNNNNTGG 2 cut(s) 34, 129
PscI ACATGT 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 1 cut(s) 27
PsuI RGATCY 1 cut(s) 75
RsaI GTAC 3 cut(s) 26, 269, 278
RsaNI GTAC 3 cut(s) 25, 268, 277
RseI CAYNNNNRTG 2 cut(s) 102, 323
SapI GCTCTTC 1 cut(s) 149
Sau3AI GATC 4 cut(s) 75, 307, 381, 435
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 2 cut(s) 46, 320
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 3 cut(s) 46, 366, 451
SfuI TTCGAA 1 cut(s) 484
SmiMI CAYNNNNRTG 2 cut(s) 102, 323
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 5 cut(s) 168, 408, 419, 427, 439
SsiI CCGC 1 cut(s) 82
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 3 cut(s) 208, 224, 281
TaqI TCGA 2 cut(s) 369, 484
TasI AATT 5 cut(s) 168, 408, 419, 427, 439
TfiI GAWTC 1 cut(s) 486
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 1 cut(s) 101
TspRI CASTG 1 cut(s) 325
Van91I CCANNNNNTGG 2 cut(s) 34, 129
VneI GTGCAC 1 cut(s) 316
XagI CCTNNNNNAGG 1 cut(s) 143
XceI RCATGY 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.