RchiOBHm_Chr1g0331531

Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004293
Physical Location & Seq
Reverse (-)
21383890 .. 21385868
1979 bp
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UTR
Exon/CDS
Intron
PRQ56055

Sequence Viewer

Length: 534 bp
ATGGTTGCTCTCAAGTTTCACCAGTACCAGGTGGTGGGTAGGGCTCTCCCCAAGGATGCCGAGGACACCCCCAAGATCTACCGCATGAAGCTTTGGCACACCAATGATGTTCGCGCCAAGTCCAAGTTCTGGTATTTCCTGAAGAAGGTGAAGAAGGTGAAGAAGAGCAATGGCCAAATTGTTGCCATCAATGAGATGTTGGAGAAGAAACCTACTGTTGTTGAGAACTACAGTATTTGGTTGAGGTATCAAAGTAGGACAGGGTATCACAACATGTACAAGGAGTACCGTGACACCACTCTGAATGGGGCTGTGGATCAGATGTGCACTGAGATGGCTTCTCGCCACAGGGTCAGGTTTCCTTGCATCCAAATCATCGAGACTGCCACGATCCCAGCTAAGCTTTGCAAGAGGGAAAGTACCAAGCAATTCCACAATTCCAAGATCAAGTTTCCATTGAACTCTGACGATGCATTTATGTCAGATTATGTTCGAATCCTCATTTTACTAATCGTGCATTGGACTGCTAACTGA

Protein Analysis

177

Amino Acids

20.96

Weight (kDa)

9.81

Isoelectric Point (pI)

26.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18A PF01775 7 - 128 3.1e-21 Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 34, 129
AccII CGCG 1 cut(s) 114
AciI CCGC 1 cut(s) 82
AclWI GGATC 2 cut(s) 324, 385
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 4 cut(s) 26, 278, 287, 421
AfiI CCNNNNNNNGG 4 cut(s) 28, 34, 129, 145
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 1 cut(s) 460
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 3 cut(s) 91, 398, 403
AluI AGCT 3 cut(s) 91, 398, 403
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 374
Alw44I GTGCAC 1 cut(s) 325
AlwI GGATC 2 cut(s) 324, 385
AoxI GGCC 1 cut(s) 172
ApaLI GTGCAC 1 cut(s) 325
AspLEI GCGC 1 cut(s) 116
AsuHPI GGTGA 3 cut(s) 11, 160, 169
AsuII TTCGAA 1 cut(s) 493
BaeGI GKGCMC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 174
BanII GRGCYC 1 cut(s) 46
Bbv12I GWGCWC 1 cut(s) 329
BccI CCATC 2 cut(s) 194, 328
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 374
BfmI CTRYAG 1 cut(s) 229
BglII AGATCT 1 cut(s) 75
BlpI GCTNAGC 1 cut(s) 399
Bme1390I CCNGG 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 3 cut(s) 46, 375, 460
Bpu1102I GCTNAGC 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 51, 60
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 34, 129, 145
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 51, 60
BseGI GGATG 2 cut(s) 61, 366
BseLI CCNNNNNNNGG 4 cut(s) 28, 34, 129, 145
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 1 cut(s) 22
BseSI GKGCMC 1 cut(s) 329
BseYI CCCAGC 1 cut(s) 394
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 329
BslI CCNNNNNNNGG 4 cut(s) 28, 34, 129, 145
BsmAI GTCTC 1 cut(s) 374
BsnI GGCC 1 cut(s) 174
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 2 cut(s) 46, 329
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 4 cut(s) 75, 316, 390, 444
Bsp1720I GCTNAGC 1 cut(s) 399
BspACI CCGC 1 cut(s) 82
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 1 cut(s) 114
BspPI GGATC 2 cut(s) 324, 385
BspQI GCTCTTC 1 cut(s) 158
BspT104I TTCGAA 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 175
BsrGI TGTACA 1 cut(s) 276
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 2 cut(s) 51, 60
BssMI GATC 4 cut(s) 75, 316, 390, 444
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 3 cut(s) 217, 233, 290
Bst6I CTCTTC 1 cut(s) 158
BstAUI TGTACA 1 cut(s) 276
BstBI TTCGAA 1 cut(s) 493
BstDEI CTNAG 2 cut(s) 330, 399
BstENI CCTNNNNNAGG 1 cut(s) 143
BstF5I GGATG 2 cut(s) 61, 366
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 4 cut(s) 78, 319, 393, 447
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 4 cut(s) 75, 316, 390, 444
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 229
BstSLI GKGCMC 1 cut(s) 329
BstUI CGCG 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BsuRI GGCC 1 cut(s) 174
BtsCI GGATG 2 cut(s) 61, 366
BtsIMutI CAGTG 1 cut(s) 327
CfoI GCGC 1 cut(s) 116
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 4 cut(s) 25, 277, 286, 420
CviAII CATG 2 cut(s) 85, 274
CviJI RGCY 7 cut(s) 44, 91, 174, 311, 338, 398, 403
CviKI_1 RGCY 7 cut(s) 44, 91, 174, 311, 338, 398, 403
CviQI GTAC 4 cut(s) 25, 277, 286, 420
DdeI CTNAG 2 cut(s) 330, 399
DpnI GATC 4 cut(s) 77, 318, 392, 446
DpnII GATC 4 cut(s) 75, 316, 390, 444
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 161
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 2 cut(s) 88, 277
FaiI YATR 4 cut(s) 86, 275, 479, 489
FatI CATG 2 cut(s) 84, 273
FokI GGATG 2 cut(s) 68, 353
FriOI GRGCYC 1 cut(s) 46
GlaI GCGC 1 cut(s) 115
GsaI CCCAGC 1 cut(s) 398
HaeIII GGCC 1 cut(s) 174
HhaI GCGC 1 cut(s) 116
Hin1II CATG 2 cut(s) 88, 277
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HindIII AAGCTT 2 cut(s) 89, 401
HinfI GANTC 1 cut(s) 495
HphI GGTGA 3 cut(s) 11, 160, 169
Hpy166II GTNNAC 1 cut(s) 327
Hpy188I TCNGA 4 cut(s) 303, 321, 466, 484
Hpy188III TCNNGA 2 cut(s) 139, 379
Hpy8I GTNNAC 1 cut(s) 327
HpyAV CCTTC 2 cut(s) 139, 148
HpyCH4III ACNGT 3 cut(s) 217, 233, 290
HpyCH4V TGCA 5 cut(s) 327, 366, 408, 473, 517
HpyF3I CTNAG 2 cut(s) 330, 399
Hsp92II CATG 2 cut(s) 88, 277
HspAI GCGC 1 cut(s) 114
Kzo9I GATC 4 cut(s) 75, 316, 390, 444
LguI GCTCTTC 1 cut(s) 158
LpnPI CCDG 9 cut(s) 14, 35, 41, 115, 152, 246, 334, 340, 408
LweI GCATC 3 cut(s) 46, 375, 460
MabI ACCWGGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 290
MalI GATC 4 cut(s) 77, 318, 392, 446
MboI GATC 4 cut(s) 75, 316, 390, 444
MboII GAAGA 5 cut(s) 154, 163, 172, 175, 217
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 2 cut(s) 46, 329
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 3 cut(s) 177, 428, 436
MluNI TGGCCA 1 cut(s) 174
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 4 cut(s) 55, 237, 405, 509
Mox20I TGGCCA 1 cut(s) 174
Mph1103I ATGCAT 1 cut(s) 475
MscI TGGCCA 1 cut(s) 174
MslI CAYNNNNRTG 2 cut(s) 102, 332
Msp20I TGGCCA 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 1 cut(s) 114
NdeII GATC 4 cut(s) 75, 316, 390, 444
NlaIII CATG 2 cut(s) 88, 277
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 475
NspI RCATGY 1 cut(s) 277
NspV TTCGAA 1 cut(s) 493
PciI ACATGT 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 495
PflMI CCANNNNNTGG 2 cut(s) 34, 129
PscI ACATGT 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 27
PsuI RGATCY 1 cut(s) 75
RsaI GTAC 4 cut(s) 26, 278, 287, 421
RsaNI GTAC 4 cut(s) 25, 277, 286, 420
RseI CAYNNNNRTG 2 cut(s) 102, 332
SapI GCTCTTC 1 cut(s) 158
Sau3AI GATC 4 cut(s) 75, 316, 390, 444
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 2 cut(s) 46, 329
SetI ASST 9 cut(s) 33, 93, 150, 159, 214, 248, 359, 400, 405
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 3 cut(s) 46, 375, 460
SfcI CTRYAG 1 cut(s) 229
SfuI TTCGAA 1 cut(s) 493
SmiMI CAYNNNNRTG 2 cut(s) 102, 332
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 3 cut(s) 177, 428, 436
SsiI CCGC 1 cut(s) 82
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 3 cut(s) 217, 233, 290
TaqI TCGA 2 cut(s) 378, 493
TasI AATT 3 cut(s) 177, 428, 436
TatI WGTACW 1 cut(s) 276
TfiI GAWTC 1 cut(s) 495
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 101
TspRI CASTG 1 cut(s) 334
Van91I CCANNNNNTGG 2 cut(s) 34, 129
VneI GTGCAC 1 cut(s) 325
XagI CCTNNNNNAGG 1 cut(s) 143
XceI RCATGY 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.