FvH4_3g19450

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003786
Physical Location & Seq
Forward (+)
12612546 .. 12613943
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19450.t1

Sequence Viewer

Length: 753 bp
ATGGCTCCGAAAACGGCAGCGAAATCCGGCACCGGAGCGCAGACGAACCCTCTTGAGATCCGGTATCGTGGCGTCAGGAAGAGGCCGTGGGGACGCTACGCCGCCGAGATCAGGGATCCGGGGAAGAAGACGAGGGTGTGGCTCGGTACGTTTGATACGGCTGAGGAGGCGGCGCGTGCGTACGATAAGGCGGCGAGGGAGTACCGTGGAGGTAAGGCCAAGACAAACTTTCCAACGCCGGCCGAGCTTCACGCTAACAACAACCGGAATAGCAATAACAACGGCAGACAGAATAATACCCATCATGCCCCGCCGGTTGCTCCTCCGGCGCGCAGCCCCAGCCAGACCAGCACTGTGGACTCCTCGTCCCCACCGCCGCCGGCCCCCCCGGCTCTGGACCTCACCCTCGCAGCTCCCCGTTTAGCGGTGGCCAACGCGGGATACTACACCGCCGCGAGTGCCTACCACGCGCTTCCCATGGGGCGTCCCATCTATTTCTTCGACGCGTTCGCGAGGGCCGACCAGAACGTGGTGACGATGACGAACACGAACAGTCTCCATCGCCCCACCGCCCGCGAGATGGGAAAGTTTGACAAAAGCTTCGCGGCCGCCGCTTCTCCCTCCGGCGGGTCCCACTCAGGCGTTAGCGACTCCGACTCCTCCACCGTCGTGGATTTCGCTCACTCCCCCGCCAAGAAAGGACCTTCGCTCGATCTCGATCTCAACCTCCCACCTCCACTGGATAATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009873 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010104 GO:0010105 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016604 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032870 GO:0033993 GO:0035556 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070297 GO:0070298 GO:0070887 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

251

Amino Acids

26.62

Weight (kDa)

9.81

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 21 - 70 2e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 29
AccB7I CCANNNNNTGG 1 cut(s) 529
AccII CGCG 9 cut(s) 175, 331, 437, 455, 470, 506, 512, 576, 605
AclWI GGATC 3 cut(s) 52, 110, 123
AcoI YGGCCR 3 cut(s) 240, 429, 606
AcyI GRCGYC 2 cut(s) 72, 484
AfaI GTAC 3 cut(s) 148, 182, 203
AfiI CCNNNNNNNGG 7 cut(s) 111, 394, 424, 529, 580, 626, 627
AflIII ACRYGT 1 cut(s) 504
AhdI GACNNNNNGTC 1 cut(s) 364
AleI CACNNNNGTG 1 cut(s) 668
AluBI AGCT 3 cut(s) 247, 413, 600
AluI AGCT 3 cut(s) 247, 413, 600
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 3 cut(s) 52, 110, 123
AoxI GGCC 7 cut(s) 83, 216, 240, 381, 429, 516, 606
ApeKI GCWGC 3 cut(s) 17, 333, 410
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
AspLEI GCGC 5 cut(s) 40, 175, 331, 333, 472
AspS9I GGNCC 5 cut(s) 382, 397, 516, 630, 701
AsuC2I CCSGG 2 cut(s) 120, 389
AsuHPI GGTGA 2 cut(s) 394, 544
AvaII GGWCC 3 cut(s) 397, 630, 701
BalI TGGCCA 1 cut(s) 431
BamHI GGATCC 1 cut(s) 115
BanI GGYRCC 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 134
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 29, 345, 422
BccI CCATC 4 cut(s) 309, 497, 567, 574
BceAI ACGGC 4 cut(s) 30, 70, 174, 298
BciVI GTATCC 1 cut(s) 434
BcnI CCSGG 2 cut(s) 120, 389
BcoDI GTCTC 1 cut(s) 560
BfuI GTATCC 1 cut(s) 434
BglI GCCNNNNNGGC 1 cut(s) 389
Bme1390I CCNGG 2 cut(s) 120, 389
Bme18I GGWCC 3 cut(s) 397, 630, 701
BmeRI GACNNNNNGTC 1 cut(s) 364
BmgT120I GGNCC 5 cut(s) 382, 397, 516, 630, 701
BmiI GGNNCC 6 cut(s) 6, 31, 117, 384, 631, 632
BmrFI CCNGG 2 cut(s) 120, 389
BpiI GAAGAC 1 cut(s) 134
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 74
BpuMI CCSGG 2 cut(s) 120, 389
BsaBI GATNNNNATC 1 cut(s) 717
BsaHI GRCGYC 2 cut(s) 72, 484
BsaJI CCNNGG 5 cut(s) 86, 119, 205, 387, 477
BsaWI WCCGGW 3 cut(s) 32, 60, 264
BsaXI ACNNNNNCTCC 2 cut(s) 641, 671
Bsc4I CCNNNNNNNGG 7 cut(s) 111, 394, 424, 529, 580, 626, 627
Bse118I RCCGGY 3 cut(s) 238, 313, 379
Bse1I ACTGG 1 cut(s) 744
Bse8I GATNNNNATC 1 cut(s) 717
BseDI CCNNGG 5 cut(s) 86, 119, 205, 387, 477
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 7 cut(s) 111, 394, 424, 529, 580, 626, 627
BseMII CTCAG 2 cut(s) 153, 651
BseNI ACTGG 1 cut(s) 744
BsePI GCGCGC 1 cut(s) 329
BseRI GAGGAG 4 cut(s) 179, 312, 352, 649
BseX3I CGGCCG 2 cut(s) 240, 606
BseXI GCAGC 3 cut(s) 29, 345, 422
BseYI CCCAGC 1 cut(s) 338
Bsh1236I CGCG 9 cut(s) 175, 331, 437, 455, 470, 506, 512, 576, 605
Bsh1285I CGRYCG 2 cut(s) 243, 609
BshFI GGCC 7 cut(s) 85, 218, 242, 383, 431, 518, 608
BshNI GGYRCC 1 cut(s) 29
BsiEI CGRYCG 2 cut(s) 243, 609
BsiWI CGTACG 1 cut(s) 180
BslFI GGGAC 4 cut(s) 105, 352, 471, 616
BslI CCNNNNNNNGG 7 cut(s) 111, 394, 424, 529, 580, 626, 627
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 4 cut(s) 105, 352, 471, 616
BsnI GGCC 7 cut(s) 85, 218, 242, 383, 431, 518, 608
Bsp143I GATC 5 cut(s) 57, 108, 115, 712, 718
Bsp19I CCATGG 1 cut(s) 477
Bsp68I TCGCGA 1 cut(s) 512
BspANI GGCC 7 cut(s) 85, 218, 242, 383, 431, 518, 608
BspCNI CTCAG 2 cut(s) 154, 650
BspFNI CGCG 9 cut(s) 175, 331, 437, 455, 470, 506, 512, 576, 605
BspLI GGNNCC 6 cut(s) 6, 31, 117, 384, 631, 632
BspPI GGATC 3 cut(s) 52, 110, 123
BspT107I GGYRCC 1 cut(s) 29
BsrFI RCCGGY 3 cut(s) 238, 313, 379
BsrI ACTGG 1 cut(s) 744
BssAI RCCGGY 3 cut(s) 238, 313, 379
BssECI CCNNGG 5 cut(s) 86, 119, 205, 387, 477
BssHII GCGCGC 1 cut(s) 329
BssMI GATC 5 cut(s) 57, 108, 115, 712, 718
BssNI GRCGYC 2 cut(s) 72, 484
BssT1I CCWWGG 1 cut(s) 477
Bst4CI ACNGT 4 cut(s) 206, 355, 554, 667
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 2 cut(s) 72, 484
BstC8I GCNNGC 5 cut(s) 177, 240, 331, 381, 574
BstDEI CTNAG 2 cut(s) 162, 637
BstDSI CCRYGG 3 cut(s) 86, 205, 477
BstFNI CGCG 9 cut(s) 175, 331, 437, 455, 470, 506, 512, 576, 605
BstHHI GCGC 5 cut(s) 40, 175, 331, 333, 472
BstKTI GATC 5 cut(s) 60, 111, 118, 715, 721
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 5 cut(s) 57, 108, 115, 712, 718
BstMCI CGRYCG 2 cut(s) 243, 609
BstSCI CCNGG 2 cut(s) 118, 387
BstUI CGCG 9 cut(s) 175, 331, 437, 455, 470, 506, 512, 576, 605
BstV1I GCAGC 3 cut(s) 29, 345, 422
BstV2I GAAGAC 1 cut(s) 134
BstX2I RGATCY 2 cut(s) 57, 115
BstXI CCANNNNNNTGG 2 cut(s) 355, 670
BstYI RGATCY 2 cut(s) 57, 115
BstZI CGGCCG 2 cut(s) 240, 606
BsuI GTATCC 1 cut(s) 434
BsuRI GGCC 7 cut(s) 85, 218, 242, 383, 431, 518, 608
BtgI CCRYGG 3 cut(s) 86, 205, 477
BtgZI GCGATG 1 cut(s) 545
BtsIMutI CAGTG 2 cut(s) 351, 737
BtuMI TCGCGA 1 cut(s) 512
Cac8I GCNNGC 5 cut(s) 177, 240, 331, 381, 574
CciNI GCGGCCGC 1 cut(s) 606
CfoI GCGC 5 cut(s) 40, 175, 331, 333, 472
Cfr10I RCCGGY 3 cut(s) 238, 313, 379
Cfr13I GGNCC 5 cut(s) 382, 397, 516, 630, 701
CseI GACGC 4 cut(s) 61, 102, 473, 512
Csp6I GTAC 3 cut(s) 147, 181, 202
CviAII CATG 2 cut(s) 305, 478
CviQI GTAC 3 cut(s) 147, 181, 202
DdeI CTNAG 2 cut(s) 162, 637
DpnI GATC 5 cut(s) 59, 110, 117, 714, 720
DpnII GATC 5 cut(s) 57, 108, 115, 712, 718
DriI GACNNNNNGTC 1 cut(s) 364
EaeI YGGCCR 3 cut(s) 240, 429, 606
EagI CGGCCG 2 cut(s) 240, 606
Eam1104I CTCTTC 1 cut(s) 74
Eam1105I GACNNNNNGTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 74
EclXI CGGCCG 2 cut(s) 240, 606
Eco130I CCWWGG 1 cut(s) 477
Eco47I GGWCC 3 cut(s) 397, 630, 701
Eco52I CGGCCG 2 cut(s) 240, 606
EcoO109I RGGNCCY 2 cut(s) 630, 701
EcoT14I CCWWGG 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 2 cut(s) 308, 481
FaiI YATR 2 cut(s) 306, 479
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FaqI GGGAC 4 cut(s) 105, 352, 471, 616
FatI CATG 2 cut(s) 304, 477
FauI CCCGC 5 cut(s) 318, 430, 581, 620, 697
GlaI GCGC 5 cut(s) 39, 174, 330, 332, 471
GsaI CCCAGC 1 cut(s) 342
HaeIII GGCC 7 cut(s) 85, 218, 242, 383, 431, 518, 608
HgaI GACGC 4 cut(s) 61, 102, 473, 512
HhaI GCGC 5 cut(s) 40, 175, 331, 333, 472
Hin1I GRCGYC 2 cut(s) 72, 484
Hin1II CATG 2 cut(s) 308, 481
Hin6I GCGC 5 cut(s) 38, 173, 329, 331, 470
HinP1I GCGC 5 cut(s) 38, 173, 329, 331, 470
HindIII AAGCTT 1 cut(s) 598
HinfI GANTC 3 cut(s) 359, 650, 656
HphI GGTGA 2 cut(s) 394, 544
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 2 cut(s) 9, 655
Hpy188III TCNNGA 5 cut(s) 53, 76, 395, 511, 716
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 2 cut(s) 506, 671
HpyAV CCTTC 1 cut(s) 714
HpyCH4III ACNGT 4 cut(s) 206, 355, 554, 667
HpyCH4IV ACGT 2 cut(s) 149, 528
HpyF3I CTNAG 2 cut(s) 162, 637
HpySE526I ACGT 2 cut(s) 149, 528
Hsp92I GRCGYC 2 cut(s) 72, 484
Hsp92II CATG 2 cut(s) 308, 481
HspAI GCGC 5 cut(s) 38, 173, 329, 331, 470
KflI GGGWCCC 1 cut(s) 630
KroI GCCGGC 2 cut(s) 238, 379
KroNI GCCGGC 2 cut(s) 240, 381
Kzo9I GATC 5 cut(s) 57, 108, 115, 712, 718
LmnI GCTCC 4 cut(s) 10, 35, 325, 418
Lsp1109I GCAGC 3 cut(s) 29, 345, 422
MaeII ACGT 2 cut(s) 149, 528
MaeIII GTNAC 1 cut(s) 532
MalI GATC 5 cut(s) 59, 110, 117, 714, 720
MboI GATC 5 cut(s) 57, 108, 115, 712, 718
MboII GAAGA 4 cut(s) 91, 136, 139, 490
MflI RGATCY 2 cut(s) 57, 115
MlsI TGGCCA 1 cut(s) 431
MluI ACGCGT 1 cut(s) 504
MluNI TGGCCA 1 cut(s) 431
MlyI GAGTC 3 cut(s) 353, 644, 650
MmeI TCCRAC 2 cut(s) 257, 678
Mox20I TGGCCA 1 cut(s) 431
MroNI GCCGGC 2 cut(s) 238, 379
MscI TGGCCA 1 cut(s) 431
MslI CAYNNNNRTG 1 cut(s) 668
Msp20I TGGCCA 1 cut(s) 431
MspR9I CCNGG 2 cut(s) 120, 389
MvnI CGCG 9 cut(s) 175, 331, 437, 455, 470, 506, 512, 576, 605
NaeI GCCGGC 2 cut(s) 240, 381
NciI CCSGG 2 cut(s) 120, 389
NcoI CCATGG 1 cut(s) 477
NdeII GATC 5 cut(s) 57, 108, 115, 712, 718
NgoMIV GCCGGC 2 cut(s) 238, 379
NlaIII CATG 2 cut(s) 308, 481
NlaIV GGNNCC 6 cut(s) 6, 31, 117, 384, 631, 632
NmeAIII GCCGAG 2 cut(s) 130, 268
NmuCI GTSAC 1 cut(s) 532
NotI GCGGCCGC 1 cut(s) 606
NruI TCGCGA 1 cut(s) 512
OliI CACNNNNGTG 1 cut(s) 668
PauI GCGCGC 1 cut(s) 329
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PdiI GCCGGC 2 cut(s) 240, 381
Pfl23II CGTACG 1 cut(s) 180
PflMI CCANNNNNTGG 1 cut(s) 529
PleI GAGTC 3 cut(s) 353, 644, 650
PpsI GAGTC 3 cut(s) 353, 644, 650
PpuMI RGGWCCY 2 cut(s) 630, 701
Psp5II RGGWCCY 2 cut(s) 630, 701
PspFI CCCAGC 1 cut(s) 338
PspLI CGTACG 1 cut(s) 180
PspN4I GGNNCC 6 cut(s) 6, 31, 117, 384, 631, 632
PspPI GGNCC 5 cut(s) 382, 397, 516, 630, 701
PspPPI RGGWCCY 2 cut(s) 630, 701
PsuI RGATCY 2 cut(s) 57, 115
PteI GCGCGC 1 cut(s) 329
RruI TCGCGA 1 cut(s) 512
RsaI GTAC 3 cut(s) 148, 182, 203
RsaNI GTAC 3 cut(s) 147, 181, 202
RseI CAYNNNNRTG 1 cut(s) 668
Sau3AI GATC 5 cut(s) 57, 108, 115, 712, 718
Sau96I GGNCC 5 cut(s) 382, 397, 516, 630, 701
SchI GAGTC 3 cut(s) 353, 644, 650
ScrFI CCNGG 2 cut(s) 120, 389
SinI GGWCC 3 cut(s) 397, 630, 701
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
StyD4I CCNGG 2 cut(s) 118, 387
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 4 cut(s) 206, 355, 554, 667
TaiI ACGT 2 cut(s) 152, 531
TaqI TCGA 3 cut(s) 501, 711, 717
TauI GCSGC 8 cut(s) 104, 173, 194, 379, 455, 608, 611, 614
TscAI CASTG 2 cut(s) 358, 744
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 3 cut(s) 17, 333, 410
Tsp45I GTSAC 1 cut(s) 532
TspRI CASTG 2 cut(s) 358, 744
Van91I CCANNNNNTGG 1 cut(s) 529
VpaK11BI GGWCC 3 cut(s) 397, 630, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.