MD10G1290400.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003786
Physical Location & Seq
Forward (+)
37888270 .. 37889088
819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1290400.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGCGCCGAGAGAGAAGACGGCCACGGCCGTTAGGGTGAACGGTAACGGAAACGTGAAGGAGGTGCATTTTAGAGGCGTGAGGAAGAGGCCGTGGGGAAGGTACGCCGCCGAGATCAGAGATCCCGGCAAGAAGAGCCGGGTCTGGCTCGGAACCTTCGACACGGCGGAGGAAGCCGCCCTGGCCTACGACAACGCCGCCAGAGATTTCCGCGGTGCCAAGGCCAAGACAAACTTCCCTCTCCTTTCGGAAAATGTTAGCCTGAGTATTACAAAAAATAATAATATTAATAATATAAATATTAATAATAATATAAATAATAATAATAAAATTAGTAGCGGAAGCAACAATCAAAGCCCGAGCCAGAGCAGCACGGTGGAGTCCTCCAGCCGTGAGCCGCCGGCTCTGATGGTCGATTCCTCGCCGTTTGATCTAAATCTCGCTCACGGTGTTAGCTCCGGCGGATTCGGCTCCGCGCCGATGAGGTTCCCCTTCCAGCACCACCAGGTTCCGGGAATCGGACCTTTTATCGGAGTCCCGGCTTCCTCCGCGGCAGCCGCGAATCACATGCTCTATTTCGACTCGGTGTTCAGAGCCGGCATGTTGAAGAACCACCAAATTCAACAACGGCTGAGATTCGACCAGGCCAACTTCCACCAGCATGATATCCAAGCCGCCGGTGGTGGTGGTGGTTTCGCCGGTGGTGCCCAGAGCGACTCCGACTCGTCCTCGGTCGTCGATCTGAACCAGAACGATCTGCCTCGCGGCGGCGGCGGAGGGTTCGATCTCAACTTCCCTCCTCCACCGGAACTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

273

Amino Acids

29.1

Weight (kDa)

9.25

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 23 - 72 2.3e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 4, 215, 704
AccII CGCG 5 cut(s) 213, 476, 551, 560, 765
AclWI GGATC 1 cut(s) 116
AcoI YGGCCR 2 cut(s) 21, 27
AcsI RAATTY 1 cut(s) 618
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 4 cut(s) 511, 518, 530, 767
AgsI TTSAA 2 cut(s) 607, 623
AjnI CCWGG 3 cut(s) 180, 504, 642
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 1 cut(s) 456
AluI AGCT 1 cut(s) 456
AlwI GGATC 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 6 cut(s) 21, 27, 89, 183, 222, 645
ApeKI GCWGC 2 cut(s) 369, 554
ApoI RAATTY 1 cut(s) 618
AseI ATTAAT 2 cut(s) 288, 303
AspLEI GCGC 2 cut(s) 7, 478
AspS9I GGNCC 1 cut(s) 521
AsuC2I CCSGG 4 cut(s) 126, 140, 513, 539
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 1 cut(s) 521
BaeGI GKGCMC 1 cut(s) 709
BanI GGYRCC 3 cut(s) 4, 215, 704
BbsI GAAGAC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 381, 566
BccI CCATC 1 cut(s) 403
BceAI ACGGC 8 cut(s) 14, 36, 42, 76, 180, 375, 409, 644
BcgI CGANNNNNNTGC 2 cut(s) 550, 584
BciT130I CCWGG 3 cut(s) 182, 506, 644
BcnI CCSGG 4 cut(s) 126, 140, 513, 539
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 182
Bme1390I CCNGG 7 cut(s) 126, 140, 182, 506, 513, 539, 644
Bme18I GGWCC 1 cut(s) 521
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 521
BmiI GGNNCC 7 cut(s) 6, 154, 217, 472, 488, 510, 706
BmrFI CCNGG 7 cut(s) 126, 140, 182, 506, 513, 539, 644
BpiI GAAGAC 1 cut(s) 23
BpmI CTGGAG 1 cut(s) 370
BpuMI CCSGG 4 cut(s) 126, 140, 513, 539
BsaBI GATNNNNATC 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 7 cut(s) 24, 92, 180, 211, 219, 549, 729
BsaWI WCCGGW 1 cut(s) 805
Bsc4I CCNNNNNNNGG 4 cut(s) 511, 518, 530, 767
Bse118I RCCGGY 4 cut(s) 400, 596, 677, 698
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 3 cut(s) 182, 506, 644
BseDI CCNNGG 7 cut(s) 24, 92, 180, 211, 219, 549, 729
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 4 cut(s) 511, 518, 530, 767
BseMII CTCAG 2 cut(s) 254, 623
BseRI GAGGAG 1 cut(s) 789
BseSI GKGCMC 1 cut(s) 709
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 381, 566
Bsh1236I CGCG 5 cut(s) 213, 476, 551, 560, 765
Bsh1285I CGRYCG 2 cut(s) 30, 735
BshFI GGCC 6 cut(s) 23, 29, 91, 185, 224, 647
BshNI GGYRCC 3 cut(s) 4, 215, 704
BsiEI CGRYCG 2 cut(s) 30, 735
BsiHKCI CYCGRG 1 cut(s) 358
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 4 cut(s) 511, 518, 530, 767
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 6 cut(s) 23, 29, 91, 185, 224, 647
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 1 cut(s) 709
Bsp143I GATC 6 cut(s) 114, 121, 430, 739, 754, 784
BspANI GGCC 6 cut(s) 23, 29, 91, 185, 224, 647
BspCNI CTCAG 2 cut(s) 255, 624
BspFNI CGCG 5 cut(s) 213, 476, 551, 560, 765
BspLI GGNNCC 7 cut(s) 6, 154, 217, 472, 488, 510, 706
BspPI GGATC 1 cut(s) 116
BspQI GCTCTTC 1 cut(s) 128
BspT107I GGYRCC 3 cut(s) 4, 215, 704
BsrFI RCCGGY 4 cut(s) 400, 596, 677, 698
BssAI RCCGGY 4 cut(s) 400, 596, 677, 698
BssECI CCNNGG 7 cut(s) 24, 92, 180, 211, 219, 549, 729
BssMI GATC 6 cut(s) 114, 121, 430, 739, 754, 784
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 3 cut(s) 182, 506, 644
Bst4CI ACNGT 3 cut(s) 44, 376, 449
Bst6I CTCTTC 2 cut(s) 80, 128
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 402, 598
BstDEI CTNAG 2 cut(s) 263, 632
BstDSI CCRYGG 4 cut(s) 24, 92, 211, 549
BstFNI CGCG 5 cut(s) 213, 476, 551, 560, 765
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 478
BstKTI GATC 6 cut(s) 117, 124, 433, 742, 757, 787
BstMBI GATC 6 cut(s) 114, 121, 430, 739, 754, 784
BstMCI CGRYCG 2 cut(s) 30, 735
BstMWI GCNNNNNNNGC 9 cut(s) 135, 173, 182, 369, 468, 548, 557, 704, 771
BstNI CCWGG 3 cut(s) 182, 506, 644
BstNSI RCATGY 2 cut(s) 571, 604
BstSCI CCNGG 7 cut(s) 124, 138, 180, 504, 511, 537, 642
BstSLI GKGCMC 1 cut(s) 709
BstUI CGCG 5 cut(s) 213, 476, 551, 560, 765
BstV1I GCAGC 2 cut(s) 381, 566
BstV2I GAAGAC 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 6 cut(s) 23, 29, 91, 185, 224, 647
BtgI CCRYGG 4 cut(s) 24, 92, 211, 549
Cac8I GCNNGC 2 cut(s) 402, 598
CfoI GCGC 2 cut(s) 7, 478
Cfr10I RCCGGY 4 cut(s) 400, 596, 677, 698
Cfr13I GGNCC 1 cut(s) 521
Cfr42I CCGCGG 2 cut(s) 214, 552
CsiI ACCWGGT 1 cut(s) 504
Csp6I GTAC 1 cut(s) 103
CviAII CATG 3 cut(s) 568, 601, 662
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 2 cut(s) 263, 632
DinI GGCGCC 1 cut(s) 6
DpnI GATC 6 cut(s) 116, 123, 432, 741, 756, 786
DpnII GATC 6 cut(s) 114, 121, 430, 739, 754, 784
EaeI YGGCCR 2 cut(s) 21, 27
EagI CGGCCG 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 80, 128
EarI CTCTTC 2 cut(s) 80, 128
EciI GGCGGA 3 cut(s) 182, 477, 789
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 219
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 521
Eco52I CGGCCG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 358
EcoRII CCWGG 3 cut(s) 180, 504, 642
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 219
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 3 cut(s) 571, 604, 665
FaiI YATR 5 cut(s) 296, 314, 569, 602, 663
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 521
FatI CATG 3 cut(s) 567, 600, 661
GlaI GCGC 2 cut(s) 6, 477
GsuI CTGGAG 1 cut(s) 370
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 23, 29, 91, 185, 224, 647
HhaI GCGC 2 cut(s) 7, 478
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 571, 604, 665
Hin6I GCGC 2 cut(s) 5, 476
HinP1I GCGC 2 cut(s) 5, 476
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 9 cut(s) 119, 152, 250, 408, 521, 533, 593, 721, 744
Hpy8I GTNNAC 1 cut(s) 40
Hpy99I CGWCG 1 cut(s) 740
HpyAV CCTTC 4 cut(s) 52, 93, 166, 502
HpyCH4III ACNGT 3 cut(s) 44, 376, 449
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 1 cut(s) 67
HpyF10VI GCNNNNNNNGC 9 cut(s) 135, 173, 182, 369, 468, 548, 557, 704, 771
HpyF3I CTNAG 2 cut(s) 263, 632
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 571, 604, 665
HspAI GCGC 2 cut(s) 5, 476
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 2 cut(s) 400, 596
KroNI GCCGGC 2 cut(s) 402, 598
KspI CCGCGG 2 cut(s) 214, 552
Kzo9I GATC 6 cut(s) 114, 121, 430, 739, 754, 784
LguI GCTCTTC 1 cut(s) 128
LmnI GCTCC 2 cut(s) 461, 476
Lsp1109I GCAGC 2 cut(s) 381, 566
MabI ACCWGGT 1 cut(s) 504
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 44
MalI GATC 6 cut(s) 116, 123, 432, 741, 756, 786
MboI GATC 6 cut(s) 114, 121, 430, 739, 754, 784
MboII GAAGA 4 cut(s) 28, 97, 145, 619
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 709
MluCI AATT 2 cut(s) 330, 618
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 5 cut(s) 389, 543, 575, 710, 716
MmeI TCCRAC 1 cut(s) 744
MroNI GCCGGC 2 cut(s) 400, 596
MseI TTAA 2 cut(s) 288, 303
MslI CAYNNNNRTG 1 cut(s) 660
MspA1I CMGCKG 2 cut(s) 213, 551
MspR9I CCNGG 7 cut(s) 126, 140, 182, 506, 513, 539, 644
MvaI CCWGG 3 cut(s) 182, 506, 644
MvnI CGCG 5 cut(s) 213, 476, 551, 560, 765
MwoI GCNNNNNNNGC 9 cut(s) 135, 173, 182, 369, 468, 548, 557, 704, 771
NaeI GCCGGC 2 cut(s) 402, 598
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 4 cut(s) 126, 140, 513, 539
NdeII GATC 6 cut(s) 114, 121, 430, 739, 754, 784
NgoMIV GCCGGC 2 cut(s) 400, 596
NlaIII CATG 3 cut(s) 571, 604, 665
NlaIV GGNNCC 7 cut(s) 6, 154, 217, 472, 488, 510, 706
NmeAIII GCCGAG 2 cut(s) 33, 136
NspI RCATGY 2 cut(s) 571, 604
PciSI GCTCTTC 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PdiI GCCGGC 2 cut(s) 402, 598
PfeI GAWTC 5 cut(s) 416, 465, 516, 562, 636
PfoI TCCNGGA 1 cut(s) 511
PleI GAGTC 5 cut(s) 388, 542, 575, 710, 716
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 5 cut(s) 388, 542, 575, 710, 716
PshBI ATTAAT 2 cut(s) 288, 303
Psp6I CCWGG 3 cut(s) 180, 504, 642
PspGI CCWGG 3 cut(s) 180, 504, 642
PspN4I GGNNCC 7 cut(s) 6, 154, 217, 472, 488, 510, 706
PspPI GGNCC 1 cut(s) 521
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 660
SacII CCGCGG 2 cut(s) 214, 552
SapI GCTCTTC 1 cut(s) 128
SaqAI TTAA 2 cut(s) 288, 303
Sau3AI GATC 6 cut(s) 114, 121, 430, 739, 754, 784
Sau96I GGNCC 1 cut(s) 521
SchI GAGTC 5 cut(s) 389, 543, 575, 710, 716
ScrFI CCNGG 7 cut(s) 126, 140, 182, 506, 513, 539, 644
SduI GDGCHC 1 cut(s) 709
SetI ASST 8 cut(s) 57, 66, 104, 158, 458, 488, 510, 526
SexAI ACCWGGT 1 cut(s) 504
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 2 cut(s) 214, 552
SgrAI CRCCGGYG 2 cut(s) 677, 698
SgrBI CCGCGG 2 cut(s) 214, 552
SinI GGWCC 1 cut(s) 521
SmiMI CAYNNNNRTG 1 cut(s) 660
Sse9I AATT 2 cut(s) 330, 618
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 2 cut(s) 286, 301
StyD4I CCNGG 7 cut(s) 124, 138, 180, 504, 511, 537, 642
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 3 cut(s) 44, 376, 449
TaiI ACGT 1 cut(s) 57
TaqI TCGA 6 cut(s) 159, 414, 579, 639, 738, 783
TaqII GACCGA 1 cut(s) 721
TasI AATT 2 cut(s) 330, 618
TfiI GAWTC 5 cut(s) 416, 465, 516, 562, 636
Tru1I TTAA 2 cut(s) 288, 303
Tru9I TTAA 2 cut(s) 288, 303
TseI GCWGC 2 cut(s) 369, 554
TspGWI ACGGA 1 cut(s) 63
VpaK11BI GGWCC 1 cut(s) 521
VspI ATTAAT 2 cut(s) 288, 303
XapI RAATTY 1 cut(s) 618
XceI RCATGY 2 cut(s) 571, 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.