RchiOBHm_Chr5g0032721

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003786
Physical Location & Seq
Forward (+)
26465310 .. 26466616
1307 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31188

Sequence Viewer

Length: 753 bp
ATGGCTCCGAAAACGGCAGCAAAATCCGGCTCCGGGACCCAGAACCCGAAGCCGAACTCGCTCGAGATCCGTTATCGGGGCGTCAGGAAGAGGCCTTGGGGTCGCTACGCTGCCGAGATCAGGGATCCTGGCAAGAAGACGAGGGTCTGGCTCGGCACGTTCGATACGGCTGAGGAGGCGGCGCGTGCGTACGACAAGGCGGCGCGGGAGTACCGTGGAGCTAAGGCTAAGACCAACTTCCCAACGCCGGCCGAGCTTCACGCGAACACAAACAACCGCGGCAACAATAACAGCGGCGGCAGACAGAACCATCATTCCCCGCCCGCGGCTCAGGCGCGCAGCCCCAGCCAGACCAGCACCGTGGACTCCTCGTCCCCGCCGGCGCAGGCCCCTCCGGCCCTGGACCTCACCCTCGCAGCGCCCCGTTTCGCGGGGGCCAACGCGGGCTACTTCACCGCCGCGAGTGCCTACCACGCGCTCCCCCTCGGGCGACCCATCTTTTTCTTCGACGCGTTCGCCAGGACCGACAACGTGGTGACGACGACGACGACGGGTCTCCATCGCCCCCTCGCCGCCCGCGAAATGGGCAAACTGGACAAAAGCGTCAAGGCAGCCGCCTCTCCGTCCGGCGGTTCCCTCTCCGGCCTCAGCGACTCGGACTCCTCCACCGTCGTGGATTTCGCTCACTCCCCCGCCAAGAAAGGACCGCTCGATCTCGACCTCAACCTCCCCCCTCCACCGGAGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009873 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010104 GO:0010105 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016604 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032870 GO:0033993 GO:0035556 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070297 GO:0070298 GO:0070887 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

250

Amino Acids

26.41

Weight (kDa)

10.03

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 24 - 73 1.9e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 602
AccBSI CCGCTC 1 cut(s) 709
AclWI GGATC 3 cut(s) 61, 119, 132
AcoI YGGCCR 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 2 cut(s) 191, 212
AfiI CCNNNNNNNGG 9 cut(s) 33, 76, 120, 247, 325, 430, 583, 629, 739
AflIII ACRYGT 1 cut(s) 510
AhdI GACNNNNNGTC 2 cut(s) 370, 552
AjnI CCWGG 3 cut(s) 127, 399, 518
AleI CACNNNNGTG 1 cut(s) 671
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
AluBI AGCT 2 cut(s) 221, 256
AluI AGCT 2 cut(s) 221, 256
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 3 cut(s) 61, 119, 132
Ama87I CYCGRG 2 cut(s) 62, 485
AoxI GGCC 6 cut(s) 92, 249, 387, 396, 435, 643
ApeKI GCWGC 5 cut(s) 17, 110, 339, 416, 611
AspLEI GCGC 7 cut(s) 184, 205, 337, 339, 385, 421, 478
AspS9I GGNCC 7 cut(s) 36, 388, 397, 403, 435, 522, 704
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 3 cut(s) 400, 445, 547
AvaI CYCGRG 2 cut(s) 62, 485
AvaII GGWCC 4 cut(s) 36, 403, 522, 704
BamHI GGATCC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 143
BbvCI CCTCAGC 2 cut(s) 171, 647
BbvI GCAGC 5 cut(s) 29, 97, 351, 428, 623
BccI CCATC 3 cut(s) 318, 503, 567
BceAI ACGGC 2 cut(s) 30, 183
BciT130I CCWGG 3 cut(s) 129, 401, 520
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 395
Bme1390I CCNGG 4 cut(s) 34, 129, 401, 520
Bme18I GGWCC 4 cut(s) 36, 403, 522, 704
BmeRI GACNNNNNGTC 2 cut(s) 370, 552
BmeT110I CYCGRG 2 cut(s) 62, 485
BmgT120I GGNCC 7 cut(s) 36, 388, 397, 403, 435, 522, 704
BmiI GGNNCC 8 cut(s) 6, 31, 37, 38, 126, 390, 436, 634
BmrFI CCNGG 4 cut(s) 34, 129, 401, 520
BoxI GACNNNNGTC 1 cut(s) 143
BpiI GAAGAC 1 cut(s) 143
Bpu10I CCTNAGC 4 cut(s) 171, 222, 330, 647
BpuMI CCSGG 1 cut(s) 34
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 7 cut(s) 95, 214, 277, 324, 360, 399, 484
BsaWI WCCGGW 1 cut(s) 739
BsaXI ACNNNNNCTCC 2 cut(s) 644, 674
Bsc4I CCNNNNNNNGG 9 cut(s) 33, 76, 120, 247, 325, 430, 583, 629, 739
Bse118I RCCGGY 2 cut(s) 247, 379
Bse1I ACTGG 1 cut(s) 597
BseBI CCWGG 3 cut(s) 129, 401, 520
BseDI CCNNGG 7 cut(s) 95, 214, 277, 324, 360, 399, 484
BseLI CCNNNNNNNGG 9 cut(s) 33, 76, 120, 247, 325, 430, 583, 629, 739
BseMII CTCAG 3 cut(s) 162, 344, 661
BseNI ACTGG 1 cut(s) 597
BsePI GCGCGC 1 cut(s) 335
BseRI GAGGAG 3 cut(s) 188, 358, 652
BseX3I CGGCCG 1 cut(s) 249
BseXI GCAGC 5 cut(s) 29, 97, 351, 428, 623
BseYI CCCAGC 1 cut(s) 344
Bsh1285I CGRYCG 1 cut(s) 252
BshFI GGCC 6 cut(s) 94, 251, 389, 398, 437, 645
BsiEI CGRYCG 1 cut(s) 252
BsiHKCI CYCGRG 2 cut(s) 62, 485
BsiSI CCGG 8 cut(s) 27, 33, 248, 380, 395, 627, 642, 740
BsiWI CGTACG 1 cut(s) 189
BslFI GGGAC 2 cut(s) 49, 358
BslI CCNNNNNNNGG 9 cut(s) 33, 76, 120, 247, 325, 430, 583, 629, 739
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 2 cut(s) 49, 358
BsnI GGCC 6 cut(s) 94, 251, 389, 398, 437, 645
Bso31I GGTCTC 1 cut(s) 560
BsoBI CYCGRG 2 cut(s) 62, 485
Bsp143I GATC 4 cut(s) 66, 117, 124, 712
BspANI GGCC 6 cut(s) 94, 251, 389, 398, 437, 645
BspCNI CTCAG 3 cut(s) 163, 343, 660
BspLI GGNNCC 8 cut(s) 6, 31, 37, 38, 126, 390, 436, 634
BspPI GGATC 3 cut(s) 61, 119, 132
BspTNI GGTCTC 1 cut(s) 560
BsrBI CCGCTC 1 cut(s) 709
BsrFI RCCGGY 2 cut(s) 247, 379
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 2 cut(s) 247, 379
BssECI CCNNGG 7 cut(s) 95, 214, 277, 324, 360, 399, 484
BssHII GCGCGC 1 cut(s) 335
BssMI GATC 4 cut(s) 66, 117, 124, 712
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 95
Bst2UI CCWGG 3 cut(s) 129, 401, 520
Bst4CI ACNGT 3 cut(s) 215, 361, 670
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 8 cut(s) 186, 249, 324, 337, 381, 387, 445, 577
BstDEI CTNAG 5 cut(s) 171, 222, 228, 330, 647
BstDSI CCRYGG 4 cut(s) 214, 277, 324, 360
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 7 cut(s) 184, 205, 337, 339, 385, 421, 478
BstKTI GATC 4 cut(s) 69, 120, 127, 715
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 4 cut(s) 66, 117, 124, 712
BstMCI CGRYCG 1 cut(s) 252
BstNI CCWGG 3 cut(s) 129, 401, 520
BstPAI GACNNNNGTC 1 cut(s) 143
BstSCI CCNGG 4 cut(s) 32, 127, 399, 518
BstV1I GCAGC 5 cut(s) 29, 97, 351, 428, 623
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 2 cut(s) 66, 124
BstXI CCANNNNNNTGG 2 cut(s) 361, 673
BstYI RGATCY 2 cut(s) 66, 124
BstZI CGGCCG 1 cut(s) 249
BsuRI GGCC 6 cut(s) 94, 251, 389, 398, 437, 645
BtgI CCRYGG 4 cut(s) 214, 277, 324, 360
BtgZI GCGATG 1 cut(s) 545
Cac8I GCNNGC 8 cut(s) 186, 249, 324, 337, 381, 387, 445, 577
CfoI GCGC 7 cut(s) 184, 205, 337, 339, 385, 421, 478
Cfr10I RCCGGY 2 cut(s) 247, 379
Cfr13I GGNCC 7 cut(s) 36, 388, 397, 403, 435, 522, 704
Cfr42I CCGCGG 2 cut(s) 280, 327
CseI GACGC 3 cut(s) 70, 518, 592
Csp6I GTAC 2 cut(s) 190, 211
CviQI GTAC 2 cut(s) 190, 211
DdeI CTNAG 5 cut(s) 171, 222, 228, 330, 647
DpnI GATC 4 cut(s) 68, 119, 126, 714
DpnII GATC 4 cut(s) 66, 117, 124, 712
DrdI GACNNNNNNGTC 1 cut(s) 602
DriI GACNNNNNGTC 2 cut(s) 370, 552
DseDI GACNNNNNNGTC 1 cut(s) 602
EaeI YGGCCR 1 cut(s) 249
EagI CGGCCG 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 83
Eam1105I GACNNNNNGTC 2 cut(s) 370, 552
EarI CTCTTC 1 cut(s) 83
EclXI CGGCCG 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 95
Eco147I AGGCCT 1 cut(s) 94
Eco31I GGTCTC 1 cut(s) 560
Eco47I GGWCC 4 cut(s) 36, 403, 522, 704
Eco52I CGGCCG 1 cut(s) 249
Eco88I CYCGRG 2 cut(s) 62, 485
EcoO109I RGGNCCY 2 cut(s) 36, 388
EcoRII CCWGG 3 cut(s) 127, 399, 518
EcoT14I CCWWGG 1 cut(s) 95
ErhI CCWWGG 1 cut(s) 95
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 2 cut(s) 49, 358
FauI CCCGC 8 cut(s) 198, 327, 331, 384, 424, 436, 584, 700
GlaI GCGC 7 cut(s) 183, 204, 336, 338, 384, 420, 477
GsaI CCCAGC 1 cut(s) 348
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 6 cut(s) 94, 251, 389, 398, 437, 645
HapII CCGG 8 cut(s) 27, 33, 248, 380, 395, 627, 642, 740
HgaI GACGC 3 cut(s) 70, 518, 592
HhaI GCGC 7 cut(s) 184, 205, 337, 339, 385, 421, 478
Hin1I GRCGYC 1 cut(s) 81
Hin6I GCGC 7 cut(s) 182, 203, 335, 337, 383, 419, 476
HinP1I GCGC 7 cut(s) 182, 203, 335, 337, 383, 419, 476
HinfI GANTC 3 cut(s) 365, 653, 659
HpaII CCGG 8 cut(s) 27, 33, 248, 380, 395, 627, 642, 740
HphI GGTGA 3 cut(s) 400, 445, 547
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 2 cut(s) 9, 658
Hpy188III TCNNGA 3 cut(s) 64, 85, 716
Hpy8I GTNNAC 1 cut(s) 364
Hpy99I CGWCG 6 cut(s) 512, 544, 547, 550, 553, 674
HpyCH4III ACNGT 3 cut(s) 215, 361, 670
HpyCH4IV ACGT 2 cut(s) 158, 531
HpyF3I CTNAG 5 cut(s) 171, 222, 228, 330, 647
HpySE526I ACGT 2 cut(s) 158, 531
Hsp92I GRCGYC 1 cut(s) 81
HspAI GCGC 7 cut(s) 182, 203, 335, 337, 383, 419, 476
KflI GGGWCCC 1 cut(s) 36
KroI GCCGGC 2 cut(s) 247, 379
KroNI GCCGGC 2 cut(s) 249, 381
KspI CCGCGG 2 cut(s) 280, 327
Kzo9I GATC 4 cut(s) 66, 117, 124, 712
LmnI GCTCC 4 cut(s) 10, 35, 218, 483
Lsp1109I GCAGC 5 cut(s) 29, 97, 351, 428, 623
MaeII ACGT 2 cut(s) 158, 531
MaeIII GTNAC 1 cut(s) 535
MalI GATC 4 cut(s) 68, 119, 126, 714
MbiI CCGCTC 1 cut(s) 709
MboI GATC 4 cut(s) 66, 117, 124, 712
MboII GAAGA 3 cut(s) 100, 148, 496
MflI RGATCY 2 cut(s) 66, 124
MluI ACGCGT 1 cut(s) 510
MlyI GAGTC 3 cut(s) 359, 647, 653
MreI CGCCGGCG 1 cut(s) 379
MroNI GCCGGC 2 cut(s) 247, 379
MslI CAYNNNNRTG 1 cut(s) 671
MspA1I CMGCKG 3 cut(s) 279, 294, 326
MspI CCGG 8 cut(s) 27, 33, 248, 380, 395, 627, 642, 740
MspR9I CCNGG 4 cut(s) 34, 129, 401, 520
MvaI CCWGG 3 cut(s) 129, 401, 520
NaeI GCCGGC 2 cut(s) 249, 381
NciI CCSGG 1 cut(s) 34
NdeII GATC 4 cut(s) 66, 117, 124, 712
NgoMIV GCCGGC 2 cut(s) 247, 379
NlaIV GGNNCC 8 cut(s) 6, 31, 37, 38, 126, 390, 436, 634
NmeAIII GCCGAG 3 cut(s) 132, 139, 277
NmuCI GTSAC 1 cut(s) 535
OliI CACNNNNGTG 1 cut(s) 671
PaeR7I CTCGAG 1 cut(s) 62
PauI GCGCGC 1 cut(s) 335
PceI AGGCCT 1 cut(s) 94
PcsI WCGNNNNNNNCGW 4 cut(s) 159, 522, 545, 576
PdiI GCCGGC 2 cut(s) 249, 381
Pfl23II CGTACG 1 cut(s) 189
PfoI TCCNGGA 1 cut(s) 32
PleI GAGTC 3 cut(s) 359, 647, 653
PpsI GAGTC 3 cut(s) 359, 647, 653
PpuMI RGGWCCY 1 cut(s) 36
PshAI GACNNNNGTC 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 36
Psp6I CCWGG 3 cut(s) 127, 399, 518
PspFI CCCAGC 1 cut(s) 344
PspGI CCWGG 3 cut(s) 127, 399, 518
PspLI CGTACG 1 cut(s) 189
PspN4I GGNNCC 8 cut(s) 6, 31, 37, 38, 126, 390, 436, 634
PspPI GGNCC 7 cut(s) 36, 388, 397, 403, 435, 522, 704
PspPPI RGGWCCY 1 cut(s) 36
PsuI RGATCY 2 cut(s) 66, 124
PteI GCGCGC 1 cut(s) 335
RsaI GTAC 2 cut(s) 191, 212
RsaNI GTAC 2 cut(s) 190, 211
RseI CAYNNNNRTG 1 cut(s) 671
SacII CCGCGG 2 cut(s) 280, 327
Sau3AI GATC 4 cut(s) 66, 117, 124, 712
Sau96I GGNCC 7 cut(s) 36, 388, 397, 403, 435, 522, 704
SchI GAGTC 3 cut(s) 359, 647, 653
ScrFI CCNGG 4 cut(s) 34, 129, 401, 520
SetI ASST 7 cut(s) 161, 223, 258, 408, 534, 723, 729
SfiI GGCCNNNNNGGCC 1 cut(s) 395
Sfr274I CTCGAG 1 cut(s) 62
Sfr303I CCGCGG 2 cut(s) 280, 327
SgrAI CRCCGGYG 1 cut(s) 379
SgrBI CCGCGG 2 cut(s) 280, 327
SinI GGWCC 4 cut(s) 36, 403, 522, 704
SlaI CTCGAG 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SseBI AGGCCT 1 cut(s) 94
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 4 cut(s) 32, 127, 399, 518
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 3 cut(s) 215, 361, 670
TaiI ACGT 2 cut(s) 161, 534
TaqI TCGA 5 cut(s) 63, 162, 507, 711, 717
TaqII GACCGA 1 cut(s) 539
TauI GCSGC 9 cut(s) 182, 203, 282, 297, 300, 329, 461, 575, 617
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 5 cut(s) 17, 110, 339, 416, 611
Tsp45I GTSAC 1 cut(s) 535
TspGWI ACGGA 2 cut(s) 59, 612
VpaK11BI GGWCC 4 cut(s) 36, 403, 522, 704
XhoI CTCGAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.