Rh5BG066400

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003786
Physical Location & Seq
Reverse (-)
5473758 .. 5475003
1246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG066400.1

Sequence Viewer

Length: 792 bp
ATGGCACCGAGAACCGAGAAAACCGCCACCACGGCTAAACCTAACGGCAACGTGAAGGAGGTGCATTTTAGGGGAGTGAGGAAGAGGCCGTGGGGGAGGTACGCGGCGGAGATTCGGGACCCGGGGAAGAAGAGCCGGGTCTGGCTCGGGACCTTCGACACAGCGGAGGAGGCGGCCAGAGCATACGATACGGCGGCGCGGGAGTTCCGCGGCGCCAAGGCCAAGACGAACTTCCCGCTGCCGTCGGAGAATGTGAATCTCAGCCTCTTCAAGAAAAATGTTGGGAGTAATAATATAAATAACAATAACAATAACAACGGAGGCGGAAATAGCAACAGCCACAGCCCTAGCCAGAGCAGCACGGTGGAGTCGTCCAGCCGGGAGCCGGCTATGATGGTTGAGTCCTCCTCGCCGTTGGATCTGAACCTCGCTCACGGCGTCAGTAGCGGCCACGCCGGATTCGGAGCTCCGATGAGGTTTCCCTTCCAGCACCACCAGATTCCGGCGATCGGAGCCGTTATCGGCGTTCCGACTTCGGCCGCGAATCACGTGCTCTACTTCGACTCGGTGTTCCGGGCCGGGATGGTGAAGAATCACCAGCAGTACCAGCAACAGCTGAGATTCGACCGGAATCTCATCCACCGTGATTTTCTCTCCGGCGGTGGCTTCACCGGCGGGTCGCAGAGTGACTCGGATTCGTCCACCGTCGTTGATCTGAACCACCACGATCTACCTCGCGGAGGAGGATTCGACCTCGACCTCAACCTGCCGCCTCCGGCGGAACTCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

263

Amino Acids

28.48

Weight (kDa)

9.39

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 22 - 71 7.4e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 774
AccB1I GGYRCC 2 cut(s) 4, 212
AccII CGCG 5 cut(s) 104, 199, 210, 542, 738
AclWI GGATC 1 cut(s) 426
AcoI YGGCCR 3 cut(s) 174, 448, 537
AcvI CACGTG 1 cut(s) 550
AcyI GRCGYC 2 cut(s) 213, 438
AfaI GTAC 2 cut(s) 101, 605
AfiI CCNNNNNNNGG 4 cut(s) 385, 502, 509, 740
AgsI TTSAA 1 cut(s) 271
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
AluBI AGCT 2 cut(s) 467, 616
AluI AGCT 2 cut(s) 467, 616
Alw21I GWGCWC 2 cut(s) 469, 555
AlwI GGATC 1 cut(s) 426
Ama87I CYCGRG 2 cut(s) 121, 146
AoxI GGCC 6 cut(s) 86, 174, 219, 448, 537, 576
ApeKI GCWGC 2 cut(s) 238, 357
AspLEI GCGC 2 cut(s) 199, 215
AspS9I GGNCC 3 cut(s) 118, 150, 576
AsuC2I CCSGG 6 cut(s) 122, 123, 137, 380, 575, 580
AsuHPI GGTGA 3 cut(s) 587, 598, 661
AvaI CYCGRG 2 cut(s) 121, 146
AvaII GGWCC 2 cut(s) 118, 150
BanI GGYRCC 2 cut(s) 4, 212
BanII GRGCYC 1 cut(s) 469
BbrPI CACGTG 1 cut(s) 550
Bbv12I GWGCWC 2 cut(s) 469, 555
BbvI GCAGC 2 cut(s) 225, 369
BccI CCATC 2 cut(s) 388, 577
BceAI ACGGC 8 cut(s) 48, 61, 73, 207, 226, 397, 451, 500
BcgI CGANNNNNNTGC 2 cut(s) 532, 566
BcnI CCSGG 6 cut(s) 122, 123, 137, 380, 575, 580
BfaI CTAG 1 cut(s) 348
BfoI RGCGCY 1 cut(s) 216
BfuAI ACCTGC 1 cut(s) 774
BglI GCCNNNNNGGC 1 cut(s) 32
BisI GCNGC 9 cut(s) 105, 174, 195, 211, 239, 358, 448, 540, 770
BlsI GCNGC 9 cut(s) 106, 175, 196, 212, 240, 359, 449, 541, 771
Bme1390I CCNGG 6 cut(s) 122, 123, 137, 380, 575, 580
Bme18I GGWCC 2 cut(s) 118, 150
BmeT110I CYCGRG 2 cut(s) 121, 146
BmgT120I GGNCC 3 cut(s) 118, 150, 576
BmiI GGNNCC 7 cut(s) 6, 119, 120, 151, 214, 384, 514
BmrFI CCNGG 6 cut(s) 122, 123, 137, 380, 575, 580
BplI GAGNNNNNCTC 2 cut(s) 392, 424
BpuMI CCSGG 6 cut(s) 122, 123, 137, 380, 575, 580
BsaAI YACGTR 1 cut(s) 550
BsaHI GRCGYC 2 cut(s) 213, 438
BsaJI CCNNGG 6 cut(s) 30, 89, 121, 122, 208, 216
BsaWI WCCGGW 1 cut(s) 627
Bsc4I CCNNNNNNNGG 4 cut(s) 385, 502, 509, 740
Bse118I RCCGGY 2 cut(s) 385, 671
BseDI CCNNGG 6 cut(s) 30, 89, 121, 122, 208, 216
BseGI GGATG 2 cut(s) 588, 636
BseLI CCNNNNNNNGG 4 cut(s) 385, 502, 509, 740
BseMII CTCAG 2 cut(s) 274, 608
BseRI GAGGAG 3 cut(s) 182, 397, 756
BseX3I CGGCCG 1 cut(s) 537
BseXI GCAGC 2 cut(s) 225, 369
Bsh1236I CGCG 5 cut(s) 104, 199, 210, 542, 738
Bsh1285I CGRYCG 3 cut(s) 510, 540, 628
BshFI GGCC 6 cut(s) 88, 176, 221, 450, 539, 578
BshNI GGYRCC 2 cut(s) 4, 212
BsiEI CGRYCG 3 cut(s) 510, 540, 628
BsiHKAI GWGCWC 2 cut(s) 469, 555
BsiHKCI CYCGRG 2 cut(s) 121, 146
BslFI GGGAC 2 cut(s) 131, 163
BslI CCNNNNNNNGG 4 cut(s) 385, 502, 509, 740
BsmFI GGGAC 2 cut(s) 131, 163
BsnI GGCC 6 cut(s) 88, 176, 221, 450, 539, 578
BsoBI CYCGRG 2 cut(s) 121, 146
Bsp1286I GDGCHC 2 cut(s) 469, 555
Bsp143I GATC 4 cut(s) 418, 507, 712, 727
BspANI GGCC 6 cut(s) 88, 176, 221, 450, 539, 578
BspCNI CTCAG 2 cut(s) 273, 609
BspFNI CGCG 5 cut(s) 104, 199, 210, 542, 738
BspLI GGNNCC 7 cut(s) 6, 119, 120, 151, 214, 384, 514
BspMI ACCTGC 1 cut(s) 774
BspPI GGATC 1 cut(s) 426
BspQI GCTCTTC 1 cut(s) 125
BspT107I GGYRCC 2 cut(s) 4, 212
BsrFI RCCGGY 2 cut(s) 385, 671
BssAI RCCGGY 2 cut(s) 385, 671
BssECI CCNNGG 6 cut(s) 30, 89, 121, 122, 208, 216
BssMI GATC 4 cut(s) 418, 507, 712, 727
BssNI GRCGYC 2 cut(s) 213, 438
BssT1I CCWWGG 1 cut(s) 216
Bst4CI ACNGT 3 cut(s) 364, 644, 706
Bst6I CTCTTC 3 cut(s) 77, 125, 272
BstACI GRCGYC 2 cut(s) 213, 438
BstBAI YACGTR 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 2 cut(s) 260, 617
BstDSI CCRYGG 3 cut(s) 30, 89, 208
BstENI CCTNNNNNAGG 1 cut(s) 738
BstF5I GGATG 2 cut(s) 588, 636
BstFNI CGCG 5 cut(s) 104, 199, 210, 542, 738
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 2 cut(s) 199, 215
BstKTI GATC 4 cut(s) 421, 510, 715, 730
BstMBI GATC 4 cut(s) 418, 507, 712, 727
BstMCI CGRYCG 3 cut(s) 510, 540, 628
BstSCI CCNGG 6 cut(s) 120, 121, 135, 378, 573, 578
BstUI CGCG 5 cut(s) 104, 199, 210, 542, 738
BstV1I GCAGC 2 cut(s) 225, 369
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BstZI CGGCCG 1 cut(s) 537
BsuRI GGCC 6 cut(s) 88, 176, 221, 450, 539, 578
BtgI CCRYGG 3 cut(s) 30, 89, 208
BtsCI GGATG 2 cut(s) 588, 636
BveI ACCTGC 1 cut(s) 774
Cac8I GCNNGC 1 cut(s) 387
CfoI GCGC 2 cut(s) 199, 215
Cfr10I RCCGGY 2 cut(s) 385, 671
Cfr13I GGNCC 3 cut(s) 118, 150, 576
Cfr42I CCGCGG 1 cut(s) 211
Cfr9I CCCGGG 1 cut(s) 121
CseI GACGC 1 cut(s) 427
Csp6I GTAC 2 cut(s) 100, 604
CspCI CAANNNNNGTGG 2 cut(s) 691, 726
CviQI GTAC 2 cut(s) 100, 604
DdeI CTNAG 2 cut(s) 260, 617
DinI GGCGCC 1 cut(s) 214
DpnI GATC 4 cut(s) 420, 509, 714, 729
DpnII GATC 4 cut(s) 418, 507, 712, 727
EaeI YGGCCR 3 cut(s) 174, 448, 537
EagI CGGCCG 1 cut(s) 537
Eam1104I CTCTTC 3 cut(s) 77, 125, 272
EarI CTCTTC 3 cut(s) 77, 125, 272
EciI GGCGGA 2 cut(s) 122, 339
Ecl136II GAGCTC 1 cut(s) 467
EclXI CGGCCG 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 469
Eco47I GGWCC 2 cut(s) 118, 150
Eco52I CGGCCG 1 cut(s) 537
Eco53kI GAGCTC 1 cut(s) 467
Eco72I CACGTG 1 cut(s) 550
Eco88I CYCGRG 2 cut(s) 121, 146
EcoICRI GAGCTC 1 cut(s) 467
EcoNI CCTNNNNNAGG 1 cut(s) 738
EcoO109I RGGNCCY 2 cut(s) 118, 150
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 469
EgeI GGCGCC 1 cut(s) 214
EheI GGCGCC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 216
FaiI YATR 3 cut(s) 184, 296, 392
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 2 cut(s) 131, 163
FauI CCCGC 3 cut(s) 192, 243, 668
Fnu4HI GCNGC 9 cut(s) 105, 174, 195, 211, 239, 358, 448, 540, 770
FokI GGATG 2 cut(s) 595, 623
FriOI GRGCYC 1 cut(s) 469
Fsp4HI GCNGC 9 cut(s) 105, 174, 195, 211, 239, 358, 448, 540, 770
FspBI CTAG 1 cut(s) 348
GlaI GCGC 2 cut(s) 198, 214
GluI GCNGC 9 cut(s) 105, 174, 195, 211, 239, 358, 448, 540, 770
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 6 cut(s) 88, 176, 221, 450, 539, 578
HgaI GACGC 1 cut(s) 427
HhaI GCGC 2 cut(s) 199, 215
Hin1I GRCGYC 2 cut(s) 213, 438
Hin6I GCGC 2 cut(s) 197, 213
HinP1I GCGC 2 cut(s) 197, 213
HphI GGTGA 3 cut(s) 587, 598, 661
Hpy166II GTNNAC 1 cut(s) 702
Hpy188I TCNGA 8 cut(s) 247, 423, 464, 471, 512, 531, 694, 717
Hpy188III TCNNGA 3 cut(s) 116, 148, 271
Hpy8I GTNNAC 1 cut(s) 702
Hpy99I CGWCG 2 cut(s) 247, 710
HpyAV CCTTC 3 cut(s) 49, 163, 493
HpyCH4III ACNGT 3 cut(s) 364, 644, 706
HpyCH4IV ACGT 2 cut(s) 51, 549
HpyCH4V TGCA 1 cut(s) 64
HpyF3I CTNAG 2 cut(s) 260, 617
HpySE526I ACGT 2 cut(s) 51, 549
Hsp92I GRCGYC 2 cut(s) 213, 438
HspAI GCGC 2 cut(s) 197, 213
KasI GGCGCC 1 cut(s) 212
KflI GGGWCCC 1 cut(s) 118
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
KspI CCGCGG 1 cut(s) 211
Kzo9I GATC 4 cut(s) 418, 507, 712, 727
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 4 cut(s) 382, 464, 472, 512
Lsp1109I GCAGC 2 cut(s) 225, 369
MaeI CTAG 1 cut(s) 348
MaeII ACGT 2 cut(s) 51, 549
MaeIII GTNAC 1 cut(s) 686
MalI GATC 4 cut(s) 420, 509, 714, 729
MboI GATC 4 cut(s) 418, 507, 712, 727
MboII GAAGA 5 cut(s) 94, 139, 142, 259, 601
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 2 cut(s) 469, 555
Mly113I GGCGCC 1 cut(s) 213
MlyI GAGTC 4 cut(s) 377, 410, 557, 683
MmeI TCCRAC 3 cut(s) 225, 396, 554
MroNI GCCGGC 1 cut(s) 385
MspA1I CMGCKG 4 cut(s) 164, 210, 238, 616
MspR9I CCNGG 6 cut(s) 122, 123, 137, 380, 575, 580
MvnI CGCG 5 cut(s) 104, 199, 210, 542, 738
NaeI GCCGGC 1 cut(s) 387
NarI GGCGCC 1 cut(s) 213
NciI CCSGG 6 cut(s) 122, 123, 137, 380, 575, 580
NdeII GATC 4 cut(s) 418, 507, 712, 727
NgoMIV GCCGGC 1 cut(s) 385
NlaIV GGNNCC 7 cut(s) 6, 119, 120, 151, 214, 384, 514
NmuCI GTSAC 1 cut(s) 686
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 3 cut(s) 153, 368, 435
PdiI GCCGGC 1 cut(s) 387
PkrI GCNGC 9 cut(s) 106, 175, 196, 212, 240, 359, 449, 541, 771
Ple19I CGATCG 1 cut(s) 510
PleI GAGTC 4 cut(s) 376, 409, 557, 683
PluTI GGCGCC 1 cut(s) 216
PmaCI CACGTG 1 cut(s) 550
PmlI CACGTG 1 cut(s) 550
PpsI GAGTC 4 cut(s) 376, 409, 557, 683
Ppu21I YACGTR 1 cut(s) 550
PpuMI RGGWCCY 2 cut(s) 118, 150
Psp124BI GAGCTC 1 cut(s) 469
Psp5II RGGWCCY 2 cut(s) 118, 150
PspCI CACGTG 1 cut(s) 550
PspN4I GGNNCC 7 cut(s) 6, 119, 120, 151, 214, 384, 514
PspPI GGNCC 3 cut(s) 118, 150, 576
PspPPI RGGWCCY 2 cut(s) 118, 150
PsuI RGATCY 1 cut(s) 418
PvuI CGATCG 1 cut(s) 510
PvuII CAGCTG 1 cut(s) 616
RsaI GTAC 2 cut(s) 101, 605
RsaNI GTAC 2 cut(s) 100, 604
SacI GAGCTC 1 cut(s) 469
SacII CCGCGG 1 cut(s) 211
SapI GCTCTTC 1 cut(s) 125
SatI GCNGC 9 cut(s) 105, 174, 195, 211, 239, 358, 448, 540, 770
Sau3AI GATC 4 cut(s) 418, 507, 712, 727
Sau96I GGNCC 3 cut(s) 118, 150, 576
SchI GAGTC 4 cut(s) 377, 410, 557, 683
ScrFI CCNGG 6 cut(s) 122, 123, 137, 380, 575, 580
SduI GDGCHC 2 cut(s) 469, 555
SfoI GGCGCC 1 cut(s) 214
Sfr303I CCGCGG 1 cut(s) 211
SgrAI CRCCGGYG 1 cut(s) 671
SgrBI CCGCGG 1 cut(s) 211
SinI GGWCC 2 cut(s) 118, 150
SmaI CCCGGG 1 cut(s) 123
SspDI GGCGCC 1 cut(s) 212
SspMI CTAG 1 cut(s) 348
SstI GAGCTC 1 cut(s) 469
StyD4I CCNGG 6 cut(s) 120, 121, 135, 378, 573, 578
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 3 cut(s) 364, 644, 706
TaiI ACGT 2 cut(s) 54, 552
TaqI TCGA 5 cut(s) 156, 561, 624, 750, 756
TauI GCSGC 7 cut(s) 107, 176, 197, 213, 450, 542, 772
TseFI GTSAC 1 cut(s) 686
TseI GCWGC 2 cut(s) 238, 357
Tsp45I GTSAC 1 cut(s) 686
TspGWI ACGGA 1 cut(s) 333
TspMI CCCGGG 1 cut(s) 121
VpaK11BI GGWCC 2 cut(s) 118, 150
XagI CCTNNNNNAGG 1 cut(s) 738
XmaI CCCGGG 1 cut(s) 121
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.