MD03G1231800.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003786
Physical Location & Seq
Reverse (-)
31718140 .. 31718832
693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1231800.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGCTCCGACGACGGCGAAGTCCCTACCCAAATCCGGCTCTGAGGACCAGAATCCGACATCCAACGAGATCCGGTACCGGGGTGTCCGGAAGAGGCCTTGGGGACGCTACGCCGCCGAGATCAGAGATCCCGGGAAGAAGACCCGCGTCTGGCTTGGGACCTTCGACACCGCCGAGGAGGCAGCGCGTGCGTACGACAAGGCCGCGCGTGAGTTTCGCGGAGGCAAGGCGAAGACTAACTTCCCCACCCCCTCTGAGCTCCAGCTCGACGCCGTCAACATCGTCAACATGAACAACGCCGCCAAATCAGCGGCGGCGACGAACGTCAGCAACAGTCCCAGCAGCCAGAGCAGCACCGTGGAGTCCTCCTCGCCGCCGCCTCCGCCGCCACTCGACCTCACTCTCAAAAACCCCCGTTTCTCCACCGGCGGCGGCTACTTCACCGCTGCGAGCGGCTTCCGCCCGCTCCCCGTGCCCCGTCCCGTGTTCTTCTTCGACGCCTTCGCACGGGCCGACGGCGCCGCCGCCCTCCAGCTCGCCCGCGAAACTTGCAGGTTCGACCGCCCCGCTCCCATATCCGGCTCGGCCCACAGCGACTCCTCCACGTCCTCGGTCGTCGACTTCGAGCGTAGCTCCCGCAACGTACGGCTCGATCTCGACCTGAACCTCCCAGCCCCCTCGGAAGTCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009873 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010104 GO:0010105 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016604 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032870 GO:0033993 GO:0035556 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070297 GO:0070298 GO:0070887 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

231

Amino Acids

24.72

Weight (kDa)

9.62

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 25 - 74 2.7e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 543
Acc65I GGTACC 1 cut(s) 75
AccB1I GGYRCC 2 cut(s) 75, 518
AccBSI CCGCTC 3 cut(s) 453, 466, 569
AccI GTMKAC 1 cut(s) 618
AccII CGCG 6 cut(s) 147, 187, 206, 208, 219, 543
AccIII TCCGGA 1 cut(s) 87
AclWI GGATC 2 cut(s) 64, 122
AcyI GRCGYC 3 cut(s) 270, 498, 519
AfaI GTAC 3 cut(s) 77, 194, 645
AfiI CCNNNNNNNGG 5 cut(s) 35, 79, 150, 507, 578
AjiI CACGTC 1 cut(s) 606
AluBI AGCT 4 cut(s) 259, 265, 535, 633
AluI AGCT 4 cut(s) 259, 265, 535, 633
Alw21I GWGCWC 1 cut(s) 261
AlwI GGATC 2 cut(s) 64, 122
Ama87I CYCGRG 1 cut(s) 131
Aor13HI TCCGGA 1 cut(s) 87
AoxI GGCC 4 cut(s) 95, 201, 510, 585
ApeKI GCWGC 4 cut(s) 182, 342, 351, 446
Asp718I GGTACC 1 cut(s) 75
AspLEI GCGC 3 cut(s) 187, 208, 521
AspS9I GGNCC 4 cut(s) 46, 159, 510, 586
AsuC2I CCSGG 3 cut(s) 80, 132, 133
AsuHPI GGTGA 1 cut(s) 433
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 2 cut(s) 46, 159
BaeGI GKGCMC 1 cut(s) 477
BaeI ACNNNNGTAYC 2 cut(s) 67, 100
BanI GGYRCC 2 cut(s) 75, 518
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 2 cut(s) 146, 239
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 194, 354, 363, 433
BceAI ACGGC 4 cut(s) 30, 257, 532, 662
BcnI CCSGG 3 cut(s) 80, 132, 133
BfoI RGCGCY 1 cut(s) 522
BfuAI ACCTGC 1 cut(s) 543
BglI GCCNNNNNGGC 1 cut(s) 179
Bme1390I CCNGG 3 cut(s) 80, 132, 133
Bme18I GGWCC 2 cut(s) 46, 159
BmeT110I CYCGRG 1 cut(s) 131
BmgBI CACGTC 1 cut(s) 606
BmgT120I GGNCC 4 cut(s) 46, 159, 510, 586
BmiI GGNNCC 4 cut(s) 6, 77, 160, 520
BmrFI CCNGG 3 cut(s) 80, 132, 133
BoxI GACNNNNGTC 2 cut(s) 146, 323
BpiI GAAGAC 2 cut(s) 146, 239
BplI GAGNNNNNCTC 4 cut(s) 353, 385, 617, 649
BpmI CTGGAG 2 cut(s) 245, 515
BpuMI CCSGG 3 cut(s) 80, 132, 133
BsaHI GRCGYC 3 cut(s) 270, 498, 519
BsaJI CCNNGG 7 cut(s) 79, 98, 131, 174, 357, 609, 678
BsaWI WCCGGW 2 cut(s) 72, 87
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 5 cut(s) 35, 79, 150, 507, 578
Bse118I RCCGGY 1 cut(s) 425
BseAI TCCGGA 1 cut(s) 87
BseDI CCNNGG 7 cut(s) 79, 98, 131, 174, 357, 609, 678
BseGI GGATG 1 cut(s) 59
BseLI CCNNNNNNNGG 5 cut(s) 35, 79, 150, 507, 578
BseMII CTCAG 2 cut(s) 33, 246
BseRI GAGGAG 3 cut(s) 191, 358, 589
BseSI GKGCMC 1 cut(s) 477
BseXI GCAGC 4 cut(s) 194, 354, 363, 433
BseYI CCCAGC 2 cut(s) 338, 670
Bsh1236I CGCG 6 cut(s) 147, 187, 206, 208, 219, 543
Bsh1285I CGRYCG 2 cut(s) 562, 615
BshFI GGCC 4 cut(s) 97, 203, 512, 587
BshNI GGYRCC 2 cut(s) 75, 518
BsiEI CGRYCG 2 cut(s) 562, 615
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 7 cut(s) 36, 73, 79, 88, 132, 426, 579
BsiWI CGTACG 2 cut(s) 192, 643
BslFI GGGAC 5 cut(s) 7, 117, 172, 321, 465
BslI CCNNNNNNNGG 5 cut(s) 35, 79, 150, 507, 578
BsmFI GGGAC 5 cut(s) 7, 117, 172, 321, 465
BsnI GGCC 4 cut(s) 97, 203, 512, 587
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 261, 477
Bsp13I TCCGGA 1 cut(s) 87
Bsp143I GATC 4 cut(s) 69, 120, 127, 652
BspANI GGCC 4 cut(s) 97, 203, 512, 587
BspCNI CTCAG 2 cut(s) 34, 247
BspEI TCCGGA 1 cut(s) 87
BspFNI CGCG 6 cut(s) 147, 187, 206, 208, 219, 543
BspLI GGNNCC 4 cut(s) 6, 77, 160, 520
BspMI ACCTGC 1 cut(s) 543
BspPI GGATC 2 cut(s) 64, 122
BspT107I GGYRCC 2 cut(s) 75, 518
BsrBI CCGCTC 3 cut(s) 453, 466, 569
BsrFI RCCGGY 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 7 cut(s) 79, 98, 131, 174, 357, 609, 678
BssMI GATC 4 cut(s) 69, 120, 127, 652
BssNI GRCGYC 3 cut(s) 270, 498, 519
BssT1I CCWWGG 1 cut(s) 98
Bst4CI ACNGT 2 cut(s) 335, 358
Bst6I CTCTTC 1 cut(s) 86
BstACI GRCGYC 3 cut(s) 270, 498, 519
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 5 cut(s) 189, 451, 464, 537, 541
BstDEI CTNAG 2 cut(s) 42, 255
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 59
BstFNI CGCG 6 cut(s) 147, 187, 206, 208, 219, 543
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 3 cut(s) 187, 208, 521
BstKTI GATC 4 cut(s) 72, 123, 130, 655
BstMBI GATC 4 cut(s) 69, 120, 127, 652
BstMCI CGRYCG 2 cut(s) 562, 615
BstPAI GACNNNNGTC 2 cut(s) 146, 323
BstSCI CCNGG 3 cut(s) 78, 130, 131
BstSLI GKGCMC 1 cut(s) 477
BstUI CGCG 6 cut(s) 147, 187, 206, 208, 219, 543
BstV1I GCAGC 4 cut(s) 194, 354, 363, 433
BstV2I GAAGAC 2 cut(s) 146, 239
BstX2I RGATCY 2 cut(s) 69, 127
BstYI RGATCY 2 cut(s) 69, 127
BsuRI GGCC 4 cut(s) 97, 203, 512, 587
BtgI CCRYGG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 606
BtsCI GGATG 1 cut(s) 59
BveI ACCTGC 1 cut(s) 543
Cac8I GCNNGC 5 cut(s) 189, 451, 464, 537, 541
CfoI GCGC 3 cut(s) 187, 208, 521
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 4 cut(s) 46, 159, 510, 586
Cfr9I CCCGGG 1 cut(s) 131
CseI GACGC 4 cut(s) 114, 136, 278, 506
Csp6I GTAC 3 cut(s) 76, 193, 644
CviAII CATG 1 cut(s) 289
CviQI GTAC 3 cut(s) 76, 193, 644
DdeI CTNAG 2 cut(s) 42, 255
DinI GGCGCC 1 cut(s) 520
DpnI GATC 4 cut(s) 71, 122, 129, 654
DpnII GATC 4 cut(s) 69, 120, 127, 652
DrdI GACNNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
EciI GGCGGA 2 cut(s) 372, 449
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 98
Eco147I AGGCCT 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 261
Eco47I GGWCC 2 cut(s) 46, 159
Eco53kI GAGCTC 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 261
EgeI GGCGCC 1 cut(s) 520
EheI GGCGCC 1 cut(s) 520
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 1 cut(s) 292
FaiI YATR 2 cut(s) 290, 575
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FaqI GGGAC 5 cut(s) 7, 117, 172, 321, 465
FatI CATG 1 cut(s) 288
FauI CCCGC 5 cut(s) 152, 471, 548, 574, 644
FblI GTMKAC 1 cut(s) 618
FokI GGATG 1 cut(s) 46
FriOI GRGCYC 1 cut(s) 261
GlaI GCGC 3 cut(s) 186, 207, 520
GsaI CCCAGC 2 cut(s) 342, 674
GsuI CTGGAG 2 cut(s) 245, 515
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 4 cut(s) 97, 203, 512, 587
HapII CCGG 7 cut(s) 36, 73, 79, 88, 132, 426, 579
HgaI GACGC 4 cut(s) 114, 136, 278, 506
HhaI GCGC 3 cut(s) 187, 208, 521
Hin1I GRCGYC 3 cut(s) 270, 498, 519
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 3 cut(s) 185, 206, 519
HinP1I GCGC 3 cut(s) 185, 206, 519
HincII GTYRAC 3 cut(s) 277, 286, 619
HindII GTYRAC 3 cut(s) 277, 286, 619
HinfI GANTC 3 cut(s) 52, 362, 596
HpaII CCGG 7 cut(s) 36, 73, 79, 88, 132, 426, 579
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 3 cut(s) 277, 286, 619
Hpy188I TCNGA 6 cut(s) 9, 43, 57, 125, 256, 682
Hpy188III TCNNGA 2 cut(s) 88, 656
Hpy8I GTNNAC 3 cut(s) 277, 286, 619
Hpy99I CGWCG 7 cut(s) 13, 16, 272, 322, 500, 518, 620
HpyAV CCTTC 2 cut(s) 172, 511
HpyCH4III ACNGT 2 cut(s) 335, 358
HpyCH4IV ACGT 3 cut(s) 324, 605, 642
HpyCH4V TGCA 1 cut(s) 552
HpyF3I CTNAG 2 cut(s) 42, 255
HpySE526I ACGT 3 cut(s) 324, 605, 642
Hsp92I GRCGYC 3 cut(s) 270, 498, 519
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 3 cut(s) 185, 206, 519
KasI GGCGCC 1 cut(s) 518
Kpn2I TCCGGA 1 cut(s) 87
KpnI GGTACC 1 cut(s) 79
Kzo9I GATC 4 cut(s) 69, 120, 127, 652
LmnI GCTCC 5 cut(s) 10, 264, 471, 574, 638
Lsp1109I GCAGC 4 cut(s) 194, 354, 363, 433
MaeII ACGT 3 cut(s) 324, 605, 642
MalI GATC 4 cut(s) 71, 122, 129, 654
MbiI CCGCTC 3 cut(s) 453, 466, 569
MboI GATC 4 cut(s) 69, 120, 127, 652
MboII GAAGA 6 cut(s) 103, 148, 151, 244, 481, 484
MflI RGATCY 2 cut(s) 69, 127
MhlI GDGCHC 2 cut(s) 261, 477
Mly113I GGCGCC 1 cut(s) 519
MlyI GAGTC 2 cut(s) 371, 590
MmeI TCCRAC 3 cut(s) 32, 80, 87
MroI TCCGGA 1 cut(s) 87
MspA1I CMGCKG 2 cut(s) 311, 446
MspI CCGG 7 cut(s) 36, 73, 79, 88, 132, 426, 579
MspR9I CCNGG 3 cut(s) 80, 132, 133
MvnI CGCG 6 cut(s) 147, 187, 206, 208, 219, 543
NarI GGCGCC 1 cut(s) 519
NciI CCSGG 3 cut(s) 80, 132, 133
NdeII GATC 4 cut(s) 69, 120, 127, 652
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 4 cut(s) 6, 77, 160, 520
NmeAIII GCCGAG 3 cut(s) 142, 199, 563
PceI AGGCCT 1 cut(s) 97
PcsI WCGNNNNNNNCGW 4 cut(s) 171, 510, 621, 648
PfeI GAWTC 1 cut(s) 52
Pfl23II CGTACG 2 cut(s) 192, 643
PflFI GACNNNGTC 1 cut(s) 272
PleI GAGTC 2 cut(s) 370, 590
PluTI GGCGCC 1 cut(s) 522
PpsI GAGTC 2 cut(s) 370, 590
PpuMI RGGWCCY 1 cut(s) 159
PshAI GACNNNNGTC 2 cut(s) 146, 323
Psp124BI GAGCTC 1 cut(s) 261
Psp5II RGGWCCY 1 cut(s) 159
PspFI CCCAGC 2 cut(s) 338, 670
PspLI CGTACG 2 cut(s) 192, 643
PspN4I GGNNCC 4 cut(s) 6, 77, 160, 520
PspPI GGNCC 4 cut(s) 46, 159, 510, 586
PspPPI RGGWCCY 1 cut(s) 159
PsuI RGATCY 2 cut(s) 69, 127
PsyI GACNNNGTC 1 cut(s) 272
RsaI GTAC 3 cut(s) 77, 194, 645
RsaNI GTAC 3 cut(s) 76, 193, 644
SacI GAGCTC 1 cut(s) 261
SalI GTCGAC 1 cut(s) 617
Sau3AI GATC 4 cut(s) 69, 120, 127, 652
Sau96I GGNCC 4 cut(s) 46, 159, 510, 586
SchI GAGTC 2 cut(s) 371, 590
ScrFI CCNGG 3 cut(s) 80, 132, 133
SduI GDGCHC 2 cut(s) 261, 477
SfoI GGCGCC 1 cut(s) 520
SgrAI CRCCGGYG 1 cut(s) 425
SinI GGWCC 2 cut(s) 46, 159
SmaI CCCGGG 1 cut(s) 133
SseBI AGGCCT 1 cut(s) 97
SspDI GGCGCC 1 cut(s) 518
SstI GAGCTC 1 cut(s) 261
StuI AGGCCT 1 cut(s) 97
StyD4I CCNGG 3 cut(s) 78, 130, 131
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 2 cut(s) 335, 358
TaiI ACGT 3 cut(s) 327, 608, 645
TaqI TCGA 9 cut(s) 165, 267, 393, 495, 558, 618, 624, 651, 657
TaqII GACCGA 1 cut(s) 601
TfiI GAWTC 1 cut(s) 52
TseI GCWGC 4 cut(s) 182, 342, 351, 446
TspDTI ATGAA 1 cut(s) 305
TspMI CCCGGG 1 cut(s) 131
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 2 cut(s) 46, 159
XmaI CCCGGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.