MD05G1311400.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003786
Physical Location & Seq
Forward (+)
44143698 .. 44144441
744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1311400.v1.1.491

Sequence Viewer

Length: 744 bp
ATGGCGCCGAGAGAGAAGACGGCCACCGCCGCCGTTAGGATGAACGGTAACGGAAACGTGAAGGAGGTGCATTTTAGAGGTGTGAGGAAGAGGCCGTGGGGGAGGTACGCCGCCGAGATCAGAGATCCCGGCAAGAAGAGCCGGGTCTGGCTCGGAACCTTCGATACGGCGGAGGAAGCCGCCCGGGCCTACGACGCCGCCGCCATAGAGTTCCGCGGTGCCAAGGCCAAGACCAACTTCCCTCAGCCTTCGGAAAATGTTACCCTGAATGTTACAAAAAATAATAATAATAAAATTAGTAGCGGAAGCAACAATCAGAGCCCGAGTCAGAGCAGCACGGTGGAGTCCTCCAGCCGTGAGCCGCCGGCCCTCATGGTCGATTCTTCGCCGTTGGATCTAAATCTCGCTCACGGTGTCAGCTCCGCGCCGATGAGGTTCCCCTTCCAGCACCATCAGGTTCCGGGATTCGGAGCTGTCATCGGAGTTCCGTCTTCCGCCGCGGCGGCCGCGAAGCAGGTGCTCTATTTGGACTCGGTTTTCAGAGCCGGCGCGATGAAGAATCACCAGTTTCATCCACGCATGAGATTCGATCACCCCAACTTCCACCAGCCCGATTTCCACGCTGCCGGCGGGGCCCAGAGCGACTCGGACTCGTCCTCGGTGGTCGATCTGAACCAGAACGATCTTCCTCGCGGCGGCGGAGGGTTCGATCTTAACCTCCCTCCACCACCGGACCTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

248

Amino Acids

26.6

Weight (kDa)

9.43

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 24 - 73 1.4e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 505
Acc36I ACCTGC 1 cut(s) 505
AccB1I GGYRCC 2 cut(s) 4, 218
AccII CGCG 6 cut(s) 216, 425, 500, 509, 551, 693
AclWI GGATC 2 cut(s) 119, 402
AcoI YGGCCR 2 cut(s) 21, 504
AcyI GRCGYC 2 cut(s) 5, 195
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 3 cut(s) 36, 467, 695
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 2 cut(s) 420, 473
AluI AGCT 2 cut(s) 420, 473
Alw21I GWGCWC 1 cut(s) 522
AlwI GGATC 2 cut(s) 119, 402
Ama87I CYCGRG 2 cut(s) 183, 322
AoxI GGCC 7 cut(s) 21, 92, 186, 225, 366, 504, 633
ApaI GGGCCC 1 cut(s) 637
ApeKI GCWGC 2 cut(s) 333, 623
AspLEI GCGC 3 cut(s) 7, 427, 551
AspS9I GGNCC 5 cut(s) 186, 367, 633, 634, 733
AsuC2I CCSGG 5 cut(s) 129, 143, 184, 185, 462
AsuHPI GGTGA 2 cut(s) 554, 584
AvaI CYCGRG 2 cut(s) 183, 322
AvaII GGWCC 1 cut(s) 733
BaeGI GKGCMC 1 cut(s) 637
BanI GGYRCC 2 cut(s) 4, 218
BanII GRGCYC 2 cut(s) 323, 637
BbsI GAAGAC 2 cut(s) 23, 483
Bbv12I GWGCWC 1 cut(s) 522
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 345, 610
BccI CCATC 1 cut(s) 459
BceAI ACGGC 6 cut(s) 17, 36, 79, 183, 339, 373
BcgI CGANNNNNNTGC 4 cut(s) 499, 533, 568, 602
BcnI CCSGG 5 cut(s) 129, 143, 184, 185, 462
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 505
BglI GCCNNNNNGGC 3 cut(s) 185, 503, 632
Bme1390I CCNGG 5 cut(s) 129, 143, 184, 185, 462
Bme18I GGWCC 1 cut(s) 733
BmeT110I CYCGRG 2 cut(s) 183, 322
BmgT120I GGNCC 5 cut(s) 186, 367, 633, 634, 733
BmiI GGNNCC 7 cut(s) 6, 157, 220, 437, 459, 634, 635
BmrFI CCNGG 5 cut(s) 129, 143, 184, 185, 462
BpiI GAAGAC 2 cut(s) 23, 483
BpmI CTGGAG 1 cut(s) 334
Bpu10I CCTNAGC 1 cut(s) 243
BpuMI CCSGG 5 cut(s) 129, 143, 184, 185, 462
BsaBI GATNNNNATC 1 cut(s) 399
BsaHI GRCGYC 2 cut(s) 5, 195
BsaJI CCNNGG 6 cut(s) 95, 183, 214, 222, 498, 657
BsaWI WCCGGW 1 cut(s) 730
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 467, 695
Bse118I RCCGGY 3 cut(s) 364, 545, 626
Bse1I ACTGG 1 cut(s) 565
Bse8I GATNNNNATC 1 cut(s) 399
BseDI CCNNGG 6 cut(s) 95, 183, 214, 222, 498, 657
BseGI GGATG 2 cut(s) 45, 571
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 3 cut(s) 36, 467, 695
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 1 cut(s) 565
BseSI GKGCMC 1 cut(s) 637
BseX3I CGGCCG 1 cut(s) 504
BseXI GCAGC 2 cut(s) 345, 610
Bsh1236I CGCG 6 cut(s) 216, 425, 500, 509, 551, 693
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 7 cut(s) 23, 94, 188, 227, 368, 506, 635
BshNI GGYRCC 2 cut(s) 4, 218
BsiEI CGRYCG 1 cut(s) 507
BsiHKAI GWGCWC 1 cut(s) 522
BsiHKCI CYCGRG 2 cut(s) 183, 322
BsiSI CCGG 8 cut(s) 129, 142, 184, 365, 461, 546, 627, 731
BslI CCNNNNNNNGG 3 cut(s) 36, 467, 695
BsnI GGCC 7 cut(s) 23, 94, 188, 227, 368, 506, 635
BsoBI CYCGRG 2 cut(s) 183, 322
Bsp120I GGGCCC 1 cut(s) 633
Bsp1286I GDGCHC 3 cut(s) 323, 522, 637
Bsp143I GATC 7 cut(s) 117, 124, 394, 589, 667, 682, 709
BspANI GGCC 7 cut(s) 23, 94, 188, 227, 368, 506, 635
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 6 cut(s) 216, 425, 500, 509, 551, 693
BspLI GGNNCC 7 cut(s) 6, 157, 220, 437, 459, 634, 635
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 2 cut(s) 119, 402
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 2 cut(s) 4, 218
BsrFI RCCGGY 3 cut(s) 364, 545, 626
BsrI ACTGG 1 cut(s) 565
BssAI RCCGGY 3 cut(s) 364, 545, 626
BssECI CCNNGG 6 cut(s) 95, 183, 214, 222, 498, 657
BssMI GATC 7 cut(s) 117, 124, 394, 589, 667, 682, 709
BssNI GRCGYC 2 cut(s) 5, 195
BssT1I CCWWGG 1 cut(s) 222
Bst4CI ACNGT 3 cut(s) 47, 340, 413
Bst6I CTCTTC 2 cut(s) 83, 131
BstACI GRCGYC 2 cut(s) 5, 195
BstC8I GCNNGC 3 cut(s) 366, 547, 628
BstDEI CTNAG 1 cut(s) 243
BstDSI CCRYGG 3 cut(s) 95, 214, 498
BstF5I GGATG 2 cut(s) 45, 571
BstFNI CGCG 6 cut(s) 216, 425, 500, 509, 551, 693
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 427, 551
BstKTI GATC 7 cut(s) 120, 127, 397, 592, 670, 685, 712
BstMBI GATC 7 cut(s) 117, 124, 394, 589, 667, 682, 709
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 8 cut(s) 29, 138, 176, 185, 194, 503, 506, 632
BstSCI CCNGG 5 cut(s) 127, 141, 182, 183, 460
BstSLI GKGCMC 1 cut(s) 637
BstUI CGCG 6 cut(s) 216, 425, 500, 509, 551, 693
BstV1I GCAGC 2 cut(s) 345, 610
BstV2I GAAGAC 2 cut(s) 23, 483
BstX2I RGATCY 2 cut(s) 124, 394
BstYI RGATCY 2 cut(s) 124, 394
BstZI CGGCCG 1 cut(s) 504
BsuRI GGCC 7 cut(s) 23, 94, 188, 227, 368, 506, 635
BtgI CCRYGG 3 cut(s) 95, 214, 498
BtgZI GCGATG 1 cut(s) 566
BtsCI GGATG 2 cut(s) 45, 571
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 3 cut(s) 366, 547, 628
CciNI GCGGCCGC 1 cut(s) 504
CfoI GCGC 3 cut(s) 7, 427, 551
Cfr10I RCCGGY 3 cut(s) 364, 545, 626
Cfr13I GGNCC 5 cut(s) 186, 367, 633, 634, 733
Cfr42I CCGCGG 2 cut(s) 217, 501
Cfr9I CCCGGG 1 cut(s) 183
CseI GACGC 1 cut(s) 203
Csp6I GTAC 1 cut(s) 106
CviAII CATG 2 cut(s) 373, 580
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 1 cut(s) 243
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 119, 126, 396, 591, 669, 684, 711
DpnII GATC 7 cut(s) 117, 124, 394, 589, 667, 682, 709
EaeI YGGCCR 2 cut(s) 21, 504
EagI CGGCCG 1 cut(s) 504
Eam1104I CTCTTC 2 cut(s) 83, 131
EarI CTCTTC 2 cut(s) 83, 131
EciI GGCGGA 3 cut(s) 185, 484, 714
EclXI CGGCCG 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 2 cut(s) 323, 637
Eco47I GGWCC 1 cut(s) 733
Eco52I CGGCCG 1 cut(s) 504
Eco88I CYCGRG 2 cut(s) 183, 322
EcoO109I RGGNCCY 1 cut(s) 633
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 2 cut(s) 323, 637
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 2 cut(s) 376, 583
FaiI YATR 3 cut(s) 206, 374, 581
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FatI CATG 2 cut(s) 372, 579
FauI CCCGC 1 cut(s) 623
FokI GGATG 2 cut(s) 52, 558
FriOI GRGCYC 2 cut(s) 323, 637
GlaI GCGC 3 cut(s) 6, 426, 550
GsuI CTGGAG 1 cut(s) 334
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 7 cut(s) 23, 94, 188, 227, 368, 506, 635
HapII CCGG 8 cut(s) 129, 142, 184, 365, 461, 546, 627, 731
HgaI GACGC 1 cut(s) 203
HhaI GCGC 3 cut(s) 7, 427, 551
Hin1I GRCGYC 2 cut(s) 5, 195
Hin1II CATG 2 cut(s) 376, 583
Hin6I GCGC 3 cut(s) 5, 425, 549
HinP1I GCGC 3 cut(s) 5, 425, 549
HinfI GANTC 9 cut(s) 325, 344, 380, 465, 530, 559, 585, 644, 650
HpaII CCGG 8 cut(s) 129, 142, 184, 365, 461, 546, 627, 731
HphI GGTGA 2 cut(s) 554, 584
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 4 cut(s) 55, 169, 258, 451
HpyCH4III ACNGT 3 cut(s) 47, 340, 413
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 138, 176, 185, 194, 503, 506, 632
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 57
Hsp92I GRCGYC 2 cut(s) 5, 195
Hsp92II CATG 2 cut(s) 376, 583
HspAI GCGC 3 cut(s) 5, 425, 549
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 3 cut(s) 364, 545, 626
KroNI GCCGGC 3 cut(s) 366, 547, 628
KspI CCGCGG 2 cut(s) 217, 501
Kzo9I GATC 7 cut(s) 117, 124, 394, 589, 667, 682, 709
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 2 cut(s) 425, 470
Lsp1109I GCAGC 2 cut(s) 345, 610
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 3 cut(s) 47, 259, 271
MalI GATC 7 cut(s) 119, 126, 396, 591, 669, 684, 711
MboI GATC 7 cut(s) 117, 124, 394, 589, 667, 682, 709
MboII GAAGA 7 cut(s) 28, 100, 148, 375, 483, 568, 677
MflI RGATCY 2 cut(s) 124, 394
MhlI GDGCHC 3 cut(s) 323, 522, 637
MluCI AATT 1 cut(s) 294
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 5 cut(s) 334, 353, 524, 638, 644
MmeI TCCRAC 1 cut(s) 372
MroNI GCCGGC 3 cut(s) 364, 545, 626
MseI TTAA 1 cut(s) 714
MspA1I CMGCKG 2 cut(s) 216, 500
MspI CCGG 8 cut(s) 129, 142, 184, 365, 461, 546, 627, 731
MspR9I CCNGG 5 cut(s) 129, 143, 184, 185, 462
MvnI CGCG 6 cut(s) 216, 425, 500, 509, 551, 693
MwoI GCNNNNNNNGC 8 cut(s) 29, 138, 176, 185, 194, 503, 506, 632
NaeI GCCGGC 3 cut(s) 366, 547, 628
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 5 cut(s) 129, 143, 184, 185, 462
NdeII GATC 7 cut(s) 117, 124, 394, 589, 667, 682, 709
NgoMIV GCCGGC 3 cut(s) 364, 545, 626
NlaIII CATG 2 cut(s) 376, 583
NlaIV GGNNCC 7 cut(s) 6, 157, 220, 437, 459, 634, 635
NmeAIII GCCGAG 2 cut(s) 33, 139
NotI GCGGCCGC 1 cut(s) 504
PaqCI CACCTGC 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdiI GCCGGC 3 cut(s) 366, 547, 628
PfeI GAWTC 4 cut(s) 380, 465, 559, 585
PfoI TCCNGGA 1 cut(s) 460
PleI GAGTC 5 cut(s) 333, 352, 524, 638, 644
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 5 cut(s) 333, 352, 524, 638, 644
PspN4I GGNNCC 7 cut(s) 6, 157, 220, 437, 459, 634, 635
PspOMI GGGCCC 1 cut(s) 633
PspPI GGNCC 5 cut(s) 186, 367, 633, 634, 733
PsrI GAACNNNNNNTAC 2 cut(s) 148, 180
PsuI RGATCY 2 cut(s) 124, 394
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SacII CCGCGG 2 cut(s) 217, 501
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 714
Sau3AI GATC 7 cut(s) 117, 124, 394, 589, 667, 682, 709
Sau96I GGNCC 5 cut(s) 186, 367, 633, 634, 733
SchI GAGTC 5 cut(s) 334, 353, 524, 638, 644
ScrFI CCNGG 5 cut(s) 129, 143, 184, 185, 462
SduI GDGCHC 3 cut(s) 323, 522, 637
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 2 cut(s) 217, 501
SgrBI CCGCGG 2 cut(s) 217, 501
SinI GGWCC 1 cut(s) 733
SmaI CCCGGG 1 cut(s) 185
SrfI GCCCGGGC 1 cut(s) 185
Sse9I AATT 1 cut(s) 294
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 5 cut(s) 127, 141, 182, 183, 460
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 3 cut(s) 47, 340, 413
TaiI ACGT 1 cut(s) 60
TaqI TCGA 5 cut(s) 162, 378, 588, 666, 708
TasI AATT 1 cut(s) 294
TfiI GAWTC 4 cut(s) 380, 465, 559, 585
Tru1I TTAA 1 cut(s) 714
Tru9I TTAA 1 cut(s) 714
TseI GCWGC 2 cut(s) 333, 623
TspDTI ATGAA 3 cut(s) 56, 560, 569
TspGWI ACGGA 2 cut(s) 66, 477
TspMI CCCGGG 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 733
XmaI CCCGGG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.