pycom10g24300

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003786
Physical Location & Seq
Forward (+)
26184676 .. 26185482
807 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g24300.1

Sequence Viewer

Length: 807 bp
ATGGCGCCGAGAGAGAAGACGGCGACCGCCGTTAGGGTGAACGGTAACGGAAACGTGAAGGAGGTGCATTTTAGAGGCGTGAGGAAGAGGCCGTGGGGAAGGTACGCCGCCGAGATCAGAGATCCCGGCAAGAAGAGCCGGGTCTGGCTCGGAACCTTCGACACGGCGGAGGAAGCCGCCCGGGCCTACGACAACGCCGCCAGGGATTTCCGCGGTACCAAGGCCAAGACAAACTTCCCTCTCCTTTCGGAAAATGTTGGCCTGAATATTACAAAAAATAATAATAATAATAATAATATAAATATTAATAATAAAATTAGTGGCGGAAGCAACAATCAAAGCCCGAGCCAGAGCAGCACGGTGGAGTCCTCCAGTCGTGAGCCGCCGGCTCTGATGGTCGATTCCTCGCCGTTTGATCTAAATCTCGCTCACGGTGTTAGCTCCGGAGGATTCGGCCCCGCGCCGATGAGGTTCCCCTTCCAGCACCACCAGGTTCCGGGAATCGGACCTGTCATCCGAGTCCCGGCTTCCTCCGCGGCAGCCGCGAATCACATGCTCTATTTCGACTCGGTGTTCAGAGCCGGCATGTTGAAGAACCACCAAGTTCATCAACGGCTGAGATTCGATCACCACAACTTCCACCAGCATGATTTCCAAGCCGCCGGTGGTGGTAGTTTCGCCGGTGGGGCCCAGAGCGACTCGGACTCGTCCTCGGTGGTCGATCTGAACCAGAACGATCTGCCTCGCGGCGACGGCGGAGGCGGAGGGTTCGATCTCAACCTCCCTCCTCCACCGGAACTCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

269

Amino Acids

28.85

Weight (kDa)

9.47

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 23 - 72 1.3e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 215
AccB1I GGYRCC 2 cut(s) 4, 215
AccII CGCG 5 cut(s) 213, 461, 536, 545, 747
AccIII TCCGGA 1 cut(s) 443
AclWI GGATC 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 104, 217
AfiI CCNNNNNNNGG 4 cut(s) 33, 496, 503, 523
AgsI TTSAA 1 cut(s) 592
AjnI CCWGG 2 cut(s) 200, 489
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 1 cut(s) 441
AluI AGCT 1 cut(s) 441
AlwI GGATC 1 cut(s) 116
Ama87I CYCGRG 2 cut(s) 180, 343
Aor13HI TCCGGA 1 cut(s) 443
AoxI GGCC 6 cut(s) 89, 183, 222, 259, 454, 687
ApaI GGGCCC 1 cut(s) 691
ApeKI GCWGC 2 cut(s) 354, 539
AseI ATTAAT 1 cut(s) 306
Asp718I GGTACC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 7, 463
AspS9I GGNCC 5 cut(s) 183, 455, 506, 687, 688
AsuC2I CCSGG 6 cut(s) 126, 140, 181, 182, 498, 524
AsuHPI GGTGA 2 cut(s) 49, 620
AvaI CYCGRG 2 cut(s) 180, 343
AvaII GGWCC 1 cut(s) 506
BaeGI GKGCMC 1 cut(s) 691
BanI GGYRCC 2 cut(s) 4, 215
BanII GRGCYC 1 cut(s) 691
BbsI GAAGAC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 366, 551
BccI CCATC 1 cut(s) 388
BceAI ACGGC 7 cut(s) 14, 36, 76, 180, 394, 629, 769
BcgI CGANNNNNNTGC 2 cut(s) 535, 569
BciT130I CCWGG 2 cut(s) 202, 491
BcnI CCSGG 6 cut(s) 126, 140, 181, 182, 498, 524
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 2 cut(s) 182, 686
Bme1390I CCNGG 8 cut(s) 126, 140, 181, 182, 202, 491, 498, 524
Bme18I GGWCC 1 cut(s) 506
BmeT110I CYCGRG 2 cut(s) 180, 343
BmgT120I GGNCC 5 cut(s) 183, 455, 506, 687, 688
BmiI GGNNCC 8 cut(s) 6, 154, 217, 457, 473, 495, 688, 689
BmrFI CCNGG 8 cut(s) 126, 140, 181, 182, 202, 491, 498, 524
BpiI GAAGAC 1 cut(s) 23
BpmI CTGGAG 1 cut(s) 355
BpuMI CCSGG 6 cut(s) 126, 140, 181, 182, 498, 524
BsaBI GATNNNNATC 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 7 cut(s) 92, 180, 201, 211, 219, 534, 711
BsaWI WCCGGW 2 cut(s) 443, 793
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 496, 503, 523
Bse118I RCCGGY 4 cut(s) 385, 581, 662, 680
Bse1I ACTGG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 420
BseAI TCCGGA 1 cut(s) 443
BseBI CCWGG 2 cut(s) 202, 491
BseDI CCNNGG 7 cut(s) 92, 180, 201, 211, 219, 534, 711
BseGI GGATG 1 cut(s) 513
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 4 cut(s) 33, 496, 503, 523
BseMII CTCAG 1 cut(s) 608
BseNI ACTGG 1 cut(s) 372
BseRI GAGGAG 1 cut(s) 777
BseSI GKGCMC 1 cut(s) 691
BseXI GCAGC 2 cut(s) 366, 551
Bsh1236I CGCG 5 cut(s) 213, 461, 536, 545, 747
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 6 cut(s) 91, 185, 224, 261, 456, 689
BshNI GGYRCC 2 cut(s) 4, 215
BsiEI CGRYCG 1 cut(s) 27
BsiHKCI CYCGRG 2 cut(s) 180, 343
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 4 cut(s) 33, 496, 503, 523
BsmFI GGGAC 1 cut(s) 506
BsnI GGCC 6 cut(s) 91, 185, 224, 261, 456, 689
BsoBI CYCGRG 2 cut(s) 180, 343
Bsp120I GGGCCC 1 cut(s) 687
Bsp1286I GDGCHC 1 cut(s) 691
Bsp13I TCCGGA 1 cut(s) 443
Bsp143I GATC 7 cut(s) 114, 121, 415, 625, 721, 736, 772
BspANI GGCC 6 cut(s) 91, 185, 224, 261, 456, 689
BspCNI CTCAG 1 cut(s) 609
BspEI TCCGGA 1 cut(s) 443
BspFNI CGCG 5 cut(s) 213, 461, 536, 545, 747
BspLI GGNNCC 8 cut(s) 6, 154, 217, 457, 473, 495, 688, 689
BspPI GGATC 1 cut(s) 116
BspQI GCTCTTC 1 cut(s) 128
BspT107I GGYRCC 2 cut(s) 4, 215
BsrFI RCCGGY 4 cut(s) 385, 581, 662, 680
BsrI ACTGG 1 cut(s) 372
BssAI RCCGGY 4 cut(s) 385, 581, 662, 680
BssECI CCNNGG 7 cut(s) 92, 180, 201, 211, 219, 534, 711
BssMI GATC 7 cut(s) 114, 121, 415, 625, 721, 736, 772
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 2 cut(s) 202, 491
Bst4CI ACNGT 3 cut(s) 44, 361, 434
Bst6I CTCTTC 2 cut(s) 80, 128
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 387, 583
BstDEI CTNAG 1 cut(s) 617
BstDSI CCRYGG 3 cut(s) 92, 211, 534
BstF5I GGATG 1 cut(s) 513
BstFNI CGCG 5 cut(s) 213, 461, 536, 545, 747
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 463
BstKTI GATC 7 cut(s) 117, 124, 418, 628, 724, 739, 775
BstMBI GATC 7 cut(s) 114, 121, 415, 625, 721, 736, 772
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 8 cut(s) 135, 173, 182, 354, 533, 542, 686, 753
BstNI CCWGG 2 cut(s) 202, 491
BstNSI RCATGY 2 cut(s) 556, 589
BstSCI CCNGG 8 cut(s) 124, 138, 179, 180, 200, 489, 496, 522
BstSLI GKGCMC 1 cut(s) 691
BstUI CGCG 5 cut(s) 213, 461, 536, 545, 747
BstV1I GCAGC 2 cut(s) 366, 551
BstV2I GAAGAC 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 6 cut(s) 91, 185, 224, 261, 456, 689
BtgI CCRYGG 3 cut(s) 92, 211, 534
BtsCI GGATG 1 cut(s) 513
Cac8I GCNNGC 2 cut(s) 387, 583
CfoI GCGC 2 cut(s) 7, 463
Cfr10I RCCGGY 4 cut(s) 385, 581, 662, 680
Cfr13I GGNCC 5 cut(s) 183, 455, 506, 687, 688
Cfr42I CCGCGG 2 cut(s) 214, 537
Cfr9I CCCGGG 1 cut(s) 180
CsiI ACCWGGT 1 cut(s) 489
Csp6I GTAC 2 cut(s) 103, 216
CviAII CATG 3 cut(s) 553, 586, 647
CviQI GTAC 2 cut(s) 103, 216
DdeI CTNAG 1 cut(s) 617
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 116, 123, 417, 627, 723, 738, 774
DpnII GATC 7 cut(s) 114, 121, 415, 625, 721, 736, 772
Eam1104I CTCTTC 2 cut(s) 80, 128
EarI CTCTTC 2 cut(s) 80, 128
EciI GGCGGA 4 cut(s) 182, 339, 771, 777
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 691
Eco47I GGWCC 1 cut(s) 506
Eco88I CYCGRG 2 cut(s) 180, 343
EcoO109I RGGNCCY 1 cut(s) 687
EcoRII CCWGG 2 cut(s) 200, 489
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 691
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 3 cut(s) 556, 589, 650
FaiI YATR 4 cut(s) 299, 554, 587, 648
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 506
FatI CATG 3 cut(s) 552, 585, 646
FauI CCCGC 1 cut(s) 466
FokI GGATG 1 cut(s) 500
FriOI GRGCYC 1 cut(s) 691
GlaI GCGC 2 cut(s) 6, 462
GsuI CTGGAG 1 cut(s) 355
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 91, 185, 224, 261, 456, 689
HhaI GCGC 2 cut(s) 7, 463
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 556, 589, 650
Hin6I GCGC 2 cut(s) 5, 461
HinP1I GCGC 2 cut(s) 5, 461
HphI GGTGA 2 cut(s) 49, 620
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 9 cut(s) 119, 152, 250, 393, 506, 518, 578, 703, 726
Hpy188III TCNNGA 2 cut(s) 377, 444
Hpy8I GTNNAC 1 cut(s) 40
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 4 cut(s) 52, 93, 166, 487
HpyCH4III ACNGT 3 cut(s) 44, 361, 434
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 1 cut(s) 67
HpyF10VI GCNNNNNNNGC 8 cut(s) 135, 173, 182, 354, 533, 542, 686, 753
HpyF3I CTNAG 1 cut(s) 617
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 556, 589, 650
HspAI GCGC 2 cut(s) 5, 461
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 443
KpnI GGTACC 1 cut(s) 219
KroI GCCGGC 2 cut(s) 385, 581
KroNI GCCGGC 2 cut(s) 387, 583
KspI CCGCGG 2 cut(s) 214, 537
Kzo9I GATC 7 cut(s) 114, 121, 415, 625, 721, 736, 772
LguI GCTCTTC 1 cut(s) 128
LmnI GCTCC 1 cut(s) 446
Lsp1109I GCAGC 2 cut(s) 366, 551
MabI ACCWGGT 1 cut(s) 489
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 44
MalI GATC 7 cut(s) 116, 123, 417, 627, 723, 738, 774
MboI GATC 7 cut(s) 114, 121, 415, 625, 721, 736, 772
MboII GAAGA 4 cut(s) 28, 97, 145, 604
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 1 cut(s) 315
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 5 cut(s) 374, 528, 560, 692, 698
MroI TCCGGA 1 cut(s) 443
MroNI GCCGGC 2 cut(s) 385, 581
MseI TTAA 1 cut(s) 306
MslI CAYNNNNRTG 1 cut(s) 645
MspA1I CMGCKG 2 cut(s) 213, 536
MspR9I CCNGG 8 cut(s) 126, 140, 181, 182, 202, 491, 498, 524
MvaI CCWGG 2 cut(s) 202, 491
MvnI CGCG 5 cut(s) 213, 461, 536, 545, 747
MwoI GCNNNNNNNGC 8 cut(s) 135, 173, 182, 354, 533, 542, 686, 753
NaeI GCCGGC 2 cut(s) 387, 583
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 6 cut(s) 126, 140, 181, 182, 498, 524
NdeII GATC 7 cut(s) 114, 121, 415, 625, 721, 736, 772
NgoMIV GCCGGC 2 cut(s) 385, 581
NlaIII CATG 3 cut(s) 556, 589, 650
NlaIV GGNNCC 8 cut(s) 6, 154, 217, 457, 473, 495, 688, 689
NmeAIII GCCGAG 2 cut(s) 33, 136
NspI RCATGY 2 cut(s) 556, 589
PciSI GCTCTTC 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PdiI GCCGGC 2 cut(s) 387, 583
PfeI GAWTC 5 cut(s) 401, 450, 501, 547, 621
PfoI TCCNGGA 1 cut(s) 496
PleI GAGTC 5 cut(s) 373, 527, 560, 692, 698
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 5 cut(s) 373, 527, 560, 692, 698
PshBI ATTAAT 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 200, 489
PspGI CCWGG 2 cut(s) 200, 489
PspN4I GGNNCC 8 cut(s) 6, 154, 217, 457, 473, 495, 688, 689
PspOMI GGGCCC 1 cut(s) 687
PspPI GGNCC 5 cut(s) 183, 455, 506, 687, 688
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 2 cut(s) 104, 217
RsaNI GTAC 2 cut(s) 103, 216
RseI CAYNNNNRTG 1 cut(s) 645
SacII CCGCGG 2 cut(s) 214, 537
SapI GCTCTTC 1 cut(s) 128
SaqAI TTAA 1 cut(s) 306
Sau3AI GATC 7 cut(s) 114, 121, 415, 625, 721, 736, 772
Sau96I GGNCC 5 cut(s) 183, 455, 506, 687, 688
SchI GAGTC 5 cut(s) 374, 528, 560, 692, 698
ScrFI CCNGG 8 cut(s) 126, 140, 181, 182, 202, 491, 498, 524
SduI GDGCHC 1 cut(s) 691
SetI ASST 9 cut(s) 57, 66, 104, 158, 443, 473, 495, 511, 783
SexAI ACCWGGT 1 cut(s) 489
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 2 cut(s) 214, 537
SgrAI CRCCGGYG 2 cut(s) 662, 680
SgrBI CCGCGG 2 cut(s) 214, 537
SinI GGWCC 1 cut(s) 506
SmaI CCCGGG 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 645
SrfI GCCCGGGC 1 cut(s) 182
Sse9I AATT 1 cut(s) 315
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 2 cut(s) 268, 304
StyD4I CCNGG 8 cut(s) 124, 138, 179, 180, 200, 489, 496, 522
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 3 cut(s) 44, 361, 434
TaiI ACGT 1 cut(s) 57
TaqI TCGA 6 cut(s) 159, 399, 564, 624, 720, 771
TasI AATT 1 cut(s) 315
TauI GCSGC 8 cut(s) 110, 179, 200, 385, 539, 545, 662, 750
TfiI GAWTC 5 cut(s) 401, 450, 501, 547, 621
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseI GCWGC 2 cut(s) 354, 539
TspDTI ATGAA 1 cut(s) 596
TspGWI ACGGA 1 cut(s) 63
TspMI CCCGGG 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 506
VspI ATTAAT 1 cut(s) 306
XceI RCATGY 2 cut(s) 556, 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XmaI CCCGGG 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.