Rh5CG254800

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003786
Physical Location & Seq
Forward (+)
27009011 .. 27011902
2892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG254800.1

Sequence Viewer

Length: 765 bp
ATGGCTCCGAAAACGGCAGCAAAATCCGGCTCCGGGACCCAGAACCCGAAGCCGAACTCGCTCGAGATCCGTTATCGGGGCGTCAGGAAGAGGCCTTGGGGTCGCTACGCTGCCGAGATCAGGGATCCTGGCAAGAAGACGAGGGTCTGGCTCGGCACGTTCGATACGGCTGAGGAGGCGGCGCGTGCGTACGACAAGGCGGCGCGGGAGTACCGTGGAGCTAAGGCTAAGACCAACTTCCCAACGCCGGCCGAGCTTCACGCGAACACAAACAACCGCGACCGCGGCAACAATAACAGCGGCGGCAGACAGAACCATCATTCCCCGCCCGCGGCTCAGGCGCGCAGCCCCAGCCAGACCAGCACCGTGGACTCCTCGTCCCCGCCGGCGCAGGCGCCTCCGGCCCTGGACCTCACCCTCGCAGCGCCCCGTTTCGCGGCGGCGGCGGCGGCCAACGCGGGCTACTTCACCGCCGCGAGTGCCTACCACGCGCTCCCCCTCGGGCGACCCATCTTTTTCTTCGACGCGTTCGCCAGGACCGACAACGTGGTGACGACGACGACGGGTCTCCATCGCCCCCTCGCCGCCCGCGAAATGGGCATACTTGACAAAAGCGTCAAGGCGGCCGCCTCACCGTCCGGCGGTTCCCACTCCGGCCTCAGCGACTCGGACTCCTCCACCGTCGTGGATTTCGCTCACTCCCCCGCCAAGAAAGGACCGCTCGATCTCGACCTCAACCTCCCCCCTCCACCGGAGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009873 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010104 GO:0010105 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016604 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032870 GO:0033993 GO:0035556 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070297 GO:0070298 GO:0070887 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

254

Amino Acids

26.82

Weight (kDa)

9.98

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 24 - 73 2e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 394
AccBSI CCGCTC 1 cut(s) 721
AclWI GGATC 3 cut(s) 61, 119, 132
AcoI YGGCCR 3 cut(s) 249, 450, 624
AcyI GRCGYC 2 cut(s) 81, 395
AfaI GTAC 2 cut(s) 191, 212
AfiI CCNNNNNNNGG 9 cut(s) 33, 76, 120, 247, 331, 436, 595, 641, 751
AflIII ACRYGT 1 cut(s) 525
AhdI GACNNNNNGTC 2 cut(s) 376, 564
AjnI CCWGG 3 cut(s) 127, 405, 533
AleI CACNNNNGTG 1 cut(s) 683
AloI GAACNNNNNNTCC 2 cut(s) 305, 337
AluBI AGCT 2 cut(s) 221, 256
AluI AGCT 2 cut(s) 221, 256
Alw26I GTCTC 1 cut(s) 572
AlwI GGATC 3 cut(s) 61, 119, 132
Ama87I CYCGRG 2 cut(s) 62, 500
AoxI GGCC 6 cut(s) 92, 249, 402, 450, 624, 655
ApeKI GCWGC 4 cut(s) 17, 110, 345, 422
AspLEI GCGC 8 cut(s) 184, 205, 343, 345, 391, 397, 427, 493
AspS9I GGNCC 5 cut(s) 36, 403, 409, 537, 716
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 4 cut(s) 406, 460, 562, 624
AvaI CYCGRG 2 cut(s) 62, 500
AvaII GGWCC 4 cut(s) 36, 409, 537, 716
BamHI GGATCC 1 cut(s) 124
BanI GGYRCC 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 143
BbvCI CCTCAGC 2 cut(s) 171, 659
BbvI GCAGC 4 cut(s) 29, 97, 357, 434
BccI CCATC 3 cut(s) 324, 518, 579
BceAI ACGGC 2 cut(s) 30, 183
BciT130I CCWGG 3 cut(s) 129, 407, 535
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 572
BfoI RGCGCY 2 cut(s) 398, 428
Bme1390I CCNGG 4 cut(s) 34, 129, 407, 535
Bme18I GGWCC 4 cut(s) 36, 409, 537, 716
BmeRI GACNNNNNGTC 2 cut(s) 376, 564
BmeT110I CYCGRG 2 cut(s) 62, 500
BmgT120I GGNCC 5 cut(s) 36, 403, 409, 537, 716
BmiI GGNNCC 7 cut(s) 6, 31, 37, 38, 126, 396, 646
BmrFI CCNGG 4 cut(s) 34, 129, 407, 535
BoxI GACNNNNGTC 1 cut(s) 143
BpiI GAAGAC 1 cut(s) 143
Bpu10I CCTNAGC 4 cut(s) 171, 222, 336, 659
BpuMI CCSGG 1 cut(s) 34
BsaHI GRCGYC 2 cut(s) 81, 395
BsaI GGTCTC 1 cut(s) 572
BsaJI CCNNGG 7 cut(s) 95, 214, 283, 330, 366, 405, 499
BsaWI WCCGGW 1 cut(s) 751
BsaXI ACNNNNNCTCC 2 cut(s) 656, 686
Bsc4I CCNNNNNNNGG 9 cut(s) 33, 76, 120, 247, 331, 436, 595, 641, 751
Bse118I RCCGGY 2 cut(s) 247, 385
BseBI CCWGG 3 cut(s) 129, 407, 535
BseDI CCNNGG 7 cut(s) 95, 214, 283, 330, 366, 405, 499
BseLI CCNNNNNNNGG 9 cut(s) 33, 76, 120, 247, 331, 436, 595, 641, 751
BseMII CTCAG 3 cut(s) 162, 350, 673
BsePI GCGCGC 1 cut(s) 341
BseRI GAGGAG 3 cut(s) 188, 364, 664
BseX3I CGGCCG 2 cut(s) 249, 624
BseXI GCAGC 4 cut(s) 29, 97, 357, 434
BseYI CCCAGC 1 cut(s) 350
Bsh1285I CGRYCG 3 cut(s) 252, 283, 627
BshFI GGCC 6 cut(s) 94, 251, 404, 452, 626, 657
BshNI GGYRCC 1 cut(s) 394
BsiEI CGRYCG 3 cut(s) 252, 283, 627
BsiHKCI CYCGRG 2 cut(s) 62, 500
BsiSI CCGG 8 cut(s) 27, 33, 248, 386, 401, 639, 654, 752
BsiWI CGTACG 1 cut(s) 189
BslFI GGGAC 2 cut(s) 49, 364
BslI CCNNNNNNNGG 9 cut(s) 33, 76, 120, 247, 331, 436, 595, 641, 751
BsmAI GTCTC 1 cut(s) 572
BsmFI GGGAC 2 cut(s) 49, 364
BsnI GGCC 6 cut(s) 94, 251, 404, 452, 626, 657
Bso31I GGTCTC 1 cut(s) 572
BsoBI CYCGRG 2 cut(s) 62, 500
Bsp143I GATC 4 cut(s) 66, 117, 124, 724
BspANI GGCC 6 cut(s) 94, 251, 404, 452, 626, 657
BspCNI CTCAG 3 cut(s) 163, 349, 672
BspLI GGNNCC 7 cut(s) 6, 31, 37, 38, 126, 396, 646
BspPI GGATC 3 cut(s) 61, 119, 132
BspT107I GGYRCC 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 572
BsrBI CCGCTC 1 cut(s) 721
BsrFI RCCGGY 2 cut(s) 247, 385
BssAI RCCGGY 2 cut(s) 247, 385
BssECI CCNNGG 7 cut(s) 95, 214, 283, 330, 366, 405, 499
BssHII GCGCGC 1 cut(s) 341
BssMI GATC 4 cut(s) 66, 117, 124, 724
BssNI GRCGYC 2 cut(s) 81, 395
BssT1I CCWWGG 1 cut(s) 95
Bst2UI CCWGG 3 cut(s) 129, 407, 535
Bst4CI ACNGT 4 cut(s) 215, 367, 636, 682
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 2 cut(s) 81, 395
BstC8I GCNNGC 8 cut(s) 186, 249, 330, 343, 387, 393, 460, 589
BstDEI CTNAG 5 cut(s) 171, 222, 228, 336, 659
BstDSI CCRYGG 4 cut(s) 214, 283, 330, 366
BstH2I RGCGCY 2 cut(s) 398, 428
BstHHI GCGC 8 cut(s) 184, 205, 343, 345, 391, 397, 427, 493
BstKTI GATC 4 cut(s) 69, 120, 127, 727
BstMAI GTCTC 1 cut(s) 572
BstMBI GATC 4 cut(s) 66, 117, 124, 724
BstMCI CGRYCG 3 cut(s) 252, 283, 627
BstNI CCWGG 3 cut(s) 129, 407, 535
BstPAI GACNNNNGTC 1 cut(s) 143
BstSCI CCNGG 4 cut(s) 32, 127, 405, 533
BstV1I GCAGC 4 cut(s) 29, 97, 357, 434
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 2 cut(s) 66, 124
BstXI CCANNNNNNTGG 2 cut(s) 367, 685
BstYI RGATCY 2 cut(s) 66, 124
BstZI CGGCCG 2 cut(s) 249, 624
BsuRI GGCC 6 cut(s) 94, 251, 404, 452, 626, 657
BtgI CCRYGG 4 cut(s) 214, 283, 330, 366
BtgZI GCGATG 1 cut(s) 557
Cac8I GCNNGC 8 cut(s) 186, 249, 330, 343, 387, 393, 460, 589
CciNI GCGGCCGC 1 cut(s) 624
CfoI GCGC 8 cut(s) 184, 205, 343, 345, 391, 397, 427, 493
Cfr10I RCCGGY 2 cut(s) 247, 385
Cfr13I GGNCC 5 cut(s) 36, 403, 409, 537, 716
Cfr42I CCGCGG 2 cut(s) 286, 333
CseI GACGC 3 cut(s) 70, 533, 604
Csp6I GTAC 2 cut(s) 190, 211
CviQI GTAC 2 cut(s) 190, 211
DdeI CTNAG 5 cut(s) 171, 222, 228, 336, 659
DinI GGCGCC 1 cut(s) 396
DpnI GATC 4 cut(s) 68, 119, 126, 726
DpnII GATC 4 cut(s) 66, 117, 124, 724
DrdI GACNNNNNNGTC 1 cut(s) 614
DriI GACNNNNNGTC 2 cut(s) 376, 564
DseDI GACNNNNNNGTC 1 cut(s) 614
EaeI YGGCCR 3 cut(s) 249, 450, 624
EagI CGGCCG 2 cut(s) 249, 624
Eam1104I CTCTTC 1 cut(s) 83
Eam1105I GACNNNNNGTC 2 cut(s) 376, 564
EarI CTCTTC 1 cut(s) 83
EclXI CGGCCG 2 cut(s) 249, 624
Eco130I CCWWGG 1 cut(s) 95
Eco147I AGGCCT 1 cut(s) 94
Eco31I GGTCTC 1 cut(s) 572
Eco47I GGWCC 4 cut(s) 36, 409, 537, 716
Eco52I CGGCCG 2 cut(s) 249, 624
Eco88I CYCGRG 2 cut(s) 62, 500
EcoO109I RGGNCCY 1 cut(s) 36
EcoRII CCWGG 3 cut(s) 127, 405, 533
EcoT14I CCWWGG 1 cut(s) 95
EgeI GGCGCC 1 cut(s) 396
EheI GGCGCC 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 95
FaiI YATR 1 cut(s) 602
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 2 cut(s) 49, 364
FauI CCCGC 7 cut(s) 198, 333, 337, 390, 451, 596, 712
GlaI GCGC 8 cut(s) 183, 204, 342, 344, 390, 396, 426, 492
GsaI CCCAGC 1 cut(s) 354
HaeII RGCGCY 2 cut(s) 398, 428
HaeIII GGCC 6 cut(s) 94, 251, 404, 452, 626, 657
HapII CCGG 8 cut(s) 27, 33, 248, 386, 401, 639, 654, 752
HgaI GACGC 3 cut(s) 70, 533, 604
HhaI GCGC 8 cut(s) 184, 205, 343, 345, 391, 397, 427, 493
Hin1I GRCGYC 2 cut(s) 81, 395
Hin6I GCGC 8 cut(s) 182, 203, 341, 343, 389, 395, 425, 491
HinP1I GCGC 8 cut(s) 182, 203, 341, 343, 389, 395, 425, 491
HinfI GANTC 3 cut(s) 371, 665, 671
HpaII CCGG 8 cut(s) 27, 33, 248, 386, 401, 639, 654, 752
HphI GGTGA 4 cut(s) 406, 460, 562, 624
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 2 cut(s) 9, 670
Hpy188III TCNNGA 3 cut(s) 64, 85, 728
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 5 cut(s) 527, 559, 562, 565, 686
HpyCH4III ACNGT 4 cut(s) 215, 367, 636, 682
HpyCH4IV ACGT 2 cut(s) 158, 546
HpyF3I CTNAG 5 cut(s) 171, 222, 228, 336, 659
HpySE526I ACGT 2 cut(s) 158, 546
Hsp92I GRCGYC 2 cut(s) 81, 395
HspAI GCGC 8 cut(s) 182, 203, 341, 343, 389, 395, 425, 491
KasI GGCGCC 1 cut(s) 394
KflI GGGWCCC 1 cut(s) 36
KroI GCCGGC 2 cut(s) 247, 385
KroNI GCCGGC 2 cut(s) 249, 387
KspI CCGCGG 2 cut(s) 286, 333
Kzo9I GATC 4 cut(s) 66, 117, 124, 724
LmnI GCTCC 4 cut(s) 10, 35, 218, 498
Lsp1109I GCAGC 4 cut(s) 29, 97, 357, 434
MaeII ACGT 2 cut(s) 158, 546
MaeIII GTNAC 1 cut(s) 550
MalI GATC 4 cut(s) 68, 119, 126, 726
MbiI CCGCTC 1 cut(s) 721
MboI GATC 4 cut(s) 66, 117, 124, 724
MboII GAAGA 3 cut(s) 100, 148, 511
MflI RGATCY 2 cut(s) 66, 124
MluI ACGCGT 1 cut(s) 525
Mly113I GGCGCC 1 cut(s) 395
MlyI GAGTC 3 cut(s) 365, 659, 665
MreI CGCCGGCG 1 cut(s) 385
MroNI GCCGGC 2 cut(s) 247, 385
MslI CAYNNNNRTG 1 cut(s) 683
MspA1I CMGCKG 3 cut(s) 285, 300, 332
MspI CCGG 8 cut(s) 27, 33, 248, 386, 401, 639, 654, 752
MspR9I CCNGG 4 cut(s) 34, 129, 407, 535
MvaI CCWGG 3 cut(s) 129, 407, 535
NaeI GCCGGC 2 cut(s) 249, 387
NarI GGCGCC 1 cut(s) 395
NciI CCSGG 1 cut(s) 34
NdeII GATC 4 cut(s) 66, 117, 124, 724
NgoMIV GCCGGC 2 cut(s) 247, 385
NlaIV GGNNCC 7 cut(s) 6, 31, 37, 38, 126, 396, 646
NmeAIII GCCGAG 3 cut(s) 132, 139, 277
NmuCI GTSAC 1 cut(s) 550
NotI GCGGCCGC 1 cut(s) 624
OliI CACNNNNGTG 1 cut(s) 683
PaeR7I CTCGAG 1 cut(s) 62
PauI GCGCGC 1 cut(s) 341
PceI AGGCCT 1 cut(s) 94
PcsI WCGNNNNNNNCGW 3 cut(s) 159, 537, 588
PdiI GCCGGC 2 cut(s) 249, 387
Pfl23II CGTACG 1 cut(s) 189
PfoI TCCNGGA 1 cut(s) 32
PleI GAGTC 3 cut(s) 365, 659, 665
PluTI GGCGCC 1 cut(s) 398
PpsI GAGTC 3 cut(s) 365, 659, 665
PpuMI RGGWCCY 1 cut(s) 36
PshAI GACNNNNGTC 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 36
Psp6I CCWGG 3 cut(s) 127, 405, 533
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 3 cut(s) 127, 405, 533
PspLI CGTACG 1 cut(s) 189
PspN4I GGNNCC 7 cut(s) 6, 31, 37, 38, 126, 396, 646
PspPI GGNCC 5 cut(s) 36, 403, 409, 537, 716
PspPPI RGGWCCY 1 cut(s) 36
PsuI RGATCY 2 cut(s) 66, 124
PteI GCGCGC 1 cut(s) 341
RsaI GTAC 2 cut(s) 191, 212
RsaNI GTAC 2 cut(s) 190, 211
RseI CAYNNNNRTG 1 cut(s) 683
SacII CCGCGG 2 cut(s) 286, 333
Sau3AI GATC 4 cut(s) 66, 117, 124, 724
Sau96I GGNCC 5 cut(s) 36, 403, 409, 537, 716
SchI GAGTC 3 cut(s) 365, 659, 665
ScrFI CCNGG 4 cut(s) 34, 129, 407, 535
SetI ASST 7 cut(s) 161, 223, 258, 414, 549, 735, 741
SfoI GGCGCC 1 cut(s) 396
Sfr274I CTCGAG 1 cut(s) 62
Sfr303I CCGCGG 2 cut(s) 286, 333
SgrAI CRCCGGYG 1 cut(s) 385
SgrBI CCGCGG 2 cut(s) 286, 333
SinI GGWCC 4 cut(s) 36, 409, 537, 716
SlaI CTCGAG 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 683
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SseBI AGGCCT 1 cut(s) 94
SspDI GGCGCC 1 cut(s) 394
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 4 cut(s) 32, 127, 405, 533
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 4 cut(s) 215, 367, 636, 682
TaiI ACGT 2 cut(s) 161, 549
TaqI TCGA 5 cut(s) 63, 162, 522, 723, 729
TaqII GACCGA 1 cut(s) 554
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 4 cut(s) 17, 110, 345, 422
Tsp45I GTSAC 1 cut(s) 550
TspGWI ACGGA 1 cut(s) 59
VpaK11BI GGWCC 4 cut(s) 36, 409, 537, 716
XhoI CTCGAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.