FvH4_3g35480

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003332
Physical Location & Seq
Reverse (-)
30516822 .. 30521625
4804 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35480.t2

Sequence Viewer

Length: 1263 bp
ATGGGAATTCCGAGCGACGACGTCGTCGTTATCCAGAAAGCAAAACGGCCCGGCGAGCCTTGCGTCATCACCGTTAATTGCCCGGATAAGACCGGCCTGGGTTGCGATATCTGCAGAATCATCCTCGATTTCGGGCTCTACATCAAAAAAGCAGATTTTTCGACTGATGGGATATGGTGCTACCTTGTATTATGGGTGGTTCCAAATTCAAGCTCAACAGCGGTGAAATGGTCGAATTTGGAGAAGCAGCTCCTATCCGTGTGCCCGTCATGTTCTGTTCCGTATTACTTCTACCCCCAGCCTACATGTTCGGCCCCTGCAGTTTATCTATTAAAGTTTCTTTGCTCTGACCGTTATGGATTATTGCATGATGTCACTGAAATTCTGTCTGATCTTGAGCTTGCGATTCAAATGGTGAAAGTGACAACAACACCAGATGACAGGGTCTTGGACTTGTTCTTCATAACAGATAACATGGAGCTTCTGCACACAAAGGAGCGGCAAGATAAGACATTGAAACAGTTGCAAGCTGTGTTGGGTGAGTCGTGTACTAGTTGCGAACTCCAGTTGGTAGGTCCTGAGTATGATCCCCACCATGGCATCTCTTCTCTGTCTCCTGTAGTAGCCGAAGAGCTATTTAGATGTGAGCTTTTGGATAAAGAAATCCGTTCTCAAGCTCTTAGTCCAGATATGGCTAAACTAAAGAACGCCAATGTGACAATAGACAACTCATTGAGCCCGGCACACACATTACTTCAGATCCACTGTGTTGATCACAAGGGTCTCTTTTATGACATTATGAGAACTTTGAAAGACAGCAACATCAAGATAGCTTATGGTCGATTTTCTCCAGCTACAAAAGGGTATCGTGATTTAGACCTTTTTATTCAGCGAAAGGACGGGAAAAAGATTCAGGATCCAGAGGAGCAGAGTGTGTTGTGTTTGAATTTAAAGGTGGAGATGCTTCACCCACTGCGTGTTATTATTGCAAACCGAGGGCCTGATACAGAATTATTGGTTGCTAATCCAGTTGAGAAATCTGGAAAGGGGCGACCACTAGTTTTCTTTGATGTTACACGGGCACTAAAAGCACTGAAGATCTGCATTTTCTCGGCTGAAATTGGAAGGAGGATCTCAGCAGTAGATCGTGAATGGGAGGTGTATAGTTTTCTGTTGGAGGAGAACACCAAATTTCAATTCTCAAATATGGTAGCTAAGAATCAGATTGTAGATAGAGTTAGACGAACACTGATGGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

421

Amino Acids

47.45

Weight (kDa)

6.28

Isoelectric Point (pI)

37.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACR10_N PF24914 11 - 89 4.5e-40 ACT domain-containing protein ACR10, N-terminal
ACT PF01842 110 - 170 8.9e-07 ACT domain
ACT_ACR9_3rd PF24931 238 - 335 5.6e-47 ACR9-like, ACT-like domain
ACT_ACR9_C PF24926 336 - 419 2.9e-35 ACT domain-containing protein ACR9, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 23
AatII GACGTC 1 cut(s) 24
AccBSI CCGCTC 1 cut(s) 499
AciI CCGC 2 cut(s) 221, 499
AclWI GGATC 5 cut(s) 581, 754, 911, 924, 1139
AcsI RAATTY 6 cut(s) 6, 205, 235, 381, 946, 1190
AcuI CTGAAG 2 cut(s) 740, 1115
AcyI GRCGYC 1 cut(s) 21
AdeI CACNNNGTG 1 cut(s) 977
AfaI GTAC 1 cut(s) 550
AfiI CCNNNNNNNGG 1 cut(s) 596
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 6 cut(s) 210, 410, 517, 811, 946, 1196
AhlI ACTAGT 2 cut(s) 551, 1057
AjnI CCWGG 1 cut(s) 96
AjuI GAANNNNNNNTTGG 2 cut(s) 1181, 1213
Alw26I GTCTC 2 cut(s) 618, 788
AlwI GGATC 5 cut(s) 581, 754, 911, 924, 1139
AoxI GGCC 4 cut(s) 47, 94, 312, 998
ApeKI GCWGC 1 cut(s) 247
ApoI RAATTY 6 cut(s) 6, 205, 235, 381, 946, 1190
AspS9I GGNCC 4 cut(s) 48, 313, 575, 998
AsuC2I CCSGG 3 cut(s) 51, 83, 740
AsuHPI GGTGA 5 cut(s) 61, 235, 427, 551, 959
AvaII GGWCC 1 cut(s) 575
BaeGI GKGCMC 2 cut(s) 266, 1084
BamHI GGATCC 1 cut(s) 916
BanII GRGCYC 2 cut(s) 138, 740
BbvI GCAGC 1 cut(s) 259
BccI CCATC 3 cut(s) 161, 1246, 1251
BceAI ACGGC 1 cut(s) 62
BcgI CGANNNNNNTGC 2 cut(s) 141, 175
BciT130I CCWGG 1 cut(s) 98
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 3 cut(s) 51, 83, 740
BcoDI GTCTC 2 cut(s) 618, 788
BcuI ACTAGT 2 cut(s) 551, 1057
BfaI CTAG 2 cut(s) 552, 1058
BfmI CTRYAG 3 cut(s) 112, 318, 618
BglII AGATCT 1 cut(s) 1098
BisI GCNGC 2 cut(s) 248, 500
BlsI GCNGC 2 cut(s) 249, 501
Bme1390I CCNGG 4 cut(s) 51, 83, 98, 740
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 4 cut(s) 48, 313, 575, 998
BmiI GGNNCC 3 cut(s) 201, 315, 918
BmrFI CCNGG 4 cut(s) 51, 83, 98, 740
BmsI GCATC 2 cut(s) 609, 951
BpmI CTGGAG 2 cut(s) 548, 834
BpuEI CTTGAG 2 cut(s) 416, 657
BpuMI CCSGG 3 cut(s) 51, 83, 740
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 788
BsaJI CCNNGG 3 cut(s) 97, 595, 994
Bsc4I CCNNNNNNNGG 1 cut(s) 596
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 565, 1028
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 3 cut(s) 97, 595, 994
BseGI GGATG 2 cut(s) 120, 1262
BseLI CCNNNNNNNGG 1 cut(s) 596
BseMII CTCAG 2 cut(s) 570, 1149
BseNI ACTGG 2 cut(s) 565, 1028
BseRI GAGGAG 2 cut(s) 938, 1193
BseSI GKGCMC 2 cut(s) 266, 1084
BseXI GCAGC 1 cut(s) 259
BseYI CCCAGC 1 cut(s) 297
BsgI GTGCAG 1 cut(s) 470
BshFI GGCC 4 cut(s) 49, 96, 314, 1000
BsiSI CCGG 4 cut(s) 51, 83, 93, 740
BslI CCNNNNNNNGG 1 cut(s) 596
BsmAI GTCTC 2 cut(s) 618, 788
BsnI GGCC 4 cut(s) 49, 96, 314, 1000
Bso31I GGTCTC 1 cut(s) 788
Bsp1286I GDGCHC 4 cut(s) 138, 266, 740, 1084
Bsp143I GATC 8 cut(s) 391, 586, 759, 772, 916, 1098, 1131, 1144
Bsp19I CCATGG 1 cut(s) 595
BspACI CCGC 2 cut(s) 221, 499
BspANI GGCC 4 cut(s) 49, 96, 314, 1000
BspCNI CTCAG 2 cut(s) 571, 1148
BspLI GGNNCC 3 cut(s) 201, 315, 918
BspMAI CTGCAG 2 cut(s) 116, 322
BspPI GGATC 5 cut(s) 581, 754, 911, 924, 1139
BspQI GCTCTTC 1 cut(s) 624
BspTNI GGTCTC 1 cut(s) 788
BsrBI CCGCTC 1 cut(s) 499
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 565, 1028
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 3 cut(s) 97, 595, 994
BssMI GATC 8 cut(s) 391, 586, 759, 772, 916, 1098, 1131, 1144
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 4 cut(s) 73, 353, 522, 767
Bst6I CTCTTC 2 cut(s) 610, 624
BstACI GRCGYC 1 cut(s) 21
BstC8I GCNNGC 3 cut(s) 56, 402, 528
BstDEI CTNAG 4 cut(s) 579, 680, 1135, 1215
BstDSI CCRYGG 1 cut(s) 595
BstF5I GGATG 2 cut(s) 120, 1262
BstKTI GATC 8 cut(s) 394, 589, 762, 775, 919, 1101, 1134, 1147
BstMAI GTCTC 2 cut(s) 618, 788
BstMBI GATC 8 cut(s) 391, 586, 759, 772, 916, 1098, 1131, 1144
BstMWI GCNNNNNNNGC 5 cut(s) 55, 60, 102, 111, 1088
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 4 cut(s) 49, 81, 96, 738
BstSFI CTRYAG 3 cut(s) 112, 318, 618
BstSLI GKGCMC 2 cut(s) 266, 1084
BstV1I GCAGC 1 cut(s) 259
BstX2I RGATCY 4 cut(s) 759, 916, 1098, 1131
BstYI RGATCY 4 cut(s) 759, 916, 1098, 1131
BsuRI GGCC 4 cut(s) 49, 96, 314, 1000
BtgI CCRYGG 1 cut(s) 595
BtsCI GGATG 2 cut(s) 120, 1262
BtsI GCAGTG 1 cut(s) 971
BtsIMutI CAGTG 5 cut(s) 375, 763, 971, 1091, 1247
Cac8I GCNNGC 3 cut(s) 56, 402, 528
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 4 cut(s) 48, 313, 575, 998
CseI GACGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 549
CviAII CATG 5 cut(s) 270, 306, 368, 475, 596
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 4 cut(s) 579, 680, 1135, 1215
DpnI GATC 8 cut(s) 393, 588, 761, 774, 918, 1100, 1133, 1146
DpnII GATC 8 cut(s) 391, 586, 759, 772, 916, 1098, 1131, 1144
DraI TTTAAA 1 cut(s) 951
DraIII CACNNNGTG 1 cut(s) 977
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 610, 624
EarI CTCTTC 2 cut(s) 610, 624
Eco130I CCWWGG 1 cut(s) 595
Eco24I GRGCYC 2 cut(s) 138, 740
Eco31I GGTCTC 1 cut(s) 788
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 575
Eco57I CTGAAG 2 cut(s) 740, 1115
EcoO109I RGGNCCY 2 cut(s) 575, 998
EcoRI GAATTC 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 96
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 595
EcoT38I GRGCYC 2 cut(s) 138, 740
ErhI CCWWGG 1 cut(s) 595
FaeI CATG 5 cut(s) 273, 309, 371, 478, 599
FalI AAGNNNNNCTT 2 cut(s) 770, 802
FatI CATG 5 cut(s) 269, 305, 367, 474, 595
FbaI TGATCA 1 cut(s) 772
Fnu4HI GCNGC 2 cut(s) 248, 500
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 2 cut(s) 138, 740
Fsp4HI GCNGC 2 cut(s) 248, 500
FspBI CTAG 2 cut(s) 552, 1058
GluI GCNGC 2 cut(s) 248, 500
GsaI CCCAGC 1 cut(s) 301
GsuI CTGGAG 2 cut(s) 548, 834
HaeIII GGCC 4 cut(s) 49, 96, 314, 1000
HapII CCGG 4 cut(s) 51, 83, 93, 740
HgaI GACGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 5 cut(s) 273, 309, 371, 478, 599
HinfI GANTC 5 cut(s) 117, 406, 542, 910, 1219
HpaII CCGG 4 cut(s) 51, 83, 93, 740
HphI GGTGA 5 cut(s) 61, 235, 427, 551, 959
Hpy166II GTNNAC 1 cut(s) 549
Hpy188I TCNGA 5 cut(s) 12, 349, 391, 759, 1224
Hpy8I GTNNAC 1 cut(s) 549
Hpy99I CGWCG 4 cut(s) 20, 23, 26, 29
HpyAV CCTTC 1 cut(s) 1119
HpyCH4III ACNGT 4 cut(s) 73, 353, 522, 767
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 7 cut(s) 114, 320, 367, 487, 526, 989, 1104
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 60, 102, 111, 1088
HpyF3I CTNAG 4 cut(s) 579, 680, 1135, 1215
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 5 cut(s) 273, 309, 371, 478, 599
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 8 cut(s) 391, 586, 759, 772, 916, 1098, 1131, 1144
LguI GCTCTTC 1 cut(s) 624
LmnI GCTCC 4 cut(s) 255, 478, 496, 925
Lsp1109I GCAGC 1 cut(s) 259
LweI GCATC 2 cut(s) 609, 951
MaeI CTAG 2 cut(s) 552, 1058
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 4 cut(s) 373, 421, 715, 1072
MalI GATC 8 cut(s) 393, 588, 761, 774, 918, 1100, 1133, 1146
MbiI CCGCTC 1 cut(s) 499
MboI GATC 8 cut(s) 391, 586, 759, 772, 916, 1098, 1131, 1144
MboII GAAGA 4 cut(s) 451, 597, 641, 1108
MflI RGATCY 4 cut(s) 759, 916, 1098, 1131
MhlI GDGCHC 4 cut(s) 138, 266, 740, 1084
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 1 cut(s) 1155
MnlI CCTC 6 cut(s) 134, 916, 989, 1122, 1150, 1171
MseI TTAA 3 cut(s) 75, 332, 950
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 4 cut(s) 51, 83, 93, 740
MspR9I CCNGG 4 cut(s) 51, 83, 98, 740
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 5 cut(s) 55, 60, 102, 111, 1088
NciI CCSGG 3 cut(s) 51, 83, 740
NcoI CCATGG 1 cut(s) 595
NdeII GATC 8 cut(s) 391, 586, 759, 772, 916, 1098, 1131, 1144
NlaIII CATG 5 cut(s) 273, 309, 371, 478, 599
NlaIV GGNNCC 3 cut(s) 201, 315, 918
NmeAIII GCCGAG 1 cut(s) 1091
NmuCI GTSAC 3 cut(s) 373, 421, 715
NspI RCATGY 1 cut(s) 309
PciI ACATGT 1 cut(s) 305
PciSI GCTCTTC 1 cut(s) 624
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 4 cut(s) 117, 406, 910, 1219
PflFI GACNNNGTC 3 cut(s) 20, 23, 443
PkrI GCNGC 2 cut(s) 249, 501
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
PpuMI RGGWCCY 1 cut(s) 575
PscI ACATGT 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 96
PspFI CCCAGC 1 cut(s) 297
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 3 cut(s) 201, 315, 918
PspPI GGNCC 4 cut(s) 48, 313, 575, 998
PspPPI RGGWCCY 1 cut(s) 575
PstI CTGCAG 2 cut(s) 116, 322
PsuI RGATCY 4 cut(s) 759, 916, 1098, 1131
PsyI GACNNNGTC 3 cut(s) 20, 23, 443
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 624
SaqAI TTAA 3 cut(s) 75, 332, 950
SatI GCNGC 2 cut(s) 248, 500
Sau3AI GATC 8 cut(s) 391, 586, 759, 772, 916, 1098, 1131, 1144
Sau96I GGNCC 4 cut(s) 48, 313, 575, 998
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 4 cut(s) 51, 83, 98, 740
SduI GDGCHC 4 cut(s) 138, 266, 740, 1084
SfaNI GCATC 2 cut(s) 609, 951
SfcI CTRYAG 3 cut(s) 112, 318, 618
SinI GGWCC 1 cut(s) 575
SmlI CTYRAG 2 cut(s) 395, 672
SmoI CTYRAG 2 cut(s) 395, 672
SpeI ACTAGT 2 cut(s) 551, 1057
SsiI CCGC 2 cut(s) 221, 499
SspMI CTAG 2 cut(s) 552, 1058
StyD4I CCNGG 4 cut(s) 49, 81, 96, 738
StyI CCWWGG 1 cut(s) 595
TaaI ACNGT 4 cut(s) 73, 353, 522, 767
TaiI ACGT 1 cut(s) 24
TaqI TCGA 4 cut(s) 126, 161, 233, 841
TatI WGTACW 1 cut(s) 548
TauI GCSGC 1 cut(s) 502
TfiI GAWTC 4 cut(s) 117, 406, 910, 1219
Tru1I TTAA 3 cut(s) 75, 332, 950
Tru9I TTAA 3 cut(s) 75, 332, 950
TscAI CASTG 5 cut(s) 382, 770, 978, 1098, 1254
TseFI GTSAC 3 cut(s) 373, 421, 715
TseI GCWGC 1 cut(s) 247
Tsp45I GTSAC 3 cut(s) 373, 421, 715
TspDTI ATGAA 1 cut(s) 451
TspGWI ACGGA 3 cut(s) 247, 270, 656
TspRI CASTG 5 cut(s) 382, 770, 978, 1098, 1254
Tth111I GACNNNGTC 3 cut(s) 20, 23, 443
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 6 cut(s) 6, 205, 235, 381, 946, 1190
XceI RCATGY 1 cut(s) 309
XspI CTAG 2 cut(s) 552, 1058
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.