RchiOBHm_Chr5g0063651

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003332
Physical Location & Seq
Reverse (-)
69315388 .. 69319510
4123 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33972

Sequence Viewer

Length: 1263 bp
ATGGGAATCCCGAGCGACGACGTCGTTGTATTCCAGAAAGCTAAACGTCCTGGCGAGGCTTCCGTCATCACCGTTAATTGTCCGGATAAGACCGGCCTGGGATGTGATATTTGCAGGATCATCCTCGATTTTGGGCTCTGCATCAGAAAAGCTGATTTTTCAACTGATGGGATATGGTGCTACGTTGTATTATGGGTGGTTCCTCATTCTAACTCAACAACAGTGAAATGGTCGAATTTGGAGAACCAGCTCCTATCCGTGTGCCCGTCATGTTCTGTTCCGTATCACTTCTACCCCCAGACTACATGTTCCTCGCCCCCTGCAGTTTATCTATTGAAGTTTCTTTGCTCTGACCGTAATGGATTATTGCATGATGTCACTGAAATTCTGTCTGATCTTGAGCTTTCGATTCAAAGGGTGAAAGTGACAACAACACCAGATGACAGGGTCTTGGACCTGTTCTTCATAACAGATAACATGGATCTTCTGCACACAAAGGAGCGGCAAGATAAGACATTGAAACAGTTGCAAGCCGTATTGGGTGAGTCGTGTACCAGTTGCGAACTTCAGTTGGTAGGTCCCGAGTATGATCCCCACCATGGCATTCCTTCTCTGTCTCCTGTAGTAGCTGAAGAGCTATTTAGGTGTGAGCTGTTGGATAAAGAAATCCGTTCTCAAGCACTTAGTCCTGATATGACGAAATTGAAGAACGCTAATGTAACAATAGACAACTCATTGAGCCCAGCACATACATTACTTCAGATCCACTGTGCTGATCACAAGGGTCTCTTGTATGACATTATGAGAACTTTGAAAGACAGCAACATCAAGATAGCTTATGGTCGATTTTCTCCAACTACAAAAGGGTATCGGGATTTAGACCTTTTTATTCAGCAAAAGGATGGGAAAAAGATTCAGGATCCGGAGGCGCAGAGTGCATTGTGTTTGAGTTTGAAGGTGGAGATGCTTCACCCACTGCGTGTTATCATTGCAAACCGAGGGCCTGATACAGAATTGTTGGTTGCTAATCCAGTTGAACTATCTGGAAAGGGGCGACCACTAGTTTTCTATGATGTTACTTTGGCTCTAAAAGCACTGAGGATCTGCATTTTCTCGGCTGAAATTGGAAGGATCTCAGCATCAGGTCGTGAATGGGAGGTGTATAGTTTTCTGTTGGAGGAGAATTCCAAATTTCAATTATCAAACATGGTTGCTAAGAATCAGATTGTAGATAGAGTTAGAAGAACACTGATGGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

420

Amino Acids

47.11

Weight (kDa)

6.09

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACR10_N PF24914 12 - 89 7.5e-40 ACT domain-containing protein ACR10, N-terminal
ACT PF01842 111 - 171 4.4e-09 ACT domain
ACT_ACR9_3rd PF24931 239 - 336 5.9e-48 ACR9-like, ACT-like domain
ACT_ACR9_C PF24926 337 - 419 6.1e-38 ACT domain-containing protein ACR9, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccBSI CCGCTC 1 cut(s) 502
AccIII TCCGGA 2 cut(s) 82, 922
AciI CCGC 1 cut(s) 502
AclWI GGATC 8 cut(s) 125, 489, 584, 757, 914, 927, 1109, 1139
AcsI RAATTY 4 cut(s) 235, 384, 1183, 1190
AcuI CTGAAG 3 cut(s) 551, 651, 743
AcyI GRCGYC 1 cut(s) 21
AdeI CACNNNGTG 1 cut(s) 980
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 9 cut(s) 162, 337, 413, 520, 706, 814, 955, 1037, 1196
AhlI ACTAGT 1 cut(s) 1060
AjnI CCWGG 2 cut(s) 49, 96
AluBI AGCT 8 cut(s) 41, 152, 250, 403, 629, 637, 652, 836
AluI AGCT 8 cut(s) 41, 152, 250, 403, 629, 637, 652, 836
Alw26I GTCTC 2 cut(s) 621, 791
AlwI GGATC 8 cut(s) 125, 489, 584, 757, 914, 927, 1109, 1139
Ama87I CYCGRG 2 cut(s) 10, 581
Aor13HI TCCGGA 2 cut(s) 82, 922
AoxI GGCC 2 cut(s) 94, 1001
ApoI RAATTY 4 cut(s) 235, 384, 1183, 1190
AspLEI GCGC 1 cut(s) 931
AspS9I GGNCC 3 cut(s) 454, 578, 1001
AsuHPI GGTGA 4 cut(s) 61, 430, 554, 962
AvaI CYCGRG 2 cut(s) 10, 581
AvaII GGWCC 2 cut(s) 454, 578
BaeGI GKGCMC 1 cut(s) 266
BamHI GGATCC 1 cut(s) 919
BanII GRGCYC 2 cut(s) 138, 743
BccI CCATC 4 cut(s) 161, 896, 1246, 1251
BceAI ACGGC 1 cut(s) 518
BciT130I CCWGG 2 cut(s) 51, 98
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 2 cut(s) 621, 791
BcuI ACTAGT 1 cut(s) 1060
BfaI CTAG 1 cut(s) 1061
BfmI CTRYAG 2 cut(s) 321, 621
BisI GCNGC 1 cut(s) 503
BlsI GCNGC 1 cut(s) 504
Bme1390I CCNGG 2 cut(s) 51, 98
Bme18I GGWCC 2 cut(s) 454, 578
BmeT110I CYCGRG 2 cut(s) 10, 581
BmgT120I GGNCC 3 cut(s) 454, 578, 1001
BmiI GGNNCC 3 cut(s) 201, 580, 921
BmrFI CCNGG 2 cut(s) 51, 98
BmsI GCATC 3 cut(s) 150, 954, 1148
BpuEI CTTGAG 2 cut(s) 419, 660
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 791
BsaJI CCNNGG 3 cut(s) 97, 598, 997
BsaWI WCCGGW 2 cut(s) 82, 922
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 555, 1031
Bse3DI GCAATG 1 cut(s) 987
BseAI TCCGGA 2 cut(s) 82, 922
BseBI CCWGG 2 cut(s) 51, 98
BseDI CCNNGG 3 cut(s) 97, 598, 997
BseGI GGATG 4 cut(s) 107, 120, 907, 1262
BseLI CCNNNNNNNGG 1 cut(s) 599
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 2 cut(s) 1088, 1149
BseNI ACTGG 2 cut(s) 555, 1031
BseRI GAGGAG 1 cut(s) 1193
BseSI GKGCMC 1 cut(s) 266
BseYI CCCAGC 1 cut(s) 742
BsgI GTGCAG 1 cut(s) 473
BshFI GGCC 2 cut(s) 96, 1003
BsiHKCI CYCGRG 2 cut(s) 10, 581
BsiSI CCGG 3 cut(s) 83, 93, 923
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 2 cut(s) 621, 791
BsmFI GGGAC 1 cut(s) 564
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 2 cut(s) 96, 1003
Bso31I GGTCTC 1 cut(s) 791
BsoBI CYCGRG 2 cut(s) 10, 581
Bsp1286I GDGCHC 3 cut(s) 138, 266, 743
Bsp13I TCCGGA 2 cut(s) 82, 922
Bsp143I GATC 9 cut(s) 117, 394, 481, 589, 762, 775, 919, 1101, 1131
Bsp19I CCATGG 1 cut(s) 598
BspACI CCGC 1 cut(s) 502
BspANI GGCC 2 cut(s) 96, 1003
BspCNI CTCAG 2 cut(s) 1089, 1148
BspEI TCCGGA 2 cut(s) 82, 922
BspLI GGNNCC 3 cut(s) 201, 580, 921
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 8 cut(s) 125, 489, 584, 757, 914, 927, 1109, 1139
BspQI GCTCTTC 1 cut(s) 627
BspTNI GGTCTC 1 cut(s) 791
BsrBI CCGCTC 1 cut(s) 502
BsrDI GCAATG 1 cut(s) 987
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 555, 1031
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 3 cut(s) 97, 598, 997
BssMI GATC 9 cut(s) 117, 394, 481, 589, 762, 775, 919, 1101, 1131
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 598
Bst2UI CCWGG 2 cut(s) 51, 98
Bst4CI ACNGT 5 cut(s) 73, 223, 356, 525, 770
Bst6I CTCTTC 1 cut(s) 627
BstACI GRCGYC 1 cut(s) 21
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 4 cut(s) 683, 1097, 1135, 1215
BstDSI CCRYGG 1 cut(s) 598
BstF5I GGATG 4 cut(s) 107, 120, 907, 1262
BstHHI GCGC 1 cut(s) 931
BstKTI GATC 9 cut(s) 120, 397, 484, 592, 765, 778, 922, 1104, 1134
BstMAI GTCTC 2 cut(s) 621, 791
BstMBI GATC 9 cut(s) 117, 394, 481, 589, 762, 775, 919, 1101, 1131
BstMWI GCNNNNNNNGC 2 cut(s) 935, 1091
BstNI CCWGG 2 cut(s) 51, 98
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 2 cut(s) 49, 96
BstSFI CTRYAG 2 cut(s) 321, 621
BstSLI GKGCMC 1 cut(s) 266
BstX2I RGATCY 5 cut(s) 481, 762, 919, 1101, 1131
BstYI RGATCY 5 cut(s) 481, 762, 919, 1101, 1131
BsuRI GGCC 2 cut(s) 96, 1003
BtgI CCRYGG 1 cut(s) 598
BtsCI GGATG 4 cut(s) 107, 120, 907, 1262
BtsI GCAGTG 1 cut(s) 974
BtsIMutI CAGTG 6 cut(s) 228, 378, 766, 974, 1094, 1247
Cac8I GCNNGC 1 cut(s) 531
CfoI GCGC 1 cut(s) 931
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 3 cut(s) 454, 578, 1001
Csp6I GTAC 1 cut(s) 552
CviAII CATG 6 cut(s) 270, 306, 371, 478, 599, 1207
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 4 cut(s) 683, 1097, 1135, 1215
DpnI GATC 9 cut(s) 119, 396, 483, 591, 764, 777, 921, 1103, 1133
DpnII GATC 9 cut(s) 117, 394, 481, 589, 762, 775, 919, 1101, 1131
DraIII CACNNNGTG 1 cut(s) 980
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Eco130I CCWWGG 1 cut(s) 598
Eco24I GRGCYC 2 cut(s) 138, 743
Eco31I GGTCTC 1 cut(s) 791
Eco47I GGWCC 2 cut(s) 454, 578
Eco57I CTGAAG 3 cut(s) 551, 651, 743
Eco88I CYCGRG 2 cut(s) 10, 581
EcoO109I RGGNCCY 2 cut(s) 578, 1001
EcoRI GAATTC 1 cut(s) 1183
EcoRII CCWGG 2 cut(s) 49, 96
EcoT14I CCWWGG 1 cut(s) 598
EcoT38I GRGCYC 2 cut(s) 138, 743
ErhI CCWWGG 1 cut(s) 598
FaeI CATG 6 cut(s) 273, 309, 374, 481, 602, 1210
FalI AAGNNNNNCTT 2 cut(s) 773, 805
FaqI GGGAC 1 cut(s) 564
FatI CATG 6 cut(s) 269, 305, 370, 477, 598, 1206
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 1 cut(s) 503
FokI GGATG 3 cut(s) 107, 114, 914
FriOI GRGCYC 2 cut(s) 138, 743
Fsp4HI GCNGC 1 cut(s) 503
FspBI CTAG 1 cut(s) 1061
GlaI GCGC 1 cut(s) 930
GluI GCNGC 1 cut(s) 503
GsaI CCCAGC 1 cut(s) 746
HaeIII GGCC 2 cut(s) 96, 1003
HapII CCGG 3 cut(s) 83, 93, 923
HhaI GCGC 1 cut(s) 931
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 6 cut(s) 273, 309, 374, 481, 602, 1210
Hin6I GCGC 1 cut(s) 929
HinP1I GCGC 1 cut(s) 929
HinfI GANTC 5 cut(s) 6, 409, 545, 913, 1219
HpaII CCGG 3 cut(s) 83, 93, 923
HphI GGTGA 4 cut(s) 61, 430, 554, 962
Hpy166II GTNNAC 1 cut(s) 552
Hpy188I TCNGA 5 cut(s) 146, 352, 394, 762, 1224
Hpy8I GTNNAC 1 cut(s) 552
Hpy99I CGWCG 3 cut(s) 20, 23, 26
HpyAV CCTTC 3 cut(s) 618, 949, 1122
HpyCH4III ACNGT 5 cut(s) 73, 223, 356, 525, 770
HpyCH4IV ACGT 3 cut(s) 21, 46, 183
HpyCH4V TGCA 9 cut(s) 114, 141, 323, 370, 490, 529, 938, 992, 1107
HpyF10VI GCNNNNNNNGC 2 cut(s) 935, 1091
HpyF3I CTNAG 4 cut(s) 683, 1097, 1135, 1215
HpySE526I ACGT 3 cut(s) 21, 46, 183
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 6 cut(s) 273, 309, 374, 481, 602, 1210
HspAI GCGC 1 cut(s) 929
Kpn2I TCCGGA 2 cut(s) 82, 922
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 9 cut(s) 117, 394, 481, 589, 762, 775, 919, 1101, 1131
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 2 cut(s) 255, 499
LweI GCATC 3 cut(s) 150, 954, 1148
MaeI CTAG 1 cut(s) 1061
MaeII ACGT 3 cut(s) 21, 46, 183
MaeIII GTNAC 4 cut(s) 376, 424, 718, 1075
MalI GATC 9 cut(s) 119, 396, 483, 591, 764, 777, 921, 1103, 1133
MbiI CCGCTC 1 cut(s) 502
MboI GATC 9 cut(s) 117, 394, 481, 589, 762, 775, 919, 1101, 1131
MboII GAAGA 5 cut(s) 454, 476, 644, 718, 1254
MflI RGATCY 5 cut(s) 481, 762, 919, 1101, 1131
MhlI GDGCHC 3 cut(s) 138, 266, 743
MluCI AATT 9 cut(s) 76, 235, 384, 701, 1013, 1122, 1183, 1190, 1196
MlyI GAGTC 1 cut(s) 554
MmeI TCCRAC 3 cut(s) 636, 878, 1155
MnlI CCTC 9 cut(s) 49, 134, 213, 322, 919, 992, 1092, 1150, 1171
MroI TCCGGA 2 cut(s) 82, 922
MseI TTAA 1 cut(s) 75
MspI CCGG 3 cut(s) 83, 93, 923
MspR9I CCNGG 2 cut(s) 51, 98
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 2 cut(s) 51, 98
MwoI GCNNNNNNNGC 2 cut(s) 935, 1091
NcoI CCATGG 1 cut(s) 598
NdeII GATC 9 cut(s) 117, 394, 481, 589, 762, 775, 919, 1101, 1131
NlaIII CATG 6 cut(s) 273, 309, 374, 481, 602, 1210
NlaIV GGNNCC 3 cut(s) 201, 580, 921
NmeAIII GCCGAG 1 cut(s) 1094
NmuCI GTSAC 2 cut(s) 376, 424
NspI RCATGY 1 cut(s) 309
PciI ACATGT 1 cut(s) 305
PciSI GCTCTTC 1 cut(s) 627
PctI GAATGC 1 cut(s) 603
PfeI GAWTC 4 cut(s) 6, 409, 913, 1219
PflFI GACNNNGTC 2 cut(s) 20, 446
PkrI GCNGC 1 cut(s) 504
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
PpuMI RGGWCCY 1 cut(s) 578
PscI ACATGT 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 578
Psp6I CCWGG 2 cut(s) 49, 96
PspFI CCCAGC 1 cut(s) 742
PspGI CCWGG 2 cut(s) 49, 96
PspN4I GGNNCC 3 cut(s) 201, 580, 921
PspPI GGNCC 3 cut(s) 454, 578, 1001
PspPPI RGGWCCY 1 cut(s) 578
PstI CTGCAG 1 cut(s) 325
PsuI RGATCY 5 cut(s) 481, 762, 919, 1101, 1131
PsyI GACNNNGTC 2 cut(s) 20, 446
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 1 cut(s) 503
Sau3AI GATC 9 cut(s) 117, 394, 481, 589, 762, 775, 919, 1101, 1131
Sau96I GGNCC 3 cut(s) 454, 578, 1001
SchI GAGTC 1 cut(s) 554
ScrFI CCNGG 2 cut(s) 51, 98
SduI GDGCHC 3 cut(s) 138, 266, 743
SfaNI GCATC 3 cut(s) 150, 954, 1148
SfcI CTRYAG 2 cut(s) 321, 621
SinI GGWCC 2 cut(s) 454, 578
SmlI CTYRAG 2 cut(s) 398, 675
SmoI CTYRAG 2 cut(s) 398, 675
SpeI ACTAGT 1 cut(s) 1060
Sse9I AATT 9 cut(s) 76, 235, 384, 701, 1013, 1122, 1183, 1190, 1196
SsiI CCGC 1 cut(s) 502
SspMI CTAG 1 cut(s) 1061
StyD4I CCNGG 2 cut(s) 49, 96
StyI CCWWGG 1 cut(s) 598
TaaI ACNGT 5 cut(s) 73, 223, 356, 525, 770
TaiI ACGT 3 cut(s) 24, 49, 186
TaqI TCGA 4 cut(s) 126, 233, 407, 844
TasI AATT 9 cut(s) 76, 235, 384, 701, 1013, 1122, 1183, 1190, 1196
TauI GCSGC 1 cut(s) 505
TfiI GAWTC 4 cut(s) 6, 409, 913, 1219
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 6 cut(s) 228, 385, 773, 981, 1101, 1254
TseFI GTSAC 2 cut(s) 376, 424
Tsp45I GTSAC 2 cut(s) 376, 424
TspDTI ATGAA 1 cut(s) 454
TspGWI ACGGA 4 cut(s) 52, 247, 270, 659
TspRI CASTG 6 cut(s) 228, 385, 773, 981, 1101, 1254
Tth111I GACNNNGTC 2 cut(s) 20, 446
VpaK11BI GGWCC 2 cut(s) 454, 578
XapI RAATTY 4 cut(s) 235, 384, 1183, 1190
XceI RCATGY 1 cut(s) 309
XspI CTAG 1 cut(s) 1061
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.