MD11G1143600.v1.1

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003332
Physical Location & Seq
Forward (+)
13362328 .. 13365648
3321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1143600.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGGGTGTGCCGAGCGACGACGTCGTTGTGATCCAGAAAGGAAGGCGGCCCGGCGAGCCCTGCATCATCACCGTCAATTGCCCGGACAAGACCGGCCTCGGGTGCGATATTTGCAGAATCATCCTCGATTTTGGCCTTCTAATAGAAAAAGCAGATTTTTCGACTGATGGGATATGGTGCTACATAGTGTTATGGGTGTTTCCGGATTCCAGCTCCCCAGTTGTGAAATTGTCAAATTTGAAGAACCAGCTACAATCTGTATGCCCATCATGTTCAGTGCCGTTTTACTTGTACCAGCAGGCCACAAGATCGTCGTCCTCACCAGTTTATCTGTTGACGTTTCTTTGCCTCGACCGCAAGGGATTGTTGCATGATGTTACAAAAACTCTCTCAGAGCTTGAGCTTTCGATTCAGCGAGTGAAAGTTACGACAACCCCAGATGATAGGGTCTTGGACCTCTTCTTTATAACAGATAACACGGACCTTTTACACACAAAGGAACGCCAAGATGAGACAATAAAACAATTGCGTGATGTTTTGGGTGAGTCATGTATCAGCTGCGAACTTCAGCTGGTAGGTCCCGAATATGAATCCCTCCATGGAATCCCTTCCCTTTCTCCTGTGGTGGCTGAAGAGCTATTTAGATGTGAGTTATTGGATAAAGAAACCCGGTCTCAAGCTCTTAGCCCAGATATGACAAAGTTGAAGAAAACCAATGTGACCATGGACAATTCTTTGAGCCCTGCTCATACATTACTTCAAATCCACTGTGCTGACCACAAAGGTCTCTTGTATGACATTCTGAGAACTTTGAAAGACTGCAATATCAAGATAGCTTATGGTAGAATTTCTCCAAATATTAAAGGCTATCGCGATTTAGACCTATTCATTCAGCAAAATGATGGGAAAAAGTTTGTCGATCCCGATAAGCAGAGTGCACTGTATTTGCGGTTGAAGGTGGAGATGCTTCACCCATTACGTGTTATCATTGCAAACCGAGGGCCAGATACTGAACTGTTAGTTGCTAATCCAGTTGAGCTATCTGGAAAGGGAAGACCACGAGTTTTCTACGATGTTACAACTGCTCTAAAAACACTTGGGATATGCATTTTCTCTGCCGAAATCGGGAGGGTAGCGGCATCAGATCGTGAATGGGAGGTGTATACCTTTTTGCTGGAAGAGAACCACAACTTTCAGTTATCAAATATGGTAGCTAAGAATCAGATTGCAGATAGAGTTAGAAGAACCTTGATGGGCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

421

Amino Acids

47.36

Weight (kDa)

5.97

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACR10_N PF24914 11 - 89 5.6e-38 ACT domain-containing protein ACR10, N-terminal
ACT_ACR9_3rd PF24931 110 - 179 8.9e-06 ACR9-like, ACT-like domain
ACT PF01842 111 - 169 3.1e-09 ACT domain
ACT_ACR9_3rd PF24931 239 - 336 2.2e-47 ACR9-like, ACT-like domain
ACT_ACR9_C PF24926 337 - 419 7.5e-39 ACT domain-containing protein ACR9, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AatII GACGTC 1 cut(s) 24
AccI GTMKAC 1 cut(s) 1163
AccII CGCG 1 cut(s) 873
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 4 cut(s) 46, 355, 949, 1136
AclWI GGATC 2 cut(s) 25, 914
AcsI RAATTY 2 cut(s) 235, 846
AcuI CTGAAG 2 cut(s) 551, 651
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 2 cut(s) 99, 1125
AflIII ACRYGT 1 cut(s) 979
AgsI TTSAA 5 cut(s) 241, 706, 761, 814, 955
Alw21I GWGCWC 1 cut(s) 940
Alw26I GTCTC 3 cut(s) 506, 678, 791
Alw44I GTGCAC 1 cut(s) 936
AlwI GGATC 2 cut(s) 25, 914
AlwNI CAGNNNCTG 1 cut(s) 1010
Ama87I CYCGRG 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 5 cut(s) 47, 94, 133, 300, 1001
ApaLI GTGCAC 1 cut(s) 936
ApeKI GCWGC 1 cut(s) 558
ApoI RAATTY 2 cut(s) 235, 846
Asp700I GAANNNNTTC 1 cut(s) 607
AspS9I GGNCC 5 cut(s) 48, 454, 481, 578, 1001
AsuC2I CCSGG 3 cut(s) 51, 83, 670
AsuHPI GGTGA 4 cut(s) 61, 312, 554, 962
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 3 cut(s) 454, 481, 578
BaeGI GKGCMC 1 cut(s) 940
BanII GRGCYC 2 cut(s) 60, 743
BauI CACGAG 1 cut(s) 1059
BbsI GAAGAC 1 cut(s) 1060
Bbv12I GWGCWC 1 cut(s) 940
BbvI GCAGC 1 cut(s) 545
BccI CCATC 4 cut(s) 161, 274, 896, 1246
BceAI ACGGC 1 cut(s) 265
BcgI CGANNNNNNTGC 2 cut(s) 141, 175
BcnI CCSGG 3 cut(s) 51, 83, 670
BcoDI GTCTC 3 cut(s) 506, 678, 791
BisI GCNGC 3 cut(s) 47, 559, 1137
BlsI GCNGC 3 cut(s) 48, 560, 1138
Bme1390I CCNGG 3 cut(s) 51, 83, 670
Bme18I GGWCC 3 cut(s) 454, 481, 578
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 5 cut(s) 48, 454, 481, 578, 1001
BmiI GGNNCC 1 cut(s) 580
BmrFI CCNGG 3 cut(s) 51, 83, 670
BmrI ACTGGG 1 cut(s) 212
BmsI GCATC 3 cut(s) 72, 954, 1148
BmuI ACTGGG 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 1060
BplI GAGNNNNNCTC 2 cut(s) 730, 762
BpuEI CTTGAG 2 cut(s) 419, 660
BpuMI CCSGG 3 cut(s) 51, 83, 670
BsaAI YACGTR 1 cut(s) 980
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 2 cut(s) 678, 791
BsaJI CCNNGG 4 cut(s) 97, 598, 723, 997
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 1125
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 3 cut(s) 218, 323, 1031
Bse3DI GCAATG 1 cut(s) 987
BseAI TCCGGA 1 cut(s) 202
BseDI CCNNGG 4 cut(s) 97, 598, 723, 997
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 2 cut(s) 99, 1125
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 2 cut(s) 405, 794
BseNI ACTGG 3 cut(s) 218, 323, 1031
BseSI GKGCMC 1 cut(s) 940
BseXI GCAGC 1 cut(s) 545
Bsh1236I CGCG 1 cut(s) 873
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 5 cut(s) 49, 96, 135, 302, 1003
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 940
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 5 cut(s) 51, 83, 93, 203, 670
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 2 cut(s) 99, 1125
BsmAI GTCTC 3 cut(s) 506, 678, 791
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 5 cut(s) 49, 96, 135, 302, 1003
Bso31I GGTCTC 2 cut(s) 678, 791
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 3 cut(s) 60, 743, 940
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 4 cut(s) 30, 308, 919, 1144
Bsp19I CCATGG 2 cut(s) 598, 723
Bsp68I TCGCGA 1 cut(s) 873
BspACI CCGC 4 cut(s) 46, 355, 949, 1136
BspANI GGCC 5 cut(s) 49, 96, 135, 302, 1003
BspCNI CTCAG 2 cut(s) 404, 795
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 1 cut(s) 873
BspLI GGNNCC 1 cut(s) 580
BspPI GGATC 2 cut(s) 25, 914
BspQI GCTCTTC 1 cut(s) 627
BspTNI GGTCTC 2 cut(s) 678, 791
BsrDI GCAATG 1 cut(s) 987
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 3 cut(s) 218, 323, 1031
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 4 cut(s) 97, 598, 723, 997
BssMI GATC 4 cut(s) 30, 308, 919, 1144
BssNAI GTATAC 1 cut(s) 1164
BssNI GRCGYC 1 cut(s) 21
BssSI CACGAG 1 cut(s) 1059
BssT1I CCWWGG 2 cut(s) 598, 723
Bst1107I GTATAC 1 cut(s) 1164
Bst2BI CACGAG 1 cut(s) 1059
Bst4CI ACNGT 4 cut(s) 73, 770, 942, 1017
Bst6I CTCTTC 3 cut(s) 464, 627, 1173
BstACI GRCGYC 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 980
BstC8I GCNNGC 2 cut(s) 56, 300
BstDEI CTNAG 4 cut(s) 391, 683, 803, 1215
BstDSI CCRYGG 2 cut(s) 598, 723
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 1 cut(s) 873
BstKTI GATC 4 cut(s) 33, 311, 922, 1147
BstMAI GTCTC 3 cut(s) 506, 678, 791
BstMBI GATC 4 cut(s) 30, 308, 919, 1144
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 5 cut(s) 55, 60, 102, 111, 354
BstSCI CCNGG 3 cut(s) 49, 81, 668
BstSLI GKGCMC 1 cut(s) 940
BstUI CGCG 1 cut(s) 873
BstV1I GCAGC 1 cut(s) 545
BstV2I GAAGAC 1 cut(s) 1060
BstZ17I GTATAC 1 cut(s) 1164
BsuRI GGCC 5 cut(s) 49, 96, 135, 302, 1003
BtgI CCRYGG 2 cut(s) 598, 723
BtsCI GGATG 1 cut(s) 120
BtsIMutI CAGTG 3 cut(s) 282, 766, 938
BtuMI TCGCGA 1 cut(s) 873
Cac8I GCNNGC 2 cut(s) 56, 300
CaiI CAGNNNCTG 1 cut(s) 1010
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 5 cut(s) 48, 454, 481, 578, 1001
Csp6I GTAC 1 cut(s) 292
CviAII CATG 5 cut(s) 270, 371, 549, 599, 724
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 4 cut(s) 391, 683, 803, 1215
DpnI GATC 4 cut(s) 32, 310, 921, 1146
DpnII GATC 4 cut(s) 30, 308, 919, 1144
Eam1104I CTCTTC 3 cut(s) 464, 627, 1173
EarI CTCTTC 3 cut(s) 464, 627, 1173
Eco130I CCWWGG 2 cut(s) 598, 723
Eco24I GRGCYC 2 cut(s) 60, 743
Eco31I GGTCTC 2 cut(s) 678, 791
Eco47I GGWCC 3 cut(s) 454, 481, 578
Eco57I CTGAAG 2 cut(s) 551, 651
Eco88I CYCGRG 1 cut(s) 98
EcoO109I RGGNCCY 1 cut(s) 578
EcoT14I CCWWGG 2 cut(s) 598, 723
EcoT22I ATGCAT 1 cut(s) 1109
EcoT38I GRGCYC 2 cut(s) 60, 743
ErhI CCWWGG 2 cut(s) 598, 723
FaeI CATG 5 cut(s) 273, 374, 552, 602, 727
FaqI GGGAC 1 cut(s) 564
FatI CATG 5 cut(s) 269, 370, 548, 598, 723
FblI GTMKAC 1 cut(s) 1163
Fnu4HI GCNGC 3 cut(s) 47, 559, 1137
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 2 cut(s) 60, 743
Fsp4HI GCNGC 3 cut(s) 47, 559, 1137
GluI GCNGC 3 cut(s) 47, 559, 1137
HaeIII GGCC 5 cut(s) 49, 96, 135, 302, 1003
HapII CCGG 5 cut(s) 51, 83, 93, 203, 670
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 5 cut(s) 273, 374, 552, 602, 727
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HinfI GANTC 7 cut(s) 117, 206, 409, 545, 590, 603, 1219
HpaII CCGG 5 cut(s) 51, 83, 93, 203, 670
HphI GGTGA 4 cut(s) 61, 312, 554, 962
Hpy166II GTNNAC 3 cut(s) 336, 938, 1164
Hpy188I TCNGA 4 cut(s) 394, 804, 1144, 1224
Hpy188III TCNNGA 9 cut(s) 34, 203, 581, 829, 872, 923, 1044, 1126, 1148
Hpy8I GTNNAC 3 cut(s) 336, 938, 1164
Hpy99I CGWCG 4 cut(s) 20, 23, 26, 316
HpyAV CCTTC 4 cut(s) 36, 146, 618, 949
HpyCH4III ACNGT 4 cut(s) 73, 770, 942, 1017
HpyCH4IV ACGT 3 cut(s) 21, 338, 979
HpyCH4V TGCA 8 cut(s) 63, 114, 370, 822, 938, 992, 1107, 1229
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 60, 102, 111, 354
HpyF3I CTNAG 4 cut(s) 391, 683, 803, 1215
HpySE526I ACGT 3 cut(s) 21, 338, 979
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 5 cut(s) 273, 374, 552, 602, 727
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 4 cut(s) 30, 308, 919, 1144
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 1 cut(s) 218
Lsp1109I GCAGC 1 cut(s) 545
LweI GCATC 3 cut(s) 72, 954, 1148
MaeII ACGT 3 cut(s) 21, 338, 979
MaeIII GTNAC 4 cut(s) 376, 424, 718, 1075
MalI GATC 4 cut(s) 32, 310, 921, 1146
MboI GATC 4 cut(s) 30, 308, 919, 1144
MboII GAAGA 7 cut(s) 253, 451, 644, 718, 1065, 1190, 1254
MfeI CAATTG 2 cut(s) 76, 524
MhlI GDGCHC 3 cut(s) 60, 743, 940
MluCI AATT 6 cut(s) 76, 227, 235, 524, 730, 846
MlyI GAGTC 1 cut(s) 554
MnlI CCTC 9 cut(s) 107, 134, 328, 359, 467, 605, 992, 1122, 1150
Mph1103I ATGCAT 1 cut(s) 1109
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 607
MseI TTAA 1 cut(s) 861
MspA1I CMGCKG 2 cut(s) 558, 571
MspI CCGG 5 cut(s) 51, 83, 93, 203, 670
MspR9I CCNGG 3 cut(s) 51, 83, 670
MunI CAATTG 2 cut(s) 76, 524
MvnI CGCG 1 cut(s) 873
MwoI GCNNNNNNNGC 5 cut(s) 55, 60, 102, 111, 354
NciI CCSGG 3 cut(s) 51, 83, 670
NcoI CCATGG 2 cut(s) 598, 723
NdeII GATC 4 cut(s) 30, 308, 919, 1144
NlaIII CATG 5 cut(s) 273, 374, 552, 602, 727
NlaIV GGNNCC 1 cut(s) 580
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 718
NruI TCGCGA 1 cut(s) 873
NsiI ATGCAT 1 cut(s) 1109
PciSI GCTCTTC 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 607
PfeI GAWTC 6 cut(s) 117, 206, 409, 590, 603, 1219
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 3 cut(s) 48, 560, 1138
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
Ppu21I YACGTR 1 cut(s) 980
PpuMI RGGWCCY 1 cut(s) 578
PsiI TTATAA 1 cut(s) 467
Psp5II RGGWCCY 1 cut(s) 578
PspN4I GGNNCC 1 cut(s) 580
PspPI GGNCC 5 cut(s) 48, 454, 481, 578, 1001
PspPPI RGGWCCY 1 cut(s) 578
PstNI CAGNNNCTG 1 cut(s) 1010
PsyI GACNNNGTC 1 cut(s) 20
PvuII CAGCTG 2 cut(s) 558, 571
RruI TCGCGA 1 cut(s) 873
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 1 cut(s) 861
SatI GCNGC 3 cut(s) 47, 559, 1137
Sau3AI GATC 4 cut(s) 30, 308, 919, 1144
Sau96I GGNCC 5 cut(s) 48, 454, 481, 578, 1001
SchI GAGTC 1 cut(s) 554
ScrFI CCNGG 3 cut(s) 51, 83, 670
SduI GDGCHC 3 cut(s) 60, 743, 940
SfaNI GCATC 3 cut(s) 72, 954, 1148
SinI GGWCC 3 cut(s) 454, 481, 578
SmlI CTYRAG 2 cut(s) 398, 675
SmoI CTYRAG 2 cut(s) 398, 675
Sse9I AATT 6 cut(s) 76, 227, 235, 524, 730, 846
SsiI CCGC 4 cut(s) 46, 355, 949, 1136
SspI AATATT 1 cut(s) 859
StyD4I CCNGG 3 cut(s) 49, 81, 668
StyI CCWWGG 2 cut(s) 598, 723
TaaI ACNGT 4 cut(s) 73, 770, 942, 1017
TaiI ACGT 3 cut(s) 24, 341, 982
TaqI TCGA 5 cut(s) 126, 161, 351, 407, 918
TasI AATT 6 cut(s) 76, 227, 235, 524, 730, 846
TauI GCSGC 2 cut(s) 49, 1139
TfiI GAWTC 6 cut(s) 117, 206, 409, 590, 603, 1219
Tru1I TTAA 1 cut(s) 861
Tru9I TTAA 1 cut(s) 861
TscAI CASTG 3 cut(s) 282, 773, 945
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 1 cut(s) 558
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 2 cut(s) 603, 877
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 3 cut(s) 282, 773, 945
Tth111I GACNNNGTC 1 cut(s) 20
VneI GTGCAC 1 cut(s) 936
VpaK11BI GGWCC 3 cut(s) 454, 481, 578
XapI RAATTY 2 cut(s) 235, 846
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 1163
XmnI GAANNNNTTC 1 cut(s) 607
ZraI GACGTC 1 cut(s) 22
Zsp2I ATGCAT 1 cut(s) 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.