Rh5AG542500

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003332
Physical Location & Seq
Reverse (-)
93181776 .. 93188077
6302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG542500.1

Sequence Viewer

Length: 531 bp
ATGGCCAAGCTGGACCTCCACGCATCGCCACCAAGCCCAAGGAGGCCAAGCTCTAGGGCGGCCGCTTCGAGGAAGGAATCACCGACGCCGTCGAGCCCTTCACCGAGTCCATCTCCTTCGACAAAGCCCTCTACAAACATGACATCATGGGCATTAAGGCCCATGCCTCAATGCTCCCAAACAGAGAACCAGCTCCTATCCGTGTGCCCGTCATGTTCTGTTCCGTATTACTTCTACCCTCAGACTACATGTTCCTCGCCCCCTGCAGTTTATCTATTGAAGTTTCTTTGCTCTGACCGTAATGGATTATTGCATGATGTCACTGAAATTCTGTCTGATCTTGAGCTTTCGATTCAAAGGGTGAAAGTGACAACAACACCAGATGACAGGGTCTCGGACCTGTTCTTCATAACAGATAATATATGGGTAAGAGTAGTGGCTAGACATCATTGTGTTAGTATTTGGTCAGCACGTGCCTGTCCTGGAATGAAAGCTGGAAACCCAATCTATACCAAAGGGTCTTGTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

176

Amino Acids

19.31

Weight (kDa)

8.99

Isoelectric Point (pI)

74.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 92 - 139 1.2e-07 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 59, 63
AcoI YGGCCR 2 cut(s) 3, 60
AcsI RAATTY 1 cut(s) 327
AcvI CACGTG 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 86
AfaI GTAC 1 cut(s) 526
AfiI CCNNNNNNNGG 1 cut(s) 69
AflIII ACRYGT 1 cut(s) 248
AgsI TTSAA 2 cut(s) 280, 356
AjnI CCWGG 1 cut(s) 481
AluBI AGCT 5 cut(s) 10, 51, 193, 346, 494
AluI AGCT 5 cut(s) 10, 51, 193, 346, 494
Alw26I GTCTC 1 cut(s) 397
AoxI GGCC 4 cut(s) 3, 44, 60, 158
ApoI RAATTY 1 cut(s) 327
AspS9I GGNCC 3 cut(s) 13, 159, 397
AsuHPI GGTGA 3 cut(s) 72, 93, 373
AvaII GGWCC 2 cut(s) 13, 397
BaeGI GKGCMC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 98
BbrPI CACGTG 1 cut(s) 473
BccI CCATC 1 cut(s) 118
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 483
BcoDI GTCTC 1 cut(s) 397
BfaI CTAG 2 cut(s) 54, 441
BfmI CTRYAG 1 cut(s) 264
BisI GCNGC 2 cut(s) 60, 63
BlsI GCNGC 2 cut(s) 61, 64
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 2 cut(s) 13, 397
BmgT120I GGNCC 3 cut(s) 13, 159, 397
BmrFI CCNGG 1 cut(s) 483
BmsI GCATC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 97, 129
BpuEI CTTGAG 1 cut(s) 362
BsaAI YACGTR 2 cut(s) 473, 528
BsaHI GRCGYC 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 397
BsaJI CCNNGG 1 cut(s) 38
Bsc4I CCNNNNNNNGG 1 cut(s) 69
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 254
BseSI GKGCMC 1 cut(s) 209
BseX3I CGGCCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 4 cut(s) 5, 46, 62, 160
BsiEI CGRYCG 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 69
BsmAI GTCTC 1 cut(s) 397
BsnI GGCC 4 cut(s) 5, 46, 62, 160
Bso31I GGTCTC 1 cut(s) 397
Bsp1286I GDGCHC 2 cut(s) 98, 209
Bsp143I GATC 1 cut(s) 337
BspACI CCGC 2 cut(s) 59, 63
BspANI GGCC 4 cut(s) 5, 46, 62, 160
BspCNI CTCAG 1 cut(s) 253
BspMAI CTGCAG 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 397
BssECI CCNNGG 1 cut(s) 38
BssMI GATC 1 cut(s) 337
BssNI GRCGYC 1 cut(s) 86
BssT1I CCWWGG 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 1 cut(s) 299
BstACI GRCGYC 1 cut(s) 86
BstBAI YACGTR 2 cut(s) 473, 528
BstDEI CTNAG 1 cut(s) 240
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 1 cut(s) 397
BstMBI GATC 1 cut(s) 337
BstMCI CGRYCG 1 cut(s) 63
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 481
BstSFI CTRYAG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 209
BstSNI TACGTA 1 cut(s) 528
BstZI CGGCCG 1 cut(s) 60
BsuRI GGCC 4 cut(s) 5, 46, 62, 160
BtgZI GCGATG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 321
CciNI GCGGCCGC 1 cut(s) 60
Cfr13I GGNCC 3 cut(s) 13, 159, 397
CseI GACGC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 525
CviAII CATG 6 cut(s) 139, 147, 163, 213, 249, 314
CviQI GTAC 1 cut(s) 525
DdeI CTNAG 1 cut(s) 240
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
EaeI YGGCCR 2 cut(s) 3, 60
EagI CGGCCG 1 cut(s) 60
EclXI CGGCCG 1 cut(s) 60
Eco105I TACGTA 1 cut(s) 528
Eco130I CCWWGG 1 cut(s) 38
Eco24I GRGCYC 1 cut(s) 98
Eco31I GGTCTC 1 cut(s) 397
Eco47I GGWCC 2 cut(s) 13, 397
Eco52I CGGCCG 1 cut(s) 60
Eco72I CACGTG 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 1 cut(s) 38
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 6 cut(s) 142, 150, 166, 216, 252, 317
FatI CATG 6 cut(s) 138, 146, 162, 212, 248, 313
Fnu4HI GCNGC 2 cut(s) 60, 63
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 2 cut(s) 60, 63
FspBI CTAG 2 cut(s) 54, 441
GluI GCNGC 2 cut(s) 60, 63
HaeIII GGCC 4 cut(s) 5, 46, 62, 160
HgaI GACGC 1 cut(s) 94
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 6 cut(s) 142, 150, 166, 216, 252, 317
HinfI GANTC 3 cut(s) 77, 106, 352
HphI GGTGA 3 cut(s) 72, 93, 373
Hpy188I TCNGA 4 cut(s) 243, 295, 337, 397
Hpy188III TCNNGA 1 cut(s) 341
Hpy99I CGWCG 2 cut(s) 88, 94
HpyAV CCTTC 3 cut(s) 67, 108, 126
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 2 cut(s) 472, 527
HpyCH4V TGCA 2 cut(s) 266, 313
HpyF3I CTNAG 1 cut(s) 240
HpySE526I ACGT 2 cut(s) 472, 527
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 6 cut(s) 142, 150, 166, 216, 252, 317
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 2 cut(s) 179, 198
LpnPI CCDG 9 cut(s) 203, 276, 373, 393, 413, 468, 480, 490, 495
LweI GCATC 1 cut(s) 32
MaeI CTAG 2 cut(s) 54, 441
MaeII ACGT 2 cut(s) 472, 527
MaeIII GTNAC 2 cut(s) 319, 367
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 1 cut(s) 397
MhlI GDGCHC 2 cut(s) 98, 209
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 327
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 115
MnlI CCTC 7 cut(s) 26, 36, 63, 139, 177, 249, 265
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 155
MslI CAYNNNNRTG 1 cut(s) 450
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 483
NdeII GATC 1 cut(s) 337
NlaIII CATG 6 cut(s) 142, 150, 166, 216, 252, 317
NmuCI GTSAC 2 cut(s) 319, 367
NotI GCGGCCGC 1 cut(s) 60
NspI RCATGY 1 cut(s) 252
PciI ACATGT 1 cut(s) 248
PfeI GAWTC 2 cut(s) 77, 352
PflFI GACNNNGTC 2 cut(s) 88, 389
PfoI TCCNGGA 1 cut(s) 481
PkrI GCNGC 2 cut(s) 61, 64
PleI GAGTC 1 cut(s) 114
PmaCI CACGTG 1 cut(s) 473
PmlI CACGTG 1 cut(s) 473
PpsI GAGTC 1 cut(s) 114
Ppu21I YACGTR 2 cut(s) 473, 528
PscI ACATGT 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 481
PspCI CACGTG 1 cut(s) 473
PspGI CCWGG 1 cut(s) 481
PspPI GGNCC 3 cut(s) 13, 159, 397
PstI CTGCAG 1 cut(s) 268
PsyI GACNNNGTC 2 cut(s) 88, 389
RsaI GTAC 1 cut(s) 526
RsaNI GTAC 1 cut(s) 525
RseI CAYNNNNRTG 1 cut(s) 450
SaqAI TTAA 1 cut(s) 155
SatI GCNGC 2 cut(s) 60, 63
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 3 cut(s) 13, 159, 397
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 483
SduI GDGCHC 2 cut(s) 98, 209
SetI ASST 9 cut(s) 12, 18, 53, 195, 348, 402, 475, 496, 530
SfaNI GCATC 1 cut(s) 32
SfcI CTRYAG 1 cut(s) 264
SinI GGWCC 2 cut(s) 13, 397
SmiMI CAYNNNNRTG 1 cut(s) 450
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SnaBI TACGTA 1 cut(s) 528
Sse9I AATT 1 cut(s) 327
SsiI CCGC 2 cut(s) 59, 63
SspMI CTAG 2 cut(s) 54, 441
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 2 cut(s) 475, 530
TaqI TCGA 4 cut(s) 68, 92, 119, 350
TasI AATT 1 cut(s) 327
TauI GCSGC 2 cut(s) 62, 65
TfiI GAWTC 2 cut(s) 77, 352
Tru1I TTAA 1 cut(s) 155
Tru9I TTAA 1 cut(s) 155
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 2 cut(s) 319, 367
Tsp45I GTSAC 2 cut(s) 319, 367
TspDTI ATGAA 2 cut(s) 397, 503
TspGWI ACGGA 2 cut(s) 190, 213
TspRI CASTG 1 cut(s) 328
Tth111I GACNNNGTC 2 cut(s) 88, 389
VpaK11BI GGWCC 2 cut(s) 13, 397
XapI RAATTY 1 cut(s) 327
XceI RCATGY 1 cut(s) 252
XspI CTAG 2 cut(s) 54, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.