Rorug05G0358700

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003332
Physical Location & Seq
Reverse (-)
45553168 .. 45553755
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0358700.1

Sequence Viewer

Length: 588 bp
ATGGAGTGGCAATTTCACCTGTTAGAAGAGTTGTTCACAGTTGATGAAGTGAAGACTATTGCAAGTATACCACTGAGTTTGAGACAGACAGACGATCGTTTGATATGGCATTTTAATAAGAAGGGGTTGTACTCTGTGAAGAGCGGGTACCATGCACTGAGGTCTTGGAGAGGGGCTAGTAATATTGCGTCTACATCAAGTACGGCAATTGGTGGACCGCTAGGAAAGTATTGGAACATTGTATGGAAGACTAGGGTACCGCCCAAAGTTAGGGTGTTTATGTGGCACTTGCTTCGAGGTGTGCTACCTACACATACAACTTTAGCTGCAAGAATAGTAGAGTTACCTGATTTAAGGTGTGTATTCCGCATGGAAAGCTTTGAGATAGGTATTCACCTATTTAAACATTGCCCATCTTTAATTATGTTGTGGTCTTCTTCTTCCTTAAGTTTGAATGTTGTTGACCACCATGCAAGTACGTTAAATGCATGGATCTTTGATATGTTGGATAAGTTGAGGAGGGGGGGGAGCAGGTTTGTCATGCCCCTGATTTTTAACACAAATAAAAATCGATATATAACCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

195

Amino Acids

22.55

Weight (kDa)

10.01

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 44 - 144 4.2e-14 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 522
Acc65I GGTACC 2 cut(s) 147, 256
AccB1I GGYRCC 2 cut(s) 147, 256
AccBSI CCGCTC 1 cut(s) 144
AccI GTMKAC 2 cut(s) 67, 191
AciI CCGC 4 cut(s) 144, 218, 260, 367
AclWI GGATC 1 cut(s) 500
AfaI GTAC 5 cut(s) 131, 149, 202, 258, 478
AfiI CCNNNNNNNGG 1 cut(s) 270
AflII CTTAAG 1 cut(s) 445
AgsI TTSAA 1 cut(s) 454
AluBI AGCT 2 cut(s) 326, 378
AluI AGCT 2 cut(s) 326, 378
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 1 cut(s) 500
ApeKI GCWGC 1 cut(s) 326
Asp718I GGTACC 2 cut(s) 147, 256
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 8, 386
AvaII GGWCC 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 240, 273
BanI GGYRCC 2 cut(s) 147, 256
BarI GAAGNNNNNNTAC 4 cut(s) 113, 131, 145, 163
BbsI GAAGAC 3 cut(s) 59, 254, 426
BbvI GCAGC 1 cut(s) 313
BccI CCATC 1 cut(s) 421
BceAI ACGGC 1 cut(s) 219
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 3 cut(s) 177, 221, 252
BfrI CTTAAG 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 522
BisI GCNGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 328
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 2 cut(s) 149, 258
BpiI GAAGAC 3 cut(s) 59, 254, 426
Bsa29I ATCGAT 1 cut(s) 571
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 406
BseCI ATCGAT 1 cut(s) 571
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 2 cut(s) 65, 149
BseRI GAGGAG 1 cut(s) 532
BseXI GCAGC 1 cut(s) 313
Bsh1285I CGRYCG 1 cut(s) 97
BshNI GGYRCC 2 cut(s) 147, 256
BshVI ATCGAT 1 cut(s) 571
BsiEI CGRYCG 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 76
Bsp143I GATC 2 cut(s) 94, 492
BspACI CCGC 4 cut(s) 144, 218, 260, 367
BspCNI CTCAG 2 cut(s) 66, 150
BspDI ATCGAT 1 cut(s) 571
BspLI GGNNCC 2 cut(s) 149, 258
BspMI ACCTGC 1 cut(s) 522
BspPI GGATC 1 cut(s) 500
BspQI GCTCTTC 1 cut(s) 134
BspT107I GGYRCC 2 cut(s) 147, 256
BspTI CTTAAG 1 cut(s) 445
BsrBI CCGCTC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 406
BssMI GATC 2 cut(s) 94, 492
BssNAI GTATAC 1 cut(s) 68
Bst1107I GTATAC 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 40
Bst6I CTCTTC 2 cut(s) 21, 134
BstAFI CTTAAG 1 cut(s) 445
BstDEI CTNAG 2 cut(s) 74, 158
BstKTI GATC 2 cut(s) 97, 495
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 2 cut(s) 94, 492
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 313
BstV2I GAAGAC 3 cut(s) 59, 254, 426
BstX2I RGATCY 1 cut(s) 492
BstYI RGATCY 1 cut(s) 492
BstZ17I GTATAC 1 cut(s) 68
Bsu15I ATCGAT 1 cut(s) 571
BsuTUI ATCGAT 1 cut(s) 571
BtsIMutI CAGTG 2 cut(s) 71, 155
BveI ACCTGC 1 cut(s) 522
Cfr13I GGNCC 1 cut(s) 215
ClaI ATCGAT 1 cut(s) 571
CseI GACGC 1 cut(s) 177
Csp6I GTAC 5 cut(s) 130, 148, 201, 257, 477
CspCI CAANNNNNGTGG 2 cut(s) 60, 95
CviAII CATG 5 cut(s) 152, 370, 470, 489, 541
CviJI RGCY 3 cut(s) 176, 326, 378
CviKI_1 RGCY 3 cut(s) 176, 326, 378
CviQI GTAC 5 cut(s) 130, 148, 201, 257, 477
DdeI CTNAG 2 cut(s) 74, 158
DpnI GATC 2 cut(s) 96, 494
DpnII GATC 2 cut(s) 94, 492
DraI TTTAAA 1 cut(s) 403
Eam1104I CTCTTC 2 cut(s) 21, 134
EarI CTCTTC 2 cut(s) 21, 134
Eco47I GGWCC 1 cut(s) 215
EcoT22I ATGCAT 1 cut(s) 490
FaeI CATG 5 cut(s) 155, 373, 473, 492, 544
FatI CATG 5 cut(s) 151, 369, 469, 488, 540
FauI CCCGC 1 cut(s) 137
FblI GTMKAC 2 cut(s) 67, 191
Fnu4HI GCNGC 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 327
FspBI CTAG 3 cut(s) 177, 221, 252
GluI GCNGC 1 cut(s) 327
HgaI GACGC 1 cut(s) 177
Hin1II CATG 5 cut(s) 155, 373, 473, 492, 544
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 1 cut(s) 376
HphI GGTGA 2 cut(s) 8, 386
Hpy166II GTNNAC 5 cut(s) 36, 68, 192, 215, 463
Hpy8I GTNNAC 5 cut(s) 36, 68, 192, 215, 463
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4IV ACGT 1 cut(s) 479
HpyCH4V TGCA 5 cut(s) 62, 155, 329, 473, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpyF3I CTNAG 2 cut(s) 74, 158
HpySE526I ACGT 1 cut(s) 479
Hsp92II CATG 5 cut(s) 155, 373, 473, 492, 544
KpnI GGTACC 2 cut(s) 151, 260
Kzo9I GATC 2 cut(s) 94, 492
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 1 cut(s) 528
LpnPI CCDG 4 cut(s) 32, 360, 517, 560
Lsp1109I GCAGC 1 cut(s) 313
MaeI CTAG 3 cut(s) 177, 221, 252
MaeII ACGT 1 cut(s) 479
MaeIII GTNAC 1 cut(s) 342
MalI GATC 2 cut(s) 96, 494
MbiI CCGCTC 1 cut(s) 144
MboI GATC 2 cut(s) 94, 492
MboII GAAGA 7 cut(s) 38, 64, 151, 259, 426, 429, 432
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 1 cut(s) 492
MluCI AATT 3 cut(s) 11, 207, 420
MmeI TCCRAC 1 cut(s) 486
MnlI CCTC 5 cut(s) 153, 164, 290, 510, 513
Mph1103I ATGCAT 1 cut(s) 490
MseI TTAA 7 cut(s) 114, 353, 402, 419, 446, 482, 555
MspCI CTTAAG 1 cut(s) 445
MunI CAATTG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 375
NdeII GATC 2 cut(s) 94, 492
NlaIII CATG 5 cut(s) 155, 373, 473, 492, 544
NlaIV GGNNCC 2 cut(s) 149, 258
NsiI ATGCAT 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 134
PkrI GCNGC 1 cut(s) 328
Ple19I CGATCG 1 cut(s) 97
PspN4I GGNNCC 2 cut(s) 149, 258
PspPI GGNCC 1 cut(s) 215
PsuI RGATCY 1 cut(s) 492
PvuI CGATCG 1 cut(s) 97
RsaI GTAC 5 cut(s) 131, 149, 202, 258, 478
RsaNI GTAC 5 cut(s) 130, 148, 201, 257, 477
SapI GCTCTTC 1 cut(s) 134
SaqAI TTAA 7 cut(s) 114, 353, 402, 419, 446, 482, 555
SatI GCNGC 1 cut(s) 327
Sau3AI GATC 2 cut(s) 94, 492
Sau96I GGNCC 1 cut(s) 215
SinI GGWCC 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
Sse9I AATT 3 cut(s) 11, 207, 420
SsiI CCGC 4 cut(s) 144, 218, 260, 367
SspI AATATT 1 cut(s) 184
SspMI CTAG 3 cut(s) 177, 221, 252
TaaI ACNGT 1 cut(s) 40
TaiI ACGT 1 cut(s) 482
TaqI TCGA 2 cut(s) 295, 571
TasI AATT 3 cut(s) 11, 207, 420
TatI WGTACW 1 cut(s) 129
Tru1I TTAA 7 cut(s) 114, 353, 402, 419, 446, 482, 555
Tru9I TTAA 7 cut(s) 114, 353, 402, 419, 446, 482, 555
TscAI CASTG 2 cut(s) 78, 162
TseI GCWGC 1 cut(s) 326
TspDTI ATGAA 1 cut(s) 60
TspRI CASTG 2 cut(s) 78, 162
Vha464I CTTAAG 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 215
XmiI GTMKAC 2 cut(s) 67, 191
XspI CTAG 3 cut(s) 177, 221, 252
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.