Rh5DG446300

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003332
Physical Location & Seq
Reverse (-)
71246927 .. 71251823
4897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG446300.1

Sequence Viewer

Length: 966 bp
ATGGGAATCCCGAGCGACGACGTCGTTGTGTTCCAGAAAGCTAAACGTCCTGGAGAGGCTTCCGTCATCACCGTTAATTGCCCGGATAAGACCGGCCTGGGATGTGATATTTGCAGGATCATCCTCGATTTTGGGCTCTGCATCAGAAAAGCTGATTTTTCAACTGATGGGATATGGTGCTACGTTGTATTATGGGTGGTTCCTCATTCTAACTCAACAACAGTGAAATGGTCGAATTTGGAGAACCAGCTCCTATCCGTGTGCCCGTCATGTTCTGTTCCGTATTACTTCTACCCCCAGACTACATGTTCCTCGCCCCCTGCAGTTTATCTATTGAAGTTTCTTTGCTCTGACCGTAATGGATTATTGCATGATGTCACTGAAATTCTGTCTGATCTTGAGCTTTCGATTCAAAGGGTGAAAGTGACAACAACACCAGATGACAGGGTCTTGGACCTGTTCTTCATAACAGATAACATGGATCTTCTGCACACAAAGGAGCGGCAAGATAAAACATTGAAACAGTTGCAAGCCGTATTGGGTGAGTCGTGTACCAGTTGCGAACTTCAGTTGGTAGGTCCCGAGTATGATCCCCATCATGGCATTCCTTCTCTGTCTCCTGTAGTAGCTGAAGAGCTATTTAGGTGTGAGCTGTTGGATAAAGAAATCCGTTCTCAAGCTCTTAGTCCTGATATGACGAAATTGAAGAACGCTAATGTAACAATAGACAACTCATTGAGCCCAGCACATACATTACTTCAGATCCACTGTGCTGATCACAAGGGTCTCTTGTATGACATTATGAGAACTTTGAAAGACAGCAACATCAAGGTAAAGATTCTTATATCATCTTTTTTCCAGACAACATCAGATCATATTTTAGTAACTAGGCTATTTTATTGTGTTAGTTTGGTCAGCACATGCCTGATTGCCTGTCCTGGAATGGTAAAATCGCCGGCCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

321

Amino Acids

35.76

Weight (kDa)

5.7

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACR10_N PF24914 12 - 89 4.8e-40 ACT domain-containing protein ACR10, N-terminal
ACT PF01842 111 - 171 2.9e-09 ACT domain
ACT_ACR9_3rd PF24931 239 - 279 4.4e-19 ACR9-like, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccBSI CCGCTC 1 cut(s) 502
AciI CCGC 1 cut(s) 502
AclWI GGATC 4 cut(s) 125, 489, 584, 757
AcoI YGGCCR 1 cut(s) 957
AcsI RAATTY 2 cut(s) 235, 384
AcuI CTGAAG 3 cut(s) 551, 651, 743
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 6 cut(s) 162, 337, 413, 520, 706, 814
AjnI CCWGG 3 cut(s) 49, 96, 937
AluBI AGCT 8 cut(s) 41, 152, 250, 403, 629, 637, 652, 680
AluI AGCT 8 cut(s) 41, 152, 250, 403, 629, 637, 652, 680
Alw26I GTCTC 2 cut(s) 621, 791
AlwI GGATC 4 cut(s) 125, 489, 584, 757
Ama87I CYCGRG 2 cut(s) 10, 581
AoxI GGCC 2 cut(s) 94, 957
ApoI RAATTY 2 cut(s) 235, 384
AspS9I GGNCC 2 cut(s) 454, 578
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 3 cut(s) 61, 430, 554
AvaI CYCGRG 2 cut(s) 10, 581
AvaII GGWCC 2 cut(s) 454, 578
BaeGI GKGCMC 1 cut(s) 266
BanII GRGCYC 2 cut(s) 138, 743
BccI CCATC 2 cut(s) 161, 603
BceAI ACGGC 1 cut(s) 518
BciT130I CCWGG 3 cut(s) 51, 98, 939
BclI TGATCA 1 cut(s) 775
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 2 cut(s) 621, 791
BfaI CTAG 1 cut(s) 888
BfmI CTRYAG 2 cut(s) 321, 621
BisI GCNGC 1 cut(s) 503
BlsI GCNGC 1 cut(s) 504
Bme1390I CCNGG 4 cut(s) 51, 83, 98, 939
Bme18I GGWCC 2 cut(s) 454, 578
BmeT110I CYCGRG 2 cut(s) 10, 581
BmgT120I GGNCC 2 cut(s) 454, 578
BmiI GGNNCC 2 cut(s) 201, 580
BmrFI CCNGG 4 cut(s) 51, 83, 98, 939
BmsI GCATC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 2 cut(s) 419, 660
BpuMI CCSGG 1 cut(s) 83
BsaBI GATNNNNATC 1 cut(s) 594
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 791
BsaJI CCNNGG 1 cut(s) 97
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse118I RCCGGY 2 cut(s) 92, 955
Bse1I ACTGG 1 cut(s) 555
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 3 cut(s) 51, 98, 939
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 2 cut(s) 107, 120
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 1 cut(s) 599
BseNI ACTGG 1 cut(s) 555
BseSI GKGCMC 1 cut(s) 266
BseYI CCCAGC 1 cut(s) 742
BsgI GTGCAG 1 cut(s) 473
BshFI GGCC 2 cut(s) 96, 959
BsiHKCI CYCGRG 2 cut(s) 10, 581
BsiSI CCGG 3 cut(s) 83, 93, 956
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 2 cut(s) 621, 791
BsmFI GGGAC 1 cut(s) 564
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 2 cut(s) 96, 959
Bso31I GGTCTC 1 cut(s) 791
BsoBI CYCGRG 2 cut(s) 10, 581
Bsp1286I GDGCHC 3 cut(s) 138, 266, 743
Bsp143I GATC 7 cut(s) 117, 394, 481, 589, 762, 775, 871
BspACI CCGC 1 cut(s) 502
BspANI GGCC 2 cut(s) 96, 959
BspLI GGNNCC 2 cut(s) 201, 580
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 4 cut(s) 125, 489, 584, 757
BspQI GCTCTTC 1 cut(s) 627
BspTNI GGTCTC 1 cut(s) 791
BsrBI CCGCTC 1 cut(s) 502
BsrFI RCCGGY 2 cut(s) 92, 955
BsrI ACTGG 1 cut(s) 555
BssAI RCCGGY 2 cut(s) 92, 955
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 7 cut(s) 117, 394, 481, 589, 762, 775, 871
BssNI GRCGYC 1 cut(s) 21
Bst2UI CCWGG 3 cut(s) 51, 98, 939
Bst4CI ACNGT 5 cut(s) 73, 223, 356, 525, 770
Bst6I CTCTTC 1 cut(s) 627
BstACI GRCGYC 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 531, 957
BstDEI CTNAG 1 cut(s) 683
BstF5I GGATG 2 cut(s) 107, 120
BstKTI GATC 7 cut(s) 120, 397, 484, 592, 765, 778, 874
BstMAI GTCTC 2 cut(s) 621, 791
BstMBI GATC 7 cut(s) 117, 394, 481, 589, 762, 775, 871
BstNI CCWGG 3 cut(s) 51, 98, 939
BstNSI RCATGY 2 cut(s) 309, 924
BstSCI CCNGG 4 cut(s) 49, 81, 96, 937
BstSFI CTRYAG 2 cut(s) 321, 621
BstSLI GKGCMC 1 cut(s) 266
BstX2I RGATCY 2 cut(s) 481, 762
BstYI RGATCY 2 cut(s) 481, 762
BsuRI GGCC 2 cut(s) 96, 959
BtsCI GGATG 2 cut(s) 107, 120
BtsIMutI CAGTG 3 cut(s) 228, 378, 766
Cac8I GCNNGC 2 cut(s) 531, 957
Cfr10I RCCGGY 2 cut(s) 92, 955
Cfr13I GGNCC 2 cut(s) 454, 578
Csp6I GTAC 1 cut(s) 552
CviAII CATG 6 cut(s) 270, 306, 371, 478, 599, 921
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 1 cut(s) 683
DpnI GATC 7 cut(s) 119, 396, 483, 591, 764, 777, 873
DpnII GATC 7 cut(s) 117, 394, 481, 589, 762, 775, 871
EaeI YGGCCR 1 cut(s) 957
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Eco24I GRGCYC 2 cut(s) 138, 743
Eco31I GGTCTC 1 cut(s) 791
Eco47I GGWCC 2 cut(s) 454, 578
Eco57I CTGAAG 3 cut(s) 551, 651, 743
Eco88I CYCGRG 2 cut(s) 10, 581
EcoO109I RGGNCCY 1 cut(s) 578
EcoRII CCWGG 3 cut(s) 49, 96, 937
EcoT38I GRGCYC 2 cut(s) 138, 743
FaeI CATG 6 cut(s) 273, 309, 374, 481, 602, 924
FalI AAGNNNNNCTT 2 cut(s) 773, 805
FaqI GGGAC 1 cut(s) 564
FatI CATG 6 cut(s) 269, 305, 370, 477, 598, 920
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 1 cut(s) 503
FokI GGATG 2 cut(s) 107, 114
FriOI GRGCYC 2 cut(s) 138, 743
Fsp4HI GCNGC 1 cut(s) 503
FspBI CTAG 1 cut(s) 888
GluI GCNGC 1 cut(s) 503
GsaI CCCAGC 1 cut(s) 746
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 2 cut(s) 96, 959
HapII CCGG 3 cut(s) 83, 93, 956
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 6 cut(s) 273, 309, 374, 481, 602, 924
HinfI GANTC 4 cut(s) 6, 409, 545, 838
HpaII CCGG 3 cut(s) 83, 93, 956
HphI GGTGA 3 cut(s) 61, 430, 554
Hpy166II GTNNAC 1 cut(s) 552
Hpy188I TCNGA 5 cut(s) 146, 352, 394, 762, 871
Hpy188III TCNNGA 6 cut(s) 10, 34, 398, 581, 689, 859
Hpy8I GTNNAC 1 cut(s) 552
Hpy99I CGWCG 3 cut(s) 20, 23, 26
HpyAV CCTTC 1 cut(s) 618
HpyCH4III ACNGT 5 cut(s) 73, 223, 356, 525, 770
HpyCH4IV ACGT 3 cut(s) 21, 46, 183
HpyCH4V TGCA 6 cut(s) 114, 141, 323, 370, 490, 529
HpyF3I CTNAG 1 cut(s) 683
HpySE526I ACGT 3 cut(s) 21, 46, 183
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 6 cut(s) 273, 309, 374, 481, 602, 924
KroI GCCGGC 1 cut(s) 955
KroNI GCCGGC 1 cut(s) 957
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 7 cut(s) 117, 394, 481, 589, 762, 775, 871
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 2 cut(s) 255, 499
LweI GCATC 1 cut(s) 150
MaeI CTAG 1 cut(s) 888
MaeII ACGT 3 cut(s) 21, 46, 183
MaeIII GTNAC 4 cut(s) 376, 424, 718, 883
MalI GATC 7 cut(s) 119, 396, 483, 591, 764, 777, 873
MbiI CCGCTC 1 cut(s) 502
MboI GATC 7 cut(s) 117, 394, 481, 589, 762, 775, 871
MboII GAAGA 4 cut(s) 454, 476, 644, 718
MflI RGATCY 2 cut(s) 481, 762
MhlI GDGCHC 3 cut(s) 138, 266, 743
MluCI AATT 4 cut(s) 76, 235, 384, 701
MlyI GAGTC 1 cut(s) 554
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 4 cut(s) 49, 134, 213, 322
MroNI GCCGGC 1 cut(s) 955
MseI TTAA 1 cut(s) 75
MspI CCGG 3 cut(s) 83, 93, 956
MspR9I CCNGG 4 cut(s) 51, 83, 98, 939
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 3 cut(s) 51, 98, 939
NaeI GCCGGC 1 cut(s) 957
NciI CCSGG 1 cut(s) 83
NdeII GATC 7 cut(s) 117, 394, 481, 589, 762, 775, 871
NgoMIV GCCGGC 1 cut(s) 955
NlaIII CATG 6 cut(s) 273, 309, 374, 481, 602, 924
NlaIV GGNNCC 2 cut(s) 201, 580
NmuCI GTSAC 2 cut(s) 376, 424
NspI RCATGY 2 cut(s) 309, 924
PciI ACATGT 1 cut(s) 305
PciSI GCTCTTC 1 cut(s) 627
PctI GAATGC 1 cut(s) 603
PdiI GCCGGC 1 cut(s) 957
PfeI GAWTC 3 cut(s) 6, 409, 838
PflFI GACNNNGTC 2 cut(s) 20, 446
PfoI TCCNGGA 2 cut(s) 49, 937
PkrI GCNGC 1 cut(s) 504
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
PpuMI RGGWCCY 1 cut(s) 578
PscI ACATGT 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 578
Psp6I CCWGG 3 cut(s) 49, 96, 937
PspFI CCCAGC 1 cut(s) 742
PspGI CCWGG 3 cut(s) 49, 96, 937
PspN4I GGNNCC 2 cut(s) 201, 580
PspPI GGNCC 2 cut(s) 454, 578
PspPPI RGGWCCY 1 cut(s) 578
PstI CTGCAG 1 cut(s) 325
PsuI RGATCY 2 cut(s) 481, 762
PsyI GACNNNGTC 2 cut(s) 20, 446
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 1 cut(s) 503
Sau3AI GATC 7 cut(s) 117, 394, 481, 589, 762, 775, 871
Sau96I GGNCC 2 cut(s) 454, 578
SchI GAGTC 1 cut(s) 554
ScrFI CCNGG 4 cut(s) 51, 83, 98, 939
SduI GDGCHC 3 cut(s) 138, 266, 743
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 2 cut(s) 321, 621
SinI GGWCC 2 cut(s) 454, 578
SmlI CTYRAG 2 cut(s) 398, 675
SmoI CTYRAG 2 cut(s) 398, 675
Sse9I AATT 4 cut(s) 76, 235, 384, 701
SsiI CCGC 1 cut(s) 502
SspMI CTAG 1 cut(s) 888
StyD4I CCNGG 4 cut(s) 49, 81, 96, 937
TaaI ACNGT 5 cut(s) 73, 223, 356, 525, 770
TaiI ACGT 3 cut(s) 24, 49, 186
TaqI TCGA 3 cut(s) 126, 233, 407
TasI AATT 4 cut(s) 76, 235, 384, 701
TauI GCSGC 1 cut(s) 505
TfiI GAWTC 3 cut(s) 6, 409, 838
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 3 cut(s) 228, 385, 773
TseFI GTSAC 2 cut(s) 376, 424
Tsp45I GTSAC 2 cut(s) 376, 424
TspDTI ATGAA 1 cut(s) 454
TspGWI ACGGA 4 cut(s) 52, 247, 270, 659
TspRI CASTG 3 cut(s) 228, 385, 773
Tth111I GACNNNGTC 2 cut(s) 20, 446
VpaK11BI GGWCC 2 cut(s) 454, 578
XapI RAATTY 2 cut(s) 235, 384
XceI RCATGY 2 cut(s) 309, 924
XspI CTAG 1 cut(s) 888
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.