FvH4_7g22000

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003332
Physical Location & Seq
Reverse (-)
17472159 .. 17475581
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22000.t1

Sequence Viewer

Length: 1209 bp
ATGGGCATTTCCTGCGACGACGAAGACGTCGTGATAATCCAGCAGGGGAAGAGCCCCGGCGACCCGACCGTCGTGACAGTCAATTGTCCCGACGAGCCCGGGCTCGGCTGCGACCTCTGCAGGATCGTCCTTCAATTCGGGCTTTGCATTAGCAAAGGAGATTTCACGACGGACGGGAGGTGGTGCTATATAGTGTTGTGGGTGGTGCCGAACGAGAGCTCGATGAAGGTGGATTGGGACAGCTTGAAGGAGAGGCTCCTGTCGGCGTGTCCGTCTTCGTGTTTCTATAAGGTTTACCTGAATATTCAGACCAGTGCTCCGTCGCCGTCGCCGGTTTACTTGTTGAAGTTTTGGTGCTTTGACCAGAGGGGGTCTCTGCATGATGTTACTAAGGTGCTGTGTGAGCTTGAGATTCTGATTCAGAGAGTGAAAGTTATGCCAACGCCGGATGGGAGGGTTTTGGACCTGTTCTTCATCACTGATTGCTTAGATTTGCTGCACACACAAGAAAGGAGAGATCATATATGTGAACATCTGATGGCTGCCCTAGGAGAGTATTGCATCACCTGTGAGGTTCAGTTGGCAGGGCCTGAATATGAAAGTTTGCAGGGAAATTATTCTCTTCCGTCAGCTATTGCTGAAGAGTTATTTAGCTATCTGCCAACAGATGACAAAGCTTCTTCAAAAGCTCTCAGCCCGAGTTCGGCAACTTTGAAAAACACAAGTATTATGGTGGATAATACACTGAGCCCGGTTCATACATTGCTGAAAATACAATGTGTGGATCAGAAAGGCCTCTTTTATGACATACTAAGGACGTCCAAGGATTGCAACATTCAGCACAAGGATGGGAAAAAGATTTTAGATCCTGAAAGACAGGTTGCATTGTGTTCTCGGTTGAAAGAGCAGATGCTTCACCCACTGAGAGTGATTGTTGCCAACAAGGGTCCGGATACTGAACTCCTAGTCGCTAATCCAGTTGAGTTATCTGGAAAAGGACGGCCCCTCGTCTTTCATGATGTTACTCTTGCACTGAAAAAGTTGGGATTATCCATTTTCTTGGCTGAAATTGTAAGGCATGCGACTCAAGATTGCCAGTGGGAAGTCTATAGGTTTCTTTTGGATGATAGTCGTGAATTTCCTTTGGGGAGCACAGAAGCTAGAAATAAGATCGAACAAAGAGTTAGAAGAACATTAATGGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

403

Amino Acids

45.23

Weight (kDa)

5.47

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACR10_N PF24914 13 - 91 1.1e-40 ACT domain-containing protein ACR10, N-terminal
ACT_ACR9_3rd PF24931 242 - 280 2.2e-16 ACR9-like, ACT-like domain
ACT_ACR9_3rd PF24931 279 - 318 2e-12 ACR9-like, ACT-like domain
ACT_ACR9_C PF24926 319 - 401 4.9e-37 ACT domain-containing protein ACR9, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 26, 68
AatII GACGTC 2 cut(s) 30, 821
AccB1I GGYRCC 1 cut(s) 205
AccIII TCCGGA 1 cut(s) 949
AclWI GGATC 3 cut(s) 131, 792, 860
AcsI RAATTY 1 cut(s) 1136
AcuI CTGAAG 1 cut(s) 660
AcyI GRCGYC 2 cut(s) 27, 818
AfiI CCNNNNNNNGG 2 cut(s) 104, 703
AgsI TTSAA 6 cut(s) 134, 247, 346, 684, 715, 901
AluBI AGCT 8 cut(s) 219, 243, 406, 632, 654, 677, 689, 1160
AluI AGCT 8 cut(s) 219, 243, 406, 632, 654, 677, 689, 1160
Alw21I GWGCWC 3 cut(s) 221, 319, 1154
Alw26I GTCTC 1 cut(s) 378
AlwI GGATC 3 cut(s) 131, 792, 860
AlwNI CAGNNNCTG 1 cut(s) 590
Ama87I CYCGRG 2 cut(s) 98, 697
Aor13HI TCCGGA 1 cut(s) 949
AoxI GGCC 3 cut(s) 587, 793, 1001
ApeKI GCWGC 3 cut(s) 108, 496, 542
ApoI RAATTY 1 cut(s) 1136
AseI ATTAAT 1 cut(s) 1196
Asp700I GAANNNNTTC 1 cut(s) 616
AspA2I CCTAGG 1 cut(s) 547
AspS9I GGNCC 4 cut(s) 463, 587, 947, 1002
AsuC2I CCSGG 4 cut(s) 57, 99, 100, 752
AsuHPI GGTGA 2 cut(s) 556, 908
AvaI CYCGRG 2 cut(s) 98, 697
AvaII GGWCC 2 cut(s) 463, 947
AvrII CCTAGG 1 cut(s) 547
BanI GGYRCC 1 cut(s) 205
BanII GRGCYC 5 cut(s) 56, 99, 105, 221, 752
BbsI GAAGAC 2 cut(s) 30, 267
Bbv12I GWGCWC 3 cut(s) 221, 319, 1154
BbvI GCAGC 3 cut(s) 95, 483, 529
BccI CCATC 4 cut(s) 443, 532, 842, 1197
BceAI ACGGC 2 cut(s) 310, 1016
BciVI GTATCC 1 cut(s) 946
BcnI CCSGG 4 cut(s) 57, 99, 100, 752
BcoDI GTCTC 1 cut(s) 378
BfaI CTAG 3 cut(s) 548, 965, 1161
BfmI CTRYAG 2 cut(s) 118, 1108
BfuI GTATCC 1 cut(s) 946
BisI GCNGC 3 cut(s) 109, 497, 543
BlnI CCTAGG 1 cut(s) 547
BlsI GCNGC 3 cut(s) 110, 498, 544
Bme1390I CCNGG 4 cut(s) 57, 99, 100, 752
Bme18I GGWCC 2 cut(s) 463, 947
BmeT110I CYCGRG 2 cut(s) 98, 697
BmgT120I GGNCC 4 cut(s) 463, 587, 947, 1002
BmiI GGNNCC 4 cut(s) 207, 257, 948, 1004
BmrFI CCNGG 4 cut(s) 57, 99, 100, 752
BmsI GCATC 2 cut(s) 570, 900
BpiI GAAGAC 2 cut(s) 30, 267
BplI GAGNNNNNCTC 2 cut(s) 358, 390
BpuEI CTTGAG 2 cut(s) 428, 1071
BpuMI CCSGG 4 cut(s) 57, 99, 100, 752
BsaHI GRCGYC 2 cut(s) 27, 818
BsaI GGTCTC 1 cut(s) 378
BsaJI CCNNGG 4 cut(s) 55, 98, 547, 822
BsaWI WCCGGW 1 cut(s) 949
BsaXI ACNNNNNCTCC 2 cut(s) 301, 331
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 703
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 3 cut(s) 312, 977, 1096
Bse3DI GCAATG 1 cut(s) 761
BseAI TCCGGA 1 cut(s) 949
BseDI CCNNGG 4 cut(s) 55, 98, 547, 822
BseGI GGATG 4 cut(s) 454, 853, 1129, 1208
BseLI CCNNNNNNNGG 2 cut(s) 104, 703
BseMI GCAATG 1 cut(s) 761
BseMII CTCAG 3 cut(s) 706, 737, 914
BseNI ACTGG 3 cut(s) 312, 977, 1096
BseXI GCAGC 3 cut(s) 95, 483, 529
BsgI GTGCAG 1 cut(s) 482
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 3 cut(s) 589, 795, 1003
BshNI GGYRCC 1 cut(s) 205
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 3 cut(s) 221, 319, 1154
BsiHKCI CYCGRG 2 cut(s) 98, 697
BsiSI CCGG 6 cut(s) 57, 99, 332, 446, 752, 950
BslFI GGGAC 2 cut(s) 72, 251
BslI CCNNNNNNNGG 2 cut(s) 104, 703
BsmAI GTCTC 1 cut(s) 378
BsmFI GGGAC 2 cut(s) 72, 251
BsnI GGCC 3 cut(s) 589, 795, 1003
Bso31I GGTCTC 1 cut(s) 378
BsoBI CYCGRG 2 cut(s) 98, 697
Bsp1286I GDGCHC 7 cut(s) 56, 99, 105, 221, 319, 752, 1154
Bsp13I TCCGGA 1 cut(s) 949
Bsp143I GATC 5 cut(s) 123, 517, 784, 865, 1170
BspANI GGCC 3 cut(s) 589, 795, 1003
BspCNI CTCAG 3 cut(s) 705, 738, 915
BspEI TCCGGA 1 cut(s) 949
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 4 cut(s) 207, 257, 948, 1004
BspMAI CTGCAG 1 cut(s) 122
BspPI GGATC 3 cut(s) 131, 792, 860
BspQI GCTCTTC 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 205
BspTNI GGTCTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 761
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 3 cut(s) 312, 977, 1096
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 4 cut(s) 55, 98, 547, 822
BssMI GATC 5 cut(s) 123, 517, 784, 865, 1170
BssNI GRCGYC 2 cut(s) 27, 818
BssT1I CCWWGG 2 cut(s) 547, 822
Bst4CI ACNGT 2 cut(s) 70, 79
Bst6I CTCTTC 3 cut(s) 44, 627, 636
BstACI GRCGYC 2 cut(s) 27, 818
BstAPI GCANNNNNTGC 1 cut(s) 12
BstC8I GCNNGC 1 cut(s) 1080
BstDEI CTNAG 6 cut(s) 390, 487, 692, 746, 812, 923
BstF5I GGATG 4 cut(s) 454, 853, 1129, 1208
BstKTI GATC 5 cut(s) 126, 520, 787, 868, 1173
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 5 cut(s) 123, 517, 784, 865, 1170
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 3 cut(s) 12, 117, 403
BstNSI RCATGY 1 cut(s) 1082
BstSCI CCNGG 4 cut(s) 55, 97, 98, 750
BstSFI CTRYAG 2 cut(s) 118, 1108
BstV1I GCAGC 3 cut(s) 95, 483, 529
BstV2I GAAGAC 2 cut(s) 30, 267
BstX2I RGATCY 1 cut(s) 865
BstXI CCANNNNNNTGG 1 cut(s) 1060
BstYI RGATCY 1 cut(s) 865
BsuI GTATCC 1 cut(s) 946
BsuRI GGCC 3 cut(s) 589, 795, 1003
BtsCI GGATG 4 cut(s) 454, 853, 1129, 1208
BtsIMutI CAGTG 6 cut(s) 319, 477, 743, 920, 1031, 1103
Cac8I GCNNGC 1 cut(s) 1080
CaiI CAGNNNCTG 1 cut(s) 590
CciI TCATGA 1 cut(s) 1015
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 4 cut(s) 463, 587, 947, 1002
Cfr9I CCCGGG 1 cut(s) 98
CviAII CATG 3 cut(s) 380, 1016, 1079
DdeI CTNAG 6 cut(s) 390, 487, 692, 746, 812, 923
DpnI GATC 5 cut(s) 125, 519, 786, 867, 1172
DpnII GATC 5 cut(s) 123, 517, 784, 865, 1170
DrdI GACNNNNNNGTC 2 cut(s) 26, 68
DseDI GACNNNNNNGTC 2 cut(s) 26, 68
Eam1104I CTCTTC 3 cut(s) 44, 627, 636
EarI CTCTTC 3 cut(s) 44, 627, 636
Ecl136II GAGCTC 1 cut(s) 219
Eco130I CCWWGG 2 cut(s) 547, 822
Eco147I AGGCCT 1 cut(s) 795
Eco24I GRGCYC 5 cut(s) 56, 99, 105, 221, 752
Eco31I GGTCTC 1 cut(s) 378
Eco47I GGWCC 2 cut(s) 463, 947
Eco53kI GAGCTC 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 660
Eco88I CYCGRG 2 cut(s) 98, 697
EcoICRI GAGCTC 1 cut(s) 219
EcoO109I RGGNCCY 1 cut(s) 587
EcoT14I CCWWGG 2 cut(s) 547, 822
EcoT38I GRGCYC 5 cut(s) 56, 99, 105, 221, 752
ErhI CCWWGG 2 cut(s) 547, 822
FaeI CATG 3 cut(s) 383, 1019, 1082
FaqI GGGAC 2 cut(s) 72, 251
FatI CATG 3 cut(s) 379, 1015, 1078
Fnu4HI GCNGC 3 cut(s) 109, 497, 543
FokI GGATG 3 cut(s) 461, 860, 1136
FriOI GRGCYC 5 cut(s) 56, 99, 105, 221, 752
Fsp4HI GCNGC 3 cut(s) 109, 497, 543
FspBI CTAG 3 cut(s) 548, 965, 1161
GluI GCNGC 3 cut(s) 109, 497, 543
HaeIII GGCC 3 cut(s) 589, 795, 1003
HapII CCGG 6 cut(s) 57, 99, 332, 446, 752, 950
Hin1I GRCGYC 2 cut(s) 27, 818
Hin1II CATG 3 cut(s) 383, 1019, 1082
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 3 cut(s) 412, 418, 1084
HpaII CCGG 6 cut(s) 57, 99, 332, 446, 752, 950
HphI GGTGA 2 cut(s) 556, 908
Hpy166II GTNNAC 3 cut(s) 295, 337, 530
Hpy188I TCNGA 5 cut(s) 309, 417, 423, 537, 789
Hpy8I GTNNAC 3 cut(s) 295, 337, 530
Hpy99I CGWCG 8 cut(s) 20, 23, 32, 74, 95, 172, 325, 331
HpyAV CCTTC 3 cut(s) 140, 220, 241
HpyCH4III ACNGT 2 cut(s) 70, 79
HpyCH4IV ACGT 2 cut(s) 27, 818
HpyCH4V TGCA 9 cut(s) 120, 147, 379, 499, 561, 607, 831, 884, 1031
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 117, 403
HpyF3I CTNAG 6 cut(s) 390, 487, 692, 746, 812, 923
HpySE526I ACGT 2 cut(s) 27, 818
Hsp92I GRCGYC 2 cut(s) 27, 818
Hsp92II CATG 3 cut(s) 383, 1019, 1082
Kpn2I TCCGGA 1 cut(s) 949
Kzo9I GATC 5 cut(s) 123, 517, 784, 865, 1170
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 3 cut(s) 261, 322, 1149
Lsp1109I GCAGC 3 cut(s) 95, 483, 529
LweI GCATC 2 cut(s) 570, 900
MaeI CTAG 3 cut(s) 548, 965, 1161
MaeII ACGT 2 cut(s) 27, 818
MaeIII GTNAC 3 cut(s) 73, 385, 1021
MalI GATC 5 cut(s) 125, 519, 786, 867, 1172
MboI GATC 5 cut(s) 123, 517, 784, 865, 1170
MboII GAAGA 8 cut(s) 35, 61, 267, 463, 614, 653, 672, 1200
MfeI CAATTG 1 cut(s) 82
MflI RGATCY 1 cut(s) 865
MhlI GDGCHC 7 cut(s) 56, 99, 105, 221, 319, 752, 1154
MluCI AATT 5 cut(s) 82, 134, 613, 1068, 1136
MlyI GAGTC 1 cut(s) 1078
MnlI CCTC 8 cut(s) 125, 171, 246, 360, 447, 565, 806, 1016
MroI TCCGGA 1 cut(s) 949
MroXI GAANNNNTTC 1 cut(s) 616
MseI TTAA 1 cut(s) 1196
MslI CAYNNNNRTG 2 cut(s) 525, 846
MspI CCGG 6 cut(s) 57, 99, 332, 446, 752, 950
MspR9I CCNGG 4 cut(s) 57, 99, 100, 752
MunI CAATTG 1 cut(s) 82
MwoI GCNNNNNNNGC 3 cut(s) 12, 117, 403
NciI CCSGG 4 cut(s) 57, 99, 100, 752
NdeII GATC 5 cut(s) 123, 517, 784, 865, 1170
NlaIII CATG 3 cut(s) 383, 1019, 1082
NlaIV GGNNCC 4 cut(s) 207, 257, 948, 1004
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 73
NspI RCATGY 1 cut(s) 1082
PaeI GCATGC 1 cut(s) 1082
PagI TCATGA 1 cut(s) 1015
PceI AGGCCT 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 27, 269
PdmI GAANNNNTTC 1 cut(s) 616
PfeI GAWTC 2 cut(s) 412, 418
PkrI GCNGC 3 cut(s) 110, 498, 544
PleI GAGTC 1 cut(s) 1078
PpsI GAGTC 1 cut(s) 1078
PshBI ATTAAT 1 cut(s) 1196
Psp124BI GAGCTC 1 cut(s) 221
PspN4I GGNNCC 4 cut(s) 207, 257, 948, 1004
PspPI GGNCC 4 cut(s) 463, 587, 947, 1002
PstI CTGCAG 1 cut(s) 122
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 1 cut(s) 865
RseI CAYNNNNRTG 2 cut(s) 525, 846
SacI GAGCTC 1 cut(s) 221
SapI GCTCTTC 1 cut(s) 44
SaqAI TTAA 1 cut(s) 1196
SatI GCNGC 3 cut(s) 109, 497, 543
Sau3AI GATC 5 cut(s) 123, 517, 784, 865, 1170
Sau96I GGNCC 4 cut(s) 463, 587, 947, 1002
SchI GAGTC 1 cut(s) 1078
ScrFI CCNGG 4 cut(s) 57, 99, 100, 752
SduI GDGCHC 7 cut(s) 56, 99, 105, 221, 319, 752, 1154
SfaNI GCATC 2 cut(s) 570, 900
SfcI CTRYAG 2 cut(s) 118, 1108
SinI GGWCC 2 cut(s) 463, 947
SmaI CCCGGG 1 cut(s) 100
SmiMI CAYNNNNRTG 2 cut(s) 525, 846
SmlI CTYRAG 2 cut(s) 407, 1086
SmoI CTYRAG 2 cut(s) 407, 1086
SphI GCATGC 1 cut(s) 1082
SrfI GCCCGGGC 1 cut(s) 100
Sse9I AATT 5 cut(s) 82, 134, 613, 1068, 1136
SseBI AGGCCT 1 cut(s) 795
SspI AATATT 1 cut(s) 304
SspMI CTAG 3 cut(s) 548, 965, 1161
SstI GAGCTC 1 cut(s) 221
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 4 cut(s) 55, 97, 98, 750
StyI CCWWGG 2 cut(s) 547, 822
TaaI ACNGT 2 cut(s) 70, 79
TaiI ACGT 2 cut(s) 30, 821
TaqI TCGA 2 cut(s) 221, 1173
TasI AATT 5 cut(s) 82, 134, 613, 1068, 1136
TfiI GAWTC 2 cut(s) 412, 418
Tru1I TTAA 1 cut(s) 1196
Tru9I TTAA 1 cut(s) 1196
TscAI CASTG 6 cut(s) 319, 484, 750, 927, 1038, 1103
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 3 cut(s) 108, 496, 542
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 5 cut(s) 239, 463, 612, 746, 1004
TspGWI ACGGA 4 cut(s) 185, 261, 309, 615
TspMI CCCGGG 1 cut(s) 98
TspRI CASTG 6 cut(s) 319, 484, 750, 927, 1038, 1103
VpaK11BI GGWCC 2 cut(s) 463, 947
VspI ATTAAT 1 cut(s) 1196
XapI RAATTY 1 cut(s) 1136
XceI RCATGY 1 cut(s) 1082
XmaI CCCGGG 1 cut(s) 98
XmaJI CCTAGG 1 cut(s) 547
XmnI GAANNNNTTC 1 cut(s) 616
XspI CTAG 3 cut(s) 548, 965, 1161
ZraI GACGTC 2 cut(s) 28, 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.