FvH4_3g40200

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003044
Physical Location & Seq
Reverse (-)
33978072 .. 33979723
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40200.t2

Sequence Viewer

Length: 753 bp
ATGTCCAGAGACAGCAGGGAGATCATAAGATTGGAAGAGATTATAGGAAAGAGTATCATTAGGAGAGAAAGTGATATTCATATCACTTCTGCAGCTGATCAAATGGTAATGATGAGTCGACAGAACAACTCATTTGGTCAACTTCCTGGGATGACAACTTTGAACACAATCACACCTTGTGCTGCATGTAAGCTCTTGAGAAGAAGATGTGCTGAAGAATGCCCTTTCTCTCCCTATTTCTCCCCACATGAACCTCACAAATTTGCATCTGTCCACAAAGTCTTTGGTGCAAGCAATGTCTCCAAGTTGCTAATGGAGGTGCCAGAAAACCAAAGAGCTGATGCTGCAAATAGTCTGGTTTATGAAGCAAACCTGAGGATAAGAGACCCTGTGTACGGATGCATGGGTGTAATTTCAGACTTGCAGCAAAAAGTTCAGTCTTTACAAGCAGAACTTCATGCTGTCAGGACAGAAATTGTGAGGTACAGATATAAAGAATTAGCAGCTAGTCAAATTCTTTCTTCTAGAGATCAGATTCATCCCCATCATGCTGCTGCTAGTTTAGTTTCTTCTGGGATGGTGTCTATTGCTGCACCACCACAACCAATTTCGACATCACTACCGGCTGTTCCTCCATCTTCGGTTGCATTTTCTTCGTCTTCTTCAACTTCAACTTCTTCTTTATATACACCCTCGACAAACACAACGGGTTATAGTTCCTTGTCAAGCGAGAACATCCCGTATTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

251

Amino Acids

27.41

Weight (kDa)

7.71

Isoelectric Point (pI)

67.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 58 - 155 3.4e-40 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 319
AccI GTMKAC 1 cut(s) 118
AcsI RAATTY 2 cut(s) 260, 513
AcuI CTGAAG 1 cut(s) 234
AdeI CACNNNGTG 1 cut(s) 179
AfaI GTAC 2 cut(s) 395, 485
AfiI CCNNNNNNNGG 1 cut(s) 395
AgsI TTSAA 3 cut(s) 163, 666, 672
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 4 cut(s) 95, 193, 338, 506
AluI AGCT 4 cut(s) 95, 193, 338, 506
Alw26I GTCTC 3 cut(s) 3, 304, 378
ApeKI GCWGC 8 cut(s) 92, 182, 344, 424, 503, 551, 554, 590
ApoI RAATTY 2 cut(s) 260, 513
AxyI CCTNAGG 1 cut(s) 374
BanI GGYRCC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 651
BbvI GCAGC 8 cut(s) 104, 169, 331, 436, 515, 538, 541, 577
BccI CCATC 3 cut(s) 552, 571, 643
BcgI CGANNNNNNTGC 2 cut(s) 636, 670
BciT130I CCWGG 1 cut(s) 147
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 3 cut(s) 3, 304, 378
BfaI CTAG 3 cut(s) 507, 525, 558
BfmI CTRYAG 1 cut(s) 90
BisI GCNGC 8 cut(s) 93, 183, 345, 425, 504, 552, 555, 591
BlsI GCNGC 8 cut(s) 94, 184, 346, 426, 505, 553, 556, 592
Bme1390I CCNGG 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 3 cut(s) 275, 331, 389
BpiI GAAGAC 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 217
BsaI GGTCTC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 146
BsaXI ACNNNNNCTCC 2 cut(s) 55, 85
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse118I RCCGGY 1 cut(s) 622
Bse21I CCTNAGG 1 cut(s) 374
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 5 cut(s) 156, 404, 538, 582, 735
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 1 cut(s) 365
BseXI GCAGC 8 cut(s) 104, 169, 331, 436, 515, 538, 541, 577
BsgI GTGCAG 1 cut(s) 576
BshNI GGYRCC 1 cut(s) 319
BsiSI CCGG 1 cut(s) 623
BslI CCNNNNNNNGG 1 cut(s) 395
BsmAI GTCTC 3 cut(s) 3, 304, 378
BsmI GAATGC 1 cut(s) 224
Bso31I GGTCTC 1 cut(s) 378
Bsp143I GATC 3 cut(s) 21, 97, 529
BspCNI CTCAG 1 cut(s) 366
BspLI GGNNCC 1 cut(s) 321
BspMAI CTGCAG 1 cut(s) 94
BspT107I GGYRCC 1 cut(s) 319
BspTNI GGTCTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 622
BssAI RCCGGY 1 cut(s) 622
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 3 cut(s) 21, 97, 529
Bst2UI CCWGG 1 cut(s) 147
Bst6I CTCTTC 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 292
BstDEI CTNAG 1 cut(s) 374
BstF5I GGATG 5 cut(s) 156, 404, 538, 582, 735
BstKTI GATC 3 cut(s) 24, 100, 532
BstMAI GTCTC 3 cut(s) 3, 304, 378
BstMBI GATC 3 cut(s) 21, 97, 529
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 1 cut(s) 90
BstV1I GCAGC 8 cut(s) 104, 169, 331, 436, 515, 538, 541, 577
BstV2I GAAGAC 1 cut(s) 651
Bsu36I CCTNAGG 1 cut(s) 374
BtsCI GGATG 5 cut(s) 156, 404, 538, 582, 735
Cac8I GCNNGC 1 cut(s) 292
Cfr10I RCCGGY 1 cut(s) 622
Csp6I GTAC 2 cut(s) 394, 484
CviAII CATG 5 cut(s) 186, 248, 403, 458, 548
CviJI RGCY 5 cut(s) 95, 193, 338, 506, 626
CviKI_1 RGCY 5 cut(s) 95, 193, 338, 506, 626
CviQI GTAC 2 cut(s) 394, 484
DdeI CTNAG 1 cut(s) 374
DpnI GATC 3 cut(s) 23, 99, 531
DpnII GATC 3 cut(s) 21, 97, 529
DraIII CACNNNGTG 1 cut(s) 179
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 234
Eco81I CCTNAGG 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 404
FaeI CATG 5 cut(s) 189, 251, 406, 461, 551
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FatI CATG 5 cut(s) 185, 247, 402, 457, 547
FbaI TGATCA 1 cut(s) 97
FblI GTMKAC 1 cut(s) 118
Fnu4HI GCNGC 8 cut(s) 93, 183, 345, 425, 504, 552, 555, 591
FokI GGATG 5 cut(s) 163, 411, 525, 589, 722
Fsp4HI GCNGC 8 cut(s) 93, 183, 345, 425, 504, 552, 555, 591
FspBI CTAG 3 cut(s) 507, 525, 558
GluI GCNGC 8 cut(s) 93, 183, 345, 425, 504, 552, 555, 591
HapII CCGG 1 cut(s) 623
Hin1II CATG 5 cut(s) 189, 251, 406, 461, 551
HincII GTYRAC 2 cut(s) 119, 140
HindII GTYRAC 2 cut(s) 119, 140
HinfI GANTC 2 cut(s) 115, 535
HpaII CCGG 1 cut(s) 623
Hpy166II GTNNAC 4 cut(s) 119, 140, 274, 394
Hpy188I TCNGA 2 cut(s) 418, 534
Hpy188III TCNNGA 4 cut(s) 6, 196, 466, 525
Hpy8I GTNNAC 4 cut(s) 119, 140, 274, 394
HpyCH4V TGCA 9 cut(s) 92, 185, 266, 290, 347, 402, 424, 593, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 1 cut(s) 374
Hsp92II CATG 5 cut(s) 189, 251, 406, 461, 551
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 3 cut(s) 21, 97, 529
Lsp1109I GCAGC 8 cut(s) 104, 169, 331, 436, 515, 538, 541, 577
LweI GCATC 3 cut(s) 275, 331, 389
MaeI CTAG 3 cut(s) 507, 525, 558
MalI GATC 3 cut(s) 23, 99, 531
MboI GATC 3 cut(s) 21, 97, 529
MluCI AATT 6 cut(s) 260, 411, 474, 497, 513, 606
MlyI GAGTC 1 cut(s) 124
MnlI CCTC 6 cut(s) 264, 310, 369, 474, 642, 703
Mph1103I ATGCAT 1 cut(s) 404
MseI TTAA 1 cut(s) 751
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 1 cut(s) 623
MspR9I CCNGG 1 cut(s) 147
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 344
NdeII GATC 3 cut(s) 21, 97, 529
NlaIII CATG 5 cut(s) 189, 251, 406, 461, 551
NlaIV GGNNCC 1 cut(s) 321
NsiI ATGCAT 1 cut(s) 404
NspI RCATGY 1 cut(s) 189
PctI GAATGC 1 cut(s) 224
PfeI GAWTC 1 cut(s) 535
PkrI GCNGC 8 cut(s) 94, 184, 346, 426, 505, 553, 556, 592
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 321
PstI CTGCAG 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 2 cut(s) 395, 485
RsaNI GTAC 2 cut(s) 394, 484
SalI GTCGAC 1 cut(s) 117
SaqAI TTAA 1 cut(s) 751
SatI GCNGC 8 cut(s) 93, 183, 345, 425, 504, 552, 555, 591
Sau3AI GATC 3 cut(s) 21, 97, 529
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 147
SetI ASST 9 cut(s) 97, 178, 195, 256, 321, 340, 375, 485, 508
SfaNI GCATC 3 cut(s) 275, 331, 389
SfcI CTRYAG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
Sse9I AATT 6 cut(s) 260, 411, 474, 497, 513, 606
SspMI CTAG 3 cut(s) 507, 525, 558
StyD4I CCNGG 1 cut(s) 145
TaqI TCGA 3 cut(s) 118, 611, 695
TasI AATT 6 cut(s) 260, 411, 474, 497, 513, 606
TfiI GAWTC 1 cut(s) 535
Tru1I TTAA 1 cut(s) 751
Tru9I TTAA 1 cut(s) 751
TseI GCWGC 8 cut(s) 92, 182, 344, 424, 503, 551, 554, 590
TspDTI ATGAA 5 cut(s) 68, 264, 378, 446, 527
TspGWI ACGGA 1 cut(s) 411
XapI RAATTY 2 cut(s) 260, 513
XbaI TCTAGA 1 cut(s) 524
XceI RCATGY 1 cut(s) 189
XcmI CCANNNNNNNNNTGG 2 cut(s) 281, 310
XmiI GTMKAC 1 cut(s) 118
XspI CTAG 3 cut(s) 507, 525, 558
Zsp2I ATGCAT 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.