MD03G1059400.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003044
Physical Location & Seq
Reverse (-)
4783785 .. 4784856
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1059400.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGGAATCATGAGCACTACTCGAATTTCGTCGTCATCGTCATCGTCGTTTGGTGTTCCTCATCAAGGGATGACGATGATGAGTACAACAACTTTGAACACAATCACACCTTGTGCTGCGTGTAAGCTCTTGAGAAGAAGATGTGCTGAAGAGTGCCCTTTCTCTCCCTACTTCTCTCCACATGAACCCCAGAAATTTTCAGCTGTTCACAAAGTCTTTGGTGCAAGCAATGTTTCCAAGATGCTACTGGAAGTGGCTGAGAATCAAAGAGCTGATGCAGCCAATAGTCTAGTTTATGAAGCAAATCTGAGGCTGAGAGACCCAGTGTATGGGTGCATGGGTGTAATTTCAGCCTTGCAGCAACAAATTCAATCTCTACAAGCTGAACTCAATGCAGTAAGGACTCAGATATTGAGATACAAATTCAAAGAGGCCACTAGTCCCAGTATCGTTTCTCCTAGGGGTATTATTCACCATGCTGCTTTGGTTTCTAATTCTGGGATGGTGTCCATTGCTGCGCAACCACAAGCGATTTCTCCACCACCGCCACCGCCACTACCACCAGCATCAGCTCCGTCTTCAGTTGTTTTTTCTTCATCGTCTTCCTCTTCAGCTTCTTCTTTATACAATCCGTCCACAAACACGACAGGCTATAGCTCCATCTCAAGCAAAAATGTTCCGTATTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

231

Amino Acids

24.52

Weight (kDa)

9.2

Isoelectric Point (pI)

70.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 37 - 134 2.2e-40 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 519
AccB7I CCANNNNNTGG 1 cut(s) 329
AciI CCGC 2 cut(s) 545, 551
AcsI RAATTY 4 cut(s) 24, 194, 366, 422
AcuI CTGAAG 3 cut(s) 168, 564, 594
AdeI CACNNNGTG 1 cut(s) 113
AfaI GTAC 1 cut(s) 85
AfiI CCNNNNNNNGG 2 cut(s) 65, 329
AgsI TTSAA 3 cut(s) 97, 371, 427
AhlI ACTAGT 1 cut(s) 437
AluBI AGCT 7 cut(s) 127, 203, 272, 383, 572, 614, 657
AluI AGCT 7 cut(s) 127, 203, 272, 383, 572, 614, 657
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 312
AoxI GGCC 1 cut(s) 432
ApeKI GCWGC 5 cut(s) 116, 278, 358, 479, 515
ApoI RAATTY 4 cut(s) 24, 194, 366, 422
AspA2I CCTAGG 1 cut(s) 458
AspLEI GCGC 1 cut(s) 520
AsuHPI GGTGA 1 cut(s) 464
AvrII CCTAGG 1 cut(s) 458
BaeGI GKGCMC 1 cut(s) 158
BbsI GAAGAC 2 cut(s) 570, 594
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 5 cut(s) 103, 290, 370, 466, 502
BccI CCATC 2 cut(s) 496, 668
BcoDI GTCTC 1 cut(s) 312
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 3 cut(s) 290, 438, 459
BfmI CTRYAG 1 cut(s) 652
BisI GCNGC 5 cut(s) 117, 279, 359, 480, 516
BlnI CCTAGG 1 cut(s) 458
BlsI GCNGC 5 cut(s) 118, 280, 360, 481, 517
BmrI ACTGGG 2 cut(s) 317, 438
BmsI GCATC 3 cut(s) 231, 265, 575
BmuI ACTGGG 2 cut(s) 317, 438
BpiI GAAGAC 2 cut(s) 570, 594
BplI GAGNNNNNCTC 1 cut(s) 36
BpuEI CTTGAG 2 cut(s) 151, 649
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 458
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 329
Bse1I ACTGG 3 cut(s) 252, 323, 444
Bse3DI GCAATG 2 cut(s) 235, 510
BseDI CCNNGG 1 cut(s) 458
BseGI GGATG 2 cut(s) 75, 507
BseLI CCNNNNNNNGG 2 cut(s) 65, 329
BseMI GCAATG 2 cut(s) 235, 510
BseMII CTCAG 4 cut(s) 249, 299, 305, 419
BseNI ACTGG 3 cut(s) 252, 323, 444
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 5 cut(s) 103, 290, 370, 466, 502
BshFI GGCC 1 cut(s) 434
BsiHKAI GWGCWC 1 cut(s) 17
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 2 cut(s) 65, 329
BsmAI GTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 426
BsnI GGCC 1 cut(s) 434
Bso31I GGTCTC 1 cut(s) 312
Bsp1286I GDGCHC 2 cut(s) 17, 158
BspACI CCGC 2 cut(s) 545, 551
BspANI GGCC 1 cut(s) 434
BspCNI CTCAG 4 cut(s) 250, 300, 306, 418
BspHI TCATGA 1 cut(s) 9
BspTNI GGTCTC 1 cut(s) 312
BsrDI GCAATG 2 cut(s) 235, 510
BsrI ACTGG 3 cut(s) 252, 323, 444
BssECI CCNNGG 1 cut(s) 458
BssT1I CCWWGG 1 cut(s) 458
Bst6I CTCTTC 2 cut(s) 144, 613
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 4 cut(s) 258, 308, 314, 405
BstENI CCTNNNNNAGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 75, 507
BstHHI GCGC 1 cut(s) 520
BstMAI GTCTC 1 cut(s) 312
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstSFI CTRYAG 1 cut(s) 652
BstSLI GKGCMC 1 cut(s) 158
BstV1I GCAGC 5 cut(s) 103, 290, 370, 466, 502
BstV2I GAAGAC 2 cut(s) 570, 594
BsuRI GGCC 1 cut(s) 434
BtsCI GGATG 2 cut(s) 75, 507
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 226
CciI TCATGA 1 cut(s) 9
CfoI GCGC 1 cut(s) 520
Csp6I GTAC 1 cut(s) 84
CviAII CATG 4 cut(s) 10, 182, 337, 476
CviQI GTAC 1 cut(s) 84
DdeI CTNAG 4 cut(s) 258, 308, 314, 405
DraIII CACNNNGTG 1 cut(s) 113
Eam1104I CTCTTC 2 cut(s) 144, 613
EarI CTCTTC 2 cut(s) 144, 613
Eco130I CCWWGG 1 cut(s) 458
Eco31I GGTCTC 1 cut(s) 312
Eco57I CTGAAG 3 cut(s) 168, 564, 594
EcoNI CCTNNNNNAGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 458
ErhI CCWWGG 1 cut(s) 458
FaeI CATG 4 cut(s) 13, 185, 340, 479
FaiI YATR 8 cut(s) 11, 183, 297, 330, 338, 477, 625, 654
FaqI GGGAC 1 cut(s) 426
FatI CATG 4 cut(s) 9, 181, 336, 475
Fnu4HI GCNGC 5 cut(s) 117, 279, 359, 480, 516
FokI GGATG 2 cut(s) 82, 514
Fsp4HI GCNGC 5 cut(s) 117, 279, 359, 480, 516
FspBI CTAG 3 cut(s) 290, 438, 459
FspI TGCGCA 1 cut(s) 519
GlaI GCGC 1 cut(s) 519
GluI GCNGC 5 cut(s) 117, 279, 359, 480, 516
HaeIII GGCC 1 cut(s) 434
HhaI GCGC 1 cut(s) 520
Hin1II CATG 4 cut(s) 13, 185, 340, 479
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HinfI GANTC 3 cut(s) 6, 262, 403
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 2 cut(s) 208, 636
Hpy188I TCNGA 2 cut(s) 309, 408
Hpy188III TCNNGA 2 cut(s) 10, 130
Hpy8I GTNNAC 2 cut(s) 208, 636
Hpy99I CGWCG 2 cut(s) 34, 49
HpyCH4V TGCA 5 cut(s) 224, 278, 336, 358, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 4 cut(s) 258, 308, 314, 405
Hsp92II CATG 4 cut(s) 13, 185, 340, 479
HspAI GCGC 1 cut(s) 518
LmnI GCTCC 2 cut(s) 577, 662
LpnPI CCDG 7 cut(s) 203, 233, 336, 457, 483, 576, 633
Lsp1109I GCAGC 5 cut(s) 103, 290, 370, 466, 502
LweI GCATC 3 cut(s) 231, 265, 575
MaeI CTAG 3 cut(s) 290, 438, 459
MboII GAAGA 8 cut(s) 147, 150, 161, 570, 585, 594, 600, 609
MhlI GDGCHC 2 cut(s) 17, 158
MluCI AATT 6 cut(s) 24, 194, 345, 366, 422, 493
MlyI GAGTC 1 cut(s) 397
MnlI CCTC 4 cut(s) 69, 303, 424, 616
MseI TTAA 1 cut(s) 691
MspA1I CMGCKG 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 278
NlaIII CATG 4 cut(s) 13, 185, 340, 479
NsbI TGCGCA 1 cut(s) 519
PagI TCATGA 1 cut(s) 9
PcsI WCGNNNNNNNCGW 2 cut(s) 35, 44
PfeI GAWTC 2 cut(s) 6, 262
PflMI CCANNNNNTGG 1 cut(s) 329
PkrI GCNGC 5 cut(s) 118, 280, 360, 481, 517
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 1 cut(s) 85
RsaNI GTAC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 691
SatI GCNGC 5 cut(s) 117, 279, 359, 480, 516
SchI GAGTC 1 cut(s) 397
SduI GDGCHC 2 cut(s) 17, 158
SetI ASST 8 cut(s) 112, 129, 205, 274, 385, 574, 616, 659
SfaNI GCATC 3 cut(s) 231, 265, 575
SfcI CTRYAG 1 cut(s) 652
SmlI CTYRAG 2 cut(s) 130, 664
SmoI CTYRAG 2 cut(s) 130, 664
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 6 cut(s) 24, 194, 345, 366, 422, 493
SsiI CCGC 2 cut(s) 545, 551
SspMI CTAG 3 cut(s) 290, 438, 459
StyI CCWWGG 1 cut(s) 458
TaqI TCGA 1 cut(s) 22
TasI AATT 6 cut(s) 24, 194, 345, 366, 422, 493
TatI WGTACW 1 cut(s) 83
TfiI GAWTC 2 cut(s) 6, 262
Tru1I TTAA 1 cut(s) 691
Tru9I TTAA 1 cut(s) 691
TscAI CASTG 1 cut(s) 330
TseI GCWGC 5 cut(s) 116, 278, 358, 479, 515
TspDTI ATGAA 3 cut(s) 198, 312, 585
TspGWI ACGGA 3 cut(s) 564, 621, 669
TspRI CASTG 1 cut(s) 330
Van91I CCANNNNNTGG 1 cut(s) 329
XagI CCTNNNNNAGG 1 cut(s) 63
XapI RAATTY 4 cut(s) 24, 194, 366, 422
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmaJI CCTAGG 1 cut(s) 458
XspI CTAG 3 cut(s) 290, 438, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.